FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_13_SL248342.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_13_SL248342.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11123587
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12688720 8.619348 60.107132 110-119
CACAG 17161825 7.4734883 41.200348 110-119
ATACA 11641700 7.465699 36.550694 110-119
TCCCT 19557555 6.856173 101.184875 30-34
CAGTA 14011490 6.463187 38.453453 110-119
AGTAA 11033490 6.3451 46.6995 110-119
GGAGA 17570265 6.152918 94.46675 35-39
GGTGA 19653760 5.847738 101.44061 45-49
GTAAT 11966730 5.8470993 38.758038 110-119
CCCTG 19183700 5.6933584 66.2519 30-34
AATAC 8471025 5.4323783 38.54958 110-119
AGACG 13859225 5.412156 102.393265 40-44
GTAGT 15164970 5.329849 32.16123 80-89
ACAGT 11391845 5.2548037 35.005764 110-119
GTGAC 15179495 5.0364914 84.71254 45-49
TAGTA 9871680 4.8234305 41.59443 80-89
CCCCA 12315870 4.7972283 101.64094 60-69
GAGAC 12230165 4.7759933 142.43864 40-44
CTCAG 12549835 4.643413 34.055847 140-149
ACTCC 10880670 4.48935 114.31029 30-34
GGCCC 17757300 4.461512 58.129364 60-69
GTGTC 15337765 4.3238664 23.243378 120-129
GCACA 9921245 4.3204207 33.71552 110-119
ACCAG 9900305 4.311302 88.53329 45-49
AGTAG 10390990 4.2982416 33.593403 80-89
TGACC 11586435 4.286957 145.21994 45-49
CGTGT 15072860 4.249187 22.67089 120-129
CACTC 10005730 4.1283507 93.45907 25-29
CTCCC 12228505 4.047045 65.61981 30-34
CCCAG 11451400 3.9999573 86.94748 60-69
CCCTT 10982340 3.850012 41.871647 20-24
GACCA 8799655 3.8320003 102.377396 45-49
ATGCA 8300105 3.828653 91.571945 25-29
TGAGG 12647200 3.7630208 68.96044 35-39
AGGAG 10566415 3.7002459 94.68187 35-39
GACGG 13146935 3.6928713 109.00966 40-44
TACAC 7169360 3.6878338 26.457462 110-119
ATGGT 10460325 3.676364 25.144707 190-199
ACGGT 11016470 3.6552174 96.7495 40-44
AGATA 6308225 3.6277113 19.00825 150-159
TGTCC 11504160 3.616543 20.078884 120-129
CTGAG 10837025 3.5956783 104.05816 35-39
CCACT 8713185 3.5950487 26.438433 240-247
CAGGG 12526680 3.5186467 33.50522 50-59
TGGTG 13872270 3.506947 19.126451 25-29
GAGGA 9990040 3.4984055 124.75488 35-39
GCCCC 12383300 3.4695244 73.04114 60-69
CTTGG 12256380 3.4551935 24.048433 20-24
AGGCT 10267810 3.4068155 29.675644 30-34
CCGTG 12722495 3.3859453 22.857386 120-129
TGCAC 9146680 3.3842528 110.0454 25-29
TCTTG 9837430 3.2758415 17.587843 170-179
GGATG 10976915 3.2660477 66.94948 20-24
GGGTT 12912785 3.2643864 39.427006 50-59
CAGAT 7066845 3.2597775 17.508478 150-159
CTCTC 9245005 3.2409651 13.464923 100-109
CCTGA 8750625 3.237713 80.494835 30-34
TCAGG 9679670 3.211673 20.45151 140-149
CTTCA 7232610 3.1610065 16.101799 200-209
CCAGG 10033680 3.1428852 38.006252 50-59
