FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_4_SL248350.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_4_SL248350.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12270222
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 59479 0.48474265583784876 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 49464 0.40312229069694094 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 45053 0.36717347086303737 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 42209 0.34399540611408663 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 42043 0.34264253735588485 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 41917 0.34161566106953895 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39741 0.3238816706005808 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 39017 0.31798120685999 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38121 0.310678975490419 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35742 0.29129057322679247 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 35206 0.2869222741039241 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34865 0.28414318828135304 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32304 0.2632715202707824 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31941 0.26031313858869054 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31411 0.2559937383366006 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31256 0.25473051750815917 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30053 0.24492629391709456 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29648 0.24162562013955413 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29202 0.23799080407836143 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29162 0.2376648116065056 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28769 0.2344619355705219 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28747 0.23428263971100116 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28489 0.23217998826753095 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27635 0.2252200489934086 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26913 0.21933588487641054 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26869 0.2189772931573691 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26553 0.21640195262970793 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26220 0.21368806530150797 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26141 0.2130442301695927 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25570 0.20839068763385046 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25238 0.20568495011744695 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24632 0.20074616416883087 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 24370 0.19861091347817506 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24246 0.19760033681542194 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23678 0.19297124371506888 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 23594 0.1922866595241716 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 23265 0.18960537144315726 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23244 0.18943422539543295 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22730 0.1852452221320853 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22524 0.1835663609020277 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21257 0.17324054935599373 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21149 0.17236036968198293 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21006 0.1711949465950983 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20784 