FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11380873
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 50436 0.44316459730286073 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 41619 0.3656925088259925 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 40113 0.3524597805458334 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38132 0.3350533829874035 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36427 0.32007210694645305 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36136 0.31751518534650197 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33309 0.292675263136668 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33217 0.2918668892975082 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 33131 0.2911112354913371 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 32144 0.2824387900646989 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31323 0.27522493221741423 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31286 0.2748998253473174 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30326 0.2664646200691283 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29712 0.26106960335995316 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29224 0.25678170734354033 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28967 0.2545235325971918 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28946 0.2543390124817314 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28908 0.25400511893946975 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27020 0.23741588189236448 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26897 0.2363351212160965 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26469 0.23257442552957053 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26439 0.23231082536462716 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26438 0.23230203869246233 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26268 0.2308083044244497 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26083 0.22918277007396531 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25045 0.22006220436692334 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24828 0.21815549650716604 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23747 0.20865710389703848 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23223 0.20405288768269358 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 23084 0.20283154025178912 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23028 0.20233948661056142 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22859 0.20085453901471356 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22394 0.19676873645809068 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22239 0.19540680227254975 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21723 0.19087287943552309 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21689 0.19057413258192057 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21568 0.18951094524998213 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20931 0.18391383508101708 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 20831 0.18303516786453902 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 20187 0.17737655099042052 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20128 0.17685813733269848 