FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_17_SL248346.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_17_SL248346.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7015073
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7989305 8.322047 58.646687 110-119
CACAG 10597630 7.2355666 40.995266 110-119
ATACA 7309115 7.2134323 35.37633 110-119
TCCCT 12446270 7.0543957 106.22066 30-34
CAGTA 8975870 6.468215 38.284348 110-119
AGTAA 6931800 6.1186285 45.80285 110-119
GGAGA 11036610 6.027834 91.91613 35-39
CCCTG 12455115 5.982122 69.76263 30-34
GGTGA 12298050 5.787696 100.55599 45-49
GTAAT 7547245 5.740377 38.508755 110-119
AATAC 5299400 5.2300253 37.477257 110-119
GTAGT 9344405 5.1896048 27.75722 80-89
AGACG 8377435 5.1157084 98.881165 40-44
ACAGT 6988665 5.0361896 34.68583 110-119
GTGAC 9430025 4.961939 82.95249 45-49
GAGAC 7733005 4.722185 138.51457 40-44
CCCCA 7552340 4.7066755 100.589584 60-69
ACTCC 7107905 4.675396 119.7012 30-34
CTCAG 7812110 4.5959578 34.23322 140-149
ACCAG 6616155 4.5172014 91.71313 45-49
GGCCC 10875120 4.4261727 59.643734 60-69
TAGTA 5803980 4.414463 34.856556 80-89
GCACA 6336900 4.3265395 33.33979 110-119
GTGTC 9523765 4.3180847 23.543633 120-129
CGTGT 9457985 4.28826 23.049103 120-129
CACTC 6469465 4.2554464 97.073654 25-29
TGACC 7184970 4.227004 141.60983 45-49
CTCCC 7740480 4.1566596 69.16211 30-34
AGTAG 6336635 4.0841103 28.232382 80-89
CCCAG 7056175 3.9330819 85.81236 60-69
GACCA 5759580 3.932372 103.59496 45-49
ATGCA 5444505 3.9234333 92.94114 25-29
AAAAA 2714810 3.8086655 25.165857 130-139
TGAGG 7962425 3.7472687 68.09224 35-39
AGGAG 6672445 3.6442704 92.20642 35-39
CCACT 5533500 3.639793 26.725225 240-247
CAGGG 8153665 3.6356184 35.519947 50-59
ATGGT 6527745 3.6253157 25.192028 190-199
GACGG 8124405 3.6225715 106.78086 40-44
ACGGT 6879135 3.619699 94.91982 40-44
TACAC 4473330 3.6041918 26.008854 110-119
CTGAG 6843105 3.60074 104.29343 35-39
TGTCC 7046335 3.572028 20.40406 120-129
CCCTT 6295085 3.5679786 35.214516 20-24
TGCAC 5950260 3.5006092 113.66696 25-29
AGATA 3940660 3.47838 18.76096 150-159
GGGTT 8517230 3.453916 42.611324 50-59
GCCCC 7578610 3.4486809 73.43982 60-69
GAGGA 6272455 3.425809 120.85141 35-39
TGGTG 8385435 3.4004705 17.875885 190-199
CCTGA 5679255 3.341174 83.23485 30-34
CCGTG 7662030 3.291412 23.389044 120-129
GGATG 6990695 3.2899542 68.97771 20-24
CCAGG 6589080 3.2848785 40.294476 50-59
TCTTG 6122535 3.2758718 17.859241 170-179
CTTCA 4714185 3.27286 16.798151 200-209
CTTGG 7207410 3.2678468 19.984705 20-24
CTCTC 5742775 3.2549357 13.6578455 130-139
AGGCT 6181250 3.2524817 24.322357 30-34
GCACT 5507150 3.2399223 88.06149 25-29
