FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_1_SL248338.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_1_SL248338.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6705845
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7835990 8.890725 61.385227 110-119
ATACA 7263130 7.778058 37.743977 110-119
CACAG 10532510 7.6359167 42.587513 110-119
TCCCT 12084595 6.968971 105.842865 30-34
CAGTA 8683630 6.670011 39.336636 110-119
AGTAA 6810525 6.555031 48.23736 110-119
GGAGA 10839380 6.347859 97.0492 35-39
GTAAT 7386985 6.0115767 39.890793 110-119
GGTGA 11990785 5.937417 102.9864 45-49
CCCTG 11793385 5.769337 69.668976 30-34
AATAC 5229095 5.5998178 40.078842 110-119
GTAGT 9561740 5.5813074 33.021416 80-89
AGACG 8316790 5.4191585 103.9633 40-44
ACAGT 6953030 5.3407145 35.79409 110-119
GTGAC 9244765 5.093297 85.95881 45-49
TAGTA 6165350 5.0174026 42.812214 80-89
GAGAC 7363240 4.7978325 144.10298 40-44
CCCCA 7437020 4.787542 101.82293 60-69
CTCAG 7706040 4.7237577 34.91294 140-149
ACTCC 6766290 4.614877 119.119675 30-34
AGTAG 6442040 4.44729 34.607006 80-89
CGTGT 9453415 4.403712 23.088371 120-129
GGCCC 10605445 4.4011602 58.326965 60-69
GCACA 6066955 4.3984547 34.470325 110-119
ACCAG 6053105 4.3884134 91.48867 45-49
GTGTC 9330355 4.346386 23.1956 120-129
TGACC 6987000 4.2829905 146.46729 45-49
CACTC 6266815 4.2742157 98.260574 25-29
CTCCC 7670935 4.175312 68.43459 30-34
CCCAG 6922135 4.004981 87.76502 60-69
ATGCA 5209345 4.0013666 96.50702 25-29
TGAGG 7837355 3.8807836 70.59502 35-39
GACCA 5343195 3.8737383 104.962074 45-49
TACAC 4486785 3.834544 27.766798 110-119
ATGGT 6475800 3.7800057 25.842049 190-199
AGATA 3910255 3.7635636 19.805195 150-159
CCCTT 6522930 3.7616577 36.796062 20-24
AGGAG 6417145 3.7580688 97.44046 35-39
CCACT 5506535 3.7556746 28.242208 240-247
GACGG 7941980 3.7117848 110.477135 40-44
ACGGT 6685585 3.6833463 98.45279 40-44
CTGAG 6615635 3.6448083 106.14479 35-39
CAGGG 7738165 3.6165292 34.628887 50-59
GAGGA 6083715 3.562802 127.72092 35-39
TGTCC 6856015 3.5534902 20.10789 120-129
TGGTG 8379550 3.5083072 17.958155 190-199
TGCAC 5656895 3.4676433 115.47008 25-29
GCCCC 7455935 3.4426556 72.99813 60-69
TCTTG 6267095 3.4414828 18.453062 170-179
CTTGG 7344510 3.4213145 21.217464 20-24
CCGTG 7754340 3.4094086 23.17286 120-129
GGGTT 8100105 3.3913107 40.703117 50-59
CCTGA 5486510 3.3631988 85.155464 30-34
CTCTC 5773655 3.3295646 13.743628 130-139
AGGCT 6035365 3.3251152 26.416775 30-34
GGATG 6710115 3.3226137 70.83166 20-24
CAGAT 4288610 3.294138 18.181417 150-159
CTTCA 4553770 3.2906113 16.737371 200-209
TCAGG 5938530 3.271765 20.905329 140-149
GCACT 5281905 3.237777 89.56279 25-29
TCGCA 5255740 3.221738 24.08787 100-109
