FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_16_SL248345.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_16_SL248345.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6871328
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32476 0.4726306181279659 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25758 0.37486203540276347 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24416 0.3553316040218136 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22371 0.3255702536685776 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21703 0.31584869766077245 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21620 0.3146407797735751 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21438 0.31199209235827485 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20765 0.3021977702126867 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20678 0.30093163941526296 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19980 0.2907734865807599 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19552 0.28454470518653746 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19031 0.2769624736295517 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18152 0.264170186607305 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17594 0.25604948563072527 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17467 0.2542012257310377 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17236 0.2508394301654644 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17191 0.25018453492541765 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17022 0.24772503946835314 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17020 0.24769593301323997 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16444 0.23931327394064147 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15861 0.2308287422751468 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15745 0.22914056787858186 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15446 0.22478915283916007 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15136 0.22027765229661575 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14915 0.21706138900660835 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14774 0.21500938392112848 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14513 0.21121099152885728 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14460 0.21043967046835782 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14375 0.20920264612604725 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13924 0.2026391404980231 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13848 0.20153309520372192 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13760 0.20025241117874157 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 13670 0.19894262069864807 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13509 0.1965995510620363 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13230 0.19253920057374646 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13182 0.1918406456510299 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12843 0.18690710150934434 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12698 0.18479688351363813 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 12368 0.1799943184199619 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12322 0.17932486995235858 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12239 0.1781169520651612 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12126 0.176472437351266 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12109 0.17622503248280388 