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20015 0.1758652433780783 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19229 0.16895891905656096 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18793 0.16512792999271675 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18586 0.1633090888546072 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18272 0.1605500737948662 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17971 0.1579052854732673 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17940 0.1576328986361591 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17515 0.15389856296612747 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17263 0.15168432158060283 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17162 0.15079686769196002 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17147 0.1506650676094883 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17070 0.14998849385280022 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16446 0.1445056104219773 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16308 0.1432930496632376 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15281 0.1342691373500082 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15048 0.1322218427356144 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14965 0.13149254894593762 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14899 0.13091262858306213 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14825 0.13026241484286838 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14276 0.12543853182440398 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13835 0.12156360939973584 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13741 0.1207376622162465 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13324 0.1170736199235331 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 12986 0.11410372473183736 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12677 0.11138864303292023 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12161 0.1068547201958936 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 23229275 7.7002263 67.9509 35-39
TATTA 11534795 7.3923883 129.54742 240-247
TGGAT 17604690 7.217441 80.5367 35-39
TCACC 17936405 6.4825015 75.53602 160-169
TCTCC 16082990 6.201084 88.649895 20-24
CTGTG 16851345 5.9593105 88.57224 25-29
TCCCT 15081640 5.814995 73.125175 15-19
CTGAG 17161560 5.688852 51.557835 15-19
AGTGG 17904095 5.6839523 40.28698 100-109
TGTAT 10559080 5.590334 60.894802 240-247
TTACT 9606815 5.31081 112.32541 240-247
ATTAC 9677995 5.015026 106.42456 240-247
CCTGA 14335415 4.9618974 53.334656 15-19
TGGGT 14625225 4.953295 46.149136 100-109
CTCTG 12958735 4.785129 74.71691 35-39
TTCAC 10704925 4.784956 75.97673 40-44
CTCCA 12981965 4.691888 42.421535 80-89
GACAC 14383510 4.6666884 48.035522 230-239
ACACG 14363830 4.6603036 50.449486 230-239
TGAGA 11838605 4.5494804 96.92985 15-19
ATTCA 8662445 4.4887795 114.837425 40-44
TCTGG 12272130 4.339916 136.7658 35-39
AAGGG 14570015 4.3357525 39.90778 90-99
TACTG 10021045 4.2898054 78.94872 240-247
AGCCT 12258725 4.243096 52.491077 30-34
CAGCC 15039495 4.2090464 56.947243 30-34
GAAGG 14060630 4.18417 31.624762 150-159
CCCTG 14010310 4.1830373 86.00308 10-14
CCAGG 15593205 4.179421 53.901306 80-89
ACCAT 9858490 4.130581 67.66566 160-169
