FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_10_SL248339.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_10_SL248339.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7792061
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36420 0.46739880501448844 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30190 0.3874456321633006 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28009 0.35945560487783657 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26911 0.3453643394218808 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24781 0.31802882446633823 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24549 0.3150514350439505 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24454 0.3138322454097831 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 23272 0.2986629596457214 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 22677 0.29102698246330466 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21593 0.27711538705869987 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21524 0.27622987037704144 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21189 0.27193062271971435 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20277 0.2602264022317074 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20099 0.2579420258645306 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19862 0.2549004685666603 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19657 0.25226958567187807 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19594 0.25146107044079863 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19111 0.24526245366918972 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19020 0.2440945983354083 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18737 0.2404626965830991 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17841 0.22896381329663615 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17338 0.2225085250230972 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17300 0.2220208491694303 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17238 0.2212251675134473 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16496 0.21170265479184522 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16409 0.21058613375844978 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16343 0.20973911780208088 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16184 0.20769857936173752 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16118 0.20685156340536862 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15920 0.20431051553626184 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15905 0.20411801190981438 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15402 0.19766272363627543 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15380 0.19738038498415245 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15334 0.19679004052971352 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14996 0.1924522921470969 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 14795 0.18987274355270062 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14591 0.18725469423301486 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14545 0.1866643497785759 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14466 0.1856504973459525 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14161 0.18173625694152035 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13740 0.1763333218258943 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13346 0.17127689323787376 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 13320 