FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_17_SL248346.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_17_SL248346.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6727053
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7680500 8.337356 58.925667 110-119
CACAG 10180250 7.250908 41.078346 110-119
ATACA 7022610 7.2255955 35.46899 110-119
TCCCT 11938725 7.0575542 106.30028 30-34
CAGTA 8628995 6.484223 38.37639 110-119
AGTAA 6667520 6.1354 45.977367 110-119
GGAGA 10573445 6.0236177 91.9113 35-39
CCCTG 11942715 5.984654 69.93018 30-34
GGTGA 11829070 5.805964 101.24111 45-49
GTAAT 7256230 5.7527094 38.65973 110-119
AATAC 5085975 5.2329836 37.645496 110-119
GTAGT 8987790 5.2039948 27.821507 80-89
AGACG 8031450 5.11601 98.558716 40-44
ACAGT 6718350 5.0484767 34.786148 110-119
GTGAC 9062250 4.9734335 83.317635 45-49
CCCCA 7234050 4.7046914 100.618835 60-69
GAGAC 7382390 4.7025604 137.90578 40-44
ACTCC 6805980 4.669862 119.647606 30-34
CTCAG 7511865 4.609615 34.41989 140-149
ACCAG 6346860 4.5205665 92.09169 45-49
GGCCC 10439310 4.434529 59.683735 60-69
TAGTA 5579865 4.4236946 34.954712 80-89
GCACA 6086235 4.3349357 33.496796 110-119
GTGTC 9157860 4.3300915 23.653915 120-129
CGTGT 9095805 4.3007503 23.169949 120-129
CACTC 6198045 4.2527328 97.24409 25-29
TGACC 6907340 4.238651 142.24857 45-49
CTCCC 7431445 4.163953 69.2852 30-34
AGTAG 6102325 4.1010633 28.29495 80-89
GACCA 5533135 3.940989 104.155426 45-49
CCCAG 6768625 3.936893 85.88054 60-69
ATGCA 5217110 3.9203758 93.02629 25-29
AAAAA 2623420 3.8365514 25.275278 130-139
TGAGG 7621000 3.7405517 68.0037 35-39
CCACT 5304405 3.6395698 26.659624 240-247
ATGGT 6281550 3.637063 25.369598 190-199
AGGAG 6383605 3.6366951 92.237816 35-39
CAGGG 7808155 3.6325233 35.555546 50-59
ACGGT 6602670 3.6235967 94.819664 40-44
GACGG 7779575 3.6192274 106.41658 40-44
TACAC 4298660 3.6118302 26.117928 110-119
CTGAG 6557175 3.5986288 104.16567 35-39
TGTCC 6782480 3.5858173 20.50087 120-129
CCCTT 6054445 3.5790734 35.220486 20-24
TGCAC 5696910 3.4958773 113.71908 25-29
AGATA 3788675 3.4863095 18.810322 150-159
GGGTT 8168975 3.454412 42.635017 50-59
GCCCC 7269405 3.4527972 73.50477 60-69
GAGGA 6002065 3.4193337 120.81608 35-39
TGGTG 8061730 3.4090612 17.995508 190-199
CCTGA 5440125 3.3383029 83.34093 30-34
CCGTG 7368160 3.302163 23.518663 120-129
GGATG 6704875 3.2908978 68.99589 20-24
CCAGG 6312005 3.283401 40.350506 50-59
TCTTG 5881490 3.2805812 17.863064 170-179
CTTCA 4526280 3.2765634 16.886084 200-209
CTTGG 6926820 3.2751944 20.039188 20-24
AGGCT 5954985 3.268142 24.394331 30-34
CTCTC 5514360 3.2598033 13.679077 130-139
GCACT 5276150 3.2376802 88.207985 25-29
