FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_9_SL248355.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_9_SL248355.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12195956
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 51678 0.4237306202154222 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 45017 0.369114155544674 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 42489 0.3483859731865218 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 42328 0.34706586347146545 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 39081 0.3204422843112914 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38342 0.3143828987247904 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37304 0.3058718808103276 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 36062 0.2956881772941785 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33716 0.2764522928747857 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32955 0.27021251962535775 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32425 0.2658668168366629 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32413 0.26576842356597546 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32087 0.26309540637896695 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31130 0.2552485430416443 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31009 0.254256410895546 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30518 0.25023048623658534 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30060 0.24647514307201504 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29311 0.2403337630932745 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29277 0.24005498215966012 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29200 0.23942362533941577 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29072 0.23837409711874985 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28830 0.23638983282655335 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28352 0.232470500877504 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27729 0.2273622502409815 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27400 0.22466463473630113 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27020 0.22154884783119913 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26582 0.21795749345110788 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25256 0.20708503704014677 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25174 0.20641268302378263 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24776 0.2031493062126495 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24659 0.20218997182344703 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24162 0.19811485052914263 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23393 0.19180948176592305 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22966 0.1883083212172953 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22945 0.1881361329935923 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 22685 0.18600427879536463 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 22671 0.1858894866462293 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 21739 0.17824760928950548 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21700 0.17792783115977132 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21587 0.1770012945274647 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21556 0.17674711191152215 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21475 0.17608295733438198 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21365 0.1751810190197472 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 