FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_8_SL248354.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_8_SL248354.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10599471
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12198470 8.66374 61.0203 110-119
ATACA 11202050 7.523865 37.0782 110-119
CACAG 16233635 7.428056 41.86146 110-119
TCCCT 18821435 6.910484 103.109955 30-34
CAGTA 13547140 6.5548625 38.91825 110-119
AGTAA 10556700 6.364421 47.290108 110-119
GGAGA 16855230 6.213961 95.58411 35-39
GGTGA 18816915 5.8874073 102.54223 45-49
GTAAT 11455175 5.8610344 39.397457 110-119
CCCTG 18544655 5.779698 67.82631 30-34
AATAC 8072940 5.422196 39.12484 110-119
AGACG 13068830 5.3676395 102.70347 40-44
GTAGT 14417910 5.3143153 30.570175 80-89
ACAGT 10774815 5.2134566 35.423943 110-119
GTGAC 14378170 5.0117803 85.33202 45-49
GAGAC 11646110 4.7832985 142.31183 40-44
CCCCA 11670685 4.7747836 101.676 60-69
TAGTA 9221475 4.718163 39.284378 80-89
CTCAG 11926675 4.631488 34.27313 140-149
ACTCC 10491285 4.5388165 116.50629 30-34
GGCCC 16989340 4.4946256 58.954563 60-69
ACCAG 9661895 4.421012 91.77623 45-49
GCACA 9493020 4.3437395 33.96932 110-119
GTGTC 14581795 4.3136134 23.691309 120-129
TGACC 11043395 4.2884836 145.86705 45-49
CGTGT 14487570 4.2857394 23.241343 120-129
AGTAG 9838795 4.27312 31.885597 80-89
CACTC 9686855 4.1907983 95.47665 25-29
CTCCC 11728490 4.072314 67.07348 30-34
CCCAG 10873495 3.9931326 87.06005 60-69
GACCA 8552525 3.9133954 105.13272 45-49
ATGCA 8050955 3.8955011 93.51556 25-29
TGAGG 12059675 3.7732124 69.710594 35-39
CCCTT 10273215 3.7719169 39.480545 20-24
AGGAG 10121430 3.7314334 95.88619 35-39
ATGGT 10027525 3.6960578 25.54054 190-199
GACGG 12474350 3.6909401 109.39204 40-44
TACAC 6820120 3.676383 26.808409 110-119
ACGGT 10541810 3.6745453 97.064606 40-44
AGATA 6083750 3.6677701 19.864668 150-159
CCACT 8410315 3.6385317 26.904987 240-247
CTGAG 10419345 3.6318579 104.97992 35-39
TGTCC 10912035 3.5962408 20.543205 120-129
CAGGG 12090920 3.57749 34.763294 50-59
GAGGA 9559990 3.5244496 126.138435 35-39
TGGTG 13087395 3.4751272 18.019415 25-29
GCCCC 11750520 3.4632747 73.26128 60-69
TGCAC 8865335 3.4426773 112.36663 25-29
CTTGG 11491750 3.3995106 22.646479 20-24
AGGCT 9737465 3.3941758 28.467897 30-34
CCGTG 12010750 3.3600347 23.327599 120-129
GGGTT 12548950 3.3321524 41.018963 50-59
GGATG 10496150 3.2840195 68.4646 20-24
TCTTG 9408490 3.2788346 17.86029 170-179
CCTGA 8440290 3.2776198 82.27421 30-34
CTCTC 8899155 3.2674165 13.7505665 130-139
CAGAT 6732830 3.2577186 18.083096 150-159
TCAGG 9272360 3.2320547 20.772043 140-149
CCAGG 9785505 3.2256339 39.398544 50-59
CTTCA 7029465 3.2158277 16.924212 200-209