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11364 0.16538287795314094 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11354 0.165237345677575 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 11191 0.16286516958585007 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11040 0.1606676322248043 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10706 0.1558068542209017 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10646 0.15493366056750602 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10332 0.1503639471147353 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10247 0.14912692277242479 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10131 0.1474387483758598 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10078 0.14666742731536028 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9819 0.14289814137820228 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9810 0.14276716233019293 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9484 0.1380228101467431 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9344 0.13598535828881986 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9046 0.13164849647695467 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8736 0.12713699593441036 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8701 0.12662763296992954 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 8467 0.12322217772168642 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7849 0.11422828309171096 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7801 0.11352972816899441 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7733 0.11254010869514597 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7666 0.11156504244885415 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 7614 0.11080827461591122 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 7354 0.10702443545119661 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 7324 0.10658783862449879 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 13946885 7.6700416 65.43638 35-39
TATTA 6934025 7.3515306 127.709 240-247
TGGAT 10541250 7.1876535 77.92839 35-39
TCACC 10821870 6.460292 74.78701 160-169
TCTCC 9518200 6.097974 85.12311 20-24
CTGTG 10083095 5.9510727 86.49436 25-29
TCCCT 9194825 5.890799 74.5293 15-19
AGTGG 10954135 5.7820787 40.545506 100-109
CTGAG 10178465 5.597612 50.468925 15-19
TGTAT 6344080 5.587954 60.24992 240-247
TTACT 5846105 5.3649464 112.46431 240-247
ATTAC 5877790 5.026109 106.560905 240-247
TGGGT 8665480 4.9088464 45.69566 100-109
CCTGA 8465440 4.8504806 51.988747 15-19
CTCTG 7678520 4.721648 74.17953 35-39
ACACG 8735215 4.6636705 49.382442 230-239
TTCAC 6295610 4.6597447 74.0698 40-44
GACAC 8655830 4.6212873 46.31446 230-239
CTCCA 7685650 4.588074 41.31225 80-89
TGAGA 6928880 4.4022746 93.40347 15-19
ATTCA 5074090 4.3388643 112.32126 40-44
TACTG 6093915 4.329184 79.48138 240-247
TCTGG 7256915 4.283054 135.36168 35-39
AAGGG 8701960 4.279983 39.813725 90-99
CCCTG 8505285 4.218232 84.99679 10-14
TGGAG 7925160 4.1832514 38.845543 90-99
CAGCC 8936685 4.129877 55.500004 30-34
GAAGG 8378620 4.1209517 31.294365 90-99
AGCCT 7178305 4.112985 50.385544 30-34
CCAGG 9228545 4.093354 53.722256 80-89
TCAGT 5738150 4.0764446 93.0706 7
ACCAT 5910200 4.0761065 67.54275 160-169
GAGAC 7862625 4.029093 88.71659 8
ACTAC 5787320 3.9913602 36.06062 130-139
GTATT 4519975 3.9812567 70.29518 240-247
CACCA 7132130 3.967237 57.293076 160-169
TCCAG 6870935 3.9368694 40.633003 80-89
TACTA 4591645 3.9263246 28.644869 130-139
GGGTC 8531280 3.897863 121.702194 8
CACCT 6357885 3.795443 44.98685 20-24
ACTGT 5306380 3.769711 68.52903 240-247