CCTGG 7415950 3.1857028 29.770235 50-59
TCAGG 6037045 3.176603 20.626114 140-149
CAGAT 4403245 3.1730778 17.562725 150-159
TGTCT 5807315 3.1072128 19.599878 180-189
TCGCA 5279530 3.1060102 23.543167 100-109
TCTCA 4414590 3.0648637 21.337465 130-139
CGCAC 5437050 3.0305884 24.035452 100-109
GGTTG 7452320 3.022072 74.0893 15-19
TTCCC 5329085 3.0204613 49.95297 50-59
CGGAT 5691165 2.9946067 78.29831 20-24
GGTTC 6473610 2.9351413 48.102417 50-59
TGGCC 6825005 2.9318476 43.91088 60-69
ACAGA 3500155 2.927192 13.606888 140-149
GATGC 5506905 2.8976514 95.42189 25-29
AGGGT 6128495 2.8841863 49.67997 50-59
ACCAC 3771835 2.8792908 19.238417 230-239
GCCCT 5950285 2.8578885 19.084332 20-24
TCTCT 4740835 2.8360868 15.253114 180-189
GTTGG 6952120 2.8192306 52.699097 15-19
GACTC 4751330 2.7952642 18.784374 190-199
GCCGT 6448465 2.7700956 22.135677 120-129
CGGTG 7207465 2.7691844 68.08199 40-44
CCAGT 4658415 2.7406013 36.66088 60-69
AGTCA 3798130 2.7370183 31.317438 70-79
CGGCC 6687260 2.7217143 12.66054 120-129
GATGG 5764480 2.712874 19.390814 15-19
TCAAA 2745545 2.7096033 55.656597 70-79
TTCTT 4267330 2.6944182 20.1069 170-179
GTTCC 5274915 2.6740346 54.501423 50-59
AGATG 3989400 2.5712621 16.658525 180-189
TCACA 3173180 2.5566523 10.935052 140-149
GGCTG 6562235 2.52128 12.74216 30-34
TCCAT 3623435 2.5155985 17.109196 70-79
GAGAT 3885570 2.5043414 16.310703 180-189
TCCTC 4390675 2.4885817 17.107462 130-139
GCGGA 5579640 2.4878924 85.957794 20-24
TGTTC 4646120 2.4859135 12.984575 140-149
TGGAG 5211510 2.4526358 16.4353 25-29
AGGGA 4481250 2.4475117 8.648382 150-159
GGCGG 7487690 2.4378333 42.181236 20-24
ACAGC 3500700 2.3901143 10.903976 120-129
ACTCT 3412080 2.3688636 12.99949 200-209
TTGGG 5834505 2.3660147 79.28924 15-19
CTGGC 5479340 2.3537843 35.275 60-69
AATAA 1937370 2.342016 9.567555 110-119
TACCA 2893605 2.3313968 15.912102 230-239
GTTCT 4355765 2.3305588 17.293005 160-169
CACGG 4638685 2.3125405 9.395698 120-129
CTGGA 4373050 2.3010342 14.46993 180-189
CAGCT 3899285 2.293996 11.891471 150-159
TGTAG 4057015 2.2531457 7.6564264 210-219
CATTT 3059075 2.241584 17.601389 150-159
TCATT 3054435 2.238184 16.470037 150-159
TTCAC 3195330 2.2183833 18.551949 200-209
TCAGC 3756425 2.2099497 13.965335 140-149
TTCAT 3005390 2.2022455 15.909815 140-149
ACTAC 2711800 2.1849153 12.067754 230-239
TCTGC 4295885 2.1777308 12.230639 210-219
TTGTC 4051125 2.1675608 13.214097 170-179
GTGAA 3360055 2.1656344 17.583424 190-199
CTACT 3109130 2.158538 7.276072 230-239
CAGAG 3518890 2.1488218 8.955649 140-149
CACTA 2649595 2.1347964 14.8717985 240-247
TGGGG 6135750 2.1084723 52.5545 15-19
ATTTG 3213890 2.106332 15.471915 150-159