CCTGG 7112140 3.1874232 29.812246 50-59
CAGAT 4240155 3.186247 17.613447 150-159
TCAGG 5804870 3.1857579 20.740969 140-149
TCGCA 5078370 3.116314 23.630383 100-109
TGTCT 5572715 3.108352 19.624098 180-189
TCTCA 4240245 3.0695033 21.396646 130-139
CGCAC 5230080 3.0420153 24.149126 100-109
GGTTG 7143845 3.0209155 74.066185 15-19
TTCCC 5106085 3.0184517 49.96863 50-59
CGGAT 5454205 2.9933102 78.35005 20-24
GGTTC 6209350 2.9359539 48.12487 50-59
ACAGA 3358555 2.9293234 13.709668 140-149
TGGCC 6533050 2.9278939 43.89598 60-69
GATGC 5277480 2.896322 95.45154 25-29
ACCAC 3628790 2.8899636 19.312809 230-239
AGGGT 5876605 2.8843648 49.70151 50-59
GCCCT 5721255 2.866997 19.060995 20-24
TCTCT 4546965 2.835839 15.233003 180-189
GTTGG 6663380 2.8177414 52.76641 15-19
GACTC 4567455 2.8027935 18.936563 190-199
GCCGT 6195925 2.7768059 22.286652 120-129
CGGTG 6916055 2.772051 68.0056 40-44
CCAGT 4462795 2.7385697 36.65414 60-69
AGTCA 3643500 2.7378929 31.419828 70-79
CGGCC 6434335 2.7332497 12.721502 120-129
TCAAA 2645615 2.7220857 55.981945 70-79
GATGG 5538500 2.7184157 19.376295 15-19
TTCTT 4102875 2.6996782 20.118086 170-179
GTTCC 5056980 2.6735659 54.48468 50-59
AGATG 3836290 2.5781763 16.648321 180-189
TCACA 3043880 2.5575361 11.029545 140-149
GGCTG 6316995 2.53194 12.831635 30-34
TCCAT 3481990 2.5206044 17.15047 70-79
GAGAT 3726375 2.5043077 16.301102 180-189
TCCTC 4227520 2.4990902 17.196957 130-139
TGTTC 4471155 2.4939232 13.05667 140-149
GCGGA 5342305 2.4853563 85.99728 20-24
TGGAG 5010880 2.4594486 16.531973 25-29
AGGGA 4301970 2.4508016 8.698129 150-159
GGCGG 7169695 2.4360335 42.238873 20-24
ACAGC 3363460 2.395633 10.951119 120-129
ACTCT 3279740 2.3741958 13.126389 200-209
TTGGG 5590995 2.3642626 79.376305 15-19
CTGGC 5242315 2.3494298 35.244934 60-69
AATAA 1863310 2.347692 9.634157 110-119
TACCA 2789955 2.3441825 15.935793 230-239
GTTCT 4188515 2.336272 17.366707 160-169
CACGG 4457955 2.3189545 9.46202 120-129
CTGGA 4204435 2.3074267 14.505049 180-189
CAGCT 3749220 2.300688 11.982265 150-159
TGTAG 3895455 2.2554967 7.6893435 210-219
CATTT 2942635 2.2473838 17.64903 150-159
TCATT 2938250 2.2440348 16.515543 150-159
TTCAC 3070030 2.2223873 18.641232 200-209
TTCAT 2894290 2.2104611 15.937951 140-149
TCAGC 3599825 2.209013 13.990619 140-149
ACTAC 2611995 2.1946566 12.196853 230-239
TCTGC 4128245 2.182555 12.264837 210-219
GTGAA 3228040 2.1694021 17.663864 190-199
TTGTC 3882445 2.1655524 13.169477 170-179
CTACT 2985430 2.1611454 7.3681245 230-239
CAGAG 3372145 2.1480465 9.021306 140-149
CACTA 2535600 2.1304677 14.832619 240-247
GATAC 2813150 2.11393 14.273903 150-159
ATTTG 3088125 2.109304 15.4908905 150-159
