FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_7_SL248353.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_7_SL248353.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5929637
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 6745310 8.576239 59.710686 110-119
ATACA 6209830 7.4472713 36.45273 110-119
CACAG 9074045 7.3942056 41.045864 110-119
TCCCT 10413245 6.8685064 101.93279 30-34
CAGTA 7466790 6.450628 38.24322 110-119
AGTAA 5880530 6.3225274 46.450184 110-119
GGAGA 9342795 6.119008 94.00781 35-39
GTAAT 6380730 5.844075 38.61972 110-119
GGTGA 10471945 5.8425536 101.40204 45-49
CCCTG 10221820 5.7014303 66.71304 30-34
AATAC 4520905 5.4217916 38.388634 110-119
AGACG 7344110 5.365208 101.130325 40-44
GTAGT 8092970 5.3395414 32.096134 80-89
ACAGT 5990935 5.175623 34.828205 110-119
GTGAC 8068080 5.020983 84.56133 45-49
TAGTA 5239880 4.7991776 41.36333 80-89
CCCCA 6525700 4.766007 100.863846 60-69
GAGAC 6503465 4.7510786 140.43721 40-44
CTCAG 6711790 4.65908 34.263157 140-149
ACTCC 5846545 4.526935 114.525734 30-34
GGCCC 9385915 4.42702 57.79404 60-69
ACCAG 5384540 4.387723 88.34171 45-49
GCACA 5332685 4.345468 33.688705 110-119
GTGTC 8145840 4.3184276 23.196232 120-129
AGTAG 5530200 4.2831736 33.369843 80-89
TGACC 6154485 4.2722187 144.99759 45-49
CGTGT 8046340 4.2656794 22.655178 120-129
CACTC 5359155 4.1495533 94.10849 25-29
CTCCC 6516165 4.054066 65.92309 30-34
CCCAG 6082020 3.9822838 86.41086 60-69
CCCTT 5816570 3.8365705 41.34799 20-24
ATGCA 4436955 3.8331256 91.75374 25-29
GACCA 4683145 3.8161743 102.068886 45-49
TGAGG 6738375 3.7595038 68.64399 35-39
TACAC 3831845 3.6924887 26.52957 110-119
AGGAG 5620285 3.6809723 94.17382 35-39
ATGGT 5561665 3.6694489 25.016527 190-199
GACGG 6971365 3.66873 107.96199 40-44
ACGGT 5841080 3.635061 96.076454 40-44
CCACT 4678860 3.6228058 26.785349 240-247
TGTCC 6091520 3.6021242 20.025364 120-129
AGATA 3347640 3.5992584 18.791782 150-159
CTGAG 5754120 3.5809438 103.51257 35-39
CAGGG 6685625 3.5183573 33.4202 50-59
TGGTG 7392800 3.5136259 18.869354 25-29
GAGGA 5303155 3.47327 123.936295 35-39
GCCCC 6555575 3.4489663 72.63108 60-69
CTTGG 6477665 3.4340632 23.655333 20-24
TGCAC 4885400 3.3912663 110.561104 25-29
AGGCT 5449255 3.391218 29.051506 30-34
CCGTG 6770780 3.3857198 22.803253 120-129
CAGAT 3869060 3.342516 17.39238 150-159
GGGTT 6898375 3.2786374 39.493347 50-59
TCTTG 5220795 3.273008 17.546528 170-179
GGATG 5865705 3.27262 67.27348 20-24
CTCTC 4917020 3.2432334 13.367208 100-109
CCTGA 4664300 3.2377868 80.83962 30-34
TCAGG 5160420 3.2114682 20.494007 140-149
CTTCA 3893435 3.1960635 16.084976 200-209
CCAGG 5332485 3.1301906 37.90551 50-59
GCACT 4494605 3.119991 85.5741 25-29
