FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_11_SL248340.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_11_SL248340.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11974012
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 56942 0.47554654196104035 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 43032 0.3593782935911539 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 42402 0.3541168991646242 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 40958 0.34205744908222907 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39692 0.3314845517108217 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37935 0.31681110725461104 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 37798 0.31566696275233397 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37512 0.31327845671108395 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36739 0.30682280926392924 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 36371 0.3037494868052579 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34598 0.28894241963345285 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33289 0.27801041121388553 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31154 0.260180130101757 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30989 0.25880214584718975 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30864 0.25775821838160845 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30829 0.2574659186912457 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30690 0.2563050713495193 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29792 0.24880549643678326 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29146 0.24341047929465914 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29065 0.24273401429696245 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27931 0.23326350432920895 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27562 0.23018183045081297 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27194 0.22710850799214166 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27156 0.22679115404260494 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26739 0.22330861201742572 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26311 0.21973420437527538 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25513 0.21306977143500438 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25395 0.21208430390749566 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25293 0.21123245909558133 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25205 0.21049753415981212 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24957 0.20842638206809883 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24364 0.20347399017138115 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 23868 0.1993316859879546 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23565 0.19680120581138552 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23086 0.192800875763278 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22773 0.19018688138946244 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 22764 0.1901117186119406 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22131 0.18482526992623693 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21782 0.18191062444233394 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20981 0.175221137242889 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20836 0.1740101813828147 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20773 0.17348404194016176 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20614 0.17215616620394233 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20007 