FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_4_SL248350.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_4_SL248350.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11797639
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 58383 0.49487020241931456 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 48019 0.407022116882878 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 43642 0.3699214732710503 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 41359 0.3505701437380818 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 40968 0.34725592129069216 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 40449 0.34285673599607513 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39162 0.33194777361809424 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 37735 0.3198521331259585 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37269 0.31590219026027155 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34802 0.2949912266344139 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 34229 0.2901343226386229 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34145 0.28942231577012995 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31345 0.2656887534870324 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31118 0.2637646396876528 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30685 0.2600944138060166 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30312 0.2569327642590183 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29641 0.2512451855833188 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29134 0.2469477155556294 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29092 0.24659171212138292 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28616 0.24255700653325638 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28111 0.23827648905005486 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27961 0.23700504821346033 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27683 0.23464864452963854 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27236 0.23085975083658689 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26554 0.22507893316620387 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26067 0.22095098858339368 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25824 0.21889125442811058 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25438 0.2156194133419407 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25315 0.21457683185593324 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24933 0.21133889585873922 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24223 0.20532074256552518 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 23932 0.20285414734253185 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23869 0.20232014219116215 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23808 0.20180308958428037 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22997 0.19492883279442608 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 22711 0.19250461893265255 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 22692 0.19234356976001724 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22411 0.18996173725946353 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22360 0.1895294473750214 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21969 0.1862152249276317 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20749 0.17587417278999637 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20446 0.17330586230007547 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20250 0.17164451294025862 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20172 