TGTCT 5786960 3.1778238 20.051954 180-189
CCAGG 6095865 3.1698778 38.994083 50-59
CGCAC 5414420 3.1326535 24.535172 100-109
TCTCA 4313310 3.1168518 21.554413 130-139
ACAGA 3412955 3.100479 14.824863 140-149
GGTTG 7268930 3.0433187 73.33932 15-19
CGGAT 5480775 3.01957 80.05113 20-24
CCTGG 6859780 3.0160906 28.300072 50-59
ACCAC 3717895 2.9990218 19.800522 230-239
GACTC 4802635 2.9439874 19.699091 190-199
GATGC 5260780 2.8983662 95.873215 25-29
GCCCT 5903125 2.887815 19.784035 20-24
GGTTC 6160090 2.8695726 45.73675 50-59
TTCCC 4963465 2.862342 47.31907 50-59
TGGCC 6442225 2.8325012 42.772953 60-69
TTCTT 4355315 2.8193436 21.105 170-179
TCTCT 4613105 2.8185508 14.763176 180-189
GTTGG 6723355 2.8149002 52.985657 15-19
AGGGT 5684150 2.814592 48.866077 50-59
CGGCC 6673685 2.7695167 12.849108 120-129
GATGG 5588290 2.7671254 20.737148 15-19
GCCGT 6262425 2.753447 21.925966 120-129
TCACA 3216825 2.7491975 12.027473 140-149
CGGTG 6926815 2.7372508 69.38836 40-44
TCAAA 2550805 2.7316473 56.562164 70-79
TCCAT 3652865 2.639606 20.91117 70-79
AGATG 3822110 2.6386101 17.02412 180-189
AGGGA 4488340 2.6285036 9.224037 150-159
CCAGT 4189435 2.5680993 34.62217 60-69
AGTCA 3334790 2.5614963 30.11269 70-79
TGGAG 5164855 2.557455 18.06568 25-29
GTTCC 4921335 2.5507405 51.514343 50-59
TGTTC 4595640 2.523628 12.9032755 140-149
ACAGC 3476060 2.5200927 11.31555 120-129
GCGGA 5348105 2.4995046 87.814186 20-24
GGCTG 6292215 2.4864776 13.708908 30-34
ACTCT 3423100 2.4735749 13.713951 200-209
TCCTC 4279465 2.4678917 16.646906 130-139
GTTCT 4433190 2.4344208 17.827234 160-169
TACCA 2848365 2.4343002 16.506 230-239
GAGAT 3510355 2.4233887 16.569723 180-189
GGCGG 7219355 2.4200828 42.330364 20-24
TGTAG 4081260 2.3822827 8.241934 210-219
CAGCT 3882810 2.3801396 12.466119 150-159
CACGG 4564050 2.3733268 9.87578 120-129
ACTAC 2766800 2.364592 13.769108 230-239
CTACT 3253265 2.35085 8.341057 230-239
TTGGG 5595585 2.342731 79.749306 15-19
CTGGA 4239300 2.3355937 14.609191 180-189
AATAA 1726120 2.3162599 9.6343565 110-119
CATTT 2993785 2.292039 17.837694 150-159
CAGAG 3479655 2.267317 9.653194 140-149
TCATT 2954565 2.2620125 16.666424 150-159
TTCAT 2954220 2.261748 15.950204 140-149
CACTA 2637735 2.2542892 15.927126 240-247
GATAC 2934835 2.2542856 14.94804 150-159
GTGAA 3259390 2.250134 18.00594 190-199
CTGGC 5021710 2.207933 33.14581 60-69
TCAGC 3590360 2.2008698 14.402586 140-149
TTCAC 3033070 2.1917343 18.197906 200-209
TTGTC 3949840 2.1689963 13.073428 170-179
ATTTG 3128550 2.1527388 15.741637 150-159
CTCGC 4345965 2.126051 16.381813 100-109
ATGTA 2610535 2.1244705 9.363567 70-79
ACACA 2088380 2.1108673 14.137055 110-119
TACAG 2741440 2.1057363 14.302122 160-169
