FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_3_SL248349.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_3_SL248349.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9960256
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11563045 8.819645 62.72106 110-119
ATACA 10551285 7.629849 38.659176 110-119
CACAG 15408790 7.5569577 42.93314 110-119
TCCCT 18119630 7.100035 108.27727 30-34
CAGTA 13077085 6.7648425 39.948357 110-119
AGTAA 10021300 6.4728174 49.003044 110-119
GGAGA 15991400 6.257232 96.121666 35-39
CCCTG 18074795 5.997564 71.06206 30-34
GTAAT 10972115 5.9869466 40.5561 110-119
GGTGA 17743160 5.86506 102.19028 45-49
AATAC 7655970 5.5361876 40.6664 110-119
GTAGT 13708465 5.351062 28.66152 80-89
AGACG 12070390 5.287598 103.58042 40-44
ACAGT 10147260 5.249229 36.17798 110-119
GTGAC 13656060 5.053686 85.39449 45-49
CCCCA 10883060 4.7857304 102.53074 60-69
GAGAC 10915965 4.7818856 143.4888 40-44
ACTCC 10197115 4.7298 123.340805 30-34
CTCAG 11335520 4.6964054 35.09937 140-149
TAGTA 8431915 4.600883 36.627274 80-89
ACCAG 9352385 4.586705 95.734955 45-49
CACTC 9501215 4.407016 100.976875 25-29
GCACA 8966240 4.397327 34.489525 110-119
GGCCC 15618270 4.388589 59.405033 60-69
CGTGT 13819000 4.3202124 23.55424 120-129
GTGTC 13812520 4.318187 23.917505 120-129
TGACC 10371775 4.297118 145.56401 45-49
AGTAG 9243840 4.2712774 29.718262 80-89
CTCCC 11365995 4.222312 70.80312 30-34
ATGCA 7914100 4.094004 98.73515 25-29
CCCAG 10219335 4.014011 87.66463 60-69
GACCA 8177005 4.0102615 108.233986 45-49
TGAGG 11570810 3.82477 70.18712 35-39
CCACT 8213690 3.8098137 28.334072 240-247
TACAC 6501990 3.7656012 28.238548 110-119
AGGAG 9620030 3.7641954 96.6296 35-39
ATGGT 9516525 3.7147489 25.671352 190-199
CAGGG 11778820 3.6912632 36.182133 50-59
ACGGT 9939615 3.6783447 97.69587 40-44
GACGG 11716080 3.6716018 109.38115 40-44
AGATA 5670435 3.662568 19.93339 150-159
CTGAG 9870240 3.6526706 106.4191 35-39
TGCAC 8653660 3.5852873 118.110565 25-29
CCCTT 9100540 3.5659752 34.413975 20-24
GAGGA 9072445 3.5499327 126.510345 35-39
TGTCC 10094735 3.533172 20.66941 120-129
GGGTT 12398390 3.4622023 42.580917 50-59
GCCCC 10927315 3.437532 73.409004 60-69
CCTGA 8277315 3.4293642 86.52657 30-34
TGGTG 12206570 3.408638 17.844095 190-199
GGATG 10148315 3.3545592 71.44233 20-24
TCTTG 9032330 3.334555 18.141195 170-179
GCACT 8029225 3.326578 91.46243 25-29
CCAGG 9453145 3.3165824 41.068787 50-59
CCGTG 11156525 3.3066573 23.700468 120-129
AGGCT 8851525 3.2756758 24.212162 30-34
CTTGG 10441850 3.2644196 19.61623 20-24
CTCTC 8327275 3.2629774 13.71443 130-139
CTTCA 6659535 3.2581995 17.077255 200-209
CAGAT 6285300 3.2514179 18.265202 150-159
TCAGG 8751565 3.2386837 21.088453 140-149