20487 0.16798191138111684 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20485 0.16796551250266892 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20110 0.1648907227936867 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20035 0.16427576485189024 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19839 0.16266867476399555 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19427 0.15929050580372706 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19348 0.1586427501050348 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 19296 0.15821637926538928 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18545 0.15205860040820088 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18427 0.15109106657977447 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18409 0.15094347667374333 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17693 0.14507267818939326 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17328 0.14207988287265055 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17104 0.1402432084864852 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17100 0.1402104107295894 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16872 0.13834093858652818 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16772 0.13752099466413295 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15854 0.12999390945654443 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14973 0.12277020350024222 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14496 0.11885907099041682 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14433 0.1183425063193078 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14012 0.11489054240602377 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 12606 0.10336213085714642 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12414 0.1017878385261475 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12320 0.10101709123909598 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 24650000 7.636139 66.58422 35-39
TATTA 12259290 7.3024154 127.18393 240-247
TGGAT 18713880 7.1761236 79.359314 35-39
TCACC 19056730 6.421338 74.826256 160-169
TCTCC 16997425 6.145309 87.32665 20-24
CTGTG 17879655 5.9429135 87.25176 25-29
TCCCT 16024440 5.793532 72.5284 15-19
AGTGG 19198065 5.7023544 39.72761 100-109
CTGAG 18236165 5.6492443 50.37116 15-19
TGTAT 11222655 5.5558553 59.950672 240-247
TTACT 10288905 5.3123255 112.58142 240-247
ATTAC 10407345 5.008089 105.35157 240-247
TGGGT 15468540 4.9298015 45.458828 100-109
CCTGA 15101460 4.879058 52.014126 15-19
CTCTG 13659040 4.735007 73.581184 35-39
TTCAC 11218360 4.679244 74.719475 40-44
CTCCA 13666320 4.6049905 41.333473 80-89
GACAC 15275085 4.5995703 46.49331 230-239
ACACG 15236950 4.588087 49.045547 230-239
TGAGA 12486440 4.462531 94.03152 15-19
ATTCA 9024695 4.3427477 111.79595 40-44
TCTGG 12970945 4.3113365 134.83122 35-39
TACTG 10748535 4.298683 79.35531 240-247
AAGGG 15481040 4.285618 39.137856 90-99
AGCCT 12968630 4.189972 51.24937 30-34
CCCTG 14920855 4.1785436 85.077896 10-14
CAGCC 15923395 4.156077 55.577045 30-34
TGGAG 13950810 4.143775 38.687397 90-99
GAAGG 14928750 4.1327276 30.901693 90-99
CCAGG 16440390 4.114336 53.0484 80-89
ACCAT 10480695 4.0743 67.39652 160-169
GAGAC 13941360 4.0251184 85.515976 8
TCAGT 10035430 4.0134897 97.75167 7
TCCAG 12356915 3.992336 40.699524 80-89
GTATT 8003970 3.9624224 69.27249 240-247
CACCA 12595900 3.9556966 57.368553 160-169
GGGTC 15286430 3.9356549 125.39777 8
ACTAC 10076490 3.9171681 35.17562 130-139
TACTA 7987890 3.8438299 28.506609 130-139
CTGGG 14777175 3.8045418 133.15388 3
CACCT 11224960 3.7823522 44.03836 40-44