TGGGG 5874955 2.1059635 52.64318 15-19
CTCGC 4152525 2.080886 16.251322 100-109
GCTGT 4396790 2.078925 10.244344 140-149
TCTCG 3928750 2.0770843 16.983816 100-109
CTCTG 3922180 2.0736108 14.637294 180-189
ATCTC 2854725 2.0665286 9.21448 100-109
TAGTC 3181410 2.0596821 26.18124 70-79
GTCCT 3894630 2.059045 14.580594 130-139
GAACT 2739800 2.0588114 10.344811 160-169
TTGGA 3550085 2.055525 15.618185 170-179
GTCTC 3885115 2.0540147 11.960119 180-189
ACACA 2080230 2.028723 13.192009 110-119
GAATC 2682360 2.0156484 14.223504 190-199
TACAG 2681630 2.0150995 13.414383 160-169
ATATA 1855010 2.013655 10.391527 110-119
GTTCA 3098470 2.0059855 14.895352 140-149
CAGGC 3846540 2.0009065 15.448547 140-149
ATGTA 2511225 1.9908891 7.4408526 70-79
AGCTC 3243270 1.9902147 12.00944 140-149
GATAT 2502500 1.9839717 7.785358 180-189
ACACG 2769005 1.9722306 12.950435 120-129
GTCAA 2615300 1.9652562 39.68926 70-79
TGAAT 2469655 1.9579324 13.527573 190-199
TTGCA 3014820 1.9518298 14.176598 150-159
TTTGC 3498830 1.9515795 12.172585 150-159
CCTTC 3295785 1.9482969 14.142787 200-209
AATAT 1792385 1.9456745 12.997185 110-119
AATCG 2581165 1.9396058 13.070225 190-199
AACCA 1988595 1.939357 14.766235 70-79
ACGGC 3727160 1.9388071 14.270015 120-129
TGGAA 2864870 1.9253341 9.262633 170-179
GAGTC 3483715 1.9118898 12.165036 210-219
TCGGC 4215355 1.8891809 7.9642177 130-139
CGTAG 3439685 1.8877257 9.7443495 210-219
TACTA 2118345 1.8778229 9.643647 230-239
GGGGC 5525575 1.877414 48.05529 15-19
CAGCC 3213060 1.8688391 10.193464 120-129
ATATG 2336015 1.8519832 8.0394 180-189
CATAG 2442995 1.8357786 8.000325 210-219
GGGCG 5381915 1.8286029 59.575752 20-24
CTGTT 3221735 1.7970212 12.874696 140-149
AGTAT 2250120 1.783886 11.537182 220-229
CCAGA 2490890 1.7741429 25.889462 60-69
GTTGT 3554300 1.7730497 6.848825 210-219
GACGT 3228360 1.7717491 9.388173 170-179
CTCCA 2555705 1.7535741 7.8449144 150-159
GTCAC 2812200 1.725691 8.26269 130-139
CACCA 2165070 1.7242589 13.291847 230-239
ATTGT 2523210 1.723446 7.50749 170-179
AGTCT 2660390 1.7223674 13.008856 210-219
TGCAG 3118170 1.7112758 13.43886 150-159
GCAGA 2679900 1.7070885 14.283265 150-159
AGAAC 1956825 1.706738 10.7787485 160-169
CCTTG 3190605 1.6868356 22.802578 20-24
GTCTG 3529980 1.669073 9.110988 210-219
CAAAA 1393085 1.6636802 20.313374 70-79
TGACT 2566370 1.6614978 11.504858 190-199
GGTCA 3021490 1.6582171 7.399629 130-139
ATAGT 2080520 1.6494278 7.2672987 220-229
TTCAG 2534295 1.6407324 9.090274 150-159
TGGTT 3278100 1.6352683 17.9683 10-14
TCAGA 2164695 1.6266512 9.960524 130-139
CTGTG 3418415 1.616322 5.3245277 210-219
TGTGC 3415420 1.6149058 8.192723 220-229