TCGCA 4484410 3.1129136 23.276648 100-109
TCTCA 3756345 3.0835283 21.090345 130-139
TGTCT 4905065 3.0750713 19.121206 180-189
CCTGG 6132465 3.0665312 27.728981 50-59
ACAGA 3007960 3.0504854 13.971186 140-149
CGCAC 4632120 3.0329428 23.821142 100-109
GCCCT 5319815 2.9672363 22.190437 20-24
GGTTG 6176540 2.9355657 70.55202 15-19
CGGAT 4674610 2.9091356 76.228485 20-24
GACTC 4114970 2.856462 18.939903 190-199
GATGG 5110755 2.851415 22.86397 15-19
TGGCC 5669820 2.8351865 42.118267 60-69
TTCCC 4293520 2.831977 46.575573 50-59
ACCAC 3113605 2.830071 18.175524 230-239
GATGC 4493645 2.7965162 91.802124 25-29
TCTCT 3995800 2.7941995 14.543699 180-189
GGTTC 5262055 2.7896206 44.53968 50-59
AGGGT 4951115 2.7623482 47.079693 50-59
CGGTG 6134930 2.7502916 67.94244 40-44
GCCGT 5463800 2.7321665 21.561695 120-129
GTTGG 5702305 2.7101731 50.889477 15-19
CGGCC 5725380 2.700469 12.442331 120-129
TCCAT 3282920 2.6949008 20.791779 70-79
TTCTT 3624670 2.6871972 19.969608 170-179
TCAAA 2226440 2.670106 54.59338 70-79
TCACA 2746215 2.6463408 11.328409 140-149
AGATG 3372010 2.6116424 16.445192 180-189
TGGAG 4649880 2.5942814 19.934135 25-29
GGCTG 5761825 2.5830286 15.113989 30-34
AGTCA 2965625 2.5620303 28.723167 70-79
CCAGT 3666775 2.5453413 33.563972 60-69
AGGGA 3867830 2.5332122 8.88728 150-159
GTTCC 4282560 2.5324242 50.34932 50-59
TCCTC 3757675 2.478537 16.539476 130-139
ACTCT 2977305 2.4440255 13.464693 200-209
TGTTC 3897195 2.44322 12.468967 140-149
GCGGA 4562620 2.4011111 83.60082 20-24
GAGAT 3084525 2.3889835 15.999998 180-189
ACAGC 2914270 2.3747637 10.849224 120-129
TGTAG 3588710 2.367742 7.79519 210-219
AATAA 1581510 2.3604548 10.139812 110-119
GGCGG 6208115 2.353468 40.419033 20-24
AAAAA 1339200 2.346378 8.628244 130-139
CTGGA 3737190 2.325754 14.135032 180-189
GTTCT 3695615 2.316846 16.834455 160-169
TACCA 2399630 2.3123603 15.419046 230-239
CACGG 3914865 2.2980418 9.53486 120-129
CAGCT 3302140 2.2922251 11.944136 150-159
TTGGG 4765455 2.26491 76.63742 15-19
CAGAG 3055100 2.2318902 9.094166 140-149
CATTT 2558870 2.2269378 17.280794 150-159
TCATT 2542755 2.2129133 16.090096 150-159
TTCAT 2534170 2.205442 15.390921 140-149
CTGGC 4399045 2.199737 32.539406 60-69
GTGAA 2823130 2.1865313 17.300835 190-199
TCAGC 3127790 2.171198 13.932281 140-149
TTCAC 2630800 2.159585 17.632006 200-209
TTGTC 3444405 2.159358 12.755819 170-179
CTACT 2612495 2.1445587 7.4344263 230-239
GATAC 2480680 2.143082 14.055432 150-159
ACTAC 2220695 2.1399329 12.015719 230-239
ATGTA 2333855 2.1375651 9.308694 70-79
ATTTG 2682230 2.0927277 15.192906 150-159
CCAGA 2563105 2.0886083 31.614502 60-69
CACTA 2163730 2.0850396 14.445345 240-247
CTCGC 3737705 2.084782 16.018864 100-109