0.1709432202853648 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12866 0.16511677719155432 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 12815 0.1644622648616329 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12542 0.16095869886028868 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12278 0.157570635034813 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12173 0.1562231096496806 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12169 0.15617177534929463 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12032 0.15441357556107427 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11925 0.15304038302574888 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 11764 0.15097417743521258 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11685 0.14996032500258916 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11615 0.14906197474583424 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 11483 0.1473679428330964 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 11301 0.1450322321655336 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11029 0.14154149973928593 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10866 0.13944962699855662 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10628 0.13639523612558987 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10543 0.13530438224238747 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10050 0.12897742971981352 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9964 0.12787374226151463 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9678 0.12420333978391597 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9678 0.12420333978391597 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9190 0.11794055513682453 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9146 0.11737587783257858 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9053 0.11618235534860417 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 8758 0.11239645069513701 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 8048 0.10328461237662281 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 15640050 7.591837 64.46719 35-39
TATTA 7740230 7.146991 123.909485 240-247
TGGAT 11833185 7.096131 76.41527 35-39
TCACC 12117510 6.4078026 73.92462 160-169
TCTCC 10604320 6.055342 84.90019 20-24
TCCCT 10367290 5.9199924 74.901695 15-19
CTGTG 11282440 5.913872 85.24363 25-29
AGTGG 12236365 5.6907244 39.663403 100-109
CTGAG 11284300 5.4775124 49.249096 15-19
TGTAT 7065660 5.4635816 58.84028 240-247
TTACT 6592510 5.3207035 110.99454 240-247
ATTAC 6567115 4.908318 103.98689 240-247
TGGGT 9632915 4.8376346 44.765156 100-109
CCTGA 9427680 4.7764697 50.83194 15-19
CTCTG 8647865 4.7311997 73.888466 35-39
ACACG 9727085 4.563781 48.237076 230-239
TTCAC 6949475 4.5400333 72.53891 40-44
GACAC 9670780 4.5373635 45.180138 230-239
CTCCA 8557980 4.5255046 40.2709 80-89
TACTG 6868250 4.298922 78.33815 240-247
TCTGG 8178135 4.2867002 134.56926 35-39
TGAGA 7688420 4.2696953 90.42916 15-19
CCCTG 9531530 4.2209845 85.578094 10-14
AAGGG 9756430 4.201902 38.8547 90-99
ATTCA 5580490 4.170906 108.03162 40-44
TGGAG 8916195 4.1466246 38.61161 90-99
CAGCC 9934690 4.074228 54.473083 30-34
TCAGT 6460760 4.0438695 91.6201 7
GAAGG 9384975 4.041924 30.767069 90-99
CCAGG 10278670 4.0386324 52.94818 80-89
AGCCT 7968070 4.0369687 49.73928 30-34
ACCAT 6589825 3.9867659 66.45486 160-169
ACTAC 6547900 3.9614017 35.8472 130-139
GAGAC 8801965 3.9566548 89.63117 8
CACCA 8038915 3.9367063 56.670063 160-169
GTATT 5049550 3.9046075 68.892784 240-247
GGGTC 9481680 3.8543348 121.240326 8