CTGGG 8203530 3.7481163 129.22235 3
GACTC 6502495 3.7257633 52.288727 20-24
GATTC 5234680 3.7187746 60.04786 40-44
TGAAG 5844670 3.7134204 39.461227 150-159
AGACT 5558955 3.679781 67.53552 15-19
CAAGA 5957710 3.6747448 44.610134 180-189
GGACA 7029310 3.6020718 27.923222 220-229
GCTGG 7819490 3.5726519 33.365696 90-99
ACTGG 6445395 3.5446236 44.498512 60-69
CCTGT 5739065 3.5290456 62.219593 25-29
GAGTG 6510400 3.4364781 39.33128 100-109
ACTCT 4584800 3.3934753 68.81288 20-24
TATCT 3657525 3.3564956 49.181305 200-209
GGATT 4844040 3.3029556 57.301647 40-44
TCCTG 5366155 3.299737 62.607525 20-24
AGACA 5337995 3.292502 38.39756 170-179
AGCTG 5975060 3.2859643 37.294353 60-69
GGTCC 6778265 3.226603 138.82135 9
CTGCA 5612600 3.2158756 46.34411 25-29
CTCCT 5013820 3.212177 92.06517 20-24
CATCT 4261410 3.1541157 33.17488 170-179
CCATC 5245720 3.1315184 36.1129 45-49
GTCTC 5050640 3.1057217 33.65901 30-34
GTCCC 6197670 3.0737607 74.388626 10-14
CCTCA 5109865 3.0504174 78.80051 5
GGAGT 5691290 3.0041153 37.21567 90-99
AAGAA 4179570 2.9783313 37.95668 180-189
GGCTG 6426875 2.9363797 34.343952 90-99
AGGGA 5934570 2.9188666 40.334934 80-89
AGTAG 4583870 2.912369 29.292152 50-59
GAGCT 5248525 2.8864088 30.350039 60-69
GTGTA 4162305 2.838108 36.603786 230-239
TGTGC 4792395 2.8284864 56.70466 25-29
ATCTC 3781700 2.7990546 48.07757 170-179
GGAAG 5677290 2.7923255 30.113155 80-89
ATCTG 3894935 2.7670045 37.07626 200-209
GTGAA 4331255 2.7518697 38.695557 150-159
CAGGG 6413305 2.7303185 36.407406 80-89
CTATG 3812235 2.7082536 28.250544 50-59
GACAA 4381360 2.7024446 24.894278 180-189
ACCTT 3638745 2.6932454 70.50978 40-44
GAACA 4353700 2.6853838 34.903584 210-219
GGGAA 5375535 2.6439102 33.667645 80-89
CCCTC 5023645 2.5958238 28.162592 20-24
ATGAA 3381980 2.586386 27.96782 210-219
GAGTC 4689320 2.578876 28.689795 150-159
AGAAC 4178700 2.577443 30.957985 190-199
CACGG 5793500 2.5697274 44.437317 230-239
TATAT 2395505 2.539741 24.605305 240-247
ATCAG 3816435 2.52631 27.656048 45-49
TGCAA 3792295 2.5103304 35.711346 200-209
CGCCA 5413315 2.5016356 39.650875 70-79
CAGTG 4543320 2.4985833 72.09488 8
CCAAG 4672985 2.4948745 39.1065 180-189
AGGGC 5835745 2.4844358 32.124413 150-159
ACGGC 5537505 2.45618 49.305088 230-239
CTACA 3561075 2.4559782 19.204924 190-199
AGGCT 4436590 2.439888 20.360922 80-89
GTGGA 4595595 2.4257588 27.136152 100-109
CTGTC 3940240 2.422918 50.36179 30-34
TCTGT 3153405 2.4041984 25.586998 210-219
CACTG 4171475 2.3901482 20.364801 30-34
GCAGC 5371005 2.3823283 34.806423 30-34
CTACT 3201605 2.3696928 23.425545 50-59
CCGCC 5904290 2.361755 33.945217 70-79
TCCAA 3399695 2.3446786 44.295692 180-189
TATGC 3298940 2.3436034 21.568647 50-59
CTCTC 3646555 2.3362188 97.07679 20-24
GTATC 3281985 2.3315585 23.29199 200-209
TGAAC 3514935 2.3267305 37.82313 210-219
GTGGT 4105415 2.3256476 31.400404 120-129
ACAGC 4310055 2.3011084 27.015749 210-219
GGGCT 5013405 2.2905784 42.44584 90-99
CTTCA 3093550 2.289715 50.905693 45-49
CAAAT 2865235 2.2829542 34.74427 200-209
TGGTG 4026260 2.2808075 35.007057 35-39
GGCCG 6125345 2.2571914 25.356865 230-239
CAGGC 5066920 2.2474504 32.66928 70-79
CAGTA 3380495 2.237737 22.132818 45-49
GCTCC 4470900 2.2173617 34.09931 80-89
TATCA 2593005 2.2172837 17.64911 170-179
AATGA 2897925 2.2162027 26.334454 210-219
GCCTC 4459140 2.2115293 69.32195 4
ACAAC 3425710 2.2014713 19.455713 180-189
AAATG 2873360 2.1974165 32.957264 200-209
GCCTG 4592570 2.186164 30.379951 210-219
CCTTC 3352605 2.1478956 56.3419 45-49
GTGGG 4881950 2.1408734 30.102242 100-109
GGGGG 6263930 2.1264515 91.932465 5
TACAT 2480010 2.1206615 17.904568 190-199