0.16938568837629833 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20676 0.16850550870228753 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20569 0.16763347884007315 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 20381 0.16610131422235067 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20013 0.16310218348127686 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19448 0.15849753981631304 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19300 0.1572913676704464 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19098 0.15564510568757436 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18936 0.15432483617655818 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18401 0.14996468686548622 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18216 0.14845697168315292 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17987 0.1465906647817782 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17970 0.14645211798123947 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 17605 0.14347743667555485 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17265 0.14070650066478016 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17178 0.1399974670384937 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17083 0.13922323491783606 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17059 0.13902763943472254 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16967 0.1382778567494541 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16327 0.13306197719976054 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16216 0.13215734809036056 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14271 0.11630596414636996 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14061 0.11459450366912677 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14053 0.1145293051747556 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13346 0.1087673882347035 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 13125 0.10696627982769995 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13090 0.10668103641482607 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12542 0.10221493955040098 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 25151760 7.7426867 65.91836 35-39
TATTA 12344725 7.4056344 127.61387 240-247
TGGAT 19056840 7.30935 79.510735 35-39
TCACC 19390610 6.437532 74.45458 160-169
TCTCC 17004615 6.0430436 84.93949 20-24
CTGTG 17987740 5.9273543 85.642044 25-29
TCCCT 16503355 5.864907 73.00492 15-19
AGTGG 19655910 5.826586 40.06845 100-109
CTGAG 18205985 5.604508 49.40682 15-19
TGTAT 11243150 5.5798545 59.70149 240-247
TTACT 10549895 5.4373255 113.995026 240-247
ATTAC 10565820 5.0872073 106.68423 240-247
TGGGT 15482775 4.9128184 45.37134 100-109
CCTGA 15047990 4.8106537 50.704067 15-19
CTCTG 13824800 4.730919 73.39247 35-39
ACACG 15547650 4.6433277 48.87162 230-239
TTCAC 11210510 4.6372223 74.96457 40-44
GACAC 15482820 4.623966 45.82991 230-239
CTCCA 13689195 4.5447063 40.386364 80-89
TGAGA 12263200 4.3941083 93.034874 15-19
TACTG 11008830 4.385018 80.28543 240-247
AAGGG 15584135 4.315615 39.72141 90-99
ATTCA 8911670 4.290771 111.88128 40-44
TCTGG 13018025 4.2897243 135.73178 35-39
TGGAG 14303155 4.2398725 39.43043 90-99
CCCTG 15270975 4.194196 84.92445 10-14
GAAGG 15015685 4.1581984 31.38563 90-99
TCAGT 10304200 4.104351 97.11883 7
AGCCT 12811705 4.0957417 50.517235 30-34
CAGCC 15952300 4.093024 54.641003 30-34
CCAGG 16534900 4.0852647 53.05896 80-89
ACCAT 10570840 4.0848966 68.33991 160-169
ACTAC 10468125 4.045204 36.568466 130-139
GAGAC 13907240 3.9994776 91.2409 8
CACCA 12879090 3.9944046 57.787544 160-169
GTATT 8035470 3.9879172 69.44211 240-247