GCACT 8411585 3.1122692 85.03416 25-29
TCGCA 8403215 3.109172 23.3276 100-109
TCTCA 7055690 3.0836837 21.124994 130-139
ACAGA 5678610 3.0829396 14.069438 140-149
TGTCT 9234910 3.075204 19.191786 180-189
CCTGG 11523210 3.0667696 27.793926 50-59
CGCAC 8680120 3.0319529 23.884956 100-109
GCCCT 10045435 2.9812944 22.524609 20-24
GGTTG 11550020 2.9198759 70.301605 15-19
CGGAT 8761900 2.907161 75.83189 20-24
GATGG 9663150 2.8751528 23.235607 15-19
TGGCC 10733850 2.8566902 42.355186 60-69
GACTC 7702740 2.8499978 18.867165 190-199
TTCCC 8067670 2.828234 46.511112 50-59
ACCAC 5791790 2.8125558 18.076044 230-239
TCTCT 7568005 2.81029 14.666398 180-189
GATGC 8409315 2.7901745 91.64253 25-29
TCAAA 4337165 2.781378 55.75918 70-79
GGTTC 9861210 2.7799718 44.472454 50-59
CGGTG 11591110 2.766331 68.308945 40-44
AGGGT 9270830 2.758423 47.095955 50-59
TCACA 5309355 2.731069 11.421777 140-149
GCCGT 10205185 2.7159922 21.582882 120-129
TCCAT 6178060 2.7001164 20.84009 70-79
CGGCC 10746375 2.7000208 12.359998 120-129
GTTGG 10650020 2.6923535 50.604893 15-19
TTCTT 6822480 2.68358 19.948132 170-179
AGATG 6373065 2.6362236 16.756605 180-189
TGGAG 8769040 2.6091213 20.305897 25-29
AGTCA 5639875 2.6015484 29.08281 70-79
GGCTG 10889150 2.5988014 15.433193 30-34
GTTCC 8059535 2.533662 50.330524 50-59
AGGGA 7202840 2.5223577 8.856488 150-159
CCAGT 6817040 2.522291 33.397514 60-69
TCCTC 7071565 2.4790356 16.51237 130-139
TGTTC 7329735 2.440785 12.553072 140-149
ACTCT 5569560 2.4341717 13.375295 200-209
GAGAT 5828905 2.4111314 16.30933 180-189
AATAA 3011820 2.407943 10.532349 110-119
GCGGA 8544280 2.4000218 83.37158 20-24
ACAGC 5477515 2.3853023 11.048325 120-129
TGTAG 6712245 2.3590717 7.731244 210-219
GGCGG 11633080 2.3504052 40.309944 20-24
CTGGA 7024065 2.3305545 14.22227 180-189
GTTCT 6953245 2.3154147 16.794762 160-169
CACGG 7351920 2.302868 9.5411005 120-129
TACCA 4449030 2.2885282 15.199491 230-239
CAGCT 6134205 2.269643 11.847934 150-159
TTGGG 8917800 2.2544436 76.08035 15-19
CAGAG 5759670 2.2492046 9.060817 140-149
CATTT 4829795 2.2359474 17.258715 150-159
TCATT 4797065 2.2207952 16.095598 150-159
TTCAT 4777580 2.2117746 15.467006 140-149
CTGGC 8303125 2.2097807 32.654663 60-69
GTGAA 5309895 2.1964424 17.44074 190-199
TTGTC 6523600 2.1723437 12.871214 170-179
TTCAC 4951030 2.1638439 17.800947 200-209
ACTAC 4173075 2.1465805 12.063965 230-239
GATAC 4648620 2.1443045 14.1353445 150-159
TCAGC 5781485 2.1391375 13.837618 140-149
ATGTA 4371800 2.1361182 9.342655 70-79
CTACT 4883625 2.1343844 7.4767947 230-239
ATTTG 5064015 2.1023226 15.1942625 150-159
GAATC 4525735 2.0876205 14.436278 190-199
CTCGC 7030065 2.08639 16.066744 100-109
CACTA 4035040 2.0755765 14.212469 240-247