GACTC 11641950 3.7613413 52.281086 20-24
ACTGT 9398265 3.758667 68.404076 240-247
GATTC 9312870 3.724515 59.11847 40-44
TGAAG 10417940 3.7232697 39.464226 150-159
AGACT 9932170 3.7020898 69.01676 15-19
GGACA 12638290 3.6488984 28.437393 220-229
CAAGA 10309050 3.581278 43.666008 180-189
GCTGG 13798240 3.5525045 33.16261 2
ACTGG 11388470 3.5279484 44.24449 60-69
CCTGT 10125555 3.5100977 62.33368 25-29
ACTCT 8332840 3.4756768 68.76527 20-24
GAGTG 11479115 3.4096136 39.066936 90-99
TCCTG 9749840 3.3798537 64.537735 20-24
TATCT 6422810 3.316199 48.724472 200-209
CTCCT 9152100 3.308882 94.68105 20-24
GGATT 8474810 3.249795 56.518143 40-44
AGACA 9345940 3.246702 37.668797 170-179
GGTCC 12073840 3.2420259 141.66698 9
AGCTG 10431900 3.2316198 36.788162 60-69
CTGCA 9970110 3.2211947 44.74499 25-29
CATCT 7626875 3.1812146 33.13543 170-179
GTCTC 9050035 3.1372612 32.972202 30-34
CCATC 9283090 3.1280212 34.545357 45-49
GTCCC 10904895 3.0538855 75.25689 10-14
CCTCA 8928445 3.0085206 82.9283 5
GGAGT 10011280 2.973626 37.06905 90-99
GGCTG 11401200 2.935361 34.257843 90-99
AAGAA 7259270 2.9093645 36.892437 180-189
AGGGA 10421255 2.8849173 40.118126 80-89
AGTAG 7937055 2.8366256 28.37238 50-59
TGTGC 8502700 2.8261626 57.49437 25-29
GAGCT 9121415 2.8256547 29.53752 60-69
ATCTC 6750140 2.8155231 48.219025 170-179
GTGTA 7322910 2.8080816 36.496235 230-239
GTGAA 7795535 2.7860482 39.26396 10-14
ATCTG 6948520 2.7789357 36.61688 200-209
GGAAG 9940835 2.7519224 30.019163 80-89
GACAA 7917300 2.750404 24.877468 180-189
CAGGG 11424125 2.7412617 36.204453 80-89
ACCTT 6468715 2.698139 71.985275 40-44
CTATG 6726310 2.6900666 27.827803 50-59
GAACA 7700305 2.6750221 34.741524 210-219
ATGAA 6071285 2.6107733 27.519808 210-219
GGGAA 9429210 2.610289 33.656128 80-89
CACGG 10308135 2.5796914 44.280643 230-239
GAGTC 8253025 2.5566428 27.743965 150-159
TGCAA 6842665 2.5505161 35.603825 200-209
CGCCA 9753065 2.545593 39.85069 70-79
AGGGC 10557700 2.53336 32.964375 150-159
AGAAC 7265365 2.5239275 30.37257 190-199
CCCTC 8598770 2.5114684 27.064156 20-24
CAGTG 8100985 2.5095432 75.69559 8
ATCAG 6648740 2.4782329 26.478403 45-49
AGGCT 7965080 2.4674425 20.640247 25-29
CTACA 6343965 2.4661741 20.08096 190-199
CCAAG 8172160 2.4607668 38.403893 180-189
ACGGC 9802205 2.4530787 48.855488 230-239
TATAT 4099600 2.4419837 24.211044 240-247
GTGGA 8148055 2.420197 26.428621 100-109
TCTGT 5598920 2.4025576 24.381926 210-219
CACTG 7405310 2.3925457 19.513433 60-69
CTCTC 6550130 2.3681571 98.22991 20-24
GCAGC 9453875 2.3659065 34.307987 30-34
CCGCC 10373515 2.3468642 34.1123 70-79
CTGTC 6765920 2.345456 48.16726 30-34
ACAGC 7784065 2.3439054 26.847597 210-219
TGAAC 6281995 2.3415337 37.69526 210-219
CTACT 5589950 2.3316011 22.869713 50-59
TATGC 5821835 2.3283381 21.27752 50-59
CTTCA 5561040 2.3195426 50.613945 45-49
GGGCT 8965065 2.3081517 42.472862 90-99
GTATC 5761300 2.3041284 22.8555 200-209
CAAAT 5121385 2.2968686 35.183723 200-209
GTGGT 7100845 2.263029 31.04384 120-129
GGCCG 10873460 2.2615674 25.172712 230-239
CAGGC 9031360 2.2601688 33.560127 70-79
GCCTC 8067900 2.2593927 73.15566 4
TCCAA 5807615 2.2576716 42.183308 180-189
TGGTG 7038195 2.2430627 33.73964 35-39
GCCTG 8232905 2.2106712 31.242014 210-219
ACAAC 6080145 2.2028935 20.186035 180-189
AAATG 5112480 2.198468 33.387714 200-209
GCTCC 7846890 2.1974995 34.059155 80-89
AATGA 5104510 2.195041 25.998089 210-219
TACAT 4508125 2.169342 18.879446 190-199
TATCA 4506755 2.1686828 16.865334 170-179
GGGGG 11291805 2.15916 94.83512 5
CCTTC 5959895 2.1547613 55.484337 45-49
CAGTA 5779690 2.1543057 21.280575 45-49
GTGGG 8524530 2.1043658 30.03976 100-109
