FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_10_SL248339.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_10_SL248339.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7792061
Filtered Sequences 0
Sequence length 251
%GC 54

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 8792070 8.188282 57.99453 110-119
CACAG 11825685 7.2302856 40.389824 110-119
TCCCT 13865120 7.104607 108.27998 30-34
ATACA 8059680 7.102029 36.16382 110-119
CAGTA 10046760 6.4922047 38.931686 60-69
CCCTG 13959850 6.0569887 70.867775 30-34
AGTAA 7627600 6.0151877 45.293354 110-119
GGAGA 12208070 5.978186 90.07504 35-39
GGTGA 13578170 5.75845 99.96455 45-49
GTAAT 8299485 5.6683207 38.370857 110-119
AATAC 5890345 5.1904545 37.920307 110-119
GTAGT 10209545 5.113417 24.89442 80-89
ACAGT 7657170 4.9480543 34.45305 110-119
AGACG 9012580 4.9314528 96.43075 40-44
GTGAC 10368175 4.9132633 81.951225 45-49
ACTCC 8212905 4.859272 122.585396 30-34
GAGAC 8527295 4.6659174 134.80246 40-44
CCCCA 8263480 4.625944 98.36593 60-69
AAAAA 3715730 4.613834 27.17615 130-139
ACCAG 7483520 4.5754633 92.217735 45-49
CTCAG 8530235 4.5168123 33.878765 140-149
CACTC 7435630 4.3993874 99.40505 25-29
GCACA 7111290 4.34788 32.811417 110-119
GTGTC 10537675 4.3246856 23.733171 120-129
GGCCC 11741280 4.3137107 59.278423 60-69
CGTGT 10502115 4.3100915 23.207438 120-129
CTCCC 8714480 4.2249427 70.45078 30-34
TGACC 7867705 4.1659985 139.27411 45-49
TAGTA 6087805 4.1578035 30.730913 80-89
ATGCA 6209990 4.0128884 95.09433 25-29
AGTAG 6886325 3.98245 27.852142 70-79
GACCA 6452010 3.944792 103.54546 45-49
CCCAG 7807780 3.9116647 84.574394 60-69
TGAGG 8900305 3.7745855 67.335175 35-39
CAGGG 9314845 3.7376873 36.204445 50-59
CCACT 6317070 3.7375767 27.441425 240-247
ATGGT 7223450 3.6178412 25.177303 190-199
AGGAG 7351170 3.5998042 90.37142 35-39
GGGTT 9796580 3.598163 43.891922 50-59
TGCAC 6786620 3.5935574 116.847725 25-29
GACGG 8921600 3.5798926 104.378136 40-44
TACAC 4953670 3.576816 26.724693 110-119
ACGGT 7542025 3.5740094 93.084 40-44
CTGAG 7507315 3.5575607 103.17575 35-39
TGTCC 7639200 3.5031676 20.387852 120-129
AGATA 4310325 3.3991573 18.025026 150-159
TGGTG 9229130 3.3897455 17.953775 190-199
GCCCC 8248740 3.3863087 72.205956 60-69
CCTGA 6388480 3.3827403 84.00312 30-34
CCCTT 6601590 3.3827116 31.649557 20-24
GAGGA 6882570 3.3703349 118.70815 35-39
TCTTG 6939875 3.36358 18.118143 170-179
CTTCA 5350140 3.3456204 16.30537 200-209
CCAGG 7431780 3.3321424 41.06153 50-59
GCACT 6280995 3.3258264 90.31022 25-29
GGATG 7808355 3.3114936 70.68234 20-24
CCTGG 8303265 3.224197 30.185495 50-59
CTCTC 6289410 3.2227478 13.7662325 100-109