CTCTG 6572395 2.0661523 14.097175 180-189
TCTGC 6558360 2.0617402 11.573465 210-219
GCTGT 7284725 2.0536354 9.846204 140-149
TGAAT 4170240 2.0376332 13.899856 190-199
GTCCT 6453445 2.0287585 14.060373 130-139
GAACT 4394620 2.02714 10.436833 160-169
ACACA 3347730 2.0267582 13.523447 110-119
TTGGA 5756890 2.0233045 15.003186 170-179
TCTCG 6421810 2.0188131 15.589405 100-109
TACAG 4375920 2.0185142 13.229971 160-169
GTCTC 6413460 2.0161884 11.497823 180-189
CCAGA 4617365 2.0107317 31.49806 60-69
ATCTC 4599875 2.010372 7.9154234 100-109
TGGGG 9366775 2.0046597 50.161747 15-19
CAGGC 6380790 1.9986774 15.044246 140-149
AATCG 4327125 1.9960063 13.211496 190-199
AGCTC 5384945 1.9924185 11.803719 140-149
GTTCA 5067845 1.98621 14.526564 140-149
TTTGC 5933720 1.975915 11.651952 150-159
ATATA 2897790 1.968446 10.184891 110-119
GATAT 4020280 1.9643608 7.8892183 180-189
ACGGC 6246645 1.9566586 14.157327 120-129
TGGAA 4727295 1.9554495 8.596498 170-179
TTGCA 4978240 1.9510916 13.737661 150-159
TAGTC 4972885 1.9489928 23.708155 70-79
AAAAA 2052370 1.9312274 8.131199 8
ACACG 4424095 1.9265679 13.336594 120-129
AATAT 2832740 1.9242581 12.83459 110-119
GTCAA 4162890 1.9202482 37.328705 70-79
AACCA 3158530 1.9122142 14.397164 70-79
CCTTG 6082590 1.9121732 27.285818 20-24
GACGT 5744010 1.9058378 10.025974 60-69
GAGTC 5739305 1.904277 11.802001 210-219
ATATG 3888760 1.9000984 8.123758 180-189
CCTTC 5417130 1.8990504 13.226424 200-209
CAGCC 5404965 1.8879461 10.065021 120-129
TACTA 3450595 1.8801283 10.015322 240-247
CATAG 4068225 1.8765814 8.20894 210-219
TCGGC 7033865 1.8719819 7.4552283 130-139
GTTGT 6036765 1.8026732 6.724091 210-219
AGTAT 3664645 1.790593 11.409457 220-229
CTGTT 5370220 1.788271 12.22848 140-149
GGGGC 8824385 1.7829226 45.693996 15-19
CGTAG 5314685 1.7633896 9.359094 210-219
GTCCC 5903810 1.7521389 23.679886 50-59
AGAAC 3221395 1.7489082 11.091089 160-169
GGGCG 8588330 1.7352288 56.25861 20-24
GCAGA 4409140 1.72181 13.973959 150-159
ATTGT 4130525 1.7147847 8.563527 50-59
CTCCA 4138125 1.707385 7.7377367 150-159
GGTCA 5143630 1.7066343 7.4140954 130-139
TGCAG 5113895 1.6967684 12.978272 150-159
TTGGT 5671665 1.6936486 28.806608 25-29
CAAAA 2239335 1.6901829 22.463526 70-79
CACCA 3477735 1.688826 12.7167225 230-239
GTCAC 4552065 1.6842546 8.351454 140-149
TGACT 4295235 1.6834056 11.441573 190-199
TCCCA 4078790 1.6829033 10.951045 240-247
TCAGA 3644540 1.6811448 10.327867 130-139
ATAGT 3437805 1.679756 7.2702346 220-229
AGTCT 4271855 1.6742425 12.39388 210-219
CAGAC 3772030 1.6426121 19.216805 60-69
CTGTG 5811510 1.6383216 5.3725815 210-219
AACTG 3524460 1.6257547 10.272375 160-169
GAGAA 3331045 1.6217167 10.350989 160-169
AATAG 2819200 1.6212554 9.11603 240-247