GCTAT 5259115 2.1032884 33.968605 50-59
CCTCT 5802415 2.0978253 49.165737 35-39
TGCAG 6748800 2.0906599 43.514496 30-34
GTAGT 5425495 2.0804887 24.772459 120-129
GCTGT 6238995 2.0737429 19.536495 230-239
GCAGA 7172300 2.07077 24.151909 140-149
TCTAT 3963795 2.0465703 19.701292 110-119
AACAG 5836055 2.027397 33.39738 210-219
TGCAC 6257790 2.021799 26.087175 25-29
CTCAC 5980255 2.0151012 54.53155 20-24
AACTA 4486745 2.0122416 36.742184 130-139
CAGAG 6951895 2.0071354 32.509396 170-179
ATGGA 5581900 1.9949163 12.427718 100-109
AGAGT 5562465 1.9879705 30.381195 150-159
TGTGA 5163825 1.9801475 18.678043 210-219
AGCAC 6560860 1.9755789 20.346205 130-139
GGCTC 7296730 1.9592928 19.77083 40-44
TGGTA 5108250 1.9588364 31.084644 120-129
TGAGC 6322890 1.9587206 17.876558 200-209
TCCGC 6960390 1.9492378 42.04599 70-79
TTCAG 4871095 1.9481066 47.30172 45-49
GGTCT 5852660 1.9453311 36.453175 15-19
TCTCT 4335770 1.9404209 32.706184 35-39
ATATC 4005755 1.927598 13.458169 160-169
CCGTG 7176000 1.9268748 20.2594 230-239
CTGTA 4805870 1.9220213 24.262749 190-199
CAGAC 6341400 1.9094961 26.334055 140-149
CATAT 3966220 1.9085735 14.221509 160-169
GCACA 6317250 1.9022243 18.571764 130-139
ACTAT 3924200 1.8883532 17.402023 130-139
GGTGG 7637415 1.8853724 18.072454 40-44
CCAGA 6252745 1.8828006 34.08616 170-179
TGGGG 7623225 1.8818696 123.75627 4
GCCAG 7515760 1.8808781 35.49318 70-79
GGATC 6061885 1.8778658 17.8982 70-79
GATGG 6299170 1.8710271 21.350235 100-109
AGGAC 6456915 1.8642261 20.879808 220-229
TCCAT 4454755 1.8581046 26.431433 45-49
GTCAC 5749150 1.8574651 38.072556 160-169
CCTGC 6621360 1.8542933 49.94795 20-24
AGAGA 5561520 1.8524768 28.1538 170-179
ACCAG 6148415 1.8513852 13.855469 190-199
ACCCT 5489780 1.8498312 43.078926 10-14
CTATT 3565285 1.8408134 19.29623 110-119
CACAG 6098250 1.8362797 16.212675 140-149
CAGTT 4574370 1.829437 24.451231 190-199
TTCCA 4375615 1.8250947 22.649395 180-189
TGGGA 6127030 1.8198968 16.958347 100-109
GCTGA 5856450 1.8142258 27.019728 200-209
TCAGC 5610635 1.812713 11.54538 45-49
GAGGA 6535480 1.8092177 27.213644 220-229
GCCGA 7175805 1.7958016 19.329208 220-229
TGTCT 4181860 1.7944818 49.073425 30-34
CAAGG 6211640 1.7934105 17.495016 80-89
ATACT 3698065 1.7795355 26.546455 130-139
TTCTG 4085115 1.7529674 43.578487 30-34
TAGTG 4560915 1.7489524 25.95129 120-129
TAGTA 3786605 1.7471167 16.851349 120-129
AGTCA 4676675 1.743171 28.696642 160-169
GCAAA 4996335 1.7356851 32.154995 200-209
ACCTG 5367140 1.7340431 30.392553 25-29
AGTTC 4319895 1.7276644 21.709131 190-199
CCTGG 6431100 1.7268567 34.15782 2
AAAAA 3574295 1.7236198 10.090995 90-99
TACAA 3831355 1.7183084 17.510466 130-139
CATGA 4599075 1.7142466 23.10969 60-69
CTCAG 5282695 1.7067604 79.21632 6
ATGCA 4563680 1.7010535 16.629915 60-69
CTGAA 4560210 1.6997602 22.87706 200-209
TGACC 5260550 1.6996057 14.267133 210-219
GTGCA 5456020 1.6901796 50.320667 25-29
GGATG 5686965 1.6891851 22.77135 100-109
AGCTA 4503025 1.6784452 25.704334 50-59
GGAGC 6953395 1.6684933 13.35843 200-209
AACAC 4577720 1.6585509 22.309444 190-199
ATTGG 4259975 1.6335523 20.28748 100-109
GCCGT 6062145 1.6277864 29.323305 230-239
TCTGC 4641050 1.6088542 33.48613 200-209
AGGGG 6973690 1.6044643 32.7525 90-99
TCTCA 3842680 1.6028045 26.22423 100-109
CAGCT 4959865 1.6024588 12.9336605 50-59
CGATT 4003515 1.6011338 35.372475 160-169
TCTGA 3996420 1.5982963 21.689178 220-229
GGGCC 7663855 1.5940026 28.69824 2
GGGGC 7927945 1.5810378 28.607141 90-99
CTCCC 5328520 1.5563167 14.025493 190-199
ACAAT 3441840 1.5436164 22.862722 180-189
GGCTT 4625985 1.5376039 21.889338 25-29