0.1709833637052295 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20105 0.17041545346488396 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 19909 0.1687541041050671 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19386 0.16432101372147428 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 19344 0.16396501028722782 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19238 0.1630665254293677 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19166 0.16245623382780233 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18689 0.1584130519674318 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18273 0.154886922713943 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17858 0.1513692697326982 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17786 0.15075897813113284 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17652 0.14962315765044176 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17189 0.1456986436014867 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16777 0.1422064194369738 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16643 0.1410705989562827 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16549 0.14027382936535016 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16450 0.13943467841319776 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 16409 0.13908715125119528 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16341 0.13851076473860574 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15712 0.13317918949715277 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15710 0.13316223695266485 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13693 0.11606559583659068 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13604 0.11531120760687795 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13498 0.11441272274901784 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13029 0.11043735106659901 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12761 0.1081657101052168 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12584 0.10666540991803529 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11993 0.10165593302185294 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 24229105 7.7630625 66.12783 35-39
TATTA 11953330 7.4580936 128.86832 240-247
TGGAT 18345965 7.320416 79.74976 35-39
TCACC 18655175 6.4462037 74.55864 160-169
TCTCC 16385080 6.0564404 85.148346 20-24
CTGTG 17345655 5.944982 85.64967 25-29
TCCCT 15926985 5.887114 73.30921 15-19
AGTGG 18968440 5.8522506 40.268406 100-109
CTGAG 17539700 5.6197615 49.50524 15-19
TGTAT 10887970 5.6188316 60.434605 240-247
TTACT 10211415 5.4725475 114.8631 240-247
ATTAC 10214955 5.1177135 107.534874 240-247
TGGGT 14915480 4.922578 45.590134 100-109
CCTGA 14500705 4.824911 50.762802 15-19
CTCTG 13319860 4.740935 73.66963 35-39
ACACG 15019640 4.6719227 49.151085 230-239
GACAC 14952965 4.6511836 46.12065 230-239
TTCAC 10780840 4.6393456 75.07557 40-44
CTCCA 13182775 4.555243 40.54331 80-89
TGAGA 11821015 4.4094644 93.35537 15-19
TACTG 10626750 4.403522 80.770485 240-247
AAGGG 15005300 4.327846 39.90531 90-99
ATTCA 8577565 4.297377 112.34256 40-44
TCTGG 12520540 4.2912416 136.092 35-39
TGGAG 13786310 4.2534304 39.581768 90-99
CCCTG 14703885 4.2023964 85.233765 10-14
GAAGG 14464930 4.171992 31.5855 90-99
TCAGT 9939885 4.118898 96.59596 7
AGCCT 12351235 4.109705 50.690628 30-34
CAGCC 15371795 4.1070065 54.814182 30-34
CCAGG 15912500 4.0938764 53.24697 80-89
ACCAT 10162415 4.0882454 68.42154 160-169
ACTAC 10103650 4.0646048 36.78965 130-139
GTATT 7781900 4.0159173 70.121506 240-247
GAGAC 13407600 4.015898 91.81984 8
TACTA 7978755 3.9973726 29.007235 130-139