CCGTG 8242645 3.200658 23.44573 120-129
CTTGG 7774455 3.1906538 17.884275 20-24
TCAGG 6678535 3.164819 20.55884 140-149
GGTTG 8557925 3.14322 76.496895 15-19
TGTCT 6481010 3.1411798 19.560387 180-189
TTCCC 6106865 3.1292102 51.29472 50-59
CAGAT 4794710 3.0983362 16.85059 150-159
AGGCT 6514385 3.087032 20.966825 30-34
TCGCA 5815450 3.0793169 23.85054 100-109
CGGAT 6475400 3.068558 80.24419 20-24
GGTTC 7414100 3.0427628 49.472527 50-59
CGCAC 5994775 3.003357 24.478506 100-109
TCTCA 4778125 2.9879205 20.76194 130-139
GATGC 6274820 2.9735072 97.90251 25-29
AGGGT 6960445 2.951898 50.844803 50-59
GTTGG 8003360 2.9395354 54.572826 15-19
ACAGA 3932425 2.9341662 14.291604 140-149
TGGCC 7530955 2.924305 43.93972 60-69
CCAGT 5410380 2.8648303 37.80786 60-69
AGTCA 4420780 2.856703 32.332172 70-79
ACCAC 4147845 2.8337073 18.801405 230-239
GACTC 5345525 2.8304887 19.81992 190-199
TCTCT 5136325 2.7816725 14.775063 180-189
GCCGT 7152235 2.7772462 22.206768 120-129
TTCTT 4800695 2.74786 20.469059 170-179
CGGTG 7881660 2.7389681 67.00801 40-44
GCCCT 6272620 2.7216043 17.32004 20-24
TCAAA 3087865 2.720965 55.937107 70-79
GTTCC 5932660 2.7205863 55.913116 50-59
CGGCC 7277600 2.673768 12.554627 120-129
GATGG 6159465 2.6122057 17.641647 15-19
TCACA 3594045 2.5950937 11.548616 140-149
GCGGA 6383455 2.5614338 88.274025 20-24
AGGGA 5227665 2.5599422 9.352787 150-159
AGATG 4385105 2.5359623 16.18435 180-189
GGCGG 8552925 2.51677 43.519802 20-24
TTGGG 6674200 2.4513514 82.18869 15-19
TGTTC 5030550 2.438179 12.711266 140-149
CTGGC 6215475 2.413498 36.122524 60-69
TCCTC 4687205 2.4017642 16.505283 130-139
GGCTG 6911245 2.4017375 11.609525 140-149
TCCAT 3836540 2.3991158 15.107224 70-79
GTTCT 4905445 2.377544 18.00999 160-169
CACTA 3292300 2.3772175 14.719237 240-247
ACTCT 3784150 2.3663547 13.187613 200-209
ACAGC 3866855 2.3642154 10.879855 120-129
TGGAG 5547335 2.3526037 14.395051 25-29
CAGCT 4404615 2.3322713 12.001507 150-159
TACCA 3206120 2.314991 15.49777 230-239
GAGAT 4000460 2.3135173 15.77302 180-189
TCAGC 4330800 2.2931855 14.625976 140-149
CACGG 5026155 2.2535467 9.306892 120-129
TGTAG 4469695 2.238632 8.418494 210-219
AATAA 2077875 2.2344937 8.885204 110-119
CTGGA 4702030 2.2281942 13.972051 180-189
CTACT 3524125 2.2037523 7.5993032 180-189
CAGAG 4004210 2.1910014 9.706653 140-149
TCATT 3305660 2.184775 15.956132 150-159
TGGGG 7022070 2.1838918 54.280407 15-19
CATTT 3299800 2.1809022 17.082314 150-159
TCTGC 4736900 2.1722372 11.90685 210-219
TTCAC 3466870 2.167949 17.780312 200-209
TTCAT 3253225 2.1501198 15.505302 140-149
ACTAC 2946105 2.1272461 11.555137 230-239
TCTCG 4627820 2.1222157 17.402529 100-109
GAACT 3281210 2.120314 11.044305 160-169