GCACT 8175585 3.1748269 86.94924 25-29
TCGCA 8063405 3.1312637 23.86858 100-109
TCTCA 6816360 3.1183367 21.728874 130-139
CCTGG 11130200 3.1136992 28.86804 50-59
TGTCT 8905285 3.1034694 19.380877 180-189
CGCAC 8322255 3.056227 24.426104 100-109
ACAGA 5331455 3.039624 14.1206875 140-149
GCCCT 9475205 2.9530787 21.188305 20-24
GGTTG 11115575 2.951545 71.75727 15-19
CGGAT 8465760 2.9508991 77.516525 20-24
TTCCC 7905970 2.9027588 48.1458 50-59
TGGCC 10373490 2.9020078 43.072758 60-69
ACCAC 5633385 2.8717165 18.999428 230-239
TCTCT 7358830 2.8570714 15.141123 180-189
GATGG 9129175 2.856322 21.972242 15-19
GATGC 8149250 2.8405738 93.5267 25-29
GGTTC 9570085 2.8310401 46.196583 50-59
AGGGT 9024840 2.8236783 48.770172 50-59
GACTC 7208375 2.79923 18.61998 190-199
CGGTG 11053220 2.7755544 68.596695 40-44
TCAAA 4085035 2.7437165 56.57211 70-79
GTTGG 10319360 2.7401242 51.75734 15-19
CGGCC 10329185 2.732644 12.483173 120-129
GCCGT 9760245 2.730451 22.041636 120-129
TTCTT 6554145 2.6908221 20.00289 170-179
TCACA 4989935 2.6898222 11.317864 140-149
AGATG 6173980 2.6814423 17.376879 180-189
TCCAT 5735800 2.624004 19.473106 70-79
AGTCA 5401470 2.6135325 30.316376 70-79
TGGAG 8330165 2.6063292 19.295776 25-29
GTTCC 7887265 2.5993779 52.160034 50-59
GGCTG 10308870 2.588642 14.812321 30-34
CCAGT 6657940 2.5854795 34.931026 60-69
TGTTC 7223635 2.5174186 13.14887 140-149
TCCTC 6808865 2.499945 16.917591 130-139
AGGGA 6735505 2.483156 8.871393 150-159
ACAGC 5369470 2.4569185 11.337309 120-129
GAGAT 5643075 2.450863 16.919306 180-189
GCGGA 8274585 2.4483037 85.32271 20-24
AATAA 2898320 2.4255185 10.48539 110-119
ACTCT 5209600 2.383279 13.144409 200-209
GGCGG 11163135 2.3794563 41.38392 20-24
CTGGA 6784955 2.3650231 14.802381 180-189
GTTCT 6693055 2.3325129 17.305119 160-169
CACGG 7075720 2.332397 9.445615 120-129
TACCA 4317395 2.32729 15.694967 230-239
CATTT 4764400 2.304811 17.900534 150-159
TTGGG 8658695 2.2991638 77.76638 15-19
TGTAG 6203325 2.2864914 7.6976347 210-219
TCATT 4720765 2.2837021 16.550398 150-159
CTGGC 8150870 2.2802246 33.86381 60-69
CAGCT 5830460 2.264144 11.735462 150-159
GTGAA 5212375 2.263804 18.176832 190-199
TTCAT 4671020 2.2596378 16.203424 140-149
TTCAC 4865065 2.2256618 18.692999 200-209
CAGAG 5400325 2.2180252 9.110625 140-149
GATAC 4551315 2.2021801 14.828243 150-159
TTGTC 6295875 2.1940966 13.358392 170-179
ACTAC 4052230 2.1843529 12.455163 230-239
GAATC 4500855 2.177765 14.822853 190-199
ATTTG 4962755 2.15495 15.74433 150-159
CTACT 4663725 2.133553 7.6371307 230-239
TGAAT 4157785 2.1273286 14.288553 190-199
CACTA 3936730 2.1220927 14.764462 240-247
TCAGC 5445150 2.1145163 13.848355 140-149
CTCTG 6319915 2.0828319 14.53456 180-189