GCTGT 3591455 2.1196876 19.485 230-239
GCTAT 2983555 2.1195502 34.49044 50-59
GTAGT 3087650 2.105344 25.138361 120-129
TGCAG 3797795 2.0885847 43.92523 30-34
TCTAT 2272170 2.085161 20.810163 110-119
GCAGA 4061155 2.081082 24.459057 140-149
CCTCT 3220440 2.0632222 49.139606 35-39
TGCAC 3578995 2.0506725 27.290035 25-29
CTCAC 3419955 2.0415978 56.652615 20-24
AACTA 2544680 2.0275433 37.44735 130-139
AACAG 3281690 2.0241625 33.62443 210-219
AGCAC 3748450 2.0012712 21.194294 130-139
AGAGT 3145570 1.998543 30.77212 150-159
TGTGA 2922235 1.9925543 19.844063 210-219
GGCTC 4176640 1.9881723 20.557758 40-44
ATATC 2316960 1.9812368 14.254491 160-169
TGGTA 2904965 1.9807786 31.11723 120-129
TGAGC 3575360 1.9662573 18.126377 200-209
CAGAG 3824055 1.9595835 32.372574 170-179
CATAT 2290480 1.958594 15.016448 160-169
ATGGA 3072590 1.9521747 12.135422 120-129
TTCAG 2739145 1.9459187 47.669136 45-49
CCGTG 4081510 1.9428884 20.268145 230-239
TCTCT 2441965 1.9397451 33.571953 35-39
TCCGC 3907835 1.9381073 41.886505 70-79
CAGAC 3626745 1.9362938 26.63312 140-149
GGTGG 4411590 1.9346071 19.019798 40-44
CTGTA 2715135 1.9288616 24.38968 190-199
GGTCT 3236920 1.9104402 34.91596 15-19
GGATC 3449595 1.8970933 18.533094 70-79
CAGTT 2659445 1.8892989 25.510231 190-199
ACTAT 2192290 1.8746315 17.141874 130-139
ACCAG 3510475 1.8742181 14.3506565 190-199
GCACA 3506880 1.8722988 18.527378 130-139
GCCAG 4208530 1.8667084 35.47128 70-79
CCAGA 3492880 1.8648247 33.938274 170-179
ACCCT 3123545 1.8646511 44.558605 10-14
TGGGG 4241825 1.8601605 119.55258 4
CCTGC 3740850 1.8552904 49.139244 20-24
GTCAC 3236925 1.8546753 38.16941 160-169
GCTGA 3370350 1.8535124 27.37134 200-209
AAAAA 2160620 1.8532244 11.648268 90-99
TCCAT 2500690 1.8509052 27.843924 45-49
CTATT 2009570 1.8441739 20.302017 110-119
TGTCT 2412515 1.839334 51.00804 30-34
AGAGA 3102995 1.8370218 27.677181 170-179
AGGAC 3567175 1.8279492 20.040762 220-229
GATGG 3446215 1.8190653 20.497711 100-109
TGGGA 3444880 1.8183604 16.311117 100-109
ATACT 2103980 1.7991172 26.76517 130-139
TAGTG 2627060 1.7912862 26.543478 120-129
TTCCA 2413995 1.7867372 23.579803 180-189
TCAGC 3115950 1.7853594 11.118838 45-49
GCCGA 4014015 1.7804304 19.31224 220-229
GAGGA 3614750 1.7778834 26.360884 220-229
TACAA 2214150 1.7641846 18.542189 130-139
CACAG 3299685 1.7616788 15.479197 140-149
ACCTG 3073295 1.7609191 31.959864 25-29
CAAGG 3429315 1.7573045 17.064762 80-89
AGTCA 2643310 1.7497535 29.535162 160-169
AGTTC 2439330 1.732927 22.820034 190-199
AGCTA 2605835 1.7249467 26.587666 50-59
TGACC 3005165 1.7218827 14.952839 210-219
GCAAA 2788000 1.7196522 32.277184 200-209
CTGAA 2594325 1.7173275 23.876682 200-209
CCTGG 3595735 1.7116488 34.34686 2
TAGTA 2079730 1.7069083 16.731682 120-129
ATGCA 2566360 1.6988158 17.005346 60-69
TTCTG 2222800 1.6946927 41.84077 30-34
GGAGC 3975100 1.6923083 12.783736 200-209
CTCAG 2936440 1.6825048 75.32132 6
CATGA 2537365 1.6796227 22.96228 60-69
GTGCA 3051450 1.6781348 49.42463 25-29
ATTGG 2433315 1.6591794 21.508024 100-109
GCCGT 3449610 1.64209 29.472088 230-239
AACAC 2545110 1.6355696 22.878387 190-199
TCTCA 2184330 1.6167488 26.497421 100-109
CTCCC 3126510 1.615534 14.643135 190-199
GGATG 3052650 1.6113242 21.698704 100-109
TCTGC 2615400 1.6082525 33.520348 200-209
AGGGG 3920765 1.6020926 32.888042 90-99
CAGCT 2792950 1.6002886 13.231941 50-59
GTTCT 2081565 1.5870132 24.208725 190-199
GCACT 2768460 1.5862565 26.279331 25-29
CGATT 2217335 1.575219 34.97984 160-169
GGGGC 4440425 1.5705354 28.654713 90-99
TTCTC 1975530 1.5692381 23.176626 190-199
GGGCC 4253290 1.5673387 26.568268 2