CACCA 12373960 3.9971397 57.856083 160-169
TCCAG 11712625 3.8972156 40.010998 80-89
GGGTC 14556595 3.8575876 121.709984 8
ACTGT 9283625 3.8469563 69.95506 240-247
CACCT 11058315 3.8211462 44.843575 20-24
TGAAG 10083695 3.761411 39.254913 150-159
CTGGG 14060025 3.725993 129.56651 3
CAAGA 10201550 3.6943462 45.158154 180-189
GACTC 11089015 3.6897182 51.026905 20-24
GATTC 8864610 3.6733246 59.20582 40-44
AGACT 9440330 3.6569812 68.566795 15-19
GGACA 12132660 3.6340232 28.553722 220-229
ACTGG 11246750 3.6034853 45.40396 60-69
GCTGG 13420010 3.5563855 32.882458 90-99
CCTGT 9835330 3.500687 60.496254 25-29
GAGTG 11200395 3.455609 39.74135 90-99
ACTCT 7847710 3.3771245 67.36797 20-24
AGCTG 10427845 3.341107 37.941063 60-69
GGATT 8349035 3.331436 56.53271 40-44
TATCT 6207390 3.3266923 48.4058 200-209
AGACA 9147905 3.3127835 38.965427 170-179
TCCTG 9217255 3.2806957 63.101353 20-24
CTGCA 9653255 3.2119884 47.279884 25-29
CTCCT 8688340 3.2114832 92.25789 20-24
GGTCC 11496760 3.1639957 137.93314 9
GTCTC 8816420 3.1380265 34.57854 30-34
CCATC 9063365 3.1318011 37.083706 45-49
CATCT 7270220 3.128612 32.106525 170-179
CCTCA 8967915 3.0988188 81.37131 5
GTCCC 10696780 3.0571585 73.055405 10-14
GGAGT 9829575 3.032676 38.00122 90-99
AAGAA 7124000 3.0035207 39.003204 180-189
AGGGA 10279870 2.9649322 41.110012 80-89
GAGCT 9187315 2.9436376 30.872355 60-69
AGTAG 7867575 2.9347558 29.398745 50-59
GGCTG 10913085 2.8920348 33.941753 90-99
TGTGC 8240290 2.8242452 56.91777 25-29
GGAAG 9766430 2.8168452 30.937822 80-89
GTGTA 7051495 2.813691 37.604065 230-239
CAGGG 11209455 2.7770028 36.722816 80-89
ATCTG 6640505 2.7516983 36.299694 200-209
GTGAA 7368775 2.748694 39.19662 10-14
CTATG 6565050 2.720431 28.361006 50-59
ACCTT 6321025 2.7201424 72.45648 40-44
GACAA 7505680 2.7180755 24.4215 180-189
ATCTC 6297985 2.7102277 47.21412 170-179
GGGAA 9258750 2.6704195 34.51041 80-89
GAACA 7353745 2.663054 34.665497 210-219
GAGTC 8159760 2.6144066 29.578587 150-159
CCCTC 8774210 2.6042187 27.85352 20-24
ATCAG 6695250 2.5935962 28.851448 45-49
AGAAC 7137180 2.584628 30.885015 190-199
ATGAA 5717330 2.578482 27.498226 210-219
CACGG 9974275 2.5661242 44.10652 230-239
CAGTG 7988260 2.5594573 74.684235 8
TATAT 4050190 2.5270529 24.53721 240-247
TGCAA 6513970 2.523372 35.344303 200-209
CTACA 6260325 2.5184705 19.90888 190-199
AGGGC 10160135 2.517047 32.646603 150-159
CCAAG 8015065 2.49312 39.490932 180-189
CGCCA 9291035 2.4823608 39.127518 70-79
GTGGA 7982935 2.4629402 27.68 100-109
AGGCT 7660875 2.4545627 20.613035 25-29
ACGGC 9526150 2.4508328 48.974155 230-239
CTGTC 6885135 2.4506247 51.251328 30-34
CTACT 5632490 2.4238434 24.188688 50-59
TCTGT 5456315 2.4185956 26.023401 210-219
CACTG 7266800 2.4179282 20.5738 30-34
GCAGC 9264650 2.383556 34.217194 30-34
TATGC 5705805 2.3643763 22.128355 50-59
CCGCC 10152920 2.330003 33.280018 70-79
TCCAA 5788960 2.3288448 44.20419 180-189
GTGGT 7040195 2.3234859 31.774008 120-129
TGGTG 7001585 2.3107436 36.243828 35-39
CTTCA 5365830 2.309091 50.918915 45-49
TGAAC 5919870 2.2932305 37.49127 210-219
ACAGC 7359230 2.2891197 26.554651 210-219
GGGCT 8612135 2.282269 42.22313 90-99
CTCTC 6170435 2.2807865 95.44295 20-24
CAAAT 4843925 2.2686775 35.113544 200-209
GTATC 5461610 2.2631867 22.675995 200-209
CAGTA 5840050 2.2623098 22.566248 45-49
GGCCG 10556750 2.2463984 25.53409 230-239
CAGGC 8694605 2.2368977 33.114788 70-79
ACAAC 5917995 2.2256174 19.600115 180-189
TATCA 4419930 2.2143939 18.139595 170-179
GCCTC 7723765 2.207466 71.49335 4
TACAT 4369205 2.1889806 18.64943 190-199
GCTCC 7648100 2.1858406 33.251335 80-89
AATGA 4841490 2.1834834 25.905788 210-219