GTGAA 3653615 2.1129324 17.059675 190-199
TTGTC 4352985 2.1097806 13.047639 170-179
TAGTC 3745275 2.0960026 27.1246 70-79
TTGGA 4174940 2.0910048 15.988306 170-179
GATAC 3220390 2.0810122 13.788376 150-159
CTCGC 4787930 2.0774171 16.496962 100-109
GATAT 3035835 2.073392 8.414794 180-189
ATCTC 3297105 2.0617893 9.247367 100-109
ATTTG 3468100 2.051336 15.075474 150-159
GTCTC 4444785 2.0382798 11.560833 180-189
ACACA 2431325 2.0270786 12.923318 110-119
AGCTC 3823185 2.0244002 12.577744 140-149
GCTGT 4891800 2.0076056 9.970908 140-149
ACACG 3280805 2.0059013 12.773095 110-119
CTCTG 4365470 2.0019078 14.160153 180-189
GTCAA 3090060 1.996793 40.674805 70-79
ATATA 2128685 1.9824994 9.60781 110-119
GTCCT 4311525 1.9771698 14.002156 130-139
GTTCA 3527525 1.9741414 14.580021 140-149
GAATC 3039675 1.9642344 13.602486 190-199
TACAG 3022655 1.9532362 13.247693 160-169
GGGGC 6596525 1.9410828 49.342022 15-19
CCTTC 3784475 1.9391979 13.622282 200-209
CAGGC 4307775 1.9314511 14.803857 140-149
TGAAT 2814660 1.9223355 13.219596 190-199
ATGTA 2804980 1.9157242 6.7617407 150-159
AACCA 2281265 1.9019685 14.7423725 70-79
ACGGC 4240910 1.9014711 13.992091 120-129
TTGCA 3389165 1.8967097 13.866088 150-159
GGGCG 6411105 1.8865215 61.334106 20-24
GAGTC 3972325 1.8824025 11.719754 210-219
AATAT 2017235 1.8787034 12.00387 110-119
CAGCC 3743585 1.8755202 10.107517 120-129
ATATG 2742445 1.8730145 8.642667 180-189
TTTGC 3845900 1.8640091 11.942498 150-159
AATCG 2876085 1.8585229 12.442454 190-199
TGGAA 3201545 1.8514946 9.009547 170-179
TCGGC 4758735 1.847839 7.740563 130-139
CTCCA 3116525 1.8439326 7.857417 150-159
TACTA 2396365 1.8287721 9.0468445 230-239
GTCAC 3435915 1.8193383 9.011737 130-139
CATAG 2811650 1.816885 7.937516 210-219
GTTGT 4157650 1.8034109 7.396764 210-219
CTGTT 3630300 1.7595136 12.510887 140-149
AGTAT 2571280 1.7561136 11.271303 220-229
AGAAC 2347155 1.7513219 11.234885 160-169
TTCAG 3121405 1.7468606 9.665202 150-159
GACGT 3668795 1.7385657 10.021699 170-179
GTCTG 4173295 1.7127296 8.874522 210-219
CGTAG 3611845 1.7115787 9.216923 210-219
CCAGA 2793440 1.7079239 23.4601 60-69
CACCA 2496225 1.7053605 12.747099 230-239
TGGTT 3927785 1.7037053 18.378387 10-14
AGTCT 3012595 1.6859664 12.497938 210-219
GGTCA 3552620 1.6835128 8.074142 130-139
ATTGT 2845945 1.6833395 7.361867 170-179
TGCAG 3529000 1.6723198 13.189502 150-159
AACTG 2572175 1.6621366 10.862844 160-169
TGACT 2966645 1.6602509 11.673224 190-199
GCAGA 3028825 1.6572955 13.793886 150-159
TCCCA 2784925 1.6477371 10.150034 240-247
GAGAA 2430590 1.6230526 10.297789 160-169
ATAGT 2374635 1.6218106 7.0817876 220-229