0.16708685443107957 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 19729 0.1647651597476268 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19614 0.16380474647929197 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19599 0.16367947518342224 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19541 0.1631950928393925 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 19535 0.1631449843210446 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18650 0.15575397786472905 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18219 0.15215451596340476 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18152 0.15159497084185317 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17855 0.149114599183632 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17678 0.1476363978923689 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16743 0.13982782044982084 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16479 0.13762304564251315 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16165 0.13500069984897292 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16030 0.13387325818614512 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15926 0.1330047105347815 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15752 0.13155156350269234 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15375 0.12840307826649913 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15011 0.1253631614867264 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14443 0.12061955508312501 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14439 0.12058614940422642 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14404 0.12029384971386367 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13149 0.10981281795942746 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12946 0.10811747975532345 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 12775 0.10668938698240824 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12175 0.10167853514761803 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 12122 0.10123590990221155 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 24125040 7.620997 65.460304 35-39
TATTA 12032465 7.309694 126.145744 240-247
TGGAT 18353065 7.192619 77.86396 35-39
TCACC 18726205 6.4030757 74.120056 160-169
TCTCC 16534605 6.0761895 85.28736 20-24
CTGTG 17370880 5.8974485 85.30605 25-29
TCCCT 15843960 5.822389 72.71211 15-19
AGTGG 18823915 5.7155237 39.47635 100-109
CTGAG 17742265 5.604706 48.816418 15-19
TGTAT 10951130 5.5394897 59.550266 240-247
TTACT 10167120 5.3506474 112.52399 240-247
ATTAC 10243275 5.015897 104.586 240-247
TGGGT 14958310 4.881208 45.10007 100-109
CCTGA 14657695 4.817336 50.249 15-19
CTCTG 13324740 4.7065063 72.75324 35-39
GACAC 15058260 4.604871 45.918514 230-239
ACACG 15025565 4.5948725 48.530865 230-239
TTCAC 10800120 4.5814424 73.30011 40-44
CTCCA 13269615 4.537297 40.160934 80-89
TGAGA 12013135 4.380626 91.88909 15-19
TACTG 10604650 4.323868 79.35302 240-247
TCTGG 12656350 4.2968564 134.42442 35-39
AAGGG 15062765 4.2555156 38.83542 90-99
ATTCA 8619110 4.2205806 108.822426 40-44
CCCTG 14676990 4.1787086 84.460945 10-14
TGGAG 13680455 4.15381 38.444435 90-99
AGCCT 12530690 4.1182837 50.002666 30-34
GAAGG 14557160 4.112673 30.66222 90-99
CAGCC 15508030 4.1083093 54.167152 30-34
CCAGG 15973155 4.067238 52.372234 80-89
ACCAT 10250665 4.0460105 67.13577 160-169
TCAGT 9904475 4.038383 99.5786 7
GAGAC 13536025 3.978654 88.80845 8
GTATT 7824900 3.958126 68.94966 240-247
CACCA 12436055 3.956606 57.113808 160-169
ACTAC 10009750 3.9509196 35.87335 130-139
TCCAG 11889070 3.9074116 39.659996 80-89