AAATG 4834820 2.1804752 33.482178 200-209
CCTTC 5840110 2.158688 55.539764 45-49
GCCTG 7803880 2.1476872 30.288961 210-219
GCTAT 5169635 2.1421978 34.764412 50-59
GTGGG 8371465 2.1362543 29.831326 100-109
GTAGT 5288780 2.1103315 25.193764 120-129
TCTAT 3931220 2.106837 21.37818 110-119
GGGGG 10600185 2.091515 92.30133 5
AACTA 4438500 2.0787945 38.66685 130-139
TGCAC 6230145 2.0729954 28.091097 25-29
GCAGA 6916715 2.0717223 24.085974 140-149
GCTGT 6028365 2.066139 19.271206 230-239
TGCAG 6422850 2.0578964 43.23893 30-34
CCTCT 5515540 2.0387168 48.10021 35-39
ATATC 4053830 2.030977 14.850664 160-169
CTCAC 5862430 2.0257337 56.74532 20-24
AGCAC 6506635 2.0239165 21.134968 130-139
AACAG 5576025 2.019278 33.40439 210-219
TGTGA 5055025 2.0170586 20.280697 210-219
AGAGT 5401430 2.0148365 31.9607 150-159
TGAGC 6273040 2.009897 18.750511 200-209
ATGGA 5343710 1.9933059 12.47865 100-109
TGGTA 4987220 1.9900029 31.506557 120-129
CATAT 3941055 1.9744765 15.612428 160-169
GGCTC 7141780 1.9654721 21.23391 40-44
CAGAG 6550100 1.9619119 31.903284 170-179
TCTCT 4222895 1.9439209 33.77888 35-39
TTCAG 4672120 1.9360371 47.2669 45-49
CCGTG 7031585 1.9351457 20.746487 230-239
GGTGG 7577775 1.9337182 19.216578 40-44
TCCGC 6756470 1.9310111 41.244144 70-79
GGATC 6011015 1.9259436 18.714964 70-79
TCCAT 4469975 1.9235755 28.377262 45-49
GGTCT 5598075 1.9186627 35.931477 15-19
ACCAG 6144855 1.9113832 14.485666 190-199
CAGAC 6132740 1.9076147 26.042376 140-149
ACTAT 3789560 1.8985772 17.529932 130-139
GCACA 6101255 1.8978214 18.723862 130-139
CTGTA 4577050 1.8966417 24.431046 190-199
CAGTT 4575265 1.8959022 26.017426 190-199
CTATT 3521975 1.8875127 20.721073 110-119
ACCCT 5453100 1.8842919 44.739674 10-14
GTCAC 5651720 1.8805325 38.792683 160-169
GCTGA 5859545 1.8774124 28.046114 200-209
TGGGA 6059930 1.8696438 16.95041 100-109
TGTCT 4212280 1.8671578 52.42581 30-34
CCTGC 6529790 1.8662255 50.23875 20-24
GCCAG 7214535 1.856114 35.01906 70-79
TGGGG 7223140 1.8432214 120.17874 4
GATGG 5971485 1.8423563 21.151436 100-109
CCAGA 5907085 1.8374238 33.368782 170-179
AGAGA 5237005 1.8262067 27.854977 170-179
AGGAC 6067205 1.8172737 20.507265 220-229
CACAG 5798800 1.8037413 16.146969 140-149
TCAGC 5417720 1.8026721 11.449392 45-49
TACAA 3841460 1.7991679 19.052917 130-139
CAAGG 6004165 1.7983913 17.581188 80-89
TTCCA 4177795 1.797841 22.940908 180-189
TAGTG 4468075 1.7828535 26.732748 120-129
AGTCA 4590140 1.7781217 29.917742 160-169
AGTTC 4288540 1.7770889 23.307802 190-199
ATACT 3545780 1.7764429 26.821674 130-139
GAGGA 6136375 1.7698605 26.870546 220-229
ACCTG 5300610 1.7637055 31.64541 25-29
GCCGA 6823660 1.7555518 19.004637 220-229
CTGAA 4485125 1.7374411 24.45728 200-209
TGACC 5221150 1.7372664 15.118089 210-219
GGAGC 6993880 1.7326467 13.180626 200-209
AGCTA 4468155 1.7308674 26.458704 50-59
TAGTA 3552730 1.7139461 16.825989 120-129
TTCTG 3866095 1.713706 43.55398 30-34
ATGCA 4422255 1.7130866 17.080938 60-69
GCAAA 4720990 1.7096395 32.000042 200-209
CATGA 4396470 1.7030979 23.102606 60-69
CCTGG 6167710 1.6974007 32.578102 2
CTCAG 5100575 1.6971465 77.87052 6
ATTGG 4247365 1.6947856 22.05653 100-109
GTGCA 5266830 1.6875049 49.985302 25-29
GGATG 5366175 1.6556027 22.440025 100-109
AACAC 4324880 1.6264846 22.378622 190-199
GCCGT 5907615 1.6258205 30.056334 230-239
CTCCC 5429130 1.6113863 14.596002 190-199
CAGCT 4827785 1.6063794 13.175922 50-59
GCACT 4796265 1.5958917 27.092272 25-29
GTTCT 3585700 1.5894166 24.662275 190-199
AGGGG 6656740 1.5879942 32.70598 90-99
TCTCA 3668305 1.5785909 26.450611 100-109
GGGCC 7395105 1.5736238 28.080889 2
TTCTC 3416655 1.5727853 23.395813 190-199
TCTGC 4415095 1.5714638 32.707287 200-209