TGGAG 12990060 4.1239104 38.788082 90-99
GAGAC 13271110 4.123647 85.83432 8
TCCAG 11789645 4.080734 41.825764 80-89
TCAGT 9333670 3.9955544 95.59712 7
GGGTC 14570670 3.990103 128.34483 8
GTATT 7516640 3.9795632 69.870636 240-247
CACCA 11704075 3.965069 57.89951 160-169
ACTAC 9394030 3.935978 34.672638 130-139
TACTA 7526560 3.9001772 28.9829 130-139
CTGGG 14102015 3.8617644 135.72116 3
GACTC 11152220 3.8601027 53.7266 20-24
GATTC 8968880 3.839395 60.54859 40-44
AGACT 9490715 3.8082893 71.267876 15-19
CACCT 10484565 3.7892883 44.202187 40-44
ACTGT 8817220 3.7744725 68.02035 240-247
TGAAG 9753785 3.7483013 40.451515 150-159
GGACA 11903625 3.6987371 28.811594 220-229
CAAGA 9671330 3.637673 44.452457 180-189
ACTGG 10767530 3.5693069 44.65651 60-69
ACTCT 7970470 3.5626915 70.681915 20-24
GCTGG 12992430 3.55791 33.597145 90-99
CCTGT 9540155 3.5227878 63.09471 25-29
TCCTG 9218410 3.4039805 64.974525 20-24
TATCT 6100610 3.3725207 49.987343 200-209
GAGTG 10611550 3.3688133 39.018555 100-109
CTCCT 8627315 3.326415 95.665085 20-24
AGACA 8793630 3.307544 37.79266 170-179
GGATT 8031355 3.2926362 57.881042 40-44
GGTCC 11475155 3.2812011 144.28212 9
CTGCA 9401805 3.2542343 45.42921 25-29
CATCT 7219400 3.2269733 33.90411 170-179
AGCTG 9561165 3.1694112 35.928658 60-69
GTCTC 8521125 3.1465018 33.57153 30-34
CCATC 8663865 3.1312582 34.97322 45-49
GTCCC 10360565 3.0933385 77.01741 10-14
GGAGT 9485620 3.011368 37.08042 90-99
CCTCA 8287060 2.9950747 81.28399 5
GGCTG 10908160 2.9871435 34.8644 90-99
AAGAA 6771435 2.952648 37.359085 180-189
AGTAG 7505460 2.884288 29.07929 50-59
AGGGA 9625075 2.8642352 40.62861 80-89
TGTGC 7987710 2.8247738 58.37451 25-29
GTGAA 7324720 2.814831 39.56684 150-159
ATCTG 6549940 2.803896 37.39713 200-209
GACAA 7446375 2.8008018 25.552303 180-189
ATCTC 6253040 2.795024 49.31944 170-179
GAGCT 8383120 2.7789037 29.115713 60-69
GAACA 7353225 2.7657652 35.987698 210-219
CAGGG 10716670 2.750875 36.58211 80-89
GGAAG 9221990 2.7442849 30.425957 80-89
ATGAA 5854510 2.7234232 28.515987 210-219
ACCTT 6052225 2.7052622 71.73153 40-44
GTGTA 6577855 2.6967406 35.779163 230-239
CTATG 6180545 2.6457655 27.091812 50-59
CGCCA 9340415 2.6140664 40.621864 70-79
CACGG 9731605 2.608346 45.18269 230-239
GGGAA 8733830 2.5990179 34.140682 80-89
TGCAA 6439675 2.5840147 36.355732 200-209
AGAAC 6796110 2.556218 31.07995 190-199
GAGTC 7700175 2.552516 27.067862 150-159
AGGGC 9856425 2.5300577 33.20593 150-159
AGGCT 7564185 2.5074368 20.584354 80-89
CAGTG 7516620 2.4916692 73.95877 8
CCAAG 7663410 2.4863713 38.863323 180-189
ACGGC 9271895 2.4851305 49.638405 230-239
CCCTC 7937320 2.4745007 26.851084 20-24
CTACA 5853920 2.4527175 19.767752 190-199
CACTG 7069075 2.4468093 19.773607 60-69
TATAT 3813485 2.443976 24.659641 240-247
ATCAG 6064400 2.4334302 26.881535 45-49
TCTGT 5311260 2.4255805 24.026278 210-219
TGAAC 5994485 2.4053755 38.76249 210-219
CTCTC 6217365 2.3972158 100.462074 20-24
GTGGA 7534200 2.391857 26.252216 100-109
CCGCC 9850000 2.3778977 34.782894 70-79
GCAGC 8864795 2.3760164 35.156982 30-34
ACAGC 7323055 2.3759441 27.519571 210-219
CAAAT 4855225 2.3583248 36.199596 200-209
CTACT 5262145 2.3521075 22.699917 50-59
GGGCT 8571340 2.347217 43.020676 90-99
CTTCA 5249755 2.346569 51.03719 45-49
CTGTC 6303955 2.3277922 47.696228 30-34
