FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_1_SL248338.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_1_SL248338.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6705845
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31319 0.4670403207947693 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25315 0.37750648874228376 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24870 0.3708704868663084 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24662 0.3677687152029312 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24268 0.3618932438790339 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23297 0.34741333866201796 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 22761 0.3394203116833151 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21921 0.3268939261196762 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20888 0.3114894543491536 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20554 0.3065087248512305 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19270 0.2873612497753825 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19056 0.28417000392940783 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19034 0.28384193192655066 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18878 0.2815156031790177 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18876 0.28148577845148526 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18441 0.27499890021317225 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17999 0.2684076354284956 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17808 0.26555937394914436 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17573 0.2620549684640787 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17410 0.2596242531701821 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17353 0.25877424843550667 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17302 0.2580137178834286 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16931 0.2524812309261547 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16864 0.2514821025538168 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16753 0.2498268301757646 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15820 0.23591359478186566 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15699 0.23410919876615102 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 15466 0.23063461800861784 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15288 0.2279802172582277 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15216 0.22690652706705863 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15159 0.22605652233238316 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15141 0.2257880997845909 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14837 0.22125474119965494 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14704 0.21927139681874544 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13921 0.20759501598978208 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13649 0.20353885304536568 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13605 0.20288270903965122 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13552 0.2020923537600407 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13320 0.19863268536627374 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13276 0.19797654136055934 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13200 0.19684320171432534 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12860 0.19177299803380482 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12608 0.18801508236471318 