FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_18_SL248347.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_18_SL248347.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5363659
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23194 0.432428683478946 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19310 0.36001542976538964 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18967 0.3536205414997486 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18855 0.3515324147191311 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 16507 0.30775632828261457 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15609 0.2910140260594493 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15087 0.28128186374264286 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14763 0.2752412112701422 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 14626 0.2726869847617084 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14420 0.2688463230045012 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14164 0.26407346179166125 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14082 0.26254465468442345 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14014 0.26127686342476286 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13889 0.25894636478568084 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13887 0.2589090768074555 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13752 0.25639213827724694 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13464 0.251022669412802 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13421 0.25022097788095776 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13390 0.24964301421846544 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13179 0.24570913251569498 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12919 0.2408616953464044 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12836 0.23931424425005393 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12757 0.23784136911015408 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12324 0.22976852182437396 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12321 0.22971258985703602 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11524 0.2148533305342491 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11124 0.20739573488918667 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10667 0.19887543186470283 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10527 0.19626527338893096 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10462 0.1950534140966083 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10403 0.1939534187389616 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10401 0.19391613076073627 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10398 0.19386019879339833 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10199 0.19015004495997975 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9943 0.18537718374713977 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9775 0.18224499357621357 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9691 0.18067889849075044 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9658 0.1800636468500328 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9626 0.1794670391984278 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9583 0.17866534766658357 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9474 0.17663315285330405 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9332 0.17398570639930688 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9274 0.17290435503077284 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9200 