TACTA 7898375 3.8676531 28.06803 130-139
GGGTC 14667825 3.85812 123.702545 8
ACTGT 9312790 3.7971334 68.75418 240-247
CACCT 11076710 3.787474 44.5305 20-24
CTGGG 14285580 3.7575772 131.21399 3
TGAAG 10197400 3.718513 38.718304 150-159
GACTC 11217075 3.6865566 50.515514 20-24
GATTC 8909620 3.6327477 57.37234 40-44
GGACA 12342795 3.627927 28.428469 220-229
AGACT 9537790 3.6184716 67.24436 15-19
CAAGA 10155865 3.585054 43.498398 180-189
GCTGG 13467095 3.5422885 32.55979 90-99
ACTGG 11192680 3.5357194 44.37141 60-69
CCTGT 9881345 3.4902453 60.44515 25-29
GAGTG 11257145 3.4180183 39.303272 90-99
ACTCT 7953185 3.373764 66.42336 20-24
TCCTG 9426545 3.329603 63.931442 20-24
TATCT 6301370 3.3162203 47.938248 200-209
AGCTG 10329660 3.2630951 36.894493 60-69
AGACA 9199275 3.2473745 37.893856 170-179
CTCCT 8832720 3.2458763 92.66702 20-24
GGATT 8259430 3.2368946 54.921955 40-44
CTGCA 9802050 3.2215006 46.37515 25-29
GGTCC 11578325 3.168497 140.05437 9
GTCTC 8935420 3.1561303 34.130573 30-34
CATCT 7379015 3.1301994 32.05707 170-179
CCATC 9115890 3.1170082 35.95102 45-49
CCTCA 8951435 3.0607758 84.049255 5
GTCCC 10661695 3.0355084 73.15575 10-14
GGAGT 9773865 2.9676485 36.728695 90-99
AGGGA 10292665 2.9078724 39.785343 80-89
AAGAA 7132255 2.906309 37.211777 180-189
GGCTG 11024455 2.899794 33.673676 90-99
AGTAG 7876060 2.8720288 28.890682 50-59
GTGTA 7270400 2.8492908 36.95539 230-239
GAGCT 9001025 2.843385 29.49383 60-69
TGTGC 8270255 2.8077683 56.39898 25-29
ATCTC 6539010 2.773867 47.15436 170-179
ATCTG 6783520 2.7658663 36.02983 200-209
GTGAA 7584260 2.765623 39.725197 10-14
GGAAG 9716465 2.745085 29.975811 80-89
CAGGG 11201650 2.741532 35.916668 80-89
GACAA 7702950 2.7191668 24.039192 180-189
ACCTT 6295820 2.670705 72.05779 40-44
CTATG 6528085 2.661717 27.786505 50-59
GAACA 7456170 2.6320527 33.969837 210-219
GGGAA 9276015 2.6206496 33.570198 80-89
GAGTC 8205370 2.592041 28.564981 150-159
CCCTC 8696140 2.5759013 27.472034 20-24
CACGG 10113920 2.5753033 43.910767 230-239
ATGAA 5850490 2.5621574 26.719057 210-219
ATCAG 6669065 2.5301273 27.648228 45-49
CAGTG 8007885 2.5296564 77.11576 8
TGCAA 6643145 2.520294 34.73734 200-209
AGGGC 10282930 2.516681 32.711536 150-159
AGAAC 7110315 2.5099647 29.868444 190-199
CTACA 6299050 2.48628 20.173365 190-199
CGCCA 9374580 2.4834664 38.74564 70-79
TATAT 4059830 2.466337 24.015131 240-247
ACGGC 9618285 2.4491 48.618763 230-239
CCAAG 7999935 2.4464087 38.376205 180-189
AGGCT 7724010 2.4399815 20.91442 25-29
GTGGA 8025300 2.4367297 27.039295 100-109
TCTGT 5481240 2.4018924 25.081778 210-219
CTGTC 6793595 2.399604 49.31044 30-34
CACTG 7279300 2.392384 19.927797 30-34
GCAGC 9311870 2.3710778 33.348507 30-34
CTACT 5571920 2.3636248 23.258953 50-59
CTCTC 6309070 2.3184772 95.48285 20-24
CCGCC 10088360 2.3152127 33.224777 70-79
TATGC 5674605 2.3137248 21.485725 50-59
ACAGC 7560280 2.3119612 26.129417 210-219
GTATC 5658530 2.3071706 22.377504 200-209
TGAAC 6068785 2.3023913 36.97271 210-219
GGGCT 8736075 2.2978747 41.979225 90-99
CTTCA 5405895 2.2931962 49.558086 45-49
TGGTG 6984220 2.2790964 34.645725 35-39
GTGGT 6982515 2.27854 31.126648 120-129
TCCAA 5747425 2.2685497 42.430473 180-189
GGCCG 10631835 2.2541535 25.64429 230-239
CAAAT 4912605 2.238324 34.266968 200-209
CAGGC 8785255 2.236986 33.20155 70-79
GCCTC 7832775 2.2300816 74.284874 4
TATCA 4507510 2.2072244 17.04197 170-179
ACAAC 6001220 2.2040217 19.601437 180-189
CAGTA 5773235 2.190265 21.691084 45-49
GCTCC 7672030 2.1843162 33.207203 80-89
TACAT 4451915 2.1800008 18.993975 190-199
GCCTG 7924165 2.168508 30.794188 210-219
AATGA 4921505 2.1553185 25.126556 210-219
AAATG 4904280 2.1477752 32.617622 200-209