TACTA 5138230 3.8403573 27.674675 130-139
TCCAG 7579890 3.8402994 39.552036 80-89
CACCT 7155050 3.7836277 45.864643 20-24
ACTGT 5981820 3.7440948 67.828964 240-247
CTGGG 9101300 3.6997092 128.1159 3
GACTC 7219595 3.6577587 51.06154 20-24
TGAAG 6551990 3.638589 37.969677 150-159
GATTC 5775440 3.6149192 58.06531 40-44
CAAGA 6716190 3.6050901 43.710594 180-189
AGACT 6164665 3.573245 66.005486 15-19
GCTGG 8790035 3.5731788 33.04529 90-99
CCTGT 6475275 3.5425875 61.447685 25-29
GGACA 7819895 3.5151956 27.519392 220-229
ACTGG 7217955 3.5036674 44.043274 60-69
GAGTG 7501850 3.48886 38.71155 100-109
ACTCT 5118285 3.3437324 67.07641 20-24
TCCTG 5988065 3.2760377 62.123257 20-24
TATCT 4055015 3.2727342 47.327187 200-209
AGCTG 6726650 3.265183 37.222153 60-69
GGATT 5415165 3.2473693 55.346157 40-44
AGACA 5989185 3.2148511 37.611042 170-179
CTCCT 5621355 3.2099402 91.52034 20-24
GGTCC 7550635 3.2036211 138.77797 9
CTGCA 6244555 3.1637614 46.423332 25-29
CATCT 4753370 3.1053364 32.08218 170-179
CCATC 5872185 3.105242 36.144615 45-49
GTCTC 5668915 3.1014328 34.10942 30-34
GTCCC 6981675 3.0917957 73.55737 10-14
CCTCA 5836425 3.086332 78.08808 5
AAAAA 4123795 3.0240293 19.827042 90-99
GGAGT 6442535 2.9962084 36.97653 90-99
GTGTA 4989360 2.992022 36.103184 230-239
AGGGA 6872480 2.959842 39.878162 80-89
AAGAA 4736755 2.9088757 37.194454 180-189
GGCTG 7146955 2.9052613 34.088608 90-99
GAGCT 5923500 2.875326 30.513458 60-69
ATCTC 4355790 2.8456004 47.010548 170-179
AGTAG 5102610 2.833689 28.383602 50-59
GGAAG 6528780 2.8118172 29.92967 80-89
TGTGC 5343695 2.8009834 56.16895 25-29
CAGGG 7169570 2.6989636 36.190952 80-89
ATCTG 4307210 2.6959357 35.67402 200-209
GTGAA 4851315 2.6941342 37.16992 150-159
GGGAA 6237795 2.686496 33.49058 80-89
CTATG 4258070 2.6651783 27.343609 50-59
CCCTC 5745585 2.6556997 29.074703 20-24
ACCTT 4050135 2.6459186 68.93038 40-44
GACAA 4875345 2.6169686 23.714237 180-189
GAACA 4826165 2.59057 33.30764 210-219
GAGTC 5302740 2.574003 28.8764 150-159
CACGG 6427350 2.5253954 43.612328 230-239
AGAAC 4686520 2.515612 29.708767 190-199
ATCAG 4324700 2.50674 27.357464 45-49
CAGTG 5122895 2.4867043 71.12129 8
ATGAA 3740040 2.4801655 26.347437 210-219
CGCCA 6045710 2.4793527 39.330242 70-79
CTGTC 4529185 2.4778926 51.192047 30-34
CCAAG 5251335 2.463836 38.744747 180-189
TATAT 2654080 2.4506617 23.813116 240-247
CTACA 4042850 2.4458764 18.694607 190-199
TGCAA 4208230 2.4392304 34.21767 200-209
AGGGC 6477710 2.4385152 31.499535 150-159
TCTGT 3573740 2.415442 26.075596 210-219
ACGGC 6142200 2.413356 48.429134 230-239
GTGGA 5185800 2.4117424 27.24081 100-109
AGGCT 4924635 2.390467 19.689125 80-89
CCGCC 6663395 2.388556 33.918564 70-79
GTGGT 4750760 2.3858242 30.969704 120-129
GTATC 3790930 2.3727896 22.447002 200-209
CTACT 3621195 2.365696 23.248524 50-59
CACTG 4647370 2.3545582 20.466879 30-34
GCAGC 5979400 2.34939 33.97785 30-34
CTCTC 4078130 2.3287182 95.88095 20-24
TGGTG 4603090 2.3116646 35.542183 35-39
TCCAA 3804650 2.3017678 43.555756 180-189
TATCA 3077315 2.3000116 17.66574 170-179
TATGC 3655170 2.287816 20.83869 50-59
TGAAC 3922300 2.2734957 36.44095 210-219
GGGCT 5561005 2.2605672 41.98791 90-99
CTTCA 3445380 2.2508373 49.739277 45-49
ACAGC 4775155 2.2404206 25.99522 210-219
GGCCG 6781655 2.2314572 25.252886 230-239
CAGTA 3816510 2.2121763 21.328115 45-49
GCCTC 4993385 2.2112927 68.989105 4
GCTCC 4975395 2.2033257 33.547283 80-89
CAGGC 5600835 2.2006462 32.27592 70-79
CAAAT 3169255 2.1935883 33.397465 200-209
ACAAC 3884620 2.1763825 18.668812 180-189
CCTTC 3759760 2.1469207 55.629616 45-49