TCTGC 3515805 2.0790157 11.623951 210-219
CTCTG 3489915 2.0637062 14.041821 180-189
GAACT 2384120 2.0596628 10.459513 160-169
GAATC 2377125 2.0536199 14.036783 190-199
GCTGT 3863980 2.0484467 9.828581 140-149
TCTCG 3442665 2.0357656 15.691883 100-109
ACACA 1795855 2.0314744 13.324605 110-119
TTGGA 3065735 2.0226963 15.025099 170-179
ATCTC 2463915 2.0225914 7.917621 100-109
GTCCT 3419225 2.0219045 14.060119 130-139
TGGGG 5014935 2.0155392 50.534077 15-19
TGAAT 2198375 2.0134795 13.583002 190-199
GTCTC 3404905 2.0134368 11.456062 180-189
AGCTC 2895385 2.0098705 11.986283 140-149
TACAG 2320475 2.0046794 13.227643 160-169
CAGGC 3386435 1.9878513 14.984778 140-149
GATAT 2167080 1.9848167 8.026584 180-189
GTTCA 2694155 1.9827219 14.4244995 140-149
AATCG 2290065 1.9784079 13.004271 190-199
TTTGC 3148975 1.9741477 11.677319 150-159
ACGGC 3342850 1.9622667 14.17979 120-129
ACACG 2404265 1.9591737 13.391475 120-129
TGGAA 2519790 1.9515927 8.622342 170-179
ATATA 1534270 1.950728 10.123835 110-119
TAGTC 2647435 1.948339 23.73582 70-79
TTGCA 2636990 1.9406521 13.711749 150-159
AATAT 1502535 1.9103789 12.70378 110-119
GACGT 3063855 1.9067192 10.013224 60-69
CCTTC 2884880 1.9028473 13.255109 200-209
AACCA 1682135 1.9028338 14.319736 70-79
ATATG 2069480 1.8954254 8.24633 180-189
CCTTG 3200785 1.8927335 26.887787 20-24
GAGTC 3027515 1.884104 11.700942 210-219
CAGCC 2867405 1.8774717 9.960373 120-129
TACTA 1831920 1.8715229 9.890199 240-247
CATAG 2154190 1.8610243 8.136189 210-219
TCGGC 3711960 1.856161 7.490105 130-139
GTCAA 2137990 1.847029 36.740856 70-79
GTTGT 3192895 1.7945347 6.782175 210-219
CACCA 1967300 1.7881519 12.632418 230-239
GGGGC 4711400 1.7860701 45.992115 15-19
AGTAT 1947550 1.7837503 11.462466 220-229
CTGTT 2845125 1.7836587 12.179279 140-149
CGTAG 2846510 1.7714598 9.322007 210-219
AGAAC 1728205 1.7526375 11.024246 160-169
CTCCA 2246920 1.7397733 7.74041 150-159
GTCCC 3118600 1.7394631 23.48072 50-59
GGGCG 4586670 1.7387855 56.32757 20-24
ATTGT 2195550 1.7130104 8.465417 50-59
GCAGA 2340055 1.7095172 13.843925 150-159
GTCAC 2456785 1.7054105 8.355219 140-149
GGTCA 2733135 1.7009034 7.446047 130-139
TTGGT 3010095 1.6917938 28.494526 25-29
TGACT 2294335 1.6884805 11.464832 190-199
TGCAG 2705625 1.6837833 12.88686 150-159
TCCCA 2167670 1.6784105 10.937068 240-247
TCAGA 1939050 1.6751629 10.241168 130-139
CAAAA 1189750 1.6749506 21.983885 70-79
AGTCT 2267490 1.6687243 12.317828 210-219
ATAGT 1818270 1.6653435 7.143147 220-229
CTGTG 3081420 1.6335812 5.3299184 210-219
CAGAC 2000950 1.6305226 19.08797 60-69
GAGAA 1788135 1.6257507 10.312502 160-169
AACTG 1878875 1.623177 10.278576 160-169
TGGTT 2884100 1.6209794 17.50672 10-14