CCTTC 5823430 2.1400127 54.47859 45-49
GGGGG 10851970 2.1256347 93.80122 5
GTAGT 5387010 2.1111848 24.716936 120-129
GTGGG 8312965 2.1016884 29.537333 100-109
GCTAT 5154560 2.1016853 34.109295 50-59
GCAGA 7075795 2.0797937 23.83784 140-149
TCTAT 3947005 2.0771892 20.650557 110-119
CCTCT 5592700 2.0552232 47.601986 35-39
TGCAG 6492075 2.0508187 42.211258 30-34
CTCAC 5990660 2.0483942 55.950626 20-24
GCTGT 6009485 2.0402324 19.036861 230-239
TGCAC 6196640 2.0365617 27.288605 25-29
AACTA 4456885 2.030685 37.404404 130-139
AGAGT 5543850 2.0215816 30.992224 150-159
AACAG 5650920 1.9947934 32.582596 210-219
AGCAC 6514250 1.9920814 20.5361 130-139
ATGGA 5453360 1.988584 12.365919 100-109
TGTGA 5055575 1.9812948 19.193693 210-219
CAGAG 6729855 1.9781114 31.739225 170-179
TCTCT 4319245 1.969156 33.338547 35-39
TGAGC 6231080 1.9683714 18.174957 200-209
TGGTA 5014695 1.9652735 31.247967 120-129
GGCTC 7143625 1.9549075 20.804565 40-44
ATATC 3987550 1.9526118 13.716921 160-169
CCGTG 7091975 1.940773 20.75165 230-239
GGTCT 5715300 1.940356 36.973957 15-19
AAAAA 3935580 1.9260049 13.670862 90-99
TTCAG 4716945 1.9232551 46.558464 45-49
GCACA 6249380 1.9110832 18.619375 130-139
CATAT 3902585 1.9110065 14.4776745 160-169
GGATC 6049030 1.9108626 18.249641 70-79
CAGAC 6240650 1.9084135 25.869026 140-149
GGTGG 7545045 1.9075426 18.427814 40-44
CTGTA 4667730 1.9031886 24.021942 190-199
TCCGC 6684260 1.9030862 41.029263 70-79
TCCAT 4469875 1.8961339 27.388308 45-49
GTCAC 5742385 1.8872682 38.621693 160-169
ACCAG 6135800 1.8763497 14.02612 190-199
ACCCT 5483375 1.8749375 44.28937 10-14
CCTGC 6575475 1.8721138 50.779102 20-24
ACTAT 3819940 1.870537 17.154839 130-139
TGGGA 6146750 1.8663437 17.17079 100-109
GATGG 6139450 1.8641273 21.335922 100-109
TGGGG 7346315 1.8572994 121.98198 4
CTATT 3521420 1.8532168 19.79849 110-119
GCTGA 5841810 1.8454028 27.67751 200-209
GCCAG 7244005 1.844538 34.558384 70-79
TGTCT 4203660 1.8420538 50.609863 30-34
CACAG 6020705 1.8411531 16.291914 140-149
CCAGA 6019760 1.8408642 33.19264 170-179
CAGTT 4512200 1.8397738 25.100079 190-199
AGGAC 6241115 1.8344556 20.75748 220-229
TCAGC 5571770 1.8311944 11.958006 45-49
AGAGA 5371760 1.822627 27.55797 170-179
TTCCA 4258335 1.806398 22.365246 180-189
CAAGG 6113830 1.7970426 17.570396 80-89
GAGGA 6325200 1.7869885 26.844034 220-229
AGTCA 4679295 1.775243 29.293613 160-169
ACCTG 5344370 1.756458 31.012667 25-29
AGTTC 4306545 1.7559212 22.333694 190-199
ATACT 3575280 1.7507328 26.071978 130-139
GCCGA 6873510 1.7501991 19.029642 220-229
TAGTG 4462935 1.749037 25.840399 120-129
TACAA 3834370 1.7470493 17.810171 130-139
TTCTG 3982560 1.7451673 43.950966 30-34
TAGTA 3670830 1.7277323 16.63584 120-129
TGACC 5247035 1.7244685 14.5664215 210-219
CCTGG 6267160 1.7150561 33.77168 2
CTGAA 4519025 1.7144396 23.39285 200-209
CTCAG 5190490 1.7058845 80.485115 6
GCAAA 4814560 1.6995556 31.26927 200-209
GGAGC 6943135 1.6992877 13.564431 200-209
CATGA 4467230 1.6947893 22.670597 60-69
AGCTA 4442695 1.6854812 25.589582 50-59
GGATG 5549855 1.6851081 22.719791 100-109
ATGCA 4429650 1.6805321 16.208838 60-69
GCCGT 6056000 1.6572707 29.998423 230-239
ATTGG 4205725 1.6482357 20.889778 100-109
GTGCA 5207265 1.6449525 49.108154 25-29
AACAC 4470975 1.6420205 22.005499 190-199
CAGCT 4934965 1.6219047 13.005972 50-59
TCTGA 3930585 1.6026301 21.867868 220-229
TCTCA 3763180 1.5963519 25.906368 100-109
CTCCC 5380345 1.5937229 14.095252 190-199
AGGGG 6751905 1.5883275 32.27776 90-99
TCTGC 4476570 1.5811946 32.815968 200-209
GTTCT 3580220 1.5688609 23.732185 190-199
GGGGC 7693730 1.5678864 28.159918 90-99
CGATT 3843360 1.5670655 34.63853 160-169