GCCTG 5055625 2.1450257 29.892574 210-219
GTGGG 5452915 2.12373 29.403399 100-109
AATGA 3198920 2.1213279 24.73018 210-219
GGGGG 7005325 2.115895 91.626656 5
AAATG 3176375 2.1063774 31.680908 200-209
TCTAT 2595875 2.0950868 20.928942 110-119
GCTGT 3988030 2.09039 19.223045 230-239
TACAT 2793660 2.0880055 17.259274 190-199
GCTAT 3307095 2.0699515 33.15162 50-59
CCTCT 3617850 2.0658865 48.475376 35-39
CTCAC 3905565 2.065283 58.00968 20-24
AGAGT 3706910 2.0585992 30.655748 150-159
GTAGT 3417620 2.0494802 23.895325 120-129
TGCAG 4210320 2.0437315 42.913357 30-34
GCAGA 4518545 2.0311744 23.520433 140-149
TGCAC 4003765 2.0284805 27.080957 25-29
TGTGA 3340445 2.0031998 20.159143 210-219
GGTGG 5141100 2.0022886 19.307348 40-44
AACTA 2887905 1.998853 36.74391 130-139
AGCAC 4217425 1.9787432 21.05672 130-139
ATATC 2645180 1.9770303 14.2934675 160-169
TCTCT 2792035 1.9696453 33.91106 35-39
GGCTC 4634915 1.9665248 20.813686 40-44
TGAGC 4049780 1.9658036 17.886116 200-209
AACAG 3661235 1.9652635 32.09518 210-219
TCCGC 4415555 1.9554037 41.68483 70-79
TGGTA 3232510 1.9384733 30.228071 120-129
CATAT 2570450 1.9211764 15.02444 160-169
CAGAG 4256615 1.9134314 31.11533 170-179
CCGTG 4499670 1.9091423 20.25941 230-239
ATGGA 3437550 1.9090126 11.866464 100-109
TTCAG 3036025 1.9002855 46.25033 45-49
GGTCT 3625155 1.9001832 34.661865 15-19
CAGAC 4042950 1.8968825 25.660748 140-149
ACCCT 3577930 1.8920281 45.497375 10-14
GGATC 3894405 1.8903832 18.546165 70-79
CTGTA 3005750 1.881336 23.583477 190-199
TGTCT 2778655 1.8780549 51.52025 30-34
ACCAG 3994880 1.874329 14.332088 190-199
CAGTT 2992800 1.8732305 25.787006 190-199
CCTGC 4229320 1.8729305 49.34733 20-24
TGGGG 4775275 1.8598117 119.012024 4
TCCAT 2845740 1.8590977 27.945417 45-49
GCCAG 4727505 1.8575028 35.123108 70-79
GTCAC 3665800 1.857253 38.037334 160-169
CTATT 2287030 1.8458233 20.203875 110-119
GCACA 3912610 1.8357294 18.112087 130-139
GCTGA 3769605 1.8298041 27.18082 200-209
CCAGA 3891030 1.8256042 32.700325 170-179
ACTAT 2440365 1.8239498 16.563328 130-139
AGAGA 3507545 1.8038602 26.674408 170-179
TGGGA 3864125 1.7970755 15.944035 100-109
GATGG 3852290 1.7915714 20.283218 100-109
ACCTG 3523180 1.7849951 32.437756 25-29
AGGAC 3951890 1.7764516 19.609026 220-229
TTCCA 2695930 1.7612283 22.715498 180-189
TACAA 2541685 1.7592179 18.371399 130-139
TCAGC 3463190 1.7546017 11.062538 45-49
TAGTG 2922600 1.7526263 26.069069 120-129
CACAG 3715015 1.7430211 15.24507 140-149
AGTCA 3007010 1.7429632 29.305397 160-169
GCCGA 4426360 1.7391789 18.915945 220-229
ATACT 2322545 1.7358902 25.793018 130-139
GAGGA 4021510 1.7319852 25.607635 220-229
AGTTC 2760205 1.7276464 23.142431 190-199
TGACC 3409275 1.727286 15.075921 210-219
CAAGG 3824120 1.7190164 16.399544 80-89
CTGAA 2960935 1.7162566 23.889647 200-209
GCCGT 4008860 1.7008991 29.521585 230-239
GGAGC 4490955 1.6906071 12.5859 200-209
CCTGG 3972305 1.685389 32.657963 2
GTGCA 3471920 1.6853046 48.78415 25-29
ATTGG 2795005 1.6761101 21.707024 100-109
TTCTG 2472000 1.670791 41.445705 30-34
CTCAG 3289295 1.6664988 74.41736 6
CATGA 2872670 1.6650951 22.391985 60-69
AGCTA 2861475 1.6586062 25.368475 50-59
ATGCA 2849920 1.6519086 16.176367 60-69
GCAAA 3075070 1.6506242 30.662613 200-209
TAGTA 2298050 1.6455992 15.548848 120-129
CTCCC 3532055 1.6325713 14.783387 190-199
AACAC 2878310 1.6125911 21.86677 190-199
GGATG 3449475 1.6042359 21.536774 100-109
GTTCT 2360525 1.5954466 24.716024 190-199
TCTCA 2435290 1.5909542 25.688143 100-109
TTCTC 2243790 1.582885 23.721703 190-199
AAGAG 3067410 1.5775076 19.738483 150-159