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 12305 0.18349663614354342 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12127 0.18084223539315328 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12110 0.18058872520912728 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11768 0.1754886968010743 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11513 0.1716860440406839 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11508 0.17161148222185274 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11338 0.16907638038159248 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 11115 0.1657509232617217 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11010 0.1641851250662668 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10871 0.1621123065027599 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10826 0.16144125013327926 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10423 0.15543156753548584 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10362 0.1545219133457454 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10279 0.15328418715314776 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9793 0.14603677836275666 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9531 0.14212973905600262 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9517 0.14192096596327533 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9429 0.1406086779518465 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9226 0.1375814681073004 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8984 0.13397267607587113 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8909 0.13285424879340338 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8159 0.1216699759687258 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7945 0.11847873012275113 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7886 0.11759890066054315 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7763 0.11576467991729603 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7703 0.11486993809132183 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 13829770 7.782168 66.816925 35-39
TATTA 6904125 7.58184 131.36723 240-247
TGGAT 10481495 7.3376045 80.48621 35-39
TCACC 10813100 6.583742 76.738594 160-169
TCTCC 9445940 6.139889 85.74921 20-24
CTGTG 10058680 6.042538 87.87841 25-29
TCCCT 9178480 5.966039 74.87765 15-19
AGTGG 10853840 5.871521 40.663876 100-109
CTGAG 10200215 5.7397766 50.40078 15-19
TGTAT 6333825 5.738005 61.95959 240-247
TTACT 5872615 5.534073 115.395325 240-247
ATTAC 5795900 5.116129 109.20536 240-247
CCTGA 8465205 4.9549766 51.912785 15-19
TGGGT 8560715 4.9439034 46.264336 100-109
CTCTG 7694585 4.8081946 75.57548 35-39
TTCAC 6328565 4.7937264 77.68461 40-44
ACACG 8657520 4.7468433 50.9648 230-239
CTCCA 7729525 4.706255 42.03652 80-89
GACAC 8512010 4.6670613 47.46659 230-239
TGAGA 6934245 4.5471315 96.19078 15-19
TACTG 6081015 4.4281836 80.97571 240-247
AAGGG 8725600 4.4215016 40.920887 90-99
ATTCA 5008075 4.420704 115.488686 40-44
TCTGG 7159940 4.301182 137.76202 35-39
GAAGG 8403015 4.258039 33.009476 90-99
CCCTG 8401650 4.2200356 87.50583 10-14
AGCCT 7206710 4.218336 52.92169 30-34
ACCAT 5942000 4.2160707 70.323784 160-169
TGGAG 7783320 4.210485 39.770332 90-99
ACTAC 5893545 4.18169 37.364655 130-139
CAGCC 8878310 4.177237 57.065414 30-34
TCAGT 5733105 4.1748357 97.01225 7
CCAGG 9217540 4.169225 54.656963 80-89
TACTA 4703585 4.1519265 31.49732 130-139
GAGAC 7859440 4.14271 92.85505 8
CACCA 7140470 4.0724587 59.474186 160-169
GTATT 4467010 4.0468006 71.95313 240-247
TCCAG 6870580 4.0215874 41.37836 80-89
GGGTC 8538035 3.9634423 128.22276 8