TCGCA 7666160 3.1761575 24.622314 100-109
CCTGG 10675630 3.164126 29.808264 50-59
TGTCT 8512900 3.1427922 19.622795 180-189
TCTCA 6389840 3.1262503 22.133512 130-139
CGCAC 7884920 3.097085 25.19948 100-109
CGGAT 8297140 3.0705152 81.21284 20-24
GGTTG 10965980 3.0622077 74.52658 15-19
ACAGA 4993180 3.0575829 14.734581 140-149
TTCCC 7753030 3.037964 50.40858 50-59
ACCAC 5454535 2.9948668 20.416803 230-239
GATGC 8009690 2.9641387 97.99618 25-29
GGTTC 9376765 2.9314435 48.110226 50-59
TGGCC 9843715 2.9175568 43.7353 60-69
AGGGT 8765725 2.8975394 50.644936 50-59
GACTC 6959895 2.8835456 19.873837 190-199
GTTGG 10215520 2.8526447 53.693264 15-19
GCCCT 8551595 2.8375838 18.547157 20-24
TCAAA 3909790 2.8272488 58.843224 70-79
TCTCT 6797120 2.8093405 14.966976 180-189
CGGTG 10372165 2.7459228 68.64441 40-44
CGGCC 9758290 2.741989 13.027018 120-129
TTCTT 6286615 2.7407196 20.399893 170-179
GCCGT 9204270 2.7280328 22.383038 120-129
AGTCA 5267915 2.725119 32.460064 70-79
CCAGT 6559695 2.7177389 36.734642 60-69
GATGG 8211340 2.7142856 19.219194 15-19
TCACA 4607895 2.6686437 11.863353 140-149
GTTCC 7623490 2.6682327 54.5591 50-59
AGATG 5721880 2.6438947 17.194382 180-189
TCCAT 5268325 2.5775454 17.883333 70-79
AGGGA 6584830 2.5765605 9.341612 150-159
GCGGA 8150805 2.5543106 89.147156 20-24
TGGAG 7504980 2.4807963 16.494097 25-29
TGTTC 6700105 2.4735446 13.057523 140-149
TCCTC 6307210 2.4714308 16.94263 130-139
GGCTG 9331935 2.470533 12.51644 30-34
ACAGC 5029955 2.4668486 11.255259 120-129
TACCA 4239660 2.4553819 16.885645 230-239
GGCGG 10902505 2.4441936 43.05635 20-24
ACTCT 4991330 2.4420247 13.635198 200-209
GAGAT 5185475 2.3960392 16.862053 180-189
TTGGG 8513110 2.377253 80.51266 15-19
GTTCT 6424840 2.3719223 17.913054 160-169
CAGCT 5699380 2.361303 12.348978 150-159
CACGG 6698600 2.3501663 9.842372 120-129
CTGGC 7893760 2.3396137 35.182503 60-69
CTGGA 6304880 2.3332412 14.739662 180-189
TGTAG 5950705 2.3228414 8.2067375 210-219
AATAA 2553590 2.3056023 9.708634 110-119
ACTAC 3933315 2.2779636 13.103853 230-239
CATTT 4392395 2.266749 17.78316 150-159
CTACT 4614310 2.2575662 7.72281 230-239
TCATT 4348090 2.2438848 16.330578 150-159
CACTA 3867005 2.2395604 15.646653 240-247
CAGAG 5109860 2.2384434 9.736298 140-149
GTGAA 4804865 2.220172 18.150536 190-199
TTCAT 4293595 2.2157621 16.194954 140-149
TTCAC 4480820 2.192256 18.667723 200-209
TCAGC 5285675 2.1899016 14.732939 140-149
GATAC 4210705 2.1782188 14.863422 150-159
TTGTC 5793285 2.1387649 13.330322 170-179
ATTTG 4608160 2.1241648 15.672171 150-159
TGGGG 8952095 2.1169095 53.2696 15-19
TAGTC 4803170 2.0990384 26.822487 70-79
ATATA 2746585 2.0949416 11.069825 110-119
CTCGC 6287265 2.0862355 16.57245 100-109