AGGGG 4372540 1.5770454 32.264523 90-99
CGTCC 3556425 1.5749428 20.03246 180-189
CAGCT 3107920 1.5746065 12.930675 50-59
TCTGC 2867740 1.5689249 32.591328 200-209
GGGGC 4955305 1.5621753 28.385015 90-99
GGGCC 4746430 1.5617805 27.03732 2
GCACT 3074235 1.5575405 26.105263 25-29
ACAAT 2203595 1.5252103 22.46683 180-189
CTGGT 2907105 1.5238057 32.17467 35-39
TCTGA 2428855 1.5202502 20.430962 220-229
CGATT 2426270 1.5186323 33.52601 160-169
AGTTA 2111045 1.5116878 16.041786 50-59
CGGCC 4372920 1.5018201 31.60304 230-239
CATCA 2474955 1.4973184 31.736723 45-49
CCATG 2952660 1.495945 15.310628 160-169
GTTAC 2378080 1.4884695 17.124432 50-59
CGTGT 2833855 1.4854106 24.170736 230-239
GGCTT 2801175 1.4682809 20.518797 25-29
GAGCA 3257855 1.4644693 7.1666336 200-209
GTCCG 3431675 1.4560081 33.54378 70-79
AGTGA 2612510 1.450834 81.04251 9
CTTTA 1788195 1.4432219 41.150314 45-49
GTGAC 2961555 1.4375683 16.134321 210-219
TTAGT 1848705 1.429527 24.284536 110-119
ACTCC 2702170 1.4289216 16.792076 140-149
CTCAA 2355485 1.4250404 18.930218 150-159
CAACC 2907270 1.4237081 15.713585 140-149
GTCCA 2800175 1.4186896 29.552418 180-189
AACCC 2868740 1.40484 20.8987 140-149
TACGC 2764900 1.4008178 23.806381 140-149
GGCAG 3697040 1.3917401 12.995632 150-159
CAACT 2282855 1.3811002 18.722057 130-139
AAGCT 2381040 1.38013 17.547485 200-209
TAGCA 2378810 1.3788376 11.782748 130-139
GGGGT 3512325 1.3679345 113.57704 7
ATCAT 1829995 1.367754 15.834079 110-119
TGTCC 2482915 1.3583893 57.10515 15-19
CACGT 2658005 1.34666 17.146954 180-189
GGCCT 3169900 1.3449409 65.91323 3
AGAGC 2988360 1.3433261 24.038536 220-229
CTACG 2646990 1.3410795 15.514489 130-139
GAGAG 3093365 1.3322513 24.780563 220-229
ATCTA 1776950 1.3281077 21.15736 110-119
TCAAG 2289745 1.3272125 23.96846 150-159
TCGGA 2732805 1.3265309 97.11489 5
TGTGT 2044355 1.3238428 14.9252615 240-247
GTGGC 3247625 1.3201703 15.117118 40-44
AGCAG 2921410 1.3132308 12.28326 50-59
ACAGT 2262265 1.3112841 12.422765 120-129
AACCA 2338295 1.3100445 17.6895 190-199
TGGAA 2358600 1.3098273 19.47124 120-129
CCATA 2164670 1.3095995 15.70398 160-169
AGATC 2257760 1.3086728 11.97108 210-219
CCAGT 2565570 1.2998285 19.7279 190-199
AGACC 2768835 1.2990898 102.9168 9
TTACA 1737795 1.2988429 9.936408 120-129
CAATT 1737365 1.2985215 23.884903 180-189
CCTTT 1837660 1.29638 48.32949 45-49
ATCCG 2553235 1.2935791 24.716228 70-79
CGACA 2756050 1.2930913 15.394462 70-79
GTGCG 3172830 1.289766 17.06412 240-247
GAACT 2210855 1.2814851 13.807515 190-199
ATACA 1840585 1.2739542 20.512749 35-39
ACGTC 2507165 1.270238 18.857248 180-189
GATTG 2115940 1.268888 24.731335 100-109
ACAGA 2357475 1.2654362 19.020245 140-149
ACATG 2180410 1.2638382 13.557888 190-199
TCACT 1933380 1.2630608 12.561184 190-199
TCCGT 2304830 1.2609599 13.8357115 150-159
GATCT 2009745 1.2579242 8.990001 220-229
TTATT 1260945 1.2572616 18.869135 240-247
AATTC 1680655 1.256136 23.802673 180-189
CCTAC 2354390 1.2450137 16.5873 190-199
ACATA 1794220 1.2418628 20.144016 130-139
TGCGA 2551870 1.2387033 14.197213 240-247
AGTCG 2535925 1.2309633 16.961632 150-159
CTTCG 2238830 1.2248516 110.490845 3
ATATT 1323680 1.2222284 25.321613 240-247
ATTAT 1319065 1.217967 9.392693 50-59
CGGCT 2868775 1.2171781 20.963306 230-239
CTTCT 1701110 1.2000506 22.668163 30-34
GATCC 2368070 1.1997668 24.550217 70-79
CGCTG 2818695 1.19593 12.500382 190-199
GTCGA 2460155 1.1941838 13.132444 150-159
ACACA 2119215 1.1873034 7.4355855 130-139
GACCC 2843335 1.166055 45.17211 10-14
AGCTC 2300930 1.1657505 12.822412 210-219
TGGAC 2395560 1.1628287 15.188489 80-89