TGTGC 3027720 1.6051127 7.896786 220-229
TTCAG 2180790 1.6049188 9.1276245 150-159
AATAG 1483930 1.5954665 8.940473 240-247
GCTCC 2849070 1.5891272 6.241901 150-159
TGCTC 2677760 1.583451 5.4209085 150-159
ATAGA 1467430 1.5777264 9.837591 240-247
ACTGA 1823275 1.5751438 8.416846 180-189
GTCTG 2969845 1.5744308 8.532098 210-219
CTGGT 2952675 1.5653284 6.769986 160-169
TCTGG 2950310 1.5640746 11.417094 180-189
ACCAT 1621915 1.5629293 13.016489 45-49
TTGTA 2000395 1.5607467 8.618392 210-219
TAGTT 1990210 1.5528003 9.384502 220-229
GGCCG 3671590 1.552551 12.749853 120-129
GGACG 2942650 1.5485904 6.9731793 170-179
GCTCA 2229905 1.5479187 10.816988 140-149
GTGCT 2916715 1.5462645 7.537981 220-229
GCTGA 2471260 1.5379316 24.611937 30-34
GAACC 1881970 1.533569 11.869731 70-79
CCCAC 2082865 1.5212083 10.298136 240-247
CATGT 2058760 1.5151128 9.328097 70-79
CCACC 2062640 1.5064371 9.096344 230-239
CAAAG 1441835 1.4622191 26.164322 70-79
CCTCG 2618375 1.4604522 9.26952 130-139
GTGGA 2611295 1.4569052 25.286877 25-29
ACCGT 2095585 1.4546787 22.538908 45-49
AGATC 1680380 1.4516956 6.986256 130-139
GCTCT 2434300 1.4394847 9.461257 130-139
CGACT 2062360 1.4316152 8.353811 190-199
TCGAC 2022970 1.404272 9.010904 190-199
TGATA 1527705 1.3992167 7.4200463 180-189
CACTG 2014565 1.3984375 6.958408 230-239
CCTCA 1798715 1.3927312 7.3482885 130-139
CTCTT 1990555 1.3919635 7.9133883 200-209
TGGAC 2224485 1.3843569 9.96575 170-179
CCATG 1987685 1.3797784 9.464672 70-79
GTCCA 1981750 1.3756585 19.439539 70-79
GTAGG 2457320 1.3709989 5.233285 160-169
GAGGG 2870825 1.3544453 7.85092 200-209
GGGAG 2868095 1.3531573 6.404677 150-159
TATAC 1321415 1.3499819 6.4961123 110-119
GCGTA 2163740 1.3465537 9.829655 210-219
GGAGG 2841135 1.3404377 19.080835 30-34
ACTTC 1631450 1.3392332 6.5762053 220-229
AATTG 1461770 1.3388271 8.528687 170-179
CCATC 1729090 1.3388212 10.6928625 240-247
CTGCT 2259035 1.3358445 5.4497533 90-99
GTGGT 2795310 1.3285458 12.916209 50-59
TATGG 1998050 1.3182639 6.0734625 180-189
CTCGA 1895640 1.3158842 8.038208 190-199
CTTGA 1785635 1.3141105 8.115312 200-209
TGGTC 2476620 1.3129529 14.52534 50-59
TCCAC 1678595 1.2997235 9.422477 240-247
CTGAT 1765120 1.2990129 7.0910535 180-189
ACGGA 1774930 1.2966675 8.835116 200-209
TTGGC 2422845 1.2844448 9.2901535 60-69
GTGTT 2272000 1.2769548 7.216512 160-169
TGCCC 2266055 1.2639387 8.271234 60-69
AGCCG 2152080 1.2632798 8.863211 120-129
ATCGG 2027180 1.2615687 7.7297516 190-199
ACGTG 2021330 1.257928 8.977459 170-179
GAATT 1363415 1.2487444 9.506794 170-179
ACTAT 1209090 1.2352285 6.277724 230-239
ATAAA 825255 1.2317199 5.4372354 7
GGAGT 2196630 1.2255536 6.5865264 210-219
GATCT 1658790 1.220761 5.5310473 130-139