ACTGT 5380065 3.9177527 69.55185 240-247
CACCT 6391210 3.8913987 45.11179 20-24
CAAGA 6006620 3.837905 46.659306 180-189
TGAAG 5815630 3.8135998 41.259754 150-159
CTGGG 8172400 3.7937105 135.31792 3
AGACT 5538805 3.7780948 70.763756 15-19
GACTC 6437880 3.7683132 52.16438 20-24
GATTC 5144725 3.7463787 59.707077 40-44
GCTGG 7873930 3.6551583 34.612896 90-99
GGACA 6849615 3.610432 29.105923 220-229
ACTGG 6375845 3.5877604 46.03664 60-69
CCTGT 5641275 3.5251217 60.707737 25-29
ACTCT 4555075 3.450353 68.83489 20-24
TATCT 3660315 3.4493067 51.02345 200-209
GGATT 4850230 3.3954196 57.1466 40-44
AGACA 5306620 3.390643 40.052303 170-179
GAGTG 6253305 3.382804 39.5615 100-109
AGCTG 5952240 3.349393 38.182476 60-69
TCCTG 5268550 3.2922132 63.236584 20-24
CTGCA 5517835 3.2297792 47.309315 25-29
CTCCT 4952010 3.218821 92.89331 20-24
GGTCC 6662340 3.2170653 143.12064 9
AGTAG 4890490 3.2069387 31.779068 50-59
GTCCC 6245135 3.136847 75.15922 10-14
GTCTC 5003800 3.1267762 35.300617 30-34
CATCT 4119555 3.1204576 32.644123 170-179
CCTCA 5124535 3.1201618 81.71037 5
AAGAA 4171450 3.1060164 40.69624 180-189
CCATC 5078610 3.092199 37.663776 45-49
GGAGT 5620270 3.040356 38.10678 90-99
GGCTG 6509910 3.0219665 35.91929 90-99
GAGCT 5280215 2.971237 31.49482 60-69
AGGGA 5823940 2.9511511 42.407543 80-89
TGTGC 4724260 2.8379989 58.55667 25-29
GGAAG 5600190 2.837771 31.907053 80-89
CAGGG 6441120 2.8008077 37.855186 80-89
ATCTG 3834630 2.7923703 38.23227 200-209
GTGAA 4247755 2.7854657 40.104904 150-159
GAACA 4344650 2.7759962 36.38356 210-219
GTGTA 3959540 2.7718887 37.744026 230-239
GACAA 4325325 2.7636485 25.292887 180-189
ATGAA 3469750 2.7580848 28.317823 210-219
ACCTT 3613045 2.7367892 73.39667 40-44
CTATG 3729380 2.715727 28.0836 50-59
ATCTC 3545540 2.6856558 48.853043 170-179
GGGAA 5275850 2.6734188 35.20621 80-89
AGAAC 4177330 2.6690876 32.09938 190-199
GAGTC 4731730 2.6625984 29.391108 150-159
CACGG 5747525 2.5996878 45.442314 230-239
CCCTC 4954200 2.5884724 28.41306 20-24
CGCCA 5492105 2.5840304 40.34829 70-79
TGCAA 3773085 2.573673 37.361313 200-209
ATCAG 3765750 2.56867 29.693956 45-49
CCAAG 4620995 2.5336516 41.17945 180-189
AGGGC 5808295 2.5256345 33.350365 150-159
CTACA 3551860 2.5201774 19.516329 190-199
CAGTG 4467805 2.5140846 74.91141 8
AGGCT 4452835 2.5056608 20.576468 25-29
ACGGC 5506075 2.4904768 50.246964 230-239
CTGTC 3965405 2.4779036 52.183556 30-34
CTACT 3262650 2.471374 23.141815 50-59
TCTGT 3165590 2.4609165 26.458426 210-219
CACTG 4185530 2.4499352 20.978373 30-34
GTGGA 4522960 2.4467523 27.769325 100-109
TATAT 2224245 2.442579 24.656267 240-247
TGAAC 3526760 2.4056516 38.980915 210-219
GGGCT 5161740 2.396132 43.771523 90-99
GCAGC 5289260 2.392408 34.988922 30-34
CCGCC 5903820 2.3836322 34.830647 70-79
GTAGT 3376060 2.3634217 28.407127 120-129
CTTCA 3118530 2.3622067 52.50887 45-49
CAAAT 2847825 2.3547304 37.103096 200-209
GTATC 3233090 2.3543298 23.733704 200-209
CAGTA 3426615 2.3373413 22.757236 45-49
CTCTC 3574240 2.3232665 98.30707 20-24
ACAAC 3487850 2.3181436 21.083658 180-189
GTGGT 4005890 2.313444 32.727917 120-129
TCCAA 3258875 2.3122935 45.060783 180-189
ACAGC 4205185 2.3056667 28.213375 210-219
GGCCG 6169750 2.302167 25.90027 230-239
TATGC 3124995 2.2756152 21.12491 50-59
TGGTG 3937280 2.2738209 36.62749 35-39
AAATG 2857935 2.2717564 35.230274 200-209
AATGA 2838435 2.256256 26.768236 210-219
CAGGC 4980895 2.2529304 33.557358 70-79
GCTCC 4462720 2.2415643 34.401775 80-89
TACAT 2514405 2.2195036 18.474949 190-199
GCCTC 4414675 2.2174318 71.386505 4
TATCA 2501335 2.2079666 19.078032 170-179