ACAAT 1960345 1.5619584 23.677954 180-189
CGTCC 3145535 1.5600413 19.681261 180-189
TCTGA 2176000 1.5458542 20.984467 220-229
AGTTA 1881545 1.544251 16.137058 50-59
CGGCC 3938290 1.5120292 31.77073 230-239
CATCA 2179040 1.5028256 32.07905 45-49
CCATG 2622535 1.5026455 15.433725 160-169
GAGCA 2914075 1.4932771 7.510225 200-209
GTTAC 2095495 1.4886628 16.885569 50-59
GGCTT 2514740 1.4842073 20.727606 25-29
GTCCG 3110035 1.4804449 34.10493 70-79
CTGGT 2498755 1.4747727 32.057564 35-39
AGTGA 2308230 1.4665376 82.97854 9
TTAGT 1663115 1.4648948 25.420883 110-119
ACTCC 2439805 1.4564813 17.16532 140-149
CTTTA 1580510 1.4504275 41.121906 45-49
CTCAA 2077870 1.4330513 19.111605 150-159
GTCCA 2488955 1.4261075 29.658854 180-189
TACGC 2488375 1.4257753 24.448238 140-149
GTGAC 2590545 1.4246616 15.852788 210-219
AAGAG 2399170 1.4203464 19.594814 150-159
GGCAG 3335305 1.4199301 13.30555 150-159
TAGCA 2138480 1.4155784 12.244636 130-139
CAACC 2533250 1.4091166 15.697781 140-149
CGTGT 2379590 1.4044411 24.215895 230-239
AAGCT 2106640 1.3945019 17.461388 200-209
AACCC 2499340 1.3902543 20.838562 140-149
GGGGT 3141365 1.3775777 114.38112 7
CAACT 1975585 1.362508 18.396994 130-139
CTACG 2368210 1.3569236 16.049986 130-139
GAGAG 2753355 1.3542138 25.783304 220-229
CAATT 1579400 1.350548 25.118399 180-189
GGCCT 2834090 1.3490889 66.35959 3
CACGT 2345765 1.3440632 16.968372 180-189
TGGAA 2112815 1.3423803 20.082348 120-129
AGCAG 2617430 1.3412654 12.70792 50-59
ATCTA 1560810 1.3346517 21.272762 110-119
TCAAG 2006135 1.3279719 23.984592 150-159
GAACT 1999650 1.3236794 14.321825 190-199
TGTCC 2150565 1.3224179 55.74178 15-19
GTGGC 2894005 1.3222439 14.871894 40-44
ACAGT 1994105 1.3200088 12.422673 120-129
AACCA 2049830 1.3172867 17.811607 190-199
TTACA 1533675 1.3114485 10.024531 120-129
CGACA 2455290 1.3108622 15.764824 70-79
TGTGT 1791105 1.310681 15.380018 240-247
AGAGC 2551495 1.3074781 24.915014 220-229
AATTC 1527970 1.3065702 25.110584 180-189
CCATA 1891405 1.3044516 15.521351 160-169
GTGCG 2839415 1.2973025 17.28714 240-247
CCTTT 1621420 1.2879552 48.291523 45-49
CCAGT 2245180 1.2864307 19.53081 190-199
AGACC 2401255 1.2820135 100.8846 9
TCCGT 2084240 1.2816334 14.188227 150-159
ATCAT 1494565 1.2780055 16.446985 110-119
ATACA 1599690 1.2745967 21.360683 35-39
TTATT 1118190 1.2722981 19.39721 240-247
ACAGA 2058765 1.2698565 19.661755 140-149
ACATG 1913020 1.266334 14.072623 190-199
GATTG 1852510 1.2631519 24.509987 100-109
TCACT 1702745 1.2602999 12.8781 190-199
ACGTC 2198445 1.2596525 18.830187 180-189
ATATT 1187720 1.259234 26.253235 240-247
ATCCG 2187145 1.2531779 24.344643 70-79
TGCGA 2277655 1.2525887 14.218372 240-247
ACATA 1564660 1.2466856 20.861303 130-139
CGGCT 2590910 1.23333 21.337786 230-239
CCTAC 2065805 1.233216 16.791204 190-199
TCGGA 2236180 1.2297795 95.392296 5
ATTAT 1158770 1.2285409 9.694505 50-59
AGTCG 2233240 1.2281629 16.825764 150-159
CTTCT 1506705 1.1968327 22.919682 30-34
CGCTG 2510820 1.1952053 12.970859 190-199
GTCGA 2164900 1.1905795 13.06769 150-159
CTTCG 1935245 1.190014 107.556564 3
GATCC 2073815 1.1882429 24.208271 70-79
GATCT 1663080 1.1814704 9.289174 220-229
ACACA 1826055 1.1734817 7.565707 130-139
TGAGT 1715065 1.1694337 20.410675 90-99
ATTAG 1424770 1.1693593 25.065046 110-119
TGGAC 2126235 1.1693158 15.600466 80-89
ATTCC 1577015 1.1672399 23.200788 180-189
TACCA 1688835 1.1647443 10.6933565 45-49
AGCTC 2019355 1.1570388 12.770029 210-219
ACGCA 2155845 1.1509906 20.948895 140-149
GGGTG 2623765 1.1505954 14.219732 60-69
GACCC 2483380 1.1476353 44.045322 10-14
GAAGT 1805355 1.1470352 13.049575 140-149