TTCGG 2216730 1.1619345 105.904625 4
TGAGT 1930840 1.1578872 19.926325 90-99
TACCA 1911560 1.1564711 10.615028 45-49
GTAGA 2082275 1.1563728 17.88512 170-179
TTGGG 2298900 1.154504 16.328733 100-109
GAACC 2457820 1.1531669 11.0752 180-189
TCGAG 2361995 1.146536 12.814629 150-159
GGGTG 2940040 1.1450484 14.082842 60-69
GGACT 2353790 1.1425532 13.628474 90-99
GCCCC 3180780 1.1401803 12.77539 80-89
ATTCC 1741460 1.137681 22.253746 180-189
ATTAG 1574005 1.1271213 23.85006 110-119
GTATA 1569805 1.1241138 10.231295 240-247
AAGGC 2493795 1.1210095 27.206882 25-29
ACGCA 2388875 1.1208191 20.327707 140-149
GAAGT 2003140 1.1124258 12.585654 140-149
GAAGC 2472130 1.1112707 13.70102 200-209
AGGCC 2820665 1.1082786 13.209204 2
CCCCT 2373560 1.0970966 10.268256 140-149
GGAGA 2544595 1.0959071 85.65019 7
TAGAC 1890555 1.0958287 18.307663 170-179
GCACC 2671980 1.0957822 11.498512 130-139
CGCAG 2773425 1.0897175 16.118326 140-149
GCAAG 2413415 1.0848773 16.29974 25-29
AGGTC 2222385 1.0787679 17.239769 10-14
CACAT 1780375 1.0771058 13.740426 130-139
ACAAG 1997475 1.0721968 16.409506 80-89
ATCAA 1542740 1.0678018 12.828758 110-119
GCTTC 1947165 1.0652834 28.153412 2
TGGCT 2031790 1.0649953 32.631893 40-44
GCTCT 1942260 1.0625999 18.33742 210-219
TACAG 1819845 1.0548427 9.126802 120-129
CCGAT 2081435 1.0545449 22.597696 160-169
ATGGT 1746445 1.047309 10.13728 35-39
TTGAG 1743210 1.0453691 19.715687 90-99
CTTGA 1667850 1.0439278 21.251343 90-99
CTCCG 2348550 1.0400422 9.768366 150-159
ACCGC 2524020 1.0351034 11.47413 220-229
ATGAG 1855480 1.0304242 19.579885 60-69
ATGGG 2207215 1.026502 18.961666 100-109
CTATA 1370775 1.024529 12.717987 50-59
ACCAA 1827140 1.0236665 17.76742 130-139
GGGAG 2837500 1.0234022 13.903488 5
GAGCC 2594770 1.0195214 17.635715 220-229
CAGCA 2172205 1.0191612 11.651787 45-49
CGTAT 1625440 1.017383 7.6165643 240-247
GTAGC 2091665 1.015315 11.202584 50-59
TAGTT 1310800 1.0135874 15.176657 50-59
CCAAC 2062460 1.0099994 15.040591 130-139
ACACC 2048180 1.0030065 12.65657 40-44
CGAGT 2062785 1.0012965 15.389224 160-169
CGTGA 2057900 0.99892527 20.198286 150-159
TTATA 1068200 0.98632926 13.37682 110-119
GACAG 2191615 0.98517364 14.796475 70-79
AGCCG 2490130 0.9784068 18.569838 220-229
TTTCA 1205810 0.9731888 6.755932 100-109
ATGAT 1354520 0.9699515 9.0145645 120-129
CGAGG 2568690 0.9669759 14.024771 220-229
TGAGG 2076635 0.9657736 11.296984 220-229
CCGTC 2179000 0.96495795 10.568122 140-149
ATGAC 1663975 0.9644953 10.454765 160-169
GGTAT 1607355 0.9638996 9.42895 110-119
GGGAC 2555920 0.9621687 10.504351 90-99
AGCCC 2338145 0.95887595 11.944853 80-89
AAACT 1381890 0.95647025 16.056889 130-139
CCCCA 2231030 0.9549704 17.630074 80-89
ACTCA 1577595 0.9544262 11.463836 190-199
TTTAG 1233200 0.9535824 29.089453 45-49
AATCA 1367735 0.94667274 14.497515 110-119
TATGG 1569875 0.9414235 13.242471 35-39
AACTC 1555180 0.9408655 15.416485 190-199
CGGAC 2391280 0.9395674 11.353331 220-229
AAGGT 1686650 0.93666595 19.763517 15-19
CCCAG 2278735 0.9345118 15.390922 80-89
CCGAG 2374205 0.9328582 17.48893 220-229
GCAGT 1919655 0.93181974 5.9378133 50-59
CATTA 1241435 0.9278592 11.339433 110-119
GCCAA 1973085 0.9257376 13.914436 180-189
TGGTT 1424265 0.92229724 9.877281 50-59
GATAC 1590075 0.92166036 22.681946 35-39
GCCAT 1818165 0.92116106 7.7120447 60-69
CATAC 1517515 0.91807854 13.944009 130-139
TCATA 1221310 0.9128176 8.881028 110-119
CGGAG 2423850 0.9124513 75.1531 6
GACTG 1873770 0.9095467 13.262353 90-99
TCCTT 1287355 0.9081665 11.346671 45-49
CAGAA 1681290 0.90247625 17.043995 140-149
ACAGG 2005935 0.9017069 14.343645 70-79