GTGGG 4849185 2.1640382 30.490614 100-109
GGGGG 6273020 2.1632614 96.87867 5
GCCTG 4478400 2.1624992 30.983484 210-219
CCTTC 3320730 2.1584842 57.2118 45-49
GCTAT 2961075 2.1562493 34.729225 50-59
TCTAT 2266515 2.135856 20.76176 110-119
GCTGT 3553400 2.1346297 20.09441 230-239
ATATC 2364875 2.087511 14.969692 170-179
AACAG 3258070 2.0817306 35.176018 210-219
GCAGA 3939050 2.0762732 25.395414 140-149
CCTCT 3193105 2.0755277 49.517513 35-39
CATAT 2342725 2.067959 15.71891 160-169
TGAGC 3672710 2.0666757 18.514452 200-209
AACTA 2496665 2.064373 37.790142 130-139
TGCAG 3663560 2.0615268 44.419277 30-34
AGCAC 3755315 2.0590067 22.777327 130-139
CTCAC 3344405 2.0362985 57.251064 20-24
TGTGA 2906625 2.0347922 20.235214 210-219
CAGAG 3837295 2.0226383 33.41521 170-179
TGCAC 3455505 2.0226264 28.350424 25-29
AGAGT 3056575 2.0043492 32.22792 150-159
GGCTC 4149175 2.0035253 21.305357 40-44
TGGTA 2847670 1.9935206 32.274754 120-129
TAGTA 2347980 1.9924921 19.8119 120-129
TCCGC 3950625 1.9843456 43.14343 70-79
ATGGA 2999190 1.966719 12.799761 100-109
CCGTG 4055170 1.9581327 20.756712 230-239
CTGTA 2686080 1.9559982 25.833178 190-199
TTCAG 2662700 1.9389732 48.68913 45-49
TCCAT 2537640 1.9221975 28.749943 45-49
GGTCT 3199275 1.9218966 35.0879 15-19
CTATT 2037825 1.9203492 21.058748 110-119
TCTCT 2370605 1.9169902 34.72584 35-39
TGGGG 4290980 1.9149288 126.14619 4
CAGAC 3488795 1.9128766 27.429226 140-149
CAGTT 2618145 1.9065282 26.751524 190-199
ACCAG 3471650 1.9034758 15.16375 190-199
GCACA 3468730 1.9018747 19.260408 130-139
GCCAG 4198845 1.8991977 36.048496 70-79
GTCAC 3233410 1.8926264 39.007343 160-169
GGTGG 4237340 1.8909911 19.680803 40-44
ACCCT 3092745 1.8830712 45.151264 10-14
GGATC 3334870 1.8765696 19.273497 70-79
TGGGA 3468750 1.8764641 16.68129 100-109
TAGTG 2678880 1.8753586 28.576233 120-129
ATACT 2119805 1.8711841 28.797068 130-139
ACTAT 2117840 1.8694497 17.467031 130-139
AGAGA 3042880 1.869091 29.165157 170-179
GCTGA 3306545 1.8606308 27.912432 200-209
TGTCT 2390040 1.8580073 53.41533 30-34
CCAGA 3374990 1.850478 35.056282 170-179
CCTGC 3658280 1.8375046 49.974865 20-24
AGGAC 3477815 1.8331561 21.223818 220-229
GATGG 3377730 1.8272256 20.760168 100-109
TACAA 2207830 1.8255491 18.893845 130-139
GCCGA 4032410 1.8239167 20.38154 220-229
AGTCA 2673590 1.8236923 30.735802 160-169
GAGGA 3558460 1.8031698 27.807772 220-229
AGTTC 2459075 1.7906936 23.72869 190-199
CACAG 3260255 1.7875696 15.83385 140-149
CATGA 2618655 1.7862207 25.053247 60-69
TTCCA 2346580 1.7774744 23.360685 180-189
GCAAA 2761040 1.7641551 33.953053 200-209
TCAGC 3008225 1.7608179 11.955667 45-49
TGACC 2996360 1.753873 14.825336 210-219
ACCTG 2972580 1.7399536 32.04984 25-29
AGCTA 2546340 1.7368934 26.958693 50-59
CAAGG 3267770 1.7224416 17.248028 80-89
GGAGC 3945005 1.7154162 12.984894 200-209
ATTGG 2446715 1.7128305 21.945147 100-109
GTGCA 3031180 1.7056794 52.046577 25-29
CTGAA 2497025 1.7032549 25.198828 200-209
AACAC 2551330 1.6957006 23.037813 190-199
AGGGG 4032370 1.6856362 34.72652 90-99
TTCTG 2160985 1.6799409 42.279655 30-34
CTCAG 2860260 1.6742089 78.22185 6
GGATG 3072870 1.6623079 22.521395 100-109
GGGGC 4629315 1.6606096 29.950363 90-99
ATGCA 2412615 1.6456779 15.836453 60-69
AGTTA 1929405 1.6372898 17.539427 50-59
TCTGC 2606865 1.6289786 34.50266 200-209
CCTGG 3361920 1.6233808 35.0509 2
GCCGT 3328850 1.6074122 30.147139 230-239
CGATT 2197155 1.5999639 35.552395 160-169
CGTCC 3177775 1.5961536 19.966114 180-189
GGGCC 4245845 1.5842851 28.770811 2
CTCCC 3029675 1.5829457 14.337061 190-199
GTTCT 2030635 1.5786073 24.871971 190-199
TTAGT 1733280 1.5702312 27.62838 110-119