TTGGA 3594330 2.0980558 15.988726 170-179
AGCTC 3406295 2.088039 12.573912 140-149
GATAT 2559520 2.0829542 8.783729 180-189
TGGGG 5862020 2.0819728 52.893803 15-19
GAACT 2685690 2.0629141 11.160615 160-169
TCTGC 3975600 2.0605636 11.854249 210-219
CTCTG 3970745 2.0580475 14.364643 180-189
TACTA 2254095 2.0410168 11.156011 230-239
TCTCG 3937770 2.0409563 15.739054 100-109
GAATC 2655305 2.039575 14.250245 190-199
ATATA 1795920 2.0376534 10.4438715 110-119
GCTGT 4359735 2.030908 10.251857 140-149
ATATG 2490660 2.0269156 9.061203 180-189
GTCTC 3905370 2.0241632 11.818809 180-189
GTTCA 3108660 2.0189495 14.750456 140-149
GTCCT 3873145 2.007461 14.169721 130-139
TAGTC 3089300 2.0063763 25.05588 70-79
ACGGC 3855615 2.0049374 14.67663 120-129
CAGGC 3855555 2.0049062 15.179266 140-149
TGAAT 2461310 2.00303 13.806757 190-199
AATCG 2597635 1.9952778 13.156844 190-199
TTTGC 3629275 1.9929628 12.129703 150-159
AACCA 1959840 1.9809434 15.084229 70-79
TTGCA 3037000 1.9724094 14.297702 150-159
ACACG 2716055 1.9691005 13.821185 120-129
GACGT 3569970 1.9668341 10.028901 60-69
AATAT 1729155 1.9619019 13.17909 110-119
ATCTC 2700055 1.9510936 7.704772 100-109
CCAGA 2690000 1.9502112 30.911097 60-69
TGGAA 2799950 1.9329575 9.243552 170-179
CCTTC 3328810 1.9196658 13.748762 200-209
CAGCC 3312640 1.9166138 10.266854 120-129
TCGGC 4347455 1.9114782 8.0429945 130-139
GAGTC 3454480 1.903206 12.316761 210-219
AGTAT 2336305 1.9013003 12.545528 220-229
GTCAA 2470135 1.8973434 38.31109 70-79
CATAG 2457915 1.887957 8.279431 210-219
AGAAC 2039775 1.8530217 11.891832 160-169
GGGGC 5525630 1.8523098 48.187927 15-19
GTTGT 3720905 1.8364313 7.161916 210-219
CGTAG 3305035 1.8208711 9.687987 210-219
CTGTT 3302395 1.8134617 12.700302 140-149
GGGCG 5377785 1.802749 58.839233 20-24
GCAGA 2730190 1.7789714 14.657438 150-159
ATTGT 2554970 1.7580614 8.371324 50-59
CCTTG 3370245 1.7468067 24.004795 20-24
AGTCT 2657955 1.7262348 13.022641 210-219
GGTCA 3129795 1.7243247 7.7965226 130-139
TGACT 2653675 1.7234552 11.857348 190-199
GAGAA 2103330 1.7173246 11.079504 160-169
AACTG 2222835 1.707389 10.956006 160-169
TGCAG 3078950 1.6963121 13.188759 150-159
GTCAC 2763755 1.6941656 8.763341 140-149
ATAGT 2073690 1.6875825 7.3140206 220-229
TCCCA 2471995 1.6859983 11.053244 240-247
CACCA 2088500 1.6846783 12.809027 230-239
GTCCC 3438235 1.6819881 23.050322 50-59
ACTGA 2186180 1.6792338 9.175163 180-189
CAAAA 1318055 1.669375 22.378271 70-79
TGGTT 3375260 1.66584 18.167574 10-14
TTGTA 2378435 1.6365886 9.142392 210-219
CTCCA 2399420 1.6364994 7.321911 150-159
AATAG 1688070 1.6247429 10.107575 240-247
TTGGT 3290975 1.6242418 25.336916 25-29
GAACC 2238540 1.622909 12.496511 70-79
TAGTT 2345295 1.6137851 9.952513 220-229