GGGCC 7374470 1.5635293 28.684885 2
GGCTT 4575625 1.553434 22.16266 25-29
GCACT 4724330 1.5526783 26.224627 25-29
TTCTC 3380810 1.5413208 22.479275 190-199
CGTCC 5395140 1.536059 19.231722 180-189
ACAAT 3368105 1.5346054 22.505291 180-189
GAGCA 5210405 1.5314983 7.9066243 200-209
AGTGA 4182820 1.5252779 88.61439 9
CGGCC 6895405 1.5210118 31.936916 230-239
CCATG 4619985 1.5183847 15.838612 160-169
AGTTA 3225345 1.518058 16.310059 50-59
CATCA 3823840 1.509297 32.080452 45-49
CTGGT 4374810 1.4852569 32.23122 35-39
GTTAC 3595150 1.465862 16.030563 50-59
GTCCG 5352450 1.4647388 33.61963 70-79
CTTTA 2757980 1.4514415 41.49546 45-49
TTAGT 2866940 1.4502051 24.897932 110-119
CGTGT 4239965 1.4394768 24.81492 230-239
GTGAC 4529015 1.4306962 15.347125 210-219
TACGC 4352355 1.4304266 24.317167 140-149
ACTCC 4161705 1.4230173 16.795738 140-149
GGCAG 5805975 1.4209753 13.971484 150-159
GTCCA 4271985 1.4040127 29.077505 180-189
CTCAA 3555835 1.4035136 18.648476 150-159
AAGAG 4121840 1.3985317 19.02473 150-159
CAACC 4326960 1.3766485 15.235884 140-149
GGGGT 5441695 1.3757725 116.44165 7
AACCC 4313835 1.3724728 20.271374 140-149
GGCCT 5014635 1.3722931 71.17046 3
AAGCT 3615895 1.3718076 16.971643 200-209
CAACT 3467675 1.3687161 17.8466 130-139
AGCAG 4653355 1.3677641 12.524366 50-59
TAGCA 3604095 1.3673309 11.534499 130-139
CGACA 4454265 1.3621305 16.605377 70-79
CTACG 4121760 1.3546402 16.436806 130-139
ATCTA 2750820 1.3470135 21.122015 110-119
GTGCG 5114670 1.3453262 17.717669 240-247
GAGAG 4743335 1.3400818 25.267242 220-229
ACAGT 3520140 1.3354799 12.633048 120-129
CACGT 4062980 1.3353217 16.732645 180-189
TTACA 2703215 1.3237025 9.786674 120-129
GAACT 3475835 1.3186713 14.175787 190-199
ACAGA 3734805 1.3183985 20.503891 140-149
ATACA 2891110 1.3172729 21.838749 35-39
TGTCC 3723740 1.3152833 54.98757 15-19
ACATG 3453450 1.3101789 14.928029 190-199
GTGGC 4976270 1.3089224 14.652557 40-44
CCATA 3316095 1.3088864 14.89545 160-169
TCAAG 3438600 1.304545 23.430721 150-159
TGGAA 3572530 1.3027338 18.847313 120-129
TGCGA 4123445 1.3025787 14.698599 240-247
TGTGT 3081545 1.2979132 15.058906 240-247
TTATT 1978695 1.2918797 20.224022 240-247
CAATT 2629095 1.2874076 23.629997 180-189
TCACT 3026015 1.2836444 13.078243 190-199
CCAGT 3900600 1.2819545 19.09711 190-199
AACCA 3485605 1.2801312 17.388798 190-199
CCTTT 2803960 1.2783332 47.175518 45-49
AGAGC 4347865 1.2779713 24.114832 220-229
CCTAC 3723435 1.273159 17.689249 190-199
AGACC 4144315 1.2673466 101.897385 9
TCCGT 3576520 1.2632828 13.532855 150-159
ATCAT 2575460 1.2611438 15.657549 110-119
ATTAT 2071665 1.2585316 9.35641 50-59
AATTC 2559475 1.2533162 23.580597 180-189
CTTCT 2742920 1.250505 23.65944 30-34
ACACA 3402420 1.2495805 7.703934 130-139
ATATT 2055850 1.2489239 25.503782 240-247
ACGTC 3793735 1.246833 18.550886 180-189
GATTG 3180605 1.2464882 24.080914 100-109
ACATA 2729895 1.2438188 20.377502 130-139
ATCCG 3730240 1.2259649 23.932768 70-79
GATCT 2996385 1.2217256 9.589255 220-229
TCGGA 3861825 1.219934 95.59958 5
AGTCG 3859890 1.2193227 16.257763 150-159
TGGAC 3850380 1.2163184 16.367514 80-89
CGGCT 4434385 1.2135034 20.866146 230-239
TGAGT 3093065 1.2121811 21.496323 90-99
TACCA 3027840 1.1951101 11.039013 45-49
GTCGA 3735365 1.1799859 12.62714 150-159
CTTCG 3338195 1.1791028 107.81191 3
AGATC 3101605 1.176695 12.8789425 210-219
GATCC 3559580 1.1698766 23.77405 70-79
GGGTG 4625545 1.1694329 14.998068 60-69
AAGGC 3973275 1.1678677 29.460463 25-29
GAAGT 3174905 1.1577388 13.932644 140-149
AGGCC 4542815 1.1567352 14.995874 2
ACGCA 3762420 1.1505618 20.651302 140-149
CGCTG 4180095 1.1439148 12.218071 190-199