TACTA 8274005 3.9837492 28.959818 130-139
TCCAG 12161800 3.8879752 39.866543 80-89
GGGTC 15110240 3.8481205 121.217125 8
ACTGT 9601165 3.8243196 69.35372 240-247
CACCT 11485730 3.813173 44.666573 20-24
TGAAG 10491225 3.7591798 39.207348 150-159
CTGGG 14624775 3.724487 129.10086 3
GACTC 11515215 3.6812696 50.92454 20-24
CAAGA 10578300 3.6772583 45.07618 180-189
GATTC 9215435 3.6706762 58.99248 40-44
AGACT 9804765 3.6484334 68.23756 15-19
GGACA 12562335 3.6127067 28.242184 220-229
ACTGG 11688370 3.598133 45.24696 60-69
GCTGG 13943705 3.5510392 32.79993 90-99
CCTGT 10222255 3.498109 60.458538 25-29
GAGTG 11628425 3.4470046 39.59938 90-99
ACTCT 8149830 3.3711734 67.32157 20-24
AGCTG 10839955 3.3369584 37.761887 60-69
GGATT 8667460 3.3244498 56.376736 40-44
TATCT 6437440 3.3178015 48.29946 200-209
AGACA 9483250 3.296594 38.666225 170-179
TCCTG 9590905 3.2820578 62.95261 20-24
CTCCT 9039855 3.2125533 92.00698 20-24
CTGCA 10046695 3.2118025 47.135967 25-29
GGTCC 11949710 3.160363 137.59738 9
GTCTC 9151020 3.1315265 34.44165 30-34
CCATC 9419135 3.1270795 36.83781 45-49
CATCT 7557455 3.1261377 32.05318 170-179
CCTCA 9313870 3.0921323 81.70773 5
GTCCC 11104825 3.0499568 72.903984 10-14
GGAGT 10193280 3.0215857 37.840416 90-99
AAGAA 7388045 2.9893823 38.870693 180-189
AGGGA 10656915 2.9511516 40.86288 80-89
GAGCT 9539245 2.9365494 30.738924 60-69
AGTAG 8154795 2.9219985 29.16211 50-59
GGCTG 11327395 2.8847444 33.849724 90-99
TGTGC 8545335 2.815875 56.804108 25-29
GGAAG 10139650 2.8079088 30.755138 80-89
GTGTA 7286485 2.794769 37.29863 230-239
CAGGG 11656460 2.7732115 36.548992 80-89
GTGAA 7677640 2.7510254 39.415398 10-14
ATCTG 6900465 2.7485812 36.241604 200-209
CTATG 6833170 2.7217765 28.286781 50-59
ACCTT 6568230 2.7169454 72.31839 40-44
GACAA 7792295 2.7087793 24.33449 180-189
ATCTC 6541360 2.7058306 47.101433 170-179
GGGAA 9606755 2.6603377 34.32606 80-89
GAACA 7623465 2.6500902 34.500175 210-219
GAGTC 8490105 2.6135836 29.541595 150-159
CCCTC 9107840 2.5977638 27.718147 20-24
ATCAG 6937300 2.5814264 28.60623 45-49
ATGAA 5936390 2.5711968 27.405092 210-219
AGAAC 7394435 2.5704741 30.547832 190-199
CAGTG 8303030 2.5559943 75.024765 8
CACGG 10328310 2.5518072 43.83866 230-239
TGCAA 6780840 2.5232062 35.292538 200-209
TATAT 4200420 2.5198436 24.147009 240-247
AGGGC 10586150 2.518572 32.624622 150-159
CTACA 6482705 2.505116 19.800776 190-199
CCAAG 8335820 2.4895043 39.477085 180-189
CGCCA 9672375 2.4817276 39.08953 70-79
GTGGA 8300450 2.4604955 27.516022 100-109
AGGCT 7977785 2.4558716 20.672344 25-29
CTGTC 7150540 2.4469519 51.02305 30-34
ACGGC 9858175 2.4356513 48.576427 230-239
CACTG 7559165 2.4165702 20.43967 30-34
TCTGT 5657735 2.412314 25.890007 210-219
CTACT 5830160 2.411643 24.045591 50-59
GCAGC 9629210 2.3790815 34.250854 30-34
TATGC 5925545 2.3602529 22.005663 50-59
TCCAA 6021750 2.326989 44.19057 180-189
CCGCC 10552475 2.3261173 33.24416 70-79
GTGGT 7287825 2.31249 31.528435 120-129
CTTCA 5590420 2.312475 50.87262 45-49
TGGTG 7253525 2.3016062 36.071404 35-39
TGAAC 6148790 2.288015 37.391747 210-219
CTCTC 6421105 2.281911 95.25926 20-24
ACAGC 7640425 2.2818236 26.455408 210-219
GGGCT 8950500 2.2794213 42.061493 90-99
CAAAT 5033185 2.2639046 35.03227 200-209
GTATC 5672970 2.2596474 22.648186 200-209
CAGTA 6046105 2.249805 22.273033 45-49
GGCCG 10956740 2.2395153 25.336525 230-239
CAGGC 9058995 2.2381988 33.078014 70-79
TATCA 4608940 2.2191021 18.055143 170-179
ACAAC 6137995 2.2158334 19.498224 180-189
GCCTC 8039915 2.2081747 71.71277 4
TACAT 4527500 2.1798904 18.593245 190-199
GCTCC 7935310 2.1794448 33.141937 80-89
AATGA 5026100 2.1769278 25.837812 210-219