AGTTA 3248680 1.5672631 16.869196 50-59
CGTCC 5482245 1.5668354 19.815449 180-189
CGATT 3768045 1.5614058 34.670364 160-169
GGGGC 7613775 1.5600986 28.279915 90-99
TCTGA 3764795 1.560059 21.444777 220-229
CATCA 3827970 1.5399568 33.40441 45-49
ACAAT 3282260 1.5372635 23.062584 180-189
GGCTT 4475800 1.5340185 21.690296 25-29
GAGCA 5095800 1.5263145 7.8747196 200-209
CCATG 4551705 1.5145175 15.732934 160-169
CGGCC 6847045 1.5130881 31.796377 230-239
GTTAC 3649800 1.5124072 17.16664 50-59
AGTGA 4023545 1.5008591 86.54144 9
CTTTA 2735270 1.4658982 42.23692 45-49
CTGGT 4266450 1.4622663 33.133896 35-39
TTAGT 2832770 1.4618756 25.460028 110-119
GTCCG 5288035 1.4553074 33.584267 70-79
GTGAC 4526930 1.4504392 16.1325 210-219
CTCAA 3589485 1.4440165 19.184893 150-159
CAACC 4466115 1.4426816 15.840131 140-149
GTCCA 4318295 1.4368535 30.0034 180-189
ACTCC 4118855 1.4232501 16.886242 140-149
GGCAG 5744570 1.4231458 13.679156 150-159
AAGCT 3650635 1.4141777 17.896864 200-209
AACCC 4375325 1.413354 21.139912 140-149
CGTGT 4107845 1.4079071 24.884348 230-239
TAGCA 3630660 1.4064398 12.088359 130-139
AAGAG 4013950 1.3997127 20.35676 150-159
TACGC 4194930 1.3958057 24.198961 140-149
CAACT 3463425 1.3933038 18.847816 130-139
AGCAG 4598930 1.3774902 12.878318 50-59
ATCTA 2728795 1.3671318 21.847706 110-119
CACGT 4096025 1.3628962 17.212011 180-189
ACAGT 3511205 1.3601655 13.196773 120-129
GGCCT 4935355 1.3582473 68.5749 3
GGGGT 5310235 1.3550808 114.53946 7
TCAAG 3468320 1.3435527 24.425371 150-159
GAGAG 4640165 1.3383218 25.565413 220-229
CCATA 3322395 1.3365687 15.984195 160-169
CGACA 4293440 1.3354928 16.412687 70-79
TTACA 2664985 1.3351629 10.540566 120-129
TGTCC 3747850 1.3339715 56.208073 15-19
TGGAA 3565035 1.3298262 19.929466 120-129
CTACG 3989815 1.3275564 16.11616 130-139
GTGGC 5005085 1.3263783 15.016251 40-44
AACCA 3520795 1.3240875 18.062103 190-199
GTGCG 4988300 1.3219302 17.652874 240-247
GAACT 3412260 1.3218362 14.428296 190-199
CAATT 2621225 1.313239 24.330906 180-189
CCAGT 3939855 1.3109329 19.726118 190-199
ACAGA 3582130 1.2972174 20.427448 140-149
ACATG 3346690 1.2964358 14.58336 190-199
ATACA 2768030 1.2964212 22.499022 35-39
CCTTT 2810040 1.2935427 48.634247 45-49
TGCGA 4036060 1.2931632 14.78926 240-247
TGTGT 3026455 1.2917948 15.1883335 240-247
GATTG 3229070 1.288465 25.21677 100-109
ATCCG 3867025 1.2866998 24.848402 70-79
AGAGC 4282870 1.2828225 24.628635 220-229
AGACC 4112860 1.2793225 103.820564 9
AATTC 2548245 1.2766759 24.322432 180-189
ACGTC 3831375 1.2748376 19.090858 180-189
TCCGT 3528405 1.2558645 13.617511 150-159
TCACT 2915965 1.2548345 12.971831 190-199
AGTCG 3889380 1.2461667 17.120153 150-159
ACATA 2659905 1.2457802 21.050425 130-139
TTATT 1865695 1.2452132 19.395363 240-247
ATATT 1988365 1.2406093 26.191223 240-247
AAAAA 2433715 1.2405556 7.746704 190-199
CCTAC 3570330 1.2337099 17.115881 190-199
TCGGA 3820685 1.2241565 98.33998 5
ATCAT 2440455 1.2226729 16.20443 110-119
CTTCT 2651430 1.22053 23.721712 30-34
CGGCT 4399610 1.2108063 20.791267 230-239
ATTAT 1940275 1.2106043 9.602782 50-59
GATCC 3627360 1.2069545 24.653028 70-79
GTCGA 3749070 1.2012109 13.310876 150-159
TGGAC 3740535 1.1984763 16.192831 80-89
CTTCG 3351375 1.1928542 110.08549 3
TACCA 2961220 1.1912714 10.833559 45-49
ACACA 3153250 1.1858624 7.659467 130-139
TGAGT 2967945 1.1842709 21.231413 90-99
AGCTC 3511015 1.1682423 12.857608 210-219
GAAGT 3130700 1.1678109 13.863639 140-149
ATTAG 2411345 1.163307 25.084627 110-119
GATCT 2806070 1.1627817 9.567715 220-229
TTGGG 3519110 1.1614171 16.354681 100-109
AAGGC 3872065 1.1597764 29.291174 25-29
GGGTG 4543095 1.15932 14.699606 60-69