GATAC 2920110 2.1042974 14.221086 150-159
CTCGC 4320310 2.0750203 16.18173 100-109
TCTCG 4091870 2.0743086 16.917805 100-109
CTCTG 4089865 2.0732923 14.650439 180-189
GCTGT 4571445 2.0726976 10.167388 140-149
ATCTC 2973135 2.0641224 9.231684 100-109
GAACT 2855945 2.0580585 10.2789955 160-169
TAGTC 3312260 2.0567248 26.087378 70-79
GTCTC 4051330 2.0537574 11.960179 180-189
TTGGA 3693135 2.0510576 15.550195 170-179
GTCCT 4044425 2.0502572 14.488108 130-139
ACACA 2168910 2.0280309 13.195548 110-119
GAATC 2798060 2.0163453 14.225075 190-199
TACAG 2796815 2.0154479 13.453031 160-169
ATATA 1931035 2.0114596 10.349158 110-119
GTTCA 3224625 2.0023084 14.868222 140-149
CAGGC 3999000 1.9936363 15.3605585 140-149
AGCTC 3379745 1.9883444 11.920695 140-149
ATGTA 2605555 1.9817653 7.430528 70-79
GATAT 2597935 1.9759696 7.718328 180-189
ACACG 2878035 1.9649878 12.83592 120-129
GTCAA 2723395 1.9625398 39.61467 70-79
TGAAT 2577125 1.9601417 13.511054 190-199
TTTGC 3640985 1.9481148 12.167849 150-159
TTGCA 3135425 1.9469204 14.145072 150-159
CCTTC 3431265 1.9447998 14.076967 200-209
AACCA 2076580 1.9416981 14.863378 70-79
AATCG 2691405 1.9394871 13.065873 190-199
AATAT 1861940 1.9394869 12.985973 110-119
ACGGC 3871775 1.9302102 14.197071 120-129
TGGAA 2981280 1.9215051 9.21501 170-179
GAGTC 3628650 1.9093416 12.175855 210-219
TCGGC 4393870 1.8874942 7.937079 130-139
CGTAG 3580100 1.8837953 9.686634 210-219
GGGGC 5774495 1.8800533 47.9832 15-19
TACTA 2204020 1.8742889 9.5417185 230-239
CAGCC 3348725 1.866565 10.168377 120-129
ATATG 2425015 1.8444477 7.9677024 180-189
CATAG 2550820 1.8381784 8.061818 210-219
GGGCG 5621795 1.8303374 59.58898 20-24
CTGTT 3353870 1.7944932 12.803855 140-149
AGTAT 2342420 1.7816268 11.563723 220-229
GTTGT 3708835 1.7748597 6.81826 210-219
CCAGA 2589815 1.7682048 25.79405 60-69
GACGT 3357680 1.7667612 9.355519 170-179
CTCCA 2666825 1.7541683 7.791423 150-159
GTCAC 2939800 1.7295195 8.235767 130-139
ATTGT 2629610 1.7234042 7.509745 170-179
CACCA 2255665 1.7218981 13.22274 230-239
AGTCT 2760775 1.714284 12.992424 210-219
TGCAG 3242750 1.7062868 13.37372 150-159
GCAGA 2788115 1.7025716 14.224089 150-159
AGAAC 2033055 1.7002512 10.697985 160-169
CCTTG 3326300 1.6862152 22.734816 20-24
GTCTG 3672365 1.665054 9.08817 210-219
GGTCA 3149900 1.6574305 7.3906827 130-139
TGACT 2665645 1.6552136 11.383079 190-199
CAAAA 1442200 1.6518049 20.142883 70-79
ATAGT 2170740 1.6510481 7.253167 220-229
TTCAG 2645360 1.642618 9.04719 150-159
TGGTT 3417670 1.6355231 18.01248 10-14
TCAGA 2257870 1.627072 9.923968 130-139
CTGTG 3569310 1.6183287 5.2953434 210-219
TGTGC 3558960 1.613636 8.126794 220-229