GTTAC 2153705 1.5683237 17.060228 50-59
TTCTC 1918910 1.5517269 23.42048 190-199
ACAAT 1874380 1.5498353 23.85971 180-189
CAGCT 2639890 1.5452188 12.907399 50-59
TCTCA 2039530 1.5448918 27.30073 100-109
GCACT 2638390 1.5443407 27.424053 25-29
AGTGA 2328135 1.5266746 87.07694 9
CGGCC 3888370 1.5092295 31.923426 230-239
GAGCA 2861485 1.5082887 7.7077756 200-209
CCATG 2571590 1.5052403 15.915073 160-169
GGCTT 2495030 1.4988364 21.739302 25-29
GTCCG 3099520 1.4966749 34.585205 70-79
TCTGA 2045480 1.4895145 21.001762 220-229
CATCA 2087740 1.4813296 34.041637 45-49
CTCAA 2069045 1.4680647 20.540098 150-159
CTTTA 1556035 1.4663333 42.282032 45-49
TAGCA 2121350 1.447002 12.335396 130-139
CCATA 2024015 1.4361143 16.141586 160-169
GTGAC 2548160 1.4338787 16.020224 210-219
AAGCT 2099190 1.4318863 18.345482 200-209
ACTCC 2343750 1.4270327 17.399426 140-149
TACGC 2437135 1.4265393 25.661388 140-149
CAACT 2008215 1.4249034 18.579847 130-139
CTGGT 2365455 1.4209967 33.310925 35-39
AACCC 2474100 1.4110653 21.867418 140-149
CAACC 2469215 1.4082793 16.692436 140-149
GGGGT 3153975 1.4075195 120.697334 7
GTCCA 2394025 1.4013054 29.52373 180-189
ATCTA 1567280 1.383462 21.65846 110-119
CACGT 2347465 1.3740524 17.93027 180-189
CGTGT 2282730 1.3713017 24.668116 230-239
TCAAG 2006250 1.3684906 25.38166 150-159
GAGAG 2699220 1.367769 26.862259 220-229
GGCAG 3143660 1.3669653 13.504517 150-159
GAACT 1998500 1.3632042 15.549574 190-199
CTACG 2327795 1.3625387 16.456688 130-139
AGCAG 2578445 1.3590983 13.943095 50-59
CCAGT 2295200 1.3434598 20.442059 190-199
TGTCC 2149675 1.3432896 56.957924 15-19
TGTGT 1782145 1.3318853 15.806115 240-247
CAATT 1508345 1.3314391 24.882727 180-189
AAGAG 2164335 1.3294443 20.627306 150-159
TTACA 1498940 1.3231372 10.038651 120-129
GATTG 1879435 1.3157043 25.47704 100-109
AACCA 1978350 1.3148786 19.04841 190-199
ACATA 1575500 1.3027056 22.485092 130-139
AATTC 1474775 1.3018063 24.860704 180-189
ATACA 1573195 1.3007997 22.458439 35-39
CGACA 2367795 1.2982415 16.270073 70-79
CCTTT 1602785 1.2960924 48.939484 45-49
AGAGC 2454335 1.2936798 25.884382 220-229
GTGGC 2779310 1.2901838 15.2587185 40-44
ACAGT 1889465 1.2888299 12.998078 120-129
TGGAA 1962875 1.2871554 19.577034 120-129
TCCGT 2055505 1.2844447 13.856986 150-159
ACGTC 2190445 1.2821431 19.690754 180-189
TCACT 1692485 1.2820141 14.708635 190-199
GTGCG 2753895 1.278386 17.271576 240-247
ATCCG 2184005 1.2783736 25.54175 70-79
TTATT 1088655 1.2762887 20.478855 240-247
GGCCT 2638990 1.2742975 68.452705 3
ACATG 1865115 1.2722206 14.498723 190-199
AGACC 2314555 1.2690505 104.335815 9
TGCGA 2248800 1.2654252 14.555394 240-247
ACAGA 1973550 1.2609916 19.986538 140-149
AGTCG 2228340 1.2539121 17.004261 150-159
ATATT 1137780 1.2494656 26.317408 240-247
CCTAC 2037295 1.2404422 16.90094 190-199
ATTAG 1450370 1.2307814 26.966127 110-119
GATCC 2097160 1.2275401 25.38069 70-79
CGGCT 2538815 1.2259256 21.93187 230-239
GTCGA 2161950 1.2165537 13.25832 150-159
ACACA 1829015 1.2156254 7.7824597 130-139
ATTAT 1106665 1.2152963 8.731606 50-59
CTTCG 1925530 1.2032259 110.84052 3
AGCTC 2032855 1.1899002 13.754745 210-219
CTTCT 1463835 1.1837305 22.676163 30-34
ACGCA 2158940 1.1837282 22.1891 140-149
TGGAC 2103425 1.1836212 15.831483 80-89
TACCA 1667205 1.1829442 10.543512 45-49
TCGGA 2097855 1.1804868 99.19044 5
TTGGG 2043990 1.1804259 16.460913 100-109
TCGAG 2075255 1.1677694 12.8013525 150-159
GAACC 2120775 1.1628026 11.257561 180-189
ATCAT 1309220 1.1556685 15.700839 110-119
ATTCC 1523720 1.154179 22.836378 180-189
TAGAC 1686090 1.1501052 18.74326 170-179
TGAGT 1641600 1.1492074 20.818602 90-99