AAATG 5020015 2.174292 33.390995 200-209
CCTTC 6070295 2.1572413 55.484146 45-49
GCCTG 8108010 2.1443415 30.183022 210-219
GCTAT 5368885 2.138525 34.691475 50-59
GTGGG 8682085 2.1291144 29.723166 100-109
TCTAT 4087255 2.1065361 21.345701 110-119
GTAGT 5484245 2.103511 25.09158 120-129
GGGGG 11049790 2.094219 91.926704 5
AACTA 4608355 2.072818 38.485252 130-139
TGCAC 6479905 2.0715442 27.976177 25-29
GCAGA 7192555 2.0684524 24.027191 140-149
GCTGT 6247980 2.0588462 19.142117 230-239
TGCAG 6687840 2.0587764 43.283344 30-34
CCTCT 5736255 2.038531 48.0326 35-39
ATATC 4215510 2.0296738 14.763831 160-169
AGCAC 6762005 2.0194824 21.027796 130-139
CTCAC 6081980 2.01917 56.48267 20-24
AGAGT 5628680 2.0168495 31.930552 150-159
TGTGA 5249370 2.0134234 20.188982 210-219
AACAG 5789805 2.0126681 33.25641 210-219
TGAGC 6523425 2.0081632 18.704977 200-209
ATGGA 5562115 1.9929982 12.460519 100-109
TGGTA 5176905 1.9856288 31.349771 120-129
CATAT 4093075 1.9707245 15.553261 160-169
GGCTC 7411620 1.9601656 21.08055 40-44
CAGAG 6811690 1.9589218 31.728468 170-179
TCTCT 4395360 1.9462024 33.619877 35-39
TTCAG 4854755 1.9337375 47.114265 45-49
TCCGC 7030510 1.93094 41.212345 70-79
CCGTG 7297935 1.9300992 20.603853 230-239
GGTGG 7867035 1.9292394 19.11582 40-44
GGATC 6250770 1.9242294 18.731842 70-79
TCCAT 4646045 1.9218345 28.22862 45-49
GGTCT 5804390 1.9126736 35.916775 15-19
ACCAG 6384200 1.9066503 14.329537 190-199
CAGAC 6375275 1.9039847 25.934368 140-149
ACTAT 3950155 1.9019117 17.552977 130-139
GCACA 6353095 1.8973612 18.692245 130-139
CTGTA 4743465 1.8894088 24.2344 190-199
ACCCT 5682575 1.8865707 44.57191 10-14
GTCAC 5899725 1.886068 38.776585 160-169
CTATT 3655655 1.8840933 20.662825 110-119
CAGTT 4728180 1.8833203 25.676346 190-199
GCTGA 6096900 1.8768622 27.998161 200-209
TGTCT 4383785 1.8691341 52.24132 30-34
CCTGC 6802010 1.8681822 50.242897 20-24
TGGGA 6289290 1.864329 16.94766 100-109
GCCAG 7507970 1.8549883 34.943657 70-79
TGGGG 7520380 1.8442285 119.71344 4
GATGG 6213000 1.8417146 21.09583 100-109
CCAGA 6139115 1.8334553 33.216465 170-179
AGAGA 5426500 1.8164598 27.65839 170-179
CAAGG 6281005 1.8063062 17.609259 80-89
AGGAC 6279655 1.8059183 20.319365 220-229
TTCCA 4357465 1.8024634 23.028599 180-189
CACAG 6031510 1.801319 16.098997 140-149
TCAGC 5624600 1.798114 11.455623 45-49
TACAA 3989655 1.7945294 18.941969 130-139
AGTCA 4782475 1.7795982 29.806784 160-169
ATACT 3695550 1.7793248 26.774858 130-139
TAGTG 4634935 1.7777536 26.550793 120-129
AGTTC 4435450 1.7667208 23.034761 190-199
GAGGA 6354985 1.7598456 26.659086 220-229
ACCTG 5504450 1.7597035 31.527056 25-29
GCCGA 7093255 1.752525 18.884699 220-229
TGACC 5429755 1.7358245 15.062198 210-219
CTGAA 4664485 1.7356932 24.327566 200-209
GGAGC 7261770 1.7276623 13.173589 200-209
AGCTA 4632565 1.7238154 26.2892 50-59
TAGTA 3701270 1.716029 16.791128 120-129
TTCTG 4022770 1.7152064 43.630516 30-34
ATGCA 4608225 1.7147584 17.055586 60-69
GCAAA 4907575 1.705985 31.899965 200-209
CATGA 4573190 1.7017215 22.944368 60-69
CTCAG 5304885 1.6959053 78.21541 6
CCTGG 6410500 1.6953975 32.489334 2
GTGCA 5489350 1.6898346 49.86123 25-29
ATTGG 4404430 1.6893423 21.936285 100-109
GGATG 5584480 1.6554029 22.405766 100-109
AACAC 4489500 1.620722 22.181213 190-199
GCCGT 6110570 1.6160743 29.805864 230-239
CTCCC 5627960 1.6052226 14.433291 190-199
CAGCT 5008720 1.6012249 13.0399065 50-59
GCACT 4991240 1.595637 26.965467 25-29
AGGGG 6942135 1.5904026 32.618748 90-99
GGGCC 7706195 1.5751163 28.22806 2
GTTCT 3693805 1.5749443 24.33011 190-199
TCTGC 4594205 1.5721607 32.66659 200-209
TCTCA 3796390 1.5703751 26.22962 100-109
CGATT 3941545 1.5699894 34.84197 160-169