CTGGT 3942850 1.6181543 7.370592 160-169
ACTGA 2503130 1.6175197 9.014915 180-189
TTGTA 2727705 1.6134022 9.404757 210-219
GTGCT 3904710 1.6025017 8.478441 220-229
TAGTT 2708405 1.6019864 9.829853 220-229
CAAAA 1570915 1.5983629 19.189285 70-79
TGTGC 3867370 1.5871773 8.14326 220-229
GCTCC 3647815 1.5827372 6.1522384 150-159
TCTGG 3840750 1.5762523 11.661804 180-189
TGCTC 3414630 1.5658735 5.0638003 150-159
TCAGA 2420140 1.5638915 9.544177 130-139
CCTTG 3410100 1.5637965 20.366383 20-24
TTGGT 3603365 1.5629858 22.053389 25-29
CAAAG 2093520 1.5620732 28.542414 70-79
GGACG 3881845 1.5576345 7.4995484 170-179
ATAGA 1971785 1.5549657 9.621961 240-247
CTGTG 3779035 1.5509244 5.1553597 210-219
AATAG 1964710 1.5493865 8.925357 240-247
GCTCA 2912735 1.5423114 11.249811 140-149
GGCCG 4679515 1.5386258 13.132037 120-129
GAACC 2496850 1.526587 12.344902 70-79
CCCAC 2709265 1.5166621 10.165769 240-247
CCACC 2698225 1.5104818 9.572128 230-239
GTCCC 3438890 1.4920872 18.080702 50-59
GCTCT 3213380 1.4735849 9.869526 130-139
AAATA 1356940 1.4592187 5.0633945 9
CTTGA 2589905 1.4494125 8.641564 200-209
CGACT 2718095 1.4392483 9.08096 190-199
CTGCT 3136000 1.4381 5.944363 90-99
ACCAT 1988200 1.4355873 10.507681 45-49
CTCTT 2650705 1.4355386 8.3765745 200-209
GAGGG 3967990 1.4249333 8.6519785 200-209
CACTG 2683805 1.4210916 7.332983 230-239
TGATA 2077220 1.4186841 7.919577 180-189
CCTCG 3265035 1.4166542 9.350064 130-139
TGGAC 2976735 1.4106131 10.584706 170-179
GGGAG 3920225 1.4077805 6.8860745 150-159
TCGAC 2657270 1.407041 9.791288 190-199
TATAC 1831000 1.397317 6.402866 110-119
AGCTT 2486235 1.3913945 6.443836 150-159
AGATC 2144345 1.3856732 7.0003557 130-139
AATTG 2000640 1.3663821 8.952587 170-179
CATGT 2407670 1.3474265 6.6064153 70-79
CAGAC 2200230 1.3452321 13.341312 60-69
CTGAT 2398620 1.3423617 7.549723 180-189
GGAAT 2309835 1.3358072 8.518928 170-179
GCGTA 2809030 1.3311411 9.59739 210-219
CCATC 2249630 1.3310229 10.309237 240-247
GCTGA 2807685 1.3305038 18.10449 30-34
ATCGG 2790080 1.3221611 7.569961 190-199
TCCAC 2222620 1.315042 9.91776 240-247
CTCGA 2478330 1.3122911 8.733902 190-199
CCTCA 2191760 1.2967836 7.078882 130-139
TATGG 2574805 1.2895826 6.2756176 180-189
ACGTG 2708805 1.2836467 9.614764 170-179
ACGGA 2342635 1.2818298 8.792534 200-209
ACTTC 2042430 1.2771994 6.2201147 220-229
GAATT 1862400 1.271968 9.973512 170-179
AGAGC 2321405 1.2702134 7.537847 140-149
GTGTT 2906355 1.2606527 7.443155 160-169
ACTAT 1643785 1.254445 6.1797237 230-239
ACTCG 2357760 1.2484487 8.88919 190-199
AAAGT 1581305 1.2470301 21.295275 70-79
GGAGT 2925185 1.2405598 6.8995824 210-219
AGCCG 2765830 1.2400984 8.846139 120-129