GTGCT 3458610 1.6111342 8.411123 220-229
GGACG 3441130 1.6082556 7.359815 170-179
GCTCA 2609580 1.5996573 11.229432 140-149
AAAAA 1007345 1.598702 8.721282 8
TGTGC 3418870 1.5926222 8.048594 220-229
GCTCC 3254360 1.5920365 5.9805717 150-159
CTGGT 3414875 1.5907612 7.04401 160-169
TTCAG 2438545 1.5837369 9.555194 150-159
GTCTG 3398280 1.5830306 8.984272 210-219
TCTGG 3383775 1.5762737 11.737035 180-189
TCAGA 2049695 1.5743979 9.787236 130-139
ATAGA 1625640 1.5646548 10.463936 240-247
GGCCG 4194715 1.5645431 12.970384 120-129
CAGAC 2158025 1.5645366 19.059853 60-69
TGCTC 3006295 1.5581704 5.042152 150-159
ACCAT 1821380 1.5566072 12.8694315 45-49
CTGTG 3338335 1.5551064 5.1767845 210-219
CCCAC 2392545 1.5401881 10.727235 240-247
CCACC 2366020 1.5231128 9.489613 230-239
GCTCT 2907335 1.5068793 10.156937 130-139
TGATA 1846930 1.5030438 8.2250595 180-189
CAAAG 1643425 1.4929599 27.635944 70-79
CCTCG 3044280 1.4892652 9.61184 130-139
CGACT 2398925 1.470527 8.693272 190-199
ACCGT 2374950 1.4558303 22.554865 45-49
CATGT 2240550 1.4551471 9.310468 70-79
GCTGA 2616575 1.441572 22.513954 30-34
TCGAC 2350355 1.4407539 9.358898 190-199
CTCTT 2332130 1.4249032 8.427663 200-209
AATTG 1745700 1.4206622 9.360395 170-179
AAATA 1051260 1.4106734 5.1707506 9
TATAC 1557340 1.4101257 6.9328604 110-119
TGGAC 2557030 1.4087663 10.257993 170-179
CACTG 2296770 1.4079067 7.3222976 230-239
GAGGG 3349995 1.4071646 8.333482 200-209
GGGAG 3340050 1.4029874 6.664504 150-159
TATGG 2394530 1.3977175 6.5904436 180-189
GCGTA 2491650 1.372746 10.136344 210-219
CTCGA 2230565 1.3673234 8.452925 190-199
ACGGA 2088345 1.3607501 9.318781 200-209
CTGAT 2094765 1.3604658 7.6738687 180-189
GTCCA 2194905 1.3454639 19.51651 70-79
CTTGA 2065470 1.3414397 8.705687 200-209
GTGGA 2686500 1.3302606 22.85531 25-29
GAATT 1632690 1.3286939 10.362272 170-179
CCTCA 1943865 1.3257928 6.9856462 130-139
CCATG 2160985 1.3246713 9.4045105 70-79
ACGTG 2391295 1.3174565 9.502034 170-179
CCATC 1930955 1.3169876 10.584628 240-247
ACTTC 1801805 1.3020068 6.5079856 220-229
AGCCG 2494775 1.2972943 9.09348 120-129
GTGTT 2624640 1.2953758 7.505342 160-169
ACTAT 1430245 1.2950449 6.6989965 230-239
ACTCG 2105470 1.2906408 8.761326 190-199
TTGGC 2761875 1.2865722 9.275514 60-69
GGAGG 3061415 1.2859466 17.251638 30-34
TCCAC 1883115 1.2843589 9.719846 240-247
GTGGT 3066950 1.284055 12.866274 50-59
AGATC 1668180 1.2813512 6.840982 130-139
GGCTC 2892325 1.2716902 8.507019 130-139
TGGTC 2729115 1.2713115 14.482422 50-59
CTGCT 2451190 1.2704581 5.618829 90-99
ACTGG 2274355 1.2530297 7.3293314 160-169
TTGAG 2143150 1.2509835 8.372429 200-209
ATAAC 1163385 1.2458645 7.365045 80-89
ATCGG 2259895 1.2450631 7.8624096 190-199