TACAG 4294980 2.078151 13.817806 160-169
CTCGC 6667285 2.0779514 16.29903 100-109
TCTGC 6302985 2.0772521 11.926087 210-219
ATGTA 4050555 2.0724645 8.680704 70-79
GCTGT 6980670 2.0650346 10.24798 140-149
GTCTC 6248955 2.0594459 11.916945 180-189
GTCCT 6231740 2.0537724 14.33063 130-139
TTGGA 5562280 2.0502079 15.433766 170-179
TGGGG 9076535 2.0458238 51.484863 15-19
ATATA 2877620 2.043777 10.676297 110-119
ACACA 3213025 2.0408025 13.887776 110-119
CAGGC 6177930 2.036455 15.341418 140-149
GTTCA 4942790 2.029691 15.194981 140-149
TCTCG 6105215 2.0120738 15.79018 100-109
TAGTC 4894640 2.009919 24.930193 70-79
AATCG 4151315 2.008638 13.456811 190-199
TTGCA 4860355 1.9958402 14.26168 150-159
ATCTC 4347545 1.9889076 7.980463 100-109
AATAT 2795365 1.9853568 13.32191 110-119
GAACT 4092675 1.9802645 10.510403 160-169
TTTGC 5675295 1.977826 12.115249 150-159
AGCTC 5074285 1.9704983 11.898046 140-149
TGGAA 4521180 1.9636087 9.128677 170-179
ACGGC 5935275 1.9564675 14.279909 120-129
CCTTC 5324060 1.9547838 13.933221 200-209
GAGTC 5598530 1.9514726 12.255662 210-219
GATAT 3793265 1.9408221 7.906847 180-189
AAAAA 1963305 1.9359951 7.971141 8
GTCAA 3993295 1.9321789 38.97442 70-79
AACCA 3024470 1.921039 14.47469 70-79
TCGGC 6863995 1.9202183 7.850273 130-139
CCAGA 4181520 1.9133462 29.578247 60-69
ACACG 4158395 1.9027647 13.107028 120-129
CAGCC 5168070 1.8978984 10.340649 120-129
TACTA 3328775 1.8974475 10.192542 230-239
ATATG 3697060 1.8915989 8.148318 180-189
CATAG 3866965 1.8710535 8.2205515 210-219
GACGT 5298020 1.846724 9.367301 170-179
AGTAT 3579105 1.8312473 11.938264 220-229
GGGGC 8544200 1.8212223 46.94492 15-19
CTGTT 5225235 1.820981 12.822108 140-149
CCTTG 5521575 1.8197259 25.681162 20-24
GTTGT 5734255 1.7937579 6.7895494 210-219
CGTAG 5132040 1.7888687 9.742738 210-219
GGGCG 8327590 1.7750512 57.7041 20-24
GCAGA 4298865 1.7656329 14.613862 150-159
ATTGT 4003015 1.7382072 8.100951 50-59
TGCAG 4971300 1.7328397 13.544736 150-159
AGAAC 3029145 1.7270075 11.158509 160-169
AGTCT 4194810 1.7225429 12.903239 210-219
CACCA 3356115 1.7108383 13.148351 230-239
GTCCC 5407230 1.6852379 22.175545 50-59
ATAGT 3282055 1.6792618 7.2268205 220-229
GGTCA 4800785 1.6734037 7.398708 130-139
CTCCA 3867415 1.6731492 7.4705596 150-159
CAAAA 2106490 1.6670976 21.855923 70-79
GTCAC 4269920 1.6581389 8.217123 130-139
TCCCA 3825550 1.6550373 10.596056 240-247
TCAGA 3405925 1.6479765 10.029814 130-139
TGACT 4010430 1.6468297 11.215923 190-199
TTGGT 5249870 1.6422353 27.057396 25-29
AATAG 2697440 1.626232 9.33217 240-247
TCTGG 5479295 1.620895 11.965434 180-189
AACTG 3342370 1.6172253 10.387733 160-169
TGGTT 5127530 1.6039655 17.555346 10-14
GAGAA 3125565 1.5995207 10.33417 160-169