TTCGG 1911980 1.1485814 106.69173 4
GAAGC 2173415 1.1456069 14.304545 200-209
CGCTG 2369995 1.144407 12.787065 190-199
GAAGT 1744095 1.1436905 13.6394 140-149
GGACT 2002675 1.126928 13.043493 90-99
GACCC 2393580 1.1261772 44.358963 10-14
GGGTG 2508500 1.1194643 14.289089 60-69
CGCAG 2472345 1.118277 17.46965 140-149
GTATA 1308055 1.1100134 10.394539 240-247
CCGAT 1889190 1.1058081 24.148773 160-169
GCCCC 2721485 1.0987834 12.868613 80-89
GCACC 2327175 1.0949337 12.256044 130-139
GCAAG 2074790 1.0936217 16.503958 25-29
ATCAA 1317940 1.0897416 13.138263 110-119
GGAGA 2146850 1.0878681 89.1324 7
GATCT 1490705 1.0855285 9.256962 220-229
GCTTC 1735760 1.0846423 29.107834 2
CACAT 1526395 1.0830342 14.7248335 130-139
GCTCT 1731855 1.0822022 18.961706 210-219
CTATA 1223280 1.0798079 14.281998 50-59
TAGTT 1191275 1.0792124 15.94664 50-59
ACAAG 1685260 1.07679 17.593737 80-89
AAGGC 2042840 1.0767808 28.878145 25-29
ATGAG 1639235 1.0749284 21.210947 60-69
GTAGC 1893285 1.065373 12.171059 50-59
GAGCC 2350650 1.0632327 18.96683 220-229
CCCCT 2025290 1.0581743 9.691368 140-149
GGGAG 2528130 1.0568244 13.035017 5
AGGCC 2335690 1.056466 13.770512 2
CTTGA 1449770 1.0557196 21.876816 90-99
ACACC 1849720 1.0549597 13.247767 40-44
GTAGA 1606785 1.0536494 18.818354 170-179
AGGTC 1863745 1.0487505 18.0384 10-14
TTGAG 1496965 1.0479552 20.556301 90-99
CAGCA 1887285 1.0347822 12.626658 45-49
ATGGT 1475915 1.0332191 10.622564 35-39
TGGCT 1719410 1.032899 33.444653 40-44
ATGGG 1905080 1.030577 19.368385 100-109
CTCCG 2050140 1.0297576 10.046695 140-149
CGAGT 1829740 1.0296155 16.421873 160-169
CGTGA 1826720 1.0279163 20.93255 150-159
ACCGC 2167140 1.0196375 12.189924 220-229
CGAGG 2335685 1.0156314 15.37253 220-229
GCCAA 1852155 1.0155207 15.738527 180-189
TTATA 924270 1.0149972 13.461963 110-119
AGCCG 2241845 1.0140185 20.006357 220-229
ACTCA 1404485 0.9965346 13.566271 190-199
AACTC 1400445 0.993668 17.280622 190-199
TTTAG 1096060 0.9929542 31.309029 45-49
AGATC 1450890 0.98967206 12.629996 210-219
ACCAA 1482510 0.98532647 18.104982 130-139
GACAG 1865410 0.9832576 15.661741 70-79
TTTCA 1041030 0.9810171 7.983787 100-109
TGAGG 1808330 0.9782388 11.660334 220-229
AAACT 1182900 0.97808343 16.924473 130-139
TACAG 1431835 0.9766744 8.858575 120-129
CATAC 1376300 0.9765363 15.051154 130-139
ATGAC 1431260 0.9762822 11.306042 160-169
CCGAG 2156180 0.97527105 18.990122 220-229
TCATA 1102775 0.9734363 9.393512 110-119
CCGTC 1927250 0.96803176 10.8033285 140-149
AATCA 1168060 0.965813 14.969527 110-119
CCAAC 1684825 0.9609143 14.791375 130-139
AAGGT 1448775 0.9500343 20.174866 15-19
TAACA 1146070 0.94763047 9.263047 120-129
AGCCC 2010150 0.9457737 12.436457 80-89
GGTAT 1349035 0.94439644 9.429629 110-119
AACTG 1383885 0.94396704 12.987181 60-69
CGGAC 2072355 0.9373558 11.032318 220-229
TGGTT 1247400 0.9322439 10.176058 50-59
TACAC 1308655 0.92853963 22.969618 35-39
TATGG 1325065 0.92761594 13.721022 35-39
ACAGG 1759035 0.9271873 15.2600975 70-79
CCCAG 1966800 0.92537767 15.6061325 80-89
GGGAC 2121940 0.9226882 10.030104 90-99
GTTAT 1013050 0.9177529 10.738969 110-119
GCAGT 1630605 0.91756 7.119637 50-59
TTTAT 776705 0.91057307 11.121134 240-247
ATAGT 1070255 0.90821654 17.133226 120-129
CAGAA 1416750 0.90522665 18.022968 140-149
CGGAG 2074680 0.902138 76.656975 6
ATGAT 1061330 0.9006428 8.657684 120-129
CCCCA 1837405 0.89925355 17.568903 80-89
GATAC 1317455 0.8986541 24.213614 35-39
AAGTT 1056055 0.89616644 17.695122 140-149
GGTAG 1647165 0.8910547 12.828531 120-129
GCCAT 1513920 0.8861497 7.852065 60-69
GACTG 1569520 0.8831867 12.681436 90-99