0.17152469983643628 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9168 0.1709280921848313 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9079 0.1692687771538049 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 8687 0.1619603334216437 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 8678 0.1617925375196298 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8596 0.160263730412392 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 8210 0.15306715061490672 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8097 0.15096037984517657 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8023 0.14958072465084002 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 7708 0.14370786808035335 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7502 0.1398672063231462 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 7388 0.13774179156430338 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 7360 0.13721975986914903 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 7339 0.13682823609778325 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 7269 0.13552315685989733 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7190 0.1340502817199975 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7073 0.13186893499381672 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6923 0.1290723366269183 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 6835 0.12743166558500457 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 6820 0.12715200574831473 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6744 0.12573506257575287 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6213 0.11583510435693245 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6208 0.11574188441136918 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 6022 0.11227410243641514 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 5940 0.11074529532917733 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 5474 0.1020571964026796 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 5392 0.10052838929544179 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 10802895 7.609411 66.37932 35-39
TATTA 5393965 7.266752 126.47383 240-247
TGGAT 8183470 7.121833 78.73535 35-39
TCACC 8351440 6.4173684 74.57517 160-169
TCTCC 7440020 6.149834 87.53764 20-24
CTGTG 7824770 5.928928 87.547424 25-29
TCCCT 7029370 5.8103957 72.973114 15-19
AGTGG 8429710 5.6850085 39.898254 100-109
CTGAG 7954620 5.603125 50.448154 15-19
TGTAT 4937595 5.5450506 59.84241 240-247
TTACT 4497000 5.2747927 111.52408 240-247
ATTAC 4562690 4.9751906 104.72744 240-247
TGGGT 6776870 4.9163327 45.47376 100-109
CCTGA 6623755 4.8731246 52.15655 15-19
CTCTG 5981240 4.733563 74.07765 35-39
TTCAC 4932725 4.6830196 74.414 40-44
CTCCA 6002730 4.6125855 41.56792 80-89
ACACG 6719030 4.5953236 49.198856 230-239
GACAC 6712485 4.590847 46.768085 230-239
TGAGA 5468440 4.424091 93.46958 15-19
ATTCA 3970335 4.3292828 111.69764 40-44
TCTGG 5700935 4.319671 134.97188 35-39
TACTG 4704395 4.2761254 78.53133 240-247
AAGGG 6779335 4.2502246 39.10107 90-99
CCCTG 6532225 4.184221 85.166885 10-14
AGCCT 5655735 4.160948 50.862576 30-34
CAGCC 6985370 4.1595774 55.695393 30-34
TGGAG 6106770 4.1184144 38.382362 90-99
CCAGG 7214760 4.1132894 53.180866 80-89
GAAGG 6551425 4.1073394 30.856396 150-159
ACCAT 4591765 4.0525184 66.71827 160-169
GAGAC 6136940 4.018547 84.540596 8
TCCAG 5416100 3.9846473 40.893875 80-89
TCAGT 4380790 3.9819806 95.46034 7
GTATT 3509425 3.9411776 69.1559 240-247
GGGTC 6703100 3.9359035 124.88265 8
CACCA 5478760 3.9136698 56.993237 160-169