TTCTC 3532750 1.564251 23.128489 190-199
CGTCC 5691445 1.5631639 19.726694 180-189
GGGGC 7933665 1.561508 28.193167 90-99
TCTGA 3919665 1.5612742 21.411419 220-229
AGTTA 3365055 1.5601491 16.661217 50-59
GGCTT 4667275 1.5379694 21.771833 25-29
CATCA 3978600 1.5374529 33.191677 45-49
ACAAT 3416975 1.5369405 23.01825 180-189
GAGCA 5301030 1.524483 7.890737 200-209
CCATG 4738525 1.5148469 15.787301 160-169
GTTAC 3786870 1.5083796 17.05011 50-59
CGGCC 7086150 1.5041298 31.565374 230-239
AGTGA 4195925 1.5034697 87.07159 9
CTGGT 4442230 1.4638121 32.98874 35-39
TTAGT 2945395 1.4617679 25.363201 110-119
CTTTA 2830460 1.4587948 41.965393 45-49
GTCCG 5503935 1.4556365 33.5331 70-79
GTGAC 4688670 1.4433542 16.046986 210-219
CAACC 4643565 1.4401854 15.747408 140-149
CTCAA 3725145 1.4395103 19.139547 150-159
GTCCA 4489300 1.4351729 29.923738 180-189
GGCAG 5989535 1.4249822 13.703912 150-159
ACTCC 4288685 1.4238099 16.819868 140-149
AAGCT 3804455 1.415669 17.842737 200-209
AACCC 4546510 1.4100841 21.000744 140-149
AAGAG 4179015 1.3988781 20.281246 150-159
CGTGT 4240070 1.3971959 24.669401 230-239
TAGCA 3751625 1.3960105 12.020538 130-139
TACGC 4351965 1.3912686 24.06351 140-149
CAACT 3589330 1.387027 18.735407 130-139
AGCAG 4770705 1.3719707 12.722033 50-59
ATCTA 2836330 1.3656298 21.786201 110-119
ACAGT 3655050 1.3600742 13.163311 120-129
CACGT 4252240 1.3593878 17.145687 180-189
GGGGT 5540650 1.358738 114.16014 7
GGCCT 5126815 1.3558987 68.82261 3
TCAAG 3602785 1.3406259 24.31131 150-159
CGACA 4484100 1.3391831 16.432547 70-79
CCATA 3457305 1.3360087 15.919802 160-169
TTACA 2774080 1.3356578 10.50266 120-129
GAGAG 4814610 1.3332794 25.401693 220-229
TGTCC 3892055 1.3318814 55.937447 15-19
TGGAA 3710080 1.329383 19.886574 120-129
GTGGC 5210950 1.327071 14.92099 40-44
CTACG 4146350 1.325536 16.058565 130-139
AACCA 3656995 1.3201854 17.94682 190-199
GAACT 3545045 1.3191403 14.298812 190-199
GTGCG 5179315 1.3190145 17.521208 240-247
CAATT 2733960 1.3163407 24.35334 180-189
CCAGT 4082230 1.3050376 19.541714 190-199
ATACA 2895495 1.3023809 22.597498 35-39
TGCGA 4209695 1.2959074 14.752953 240-247
ACAGA 3725190 1.294961 20.358032 140-149
ACATG 3474585 1.2929214 14.5442915 190-199
CCTTT 2909095 1.2881055 48.27997 45-49
TGTGT 3136990 1.2879591 15.000272 240-247
ATCCG 4026945 1.2873636 24.813595 70-79
GATTG 3348635 1.2843865 25.088114 100-109
AATTC 2658470 1.2799939 24.377838 180-189
AGAGC 4447200 1.2789365 24.465338 220-229
AGACC 4275640 1.2769259 103.477234 9
ACGTC 3981125 1.2727156 19.041428 180-189
TCACT 3045130 1.2596167 12.882222 190-199
TCCGT 3671580 1.2564338 13.663029 150-159
AAAAA 2559755 1.251977 7.7829943 190-199
AGTCG 4051780 1.247295 17.056017 150-159
TTATT 1941550 1.2467818 19.443655 240-247
ACATA 2762860 1.2427223 20.953545 130-139
ATATT 2049195 1.2293175 25.784369 240-247
ATCAT 2551080 1.2282882 16.21972 110-119
CTTCT 2772090 1.2274417 23.738064 30-34
CCTAC 3696545 1.2272242 16.986822 190-199
TCGGA 3967470 1.2213411 97.78555 5
GATCC 3779965 1.2084073 24.624166 70-79
ATTAT 2010950 1.2063744 9.53194 50-59
CGGCT 4557935 1.2054459 20.669104 230-239
TGGAC 3899280 1.2003495 16.191784 80-89
GTCGA 3897435 1.1997815 13.26087 150-159
TGAGT 3104855 1.1908835 21.228788 90-99
CTTCG 3472595 1.1883401 109.48089 3
TACCA 3074295 1.1880018 10.794048 45-49
ACACA 3284520 1.1857209 7.6398873 130-139
GAAGT 3273000 1.1727703 13.880045 140-149
AGCTC 3653220 1.1678885 12.817444 210-219
GGGTG 4749685 1.1647689 14.70527 60-69
ATTAG 2511595 1.1644572 24.981997 110-119
GATCT 2921150 1.1635474 9.499249 220-229
AAGGC 4039185 1.1615983 29.373047 25-29
TCGAG 3766470 1.1594654 12.991985 150-159