GCACT 4755275 1.5363588 25.013386 25-29
GTTCT 3574980 1.534063 22.984184 190-199
AGTGA 4264320 1.5240264 86.88438 9
CCATG 4702460 1.519295 15.648302 160-169
CGTCC 5420735 1.5180616 18.781952 180-189
AGTTA 3270020 1.5087677 15.859228 50-59
CGGCC 6934015 1.5041348 31.589466 230-239
GAGCA 5208185 1.5036956 7.846536 200-209
TTCTC 3354780 1.5013908 21.81972 190-199
GTCCG 5587660 1.5003792 34.513374 70-79
TTAGT 2991430 1.4809288 25.365185 110-119
CATCA 3784165 1.4710689 30.805252 45-49
CTTTA 2848650 1.4708033 42.07044 45-49
GTTAC 3620410 1.4479178 15.643321 50-59
ACTCC 4294880 1.4471989 16.960098 140-149
CTGGT 4349990 1.4458677 31.12829 35-39
TACGC 4432800 1.432172 24.42493 140-149
GTGAC 4545780 1.4082031 14.953667 210-219
GGGGT 5664515 1.3983423 117.759476 7
CGTGT 4200510 1.3961829 24.115852 230-239
GGCAG 5803270 1.3925164 13.803911 150-159
CTCAA 3556810 1.3826861 18.362743 150-159
GGCCT 5145540 1.3816626 69.862114 3
GTCCA 4276290 1.3816059 28.519667 180-189
TAGCA 3687050 1.3743008 11.569056 130-139
CAACT 3521925 1.3691248 17.439978 130-139
CAACC 4346135 1.364888 15.090651 140-149
CGACA 4510605 1.3582147 16.497475 70-79
CTACG 4203585 1.358116 16.250519 130-139
AAGAG 4067160 1.354723 18.520275 150-159
AACCC 4312655 1.3543737 19.923931 140-149
GAGAG 4884500 1.3521767 25.690792 220-229
GAACT 3625735 1.3514465 14.71878 190-199
AGCAG 4672110 1.348921 12.272705 50-59
AAGCT 3611635 1.3461907 16.348427 200-209
TGGAA 3728545 1.3325456 19.395245 120-129
ATACA 2960350 1.327675 22.096746 35-39
GTGCG 5132995 1.3215445 17.273808 240-247
ACAGA 3795825 1.318638 20.442974 140-149
ACAGT 3537650 1.3186138 12.301657 120-129
ATCTA 2739305 1.3181732 20.292906 110-119
ACATG 3529485 1.3155704 14.842256 190-199
TGTGT 3175305 1.3064562 15.273072 240-247
CCTTT 2917205 1.3055594 48.351498 45-49
TTACA 2711305 1.3046994 9.682102 120-129
TTATT 2039275 1.3033481 20.455124 240-247
CCATA 3350090 1.302325 14.679943 160-169
CACGT 4027450 1.3012092 16.425297 180-189
GTGGC 5044520 1.2987657 14.070028 40-44
TCACT 3112700 1.2983255 13.531557 190-199
CAATT 2692590 1.2956936 23.81055 180-189
TCAAG 3457320 1.2886719 22.785847 150-159
AGAGC 4450805 1.2850266 24.55639 220-229
TGTCC 3700785 1.2829044 53.48921 15-19
TGCGA 4131825 1.2799671 14.2769375 240-247
TCCGT 3673045 1.2732879 13.709369 150-159
CCTAC 3764255 1.2684 17.740612 190-199
AATTC 2631450 1.2662725 23.80865 180-189
ACATA 2815930 1.2629048 20.825808 130-139
AACCA 3484985 1.2626425 17.2592 190-199
ATATT 2118145 1.2617025 25.862326 240-247
ATTAT 2113620 1.2590071 9.879688 50-59
CCAGT 3886530 1.2556801 18.644829 190-199
ACACA 3451865 1.2506429 7.90215 130-139
ATCAT 2581580 1.2422748 15.802433 110-119
AGACC 4104610 1.2359632 98.37426 9
CGGCT 4600330 1.2352648 21.406002 230-239
CTTCT 2757290 1.2339914 23.759726 30-34
ACGTC 3766080 1.2167646 18.205322 180-189
GATTG 3166155 1.2141105 23.387423 100-109
TGGAC 3911580 1.2117391 16.106367 80-89
ATCCG 3743815 1.2095711 23.41373 70-79
TGAGT 3144365 1.2057546 21.254875 90-99
GATCT 3006540 1.2024117 9.568722 220-229
AGTCG 3880830 1.2022133 15.806543 150-159
TACCA 3091210 1.2016872 11.00851 45-49
TCGGA 3834040 1.1877185 91.970085 5
AAGGC 4068860 1.174752 28.98038 25-29
GGGTG 4737810 1.1695758 14.812984 60-69
GTCGA 3757920 1.1641378 12.307074 150-159
GAAGT 3246180 1.1601529 13.713863 140-149
ATTAG 2505120 1.1558473 25.025959 110-119
ACGCA 3835250 1.1548547 20.837135 140-149
AGATC 3096735 1.1542685 12.87503 210-219
AGGCC 4608440 1.1532984 13.83657 2
CTTCG 3318975 1.1505471 103.7775 3
GATCC 3556685 1.1491122 23.273184 70-79
CGCTG 4262705 1.1446068 12.250422 190-199
ATTCC 2738820 1.142378 22.120546 180-189
GGACT 3635710 1.1262792 12.854767 90-99