GAGAT 1340275 0.87888545 12.250197 210-219
TATTT 739725 0.8672194 10.742703 240-247
CGTAT 1178425 0.8581269 7.6188273 240-247
AGAAG 1385335 0.85094297 16.026585 140-149
GTTTC 1089210 0.8467474 5.744183 100-109
TCCTT 1045990 0.84584 11.23829 45-49
TATAC 944760 0.83395404 10.223776 130-139
TCTTC 1030445 0.83326954 48.53586 2
ACGCT 1419230 0.83072436 15.750978 190-199
GTCTA 1137425 0.8282707 11.071663 30-34
GATGA 1245555 0.81677276 6.687192 60-69
ATATA 790620 0.8132818 12.217157 130-139
GGTTC 1351620 0.81195706 11.850578 160-169
CATGG 1442530 0.811728 8.335607 200-209
ATCCA 1143030 0.81102246 9.429835 180-189
TTACC 1068310 0.8092175 9.098339 45-49
GCCTA 1376310 0.8056018 16.203627 190-199
AGTCT 1091985 0.79518133 13.185178 8
TGGCA 1409115 0.792925 5.565102 100-109
CATTA 895365 0.79035234 13.279506 110-119
GATCA 1157350 0.78944427 8.790723 110-119
GCTTG 1309610 0.78672045 16.779394 90-99
CCTCC 1502605 0.7850816 10.508746 140-149
GTCTG 1304055 0.7833833 14.40935 1
TTATG 864465 0.78314525 11.966777 50-59
ATATG 921670 0.78212756 5.7495666 50-59
ACAAA 1009000 0.7814953 13.084382 130-139
GGTGC 1677925 0.7789098 11.40035 60-69
GGTTA 1111325 0.77798676 9.056193 50-59
GTTCA 1064690 0.77530515 14.43153 160-169
ACATC 1091250 0.77428263 5.931335 110-119
TCATC 1010150 0.7651628 9.486072 110-119
CCAGC 1623810 0.7640012 6.8430123 80-89
GACCG 1685795 0.76250917 9.599359 210-219
CCCCC 1804660 0.7579141 14.240273 80-89
CTATC 998770 0.75654274 7.4602914 110-119
GCGGA 1735845 0.7548016 9.626061 220-229
TATGA 883205 0.7494862 5.430961 50-59
AACGC 1358410 0.74480444 15.338428 180-189
TGATG 1053570 0.7375551 11.647321 120-129
ACGCC 1555765 0.73198605 13.648689 180-189
CAACG 1326710 0.7274236 15.609267 180-189
GAAAT 912840 0.7256114 8.042836 110-119
TCAAC 1022120 0.7252323 7.511014 110-119
TATAG 853215 0.72403675 6.961192 120-129
GGGAT 1338315 0.72397834 13.385849 100-109
CTGAC 1228590 0.7191361 9.385898 220-229
CGCCG 1846690 0.71677303 11.638924 220-229
ATAAA 741505 0.71448755 7.790648 130-139
ATCTT 753705 0.71025574 13.521913 1
TGACT 963420 0.70156056 5.2390904 2
TGCCA 1187390 0.6950203 7.5398407 60-69
GGGCA 1596235 0.6940946 13.514879 150-159
GACCA 1265875 0.6940683 6.169821 170-179
ATGCC 1184600 0.6933873 8.448882 50-59
GCCGC 1780880 0.69122964 12.836953 220-229
TATTG 759260 0.6878368 6.3527236 240-247
TTTAC 728525 0.6865273 12.413477 45-49
TCCGG 1420030 0.68569434 11.814367 70-79
CACAA 1029270 0.68408775 8.377297 130-139
CATCC 1123425 0.6840167 6.2723265 110-119
CCCGT 1352915 0.679551 10.649248 140-149
GGATA 1035990 0.67935055 15.094496 35-39
AATAC 820580 0.6784984 11.541535 130-139
TTGGA 967005 0.676955 5.952253 60-69
TTTCT 665715 0.6697217 9.49578 4
GATTA 781095 0.66283584 5.1909595 50-59
CAAAC 992540 0.65967584 13.605331 130-139
AAATA 683030 0.65814316 12.896593 130-139
CACGC 1396965 0.6572708 12.701966 190-199
TCAAT 730795 0.64508384 9.067462 110-119
TAGCT 884885 0.6443715 13.438293 50-59
AAGCA 1005115 0.64221406 6.2084737 120-129
GCGAC 1409745 0.6376478 7.056374 70-79
TACTT 667365 0.628893 11.634972 1
ACTTC 823045 0.62343556 31.156084 4
TTCAT 657375 0.6194789 5.141404 100-109
CCGGC 1589690 0.6170213 9.531142 70-79
CAGTC 1053700 0.616767 5.2059264 210-219
GACGA 1169150 0.6162589 9.679583 220-229
ACCCG 1305120 0.6140578 9.759857 140-149
ACCTA 849970 0.60308546 8.178565 130-139
AGGCA 1141800 0.60184276 6.092138 80-89
AGATG 908515 0.5957588 5.9192224 120-129
CCCTA 977230 0.59500337 11.796799 110-119
TTAGC 814050 0.5927896 10.371481 45-49
AATAA 610045 0.58781743 7.268606 130-139
TCCAC 957025 0.58270115 9.767611 180-189
CACCG 1229475 0.5784669 9.126061 45-49