TCGAG 3617600 1.1590875 13.029366 150-159
GGACT 3595385 1.1519699 13.858964 90-99
CGCTG 4182740 1.151122 12.394581 190-199
ACGCA 3677605 1.1439346 20.8345 140-149
GTATA 2365845 1.1413563 10.372784 240-247
TTCGG 3327510 1.140458 106.210526 4
GCCCC 4967825 1.1400707 12.548367 80-89
ATTCC 2649040 1.139968 22.428873 180-189
GAACC 3663935 1.1396825 11.2478695 180-189
GACCC 4262490 1.1388439 44.016766 10-14
AGGCC 4391835 1.129906 14.366094 2
GCAAG 3744485 1.1215633 17.091017 25-29
GAAGC 3742545 1.1209822 13.904413 200-209
ACAAG 3094265 1.1205438 17.86816 80-89
AGGTC 3481885 1.1156043 18.356106 10-14
CCCCT 3757175 1.1151438 10.297124 140-149
TAGAC 2868395 1.1111547 18.649904 170-179
AGATC 2862570 1.1088983 12.8508215 210-219
GCACC 4123205 1.1016301 11.4193 130-139
ATCAA 2344920 1.0982555 13.3582325 110-119
CTTGA 2636930 1.0926933 22.497812 90-99
TTGAG 2730420 1.0894935 21.024828 90-99
GCTTC 3058565 1.0886345 28.601913 2
CGCAG 4207310 1.0824325 16.277916 140-149
TACAG 2775945 1.0753416 9.729813 120-129
TGGCT 3134075 1.0741608 33.19335 40-44
GGAGA 3715910 1.0717471 88.20744 7
CCGAT 3210535 1.0682616 23.062954 160-169
GCTCT 2991350 1.0647107 18.198534 210-219
CTATA 2124040 1.0641483 13.82855 50-59
ATGGG 3441895 1.0619129 19.911043 100-109
CAGCA 3405685 1.0593528 12.558428 45-49
CACAT 2631205 1.0585093 13.922642 130-139
GTAGA 2799760 1.044364 18.276222 170-179
ATGGT 2599120 1.0371021 10.02749 35-39
TAGTT 2008200 1.036349 15.623966 50-59
GGGAG 4343710 1.0362109 14.892265 5
ACACC 3185585 1.0290341 13.216765 40-44
CTCCG 3599125 1.0286363 9.701663 140-149
ACCAA 2731910 1.0274065 18.111076 130-139
ATGAG 2753165 1.0269831 20.100195 60-69
GTAGC 3194680 1.0235832 11.407042 50-59
ACCGC 3828335 1.0228472 11.197152 220-229
GAGCC 3960515 1.0189385 17.668486 220-229
GACAG 3394615 1.0167688 15.842366 70-79
CGAGT 3143205 1.0070904 15.453525 160-169
CGTGA 3137720 1.0053331 20.409851 150-159
AAACT 2135280 1.0000695 17.44471 130-139
CCAAC 3078480 0.99443614 14.999804 130-139
TGAGG 3216630 0.99241287 11.963608 220-229
TTTCA 1845265 0.9889227 6.94463 100-109
TTATA 1581215 0.9865744 13.832219 110-119
GGTAT 2461960 0.98237246 9.704334 110-119
AAGGT 2628445 0.9804602 20.87554 15-19
AGCCG 3804335 0.9787575 18.640007 220-229
ATGAC 2522710 0.97724366 11.018351 160-169
GGGAC 3940040 0.97609586 10.620606 90-99
ATGAT 2012535 0.97090876 9.341761 120-129
CGAGG 3917000 0.970388 14.31744 220-229
TTTAG 1865210 0.96255785 30.13885 45-49
AGCCC 3589075 0.9589222 11.754319 80-89
AACTC 2379105 0.9570919 15.909483 190-199
ACTCA 2369815 0.9533546 11.89826 190-199
CAGAA 2623340 0.95000523 18.515747 140-149
CGGAC 3690575 0.9494899 11.183921 220-229
CCGTC 3320390 0.9489734 10.304912 140-149
GCCAA 3032225 0.9431864 14.420508 180-189
CCCCA 3396870 0.9425062 17.558506 80-89
GATAC 2432490 0.9422944 24.390095 35-39
AATCA 2008085 0.94049704 14.673547 110-119
CCCAG 3515090 0.939155 15.491455 80-89
GCAGT 2927060 0.937837 6.370433 50-59
TATGG 2339765 0.93361413 13.015142 35-39
ACAGG 3112370 0.9322294 15.360542 70-79
CCGAG 3606545 0.92787117 17.50745 220-229
GACTG 2890790 0.9262161 13.491227 90-99
TGGTT 2165080 0.9241304 9.733493 50-59
GCCAT 2777135 0.92405367 7.7823744 60-69
CATAC 2292730 0.922344 14.161 130-139
AAGTT 1891990 0.9127541 17.98514 140-149
TACAC 2263345 0.9105228 22.133875 35-39
TAACA 1939020 0.9081501 8.513746 120-129
GAGAT 2425730 0.9048436 12.240354 210-219
TCATA 1802450 0.90303105 8.927716 110-119
AACTG 2317630 0.8978001 11.204737 60-69
TCCTT 1945740 0.8956805 11.292177 1
CGGAG 3599100 0.89163226 76.00379 6
GTTAT 1711170 0.88306415 11.146011 110-119
AGAAG 2526270 0.8809408 16.204899 140-149
TTTAT 1315310 0.87787193 9.840536 240-247