GGAGT 2514160 1.244924 7.023635 210-219
GATCT 1907020 1.2385328 5.4704704 130-139
AGCTT 1904875 1.2371397 6.6903124 150-159
GGAAT 1779365 1.2283922 8.627935 170-179
AAGTA 1274425 1.2266155 15.694791 70-79
ATACC 1422980 1.2161224 8.964266 70-79
TGCCC 2484880 1.2156059 8.143281 60-69
CCATA 1413255 1.2078111 12.266514 70-79
TCTAC 1655820 1.1965163 7.7937255 180-189
AGAGC 1822095 1.1872635 7.445114 140-149
TCACG 1934600 1.1858985 9.313391 200-209
ATAAA 875585 1.1749371 5.7319837 7
GTCTA 1797510 1.1674105 7.857131 180-189
GCATA 1515135 1.1637954 7.5884123 210-219
AAAGT 1201075 1.1560172 20.172106 70-79
TTCCA 1579960 1.141699 7.4414496 230-239
TACTG 1749035 1.1359279 6.8817058 180-189
AAAAG 995935 1.1337 15.442531 70-79
ACTGT 1745105 1.1333755 5.529498 230-239
GCTTC 2185970 1.1329939 5.8813057 150-159
GAGCT 2042805 1.12546 6.6489773 140-149
TATCA 1234135 1.1174731 7.3537455 70-79
AGTTC 1711135 1.1113135 5.477836 160-169
AGGTT 1885620 1.1006601 23.515446 10-14
CTACC 1610115 1.0981622 8.834601 230-239
CTCGG 2490850 1.0951706 8.342063 130-139
CGGCT 2487745 1.0938054 7.9402094 130-139
ATCCA 1273635 1.0884875 15.354968 240-247
TATGT 1579260 1.0866805 7.773794 220-229
CAATA 1011825 1.0835595 6.445057 100-109
TCTGT 1970835 1.082255 6.1162863 210-219
ATGGG 2180650 1.0797814 32.62049 15-19
CTGCG 2447255 1.0760028 6.9871135 210-219
AGAGA 1317275 1.0755274 14.250782 35-39
ATATC 1165675 1.0554845 7.8437343 70-79
GAAGA 1292325 1.0551562 19.028198 35-39
GTCCG 2343435 1.0303555 5.150309 130-139
TAGAT 1258040 1.0238012 7.972868 240-247
GCGGT 2588380 1.0228431 5.897348 130-139
AACTT 1127540 1.0209545 5.724518 200-209
CTGCC 2072590 1.0139134 5.4573326 200-209
ATACG 1319980 1.0138941 5.424249 120-129
TGCGT 2175960 1.0136337 8.169126 210-219
CTGAA 1316645 1.0113325 15.494367 35-39
TATAA 890850 1.0107597 6.716858 240-247
TCCCC 1850865 1.0074311 5.36041 80-89
GGGGT 2822570 1.0024726 7.6372566 10-14
CGAAC 1377250 0.9984863 13.121789 70-79
CATGG 1805695 0.99482685 5.23893 190-199
CGTGG 2502435 0.9888805 8.883322 50-59
AAAAT 734725 0.98591876 5.317155 8
ACTAA 915660 0.9805768 12.953424 240-247
ACGTC 1581005 0.96914685 13.445072 70-79
CAGCG 1858220 0.96628284 6.425512 160-169
CGGAG 2063820 0.96455234 6.6064196 210-219
CCCAA 1188885 0.95900834 10.109045 240-247
AGCTG 1729740 0.95298034 5.126589 90-99
CTTCT 1555985 0.9506878 5.220342 210-219
ACCCA 1170760 0.9443878 12.74793 240-247
TGTAA 1158535 0.94282335 6.8258348 230-239
GAGTT 1605695 0.9372643 5.3043385 160-169
CCATT 1293785 0.93490523 6.312378 50-59
ACAAT 869135 0.93075335 7.484672 100-109
AAGAG 1123975 0.917702 14.233843 35-39
TGAAG 1316735 0.9090137 10.340096 35-39
TAGGC 1643760 0.9056106 5.480942 160-169
CATCA 1044330 0.89251643 5.318851 230-239