TTGGG 3652510 1.158973 16.27143 100-109
GGACT 3736655 1.1502873 13.770378 90-99
CGCTG 4344785 1.1490738 12.310881 190-199
ATTCC 2760850 1.1420244 22.469603 180-189
GCCCC 5177980 1.1413995 12.511235 80-89
ACGCA 3820425 1.1409754 20.705976 140-149
GACCC 4433535 1.1375517 43.825897 10-14
GAACC 3805700 1.1365777 11.2242775 180-189
TTCGG 3448000 1.1361915 105.65073 4
GTATA 2447525 1.1347522 10.237855 240-247
AGGCC 4585140 1.132847 14.427029 2
GCAAG 3918190 1.1268023 17.080135 25-29
ACAAG 3231340 1.1232874 17.859041 80-89
GAAGC 3894125 1.1198819 13.845769 200-209
AGGTC 3634860 1.1189508 18.429647 10-14
CCCCT 3918495 1.1176441 10.237644 140-149
AGATC 2989790 1.1125255 12.846054 210-219
TAGAC 2975525 1.1072172 18.539524 170-179
GCACC 4287250 1.100018 11.382865 130-139
ATCAA 2444640 1.0995884 13.3617115 110-119
CTTGA 2754880 1.097319 22.478271 90-99
GCTTC 3196075 1.0937134 28.76522 2
TTGAG 2847865 1.0923135 20.994698 90-99
CGCAG 4371685 1.0801088 16.198063 140-149
TACAG 2890780 1.075683 9.675753 120-129
CCGAT 3360805 1.0744072 23.212816 160-169
GGAGA 3871015 1.0719756 87.77342 7
TGGCT 3251710 1.0715095 32.974915 40-44
CTATA 2209945 1.0640393 13.809718 50-59
GCTCT 3109225 1.063993 18.099602 210-219
ATGGG 3588465 1.0637258 19.902163 100-109
CAGCA 3544910 1.0586923 12.518925 45-49
CACAT 2737980 1.0580393 13.857394 130-139
GTAGA 2911490 1.0432353 18.174923 170-179
ATGGT 2705620 1.0377548 9.999384 35-39
GGGAG 4524955 1.0366408 14.897227 5
ACACC 3334690 1.0342424 13.175574 40-44
CTCCG 3751155 1.0302603 9.666942 140-149
TAGTT 2074110 1.0293585 15.475419 50-59
ATGAG 2863635 1.0260879 20.004507 60-69
ACCAA 2840050 1.025266 17.999414 130-139
GTAGC 3319355 1.0218261 11.377103 50-59
GACAG 3549640 1.0208138 15.867965 70-79
ACCGC 3973500 1.0195162 11.153322 220-229
GAGCC 4108385 1.0150554 17.541233 220-229
CGAGT 3275390 1.0082921 15.414069 160-169
CGTGA 3270795 1.0068775 20.380835 150-159
AAACT 2228375 1.0023134 17.414331 130-139
CCAAC 3198585 0.9920299 14.899095 130-139
TGAGG 3346570 0.992021 11.898477 220-229
GGTAT 2582050 0.9903588 9.699419 110-119
TTTCA 1920425 0.98977053 6.9082956 100-109
TTATA 1649130 0.9893176 13.9234705 110-119
AAGGT 2746830 0.98423475 20.920494 15-19
ATGAC 2640520 0.9825591 11.085701 160-169
AGCCG 3948850 0.9756393 18.521778 220-229
ATGAT 2101540 0.9743422 9.439477 120-129
GGGAC 4094670 0.97417116 10.542665 90-99
CGAGG 4052685 0.9641824 14.217405 220-229
AGCCC 3737445 0.9589496 11.710228 80-89
TTTAG 1930060 0.95786804 29.937138 45-49
ACTCA 2463900 0.95212644 11.803814 190-199
AACTC 2462870 0.9517284 15.770248 190-199
CAGAA 2735140 0.9507969 18.491556 140-149
CCGTC 3453615 0.9485405 10.272983 140-149
GATAC 2545770 0.9473019 24.46596 35-39
GCCAA 3171025 0.94703126 14.435377 180-189
CGGAC 3819950 0.94379205 11.058838 220-229
CCCCA 3541750 0.94371563 17.487766 80-89
AATCA 2087325 0.9388696 14.626792 110-119
TATGG 2446480 0.9383603 13.000402 35-39
GCAGT 3047460 0.9381263 6.30309 50-59
CCCAG 3656125 0.9380846 15.4306755 80-89
ACAGG 3261030 0.9378147 15.38484 70-79
GACTG 3015630 0.9283278 13.4091425 90-99
TGGTT 2260655 0.9281606 9.711209 50-59
CCGAG 3732540 0.92219573 17.388681 220-229
CATAC 2384160 0.92131245 14.090712 130-139
GCCAT 2877585 0.9199279 7.7105656 60-69
TACAC 2374435 0.91755444 22.25852 35-39
AAGTT 1978920 0.9174916 18.01799 140-149
TAACA 2025980 0.9112769 8.49879 120-129
GAGAT 2527175 0.90552884 12.2205515 210-219
TCATA 1875770 0.90314156 8.922731 110-119
AACTG 2423325 0.9017391 11.192814 60-69
TCCTT 2024030 0.89621145 11.33583 1
CGGAG 3748580 0.8918321 75.60433 6
GTTAT 1788550 0.88763815 11.213002 110-119
AGAAG 2639485 0.8835378 16.212864 140-149