TTTAT 895980 0.8933627 9.679239 240-247
TACAC 1474375 0.8919794 20.670294 35-39
TAACA 1283040 0.8880514 8.164429 120-129
GAGAT 1595060 0.88580227 11.348084 210-219
ACGCT 1745355 0.8842722 15.215846 190-199
GTTAT 1130610 0.8742538 10.910036 110-119
AACTG 1505770 0.8727945 10.657129 60-69
TCTTC 1234275 0.87072116 48.819767 2
ATAGT 1201180 0.860147 15.628883 120-129
GGTAG 1844000 0.857583 11.894956 120-129
GTCTG 1635595 0.85732335 16.24415 3
AGAAG 1661860 0.85466135 14.800849 140-149
TATTT 855005 0.8525074 10.185982 240-247
AAGTT 1185750 0.84909785 16.206388 140-149
GTCTA 1344380 0.84146404 10.728562 30-34
GATGA 1514720 0.84118617 6.4144683 60-69
AGTCT 1335030 0.83561176 15.477105 8
GATCA 1430740 0.8293046 9.001221 110-119
ATATG 1152910 0.8255815 5.5933957 50-59
GGTTC 1571910 0.8239418 11.188869 160-169
GCCTA 1622615 0.822087 15.97821 190-199
ACAAA 1282110 0.8217942 12.247567 130-139
CCTCC 1772110 0.8190971 11.300831 140-149
TTACC 1253195 0.8187016 9.751239 45-49
CATGG 1683775 0.8173212 8.291798 200-209
CGCCG 2373595 0.8151791 11.5357065 220-229
TGGCA 1674960 0.8130423 5.7620254 100-109
ATCCA 1336610 0.8086332 9.223417 180-189
ACATC 1332075 0.80588955 5.7907715 110-119
CTATC 1224070 0.7996745 7.8851514 110-119
GCTTG 1523725 0.7986849 16.375942 90-99
GGTGC 1955230 0.79480755 11.189806 60-69
TCATC 1214350 0.79332453 9.992758 110-119
ATATA 919595 0.7863317 9.684426 130-139
TGATG 1298575 0.7787301 11.644199 120-129
TTATG 1001695 0.77456915 10.537399 50-59
CCCCC 2055940 0.76920813 14.134724 80-89
TATAC 1026655 0.76733077 8.19172 130-139
CCAGC 1852305 0.75963235 6.123031 80-89
ATTAA 887355 0.75876373 6.09536 110-119
GGTTA 1262855 0.7573096 8.701341 50-59
CTGAC 1476640 0.7481297 9.443753 220-229
GACCG 1894450 0.7443558 9.89024 210-219
CATCC 1404960 0.7429502 6.2927623 110-119
GTTCA 1184265 0.741246 13.424978 160-169
GCGGA 1968355 0.7409816 9.613345 220-229
ATAAA 932210 0.7381809 7.7456555 130-139
TTGGA 1225920 0.7351604 6.442299 60-69
AAATA 922160 0.7302227 12.225737 130-139
TCCGG 1711520 0.7261721 12.342993 70-79
GGGAT 1548095 0.7199674 12.767659 100-109
ATCTT 891625 0.71961546 14.171872 3
TCAAC 1186550 0.71784866 7.4134517 110-119
ATGCC 1405970 0.7123253 8.091404 50-59
TATTG 914225 0.70693225 6.118772 240-247
TGCCA 1389590 0.70402634 7.456576 60-69
GATTA 976365 0.6991604 5.635854 50-59
TTTAC 866165 0.6990671 13.098585 45-49
CCGTA 1365525 0.691834 6.4219427 230-239
CACAA 1234765 0.69178474 8.104817 130-139
GACCA 1470380 0.689877 5.9655857 170-179
AACGC 1469620 0.6895205 13.591042 180-189
AATAC 994980 0.6886718 11.117167 130-139
GAAAT 1038310 0.68854356 7.5921817 110-119
ACGCC 1671285 0.6853959 12.308868 180-189
GCCGC 1993155 0.68452215 12.586585 220-229
AATAA 859550 0.6806443 7.3209696 130-139
GGGCA 1805625 0.6797223 12.980942 150-159
GGATA 1222675 0.67900175 14.905002 35-39
TTTCT 774935 0.67537296 9.607836 2
CAAAC 1195530 0.6698031 12.999493 130-139
CAACG 1421735 0.6670536 13.848041 180-189
CCCGT 1494400 0.6617866 10.335876 140-149
CACGC 1612410 0.6612511 12.410864 190-199
TATAG 922245 0.66040576 5.9954414 120-129
GAAGA 1278975 0.65775126 6.192316 15-19
CAGTC 1296035 0.6566273 5.2147365 210-219
AGGCA 1436960 0.6459415 6.5422406 80-89
TCAAT 860715 0.6433058 8.846694 110-119
ACTTC 977925 0.63887006 30.687826 4
CCGGC 1859285 0.63854617 10.03826 70-79
AGATG 1139490 0.6328056 5.451703 120-129
GCGAC 1591365 0.6252696 6.9335704 70-79
AAGCA 1156055 0.62054265 6.008406 120-129
TACTT 768190 0.61999315 11.60004 1
TCCAC 1171785 0.619646 9.885657 180-189
GACGA 1376375 0.61870736 9.442732 220-229
CACCG 1488375 0.6103843 9.254445 45-49