TGGTT 5395125 1.6110694 17.3403 10-14
ATAGA 2774315 1.5954431 9.909921 240-247
TGTGC 5656210 1.5945411 7.8407054 220-229
GCTCC 5349480 1.5876242 6.2380652 150-159
TTCAG 4042470 1.5843409 9.059928 150-159
TGCTC 5026730 1.5802444 5.371947 150-159
ACCAT 3065640 1.5769287 13.128673 45-49
TCTGG 5583360 1.5740038 11.478874 180-189
GCTGA 4714915 1.5643885 25.295113 30-34
TTGTA 3767610 1.5641209 8.563802 210-219
ACTGA 3380605 1.5593976 8.275544 180-189
CTGGT 5526965 1.5581057 6.7141514 160-169
GTCTG 5522135 1.5567441 8.530089 210-219
GGACG 5512190 1.5483311 7.0046253 170-179
GGCCG 6870600 1.548001 12.708157 120-129
TAGTT 3702355 1.5370302 9.29835 220-229
GCTCA 4150020 1.5354989 10.75917 140-149
GTGCT 5439630 1.533485 7.4530234 220-229
CCCAC 3920700 1.5271754 10.346332 240-247
GAACC 3491430 1.5204188 11.898816 70-79
CATGT 3871820 1.517459 9.351122 70-79
CCACC 3859590 1.503372 9.120085 230-239
CCTCG 4945680 1.467784 9.28873 130-139
CAAAG 2701345 1.4665707 26.463669 70-79
GTGGA 4909215 1.4606773 25.747581 25-29
ACCGT 3942795 1.458826 22.56189 45-49
GCTCT 4575285 1.4383245 9.480305 130-139
CGACT 3869060 1.4315442 8.296566 190-199
TCGAC 3800765 1.4062752 8.923137 190-199
CCTCA 3394645 1.4006261 7.398958 130-139
CTCTT 3753845 1.3939464 7.887128 200-209
CACTG 3766990 1.3937783 6.980286 230-239
GTCCA 3764140 1.392724 19.571035 70-79
TGATA 2840835 1.3880688 7.2677207 180-189
TGGAC 4175320 1.3853532 10.02415 170-179
CCATG 3737050 1.3827008 9.526924 70-79
GTAGG 4587890 1.3650709 5.2291093 160-169
GGGAG 5357250 1.3494385 6.3532443 150-159
CCATC 3265775 1.3474544 10.693229 240-247
GGAGG 5338160 1.34463 19.451271 30-34
GCGTA 4050025 1.3437809 9.895524 210-219
ACTTC 3072615 1.3428841 6.6373906 220-229
GAGGG 5329260 1.3423883 7.79971 200-209
TATAC 2458530 1.339581 6.439076 110-119
AATTG 2733600 1.3356724 8.55625 170-179
AGATC 2882330 1.3295544 6.9333987 130-139
CTCGA 3559370 1.3169595 7.974851 190-199
TGGTC 4671155 1.3168445 14.559675 50-59
GTGGT 5207040 1.3163536 12.904791 50-59
TATGG 3744120 1.3159006 6.000555 180-189
CTGCT 4182490 1.3148422 5.352811 90-99
ACGGA 3342245 1.3051776 8.891626 200-209
TCCAC 3151525 1.300315 9.27062 240-247
CTTGA 3310885 1.297615 8.080768 200-209
TTGGC 4597585 1.2961043 9.366993 60-69
CTGAT 3286850 1.2881953 6.970274 180-189
AGCCG 4064450 1.2731221 8.990918 120-129
GTGTT 4251460 1.269553 7.218239 160-169
TGCCC 4258470 1.2638332 8.301777 60-69
ACGTG 3790270 1.2575953 8.965944 170-179
ATCGG 3787555 1.2566944 7.848459 190-199
ATAAA 1565555 1.2516576 5.757732 7
GAATT 2556020 1.2489045 9.486636 170-179
ACTAT 2265190 1.2342358 6.2403603 230-239
GGAGT 4142495 1.2325491 6.573459 210-219
AAGTA 2124595 1.2218045 15.053906 70-79