CAGGC 8553580 2.2926018 33.81258 70-79
TATGC 5351895 2.2910373 20.783728 50-59
GGCCG 10337115 2.2888477 25.007612 230-239
GTATC 5345835 2.288443 23.428783 200-209
AAATG 4888930 2.2742507 34.319023 200-209
GCCTC 7596560 2.2680936 71.138916 4
AATGA 4872445 2.2665823 26.882067 210-219
ACAAC 5770895 2.2664719 21.210255 180-189
TCCAA 5405490 2.264831 42.168884 180-189
GCCTG 7793655 2.2285144 31.213087 210-219
TGGTG 6565000 2.223445 33.588993 35-39
GCTCC 7436285 2.2202406 34.793835 80-89
GGGGG 10805560 2.1944482 96.84824 5
TACAT 4195730 2.1741793 18.52213 190-199
CAGTA 5412065 2.171671 21.567444 45-49
GTGGT 6382670 2.161693 30.642305 120-129
CCTTC 5539745 2.1359475 55.7508 45-49
TGCAG 6401065 2.121876 44.54886 30-34
GTGGG 8090285 2.1217718 30.625654 100-109
GTAGT 5165115 2.1175559 25.14264 120-129
CCTCT 5472970 2.1102011 50.029747 35-39
AACAG 5557720 2.0904226 34.467262 210-219
GCAGA 6726615 2.090118 24.658827 140-149
TATCA 4004365 2.075016 16.72866 170-179
GCTGT 5856430 2.0710678 19.64493 230-239
GCTAT 4820690 2.0636392 32.92971 50-59
TCTAT 3729600 2.0617864 19.49032 110-119
CAGAG 6589310 2.047454 33.46032 170-179
TGCAC 5850200 2.0249217 26.507654 25-29
CTCAC 5574210 2.014608 54.099262 20-24
ATGGA 5204470 2.0000362 12.319813 100-109
TGTGA 4862160 1.9933525 18.256712 210-219
GGCTC 6957650 1.9894676 19.764181 40-44
TCCGC 6624790 1.9779537 42.85264 70-79
AACTA 4070525 1.977173 35.560635 130-139
TTCAG 4585100 1.962788 47.89273 45-49
CTGTA 4578745 1.9600677 24.689226 190-199
TGAGC 5887995 1.9517998 17.354876 200-209
TCTCT 4086415 1.9486344 33.04514 35-39
AGCAC 5986180 1.942199 20.277573 130-139
AGAGT 5047165 1.9395849 29.88422 150-159
GGTCT 5480650 1.9381769 35.63734 15-19
CCGTG 6760100 1.9329802 19.776575 230-239
CATAT 3727240 1.931413 13.682396 160-169
CCAGA 5931885 1.9245831 35.174797 170-179
ATATC 3713125 1.9240988 13.409279 170-179
CAGAC 5927925 1.9232982 27.012667 140-149
GCCAG 7148645 1.9160395 35.960735 70-79
TGGGG 7275185 1.9080024 126.055244 4
AGAGA 5294540 1.9071988 29.086227 170-179
AGGAC 6116325 1.9004867 21.347076 220-229
TGGTA 4633980 1.899805 30.458786 120-129
ACTAT 3651300 1.8920618 17.042412 130-139
GATGG 5886220 1.8686783 21.014507 100-109
GCACA 5751305 1.8659945 18.283659 130-139
TCCAT 4170035 1.863949 26.771643 45-49
CTATT 3358745 1.8567712 18.693577 110-119
GGATC 5599435 1.8561457 18.124294 70-79
ACCAG 5693610 1.8472754 13.961335 190-199
TGGGA 5805170 1.8429477 16.669556 100-109
ACCCT 5097995 1.8424963 42.671898 10-14
GTCAC 5322255 1.8421848 37.671738 160-169
CAGTT 4298285 1.8400083 24.3509 190-199
GAGGA 6163025 1.8339964 27.730465 220-229
CCTGC 6139915 1.8331853 49.316692 20-24
GCCGA 6829220 1.8304243 19.655529 220-229
GGTGG 6962860 1.8260915 17.76903 40-44
CACAG 5620090 1.8234221 16.030203 140-149
TTCCA 4074395 1.8211992 22.714373 180-189
TAGTA 3665480 1.8190683 17.636787 120-129
TGTCT 3924995 1.792492 49.12235 30-34
GCTGA 5396295 1.7888069 26.283478 200-209
ATACT 3450960 1.788248 26.884323 130-139
TCAGC 5157645 1.7852086 11.937558 45-49
GCAAA 4714565 1.7732872 32.89075 200-209
CAAGG 5695745 1.7698023 17.26142 80-89
TTCTG 3834575 1.7511984 42.365417 30-34
ACCTG 5041550 1.7450248 30.256403 25-29
AGTCA 4325855 1.7358131 28.665073 160-169
TAGTG 4231925 1.7349735 25.875994 120-129
CATGA 4319130 1.7331147 23.599012 60-69