CTGTG 5368030 1.5879805 5.166369 210-219
GTCTG 5361155 1.5859468 8.929378 210-219
ATAGA 2630540 1.5858994 9.923533 240-247
TGTGC 5320965 1.5740576 7.9217906 220-229
TTCAG 3829705 1.5726175 9.131834 150-159
GCTCC 5024305 1.565894 6.068868 150-159
CAGAC 3415765 1.5629581 17.822609 60-69
GAACC 3414080 1.5621872 11.9985895 70-79
ACCAT 2882275 1.5536891 12.48236 45-49
ACTGA 3205830 1.5511595 8.355323 180-189
GGCCG 6513295 1.5466964 12.906643 120-129
TGCTC 4691795 1.5462582 5.224505 150-159
TTGTA 3555700 1.5439723 8.5413475 210-219
CTGGT 5201445 1.5387008 6.7585444 160-169
GTGCT 5192710 1.536117 7.7234755 220-229
TAGTT 3528545 1.5321809 9.276705 220-229
GCTGA 4385715 1.5287232 24.230894 30-34
GGACG 5147775 1.5231358 6.993435 170-179
CCACC 3720660 1.5222195 9.482839 230-239
CCCAC 3718370 1.5212827 10.393728 240-247
CAAAG 2650695 1.5112416 27.80736 70-79
GCTCA 3877065 1.5055814 10.677343 140-149
CCTCG 4805975 1.4978486 9.672889 130-139
GCTCT 4477090 1.4754987 9.969362 130-139
CATGT 3543090 1.4549229 8.649665 70-79
CGACT 3655845 1.4196751 8.259457 190-199
CACTG 3609530 1.4016896 7.0259666 230-239
GTGGA 4473110 1.3995397 24.35169 25-29
ACCGT 3590510 1.3943036 21.03395 45-49
AATTG 2721950 1.3926843 9.196845 170-179
TCGAC 3584520 1.3919773 8.983898 190-199
TATAC 2437605 1.3894683 6.782949 110-119
GCGTA 3949385 1.3766322 10.302931 210-219
TGATA 2688570 1.3756055 7.3723483 180-189
CTCTT 3539710 1.3742951 7.8071375 200-209
CCTCA 3175790 1.3739336 7.27962 130-139
TGGAC 3941425 1.3738575 9.971385 170-179
ACGGA 3308200 1.3587463 9.501847 200-209
CCATC 3132185 1.3550687 10.692964 240-247
GGGAG 5060215 1.3439257 6.3662686 150-159
GAGGG 5032195 1.3364842 7.8921685 200-209
GTCCA 3431880 1.3327026 18.278362 70-79
CTTGA 3240595 1.3307072 8.077073 200-209
GTAGG 4243550 1.3277154 5.0151873 160-169
CCATG 3415235 1.3262389 8.790808 70-79
ACTTC 2895480 1.3246193 6.7027225 220-229
GGAGG 4946140 1.3136292 18.488937 30-34
CTGCT 3961780 1.3056697 5.4747434 90-99
GAATT 2547935 1.3036498 10.137134 170-179
CTCGA 3351655 1.3015488 7.981675 190-199
TATGG 3520550 1.2976439 5.9989743 180-189
GTGTT 4144480 1.2964532 7.70535 160-169
TCCAC 2986110 1.2918726 9.163547 240-247
AGATC 2666540 1.290221 6.754121 130-139
AGCCG 3914020 1.2901936 9.235947 120-129
CTGAT 3108785 1.2765812 7.0303144 180-189
GGAGT 4078445 1.2760577 7.0133214 210-219
ATCGG 3653475 1.2734873 8.0704 190-199
TTGGC 4285180 1.2676498 8.8413515 60-69
GTGGT 4769925 1.2665694 11.998501 50-59
TGGTC 4263265 1.2611668 13.532681 50-59
GGCTC 4476405 1.2522846 8.333116 130-139
ATAAA 1494470 1.2506778 5.5793943 7
ACGTG 3562720 1.2418529 9.013822 170-179
AAGTA 2045005 1.2328923 15.524664 70-79
ACTAT 2154515 1.228103 6.130716 230-239