TCCCA 2444640 1.6080208 10.4312725 240-247
AACTG 2223300 1.6021601 10.195964 160-169
TCTGG 3522595 1.5971481 12.030256 180-189
GTCCC 3317095 1.5931818 20.139132 50-59
TGCTC 3138650 1.5910889 5.4321237 150-159
TTGGT 3313385 1.5856175 24.20603 25-29
GCTCC 3300490 1.5852066 6.3828945 150-159
GTGCT 3466605 1.5717622 7.8956637 220-229
GAGAA 2096300 1.5680082 9.769077 160-169
GAACC 2291835 1.5647578 12.537241 70-79
CTGGT 3441810 1.56052 6.809842 160-169
AATAG 1765155 1.558084 8.937594 240-247
TTGTA 2370925 1.5538663 8.563871 210-219
TAGTT 2370155 1.5533615 9.5845175 220-229
GGCCG 4266225 1.5529912 13.023927 120-129
ACTGA 2147540 1.5475657 8.287884 180-189
CAAAG 1838050 1.5371679 28.065872 70-79
ATAGA 1730385 1.527393 9.44581 240-247
GCTCA 2577720 1.5165032 10.764326 140-149
GGACG 3397570 1.5149343 7.0195565 170-179
AGATC 2096620 1.5108718 6.817873 130-139
CCACC 2412065 1.5032171 9.663629 230-239
ACCAT 1846375 1.4876367 11.165794 45-49
GCTCT 2920200 1.4803491 10.044478 130-139
CCCAC 2367010 1.4751387 10.023108 240-247
CCTCG 3060845 1.4701067 9.695581 130-139
CAGAC 2127245 1.4523834 15.234499 60-69
GCTGA 2690610 1.4157592 20.741877 30-34
CATGT 2279890 1.4156816 7.5674415 70-79
CGACT 2397575 1.4105221 8.280722 190-199
CTGCT 2762440 1.4003751 5.783703 90-99
TATAC 1642270 1.3965791 6.6461287 110-119
CACTG 2368650 1.3935051 6.9237027 230-239
AATTG 1821470 1.385396 9.158149 170-179
CTCTT 2315175 1.3849959 7.876785 200-209
GCGTA 2629130 1.3834093 10.100811 210-219
TCGAC 2343355 1.3786238 8.947739 190-199
TGGAC 2605775 1.37112 10.014029 170-179
CCTCA 2065575 1.3586818 7.4178386 130-139
GAGGG 3398935 1.3554989 7.8457932 200-209
TGATA 1781015 1.3546264 7.252546 180-189
CTTGA 2172555 1.3490329 7.980526 200-209
GATCT 2157875 1.3399174 5.397371 130-139
GGGAG 3359470 1.3397603 6.301639 150-159
ACGGA 2187630 1.3358835 9.230028 200-209
ACTTC 1911475 1.3270566 6.6139874 220-229
CCATC 2013190 1.3242242 10.542482 240-247
GTAGG 2780215 1.3084221 5.0898404 160-169
GTGGA 2779995 1.3083186 21.038067 25-29
GGAAT 2014100 1.2981348 8.564726 170-179
ATCGG 2460805 1.2948389 7.7414274 190-199
GAATT 1700255 1.293201 10.251374 170-179
CTCGA 2191180 1.2890972 8.009002 190-199
GTGTT 2688055 1.2863665 7.598052 160-169
CCATG 2185545 1.2857823 7.6614776 70-79
TCACG 2184725 1.2852999 9.6115885 200-209
CTGAT 2065870 1.2827874 7.030204 180-189
TCCAC 1942535 1.2777492 9.237431 240-247
TATGG 2293825 1.2739222 5.9112782 180-189
ACCGT 2156570 1.2687359 18.102772 45-49
GGAGT 2682280 1.262332 7.028986 210-219
AGCCG 2505300 1.2489763 9.027839 120-129
GGCTC 2900070 1.245796 8.377922 130-139
ACGTG 2362855 1.2432994 8.982664 170-179
ATAAA 1026360 1.2407292 5.135366 7