TCGAG 2083040 1.1455607 12.7239895 150-159
AGATC 1728100 1.1439253 12.34245 210-219
AAGGC 2229945 1.1427041 28.023209 25-29
GAACC 2128345 1.1363088 11.038271 180-189
TTCGG 1924610 1.1359107 103.43461 4
TTGGG 2004920 1.1357529 15.982256 100-109
GGACT 2060830 1.1333464 13.409263 90-99
GTATA 1374005 1.1276948 10.630726 240-247
GCCCC 2809070 1.1236465 12.595044 80-89
AGGCC 2520830 1.1181232 13.746663 2
GCAAG 2177600 1.1158807 16.66928 25-29
CCCCT 2134555 1.1029698 10.180271 140-149
GAAGC 2146545 1.0999671 13.616059 200-209
ACAAG 1781925 1.0991001 17.12149 80-89
CGCAG 2471615 1.0962936 16.433657 140-149
AGGTC 1990700 1.0947789 17.681637 10-14
TAGAC 1648875 1.0914817 18.246292 170-179
GCACC 2360855 1.0910132 11.455148 130-139
CCGAT 1895935 1.0863223 23.505348 160-169
ATCAA 1351035 1.0764741 13.00292 110-119
CACAT 1557765 1.0743488 13.9025 130-139
GGAGA 2168075 1.0663489 84.922165 7
TTGAG 1561285 1.0645773 20.182877 90-99
GCTTC 1730850 1.0643281 27.450026 2
TGGCT 1798070 1.0612265 32.259052 40-44
CTTGA 1493115 1.0607252 21.780096 90-99
TACAG 1598840 1.0583609 9.381527 120-129
CTCCG 2133905 1.0583192 10.030405 150-159
GTAGA 1664900 1.0577968 17.726686 170-179
GTAGC 1911490 1.0512176 11.75224 50-59
CTATA 1226455 1.048744 13.469515 50-59
GCTCT 1703140 1.0472887 17.97402 210-219
ATGGT 1530200 1.0433816 9.871336 35-39
ATGGG 1957975 1.033506 19.317966 100-109
CGTGA 1878770 1.0332232 20.974596 150-159
CAGCA 1933355 1.0322047 11.965554 45-49
TAGTT 1171400 1.0317852 15.296775 50-59
GAGCC 2319875 1.0289888 17.89467 220-229
ACCGC 2223205 1.0274014 11.370901 220-229
ATGAG 1612665 1.0246092 19.819601 60-69
TTATA 964460 1.0225313 14.552877 110-119
GGGAG 2493800 1.0190098 14.788732 5
ACCAA 1563335 1.0046494 17.741325 130-139
ATGAT 1221270 1.0023396 9.838051 120-129
GACAG 1943135 0.99573237 15.189975 70-79
AGCCG 2240715 0.9938772 18.91173 220-229
ACACC 1785880 0.9933932 12.620418 40-44
CGAGT 1804635 0.99245286 15.345048 160-169
AAACT 1241380 0.9891035 16.85643 130-139
TGAGG 1873155 0.9887344 11.71771 220-229
CGAGG 2313650 0.9849838 14.243885 220-229
TTTCA 1071910 0.9836874 6.937681 100-109
GGTAT 1429120 0.97445935 9.643657 110-119
CCAAC 1747500 0.9720445 14.72195 130-139
ACTCA 1404440 0.9686047 11.90281 190-199
GGGAC 2262380 0.96315676 10.313612 90-99
ATGAC 1453825 0.9623673 10.739254 160-169
TTTAG 1090870 0.96085346 29.368425 45-49
AACTC 1386970 0.9565562 15.967376 190-199
CCGTC 1924350 0.9543896 10.293831 140-149
AGCCC 2062450 0.95311254 11.7554865 80-89
GCAGT 1727240 0.9498897 6.0769925 50-59
CCGAG 2137965 0.94830203 17.821566 220-229
TATGG 1387405 0.9460156 12.826084 35-39
AAGGT 1487270 0.9449393 20.224878 15-19
CGGAC 2124070 0.9421388 11.116847 220-229
AATCA 1176345 0.937285 14.502614 110-119
GCCAA 1751715 0.9352284 14.357806 180-189
GCCAT 1631085 0.93456984 7.882286 60-69
TCATA 1092650 0.93432707 9.203832 110-119
CGTAT 1310540 0.931022 7.8441887 240-247
CATAC 1339245 0.9236415 14.243253 130-139
GATAC 1393695 0.922564 22.957605 35-39
CCCCA 1915230 0.9221388 17.279747 80-89
CCCAG 1994810 0.92185414 15.067998 80-89
CAGAA 1494420 0.9217656 17.6433 140-149
TGGTT 1255650 0.9188499 9.776142 50-59
TTTAT 802965 0.91362906 9.970171 240-247
ACAGG 1773045 0.90857214 14.755981 70-79
ACGCT 1566665 0.8976588 15.850223 190-199
GTTAT 1016670 0.8954971 11.711338 110-119
CGGAG 2100840 0.8943848 73.785675 6
AACTG 1350550 0.894004 11.001233 60-69
GACTG 1619685 0.8907404 12.990187 90-99
GAGAT 1398305 0.8884154 11.831347 210-219
TACAC 1288100 0.8883682 21.451881 35-39
TCCTT 1108195 0.88028115 11.037243 45-49
TAACA 1102665 0.8785785 8.220477 120-129
GGTAG 1659350 0.87587863 12.359428 120-129