AGCTC 3476925 1.1233429 12.151723 210-219
GCAAG 3890490 1.1232535 17.105015 25-29
ACAAG 3227180 1.1210954 17.658846 80-89
AGGTC 3610475 1.118462 18.188345 10-14
TCGAG 3605460 1.1169083 12.011825 150-159
TTGGG 3479515 1.1089163 15.088904 100-109
GCCCC 4882565 1.104613 12.276814 80-89
GAACC 3665315 1.1036845 10.357107 180-189
GACCC 4224405 1.1025884 42.33414 10-14
GTATA 2386660 1.1011906 10.211698 240-247
GCTTC 3166510 1.097694 27.73032 2
CTTGA 2744125 1.0974635 22.582462 90-99
CCGAT 3392775 1.0961552 23.644407 160-169
TTGAG 2856805 1.0954853 20.964794 90-99
CACAT 2802345 1.0893929 13.822424 130-139
CCCCT 3727880 1.088813 9.867862 70-79
TTCGG 3271570 1.0874178 99.45505 4
CGCAG 4326680 1.0827857 16.402029 140-149
ATGGG 3633635 1.0792897 20.277039 100-109
GCACC 4120140 1.0753748 10.971978 130-139
ATCAA 2381395 1.0680219 12.6122055 110-119
TACAG 2862245 1.0668652 9.162855 120-129
GAAGC 3676550 1.0614853 12.771953 200-209
ACACC 3378900 1.0611312 13.246957 40-44
TAGAC 2833385 1.0561081 17.083708 170-179
CAGCA 3489795 1.0508327 12.58798 45-49
ATGGT 2730270 1.0469637 9.516808 35-39
CTCCG 3722165 1.0423818 9.829112 140-149
ATGAG 2907335 1.0390531 19.926748 60-69
CTATA 2150790 1.0349755 13.302675 50-59
GGAGA 3728335 1.0321153 81.785446 7
TGGCT 3104360 1.03184 30.85747 40-44
GACAG 3569215 1.0304956 15.867097 70-79
GAGCC 4113500 1.0294358 17.982285 220-229
GGGAG 4415305 1.0158465 14.445788 5
AAACT 2260840 1.0139548 17.292423 130-139
TGAGG 3411115 1.013195 12.115446 220-229
CGTGA 3269985 1.0129842 20.313976 150-159
GCTCT 2917685 1.0114368 16.973263 210-219
ACCGC 3870225 1.0101458 10.981285 220-229
GTAGC 3251960 1.0074003 11.2428055 50-59
GTAGA 2818595 1.0073383 16.719364 170-179
CGAGG 4187895 1.0049012 14.645799 220-229
TAGTT 2024130 1.0020599 14.691968 50-59
AGCCG 3993195 0.99932843 19.000532 220-229
TTTCA 1932090 0.9975686 7.3009624 100-109
ACTCA 2561155 0.99563193 12.409743 190-199
AACTC 2547455 0.9903061 16.544115 190-199
ATGAC 2648870 0.9873325 11.034635 160-169
AAGGT 2754685 0.9844973 20.868713 15-19
GCCAA 3256485 0.9805793 14.91394 180-189
TTATA 1643290 0.97884834 13.152805 110-119
ACCAA 2663145 0.96488225 16.807936 130-139
ATGAT 2087760 0.9632799 8.928854 120-129
CCGTC 3435895 0.9622128 10.3561 140-149
GATAC 2576110 0.9602122 24.02223 35-39
GGTAT 2502685 0.9596927 9.364807 110-119
TTTAG 1931035 0.9559726 29.548609 45-49
CCGAG 3811915 0.95396155 17.847326 220-229
CGAGT 3071630 0.95153713 14.786911 160-169
CCAAC 3020900 0.9487027 13.934733 130-139
GGGAC 3933235 0.9437945 9.892274 90-99
CAGAA 2716140 0.94356436 18.353828 140-149
ACAGG 3267435 0.9433664 15.418669 70-79
GCAGT 3042380 0.94247603 6.063002 50-59
GCCAT 2916330 0.94222295 7.74064 60-69
CGGAC 3757720 0.940399 10.817468 220-229
AACTG 2504990 0.933703 11.859717 60-69
CATAC 2399860 0.9329295 14.077381 130-139
TTTAT 1451870 0.92792386 10.743663 240-247
TACAC 2379595 0.9250516 22.31415 35-39
TATGG 2408280 0.92349166 12.329718 35-39
TAACA 2051615 0.92012024 8.76823 120-129
AGCCC 3523920 0.91975874 11.281769 80-89
AATCA 2045685 0.91746074 13.903516 110-119
GAGAT 2561275 0.9153746 12.379286 210-219
AAGTT 1974195 0.9108817 17.728937 140-149
TGGTT 2198525 0.9045672 9.193185 50-59
CGTAT 2250065 0.899873 7.5038924 240-247
CCCAG 3438605 0.8974911 14.620365 80-89
TCATA 1858260 0.894208 8.655898 110-119
TCCTT 1996795 0.8936412 10.8231325 45-49
CCCCA 3276430 0.89188504 16.75932 80-89
TATTT 1385310 0.88538384 10.997419 240-247
GTTAT 1783710 0.88303834 10.746325 110-119
AGAAG 2637280 0.8784469 16.043783 140-149
GACTG 2823000 0.87451595 12.508514 90-99
AGTCT 2171625 0.86850244 15.811168 8