ACTAT 1448020 1.2313899 5.9647026 230-239
GTCCA 2091920 1.2307013 16.151552 70-79
GGAGG 3085315 1.2304269 15.987206 30-34
ATAAC 1246170 1.2298564 7.332737 80-89
AAGTA 1382520 1.2203362 15.397514 70-79
AAAGT 1367755 1.2073032 20.662495 70-79
ACTCG 2050725 1.2064661 8.229138 190-199
GTGGT 2974855 1.2063664 10.588368 50-59
AGAGA 1612720 1.206296 12.402665 35-39
TGGTC 2641960 1.1978674 12.006961 50-59
TTGGC 2621495 1.1885885 8.157708 60-69
TGCCC 2464320 1.1835989 6.788673 60-69
TTCCA 1695830 1.1773434 7.497785 230-239
TTGAG 2119520 1.1771185 7.6905518 200-209
ACTGG 2236860 1.1770024 6.9270673 160-169
GCATA 1622800 1.1694263 7.7701087 210-219
AGAGC 1905610 1.163667 6.6640606 140-149
AAAAG 1116010 1.1432264 13.418964 70-79
AGCTT 1838485 1.1415943 5.832898 150-159
ACTGT 1832145 1.1376575 5.320589 230-239
TCTGT 2124875 1.1369176 6.5227637 210-219
GAAGA 1518770 1.1360224 18.133282 35-39
ATACC 1408105 1.1345196 7.201937 70-79
TATCA 1324890 1.1266806 6.599317 70-79
ATATC 1324135 1.1260386 7.0486875 70-79
CAATA 1121785 1.1070999 6.423961 100-109
CCATA 1363795 1.0988188 10.012785 70-79
CTGAA 1515095 1.0918115 14.179532 35-39
CGGCT 2536550 1.0896369 7.8638167 130-139
AGTTC 1749355 1.0862497 5.1775856 160-169
AGGTT 1952925 1.0845965 22.640558 10-14
CTCGG 2523225 1.0839128 8.3921385 130-139
TCTAC 1559265 1.082532 6.8598213 180-189
GTCTA 1730305 1.0744208 7.0073533 180-189
CTTCT 1794225 1.0733505 5.5459757 210-219
GCTTC 2103350 1.06626 5.3023443 150-159
CTGCG 2470495 1.0612614 6.9018316 210-219
AAAAT 877740 1.0610679 5.1334114 8
CTGCC 2204455 1.0587872 5.874945 200-209
TATGT 1610580 1.0555483 7.359483 220-229
TACTG 1692605 1.0510112 6.1453805 180-189
ATCCA 1296620 1.0446954 14.346701 240-247
CTACC 1587445 1.0441803 8.430427 230-239
ATGGG 2207805 1.0390351 30.302322 15-19
GAGCT 1970480 1.0368372 6.0589814 140-149
AGGGG 2589705 1.0327772 5.0233774 60-69
GGGGT 2996095 1.0295697 7.755971 10-14
AACTT 1207135 1.0265423 5.7971044 210-219
GCGGT 2660520 1.0221999 5.7142477 130-139
TGCGT 2230965 1.0115218 8.182293 210-219
CATGG 1920035 1.0102938 5.223527 190-199
TAGAT 1312170 0.9980265 7.2787766 240-247
ATACG 1366385 0.9846478 5.180558 120-129
TATAA 944735 0.98408175 6.262427 240-247
AAGAG 1314680 0.9833654 13.42335 35-39
CAGCG 1967005 0.98061836 6.305082 160-169
TCCCC 1819965 0.97732633 5.0721517 80-89
CGAAC 1412625 0.9644743 13.12964 70-79
CGGAG 2161685 0.9638686 6.6358757 210-219
TTCTG 1781050 0.9529536 5.4840145 210-219
AGCTG 1801980 0.948175 5.1019983 90-99
ACAAT 959990 0.94742286 7.3797307 100-109
CGTGG 2440365 0.937614 7.4057307 50-59
TAGGC 1780675 0.9369647 5.7807975 160-169
TGAAC 1297515 0.93501866 6.422677 190-199