ATTAG 2425700 1.1416929 24.578972 110-119
AGCTC 3470520 1.1406064 12.424573 210-219
GGACT 3605215 1.1388719 13.383062 90-99
TCGAG 3594835 1.135593 12.347753 150-159
ACAAG 3216595 1.1354686 17.854551 80-89
TTGGG 3475160 1.1340171 15.588063 100-109
GACCC 4265355 1.1299565 43.51373 10-14
ATTCC 2657020 1.1271156 21.83677 180-189
GTATA 2389000 1.1244195 10.048764 240-247
AGGTC 3553810 1.1226332 18.518593 10-14
TTCGG 3306130 1.1224378 103.634346 4
GCAAG 3809225 1.1196483 17.202084 25-29
GAACC 3660440 1.119376 10.546503 180-189
CCCCT 3773775 1.1178375 10.01706 70-79
GCCCC 4867175 1.1169848 12.449346 80-89
CTTGA 2725555 1.1112993 22.772932 90-99
GCTTC 3138075 1.1084173 27.960258 2
TTGAG 2826145 1.1075745 21.224108 90-99
CGCAG 4281155 1.0901088 16.19541 140-149
GCACC 4110685 1.088982 11.1527605 130-139
ATGGG 3571745 1.0844924 20.403896 100-109
CACAT 2736505 1.0801181 13.519757 130-139
GAAGC 3665025 1.0772636 13.241256 200-209
TACAG 2838065 1.0767125 9.38635 120-129
TAGAC 2837380 1.0764526 17.569698 170-179
CAGCA 3479220 1.0639582 12.961446 45-49
CCGAT 3226730 1.0604833 22.866314 160-169
TGGCT 3121880 1.0598845 32.269905 40-44
GGAGA 3750525 1.0595942 85.10323 7
ATCAA 2315725 1.0551109 12.3549795 110-119
ACACC 3300840 1.0501822 12.955707 40-44
CTATA 2142300 1.0490352 13.366679 50-59
GTAGA 2864725 1.0446306 17.219503 170-179
GGGAG 4433045 1.0428357 14.362309 5
ATGGT 2644175 1.0362598 9.331129 120-129
GCTCT 2924920 1.0331275 17.49978 210-219
CTCCG 3627535 1.032801 9.7649765 140-149
GACAG 3512490 1.0324289 15.997665 70-79
TAGTT 2030670 1.0271885 15.252397 50-59
ACCGC 3854545 1.0211265 11.177851 220-229
AAACT 2236880 1.0191869 17.48777 130-139
GAGCC 4001785 1.0189729 17.698536 220-229
ATGAG 2785740 1.0158285 19.490927 60-69
TGAGG 3327340 1.0102836 12.019832 220-229
CGTGA 3180055 1.0045658 20.269514 150-159
GTAGC 3158605 0.99778974 11.060541 50-59
ACTCA 2521275 0.9951653 12.021025 190-199
AAGGT 2718775 0.9914096 20.935085 15-19
TTTCA 1880990 0.9899081 7.070598 100-109
CGAGG 4041425 0.9891129 14.412384 220-229
ATGAC 2595595 0.98472357 11.10205 160-169
AGCCG 3861195 0.98317456 18.674734 220-229
CGAGT 3105050 0.9808719 15.100525 160-169
ACCAA 2664425 0.9785428 17.145023 130-139
TTATA 1607240 0.9763945 12.957777 110-119
GGTAT 2485300 0.9739963 9.344047 110-119
GGGAC 3923690 0.96029794 10.271521 90-99
AACTC 2428455 0.9585286 15.855058 190-199
CCGTC 3358790 0.9562864 10.192635 140-149
GATAC 2509470 0.95204926 23.76211 35-39
CAGAA 2695520 0.9515275 18.486252 140-149
GCCAA 3109140 0.9507862 14.457991 180-189
ATGAT 2019180 0.950358 8.734926 120-129
ACAGG 3226250 0.9482941 15.532067 70-79
CCAAC 2979370 0.9479045 14.241599 130-139
CGGAC 3712540 0.9453227 10.899885 220-229
TTTAG 1865810 0.9437963 29.41435 45-49
GCAGT 2980195 0.94143075 6.1947474 50-59
CCGAG 3693645 0.9405113 17.615639 220-229
AGCCC 3531345 0.9355062 11.526317 80-89
TACAC 2351625 0.9282032 22.133564 35-39
GCCAT 2814560 0.9250213 7.509523 60-69
CATAC 2339540 0.9234331 13.816011 130-139
TAACA 2025360 0.9228123 8.777116 120-129
AAGTT 1956460 0.9208378 17.990479 140-149
GAGAT 2524290 0.92049 12.473282 210-219
TATGG 2344535 0.91883 11.957203 35-39
CGTAT 2246210 0.91585445 7.335091 240-247
TTTAT 1400430 0.91433346 10.441256 240-247
AACTG 2407445 0.9133427 11.527207 60-69
CCCAG 3437495 0.91064376 14.850439 80-89
TGGTT 2137650 0.9003548 9.081319 50-59
AATCA 1975200 0.8999579 13.4766245 110-119
CCCCA 3257075 0.89770126 16.967821 80-89
GACTG 2831570 0.89448076 13.037703 90-99
AGAAG 2618630 0.8884957 16.217196 140-149
TCCTT 1941655 0.8852059 10.799788 1
TCATA 1801755 0.8822781 8.632658 110-119
CGGAG 3602670 0.8817305 74.00512 6