ATAAC 1824860 1.2256685 7.252951 80-89
GGAAT 2813740 1.2220448 8.473285 170-179
TGCCC 3891425 1.2128164 7.632643 60-69
GATCT 2933780 1.2047178 5.3988566 130-139
ACTCG 3094485 1.2016821 8.231789 190-199
GCATA 2459185 1.189891 7.885726 210-219
AAAGT 1963055 1.1834863 20.295286 70-79
TTGAG 3203195 1.1806698 7.772884 200-209
ATACC 2181755 1.1760741 8.234188 70-79
TTCCA 2563295 1.172652 7.3401384 230-239
ACTGG 3357970 1.1704835 6.933795 160-169
TCACG 2998385 1.1643634 9.643626 200-209
CCATA 2157765 1.1631422 11.314219 70-79
ACTGT 2811125 1.1543511 5.3543897 230-239
CAATA 1715280 1.1520691 6.695334 100-109
TATCA 2013370 1.1476485 7.125576 70-79
AAAAG 1598035 1.1352074 14.903406 70-79
AGCTT 2754470 1.1310866 5.9362473 150-159
ATGGG 3592435 1.1239955 34.67359 15-19
AGTTC 2731885 1.1218123 5.4814253 160-169
TCTGT 3216210 1.1208409 6.27743 210-219
AGAGC 2668725 1.0961007 6.6844163 140-149
CGGCT 3918055 1.0960848 7.8265405 130-139
CTCGG 3912975 1.0946637 8.282954 130-139
AGGTT 2961480 1.0915757 22.791447 10-14
AGAGA 2132420 1.0912747 14.136952 35-39
GAAGA 2131530 1.0908194 19.247677 35-39
TATGT 2474170 1.0743452 7.4560657 220-229
ATCCA 1981535 1.0681456 14.7663765 240-247
TCTAC 2324115 1.0632322 6.8876934 180-189
GTCTA 2583815 1.0610093 7.1007466 180-189
ATATC 1858185 1.059191 7.5494485 70-79
CTGCC 3361430 1.0476359 5.6871166 200-209
CTGCG 3726750 1.0425668 6.849608 210-219
CTGAA 2151670 1.0410979 15.455226 35-39
AACTT 1818980 1.0368437 5.820451 200-209
TACTG 2516425 1.0333365 6.0454483 180-189
GCTTC 3132445 1.0323488 5.259913 150-159
GAGCT 2958195 1.0311342 5.9794135 140-149
CTACC 2368980 1.0248854 8.199213 230-239
CATGG 2922455 1.0186765 5.236265 190-199
AAAAT 1210805 1.0132871 5.0529976 8
TCCCC 2912850 1.0113868 5.278042 80-89
GCGGT 4014845 1.0081606 5.6626086 130-139
ATACG 2073670 1.0033573 5.1566334 120-129
TAGAT 1960700 1.0031912 7.600489 240-247
TGCGT 3375845 0.99864864 8.177908 210-219
TATAA 1398980 0.99359995 6.479289 240-247
GGGGT 4357560 0.982181 7.493818 10-14
CTTCT 2514050 0.9760818 5.194762 210-219
CGTGG 3886430 0.9759145 8.282982 50-59
ACAAT 1447430 0.9721673 7.651347 100-109
CGGAG 3280355 0.9705992 6.5958457 210-219
CAGCG 2909575 0.9590945 6.2204976 160-169
CCATT 2094100 0.95800537 6.1052775 50-59
CGAAC 2092785 0.9575996 12.565761 70-79
ACCCA 1862890 0.9496408 12.546626 240-247
GAGTT 2553315 0.9411296 5.274756 160-169
TAGGC 2696325 0.93985456 5.610259 160-169
AAGAG 1832965 0.9380275 14.280445 35-39
ACGTC 2405950 0.93430316 12.503277 70-79
GAGGC 3154255 0.9332884 15.710086 30-34
TGAAG 2145200 0.931689 10.3691845 35-39
GGTGG 4119475 0.92851734 24.257105 25-29
CCCAA 1816325 0.9259034 9.169731 240-247