TTGGA 5338900 2.084025 16.004904 170-179
ATGTA 3806055 2.076778 7.9303346 70-79
TCTGC 5918860 2.0716097 12.091827 210-219
CTCTG 5875155 2.0563126 14.606433 180-189
AGCTC 4960090 2.055009 12.676388 140-149
ACACA 2994985 2.0532272 14.199264 110-119
TACAG 3968300 2.0528216 13.957031 160-169
GAACT 3943410 2.039946 11.063436 160-169
GAATC 3936700 2.036475 14.418314 190-199
GCTGT 6510235 2.0352845 10.228892 140-149
GTCTC 5807790 2.0327349 11.857026 180-189
GTCAA 3910230 2.0227818 41.407944 70-79
GTCCT 5772135 2.0202556 14.144129 130-139
TCTCG 5756235 2.0146904 15.949358 100-109
CAGGC 5736030 2.012454 15.389589 140-149
GATAT 3673780 2.004602 8.600132 180-189
TGAAT 3657285 1.9956015 13.847229 190-199
GTTCA 4562140 1.9937056 15.245081 140-149
ATCTC 4068795 1.9906715 7.7251024 100-109
AATCG 3840735 1.9868317 13.471249 190-199
AATAT 2603885 1.9860983 13.718197 110-119
ACGGC 5654460 1.9838357 14.8202305 120-129
AACCA 2884685 1.9776105 15.418024 70-79
TTGCA 4501880 1.9673713 14.562645 150-159
ACACG 3996430 1.9599756 13.653851 120-129
TACTA 3204330 1.957463 10.37392 230-239
TGGAA 4227035 1.9531755 9.551067 170-179
ATATG 3568460 1.9471338 8.788467 180-189
CCTTC 4963040 1.9447284 14.078827 200-209
TTTGC 5240495 1.9346856 12.260071 150-159
GAGTC 5201455 1.9248977 12.335203 210-219
CAGCC 4845235 1.90314 10.202986 120-129
GGGGC 8419805 1.8876057 48.711567 15-19
TCGGC 6368335 1.8874964 8.060725 130-139
CATAG 3641975 1.8840122 8.455802 210-219
AGTAT 3392295 1.8510092 12.162886 220-229
GGGCG 8191215 1.836359 59.966698 20-24
GACGT 4930455 1.8246092 9.935994 170-179
CCAGA 3663600 1.796745 27.217821 60-69
GTTGT 5443135 1.7949232 7.138446 210-219
AGAAC 2930365 1.7944144 11.737685 160-169
CGTAG 4814225 1.7815958 9.916522 210-219
CTGTT 4806530 1.7744746 12.790808 140-149
GCAGA 3993525 1.7494177 14.881144 150-159
ATTGT 3747135 1.7272693 7.757124 170-179
AGTCT 3949985 1.7261871 13.149948 210-219
CACCA 3136150 1.7219344 13.620424 230-239
CTCCA 3684315 1.7089218 7.858634 150-159
GGTCA 4590455 1.6987855 7.8837404 130-139
TGCAG 4589665 1.6984931 13.651529 150-159
TGACT 3883650 1.6971982 11.898977 190-199
GTCAC 4082590 1.6914529 8.848762 130-139
ATAGT 3089535 1.6858078 7.286653 220-229
TCCCA 3628885 1.6832113 10.844801 240-247
AACTG 3240565 1.6763607 11.025487 160-169
GAGAA 3043875 1.6648936 10.7418995 160-169
TGGTT 5024200 1.6567758 18.370129 10-14
CCTTG 4710725 1.6487604 22.346262 20-24
AATAG 2536165 1.6381241 10.01254 240-247
TCAGA 3154660 1.6319216 10.2727375 130-139
TAGTT 3530225 1.627283 10.068036 220-229
CAAAA 1897770 1.6244581 21.553642 70-79
ACTGA 3136280 1.6224136 9.075513 180-189
TTGTA 3513755 1.6196911 9.239907 210-219
TGTGC 5162700 1.6140069 8.235052 220-229