TCCCA 2350605 1.6128466 10.453931 240-247
AACTG 2136130 1.6051861 10.27373 160-169
GTCCC 3186910 1.597003 20.24339 50-59
TCTGG 3373580 1.595123 12.044399 180-189
TGCTC 3016185 1.5946215 5.444359 150-159
GCTCC 3174235 1.5906514 6.432735 150-159
TTGGT 3178750 1.585708 24.244486 25-29
GAGAA 2017365 1.5736305 9.85209 160-169
GTGCT 3321055 1.5702873 7.9487853 220-229
GAACC 2195170 1.5635152 12.386251 70-79
CTGGT 3303045 1.5617719 6.883479 160-169
AATAG 1697130 1.5616859 9.026782 240-247
GGCCG 4102435 1.5585505 13.10957 120-129
TAGTT 2276760 1.5551115 9.641674 220-229
TTGTA 2270575 1.5508869 8.589112 210-219
ACTGA 2059805 1.547832 8.356663 180-189
CAAAG 1763835 1.5384126 28.135775 70-79
ATAGA 1659925 1.52745 9.523124 240-247
GCTCA 2476485 1.5196813 10.854985 140-149
GGACG 3255740 1.514641 7.067669 170-179
AGATC 2008110 1.508986 6.879601 130-139
CCACC 2316090 1.5062778 9.683307 230-239
ACCAT 1780435 1.4959613 11.288955 45-49
AAATA 1181390 1.4885018 5.106101 9
GCTCT 2804520 1.4827167 10.086453 130-139
CCTCG 2947785 1.4771745 9.758886 130-139
CCCAC 2265690 1.4735 9.971832 240-247
CAGAC 2037910 1.4515066 15.306937 60-69
CATGT 2193035 1.4197967 7.570258 70-79
GCTGA 2579560 1.4156826 20.8858 30-34
CGACT 2300535 1.4117106 8.310614 190-199
CTGCT 2653900 1.4030858 5.828563 90-99
TATAC 1575385 1.3965122 6.6812325 110-119
CACTG 2272285 1.3943751 6.9778895 230-239
GCGTA 2531240 1.3891641 10.1424885 210-219
CTCTT 2222270 1.3859795 7.966261 200-209
AATTG 1744235 1.3828225 9.169324 170-179
TCGAC 2249680 1.3805038 9.012354 190-199
TGGAC 2498545 1.371221 10.078696 170-179
GAGGG 3263965 1.3580285 7.887203 200-209
CCTCA 1977665 1.3569572 7.43404 130-139
TGATA 1711385 1.356779 7.377617 180-189
CTTGA 2082375 1.3481541 8.045018 200-209
GGGAG 3226060 1.3422577 6.3209558 150-159
GATCT 2071495 1.34111 5.426687 130-139
ACGGA 2092895 1.333168 9.232494 200-209
CCATC 1930250 1.3244237 10.614722 240-247
ACTTC 1828245 1.3234621 6.5804873 220-229
GTGGA 2674345 1.3126265 21.161865 25-29
GTAGG 2673430 1.3121773 5.1363597 160-169
GGAAT 1925270 1.2938765 8.6053505 170-179
ATCGG 2354215 1.2920114 7.724808 190-199
CTCGA 2105120 1.2917954 8.061168 190-199
GTGTT 2588870 1.2914485 7.646593 160-169
GAATT 1628780 1.2912902 10.287648 170-179
CCATG 2101355 1.2894849 7.665676 70-79
CTGAT 1980500 1.282199 7.1193776 180-189
TCACG 2087815 1.2811761 9.653287 200-209
TATGG 2208260 1.2785985 5.9290175 180-189
TCCAC 1850140 1.269457 9.173525 240-247
ACCGT 2065615 1.2675532 18.1917 45-49
GGAGT 2575870 1.2642928 7.0309644 210-219
GGCTC 2791245 1.2509425 8.429039 130-139
AGCCG 2401750 1.2493507 9.065952 120-129
ACGTG 2265660 1.2434119 9.015091 170-179
ATAAA 986410 1.242835 5.119916 7