TATTT 766815 0.87249684 10.574099 240-247
AAGTT 1057895 0.868252 16.853956 140-149
ATAGT 1051645 0.8631224 16.009893 120-129
ATATG 1036335 0.85055697 5.916435 50-59
AGAAG 1436100 0.85019374 15.342376 140-149
TGGCA 1540195 0.8470251 6.2604833 100-109
GATGA 1325015 0.84185034 6.369409 60-69
TCTTC 1056655 0.839341 49.15301 2
GATCA 1267930 0.8393132 9.328877 110-119
GTCTG 1418330 0.8371028 15.223376 3
AGTCT 1166720 0.82885075 15.569202 8
GCCTA 1446095 0.8285753 16.243635 190-199
ATCCA 1197940 0.82618713 9.577132 180-189
TTACC 1115350 0.825535 9.755769 45-49
GTCTA 1160155 0.8241869 10.362524 30-34
CATGG 1498315 0.8239933 8.519754 200-209
CATTA 963225 0.8236556 11.80262 110-119
CTATC 1110295 0.8217934 8.025226 110-119
GGTTC 1385715 0.8178533 11.256993 160-169
GCTTG 1374045 0.8109655 16.661032 90-99
TGATG 1186020 0.80869925 12.446656 120-129
ATATA 816605 0.8067212 10.360185 130-139
ACAAA 1083910 0.80472827 12.7396 130-139
ACATC 1155410 0.79685545 5.8038177 110-119
TCATC 1070625 0.79243135 10.314805 110-119
GGTGC 1732220 0.7914352 11.350379 60-69
CCTCC 1524285 0.7876304 11.186183 140-149
TATAC 910915 0.7789251 8.734504 130-139
TTATG 882385 0.777217 10.947781 50-59
GGTTA 1117960 0.7622919 8.67532 50-59
CTGAC 1324075 0.758661 9.657801 220-229
CGCCG 1967215 0.75527364 11.357455 220-229
CCCCC 1801600 0.7508275 13.75373 80-89
CCAGC 1623925 0.75045854 6.1283593 80-89
ATAAA 807020 0.74287343 8.6490135 130-139
GCGGA 1735260 0.73874736 9.374266 220-229
GTTCA 1038315 0.73763037 13.660174 160-169
GACCG 1660025 0.73631 9.778205 210-219
TTGGA 1079780 0.73625845 6.473471 60-69
TATGA 895360 0.7348538 5.2966957 120-129
TGACT 1030250 0.731901 5.13704 2
TCCGG 1526275 0.7265403 12.2969885 70-79
TCAAC 1051790 0.7253914 7.8313684 110-119
ATCTT 790400 0.7253468 13.818022 3
GGGAT 1368710 0.7224659 12.890074 100-109
GATTA 878720 0.7211968 5.8827763 50-59
ATGCC 1248225 0.7152009 8.15612 50-59
AAATA 776705 0.71496814 13.190921 130-139
CATCC 1195935 0.71393293 6.499715 110-119
AATAC 887140 0.7068531 11.818628 130-139
TGCCA 1231555 0.7056495 7.5537343 60-69
AACGC 1319925 0.70469874 14.128979 180-189
CACAA 1091345 0.7013334 8.440147 130-139
ACGCC 1514735 0.69999886 12.633236 180-189
TTTAC 760475 0.6978848 13.234663 45-49
GACCA 1297955 0.69296926 6.075722 170-179
GAAAT 904305 0.69157183 7.5310388 110-119
AATAA 750330 0.69068956 8.183752 130-139
GGGCA 1620135 0.6897356 13.262651 150-159
TATTG 779580 0.6866649 6.273032 240-247
GGATA 1077630 0.6846739 14.880425 35-39
TTTCT 690605 0.6801574 9.9409 4
CAACG 1272895 0.67958975 14.386879 180-189
GCCGC 1766050 0.67804027 12.42005 220-229
AGGCA 1322790 0.6778453 7.020961 80-89
CACGC 1465770 0.67737085 12.885506 190-199
CAAAC 1052780 0.67655027 13.644255 130-139
TATAG 813970 0.6680541 6.0176725 120-129
CCCGT 1322810 0.65605325 10.117943 140-149
CAGTC 1139395 0.6528441 5.2800274 210-219
CCGGC 1672370 0.64207375 9.983494 70-79
ATTAA 647235 0.6394012 6.434523 110-119
TCAAT 745850 0.63777775 8.804156 110-119
GACGA 1234000 0.6323461 9.739646 220-229
TAGCT 886865 0.6300386 12.927498 50-59
GCGAC 1419630 0.62968194 7.044928 70-79
ACTTC 850220 0.62929696 30.73373 4
AGTAA 816585 0.62448746 5.1112223 50-59
AGATG 977515 0.62106574 5.3912983 120-129
CCGTA 1078030 0.61768365 6.328418 230-239
TACTT 672445 0.6171 10.6152525 1
TCCAC 1028990 0.61427236 10.264842 180-189
CACCG 1326320 0.6129274 9.355048 45-49
AAGCA 981045 0.6051134 6.058745 120-129
GGCCC 1561295 0.5994286 10.366219 70-79
TGATT 680480 0.59937626 6.3576813 50-59
CACGA 1117735 0.5967509 8.752243 180-189
TAATA 602475 0.59518296 8.876498 130-139
CCCTA 991160 0.5916891 11.552124 110-119