AGTTC 4045015 1.7315886 21.424706 190-199
CCTGG 6055400 1.7314786 35.24396 2
TGACC 4970660 1.7204877 14.14234 210-219
TACAA 3532860 1.7160136 17.164207 130-139
CTGAA 4275935 1.7157822 22.82072 200-209
GTGCA 5159315 1.7102509 51.362244 25-29
AACAC 4312920 1.6938641 23.030777 190-199
CTCAG 4890430 1.6927178 77.53907 6
ATGCA 4214105 1.6909719 16.821373 60-69
GGATG 5321050 1.689256 22.590311 100-109
AGGGG 6686770 1.643833 33.56769 90-99
AGCTA 4087965 1.6403563 25.454063 50-59
ATTGG 3994440 1.6376112 19.785093 100-109
TCTGC 4420085 1.6321561 33.98052 200-209
CGATT 3801610 1.6273918 35.913296 160-169
GGAGC 6325980 1.6238235 12.992292 200-209
GGGGC 7649870 1.6221905 29.169474 90-99
TCTCA 3601550 1.6098439 26.635063 100-109
GGGCC 7238380 1.6027244 28.042578 2
GCCGT 5581185 1.5958816 29.099386 230-239
TCTGA 3721390 1.5930514 21.461012 220-229
ACAAT 3184605 1.5468558 23.142916 180-189
GCACT 4460190 1.5437994 25.460163 25-29
CAGCT 4454240 1.54174 12.506604 50-59
GTCCG 5357860 1.5320243 35.261616 70-79
AGTGA 3984605 1.5312517 85.347206 9
CTCCC 4900805 1.5278515 13.673045 190-199
GTTCT 3336595 1.5237777 22.548277 190-199
CCATG 4401495 1.5234834 15.707748 160-169
AGTTA 3065705 1.5214177 16.089018 50-59
CGTCC 5095495 1.5213543 18.653662 180-189
CGGCC 6533790 1.5106101 31.598633 230-239
GGCTT 4271060 1.5104175 21.2657 25-29
TTCTC 3137700 1.4962331 21.221685 190-199
TTAGT 2797525 1.4811043 25.383043 110-119
GAGCA 4765565 1.4807734 7.716595 200-209
CATCA 3512775 1.4718076 31.334408 45-49
ACTCC 4054985 1.4655358 17.360735 140-149
TACGC 4186985 1.4492353 25.23333 140-149
CTTTA 2615915 1.4461222 41.75629 45-49
GGGGT 5420595 1.4216144 120.31014 7
CTGGT 4019150 1.4213322 31.298046 35-39
GTTAC 3316495 1.419724 15.107527 50-59
GTGAC 4276250 1.4175256 14.589576 210-219
CTCAA 3341080 1.3998697 18.50673 150-159
GGCAG 5410750 1.388892 13.616353 150-159
GAACT 3456575 1.3870016 15.177322 190-199
GTCCA 3975345 1.3759807 28.39163 180-189
CAACT 3270805 1.3704253 16.832935 130-139
CTACG 3955480 1.369105 16.397367 130-139
GAGAG 4592205 1.3665509 26.230434 220-229
GGCCT 4777865 1.3661808 67.939415 3
TAGCA 3392875 1.3614411 11.37569 130-139
CAACC 4012355 1.3592929 15.3831625 140-149
CGACA 4183815 1.3574268 16.466932 70-79
TTATT 1983835 1.356357 21.851456 240-247
ATACA 2786610 1.3535379 22.631407 35-39
AGCAG 4347540 1.3508832 12.660893 50-59
AACCC 3976645 1.3471951 19.999687 140-149
TGGAA 3499335 1.3447663 19.365948 120-129
ATCTA 2577810 1.3357916 20.263266 110-119
AAGCT 3323285 1.3335171 16.01957 200-209
CGTGT 3759950 1.3296686 23.437515 230-239
ACAGT 3305835 1.326515 12.140741 120-129
TCACT 2950130 1.3186681 14.008486 190-199
CCATA 3145870 1.3180792 14.07484 160-169
ATTAT 2052885 1.3156474 9.965658 50-59
CAATT 2536505 1.3143878 23.917528 180-189
CACGT 3790710 1.3120732 16.6122 180-189
ACATG 3253410 1.3054788 14.586216 190-199
ACAGA 3470140 1.3052222 20.540358 140-149
TCCGT 3523750 1.3011763 13.887172 150-159
TCAAG 3234075 1.2977204 22.65065 150-159
GTGCG 4732695 1.2960241 16.900133 240-247
GTGGC 4727640 1.2946398 14.079361 40-44
TGTGT 2954125 1.292044 15.500237 240-247
ATATT 2015130 1.2914511 26.316906 240-247
CCTTT 2689165 1.2823462 47.93304 45-49
AATTC 2464855 1.2772596 23.878944 180-189
TGTCC 3444570 1.2719383 53.09173 15-19
AAGAG 3528090 1.2708883 18.267994 150-159