GCTCC 4846745 1.6082432 6.3322654 150-159
GTGCT 5129655 1.6036762 8.380137 220-229
ATAGA 2477245 1.6000671 10.361689 240-247
GAACC 3257630 1.5976447 12.597079 70-79
CAAAG 2602875 1.5938753 29.735485 70-79
TGCTC 4553395 1.5936949 5.2995763 150-159
GTCTG 5081235 1.5885388 9.070081 210-219
CTGTG 5073005 1.5859658 5.299126 210-219
TTCAG 3628410 1.5856553 9.428527 150-159
TCTGG 5069890 1.5849919 11.950869 180-189
GCTCA 3797320 1.573263 11.474763 140-149
GTCCC 4737495 1.5719919 20.177412 50-59
TTGGT 4765965 1.5716202 23.342712 25-29
CTGGT 5023480 1.5704827 7.0974145 160-169
GGCCG 6224560 1.5622814 13.309203 120-129
GGACG 4958485 1.5538969 7.415008 170-179
AAAAA 1452975 1.5529078 7.92327 8
CCCAC 3490965 1.5351212 10.537938 240-247
CCACC 3476365 1.5287011 9.991906 230-239
ACCAT 2615560 1.514791 11.750885 45-49
GCTCT 4210485 1.4736757 10.200288 130-139
CCTCG 4418695 1.4662079 9.686456 130-139
TATAC 2389160 1.4594914 7.129926 110-119
CGACT 3497005 1.4488397 8.891028 190-199
CAGAC 2937740 1.4407605 16.230753 60-69
TGATA 2637515 1.439163 8.066524 180-189
CATGT 3275295 1.4313402 7.83167 70-79
CACTG 3447860 1.4284786 7.43673 230-239
GCTGA 3840895 1.4213965 20.82861 30-34
TCGAC 3422050 1.4177853 9.537994 190-199
AATTG 2596465 1.4167638 9.603324 170-179
AAATA 1566175 1.4140785 5.230882 9
TGGAC 3801950 1.4069843 10.510959 170-179
GGGAG 4983525 1.3949816 6.687976 150-159
CTCTT 3373665 1.3943808 8.239779 200-209
GAGGG 4952205 1.3862146 8.482495 200-209
GCGTA 3700500 1.3694407 10.364613 210-219
CCATC 2930415 1.359235 10.851318 240-247
CCTCA 2913140 1.3512223 7.342568 130-139
CTGAT 3078415 1.3453014 7.566091 180-189
ACGGA 3070605 1.3451202 9.4899645 200-209
CTCGA 3221245 1.3345901 8.570182 190-199
ACTTC 2719570 1.3305587 6.680876 220-229
TATGG 3398540 1.3266106 6.333553 180-189
TCCAC 2850440 1.3221397 9.967352 240-247
CTGCT 3772425 1.3203541 5.7782564 90-99
GAATT 2419495 1.3202001 10.60115 170-179
GTAGG 3967450 1.3114538 5.1061125 160-169
GTGGA 3964715 1.3105496 21.054295 25-29
CTTGA 2994040 1.3084286 8.532612 200-209
AGATC 2518785 1.3029803 7.180561 130-139
ACCGT 3142745 1.3020668 19.07818 45-49
CCATG 3136305 1.2993985 7.95442 70-79
ACGTG 3469995 1.2841378 9.55937 170-179
GTGTT 3858615 1.2724135 7.616352 160-169
AGCCG 3622015 1.2707638 9.11239 120-129
ACTAT 2078570 1.2697582 6.254468 230-239
GGAGT 3818510 1.2622211 7.069793 210-219
GGCTC 4227160 1.2528784 8.494863 130-139
GGAGG 4474675 1.2525451 15.676203 30-34
GTCCA 3013350 1.2484573 16.6982 70-79
AAAGT 1932175 1.2480035 21.850464 70-79
ACTCG 3011250 1.2475874 8.777088 190-199
ATAAC 1718140 1.2424219 7.8150506 80-89
ATCGG 3345085 1.2379125 7.935526 190-199
AAGTA 1914590 1.2366452 16.098083 70-79
GGAAT 2671580 1.2344501 8.8815565 170-179