TTTAT 1372145 0.88113385 9.959659 240-247
CGTAT 2191685 0.87298816 7.55587 240-247
GGTAG 2904185 0.8608852 12.210191 120-129
ATAGT 1839550 0.8528751 15.888182 120-129
ATATG 1838695 0.85247874 6.311078 50-59
TTACC 2060045 0.8521367 9.780371 45-49
GATCA 2278155 0.8477202 9.449142 110-119
CATGG 2737455 0.8426947 8.721312 200-209
GTCTA 2112435 0.8414215 10.362563 30-34
ATCCA 2173215 0.83979684 9.730272 180-189
GATGA 2341465 0.83898586 6.4394245 60-69
GCCTA 2618350 0.8370537 16.36896 190-199
ACGCT 2615805 0.83624 15.160298 190-199
TATTT 1299350 0.83438796 9.980188 240-247
GCTTG 2514810 0.828685 17.239334 90-99
TCTTC 1871170 0.8285272 48.13036 2
TGGCA 2689500 0.8279323 5.9468865 100-109
AGTCT 2069780 0.8244312 15.477993 8
CTATC 1986775 0.8218286 8.136677 110-119
CCTCC 2867515 0.8178807 11.24369 140-149
GGTGC 3201955 0.8154411 11.723933 60-69
GGTTC 2465905 0.8125697 11.121774 160-169
ACATC 2100500 0.8116975 6.1459446 110-119
GTCTG 2462720 0.81152016 13.811784 1
TCATC 1933055 0.79960734 10.2742 110-119
ACAAA 1900020 0.7983851 12.884372 130-139
CATTA 1639810 0.789532 11.90635 110-119
TGATG 2036970 0.78129053 11.988624 120-129
ATATA 1392775 0.7805501 10.358223 130-139
TTATG 1551665 0.77007467 10.989006 50-59
GGTTA 2000085 0.76714313 8.644111 50-59
TATAC 1591955 0.7664909 8.662234 130-139
CCAGC 2962400 0.76008934 6.267585 80-89
TGACT 1896260 0.755315 5.0126815 2
CTGAC 2361800 0.7550378 9.67965 220-229
CCCCC 3296245 0.7545692 13.75888 80-89
TCAAC 1939565 0.74950737 8.114756 110-119
CATCC 2246815 0.745925 6.658561 110-119
GCGGA 3130110 0.7446907 9.423713 220-229
TTGGA 1930430 0.7404264 6.456431 60-69
GGGAT 2491855 0.73865855 13.344472 100-109
GTTCA 1853350 0.7382232 13.486089 160-169
GATTA 1586205 0.7354163 5.8686304 50-59
GACCG 2968885 0.7335201 9.85319 210-219
TCCGG 2754505 0.72848934 12.4652 70-79
ATAAA 1381265 0.7231625 8.320841 130-139
ATCTT 1401890 0.722522 13.453355 3
CGCCG 3392495 0.72010225 11.15033 220-229
GGGCA 2988575 0.71101785 13.683081 150-159
TTTAC 1373600 0.7079417 13.28208 45-49
GGATA 1966885 0.7047676 15.715524 35-39
ATGCC 2203725 0.7045032 8.058856 50-59
GACCA 2356165 0.7036721 6.3233542 170-179
AACGC 2354225 0.70309263 14.136307 180-189
TGCCA 2190420 0.70024985 7.409275 60-69
CACAA 1937305 0.69937253 8.521155 130-139
TATTG 1406130 0.69784725 6.397297 240-247
ACGCC 2711125 0.6956175 12.541271 180-189
AATAC 1522000 0.68458897 11.463758 130-139
CAAAC 1896340 0.6845841 13.891868 130-139
GAAAT 1571930 0.6808417 7.577139 110-119
CAACG 2276870 0.6799904 14.39016 180-189
AAATA 1285270 0.67290425 12.703812 130-139
GCCGC 3166815 0.6721987 12.264351 220-229
TATAG 1440645 0.66792995 6.1064796 120-129
TTTCT 1206860 0.66581726 9.876431 4
AGGCA 2310475 0.66445196 6.754504 80-89
AATAA 1260430 0.65989923 7.7397685 130-139
CAGTC 2045595 0.653951 5.293411 210-219
GCGAC 2634630 0.65093595 7.2383804 70-79
CCCGT 2368890 0.65061915 10.012901 140-149
CCGGC 3046370 0.6466326 10.093007 70-79
CACGC 2511840 0.6444852 12.197777 190-199
TCAAT 1322865 0.6369301 8.856137 110-119
ACTTC 1531250 0.63340086 30.016596 4
GACGA 2201730 0.63317883 9.845584 220-229
TCCAC 1898745 0.6303686 10.372056 180-189
TACTT 1207505 0.6223377 11.201923 1
CCCTA 1861400 0.61797035 12.120978 110-119
TAGCT 1538840 0.6129481 12.640555 50-59
CACCG 2386765 0.61239356 9.58651 45-49
AAGCA 1760955 0.6121481 5.969379 120-129
AGATG 1707320 0.61176115 5.5223923 120-129
AGTAA 1409265 0.61038744 5.173547 50-59
GGCCC 2873635 0.60996735 10.483049 70-79
CACGA 2038835 0.60890096 8.9141865 180-189
CTTTG 1387920 0.59177375 7.346 45-49
AATGG 1645875 0.5897443 6.629286 120-129
TGATT 1185245 0.58822435 6.319692 50-59
ACGAC 1943485 0.5804245 9.182856 220-229