GGAGG 2965350 1.2337848 16.061886 30-34
ATAAC 1197615 1.2322316 7.398564 80-89
GTCCA 2005895 1.2309064 16.178362 70-79
ACTAT 1380670 1.2239054 5.9373903 230-239
AAGTA 1329815 1.2236855 15.458666 70-79
AAAGT 1315880 1.2108626 20.772945 70-79
ACTCG 1968845 1.2081708 8.29417 190-199
GTGGT 2856200 1.2078004 10.612796 50-59
AGAGA 1547445 1.207073 12.588212 35-39
TGGTC 2535225 1.1987251 12.019013 50-59
TTGGC 2515685 1.189486 8.189101 60-69
TGCCC 2368930 1.1871023 6.8198056 60-69
ACTGG 2150560 1.1802441 6.983193 160-169
TTGAG 2032010 1.1765486 7.736658 200-209
TTCCA 1615625 1.169547 7.447687 230-239
GCATA 1552300 1.1664695 7.7472844 210-219
AGAGC 1825820 1.163042 6.7461376 140-149
AAAAG 1075880 1.1491047 13.553676 70-79
AGCTT 1760745 1.1399269 5.8916717 150-159
ATACC 1355240 1.1387029 7.240942 70-79
GAAGA 1459505 1.138476 18.323668 35-39
TCTGT 2038630 1.1371083 6.5336833 210-219
TATCA 1274205 1.1295286 6.670867 70-79
ATATC 1270280 1.1260493 7.103463 70-79
ACTGT 1737595 1.1249394 5.3646154 230-239
CAATA 1077850 1.1090049 6.4026637 100-109
CCATA 1306235 1.0975279 10.015928 70-79
CGGCT 2438950 1.0930556 7.9135785 130-139
CTGAA 1452085 1.0911633 14.31857 35-39
AGTTC 1680655 1.0880756 5.2019863 160-169
CTCGG 2427670 1.0880005 8.415978 130-139
TCTAC 1497660 1.0841525 6.8982553 180-189
AGGTT 1866565 1.0807545 22.674465 10-14
GTCTA 1658965 1.0740333 7.043029 180-189
CTTCT 1716190 1.0703489 5.555986 210-219
CTGCG 2377615 1.0655675 6.9441237 210-219
AAAAT 845140 1.0648409 5.1687326 8
GCTTC 2012730 1.0641067 5.3508644 150-159
CTGCC 2112990 1.0588475 5.924278 200-209
TATGT 1546490 1.05631 7.3967795 220-229
TACTG 1622455 1.0503962 6.1946588 180-189
CTACC 1526215 1.0471987 8.442765 230-239
ATCCA 1243585 1.0448879 14.342703 240-247
ATGGG 2117360 1.0392462 30.373503 15-19
GAGCT 1891030 1.0378119 6.1199775 140-149
AACTT 1160765 1.028969 5.7936254 210-219
GGGGT 2863560 1.026485 7.753308 10-14
GCGGT 2551150 1.0225365 5.728555 130-139
TGCGT 2148945 1.0160811 8.21657 210-219
CATGG 1837420 1.0083904 5.233272 190-199
TAGAT 1259630 0.99862957 7.3401394 240-247
ATACG 1314160 0.98752004 5.1646733 120-129
TATAA 906155 0.98365176 6.235267 240-247
AAGAG 1260535 0.983271 13.585071 35-39
CAGCG 1888070 0.9821428 6.3340135 160-169
TCCCC 1740665 0.97532135 5.066901 80-89
CGGAG 2069215 0.9626438 6.637535 210-219
CGAAC 1347655 0.9598706 12.983791 70-79
ACAAT 927265 0.9540673 7.4057403 100-109
AGCTG 1732910 0.9510345 5.128596 90-99
TTCTG 1702815 0.94979715 5.479185 210-219
CGTGG 2345560 0.9401331 7.4032054 50-59
TAGGC 1709175 0.93800855 5.8306003 160-169
TGAAC 1243445 0.9343816 6.494795 190-199
ACGTC 1504795 0.9234092 11.088195 70-79