ACTGG 3306790 1.2237409 7.3711076 160-169
GATCT 2792995 1.2205697 5.6647153 130-139
GTGGT 4370480 1.2204398 10.771556 50-59
TTGAG 3090800 1.2064853 8.213073 200-209
TTGGC 3850380 1.2037383 8.133909 60-69
AGCTT 2750380 1.2019465 6.3458424 150-159
TGGTC 3832420 1.1981236 12.244624 50-59
GCATA 2303055 1.1913818 7.964672 210-219
TTCCA 2428660 1.1882299 7.5152087 230-239
ATACC 2047280 1.1856738 7.6853447 70-79
TGCCC 3550615 1.1781623 6.862487 60-69
ATAAA 1301975 1.1755357 5.6924844 7
ACTGT 2648380 1.1573714 5.582275 230-239
AGAGC 2629065 1.1516975 7.297719 140-149
TCACG 2767935 1.1467798 9.579087 200-209
ATCCA 1963185 1.1369706 15.916971 240-247
TCTAC 2317355 1.1337737 7.40908 180-189
CCATA 1949710 1.1291666 10.621677 70-79
TATCA 1841500 1.1249366 6.743433 70-79
GTCTA 2562545 1.1198605 7.479474 180-189
TCTGT 3021060 1.1153148 6.3878937 210-219
AGGTT 2848255 1.1118084 23.5863 10-14
AGTTC 2543565 1.111566 5.450527 160-169
CAATA 1522995 1.1013087 6.6379776 100-109
GCTTC 3145555 1.1009488 5.632315 150-159
AAAAG 1434220 1.0965776 14.29293 70-79
TACTG 2507850 1.0959582 6.635319 180-189
TATGT 2376190 1.0953221 7.7701273 220-229
GAGCT 2947875 1.0909175 6.5143905 140-149
CTACC 2351935 1.0909147 8.951999 230-239
CGGCT 3663080 1.085692 7.9348145 130-139
CTCGG 3640325 1.0789477 8.410092 130-139
CTGCG 3556300 1.0540438 6.972818 210-219
CTGCC 3140495 1.0420767 5.747842 200-209
GAAGA 1900585 1.0395538 17.542011 35-39
TAGAT 1904415 1.0391461 7.942662 240-247
AGAGA 1899250 1.0388236 13.136719 35-39
ATATC 1695020 1.0354548 7.2869716 70-79
AACTT 1694315 1.0350242 6.0147357 200-209
ATACG 1989795 1.029331 5.46207 120-129
GGGGT 4338655 1.0259652 7.8500066 10-14
ATGGG 3095625 1.023269 30.098795 15-19
CATGG 2761305 1.0218737 5.330781 190-199
GCGGT 3852730 1.0199702 5.8837233 130-139
CTGAA 1960500 1.0141766 14.350539 35-39
AGAAA 1320520 1.0096447 5.159562 7
TGCGT 3220605 1.0068527 8.311997 210-219
ACCCA 1814625 0.99633795 13.224365 240-247
TGCTA 2258735 0.9870922 5.0815744 220-229
TATAA 1292020 0.98548067 6.4832687 240-247
CTTCT 2381650 0.9843678 5.422893 210-219
ACTAA 1353675 0.9788701 12.945985 240-247
AAAAT 1081925 0.97685564 5.022877 8
TAGGC 2639105 0.9766512 5.8150516 160-169
CGAAC 1986870 0.9744237 13.230221 70-79
CAGCG 2759075 0.9680061 6.4265733 160-169
TGTAA 1772185 0.9669947 6.966916 230-239
TCCCC 2602940 0.9669566 5.157438 80-89
AGCTG 2609225 0.9655935 5.1316447 90-99
CGGAG 3060475 0.9590959 6.5929265 210-219
CCCAA 1719840 0.9442953 10.132692 240-247
CGTGG 3525680 0.93338716 7.4802313 50-59
ACAAT 1280655 0.9260678 7.5349255 100-109
CCATT 1889705 0.92454433 5.6437893 50-59
GAGTT 2355560 0.9194862 5.222403 160-169
AAGAG 1655085 0.9052739 13.055935 35-39