ATTAA 1034990 0.5800375 6.1981153 110-119
CGGCA 2337550 0.57753664 11.641305 70-79
CCGTA 1804370 0.5768345 6.4240766 230-239
ACCCG 2246065 0.5762929 8.761256 140-149
TCAGG 1864595 0.57399464 5.717982 150-159
GGGGA 2501425 0.5730618 9.045154 4
ACCTA 1482855 0.57302064 7.046788 130-139
GTTCC 1668180 0.57085985 6.631202 140-149
GGTGA 1910895 0.5664451 6.116737 120-129
GAAGA 1687525 0.56488 6.2306576 15-19
TAATA 1007220 0.5644743 8.445225 130-139
CTGCG 2110475 0.5581615 12.218994 25-29
TTAGC 1398015 0.5568549 9.500227 45-49
ATTTC 1066340 0.5495825 5.9208374 240-247
CGGTT 1656425 0.5458283 11.045677 160-169
ACCCC 2040320 0.54365265 9.619435 140-149
GCTAC 1691785 0.5408424 8.2071905 50-59
AAATC 1196520 0.5381895 8.15485 110-119
TTCAA 1113995 0.5363638 5.225722 200-209
GTCCT 1567095 0.5362681 6.5836554 45-49
GGAGG 2326065 0.5328879 5.9794345 6
CCACG 2076610 0.5328143 7.779724 180-189
CCTAT 1281650 0.53015393 5.1769705 110-119
GGGTA 1769730 0.5245996 5.6536627 100-109
AAGAT 1199380 0.51948106 5.5946836 15-19
AAGTA 1183360 0.51254237 5.104402 120-129
AGTTT 1021545 0.50698185 5.221534 140-149
TATGT 1018515 0.5054781 6.3421326 140-149
CTGTT 1173185 0.5002161 5.6163235 240-247
CGCGG 2382070 0.4868859 6.611259 220-229
TGACG 1574530 0.48470134 8.815025 220-229
GTAAC 1298535 0.48319548 6.695713 120-129
GGGTT 1510225 0.47920752 5.465426 100-109
TAAAT 852635 0.47784057 8.291645 130-139
ACATT 986270 0.474867 5.8545012 110-119
TATCC 1129500 0.46721718 5.3748665 110-119
ATGCT 1159650 0.4619098 7.2831907 50-59
TGGCC 1734285 0.4586697 14.444963 2
CAATC 1183650 0.4573986 6.622256 110-119
TGCTA 1142905 0.45523995 6.477746 50-59
CCGGT 1710715 0.45243612 8.520717 160-169
CCGCG 2126750 0.45143104 6.8407454 220-229
AACAT 996225 0.44809765 5.5586405 110-119
GGTTT 1087200 0.44637346 5.1206822 100-109
TTGGT 1065425 0.43743318 5.212557 120-129
TGCGC 1652015 0.4369116 9.0763445 25-29
GGTCA 1403145 0.43194243 5.5070014 160-169
CATAA 959980 0.43179482 5.7121725 110-119
ATTCT 836180 0.43096003 8.190098 2
GCCTT 1245945 0.4263689 8.833606 1
GCGCT 1599990 0.42315248 8.246863 25-29
GCATG 1348985 0.41526988 8.260026 60-69
CTAAC 1058710 0.409118 5.5997696 110-119
ACGAG 1415725 0.4071375 7.848863 180-189
CCTAA 1038440 0.40128508 7.7829943 110-119
CACTT 966810 0.39992052 6.989757 3
ATGGC 1291460 0.39756146 6.8901954 1
CCGGG 1925820 0.39363018 5.16984 80-89
CATGC 1227565 0.3924371 7.1065226 60-69
TTTGA 786690 0.3904258 6.156526 45-49
AGCTT 974315 0.38808748 7.2852025 1
TACCT 934940 0.3867375 5.103949 2
GGTAA 1061475 0.38034415 7.040201 120-129
GAGGC 1551015 0.36900508 5.8706236 35-39
TTCTT 657065 0.36249876 20.060837 1
TGGGC 1384310 0.3525418 9.573262 1
TGCTG 1041845 0.34331074 14.06961 1
CGAGC 1383160 0.34173626 6.6003633 180-189
AACCT 828695 0.32023314 5.44203 2
CTCTT 693110 0.30689916 9.442146 1
AACTT 637385 0.3068867 10.868486 3
CTCGG 1156635 0.30589753 5.2923374 6
GCGAG 1262655 0.30040076 5.3887897 240-247
CGCTT 857455 0.29342556 6.381142 1
TCACA 746280 0.28838548 5.646062 7
GACTT 720660 0.28705204 6.778089 1
TTCCT 642580 0.2845252 6.159177 4
GAGGT 932490 0.27641726 7.4941506 7
TAACT 570540 0.27470228 8.069235 2
ATTGT 553475 0.2746837 5.2571263 240-247
CCCTT 736910 0.2618806 5.92036 3
GTCTT 597615 0.2548078 12.806113 1
TGTAA 541100 0.25087157 7.664921 25-29
CGGGG 1262680 0.24852133 8.970573 3
TTGGC 750680 0.24736547 7.2015963 1
AATCT 502575 0.24197865 7.3877296 2
CCTCG 863895 0.23727006 5.7457204 5
TCGGT 705660 0.23253042 6.3189063 7
TGCTT 522310 0.22269966 11.599759 1
TAGGC 523200 0.16106124 6.960589 1
TCGCA 498160 0.1592555 5.5242 7
TTGCT 288030 0.12280864 5.570156 4
Produced by FastQC (version 0.10.1)