CCTAC 3498820 1.2645291 17.399925 190-199
AGAGC 4067790 1.2639582 24.93026 220-229
TGCGA 3808035 1.2623178 14.00815 240-247
ACATA 2597625 1.2617424 20.884617 130-139
CCAGT 3644805 1.2615714 18.499546 190-199
TTACA 2428445 1.2583923 9.272666 120-129
AACCA 3201400 1.2573237 17.507587 190-199
CGGCT 4345020 1.2424132 21.805962 230-239
AGACC 3790470 1.2298071 97.28541 9
ACACA 3122130 1.226191 7.8338256 130-139
TGGAC 3690810 1.2234592 15.880524 80-89
CTTCT 2557805 1.2197064 23.121016 30-34
ACGTC 3499840 1.2113948 18.401691 180-189
AGTCG 3648145 1.2093163 15.434335 150-159
GATTG 2948110 1.2086444 23.096548 100-109
ATCCG 3475315 1.2029061 23.455898 70-79
TACCA 2837905 1.1890458 10.621534 45-49
TGAGT 2899840 1.188855 20.988413 90-99
AAGGC 3779835 1.1744838 28.835678 25-29
GTCGA 3538600 1.1730033 12.045899 150-159
ATTAG 2356995 1.1697061 25.449986 110-119
ACGCA 3597790 1.1672926 21.383875 140-149
GAAGT 3022395 1.1614821 13.646551 140-149
GGGTG 4403865 1.1549652 14.546639 60-69
GATCC 3334805 1.1542715 23.31003 70-79
GGACT 3470480 1.1504226 12.765397 90-99
ATCAT 2219510 1.1501248 15.469986 110-119
AGGCC 4266280 1.143484 13.812903 2
ATTCC 2549445 1.1395673 22.14979 180-189
CTTCG 3085660 1.1394078 102.90942 3
TCGGA 3433185 1.1380597 91.76492 5
GATCT 2638490 1.1294838 9.392462 220-229
CGCTG 3929145 1.1234981 12.195537 190-199
CCGAT 3242975 1.1224865 24.308605 160-169
GCAAG 3601615 1.1191068 17.093885 25-29
TCGAG 3373685 1.1183363 11.6850195 150-159
ACAAG 2948865 1.1091553 17.56182 80-89
AGCTC 3198985 1.1072602 12.179001 210-219
AGGTC 3334315 1.1052855 17.955791 10-14
TTGGG 3259570 1.1039566 14.575639 100-109
GAACC 3390095 1.0999066 10.201292 190-199
CGCAG 4070825 1.0910965 16.700102 140-149
CTTGA 2544455 1.0892295 22.292416 90-99
GACCC 3887635 1.0880177 41.8593 10-14
GCTTC 2941445 1.0861549 26.59827 2
TTGAG 2645255 1.0844822 20.718279 90-99
GTATA 2180800 1.0822659 10.191226 240-247
TTCGG 3054335 1.0801349 98.70222 4
CACAT 2572865 1.0779974 13.853771 130-139
ATGGG 3395415 1.077931 20.046751 100-109
GCCCC 4457330 1.0760483 12.385291 80-89
ATCAA 2186260 1.0619305 12.271888 110-119
GCACC 3780865 1.0581363 10.976832 130-139
CTCCG 3535685 1.0556442 10.06961 140-149
ACACC 3110055 1.0536145 13.234904 40-44
TAGAC 2624500 1.0531194 16.697437 170-179
CAGCA 3243725 1.0524173 12.621538 45-49
ATGAG 2734080 1.0506852 20.303602 60-69
CTATA 2025355 1.0495157 13.43512 50-59
TACAG 2612540 1.0483203 8.6994915 120-129
CCCCT 3352285 1.0450922 9.473739 70-79
GAAGC 3347420 1.0401223 12.5025625 200-209
TTTCA 1879395 1.0389614 7.914693 100-109
GACAG 3331350 1.0351291 15.791173 70-79
GAGCC 3860465 1.034714 18.281797 220-229
AAAAA 1959970 1.0345314 5.353979 190-199
CGTGA 3114455 1.0324044 20.798416 150-159
CGAGG 4018870 1.0316086 14.996954 220-229
AGATC 2568280 1.0305603 12.43988 210-219
TGGCT 2906445 1.0278351 30.787668 40-44
GGAGA 3449205 1.0264163 81.94527 7
ATGGT 2496475 1.0234866 9.424978 120-129
GGGAG 4159985 1.0226642 14.438574 5
GCCAA 3148375 1.0214813 15.560914 180-189
AACTC 2432990 1.0193915 17.075872 190-199
TGAGG 3205245 1.0175583 12.126159 220-229
AGCCG 3790415 1.0159386 19.340677 220-229
ACCGC 3617280 1.0123544 11.255108 220-229
AAACT 2062230 1.0016854 16.761307 130-139
GCTCT 2709675 1.0005717 16.65138 210-219
GTAGC 3011130 0.9981535 11.33364 50-59
TTATA 1557060 0.9978844 13.0894985 110-119