CGTAT 2115485 0.8766165 7.612762 240-247
GGTAG 2802365 0.8646015 12.279659 120-129
ATAGT 1774485 0.8560661 16.016949 120-129
TTACC 1973515 0.84926766 9.830982 45-49
ATATG 1759300 0.8487404 6.3124957 50-59
GATCA 2187850 0.84752613 9.449756 110-119
GCCTA 2541160 0.8455363 16.557184 190-199
CATGG 2630590 0.8428472 8.776831 200-209
GTCTA 2030720 0.84149146 10.408839 30-34
ACGCT 2527990 0.8411541 15.279584 190-199
GATGA 2248850 0.838864 6.4711037 60-69
ATCCA 2084825 0.83870584 9.734843 180-189
TATTT 1249085 0.8336716 10.009263 240-247
TCTTC 1802275 0.8296394 48.465546 2
GCTTG 2413715 0.8272674 17.25572 90-99
TGGCA 2574760 0.8249593 5.914845 100-109
CTATC 1907530 0.8208721 8.165806 110-119
AGTCT 1980415 0.82064605 15.482785 8
GGTTC 2371835 0.8129136 11.180656 160-169
CCTCC 2735575 0.811929 11.286985 140-149
GGTGC 3063345 0.8118053 11.710265 60-69
ACATC 2015520 0.81082505 6.197203 110-119
GTCTG 2351175 0.8058325 13.741027 3
TCATC 1857795 0.7994696 10.328273 110-119
ACAAA 1819085 0.7964604 12.917361 130-139
CATTA 1569130 0.7861373 11.95522 110-119
ATATA 1336790 0.77971876 10.401533 130-139
TGATG 1949010 0.7776949 11.946275 120-129
TTATG 1490190 0.7690255 11.041529 50-59
TATAC 1529685 0.7663753 8.692674 130-139
GGTTA 1920415 0.76628494 8.670028 50-59
CCAGC 2835455 0.7575714 6.2801914 80-89
TGACT 1814125 0.75173867 5.0303884 2
CTGAC 2257290 0.75108236 9.656571 220-229
CCCCC 3142250 0.7488774 13.80733 80-89
GCGGA 3019800 0.74811786 9.520074 220-229
TCAAC 1854640 0.7461045 8.092425 110-119
CATCC 2148005 0.74223256 6.6815996 110-119
TTGGA 1858875 0.74172926 6.486254 60-69
GTTCA 1785300 0.7397941 13.593513 160-169
GGGAT 2389255 0.73714644 13.354093 100-109
GACCG 2857040 0.73504287 9.911996 210-219
GATTA 1508085 0.7275466 5.901793 50-59
TCCGG 2638150 0.72603893 12.51334 70-79
ATCTT 1349055 0.72299165 13.560058 3
CGCCG 3268060 0.72218925 11.229894 220-229
ATAAA 1313835 0.71639323 8.225472 130-139
ATGCC 2126365 0.70751894 8.12804 50-59
TTTAC 1319385 0.70709074 13.300616 45-49
GGGCA 2849350 0.705891 13.655386 150-159
TGCCA 2115010 0.70374066 7.4812613 60-69
AACGC 2257370 0.7021645 14.15537 180-189
GGATA 1878420 0.7006866 15.780613 35-39
GACCA 2249890 0.69983786 6.3255205 170-179
CACAA 1850740 0.69601935 8.515148 130-139
TATTG 1347995 0.69564456 6.335494 240-247
ACGCC 2592410 0.6926351 12.561391 180-189
CAAAC 1816705 0.6832196 13.903961 130-139
GAAAT 1514115 0.6828569 7.6204557 110-119
CAACG 2185895 0.6799319 14.422621 180-189
AATAC 1445430 0.67697465 11.410953 130-139
GCCGC 3050050 0.6740125 12.357794 220-229
TATAG 1388235 0.66972727 6.1612496 120-129
AAATA 1222850 0.66678196 12.630508 130-139
TTTCT 1161775 0.66602385 9.8391075 4
AGGCA 2202170 0.6596028 6.692738 80-89
CCCGT 2272870 0.6495903 10.053314 140-149
AATAA 1190600 0.64919704 7.6643724 130-139
CAGTC 1948010 0.6481737 5.3041706 210-219
GCGAC 2514845 0.64700484 7.2570133 70-79
CCGGC 2925900 0.6465773 10.137226 70-79
CACGC 2418365 0.6461341 12.312233 190-199
TCAAT 1266210 0.6343737 8.873149 110-119
GACGA 2112385 0.63271004 9.845833 220-229
ACTTC 1466185 0.63094705 30.207829 4
TACTT 1174110 0.6292344 11.253845 1
TCCAC 1810250 0.625523 10.373101 180-189
CCCTA 1777250 0.61412 12.101937 110-119
AGATG 1638125 0.6110519 5.535193 120-129
CACCG 2286260 0.61083853 9.581872 45-49
TAGCT 1472625 0.6102276 12.653001 50-59
AAGCA 1684350 0.60996336 5.995133 120-129
AGTAA 1348190 0.60802567 5.1868653 50-59
GGCCC 2746070 0.6068378 10.460391 70-79
CACGA 1943565 0.6045541 8.921193 180-189
CTTTG 1332855 0.59080845 7.3707924 45-49
TGATT 1138435 0.58749926 6.371123 50-59
AATGG 1574935 0.58748084 6.6284294 120-129
CCGTA 1740440 0.57910764 6.5196333 230-239