GATGA 2422580 0.86580646 6.7525005 60-69
GCCTA 2667225 0.8617408 17.061182 190-199
CGGAG 3589035 0.8612025 71.12567 6
TTACC 2062835 0.86042064 10.195788 45-49
GATCA 2299270 0.8570236 9.471183 110-119
ATCCA 2197975 0.85444814 9.987873 180-189
GTTTC 1987000 0.8526434 5.0604916 100-109
CATGG 2751395 0.85233396 8.791074 200-209
ACGCT 2636370 0.85177207 15.020619 190-199
GTCTG 2546145 0.8462982 14.510622 3
ATAGT 1831560 0.8450708 15.404803 120-129
GGTAG 2821720 0.8381285 11.901407 120-129
CTATC 2002085 0.83508146 8.373954 110-119
CATTA 1725580 0.8303615 12.219598 110-119
GCTTG 2496345 0.8297455 17.290716 90-99
TCTTC 1851805 0.8287528 44.589115 2
TCATC 1985270 0.82806784 10.705667 110-119
GGTTC 2487550 0.82682204 11.2233 160-169
GTCTA 2067140 0.82671547 9.959149 30-34
ACAAA 1966120 0.82181746 12.98738 130-139
GGTGC 3185500 0.820141 11.880659 60-69
TGGCA 2642290 0.8185352 5.7147226 100-109
TTATG 1635790 0.8098095 11.586181 50-59
ACATC 2073365 0.8060068 5.6088634 110-119
ATATG 1745940 0.8055662 5.724056 50-59
ATATA 1443805 0.8015422 10.572176 130-139
CCTCC 2719485 0.7942881 10.4347725 140-149
TGATG 2049685 0.78598297 11.98608 120-129
TATAC 1581645 0.7610989 8.863476 130-139
GTTCA 1887110 0.7547157 13.619641 160-169
CCAGC 2887590 0.75367373 6.2386365 80-89
GGGAT 2536420 0.75338656 13.611332 100-109
CTGAC 2328950 0.7524492 9.656734 220-229
TCAAC 1919665 0.74625695 7.640887 110-119
CATCC 2206985 0.74366367 6.774637 110-119
GGTTA 1928555 0.7395338 8.115568 50-59
CCCCC 3105100 0.7326521 13.311205 80-89
TTTAC 1416845 0.7315395 13.7713375 45-49
ATCTT 1415640 0.73091745 11.945936 3
TTGGA 1902150 0.7294084 6.427253 60-69
ATAAA 1403350 0.72610676 7.704643 130-139
GCGGA 3022710 0.7253107 9.016913 220-229
GATTA 1566890 0.72295374 5.7676554 50-59
AACGC 2398295 0.72216463 14.55398 180-189
ACGCC 2760235 0.7204336 13.109294 180-189
TATTG 1451380 0.718516 6.500145 240-247
CGCCG 3302675 0.7164201 10.7978325 220-229
GGGCA 2970635 0.71281505 13.796829 150-159
GACCG 2847715 0.71266305 9.214863 210-219
ATGCC 2197705 0.7100459 8.043968 50-59
CAACG 2343205 0.7055762 14.841991 180-189
GACCA 2336465 0.7035467 6.238489 170-179
TGCCA 2174320 0.70249057 7.439003 60-69
TCCGG 2611010 0.7010994 11.508206 70-79
GGATA 1960140 0.7005348 15.806286 35-39
TTTCT 1264495 0.70051265 9.302079 4
CACAA 1932750 0.70025337 8.430544 130-139
AATAC 1551380 0.69577193 11.283858 130-139
CAAAC 1913775 0.6933786 13.971702 130-139
AAATA 1326585 0.6863878 12.536784 130-139
GAAAT 1591160 0.68423045 7.257648 110-119
TATAG 1472045 0.6791927 6.2180486 120-129
CAGTC 2077090 0.67107695 5.478359 210-219
AGGCA 2296990 0.66318184 6.5456147 80-89
GCGAC 2623405 0.65652776 7.321027 70-79
GCCGC 3016330 0.6543059 11.852508 220-229
CCCGT 2332125 0.6531051 10.126341 140-149
GACGA 2251955 0.6501793 9.898785 220-229
CACGC 2479875 0.64725834 12.181428 190-199
TCCAC 1916640 0.64582926 10.572356 180-189
AGATG 1806155 0.6455021 5.8556447 120-129
AATAA 1241460 0.64234334 7.1685686 130-139
ACTTC 1511705 0.6305411 29.951082 4
TACTT 1220995 0.63041914 10.994192 1
ATTAA 1128780 0.6266531 6.4544134 110-119
CACCG 2399245 0.62621355 9.143098 45-49
TCAAT 1299555 0.6253552 8.449519 110-119
AAGCA 1765745 0.6134051 5.9011035 120-129
CACGA 2032910 0.61214143 8.983642 180-189
CCCTA 1814035 0.6112556 11.981258 110-119
CCGGC 2810405 0.6096363 9.342078 70-79
GGCCC 2795600 0.6064248 10.523922 70-79
CCGTA 1873525 0.6053082 6.270333 230-239
AATGG 1692880 0.60501873 6.8378816 120-129
TAGCT 1500490 0.60009396 12.368944 50-59
AGTAA 1385295 0.59570444 5.2174397 50-59
CTTTG 1381285 0.59272444 7.375396 45-49
ACGAC 1968000 0.592596 9.256598 220-229
TGATT 1190650 0.58943975 6.2314496 50-59