GGCTC 3177235 1.2337351 8.190477 130-139
ACTGG 2599020 1.2316217 7.419524 160-169
TTGAG 2437995 1.2210617 8.276368 200-209
CCATG 2297135 1.2163475 6.7195144 70-79
GTGGA 2849765 1.2085747 18.806362 25-29
AAGTA 1531165 1.2074893 15.34418 70-79
ATAAC 1369350 1.2066438 7.3785906 80-89
ATAAA 1114825 1.1988544 5.391472 7
GATCT 2134265 1.1944184 5.2489753 130-139
TTCCA 1901205 1.1888866 7.592373 230-239
GGAGG 3307825 1.1878633 13.989383 30-34
GAAGA 1763135 1.1773523 17.096983 35-39
ATATC 1528335 1.16634 6.810697 70-79
GCATA 1799230 1.1626604 7.6701264 210-219
GTGGT 3144590 1.1549691 9.381271 50-59
ACCGT 2173725 1.1510011 15.969046 45-49
TTGGC 2783485 1.1423484 7.420633 60-69
CTGAA 1767535 1.142179 13.334563 35-39
ATCCA 1576805 1.138538 15.018749 240-247
GTCCA 2146790 1.1367387 14.2531395 70-79
ACTGT 2030315 1.1362439 5.529136 230-239
TGGTC 2767740 1.1358867 10.644952 50-59
GCTTC 2475410 1.1351682 5.7644997 150-159
TCTAC 1814285 1.1345327 7.3314357 180-189
AAAAG 1244435 1.133161 12.610924 70-79
GTCTA 1997090 1.1176499 7.4201508 180-189
ATACC 1547750 1.1175587 6.4098363 70-79
TATCA 1461790 1.1155566 6.350073 70-79
TACTG 1991520 1.1145327 6.752037 180-189
TCACG 2099280 1.1115818 9.038439 200-209
TGCCC 2556210 1.1091045 6.010718 60-69
AGGTT 2203275 1.103503 23.076847 10-14
TCTGT 2273440 1.1018783 6.244851 210-219
CTTCT 2022500 1.0953225 5.5081525 210-219
GAGCT 2307230 1.0933485 6.6302495 140-149
AGTTC 1930280 1.0802603 5.170185 160-169
CAATA 1224920 1.079375 6.1384287 100-109
CGGCT 2765920 1.0740197 7.6602473 130-139
AGGGG 2974345 1.0681083 5.017536 60-69
GGGGT 3433880 1.0679502 8.023175 10-14
TATGT 1804180 1.067149 7.301415 220-229
CTCGG 2727715 1.0591844 8.255691 130-139
CTGCG 2724380 1.0578895 6.6285286 210-219
TAGAT 1540985 1.0524505 7.6537695 240-247
CTACC 1776545 1.051116 8.370081 230-239
GCGGT 3012650 1.0469309 6.0993423 130-139
CCATA 1436030 1.0368909 8.81319 70-79
GTCCG 2668155 1.0360571 5.118483 120-129
CTGCC 2383480 1.0341593 5.6868896 200-209
AAGAG 1543735 1.0308456 12.676535 35-39
AGAGA 1529980 1.0216606 11.587425 35-39
ACCCA 1484985 1.0145059 12.657742 240-247
CATGG 2133460 1.0110025 5.1479106 190-199
CAGCG 2217300 0.9941574 6.2523003 160-169
TGCGT 2385025 0.9788195 7.8592505 210-219
TGAAC 1513310 0.9778992 6.261523 190-199
TTCTG 2001645 0.9701462 5.543664 210-219
ATGGG 2275265 0.96493125 27.383463 15-19
AGCTG 2035520 0.9645906 5.1028905 80-89
TATAA 1034045 0.96303284 6.2107306 240-247
AACTT 1254260 0.9571812 5.461601 200-209
CGAAC 1549295 0.9472471 13.018349 70-79
CGGAG 2328490 0.9343331 6.445041 210-219
TGTAA 1365955 0.9329098 6.6261954 230-239
GTATG 1859310 0.9312293 5.1768 220-229