ACTCG 3292940 1.2183808 8.180189 190-199
GGCTC 4576255 1.2179174 7.8885374 130-139
GATCT 3105200 1.2170024 5.518129 130-139
ATAAC 1881445 1.2065507 7.0393643 80-89
GCATA 2597525 1.1981802 7.7935586 210-219
TTGAG 3397640 1.1941276 7.7814937 200-209
CCATA 2315675 1.1911558 11.878901 70-79
ATACC 2300220 1.183206 8.603916 70-79
ATGGG 3959650 1.1781458 36.529053 15-19
ACTGG 3545235 1.1762937 6.818105 160-169
GGAAT 2831365 1.1711963 7.962199 170-179
ACTGT 2978185 1.167222 5.390561 230-239
AAAAG 1709660 1.1571665 15.430764 70-79
TTCCA 2646895 1.1568234 7.22825 230-239
TATCA 2114430 1.152091 7.4091964 70-79
TCTGT 3445920 1.1474833 6.4754515 210-219
AGAGA 2347620 1.1429371 14.793628 35-39
CAATA 1778000 1.1402125 6.683949 100-109
AAAGT 1976680 1.136742 19.202457 70-79
AGTTC 2898095 1.1358329 5.4669585 160-169
AGCTT 2872245 1.1257017 5.910559 150-159
GAAGA 2285855 1.1128668 19.99402 35-39
TCACG 2991820 1.1069671 8.919347 200-209
AGAGC 2826435 1.1037492 6.6791577 140-149
AGGTT 3084310 1.0840052 22.376663 10-14
TCTAC 2477445 1.0827655 6.9547644 180-189
ATATC 1985665 1.0819308 7.919906 70-79
AACTT 1984610 1.0813559 6.034039 200-209
GTCTA 2753460 1.079147 7.172826 180-189
ATCCA 2096800 1.078569 14.939338 240-247
CTGAA 2333820 1.076539 16.168913 35-39
CTGCC 3590950 1.0657259 5.860453 200-209
CTCGG 3996730 1.0636836 7.9016523 130-139
CGGCT 3981165 1.0595411 7.4303164 130-139
TACTG 2663170 1.0437602 6.035199 180-189
GTCCG 3914155 1.0417073 5.043269 130-139
TATGT 2508665 1.0414706 7.0901647 220-229
CATGG 3115230 1.0336199 5.353384 190-199
TCCCC 3121215 1.0329716 5.3432364 80-89
GAGCT 3106205 1.0306256 5.958652 140-149
GCTTC 3271760 1.0285375 5.2201085 150-159
GCGGT 4287115 1.0231616 5.6656284 130-139
CGTGG 4275060 1.0202845 8.904157 50-59
TAGAT 2082400 1.0174876 7.52898 240-247
CTGCG 3816235 1.0156469 6.4679894 210-219
AAAAT 1252520 1.0013868 5.2723484 8
CTTCT 2695335 1.0008811 5.404072 210-219
TATAA 1459495 0.9914235 6.3943086 240-247
ACGTC 2674105 0.9894133 13.484155 70-79
CTACC 2380945 0.98237467 7.7521353 230-239
ACAAT 1523145 0.9767767 7.720223 100-109
TGCGT 3454795 0.9739406 7.809556 210-219
GGTGG 4547970 0.9733481 25.559727 25-29
CCATT 2222505 0.9713441 6.4368258 50-59
AAGAG 1983015 0.9654293 14.882247 35-39
GGGGT 4506505 0.96447384 7.1965623 10-14
GAGGC 3430050 0.96347445 16.474426 30-34
CGAAC 2207345 0.9612362 12.531721 70-79
TAGGC 2893620 0.9600907 5.852648 160-169
AGCTG 2860890 0.949231 5.1818132 30-34
GAGTT 2700240 0.9490208 5.2481174 160-169
CCCAA 1953525 0.9486531 9.324273 240-247
TGAAG 2287815 0.94635653 10.713878 35-39
ACCCA 1920930 0.93282455 12.208701 240-247
CGGAG 3307075 0.92893165 6.1976967 210-219
CAGCG 2929265 0.917544 5.814262 160-169