GGCTC 2438205 1.2192214 7.9141326 130-139
ACTCG 1755710 1.2187498 8.211609 190-199
AAGTA 1126755 1.2114451 14.984076 70-79
ATAAC 995785 1.1942165 6.908026 80-89
TTGAG 1809385 1.1937875 7.82916 200-209
GGAAT 1536035 1.1896685 7.997703 170-179
GCATA 1373100 1.1862335 7.67897 210-219
CCATA 1230505 1.185754 11.872214 70-79
ATACC 1229200 1.1844965 8.615482 70-79
ACTGG 1899680 1.182222 6.825526 160-169
ATGGG 2088535 1.1652447 35.95213 15-19
AAAAG 917310 1.1577617 15.125211 70-79
ACTGT 1570940 1.1561091 5.372802 230-239
TTCCA 1398375 1.1479055 7.1806884 230-239
TCTGT 1825080 1.1441747 6.4205794 210-219
TATCA 1117530 1.1416892 7.3576703 70-79
AGCTT 1549225 1.1401283 5.966996 150-159
AGAGC 1558910 1.1388551 6.731157 140-149
GAAGA 1249770 1.1362758 19.749123 35-39
AAAGT 1051075 1.1300768 19.00555 70-79
AGAGA 1237170 1.12482 14.478408 35-39
CAATA 937740 1.1246048 6.5646424 100-109
AGTTC 1525250 1.1224842 5.407387 160-169
TCACG 1591385 1.1046815 8.8307295 200-209
ATCCA 1144725 1.1030936 15.116407 240-247
TCTAC 1334480 1.0954549 6.977237 180-189
CTGAA 1264975 1.0928234 15.807949 35-39
ATATC 1067765 1.0908483 7.8481565 70-79
GTCTA 1474730 1.0853049 7.181565 180-189
AACTT 1061410 1.0843558 6.0438066 200-209
AGGTT 1641125 1.0827737 22.511585 10-14
CTGCC 1906840 1.0635791 5.831698 200-209
CTCGG 2118435 1.0593208 7.884123 130-139
CGGCT 2116055 1.0581306 7.4607744 130-139
TACTG 1434755 1.0558858 6.137049 180-189
GTCCG 2090465 1.0453343 5.0308433 130-139
GCTTC 1764915 1.0436546 5.2924566 150-159
TATGT 1337640 1.0436525 7.171958 220-229
GAGCT 1673455 1.0414361 6.049275 140-149
CATGG 1658725 1.0322692 5.318205 190-199
TCCCC 1649120 1.0260086 5.36023 80-89
CTTCT 1464675 1.0242239 5.448034 210-219
CGTGG 2283735 1.0237993 8.842782 50-59
TAGAT 1116555 1.0226465 7.4977374 240-247
CTGCG 2044500 1.0223496 6.493274 210-219
GCGGT 2273285 1.0191146 5.6642213 130-139
ACGTC 1455605 1.0104278 13.349168 70-79
CTACC 1288605 0.99775714 7.886226 230-239
AAGAG 1089210 0.9902965 14.740753 35-39
TGCGT 1850485 0.9810144 7.7858872 210-219
TATAA 767935 0.9763812 6.291536 240-247
GGTGG 2421695 0.97329694 25.197235 25-29
GGGGT 2419340 0.9723505 7.283775 10-14
ACAAT 803980 0.96419024 7.564688 100-109
CCATT 1173310 0.96315277 6.3662767 50-59
CGAAC 1178755 0.96053714 12.479916 70-79
TAGGC 1537185 0.9566316 5.825771 160-169
GAGGC 1808925 0.9519596 16.098185 30-34
CCCAA 1041475 0.94663525 9.418705 240-247
AGCTG 1520415 0.9461952 5.0149603 30-34
ACCCA 1036570 0.9421769 12.211875 240-247
GAGTT 1423255 0.93902844 5.163431 160-169
TGAAG 1211210 0.9380895 10.534125 35-39
CGGAG 1755510 0.9238496 6.182528 210-219
CAGCG 1555070 0.91283244 5.83718 160-169
TGCAT 1238775 0.9116574 5.8993297 210-219