ACTCA 2378045 0.9963703 12.985833 190-199
TAGTT 1878300 0.99443555 14.489397 50-59
ATGAC 2473965 0.99271506 11.030064 160-169
CCGTC 3301220 0.98564035 10.315818 140-149
ATGAT 1980280 0.9827538 8.93561 120-129
CCGAG 3631990 0.97347623 18.13018 220-229
AAGGT 2529490 0.97206265 20.708408 15-19
AACTG 2407175 0.9659145 12.511109 60-69
GATAC 2404645 0.9648993 24.42808 35-39
TTTAT 1407140 0.9620681 11.620845 240-247
GCAGT 2885345 0.9564573 6.5064373 50-59
TTTAG 1801620 0.9538386 29.760292 45-49
GGGAC 3715780 0.95380807 9.800024 90-99
CGAGT 2865710 0.94994855 14.939448 160-169
GTAGA 2466270 0.9477677 16.336763 170-179
ACAGG 3042465 0.94536567 15.316053 70-79
CGGAC 3513100 0.9416103 10.600316 220-229
GCCAT 2711030 0.9383651 7.7347083 60-69
CATAC 2230580 0.9345844 14.011166 130-139
ACCAA 2378255 0.9340403 16.371864 130-139
CAGAA 2480110 0.9328427 18.230352 140-149
GGTAT 2263760 0.92807966 9.22568 110-119
TAACA 1907700 0.92662567 8.857873 120-129
CCAAC 2725710 0.9234073 13.424041 130-139
TATTT 1348430 0.9219277 11.527296 240-247
AGCCC 3275535 0.91671157 11.497373 80-89
AAGTT 1841405 0.9138343 17.674288 140-149
TACAC 2176850 0.91207224 22.100481 35-39
TCATA 1747825 0.9057029 8.8355665 110-119
TATGG 2208435 0.9053979 11.752076 35-39
GAGAT 2353980 0.9046156 12.078618 210-219
GTTAT 1696290 0.89807326 10.514817 110-119
AATCA 1847595 0.8974309 13.311918 110-119
CCCAG 3188115 0.89224565 14.416433 80-89
GATGA 2321470 0.8921222 7.0688577 60-69
AGTCT 2056410 0.88030726 15.769467 8
TGGTT 2001175 0.8752528 8.883972 50-59
GACTG 2633775 0.87306476 12.394473 90-99
GTTTC 1909250 0.87192863 5.546671 100-109
CCCCA 2961730 0.8654971 16.645504 80-89
AGAAG 2392090 0.8616785 15.804921 140-149
CGTAT 2003925 0.8578395 7.3111887 240-247
TCCTT 1794925 0.8559219 10.201244 45-49
GCCTA 2467585 0.85410184 16.726631 190-199
CGGAG 3321765 0.8526678 70.92991 6
GTCTG 2411050 0.85264367 14.13756 1
ATAGT 1703525 0.8454085 15.552936 120-129
ACGCT 2439635 0.8444275 15.02372 190-199
TTATG 1589895 0.8417441 12.259569 50-59
CATGG 2537910 0.84128666 8.666366 200-209
TCTTC 1752865 0.8358654 45.51199 2
GGTTC 2347415 0.8301399 11.223438 160-169
GTCTA 1937195 0.82927376 9.554621 30-34
TTACC 1853765 0.82860774 9.747541 45-49
GATCA 2061000 0.8270067 9.22164 110-119
ATATA 1366100 0.820661 11.2001705 130-139
CTATC 1834680 0.820077 8.171224 110-119
GGTAG 2580320 0.81916547 11.698022 120-129
TGGCA 2470740 0.8190206 5.77996 100-109
ATCCA 1952355 0.81801176 9.567394 180-189
GGTGC 2956905 0.8097332 11.665562 60-69
GCTTG 2289130 0.8095279 16.907085 90-99
TCATC 1807490 0.80792344 10.465195 110-119
ATATG 1613810 0.8008857 5.6228166 50-59
ACATC 1894245 0.79366434 5.6065817 110-119
TGATG 1935260 0.7934036 12.327654 120-129
ACAAA 1740660 0.79252803 12.803808 130-139
CATTA 1517445 0.7863226 12.776237 110-119
TATAC 1503555 0.779125 9.293574 130-139
CCTCC 2461725 0.7674556 10.193552 140-149
CTGAC 2201360 0.7619538 9.502453 220-229
GTTCA 1774805 0.7597579 13.721302 160-169
TCAAC 1806230 0.75678724 7.6006546 110-119
AACGC 2309965 0.74946153 15.23352 180-189
ACGCC 2663515 0.74542785 13.653257 180-189
TATGA 1501105 0.7449535 5.047331 50-59
CCAGC 2658170 0.74393195 6.5740056 80-89
TGACT 1732070 0.7414639 5.1661696 2
ATCTT 1339880 0.74070835 12.740796 3
TTGGA 1797425 0.7368951 6.5966616 60-69