ACTAC 4425065 3.9053955 34.863544 130-139
TACTA 3518445 3.8365388 28.46508 130-139
CTGGG 6471575 3.799957 132.42885 3
CACCT 4936780 3.7934937 43.96594 40-44
GACTC 5130850 3.7747877 52.47801 20-24
GATTC 4104310 3.73067 59.47616 40-44
ACTGT 4102900 3.7293882 67.52227 240-247
AGACT 4385205 3.7054665 68.59451 15-19
TGAAG 4565665 3.6937258 39.39964 150-159
GGACA 5511460 3.608975 27.963106 220-229
GCTGG 6083980 3.57237 33.36626 90-99
CAAGA 4529550 3.5580688 43.357758 180-189
CCTGT 4459885 3.5295603 62.80093 25-29
ACTGG 4971530 3.5018773 43.78088 60-69
ACTCT 3637565 3.453423 69.01628 20-24
GAGTG 5061800 3.413685 38.594913 100-109
TCCTG 4254630 3.367121 64.13711 20-24
TATCT 2847110 3.3395402 49.267574 200-209
CTCCT 3983905 3.2930496 94.57632 20-24
GGTCC 5322570 3.2642426 142.46912 9
GGATT 3730680 3.246701 56.723938 40-44
AGACA 4129510 3.2438283 37.209538 170-179
CTGCA 4393645 3.2324233 44.80864 25-29
AGCTG 4555840 3.2090707 36.40426 60-69
CATCT 3358345 3.1883378 33.428745 170-179
GTCTC 3947460 3.1240263 32.798035 30-34
CCATC 4058680 3.118749 34.645557 45-49
GTCCC 4806675 3.078919 75.636345 10-14
CCTCA 3895775 2.9935703 81.32437 5
GGAGT 4426875 2.9854908 36.787624 90-99
GGCTG 5013655 2.9439006 34.470024 90-99
AAGAA 3211735 2.8976688 36.205956 180-189
AGGGA 4570595 2.865481 39.568913 80-89
ATCTC 3005730 2.8535733 48.48693 170-179
GTGTA 3272590 2.8480387 35.720695 230-239
AGTAG 3504035 2.8348432 28.127708 50-59
TGTGC 3717140 2.8165243 57.419422 25-29
GAGCT 3983775 2.8061163 29.299294 60-69
ATCTG 3062305 2.783525 37.172234 200-209
GTGAA 3431670 2.776298 38.65345 150-159
GGAAG 4378345 2.7449522 29.576027 80-89
GACAA 3471425 2.7268865 24.767616 180-189
CAGGG 4973005 2.7145255 35.946205 80-89
ACCTT 2837820 2.694163 71.31841 40-44
GAACA 3421505 2.6876736 35.019978 210-219
CTATG 2946355 2.6781306 28.010529 50-59
GGGAA 4160275 2.6082356 33.15786 80-89
ATGAA 2682075 2.602997 27.753208 210-219
CACGG 4516820 2.575136 44.472763 230-239
CGCCA 4287300 2.552958 40.030437 70-79
TGCAA 3010060 2.543479 35.7545 200-209
GAGTC 3594270 2.5317545 27.18395 150-159
CCCTC 3769915 2.5221877 27.505554 20-24
AGAAC 3204070 2.5168731 30.54296 190-199
AGGGC 4591540 2.5063019 32.588272 150-159
CAGTG 3533085 2.4886563 73.95752 8
ATCAG 2917305 2.4651015 26.196089 45-49
AGGCT 3498385 2.4642143 20.257103 80-89
TATAT 1827705 2.4622846 24.490726 240-247
ACGGC 4303315 2.4534123 48.881985 230-239
CCAAG 3574380 2.4446137 38.155888 180-189
CTACA 2738490 2.416888 19.450354 190-199
GTGGA 3542450 2.3890333 26.256783 100-109
CACTG 3239995 2.3836777 19.488401 30-34
TCTGT 2436945 2.3827915 24.240309 210-219
CTCTC 2867650 2.370366 98.61948 20-24
CCGCC 4570905 2.3698032 34.422398 70-79
GCAGC 4146035 2.3637435 34.69409 30-34
TGAAC 2793620 2.3605886 38.054287 210-219
GTATC 2587305 2.351767 23.211573 200-209
CTGTC 2965735 2.3470874 48.120537 30-34
ACAGC 3425170 2.342565 27.078405 210-219
CTACT 2455785 2.3314676 22.661568 50-59
TATGC 2557430 2.3246114 21.222513 50-59
GGGCT 3929095 2.3070724 42.586376 90-99
GTGGT 3175850 2.303945 31.111177 120-129
CTTCA 2421425 2.298847 50.65415 45-49
CAAAT 2256845 2.2876883 34.79012 200-209
GGCCG 4800210 2.2813168 25.16714 230-239
TGGTG 3135820 2.2749052 33.53377 35-39
TCCAA 2570595 2.2687101 42.310455 180-189
CAGGC 3978360 2.2681484 33.213398 70-79
GCCTC 3511365 2.249207 71.91301 4
GCCTG 3637380 2.2307441 31.099451 210-219
AATGA 2277355 2.2102096 26.127214 210-219
GCTCC 3447810 2.2084968 34.381817 80-89
TATCA 2014320 2.1964295 16.88709 170-179
AAATG 2262055 2.1953607 33.01783 200-209
AAAAA 2023550 2.1901057 17.39686 90-99
ACAAC 2664000 2.1856766 19.727911 180-189
CAGTA 2559270 2.1625645 21.061377 45-49