TGCAT 2066515 0.90308994 6.0954514 210-219
TCGAA 1731730 0.8958327 16.736979 70-79
CCAAT 1528170 0.8850335 10.928638 240-247
TGAAG 1897855 0.8769371 9.531022 35-39
ACGTC 2086045 0.86426675 11.443808 70-79
TTCTG 2339220 0.8635931 5.379115 210-219
CATCA 1490080 0.86297387 5.597342 230-239
TGAAC 1657365 0.8573634 6.775552 190-199
GGTGG 3614360 0.8546907 20.794611 25-29
CGGTC 2869600 0.85051423 5.7206826 130-139
CCAGC 2164260 0.8500907 5.7912335 80-89
CTTCC 2147375 0.8414321 5.3210893 230-239
ATCAA 1155030 0.8352257 12.801774 70-79
GAGGC 2660000 0.8335945 13.416524 30-34
GTATG 2135295 0.8335065 5.4112234 220-229
AGCGT 2236305 0.8275874 6.6072474 160-169
AACCG 1652235 0.8103083 5.987082 190-199
TAACC 1381520 0.80010164 6.218009 80-89
CGTCC 2371105 0.7867783 9.351446 70-79
GGGCC 3127000 0.7848352 15.911127 15-19
CTAAT 1267500 0.77429116 9.940819 240-247
AAAGA 1004655 0.7681403 5.895735 8
AAAGG 1400700 0.76613414 9.106837 10-14
AATCC 1309840 0.75858855 9.852908 240-247
TGAGA 1635350 0.7556421 5.679455 240-247
GACCG 2149315 0.75407517 17.565329 45-49
GGTCC 2534020 0.75105244 12.427516 50-59
ATGAT 1366290 0.74551755 6.818443 240-247
AGCCA 1517455 0.74420786 5.9665065 80-89
TGGGC 2765460 0.7321268 17.260174 15-19
CATAC 1244720 0.7208746 9.189487 70-79
GTAGC 1936200 0.7165279 5.625347 80-89
GTCGA 1930095 0.7142685 11.832705 70-79
AAGGT 1538545 0.7109116 10.108169 10-14
AAAGC 1094900 0.67046404 9.689755 70-79
CAATC 1142365 0.66159606 9.439247 240-247
TAGGT 1684480 0.65753204 5.5469446 10-14
CATTG 1504405 0.6574416 5.1310573 50-59
TATGA 1201420 0.6555561 5.6333117 240-247
GGAAG 1633625 0.63921666 5.881086 30-34
AGGAT 1374670 0.63519025 5.823621 10-14
GAGGT 1917330 0.63377976 6.9811835 10-14
ATAGG 1338920 0.61867136 5.2124877 10-14
CCGTA 1487725 0.61637753 5.3113756 80-89
AGAAG 1124860 0.6152593 5.6088133 9
AATGG 1282550 0.59262466 6.820834 10-14
CGATG 1600225 0.5921937 13.1363325 15-19
CATCC 1274410 0.5911187 7.478808 240-247
GGGTC 2109940 0.5585846 5.6657257 70-79
TAAAA 617690 0.557704 6.104624 8
AAATG 859790 0.5553435 5.201873 9
GAAGG 1379940 0.53995305 5.6052423 10-14
AAGAA 702590 0.5371871 5.3999376 8
AAGGG 1335965 0.52274615 6.3943253 10-14
AAGCA 841695 0.5154135 6.506798 70-79
GAAAA 668655 0.511241 6.316926 8
TGATC 1159335 0.5066422 5.309669 240-247
GATCC 1181715 0.4895949 5.394306 240-247
GGTCG 1846540 0.48885226 5.774007 70-79
ATAAG 712170 0.45999482 5.5767965 9
ACGAT 873605 0.45192033 6.1196375 10-14
CGGTT 1399655 0.43757197 8.153161 10-14
TAAGG 931120 0.43024024 5.925647 10-14
TAAAG 628325 0.40583882 5.6302433 9
AAGCT 772835 0.39979148 8.004995 30-34
GAAAG 705255 0.38574994 5.098123 9
TGGGT 1375500 0.38410312 5.081199 10-14
Produced by FastQC (version 0.10.1)