TATTT 1337285 0.8731064 10.668313 240-247
GATCA 2297485 0.87162584 9.512618 110-119
GTTAT 1714635 0.8673263 10.473375 110-119
ATCCA 2190130 0.86446 10.1488 180-189
GCCTA 2624175 0.86245024 17.060045 190-199
CATGG 2716125 0.8580122 8.839272 200-209
AGTCT 2099830 0.8561704 15.549768 8
TTACC 2012380 0.8536574 9.810337 45-49
CTATC 2007405 0.851547 8.50602 110-119
ACGCT 2576345 0.84673065 14.924613 190-199
CATTA 1726235 0.8452977 12.023498 110-119
ATAGT 1793260 0.8440253 15.359421 120-129
TCTTC 1849480 0.84318316 47.87093 2
GTCTG 2481995 0.8426423 14.402928 3
GCTTG 2475240 0.84034896 17.48907 90-99
GGTAG 2763375 0.83904636 11.888969 120-129
GATGA 2297335 0.8377301 6.426511 60-69
TCATC 1956950 0.83014387 10.836287 110-119
GGTGC 3150180 0.8286009 12.002606 60-69
ACAAA 1947185 0.825505 12.954037 130-139
GTCTA 2024280 0.8253662 9.571251 30-34
ATATG 1742035 0.8199154 5.9662833 50-59
GGTTC 2409070 0.8178841 11.140361 160-169
ACATC 2066185 0.815538 5.759792 110-119
TGGCA 2565600 0.810462 5.654741 100-109
CCTCC 2730855 0.80891216 10.953119 140-149
ATATA 1409080 0.7964922 10.367598 130-139
TTATG 1567360 0.7928291 11.105313 50-59
TGATG 1971265 0.77254444 11.727038 120-129
TATAC 1570450 0.76901335 8.69201 130-139
CTGAC 2325760 0.76437455 9.737954 220-229
GGGAT 2495800 0.75780225 13.5929 100-109
CATCC 2188645 0.74836624 6.906026 110-119
CCAGC 2820615 0.7472231 6.2026925 80-89
CCCCC 3112635 0.7431836 13.538475 80-89
GTTCA 1819530 0.7418828 13.45884 160-169
TCAAC 1876980 0.7408574 7.7322335 110-119
GGTTA 1882590 0.73779255 8.020344 50-59
ATCTT 1401880 0.73776704 13.006917 1
CGCCG 3336590 0.73599625 10.94684 220-229
GCGGA 2996885 0.7334684 9.151314 220-229
GACCG 2869020 0.7305374 9.458386 210-219
GATTA 1539545 0.72461045 5.842596 160-169
TTTAC 1374455 0.7233341 13.319762 45-49
TTGGA 1836520 0.7197376 6.424002 60-69
GGGCA 2940595 0.71969175 13.950597 150-159
TCCGG 2622340 0.7176233 11.987265 70-79
CACAA 1943080 0.71362 8.459288 130-139
ATAAA 1350900 0.7105105 7.5426717 130-139
GACCA 2318415 0.7089798 6.136988 170-179
TATTG 1395625 0.70595914 6.641919 240-247
ACGCC 2663070 0.70548713 12.73337 180-189
AACGC 2303500 0.7044187 14.158962 180-189
GGATA 1923230 0.70131165 15.621158 35-39
CAAAC 1900785 0.6980866 14.081792 130-139
ATGCC 2107250 0.6925599 7.9590597 50-59
TGCCA 2105910 0.69211954 7.204715 60-69
CAACG 2248910 0.6877249 14.423403 180-189
AATAC 1501935 0.68432474 11.014668 130-139
TTTCT 1209900 0.6843157 9.649694 4
TATAG 1453520 0.68412143 6.491963 120-129
AAATA 1295495 0.68136996 12.221775 130-139
GAAAT 1537205 0.6732019 6.982188 110-119
CAGTC 2044480 0.67193013 5.3862853 210-219
GCGAC 2617075 0.6663848 7.328872 70-79
GCCGC 3015780 0.6652309 12.085505 220-229
AGGCA 2247950 0.6607416 6.441618 80-89
GACGA 2226900 0.65455437 9.961961 220-229
TCCAC 1903215 0.65076876 10.731506 180-189
CCCGT 2274205 0.64749247 9.935632 140-149
AATAA 1228660 0.6462179 7.0560675 130-139
CACGC 2425070 0.6424373 12.086365 190-199
ATTAA 1136525 0.64242864 6.312774 110-119
ACTTC 1503475 0.63777846 30.203348 4
TACTT 1207240 0.6353339 11.684643 1
AGATG 1725155 0.6290829 5.5680647 120-129
CCGGC 2850050 0.6286736 9.7237625 70-79
CACGA 2053630 0.6280074 9.140125 180-189
CCCTA 1822680 0.62323135 12.206329 110-119
CACCG 2338465 0.6194943 9.183361 45-49
TCAAT 1253770 0.61394244 8.079844 110-119
GGCCC 2774085 0.6119171 10.680432 70-79
CCGTA 1851230 0.60841745 6.300388 230-239
AAGCA 1712320 0.60445464 5.6830583 120-129
AATGG 1633095 0.595513 6.7239747 120-129
ACGAC 1941515 0.59372234 9.313874 220-229
CTTTG 1353550 0.5931288 7.085033 45-49
TAGCT 1447780 0.59030795 12.098872 50-59
GGTGA 1936690 0.58803916 6.3698125 120-129