CTTTG 764245 0.5826707 7.1920924 45-49
AATGG 915710 0.58179784 6.4899745 120-129
ACGAC 1085995 0.57980514 9.1600895 220-229
ACCCG 1254395 0.5796889 8.776196 140-149
GAAGA 970080 0.5743026 6.1825247 15-19
GGGGA 1403595 0.5735333 9.151859 4
CGGCA 1286715 0.570727 11.460264 70-79
GGTGA 1080590 0.57038337 6.0571265 120-129
TCAGG 1032615 0.5678831 5.522408 150-159
GTCCT 912015 0.560813 6.7638693 45-49
ACCTA 813060 0.5607457 6.9577646 130-139
TTAGC 789270 0.56070614 9.641696 45-49
ATTTC 609515 0.5593494 6.3523154 240-247
CTGCG 1163170 0.5536944 12.291515 25-29
CGGTT 928975 0.54828393 11.167276 160-169
GCTAC 942775 0.54018587 8.043021 50-59
GTTCC 877195 0.53940165 6.321343 140-149
TTCAA 629905 0.538633 5.288832 200-209
AAATC 675485 0.53821117 8.112338 110-119
ACCCC 1110155 0.5345139 9.512594 140-149
AAGAT 693765 0.53056026 5.704299 15-19
CCACG 1144625 0.5289614 7.6930766 180-189
GGAGG 1289315 0.5268364 5.7860336 6
AAGTA 688100 0.52622795 5.3392744 120-129
CTGTT 666755 0.508343 5.5251703 240-247
TATGT 575930 0.50728714 6.3132133 140-149
GGGTA 946920 0.49982643 5.455118 100-109
TAAAT 505610 0.49949035 8.802326 130-139
AGTTT 565770 0.49833807 5.0105233 140-149
TGACG 896195 0.49285945 8.723711 220-229
GGGTT 865240 0.49014372 5.595941 100-109
CGCGG 1325125 0.48830894 6.6758175 220-229
ACATT 566620 0.48451787 5.8412795 110-119
GTAAC 714550 0.4730003 6.5304527 120-129
TGGCC 980965 0.46696088 15.213331 2
TATCC 628775 0.4653927 5.2947316 110-119
CAATC 668525 0.46106383 6.6125555 110-119
AACAT 575220 0.45832226 5.564373 110-119
CCGGT 959545 0.45676455 8.617712 160-169
CCGCG 1185835 0.45527816 6.920657 220-229
ATGCT 638325 0.4534731 7.07977 50-59
TGCTA 629215 0.44700128 6.2434034 50-59
TGCGC 931220 0.44328123 9.124396 25-29
GGTTT 600215 0.4392207 5.104847 100-109
ATTCT 466920 0.42849055 6.7764964 2
GCGCT 897670 0.4273106 8.320234 25-29
CATAA 535985 0.4270607 5.719636 110-119
GCCTT 693050 0.42616782 9.010402 1
GCATG 774710 0.4260491 8.491502 60-69
TTGGT 579455 0.4240291 5.062367 120-129
GGTCA 763940 0.42012626 5.340411 160-169
ATGGC 748920 0.41186598 7.3824472 1
CACTT 546200 0.40427417 6.9745145 3
ACGAG 785735 0.4026389 7.6797814 180-189
CATGC 695030 0.39823443 7.293766 60-69
AGCTT 553350 0.39310595 7.2197366 1
CCGGG 1065185 0.39252105 5.279029 80-89
CTAAC 563155 0.38839293 5.241125 110-119
TACCT 520215 0.38504118 5.2543144 2
TTTGA 435345 0.38345793 6.153793 45-49
CCTAA 547540 0.37762374 7.244318 110-119
GAGGC 869955 0.37036356 5.7248783 35-39
GGTAA 579240 0.36802104 6.7673507 120-129
TTCTT 368255 0.36268398 20.004648 1
TGGGC 761400 0.34787658 8.882999 1
CGAGC 767430 0.34039629 6.5664477 180-189
TGCTG 569040 0.33584917 13.500992 1
AACCT 448735 0.30948052 5.101239 2
CGGGA 702710 0.29916278 5.071863 80-89
AACTT 347760 0.29737025 10.812527 3
CTCTT 374015 0.29709423 9.727035 1
CGCTT 479010 0.2945511 6.0289626 1
GCGAG 691715 0.2944819 5.271381 240-247
GACTT 405265 0.28790474 7.182001 1
TCACA 411400 0.28373158 5.108356 5
ATTGT 314110 0.27667245 5.172596 240-247
TTCCT 347530 0.2760562 6.36139 4
GAGGT 515045 0.27186364 7.335577 7
GTCTT 341320 0.26022696 12.664445 1
TAACT 304080 0.26001936 7.093802 2
CCCTT 405735 0.25994006 5.745745 3
TTGGC 438890 0.25903425 8.209373 1
TCGGT 429770 0.25365153 5.748094 7
TGTAA 305250 0.25052953 7.6233945 25-29
AATCT 290855 0.24871068 7.9778876 2
CGGGG 696350 0.24629228 9.087068 3
CCTCG 472015 0.23409788 5.3852606 5
TGCTT 282030 0.21502347 10.468157 1
TGCCT 312285 0.19202918 5.081747 4
TCGCA 295335 0.16921937 7.2655745 7
TAGGC 285940 0.15725173 6.8973947 1
TTGCT 163700 0.12480709 6.3901525 4
Produced by FastQC (version 0.10.1)