TGTAA 991905 0.9084804 6.2815924 230-239
ACTAA 754550 0.90491027 11.690393 240-247
TTCTG 1439695 0.90257007 5.3622627 210-219
GGGCC 2132980 0.9019417 19.044828 15-19
ATCAA 749570 0.8989379 13.984253 70-79
CGTCC 1600495 0.8927089 10.924821 70-79
TCGAA 1023855 0.8845176 16.39617 70-79
GACCG 1504585 0.88319755 20.667017 45-49
CATCA 910650 0.87753147 5.3544803 230-239
CCAAT 894215 0.8616942 10.222747 240-247
TGGGC 1919465 0.8604969 20.687643 15-19
CCCCC 1457620 0.85539293 5.0791035 80-89
GGTCC 1704965 0.85256565 14.646726 50-59
CGGTC 1682495 0.8413295 5.386577 130-139
TGAAC 972705 0.84032863 6.104289 190-199
CCAGC 1268305 0.8304396 5.477201 80-89
GTATG 1254385 0.82761216 5.0855227 220-229
CTTCC 1214830 0.80129373 5.032294 230-239
AGCGT 1283780 0.79893076 5.9747076 160-169
AAAGA 630290 0.79550606 5.751534 8
TGAGA 990945 0.7674929 5.4506135 240-247
CATAC 795730 0.7667909 10.306618 70-79
AACCG 939505 0.7655784 5.341647 190-199
TAACC 792485 0.76366395 5.545227 80-89
CCTTT 1061675 0.7424124 5.604321 50-59
AATCC 766605 0.7387251 9.090523 240-247
AAAGG 807315 0.734001 8.719593 10-14
ATGAT 798975 0.7317768 6.6213894 240-247
AGCCA 884895 0.72107816 5.534929 80-89
CTAAT 705330 0.72057813 8.940607 240-247
GTCGA 1146515 0.7135071 11.375906 70-79
GTAGC 1142230 0.71084046 5.3905263 80-89
AAAGC 700285 0.7101852 10.304682 70-79
CATTG 944755 0.69527787 5.82443 50-59
GGAAG 1056315 0.69182724 6.925872 30-34
AAGGT 883675 0.68441164 9.538653 10-14
CGATG 1097015 0.68270195 15.858815 15-19
TAGGT 1034510 0.6825441 5.348702 10-14
AGGAT 875490 0.67807233 6.785445 10-14
GAGGT 1180170 0.6584457 6.604588 10-14
CCGTA 941935 0.6538569 5.5084095 80-89
TATGA 709450 0.6497814 5.5603843 240-247
AGAAG 710965 0.64640075 5.3084836 9
CAATC 660625 0.6365994 8.794636 240-247
ATAGG 798500 0.61844313 5.224783 10-14
CATCC 782485 0.60587215 7.469919 240-247
TAAAA 395255 0.5899309 5.6540775 8
GAAAA 460200 0.58083093 5.865411 8
AAGAA 455350 0.57470953 5.3865056 8
AATGG 741215 0.5740755 6.4731975 10-14
GGGTC 1262315 0.56589633 5.3864512 70-79
AAATG 517670 0.5565796 5.086326 9
AAGCA 542615 0.55028623 7.018895 70-79
GAAGG 826950 0.541606 5.424247 10-14
TTGCC 866735 0.51253 5.779027 60-69
AAGGG 772260 0.505787 6.0562067 10-14
ACGAT 585375 0.5057108 7.09514 10-14
TGATC 679560 0.5001117 5.1612153 240-247
GGTCG 1069225 0.47933403 5.473917 70-79
ATAAG 445275 0.4787432 5.53387 9
GATCC 688385 0.47785175 5.1811814 240-247
GAAGC 619560 0.45261693 5.513847 30-34
TACCG 647415 0.44941187 5.4789424 70-79
CGGTT 823520 0.43658012 7.8586006 10-14
AAGCT 500855 0.43269318 9.2378435 30-34
TAAGG 557615 0.4318762 5.755144 10-14
TAAAG 370950 0.3988317 5.532542 9
Produced by FastQC (version 0.10.1)