CCCAA 1344835 0.91875875 10.018998 240-247
ACTAA 1038265 0.9148984 11.450519 240-247
ACAAT 1032610 0.9099153 7.0092382 100-109
CCAGC 1808780 0.9061911 5.8075833 80-89
TAGGC 1899475 0.90012187 5.539846 160-169
ACGTC 1697765 0.8989772 9.646225 70-79
CCATT 1425080 0.8911499 5.401966 50-59
TGCAT 1587485 0.8884188 5.929114 210-219
CCAAT 1217795 0.87931347 10.664396 240-247
CTTCC 1713845 0.87818897 5.4395804 230-239
TCGAA 1354585 0.87533116 16.343987 70-79
CGGTC 2244390 0.8715071 5.910553 130-139
CGTGG 2494290 0.86679476 6.4749336 50-59
CATCA 1190435 0.85955805 5.440738 230-239
AGCGT 1806300 0.85596806 6.3708687 160-169
AAAGA 936655 0.8529017 5.794144 8
TGAAG 1464520 0.8469507 8.75373 35-39
GGTGG 2626320 0.81679595 19.248592 25-29
ATCAA 907645 0.79979867 11.446828 70-79
GAGGC 1985375 0.79665416 11.837445 30-34
GGTGC 2271985 0.789541 5.0775113 220-229
GACCG 1746940 0.7832648 14.85708 45-49
TAACC 1065615 0.7694313 5.9844427 80-89
TGAGA 1326565 0.76716954 5.261726 240-247
GAAAA 842315 0.7669973 5.22801 8
AATCC 1056555 0.7628895 9.58885 240-247
AACCG 1240635 0.75853074 5.3494725 190-199
GGGCC 2302665 0.757117 14.522645 15-19
GGAAG 1536365 0.7523446 6.222204 30-34
AAAGG 1126430 0.75218576 8.602199 10-14
ATGAT 1081500 0.7386348 6.7833066 240-247
AGCCA 1200535 0.73401326 5.7474566 80-89
GTCGA 1532135 0.7260469 12.030357 70-79
CTAAT 949025 0.7242428 8.838496 240-247
AAGGT 1243340 0.7190395 10.021988 10-14
CGTCC 1640760 0.7119034 8.040153 70-79
TGGGC 2025285 0.70381004 15.829664 15-19
GTAGC 1457310 0.690589 5.3463736 80-89
GGTCC 1773410 0.68862337 10.85593 50-59
CATAC 933280 0.6738783 7.576161 70-79
CAATC 911665 0.65827113 9.326122 240-247
TATGA 955025 0.65225583 5.6331267 240-247
AAAGC 864885 0.64533114 8.68159 70-79
TAGGT 1275995 0.6390779 5.2951612 10-14
GAGGT 1457280 0.6180269 6.742871 10-14
AAGAA 678580 0.6179031 5.0140486 9
GGGTC 1777270 0.61762184 5.9404287 70-79
AGGAT 1067905 0.61758316 5.1258535 10-14
TAAAA 572390 0.6155336 5.8415465 6
AGAAG 921530 0.6153615 5.286984 9
CATCC 968685 0.5731351 6.9615135 240-247
AATGG 984630 0.56942415 6.4521756 10-14
CGATG 1175900 0.5572347 12.029784 15-19
GAAGG 1123000 0.54992336 5.613677 10-14
AAGGG 1060905 0.519516 6.3461437 10-14
GGTCG 1459510 0.5071966 6.0451336 70-79
AAGCA 679240 0.50681275 5.768593 70-79
TGATC 900580 0.5039998 5.279215 240-247
GATCC 913705 0.48381245 5.2886643 240-247
ATAAG 600145 0.4732793 5.259007 9
CGGTT 1121070 0.46008962 8.436702 10-14
ACGAT 671020 0.43361235 5.4678392 10-14
TAAGG 744755 0.43070138 5.753728 10-14
TAAAG 540755 0.42644385 5.405118 9
TGGGT 1101015 0.40438923 5.212787 10-14
AAGCT 615290 0.3975997 7.67676 30-34
Produced by FastQC (version 0.10.1)