GGGGG 4956080 2.1591303 94.41913 5
CCTTC 2596130 2.1459308 55.896534 45-49
TACAT 1959450 2.1365988 18.193855 190-199
GTGGG 3761685 2.1147487 30.165941 100-109
GCTAT 2325850 2.1141136 33.991028 50-59
CCTCT 2540390 2.0998569 49.46038 35-39
TGCAG 2975215 2.0957005 43.668736 30-34
GTAGT 2403860 2.0920086 25.067802 120-129
GCTGT 2748740 2.082755 19.364761 230-239
GCAGA 3169685 2.0755503 24.394844 140-149
TCTAT 1739445 2.040296 19.810066 110-119
AACAG 2594745 2.0382338 33.591225 210-219
CTCAC 2647970 2.0347388 54.98497 20-24
TGCAC 2745035 2.0195339 25.97245 25-29
CAGAG 3033820 1.9865841 32.481327 170-179
AGAGT 2449870 1.982 29.566988 150-159
GGCTC 3226015 1.9784609 19.660786 40-44
AACTA 1945080 1.9716625 35.898136 130-139
AGCAC 2876800 1.9675201 20.384853 130-139
TGTGA 2257245 1.9644139 18.512314 210-219
TCCGC 3061105 1.9607929 42.37031 70-79
ATGGA 2415425 1.9541332 12.002609 100-109
TGGTA 2241640 1.9508332 30.76955 120-129
TTCAG 2142825 1.9477508 47.68044 45-49
CCGTG 3168660 1.9432863 19.915407 230-239
TGAGC 2758800 1.9432608 17.434355 200-209
TCTCT 1896850 1.937161 32.836044 35-39
CAGAC 2831910 1.9368186 26.69613 140-149
CTGTA 2130730 1.9367568 24.439552 190-199
GGTCT 2548710 1.9311898 35.74761 15-19
ATATC 1764270 1.923773 13.372817 170-179
CATAT 1761850 1.9211342 13.794845 160-169
GGTGG 3387355 1.9043072 18.104635 40-44
CCAGA 2767690 1.8928969 34.118977 170-179
GCCAG 3312990 1.8888067 35.632793 70-79
GGATC 2662990 1.8757735 17.929693 70-79
TGGGG 3332525 1.8734831 122.75083 4
GCACA 2735320 1.8707582 18.363438 130-139
AGAGA 2474035 1.8606845 27.892424 170-179
ACTAT 1704775 1.8588992 17.193584 130-139
AGGAC 2829280 1.8526489 20.564207 220-229
CCTGC 2890530 1.8515309 49.30687 20-24
TCCAT 1950220 1.8514954 26.868774 45-49
ACCCT 2396640 1.8416132 43.18745 10-14
ACCAG 2688245 1.8385624 13.882315 190-199
CTATT 1566740 1.8377203 19.548471 110-119
GATGG 2724705 1.8375449 20.846867 100-109
GTCAC 2492890 1.8340297 37.568172 160-169
TCAGC 2478420 1.823384 11.554577 45-49
CAGTT 2004195 1.821741 24.18086 190-199
TTCCA 1906390 1.8098843 23.094378 180-189
GCTGA 2567880 1.8087792 26.531122 200-209
CACAG 2641725 1.806746 15.781424 140-149
TGGGA 2677030 1.8053929 16.49493 100-109
GCCGA 3157405 1.8001045 19.429808 220-229
GAGGA 2866410 1.797062 26.821747 220-229
TGTCT 1835380 1.7945943 48.6837 30-34
ATACT 1632365 1.7799429 26.54114 130-139
CAAGG 2689675 1.7612336 17.066154 80-89
TAGTG 2016585 1.7549745 25.966862 120-129
TAGTA 1672035 1.7455881 16.935373 120-129
ACCTG 2369385 1.7431663 30.698265 25-29
GCAAA 2212680 1.7381126 32.144295 200-209
TTCTG 1774295 1.7348667 42.843136 30-34
CCTGG 2822480 1.7309798 33.898483 2
AGTCA 2023085 1.7094922 28.298574 160-169
TACAA 1682235 1.7052253 17.063814 130-139
CTGAA 2017175 1.7044983 22.657532 200-209
CTCAG 2308925 1.6986856 77.48625 6
TGACC 2308385 1.6982886 14.11813 210-219
AGTTC 1868310 1.6982266 21.37514 190-199
CATGA 2005015 1.6942232 23.105392 60-69
AGCTA 1994335 1.6851985 25.858835 50-59
GTGCA 2389810 1.6833494 50.068707 25-29
ATGCA 1991840 1.6830903 16.436676 60-69
AACAC 2031725 1.6669271 22.861523 190-199
GCCGT 2704300 1.6585019 29.150316 230-239
GGATG 2447780 1.6507863 22.210672 100-109
GGAGC 3024045 1.6506814 13.133431 200-209
TCTGC 2057205 1.6280754 33.865925 200-209
TCTCA 1714140 1.6273664 26.375284 100-109
ATTGG 1859650 1.6183985 19.980549 100-109
AGGGG 3074300 1.6066782 32.770878 90-99
CAGCT 2177870 1.602268 13.035444 50-59
GGGCC 3357350 1.5955923 27.918646 2
CGATT 1749155 1.5899189 35.116516 160-169
TCTGA 1746355 1.5873739 21.289606 220-229
GGGGC 3478050 1.5825912 28.764505 90-99