CCATT 1272700 0.9213044 5.787428 50-59
ACTAA 893970 0.9198099 11.799296 240-247
ACCCA 1147860 0.9141542 11.925036 240-247
TGTAA 1151080 0.91257155 6.443106 230-239
TGCAT 1401075 0.90707225 6.003177 210-219
GAGTT 1566550 0.90704364 5.0082846 160-169
CCCAA 1123650 0.8948734 9.107756 240-247
GTATG 1528375 0.8849402 5.2332816 220-229
TCGAA 1175215 0.88311046 16.369799 70-79
TGAAG 1305270 0.8772058 9.313185 35-39
CATCA 1043000 0.87635195 5.598659 230-239
GGTGG 2426090 0.8696674 21.40721 25-29
AGCGT 1567745 0.86039066 6.527225 160-169
CCAGC 1474115 0.857402 5.737125 80-89
GAGGC 1832615 0.85257226 13.658338 30-34
CGGTC 1878625 0.8419367 5.4439783 130-139
AAAGA 785065 0.83849674 5.19867 8
CCAAT 994625 0.83570623 9.975364 240-247
ATCAA 810730 0.8341638 12.372105 70-79
CTTCC 1391360 0.82249975 5.368026 230-239
GGGCC 2148735 0.81632286 16.293997 15-19
GACCG 1559855 0.8114108 16.933268 45-49
TAACC 948160 0.7966652 6.047405 80-89
AACCG 1115605 0.7945924 5.743823 190-199
CGTCC 1576785 0.7901479 9.2609415 70-79
TGAGA 1152745 0.7747015 5.5104866 240-247
TGGGC 1902560 0.7625726 17.645977 15-19
GGTCC 1695375 0.7598102 12.310719 50-59
AAAGG 945195 0.73729235 8.411093 10-14
AGCCA 1029675 0.73338854 5.805772 80-89
CTAAT 823715 0.7301885 9.198772 240-247
GTCGA 1316080 0.7222749 11.814287 70-79
ATGAT 906725 0.71884793 6.669082 240-247
CATAC 847275 0.7118994 8.602704 70-79
GAAAA 663570 0.7087328 5.7214413 8
GGAAG 1241455 0.7072483 6.27737 30-34
GTAGC 1274780 0.69960916 5.3842864 80-89
AATCC 830200 0.6975526 8.761923 240-247
AAGGT 1007995 0.6774224 9.4293995 10-14
AAAGC 766470 0.6685133 9.303057 70-79
CATTG 1021565 0.6613732 5.2868385 50-59
AGGAT 949020 0.63778824 5.567194 10-14
TAGGT 1099555 0.6366503 5.353123 10-14
TATGA 794765 0.6300864 5.508592 240-247
AGAAG 803615 0.62685394 5.068901 9
GAGGT 1271355 0.62400854 6.674687 10-14
ATAGG 928120 0.6237424 5.102514 10-14
AAGAA 583785 0.6235176 5.150302 9
CAATC 741455 0.6229871 8.517809 240-247
TAAAA 480105 0.60491204 5.6203537 8
CGATG 1095715 0.60133696 13.224418 15-19
GGGTC 1487260 0.5961146 5.7519674 70-79
AATGG 869035 0.58403444 6.4786243 10-14
CATCC 818645 0.5617059 7.0870385 240-247
GAAGG 937675 0.5341869 5.4016895 10-14
AAGCA 593880 0.5179807 6.11164 70-79
AAGGG 907645 0.517079 6.182889 10-14
GGTCG 1245010 0.49901736 5.8334336 70-79
TGATC 760210 0.49216878 5.1896358 240-247
ATAAG 506275 0.46587035 5.194239 9
GATCC 758420 0.46540022 5.127464 240-247
ACGAT 610330 0.45862997 5.8582892 10-14
CGGTT 955590 0.45182958 8.067797 10-14
TAAGG 640485 0.4304375 5.610026 10-14
TAAAG 443185 0.40781543 5.5659056 9
AAGCT 540375 0.40606257 7.9769645 30-34
Produced by FastQC (version 0.10.1)