ACTAA 1375575 0.92390597 12.088853 240-247
TGTAA 1792450 0.91710615 6.46598 230-239
TGCAT 2209145 0.907156 5.989127 210-219
CATCA 1666080 0.8980997 5.7152066 230-239
TCGAA 1821990 0.8815803 16.269293 70-79
ATCAA 1311185 0.8806584 13.618779 70-79
GGGCC 3704480 0.87969387 18.369871 15-19
TTCTG 2489615 0.8676245 5.2031918 210-219
CCAAT 1586315 0.85510236 10.070014 240-247
CGTCC 2712145 0.8452774 10.226964 70-79
TGAAC 1737560 0.8407285 6.345849 190-199
AGCGT 2410550 0.8402423 6.3900547 160-169
CGGTC 2968510 0.83044755 5.3497386 130-139
TGGGC 3307105 0.8304412 19.906435 15-19
CCAGC 2257300 0.82896054 5.619006 80-89
GACCG 2502345 0.8248577 19.457901 45-49
GTATG 2230985 0.8223216 5.232039 220-229
CTTCC 2219470 0.8149013 5.1327634 230-239
GGTCC 2912140 0.8146779 13.758579 50-59
AACCG 1728700 0.7910046 5.715858 190-199
TAACC 1455300 0.7844789 5.823561 80-89
TGAGA 1791205 0.7779442 5.7295065 240-247
AAAGA 1082600 0.7690542 5.694979 8
CATAC 1392340 0.7505403 9.799632 70-79
AAAGG 1452495 0.7433204 8.721095 10-14
CTAAT 1297130 0.73938185 9.38167 240-247
AGCCA 1604440 0.7341467 5.889842 80-89
AATCC 1349955 0.7276927 8.937449 240-247
CCTTT 1856425 0.7207585 5.135033 50-59
GTCGA 2050965 0.71490234 11.478943 70-79
ATGAT 1392320 0.7123798 6.5133286 240-247
GTAGC 2038745 0.71064276 5.497852 80-89
AAAGC 1222860 0.6971896 10.10988 70-79
AAGGT 1568330 0.68114674 9.667094 10-14
CATTG 1653785 0.67910486 5.5943117 50-59
AGGAT 1544730 0.6708969 6.65251 10-14
CGATG 1896780 0.66115826 15.098841 15-19
GGAAG 1781460 0.6567648 6.5418715 30-34
TAGGT 1777685 0.6552391 5.3348784 10-14
AGAAG 1256425 0.64298075 5.8317757 9
GAGGT 2039985 0.6382674 6.7398586 10-14
CCGTA 1638515 0.6362848 5.3756084 80-89
TATGA 1238675 0.63376737 5.4869075 240-247
CAATC 1160715 0.62568295 8.62098 240-247
ATAGG 1410395 0.6125534 5.1895285 10-14
TAAAA 697840 0.5840017 5.728454 8
CATCC 1342995 0.5810163 7.204624 240-247
AATGG 1325930 0.57586914 6.476021 10-14
AAATG 942710 0.5683408 5.3198338 9
AAGAA 795480 0.5650907 5.3815184 8
GGGTC 2222175 0.55800635 5.482932 70-79
GAAAA 775710 0.5510466 6.071659 8
GAAGG 1465585 0.5403123 5.5992584 10-14
AAGCA 933940 0.53246754 6.8236036 70-79
AAGGG 1391430 0.51297385 6.274645 10-14
ACGAT 1027365 0.49709648 6.8658466 10-14
TGATC 1197355 0.49167794 5.118649 240-247
TTGCC 1480960 0.48807475 5.345188 60-69
ATAAG 797440 0.48076046 5.2952604 9
GGTCG 1910095 0.47964054 5.554653 70-79
GATCC 1221530 0.47435698 5.1504683 240-247
CGGTT 1496105 0.44258052 7.929771 10-14
GAAGC 1075000 0.4415248 5.288028 30-34
TAAGG 998975 0.4338682 5.847098 10-14
TACCG 1115655 0.43324256 5.1878166 70-79
AAGCT 867605 0.41979572 8.875226 30-34
TAAAG 669195 0.40344414 5.506743 9
Produced by FastQC (version 0.10.1)