GGTGA 1969360 0.58495414 6.561124 120-129
GGGGA 2522695 0.5804063 9.220779 4
GTTCC 1671665 0.57949495 6.9604173 140-149
GAAGA 1737820 0.5788474 6.4977646 15-19
ATTTC 1103705 0.56986034 6.5080967 240-247
ACCCG 2176045 0.56795734 8.621606 140-149
ACCTA 1440845 0.56011885 6.9214854 130-139
TTCAA 1161870 0.55910015 5.4758196 200-209
TCAGG 1803220 0.55860597 5.4115357 150-159
TAATA 1004215 0.5574996 7.861193 130-139
AAGAT 1287130 0.5534915 5.935309 15-19
TTAGC 1382585 0.55294 9.2871 45-49
GTCCT 1591605 0.55174154 6.4778996 45-49
CGGTT 1649815 0.5483723 11.111084 160-169
CCACG 2085000 0.5441942 7.9252315 180-189
CCTAT 1297955 0.5413847 5.204671 110-119
CGGCA 2163210 0.5413603 10.693737 70-79
CTGCG 2011160 0.5400297 11.914663 25-29
GGAGG 2346735 0.5399225 5.306066 6
CTGTT 1252570 0.53749144 5.8126574 240-247
GCTAC 1656470 0.53518087 8.03024 50-59
AAATC 1179970 0.5291998 7.847771 110-119
TATGT 1061265 0.5253868 6.762029 140-149
ACCCC 1878985 0.51148313 8.91711 140-149
ACATT 1057825 0.50903296 6.107155 110-119
GGGTT 1562075 0.49783102 5.849525 100-109
GTAAC 1326405 0.49440056 6.9970517 120-129
TGACG 1594740 0.49402252 8.777279 220-229
TGGCC 1813005 0.4868219 15.819545 2
TATCC 1159180 0.4835008 5.5747037 110-119
GGGTA 1619300 0.4809767 5.090893 100-109
CGCGG 2311145 0.48069432 6.430605 220-229
AACAT 1064625 0.47746924 5.8172092 110-119
CAATC 1190145 0.4626609 6.340707 110-119
GGTTT 1123630 0.46230936 5.419135 100-109
CATAA 1026190 0.46023166 5.9946012 110-119
ATGCT 1149470 0.45970985 7.1375103 50-59
CCGGT 1692055 0.45434475 8.658137 160-169
TAAAT 817155 0.45365146 7.7017317 130-139
TGCTA 1125780 0.45023546 6.336478 50-59
CCGCG 2053765 0.44550508 6.6573906 220-229
ATTCT 852470 0.44014382 7.5918794 2
TGCGC 1632690 0.43840432 8.868159 25-29
GCCTT 1256835 0.43569106 9.13414 1
TTGGT 1056190 0.43456167 5.314232 120-129
GGTCA 1383645 0.42862898 5.5666237 160-169
GCATG 1361860 0.42188036 8.37966 60-69
GCGCT 1544470 0.4147158 8.024561 25-29
ACGAG 1423995 0.4111326 7.855346 180-189
CACTT 985030 0.4108618 6.931582 3
ATGGC 1312690 0.40664837 7.289329 1
TACCT 968085 0.40379396 5.2088084 2
AGCTT 998080 0.39916414 7.55169 1
CAATG 1069980 0.39882135 5.069653 120-129
CTAAC 1021910 0.39726067 5.5145807 110-119
CATGC 1228230 0.3968229 7.083208 60-69
TTTGA 801190 0.39663482 6.2612305 45-49
CCTAA 1006410 0.39123517 7.6151557 110-119
CCGGG 1878230 0.3906524 5.2770615 80-89
GGTAA 1076115 0.38459295 7.3344293 120-129
GAGGC 1568530 0.3763747 5.922663 35-39
TTCTT 648360 0.35918242 18.647345 1
CGAGC 1378295 0.34492913 6.687187 180-189
ATGTG 895615 0.34343722 5.105153 140-149
TGGGC 1328965 0.34215626 9.861624 1
TGCTG 1018035 0.33837867 13.8346195 1
CTCGG 1219080 0.32734314 5.3993726 6
GCTTT 731205 0.31376803 5.3346257 45-49
CTCTT 699985 0.31326973 8.769567 1
GCGAG 1264160 0.30333996 5.438491 240-247
CGCTT 864415 0.2996558 5.4645557 1
AACTT 612845 0.2949054 10.095165 3
CGGGA 1220940 0.29296917 5.0023565 80-89
TCACA 753440 0.29289478 5.067687 5
GACTT 726700 0.29063058 6.564173 3
TTCCT 646985 0.28955022 6.3725295 4
ATTGT 572015 0.2831801 5.3279123 240-247
GAGGT 924925 0.2747282 6.682396 7
CCCTT 751620 0.2717433 5.3945904 3
TGTAA 571580 0.26372358 8.059166 25-29
TAACT 544065 0.261808 7.050679 2
TTGGC 787300 0.261686 7.9369335 1
GTCTT 599625 0.25730562 11.96344 1
AATCT 533635 0.256789 7.4661593 2
CGGGG 1270170 0.25330484 9.07077 3
TCGGT 740145 0.24601245 6.4554405 7
CCTCG 864540 0.242112 5.931505 5
TGCTT 509295 0.21854404 11.110613 1
TCGCA 546395 0.17653212 6.0361705 7
TAGGC 502670 0.15571836 6.549682 1
TTGCT 294035 0.12617362 5.83822 4
Produced by FastQC (version 0.10.1)