CGGTT 953200 0.5726146 11.77706 160-169
CCGTA 970625 0.5681403 6.587135 230-239
AATGG 865890 0.56780744 6.5251384 120-129
ACGAC 1034850 0.56739944 9.040057 220-229
CACGA 1032965 0.56636584 8.428783 180-189
GGCCC 1450605 0.563037 9.856668 70-79
CTGCG 1144680 0.5527352 12.764752 25-29
GTTCC 883825 0.55228484 6.7282753 140-149
CGGCA 1220555 0.5520745 10.985582 70-79
TAATA 536235 0.5516053 7.9189844 130-139
GGGGA 1308030 0.5467907 7.6833944 4
CTTTG 702160 0.5458563 6.6807594 45-49
ATTTC 569705 0.53686285 6.343384 240-247
AAATC 648255 0.5360111 8.561631 110-119
ATTAA 514325 0.5290673 6.273567 110-119
TTCAA 595875 0.525988 5.4924016 200-209
ACATT 593930 0.5242711 6.9738145 110-119
CTGTT 672260 0.52261215 5.7850566 240-247
TCAGG 924025 0.51995933 5.258604 150-159
GGTGA 958915 0.51873726 5.8715577 120-129
TGATT 571425 0.51767135 5.6136084 50-59
TATGT 568800 0.5152933 6.9302087 140-149
GGAGG 1231460 0.5147825 5.4742427 6
CCACG 1085435 0.51069623 7.367172 180-189
ACCCC 1039335 0.5086661 9.218023 140-149
GCTAC 867165 0.50758165 7.7242417 50-59
GTCCT 811570 0.50713414 7.032297 45-49
AGTAT 595380 0.5052384 6.027393 110-119
CGCGG 1348100 0.503027 7.0083575 220-229
GGGTA 921650 0.4985782 5.297969 100-109
AAGAT 623830 0.49587896 5.556061 120-129
GTAAC 723815 0.49372417 7.306555 120-129
CATAA 588085 0.4862594 6.2241993 110-119
GAAGA 779290 0.47867942 5.333647 15-19
CAATC 670045 0.47542197 6.7790065 110-119
GGGTT 818665 0.4727877 6.1821766 100-109
GGTTT 631205 0.4717308 6.0248995 100-109
TGACG 838080 0.47159714 8.5796585 220-229
CCGGT 973190 0.4699273 9.143347 160-169
AGTTT 518235 0.4694849 5.002795 140-149
CCGCG 1193620 0.46329087 7.282931 220-229
AACAT 555255 0.45911384 6.0883913 110-119
TGGCC 940070 0.45393455 14.396097 2
CATAG 660540 0.45056343 5.1860905 120-129
CCATT 594570 0.45037156 5.2272177 110-119
TAAAT 418085 0.4300687 7.7651424 130-139
GCATG 760875 0.42815298 8.734403 60-69
TGCGC 886675 0.4281515 9.513 25-29
ATGCT 582320 0.42404428 6.504518 50-59
GCGCT 864875 0.4176249 8.683662 25-29
TGCTA 570310 0.4152986 5.7650757 50-59
CTAAC 581285 0.41244343 5.966822 110-119
GCCTT 656545 0.4102621 10.031575 1
CCTAA 572400 0.4061392 8.158991 110-119
ATTCT 429530 0.40476862 7.461902 2
ATGGC 691560 0.38914868 6.950428 1
CACTT 506135 0.38338432 7.217081 3
ACGAG 724430 0.38184708 7.325673 180-189
GGTAA 580540 0.38068917 7.6243234 120-129
CATGC 619470 0.3625972 6.48729 60-69
TACCT 476955 0.36128122 5.1634364 2
AGCTT 495715 0.3609787 7.6119475 1
TTTGA 397675 0.3602659 5.744975 45-49
TTCTT 349330 0.3514325 20.187767 1
GAGGC 802365 0.34889427 5.293834 35-39
ATGTG 483295 0.33833224 5.253702 140-149
TGGGC 715860 0.33230945 9.209151 1
TGCTG 542265 0.32575417 14.223721 1
CGAGC 704430 0.31862375 6.1386094 180-189
CTCTT 376305 0.30429912 9.174576 1
CGCTT 473420 0.29583088 6.113329 1
GCGAG 666895 0.28998753 5.358594 240-247
TCACA 389740 0.2765351 5.765034 5
GACTT 378575 0.27567756 7.40583 1
AACTT 306820 0.2708347 10.834027 3
ATTGT 297655 0.26965475 5.1729302 240-247
TTCCT 332225 0.2686538 6.4522443 4
CCCTT 411165 0.26725847 6.0477986 3
GAGGT 491855 0.26607513 6.9211125 7
CTCGG 533440 0.25758386 5.316058 6
GTCTT 329460 0.25612083 12.659682 1
TAACT 288845 0.25496787 7.2720504 2
AATCT 274260 0.24209347 7.696162 2
TTGGC 386790 0.23235586 7.1207705 1
TGTAA 266665 0.2262915 6.6130314 25-29
CGGGG 621825 0.2230586 7.742513 3
CCTCG 418745 0.21032996 5.727698 5
TGCTT 257920 0.20050594 11.752605 1
TCGGT 307335 0.18462496 6.3182354 7
TGCCT 283760 0.17731604 5.2653365 4
TAGGC 258635 0.14553685 6.799498 1
TTGCT 152250 0.11835852 5.6315484 4
Produced by FastQC (version 0.10.1)