TAGCT 966810 0.6051383 12.214662 50-59
GGCCC 1755920 0.60304695 10.139599 70-79
GTCCT 1093435 0.5982123 6.82273 45-49
GGTGA 1272350 0.59172755 6.096264 120-129
TGATT 764635 0.5912605 6.152881 50-59
CCCTA 1116445 0.5903819 11.30379 110-119
GGGGA 1625970 0.58643913 9.056063 4
AATGG 1051805 0.58411056 6.5442524 120-129
ACCCG 1420690 0.5826266 8.908509 140-149
CTTTG 860720 0.5817488 7.339653 45-49
CACGA 1233185 0.57858914 8.36516 180-189
AAGAT 868065 0.5756475 5.5289693 15-19
ACGAC 1226185 0.5753049 8.909188 220-229
TAATA 667880 0.57109404 8.020691 130-139
CGGCA 1443895 0.56732655 11.400332 70-79
ACCTA 933440 0.56472003 6.7673373 130-139
CTGCG 1324125 0.5618063 12.601549 25-29
TCAGG 1151360 0.55888164 5.458364 150-159
TTAGC 890180 0.5571746 9.293704 45-49
AAATC 790650 0.54724556 8.226089 110-119
GTTCC 999705 0.5469332 6.116691 140-149
ATTTC 677315 0.5466495 6.0283413 240-247
CGGTT 1042030 0.54619676 11.083625 160-169
ACCCC 1271045 0.54405826 9.563691 140-149
TTCAA 723335 0.5406269 5.1776 200-209
GCTAC 1036810 0.5252928 7.803715 50-59
TATGT 672580 0.52007824 6.3665104 140-149
CCACG 1260710 0.5170186 7.412099 180-189
CTGTT 761120 0.5144306 5.48114 240-247
GGGTA 1074350 0.49964437 5.423248 100-109
CGCGG 1501185 0.49395472 6.8478694 220-229
GGGTT 975905 0.49009794 5.4334016 100-109
TGACG 995755 0.4833494 8.495796 220-229
ACATT 642660 0.4803296 5.566483 110-119
GTAAC 824655 0.47799748 6.5433908 120-129
TAAAT 552275 0.47224197 7.8106656 130-139
CAATC 779265 0.47144607 6.710963 110-119
AACAT 681080 0.47140712 5.557565 110-119
TGGCC 1105755 0.46915522 14.670536 2
TATCC 715410 0.46737126 5.290274 110-119
CCGCG 1344680 0.4618121 7.0931425 220-229
CCGGT 1079470 0.45800284 8.623197 160-169
TGCGC 1065130 0.45191866 9.368002 25-29
ATGCT 716110 0.4482221 6.724261 50-59
CATAA 642560 0.44474554 5.6611567 110-119
TGCTA 699775 0.43799782 5.917949 50-59
GCGCT 1023965 0.43445295 8.64495 25-29
GCCTT 792870 0.43377492 9.246584 1
TGGTC 821315 0.43050542 5.58958 8
ATTCT 532495 0.42976773 7.453917 2
GGTCA 870045 0.42232856 5.3175774 160-169
GCATG 856015 0.41551825 8.011353 60-69
CACTT 619995 0.40503746 7.2145057 3
ATGGC 833425 0.40455282 7.1074066 1
CTAAC 654390 0.3958982 5.255332 110-119
ACGAG 877510 0.39445785 7.2800527 180-189
CCGGG 1197565 0.39405054 5.3800087 80-89
AGCTT 625605 0.39157388 7.272809 1
CATGC 772145 0.391202 6.9240484 60-69
TTTGA 500805 0.38725173 6.0491986 45-49
CCTAA 639885 0.38712278 7.274866 110-119
TTCTT 435360 0.37942588 20.659203 1
GGTAA 674990 0.3748497 6.793694 120-129
GAGGC 966705 0.3639133 5.3471484 35-39
TGGGC 862115 0.3504526 8.639862 1
TGCTG 650705 0.34107745 13.112809 1
TCGGT 643590 0.33734804 5.525059 7
CGAGC 847120 0.3328453 6.248086 180-189
AACCT 542280 0.328073 5.28031 2
CTCTT 455565 0.321379 9.685324 1
CGCTT 555000 0.30363753 5.936654 1
AACTT 404845 0.30258468 10.675561 3
CGGGA 801705 0.30179954 5.124434 80-89
GACTT 478175 0.29929563 6.6326594 1
GCGAG 790075 0.29742146 5.1739182 240-247
ATTGT 369835 0.28597808 5.077482 240-247
TTCCT 397905 0.28070268 6.5983214 4
GAGGT 587925 0.27342433 6.243543 7
TGTAA 380795 0.27268156 7.8557696 25-29
CCCTT 468005 0.26724306 5.8919697 3
GTCTT 394455 0.26660675 12.270701 1
TAACT 356480 0.26643622 7.132663 2
TTGGC 483790 0.2535863 7.2637444 1
AATCT 338575 0.25305387 7.423472 2
CGGGG 791635 0.24956536 9.066153 3
TCCTA 368595 0.24079998 5.2669353 9
CCTCG 541060 0.23960537 5.1913295 5
TGCTT 332240 0.22455648 11.408297 1
TCGCA 362205 0.1835087 7.033274 7
TAGGC 321145 0.155887 6.301573 1
TTGCT 188205 0.12720518 5.4743743 4
Produced by FastQC (version 0.10.1)