ACGTC 1568500 0.92276734 11.094373 70-79
ACCCA 1206470 0.92097825 12.0125675 240-247
ACTAA 932430 0.92022365 11.797331 240-247
CCATT 1318135 0.9151258 5.7335796 50-59
TGTAA 1198480 0.91155463 6.4200616 230-239
TGCAT 1462220 0.9079552 5.99284 210-219
CCCAA 1179785 0.900608 9.158613 240-247
TCGAA 1237585 0.89183164 16.537245 70-79
GTATG 1593120 0.8847715 5.2136216 220-229
TGAAG 1358530 0.87560457 9.192006 35-39
CATCA 1086085 0.8750659 5.6137414 230-239
GGTGG 2534090 0.87080765 21.335493 25-29
AGCGT 1629245 0.8572845 6.466415 160-169
CCAGC 1536090 0.85621 5.67179 80-89
GAGGC 1908850 0.8511326 13.664911 30-34
CGGTC 1958665 0.8413924 5.431702 130-139
CCAAT 1038985 0.8371172 9.954341 240-247
AAAGA 817135 0.8370626 5.3065886 8
CTTCC 1462275 0.82879984 5.407904 230-239
ATCAA 839540 0.8285497 12.229058 70-79
GGGCC 2238410 0.8148259 16.225048 15-19
GACCG 1628820 0.81202155 16.857668 45-49
TAACC 989130 0.7969486 6.0159545 80-89
AACCG 1163740 0.79454726 5.6885433 190-199
CGTCC 1640810 0.7880717 9.260822 70-79
TGAGA 1206195 0.77742106 5.5145993 240-247
TGGGC 1982270 0.76160914 17.54887 15-19
GGTCC 1768035 0.7595026 12.293351 50-59
AAAGG 986395 0.73781204 8.407973 10-14
AGCCA 1073200 0.7327308 5.7784977 80-89
CTAAT 857460 0.7291802 9.184785 240-247
GTCGA 1382845 0.7276326 11.938535 70-79
ATGAT 948420 0.72136104 6.7080393 240-247
GGAAG 1300120 0.7100829 6.2718763 30-34
CATAC 879415 0.7085505 8.557611 70-79
GAAAA 690180 0.7070116 5.587482 8
GTAGC 1332515 0.7011495 5.383707 80-89
AATCC 867130 0.69865245 8.76805 240-247
AAGGT 1058280 0.68208635 9.415153 10-14
AAAGC 795665 0.66541743 9.202129 70-79
CATTG 1061170 0.65892607 5.2366824 50-59
TAGGT 1148245 0.63770115 5.339406 10-14
AGGAT 987005 0.6361479 5.6331725 10-14
TATGA 830410 0.63160354 5.5044417 240-247
ATAGG 969555 0.624901 5.135074 10-14
GAGGT 1327700 0.62484086 6.676004 10-14
AGAAG 834000 0.62382233 5.1316347 9
CAATC 774250 0.6238184 8.532061 240-247
TAAAA 500575 0.60512686 5.5962844 8
CGATG 1143880 0.6018927 13.239112 15-19
GGGTC 1566075 0.6017026 5.805599 70-79
AATGG 905000 0.5832938 6.4886465 10-14
AAATG 645645 0.56990415 5.0212135 9
CATCC 852950 0.56104845 7.0314364 240-247
GAAGG 985785 0.53840345 5.4136105 10-14
AAGGG 950215 0.5189763 6.165246 10-14
AAGCA 616780 0.5158154 6.0370874 70-79
GGTCG 1306720 0.5020556 5.8787622 70-79
TGATC 797065 0.49493194 5.2047925 240-247
GATCC 793205 0.466652 5.1412883 240-247
ATAAG 528630 0.46661624 5.124778 9
ACGAT 636710 0.4588276 5.886074 10-14
CGGTT 998940 0.4529204 8.0152 10-14
TAAGG 670010 0.4318372 5.617254 10-14
AAGCT 564115 0.40651402 7.932967 30-34
TAAAG 460370 0.40636384 5.560836 9
Produced by FastQC (version 0.10.1)