ACAAT 1540095 1.5611427 23.275139 180-189
CTCCC 2317400 1.5504111 13.752513 190-199
GCACT 2090670 1.5381145 24.951555 25-29
GTTCT 1568120 1.5332733 22.745878 190-199
CGTCC 2385405 1.5279727 18.684687 180-189
TTCTC 1488155 1.5197804 21.67174 190-199
CCATG 2063575 1.5181807 15.525942 160-169
CGGCC 3054500 1.5162046 31.797956 230-239
GGCTT 1992455 1.5097085 21.347893 25-29
AGTTA 1441920 1.5053502 15.448046 50-59
GTCCG 2452015 1.5037799 34.637924 70-79
AGTGA 1841500 1.4898149 84.54742 9
GAGCA 2269455 1.4860682 7.6097937 200-209
CTGGT 1950955 1.4782634 31.024866 35-39
TTAGT 1314330 1.4760277 25.438213 110-119
CTTTA 1248970 1.4649893 41.824295 45-49
CATCA 1656300 1.4617879 30.380274 45-49
ACTCC 1896435 1.4572483 17.278725 140-149
TACGC 1975620 1.453472 24.712585 140-149
GTTAC 1586915 1.4424486 15.339468 50-59
CGTGT 1858825 1.4084551 23.60704 230-239
GTGAC 1999140 1.4081668 14.800837 210-219
GGGGT 2488050 1.398735 117.0775 7
AAGAG 1849990 1.3913497 18.145792 150-159
GGCAG 2538950 1.3858914 13.404185 150-159
CTCAA 1569580 1.3852521 18.410297 150-159
GTCCA 1876275 1.3803835 28.459316 180-189
TAGCA 1633455 1.3802576 11.699597 130-139
GGCCT 2246465 1.3777194 68.669014 3
CTACG 1871405 1.3768005 16.13158 130-139
GAGAG 2173135 1.3624216 25.734293 220-229
AACCC 1901535 1.358333 19.824482 140-149
CAACC 1899095 1.35659 15.192048 140-149
AGCAG 2065925 1.352794 12.566573 50-59
CGACA 1957250 1.3386154 16.073294 70-79
CAACT 1513420 1.3356873 17.044432 130-139
CAATT 1224050 1.3347131 24.484762 180-189
GAACT 1579505 1.3346703 14.413577 190-199
AAGCT 1578885 1.3341463 16.110662 200-209
TGTGT 1411995 1.3218467 15.454879 240-247
TGGAA 1631085 1.3195844 19.339867 120-129
ACAGT 1559700 1.3179351 12.165616 120-129
ATACA 1298675 1.3164234 21.298096 35-39
CACGT 1779615 1.3092701 16.500298 180-189
ATCTA 1200505 1.3090396 20.225882 110-119
GTGCG 2228275 1.3083909 17.06019 240-247
AGAGC 1994935 1.3063091 24.708803 220-229
CCATA 1474015 1.30091 14.356887 160-169
TTACA 1193000 1.3008561 9.607457 120-129
ACAGA 1653660 1.298989 19.980305 140-149
TTATT 895230 1.2973602 20.345684 240-247
GTGGC 2207630 1.2962687 14.248278 40-44
CCTTT 1268365 1.2953196 48.37958 45-49
TCCGT 1635200 1.2940999 14.123507 150-159
TCACT 1361575 1.292649 13.333763 190-199
AATTC 1183465 1.2904589 24.449486 180-189
TGTCC 1628350 1.2886788 53.92431 15-19
TCAAG 1522770 1.2867297 22.66864 150-159
ACATG 1515635 1.2807004 14.343362 190-199
ATCAT 1173600 1.2797021 15.914414 110-119
CCTAC 1657380 1.273555 17.348934 190-199
ATATT 942140 1.2692513 26.231197 240-247
TGCGA 1790635 1.2612987 14.02558 240-247
AACCA 1533965 1.2585403 17.158728 190-199
ATTAT 927855 1.2500064 9.594415 50-59
AGACC 1826175 1.2489698 97.509224 9
ACATA 1225745 1.2424967 20.558893 130-139
CCAGT 1686270 1.2405958 18.494911 190-199
CGGCT 2013075 1.2345852 21.334944 230-239
CTTCT 1203800 1.2293826 23.177853 30-34
ACACA 1496555 1.2278472 7.6390033 130-139
ACGTC 1663415 1.2237813 18.227636 180-189
GATCT 1335510 1.2139305 9.308473 220-229
GATTG 1390050 1.2097195 23.099258 100-109
ATCCG 1643485 1.2091187 23.379986 70-79
TCGGA 1711535 1.2055818 91.06124 5
AGTCG 1709075 1.203849 15.583552 150-159
TACCA 1357780 1.1983254 11.0509 45-49
TGGAC 1682300 1.184989 15.765451 80-89
AGATC 1395735 1.1793859 12.361494 210-219
CGCTG 1918195 1.1763968 12.662916 190-199
TGAGT 1346850 1.1721239 20.603739 90-99
AAGGC 1780060 1.165606 28.309269 25-29
GTCGA 1653045 1.1643822 12.106991 150-159
ATTAG 1115285 1.1643465 25.099312 110-119
ATTCC 1219815 1.1580651 22.70435 180-189
GGGTG 2057190 1.1565136 14.468678 60-69
ACGCA 1688170 1.1545844 21.03106 140-149
CTTCG 1457190 1.1532226 103.161674 3