CGGCA 2241260 0.5766185 11.6765785 70-79
ACGAC 1853660 0.5765888 9.189493 220-229
ACCCG 2157115 0.5763338 8.816322 140-149
ACCTA 1425125 0.57331455 7.098657 130-139
TCAGG 1787300 0.57265514 5.7046328 150-159
ATTAA 981480 0.5724746 6.188704 110-119
GGGGA 2386495 0.5693088 9.081983 4
GTTCC 1594510 0.5675336 6.595503 140-149
GGTGA 1834110 0.56587 6.145316 120-129
TTAGC 1352460 0.5604335 9.6189 45-49
GAAGA 1604580 0.5595364 6.2481804 15-19
TAATA 956345 0.55781394 8.370912 130-139
CTGCG 2012855 0.553953 12.212712 25-29
ATTTC 1023770 0.54866344 5.95295 240-247
CGGTT 1592055 0.54565483 11.1101465 160-169
ACCCC 1953205 0.54194236 9.675264 140-149
GCTAC 1623820 0.54030395 8.229764 50-59
AAATC 1150435 0.5388122 8.188355 110-119
GTCCT 1500070 0.53391963 6.5842085 45-49
TTCAA 1063900 0.533016 5.246522 200-209
CCTAT 1230335 0.52945316 5.1585035 110-119
CCACG 1980715 0.5292036 7.780581 180-189
GGAGG 2207860 0.5266946 5.9647427 6
GGGTA 1685920 0.52014965 5.6665974 100-109
AAGAT 1147465 0.5174999 5.610135 15-19
AAGTA 1123985 0.50691056 5.108044 120-129
CTGTT 1139455 0.50508094 5.7700663 240-247
AGTTT 975925 0.5036346 5.2219706 140-149
TATGT 972140 0.5016813 6.3476143 140-149
CGCGG 2294540 0.4882611 6.6686797 220-229
TGACG 1519535 0.48686266 8.825094 220-229
GTAAC 1247085 0.48309395 6.740451 120-129
GGGTT 1444745 0.47681138 5.478963 100-109
ACATT 941240 0.47156313 5.8677945 110-119
TAAAT 806785 0.47057906 8.187384 130-139
TATCC 1085295 0.4670377 5.408266 110-119
ATGCT 1108165 0.45920235 7.297003 50-59
TGGCC 1660505 0.4569836 14.454758 2
CAATC 1131820 0.45532072 6.633626 110-119
TGCTA 1091795 0.45241892 6.43985 50-59
CCGCG 2046395 0.4522207 6.889243 220-229
CCGGT 1639350 0.45116156 8.563046 160-169
AACAT 953655 0.44664925 5.5857778 110-119
GGTTT 1043420 0.44536746 5.1389956 100-109
TGCGC 1580755 0.43503574 9.091159 25-29
TTGGT 1017470 0.43429115 5.21769 120-129
CATAA 918495 0.4301819 5.7433815 110-119
GGTCA 1337270 0.4284645 5.4896283 160-169
ATTCT 793145 0.42506582 8.216409 2
GCCTT 1191745 0.4241776 8.96291 1
GCGCT 1527015 0.42024615 8.275769 25-29
GCATG 1291465 0.41378853 8.271692 60-69
CTAAC 1011745 0.40701565 5.588 110-119
ACGAG 1352225 0.4050238 7.8425794 180-189
CCTAA 992035 0.39908648 7.7748322 110-119
CACTT 924710 0.39793277 6.967948 3
ATGGC 1232135 0.394779 6.911758 1
CCGGG 1831900 0.38981473 5.1680512 80-89
CATGC 1169545 0.38915008 7.083394 60-69
TTTGA 753260 0.38872638 6.217458 45-49
AGCTT 933325 0.38675204 7.2631564 1
TACCT 893205 0.38437515 5.2123404 2
GGTAA 1017565 0.3795712 7.075518 120-129
GAGGC 1474780 0.36535838 5.8735156 35-39
TTCTT 628075 0.3600636 20.151167 1
TGGGC 1316380 0.34884882 9.449667 1
CGAGC 1316860 0.33879417 6.584831 180-189
TGCTG 984645 0.33747336 14.182072 1
AACCT 784510 0.31560114 5.371585 2
CTCTT 664920 0.30608192 9.453176 1
AACTT 604985 0.3030987 10.931829 3
CTCGG 1100405 0.30283982 5.322783 6
GCGAG 1205375 0.29861665 5.3851223 240-247
CGCTT 814585 0.2899351 6.2637043 1
GACTT 689085 0.28554365 6.8306127 1
TCACA 707320 0.28454828 5.5573745 7
TTCCT 615335 0.28325653 6.3123493 4
GAGGT 890295 0.27467886 7.47797 7
ATTGT 523700 0.27025995 5.21658 240-247
TAACT 537940 0.26950902 8.137704 2
CCCTT 706510 0.26114827 5.9311967 3
GTCTT 570195 0.25274768 12.921499 1
TGTAA 513955 0.2479477 7.6949954 25-29
TTGGC 719675 0.24665864 7.1344333 1
CGGGG 1191415 0.24412663 8.981755 3
AATCT 479360 0.24016032 7.5275307 2
CCTCG 826505 0.2362166 5.780743 5
TCGGT 668030 0.22895798 6.342245 7
TGCTT 498940 0.22116281 11.490158 1
TAGGC 495140 0.15864404 6.8199334 1
TCGCA 470855 0.15667056 5.5172305 7
TTGCT 268525 0.11902784 5.6244426 4
Produced by FastQC (version 0.10.1)