GGGCC 4066850 0.91629374 19.399101 15-19
TGCAT 2335585 0.9153717 5.944537 210-219
TGTAA 1865360 0.9114391 6.310598 230-239
ATCAA 1413210 0.90627664 14.242198 70-79
ACTAA 1389085 0.89080554 11.598385 240-247
CGTCC 2998780 0.88998103 10.999355 70-79
CATCA 1725965 0.8878159 5.5389433 230-239
TCGAA 1922715 0.88690543 16.458986 70-79
TTCTG 2656885 0.8847366 5.3720984 210-219
GACCG 2789625 0.8738041 20.753271 45-49
TGGGC 3657420 0.87287873 21.019009 15-19
CCCCC 2758285 0.86178976 5.0727468 80-89
CCAAT 1670555 0.8593137 10.157108 240-247
GGTCC 3222970 0.85775626 14.713855 50-59
CGGTC 3166750 0.84279394 5.371669 130-139
CCAGC 2356450 0.8231045 5.4397273 80-89
CTTCC 2286550 0.8015819 5.0752544 230-239
TGAAC 1735865 0.80071574 5.95519 190-199
AGCGT 2400320 0.79641587 5.9234123 160-169
AAAGA 1152290 0.7799161 5.85878 8
CATAC 1503965 0.7736218 10.294005 70-79
TAACC 1501410 0.7723075 5.6129065 80-89
TGAGA 1854345 0.76705134 5.413195 240-247
AACCG 1736240 0.7560833 5.280383 190-199
CCTTT 2011965 0.74711967 5.6025295 50-59
AAAGG 1510895 0.73557806 8.781377 10-14
AATCC 1424095 0.7325376 8.970148 240-247
ATGAT 1485490 0.72582966 6.657547 240-247
AGCCA 1659035 0.7224627 5.5663533 80-89
AAAGC 1320000 0.7166332 10.47467 70-79
GTCGA 2152805 0.71429145 11.368684 70-79
CTAAT 1310015 0.71378887 8.95665 240-247
GTAGC 2145170 0.7117582 5.4004993 80-89
CATTG 1787635 0.700617 5.8905115 50-59
CGATG 2089400 0.69325393 16.110933 15-19
AGGAT 1653365 0.6839158 6.9691563 10-14
TAGGT 1927740 0.67751944 5.294427 10-14
AAGGT 1637445 0.67733043 9.53853 10-14
GGAAG 1926310 0.674573 7.0574784 30-34
CCGTA 1769250 0.65461886 5.500132 80-89
GAGGT 2190810 0.651849 6.5581217 10-14
AGAAG 1327550 0.64631665 5.5263424 9
TATGA 1304785 0.63753486 5.5735183 240-247
CAATC 1232775 0.6341248 8.697015 240-247
ATAGG 1515530 0.6269002 5.2682953 10-14
CATCC 1449035 0.5978699 7.3725133 240-247
TAAAA 735325 0.5878906 6.026811 8
AATGG 1397180 0.57794464 6.5043 10-14
AAGAA 837965 0.56716835 5.367204 8
AAATG 980485 0.5638538 5.259339 9
GGGTC 2341540 0.5588312 5.35779 70-79
AAGCA 1016710 0.5519759 7.1050224 70-79
GAAAA 809050 0.5475975 5.916465 8
GAAGG 1535675 0.5377769 5.4861836 10-14
TTGCC 1639100 0.515281 5.8234906 60-69
ACGAT 1110785 0.5123803 7.114995 10-14
AAGGG 1446080 0.50640166 6.08021 10-14
TGATC 1270490 0.49793547 5.16082 240-247
ATAAG 845390 0.48616385 5.6584945 9
GGTCG 2010065 0.47972155 5.452103 70-79
GATCC 1289060 0.47694954 5.189503 240-247
GAAGC 1174215 0.45854187 5.6698623 30-34
TACCG 1211995 0.4484356 5.4530425 70-79
AAGCT 958610 0.44218537 9.50864 30-34
CGGTT 1558305 0.43930146 7.7690754 10-14
TAAGG 1056360 0.43696418 5.787503 10-14
TAAAG 691705 0.39778322 5.580368 9
Produced by FastQC (version 0.10.1)