GATCC 1567135 1.1529479 23.21079 70-79
AGGCC 2010055 1.1459756 14.209196 2
GAAGT 1404740 1.1364663 13.2444725 140-149
TCGAG 1589560 1.1196642 11.813595 150-159
AGCTC 1517875 1.116707 12.194032 210-219
GACCC 1874260 1.1160654 42.70061 10-14
GAACC 1626955 1.112718 10.12068 190-199
GGACT 1576425 1.1104121 12.6553335 90-99
GCAAG 1695035 1.1099306 16.696367 25-29
AGGTC 1573150 1.1081052 17.966621 10-14
GTATA 1056670 1.1031531 10.208772 240-247
TTGGG 1519020 1.101985 14.905722 100-109
ACAAG 1401250 1.1007152 17.161957 80-89
CGCAG 1926910 1.0985727 16.637468 140-149
GCCCC 2111600 1.0947671 12.266222 80-89
CCGAT 1485465 1.0928628 23.559147 160-169
TTCGG 1439290 1.0905683 98.66456 4
CACAT 1230025 1.0855736 13.761077 130-139
GCTTC 1368165 1.0827678 26.58761 2
GCACC 1811810 1.0788783 10.99781 130-139
CCCCT 1604615 1.0735363 9.691115 70-79
CTTGA 1179980 1.0725594 21.976608 90-99
TTGAG 1231085 1.071377 20.3648 90-99
TACAG 1258860 1.0637275 9.208558 120-129
TAGAC 1257525 1.0625995 16.946915 170-179
CTCCG 1655070 1.0601562 10.016897 140-149
ATGGG 1570070 1.0588574 19.823866 100-109
CAGCA 1544175 1.0561024 12.271573 45-49
GAAGC 1612595 1.0559479 12.599981 200-209
ATCAA 1030955 1.0450445 12.1759405 110-119
ATGGT 1193155 1.0383676 9.369769 35-39
ACACC 1451165 1.0366179 12.765312 40-44
GTAGA 1281315 1.0366126 16.547884 170-179
GAGCC 1814380 1.034417 18.105803 220-229
CGTGA 1465280 1.032123 20.967924 150-159
CTATA 946510 1.0320816 13.190142 50-59
ATGAG 1275050 1.0315442 19.834183 60-69
GGAGA 1645065 1.0313542 80.72163 7
TGGCT 1360215 1.0306522 30.805357 40-44
GACAG 1545965 1.0123177 15.420778 70-79
GCTCT 1278200 1.0115694 16.932426 210-219
GTAGC 1436040 1.0115268 11.299233 50-59
ACCGC 1698575 1.0114503 11.01166 220-229
CGAGG 1851485 1.010637 14.636223 220-229
GGGAG 1929370 1.0083195 14.73484 5
AGCCG 1768140 1.0080546 19.070913 220-229
TTTCA 858190 1.0066208 7.2687073 100-109
ACTCA 1138045 1.0043956 12.298811 190-199
AAACT 986825 1.0003114 16.9315 130-139
TGAGG 1483140 1.0002317 11.81813 220-229
TAGTT 887740 0.99695563 14.390129 50-59
TTATA 735580 0.9909738 13.312323 110-119
ATGAC 1162500 0.9823041 10.769592 160-169
ATGAT 935990 0.9771643 9.058458 120-129
AACTC 1106065 0.97617126 16.144117 190-199
CCGTC 1508195 0.96607536 10.370864 140-149
CCGAG 1688415 0.9626018 17.991922 220-229
AAGGT 1187375 0.960613 20.2802 15-19
GCCAA 1403735 0.9600517 14.686222 180-189
GCAGT 1361830 0.9592542 6.3021774 50-59
CGAGT 1359245 0.9574334 14.847364 160-169
TTTAG 850820 0.9554935 29.380833 45-49
GCCAT 1295245 0.9529171 7.8121014 60-69
GGTAT 1094365 0.9523935 9.27134 110-119
GATAC 1126425 0.95182097 23.267534 35-39
ACCAA 1157480 0.94965345 16.421776 130-139
CGTAT 1039485 0.9448543 7.5797687 240-247
CGGAC 1646740 0.9388418 10.700075 220-229
CATAC 1057895 0.9336582 14.050329 130-139
GGGAC 1708055 0.93234545 9.720069 90-99
CAGAA 1184465 0.9304253 17.882956 140-149
TTTAT 641905 0.9302435 10.8695 240-247
TATGG 1064975 0.9268163 12.108218 35-39
CCAAC 1297325 0.9267246 13.599101 130-139
AGCCC 1553080 0.9248123 11.260028 80-89
ACAGG 1410835 0.9238328 14.922491 70-79
AACTG 1086810 0.9183465 11.531873 60-69
TCATA 838520 0.91432846 8.864233 110-119
TAACA 898335 0.9106121 8.70421 120-129
GAGAT 1121000 0.90691423 11.953046 210-219
AATCA 893515 0.90572625 13.585305 110-119
TACAC 1023365 0.90318334 21.359194 35-39
CCCAG 1514950 0.90210706 14.446963 80-89
TGGTT 959440 0.89818496 9.081794 50-59
ACGCT 1217010 0.8953593 15.492393 190-199
GTTAT 792295 0.88976836 10.872491 110-119
TCCTT 865240 0.8836276 10.498542 45-49
AAGTT 844280 0.88141996 17.120638 140-149
CCCCA 1417040 0.8813214 16.645226 80-89