TCGAA 1161390 0.89207906 16.934824 70-79
TGCAT 1370075 0.8898086 5.8653693 210-219
GGTGG 2488190 0.8837132 22.646374 25-29
CGGTC 1994590 0.8769764 5.6719975 130-139
ATCAA 814160 0.8718808 13.959627 70-79
CGTCC 1771430 0.86658555 10.932851 70-79
CCAAT 1013800 0.86642456 10.693254 240-247
GAGGC 1843470 0.86156887 14.61002 30-34
GACCG 1631675 0.8484784 20.574354 45-49
TGAAC 1104310 0.84823513 6.541049 190-199
CCCCC 1639180 0.8421149 5.019657 80-89
AGCGT 1526095 0.84078455 6.5544467 160-169
GTATG 1425320 0.8319773 5.444011 220-229
TTCTG 1514760 0.83180803 5.075216 210-219
GGTCC 1877235 0.825378 14.517322 50-59
GGGCC 2206290 0.8229011 17.132528 15-19
CCAGC 1415170 0.8187834 5.533603 80-89
AACCG 1128230 0.8179505 5.7811975 190-199
CTTCC 1413205 0.81497014 5.3472104 230-239
GGTGC 2007180 0.7931719 5.0093727 220-229
TAACC 921265 0.7873413 5.85964 80-89
CATAC 912510 0.779859 10.764465 70-79
TGGGC 1967130 0.7773454 18.61979 15-19
CTAAT 851510 0.7710174 9.803541 240-247
AATCC 871380 0.74470806 9.57876 240-247
ATGAT 915070 0.74468994 6.748641 240-247
AAAGA 648135 0.73778975 5.8041086 8
AAAGG 896310 0.7318183 9.073613 10-14
TGAGA 1048830 0.7240643 5.766461 240-247
GTAGC 1305365 0.7191758 5.665794 80-89
CCTTT 1173700 0.7171164 5.5412264 50-59
AGCCA 986180 0.7149661 5.7398176 80-89
AAAGC 781700 0.7101308 10.500472 70-79
GTCGA 1277630 0.7038955 11.678344 70-79
TAGGT 1173195 0.6848086 5.475082 10-14
AAGGT 989460 0.683078 10.096249 10-14
TATGA 835430 0.6798784 5.9680915 240-247
CATTG 1041810 0.6766137 5.7772946 50-59
CCGTA 1082280 0.66343135 5.7075987 80-89
CAATC 766505 0.65507865 9.343748 240-247
GAGGT 1276810 0.63223153 6.880387 10-14
AGGAT 906425 0.6257544 6.2252216 10-14
CGATG 1119440 0.6167426 14.2846985 15-19
GGAAG 1040315 0.60923904 6.1923356 30-34
AGAAG 738760 0.6031821 5.5461345 9
AGACC 829280 0.60121596 5.017619 240-247
ATAGG 866195 0.59798145 5.2824864 10-14
CATCC 851585 0.58081466 7.2135363 240-247
AATGG 829645 0.572749 6.7179875 10-14
TAAAA 420755 0.56460613 6.200585 8
GGGTC 1385375 0.5474549 5.562085 70-79
AAGCA 599160 0.5443034 7.094401 70-79
AAGAA 470140 0.5351732 5.3437696 9
AAATG 545360 0.5249011 5.3563385 9
ACGAT 674255 0.51790416 6.5686088 10-14
GAAGG 866115 0.5072224 5.5895376 10-14
GAAAA 445235 0.5068232 6.147181 8
AAGGG 863825 0.5058813 6.334877 10-14
TTGCC 960840 0.49800584 5.7772784 60-69
GGTCG 1208300 0.47748065 5.6486077 70-79
TACCG 755435 0.46307725 5.7200418 70-79
ATAAG 480600 0.46257052 5.805672 9
TAAGG 623230 0.4302495 5.9677553 10-14
CGGTT 914120 0.42582718 8.093807 10-14
GAAGC 643130 0.4190587 5.0011206 30-34
AAGCT 531350 0.408137 8.390895 30-34
TAAAG 400290 0.3852733 5.4312277 9
Produced by FastQC (version 0.10.1)