AGTAA 1337315 0.5856623 5.000906 50-59
GAAGA 1724930 0.58526516 6.299683 15-19
TGATT 1150105 0.581766 5.973382 50-59
GTTCC 1638855 0.5788692 6.960956 140-149
GGGGA 2442325 0.57453597 9.150031 4
ACCTA 1440080 0.56840986 7.1143565 130-139
ACCCG 2133655 0.5652371 8.543482 140-149
CGGCA 2203095 0.5609732 11.172352 70-79
TCAGG 1763720 0.55715156 5.489003 150-159
ATTTC 1055225 0.55533296 6.2091665 240-247
CCACG 2094190 0.5547822 8.051802 180-189
TAATA 975995 0.55168796 7.7119327 130-139
TTAGC 1350410 0.55060697 9.499458 45-49
GTCCT 1555590 0.54945874 6.124305 45-49
CGGTT 1611365 0.54706174 11.013835 160-169
GGAGG 2320995 0.5459941 5.3739953 6
CTGCG 1989490 0.54443914 11.555697 25-29
CCTAT 1279290 0.54267853 5.458505 110-119
TTCAA 1108215 0.54266745 5.2912936 200-209
AAGAT 1235180 0.5409334 5.7698956 15-19
GCTAC 1635130 0.537395 8.027304 50-59
ACCCC 1919730 0.52910787 9.326913 140-149
CTGTT 1197010 0.5245326 5.7526855 240-247
AAATC 1143655 0.5210821 7.50228 110-119
TATGT 1015550 0.51370305 6.5006967 140-149
ACATT 1030190 0.50446045 5.9777355 110-119
AGTTT 993850 0.5027264 5.0550423 140-149
TGACG 1584810 0.50063473 8.873363 220-229
GGGTA 1645125 0.49951097 5.3953733 100-109
GGGTT 1498160 0.4888808 5.693863 100-109
AGTAT 1033125 0.4862561 5.0791636 110-119
GTAAC 1281150 0.48604602 6.9116635 120-129
CGCGG 2286210 0.48472044 6.4327955 220-229
TATCC 1141515 0.48423398 5.533834 110-119
TGGCC 1722415 0.47135198 15.554687 2
AACAT 1025380 0.4671926 5.5507803 110-119
ATGCT 1139260 0.4645141 7.3026266 50-59
GGTTT 1082575 0.4559688 5.299852 100-109
CAATC 1153380 0.45524734 6.069742 110-119
TAAAT 803740 0.45431963 7.5720477 130-139
TGCTA 1113330 0.4539416 6.5388517 50-59
CCGGT 1653380 0.45246008 8.556024 160-169
CATAA 992780 0.4523391 5.706241 110-119
CCGCG 2022870 0.44621146 6.6475954 220-229
TTGGT 1057610 0.4454538 5.5291576 120-129
TGCGC 1577980 0.4318263 8.566395 25-29
ATTCT 819715 0.4313912 7.047298 2
GGTCA 1363480 0.4307175 5.509963 160-169
GCCTT 1213545 0.42864305 9.03089 1
ACGAG 1434805 0.42173332 8.019469 180-189
GCATG 1309575 0.4136891 8.117144 60-69
GCGCT 1504130 0.41161665 7.770469 25-29
CTAAC 1029740 0.40644574 5.5565376 110-119
CACTT 955155 0.40517953 6.651131 3
ATGGC 1270165 0.4012396 7.4621153 1
CGGTG 1517390 0.39912343 5.0765843 8
CCTAA 1009435 0.3984312 7.6233926 110-119
CCGGG 1854620 0.39321506 5.30924 80-89
AGCTT 962610 0.39248806 7.491454 1
GAGGC 1579710 0.3866239 6.19647 35-39
CATGC 1168070 0.38389295 6.7417355 60-69
GGTAA 1049415 0.38267237 7.277138 120-129
TTTGA 754005 0.38140383 5.8982415 45-49
TTCTT 649080 0.36711767 20.231958 1
CGAGC 1368815 0.3485408 6.7955813 180-189
TGGGC 1320440 0.34731907 9.812489 1
TGCTG 1016015 0.34493914 13.935479 1
CTCGG 1234130 0.33772907 5.4864135 6
CTCTT 696765 0.3176571 9.572091 1
GCGAG 1258130 0.3079193 5.5641036 240-247
GCTTT 701015 0.30718642 5.1872177 45-49
CGCTT 860855 0.30406743 6.0395007 1
TCACA 760955 0.3003544 5.0479317 7
CGGGA 1222795 0.29927126 5.087206 80-89
AACTT 606605 0.29704058 10.934975 3
GACTT 728180 0.29690316 6.8689294 1
ATTGT 585450 0.29614243 5.472141 240-247
TTCCT 621710 0.28343934 6.6169844 4
GAGGT 907425 0.27552238 6.77836 7
CCCTT 731515 0.26881948 5.5698 3
GTCTT 603720 0.26455155 11.866892 1
TGTAA 558850 0.26303133 7.9150887 25-29
TCGGT 772515 0.26227042 6.5747256 7
AATCT 530555 0.2598006 7.513567 2
TTGGC 762745 0.25895345 7.690536 1
TAACT 525710 0.25742814 6.765356 2
CGGGG 1238445 0.25237966 9.128993 3
CCTCG 864800 0.24621855 5.9966674 5
TGCTT 509280 0.2231677 11.343456 1
TCGCA 553130 0.18178938 6.914794 7
TAGGC 484225 0.15296458 7.1241875 1
TTGCT 290555 0.12732188 5.8539796 4
Produced by FastQC (version 0.10.1)