TATTT 606605 0.87908703 10.852492 240-247
GACTG 1234815 0.8697866 12.290032 90-99
CGGAG 1592135 0.8690703 70.441216 6
CATTA 792750 0.86442053 12.22976 110-119
AGTCT 948605 0.86224777 16.296804 8
GTCTG 1137125 0.86161405 15.94004 3
AGAAG 1144460 0.8607311 15.516511 140-149
GATGA 1058115 0.8560388 6.534942 60-69
TTACC 901175 0.85555553 10.18351 45-49
GCCTA 1154990 0.8497309 16.648926 190-199
GATCA 1000075 0.8450561 9.323124 110-119
ATAGT 806810 0.8423016 15.290699 120-129
GGTAG 1248305 0.84185857 11.852916 120-129
TCTTC 823655 0.84115887 46.48278 2
GGTTC 1109095 0.8403753 11.49523 160-169
GTTTC 857405 0.83835185 5.055872 100-109
CATGG 1186695 0.83589166 8.594676 200-209
CTATC 878390 0.83392394 8.378983 110-119
ATCCA 943415 0.83262247 9.726236 180-189
ATATG 787190 0.8218186 5.628339 50-59
ATATA 655495 0.82093334 10.679373 130-139
TGGCA 1164435 0.820212 5.9990125 100-109
TCATC 860890 0.81730986 10.672036 110-119
GTCTA 898950 0.81711316 9.758487 30-34
GCTTG 1077770 0.81663996 16.91643 90-99
GGTGC 1385160 0.81333345 11.591037 60-69
ACAAA 862475 0.8127331 12.644978 130-139
TTATG 716940 0.80514276 11.410977 50-59
ACATC 906125 0.7997117 5.610204 110-119
TGATG 910095 0.79202884 12.15262 120-129
CCTCC 1169915 0.78270876 10.4096365 140-149
TATAC 707625 0.7715995 8.979277 130-139
GTTCA 840610 0.76408416 13.889868 160-169
CTGAC 1035275 0.7616562 9.648245 220-229
CCAGC 1265865 0.7537845 6.2970905 80-89
GGTTA 851115 0.74070024 8.057572 50-59
CGCCG 1489995 0.73960954 10.8494835 220-229
GGGAT 1095210 0.73861116 13.22063 100-109
ATAAA 627175 0.7301861 8.030556 130-139
ATCTT 621515 0.72901106 12.80435 3
GCGGA 1333795 0.7280548 8.889407 220-229
TCAAC 823715 0.72697985 7.508754 110-119
TTGGA 834370 0.7261276 6.4091754 60-69
TTTAC 615845 0.7223603 13.644978 45-49
CCCCC 1331635 0.7210869 13.234534 80-89
ACGCC 1205275 0.71770495 12.998153 180-189
ATGCC 975210 0.7174661 8.217708 50-59
GATTA 687170 0.7173987 5.6625123 50-59
AACGC 1044760 0.7145392 14.375691 180-189
GACCG 1247465 0.71120656 9.096981 210-219
TGCCA 963575 0.70890623 7.5242863 60-69
CATCC 922405 0.70878947 6.6677866 110-119
TATTG 629795 0.7072765 6.4114327 240-247
AAATA 606425 0.706028 12.678289 130-139
GACCA 1025555 0.7014044 6.0900836 170-179
AATAC 691045 0.7004891 11.40479 130-139
GGGCA 1274665 0.6957786 13.39522 150-159
CAACG 1016635 0.69530374 14.648388 180-189
CACAA 846150 0.69422305 8.174059 130-139
TCCGG 1129975 0.6929948 11.403895 70-79
TTTCT 547585 0.6909198 9.5045185 4
GGATA 848410 0.6863828 15.263337 35-39
CAAAC 832780 0.68325365 13.684087 130-139
GAAAT 702175 0.68147206 7.049586 110-119
TATAG 647720 0.67621326 6.1793795 120-129
ATTAA 536725 0.67218727 6.5451994 110-119
CACGC 1122835 0.6686144 12.61383 190-199
CCCGT 1043530 0.6684338 10.198982 140-149
CAGTC 905845 0.66643393 5.454742 210-219
AGGCA 1017185 0.6660658 6.727393 80-89
GCCGC 1341555 0.6659263 12.021976 220-229
AATAA 569535 0.66307896 7.519589 130-139
GCGAC 1136935 0.64819103 7.1456556 70-79
GACGA 986890 0.6462282 9.76024 220-229
AGATG 792390 0.64106137 5.510789 120-129
CCGTA 870510 0.6404379 6.3426666 230-239
ACTTC 665255 0.6315783 29.081867 4
TACTT 537880 0.6309107 11.542037 1
CACCG 1040460 0.61956257 8.975222 45-49
TCAAT 566735 0.6179721 8.167401 110-119
TCCAC 800370 0.615016 10.367302 180-189
AAGCA 775455 0.60913825 5.867605 120-129
CCGGC 1223035 0.6070949 9.285168 70-79
AGTAA 625420 0.6069802 5.1945844 50-59
TAGCT 666255 0.6056018 12.490742 50-59
CACGA 883515 0.60425943 8.811227 180-189
GGTGA 887485 0.59852105 6.5231805 120-129
CCCTA 777170 0.5971887 11.679538 110-119
GAAGA 792700 0.59617776 6.486108 15-19
GGCCC 1200130 0.59572524 10.357596 70-79
TGATT 527680 0.5925987 6.0719175 50-59
AATGG 732090 0.5922773 6.723952 120-129
ACGAC 855165 0.5848701 9.111321 220-229
ACCCG 976030 0.5811964 8.701686 140-149
CTTTG 594315 0.58110815 7.272638 45-49
TAATA 457935 0.5735118 8.187076 130-139
ACCTA 649695 0.57339627 7.027944 130-139
ATTTC 488415 0.5728903 6.4602966 240-247
GGGGA 1095325 0.5724343 9.564895 4
GTCCT 715395 0.56616473 6.309884 45-49
CGGTT 740320 0.5609498 11.3754635 160-169
GTTCC 707895 0.56022924 6.620681 140-149
TTAGC 615835 0.5597719 9.524097 45-49
TCAGG 787980 0.5550423 5.4256043 150-159
TTCAA 504100 0.5496744 5.214065 200-209
AAGAT 560075 0.54356176 5.926188 15-19
CTGCG 880280 0.539861 11.848937 25-29
CCACG 905110 0.53896576 7.7860975 180-189
CCTAT 566870 0.53817374 5.052713 110-119
CGGCA 941090 0.5365357 10.600959 70-79
GCTAC 721865 0.53107905 7.8613796 50-59
CTGTT 540985 0.5289633 5.762938 240-247
AAATC 521035 0.5281557 7.644417 110-119
GGAGG 1009145 0.5273953 5.306362 6
TATGT 460125 0.51673263 6.4142466 140-149
ACATT 467660 0.5099399 6.0958934 110-119
ACCCC 813450 0.5059214 8.832289 140-149
AAGTA 520485 0.50513905 5.003518 120-129
GGGTT 696180 0.50504917 5.958167 100-109
TGACG 705945 0.49725795 8.7182045 220-229
GTAAC 577320 0.4878312 6.8053236 120-129
CGCGG 1025255 0.487256 6.406111 220-229
TATCC 506495 0.48085502 5.5039606 110-119
TGGCC 774420 0.4749388 15.088717 2
TAAAT 376270 0.4712356 8.032264 130-139
CCGGT 758315 0.46506193 8.836375 160-169
AACAT 455685 0.46191263 5.529056 110-119
ATGCT 504840 0.45888138 7.0676975 50-59
CAATC 515215 0.45470932 6.18184 110-119
CATAA 444630 0.45070654 5.741606 110-119
TGCTA 493565 0.4486328 6.239196 50-59
GGTTT 478955 0.44837627 5.375468 100-109
CCGCG 903055 0.448262 6.6644626 220-229
ATTCT 379520 0.445161 7.92674 2
TGCGC 715160 0.43859565 8.7486925 25-29
TTGGT 462560 0.43302807 5.1922946 120-129
GGTCA 606095 0.42692494 5.443529 160-169
GCATG 599310 0.4221457 8.378286 60-69
GCCTT 530155 0.41956547 8.364256 1
GCGCT 678850 0.41632733 7.94465 25-29
CACTT 435095 0.4130695 6.830156 3
TACCT 433705 0.41174984 5.2466307 2
ATGGC 575750 0.4055504 7.2018404 1
ACGAG 618065 0.40471685 7.6887007 180-189
CAATG 474980 0.40135464 5.0189414 120-129
CTAAC 452240 0.39913 5.3067846 110-119
CATGC 533615 0.3925828 7.0470777 60-69
AGCTT 430610 0.39140895 6.715527 1
CCGGG 819935 0.389677 5.303788 80-89
TTTGA 345145 0.387607 6.068164 45-49
CCTAA 436320 0.38507956 7.3984594 110-119
GGTAA 468175 0.37876406 7.147489 120-129
GAGGC 688075 0.3755872 5.803823 35-39
TTCTT 282840 0.35687566 18.717276 1
TGGGC 591685 0.34742358 9.413902 1
CTCGG 565895 0.34705395 5.23697 6
CGAGC 598290 0.34109798 6.5570617 180-189
TGCTG 448320 0.33969778 13.690506 1
GCTTT 316650 0.3096134 5.1222014 45-49
CTCTT 300285 0.3066665 9.220412 1
GCGAG 557780 0.3044654 5.349223 240-247
CGCTT 384295 0.30413166 6.056167 1
TCACA 339550 0.29967403 5.2386518 7
AACTT 273435 0.29815555 10.3905525 3
CGGGA 539100 0.29426888 5.018004 80-89
GACTT 322200 0.29286817 6.810966 1
ATTGT 254635 0.28596187 5.309506 240-247
TTCCT 273555 0.27936846 6.505736 4
GAGGT 404185 0.27258295 6.6948237 7
TGTAA 256555 0.2678409 8.152205 25-29
TAACT 244280 0.26636472 7.1816525 2
TCGGT 350115 0.2652866 6.1168933 7
CCCTT 319575 0.26415697 5.719065 3
GTCTT 266935 0.26100323 12.279843 1
TTGGC 341985 0.2591264 6.8859677 1
AATCT 231300 0.2522112 7.4429502 2
CGGGG 549965 0.25024647 9.529051 3
CCTCG 383925 0.24592341 5.665491 5
TGCTT 218790 0.21392809 10.475369 1
TCGCA 249100 0.18326393 6.573588 7
TAGGC 220860 0.15557075 6.869461 1
TTGCT 127295 0.12446627 5.455782 4
Produced by FastQC (version 0.10.1)