GGGAT 2320280 0.7366115 13.226104 100-109
TATTG 1384535 0.7330196 6.8288813 240-247
GCGGA 2847495 0.73092693 8.857454 220-229
CAACG 2252830 0.73092425 15.55186 180-189
TTTAC 1307810 0.72297966 13.322976 45-49
GACCG 2687760 0.72039586 9.014148 210-219
TTTCT 1220890 0.7200315 9.80584 4
GGTTA 1745285 0.7155191 7.882502 50-59
ATAAA 1269175 0.7146761 7.6557713 130-139
GGATA 1845075 0.7090476 15.963609 35-39
GATTA 1428275 0.7088102 5.7674847 50-59
GACCA 2179340 0.7070806 6.1714344 170-179
GGGCA 2748430 0.70549786 13.606114 150-159
CCCCC 2796165 0.7048382 13.174109 80-89
ATGCC 2032125 0.70337665 8.043557 50-59
AATAC 1442130 0.70048463 11.507925 130-139
GAAAT 1505770 0.7004597 6.9810147 110-119
TGCCA 2023375 0.700348 7.410234 60-69
CGCCG 2996930 0.6928892 10.799706 220-229
TCCGG 2412750 0.68990076 11.308126 70-79
TATAG 1387860 0.6887534 6.6262136 120-129
CATCC 1893380 0.6842975 6.463134 110-119
CAGTC 1974640 0.6834794 5.7118583 210-219
CACAA 1735780 0.68171346 8.38027 130-139
AAATA 1187715 0.6688057 12.776215 130-139
GCCGC 2879155 0.66565967 11.900306 220-229
CAAAC 1691890 0.664476 13.544442 130-139
CCCGT 2224940 0.66429704 10.099509 140-149
AGGCA 2133850 0.66303754 6.5476727 80-89
AGATG 1720385 0.66113013 6.042696 120-129
GCGAC 2433195 0.6521652 7.2900567 70-79
CACGC 2314445 0.64773494 12.211662 190-199
GACGA 2080220 0.64637345 9.832317 220-229
TACTT 1161600 0.6421522 11.489846 1
ACTTC 1426495 0.63762385 29.528566 4
CACCG 2224905 0.6226757 9.058178 45-49
AAGCA 1651710 0.62125695 5.8818316 120-129
TCAAT 1193310 0.6183596 8.058913 110-119
AATAA 1095375 0.6168088 7.1634474 130-139
AATGG 1590680 0.61128557 6.7302537 120-129
CCCTA 1671830 0.6042258 11.905782 110-119
AGTAA 1297395 0.60352707 5.561525 50-59
TAGCT 1404075 0.601056 12.458014 50-59
CCGGC 2579490 0.59637725 9.189174 70-79
TGATT 1120780 0.5933788 6.1666355 50-59
ACGAC 1828765 0.59333754 9.16121 220-229
TCCAC 1637075 0.5916649 10.091495 180-189
ATTTC 1064600 0.58852893 6.7726965 240-247
CACGA 1810305 0.5873483 8.648843 180-189
TTCAA 1133405 0.58731747 5.742289 200-209
GGCCC 2539655 0.58716744 10.143823 70-79
GGTGA 1843630 0.58529097 6.753191 120-129
GTTCC 1578025 0.5826999 6.855549 140-149
ACCTA 1389955 0.5823733 7.4360895 130-139
ACCCG 2074360 0.5805433 8.781188 140-149
CTTTG 1269240 0.57964474 7.3750105 45-49
ATTAA 957200 0.5750214 7.068688 110-119
CCGTA 1650725 0.57136315 6.239962 230-239
GGGGA 2314385 0.5689537 9.221939 4
TCAGG 1675165 0.55529714 5.3666196 150-159
TTAGC 1291565 0.5528927 9.4915495 45-49
CGGTT 1556655 0.55049545 11.108411 160-169
TAATA 910630 0.54704523 7.7919292 130-139
CTGTT 1183030 0.54027385 5.860862 240-247
ACATT 1041605 0.5397478 6.615221 110-119
CGGCA 1998700 0.5357082 10.60869 70-79
GAAGA 1487080 0.5356758 6.437778 15-19
AAGAT 1147090 0.5336076 5.816684 15-19
GGAGG 2166055 0.5324892 5.3388224 6
TATGT 994765 0.52666223 6.928919 140-149
CTGCG 1838575 0.52572143 11.618304 25-29
CCACG 1877850 0.5255467 7.590795 180-189
GCTAC 1517770 0.52534366 7.842569 50-59
GTCCT 1408600 0.52013814 6.05556 45-49
GGGTT 1517205 0.5138495 6.3897347 100-109
AAATC 1039010 0.5046775 7.480264 110-119
GTAAC 1232850 0.49469927 6.94544 120-129
AGTAT 991800 0.4922007 5.3804216 110-119
TGACG 1476510 0.48944533 8.614617 220-229
ACCCC 1665885 0.48681638 8.702507 140-149
TGGCC 1700965 0.48637325 14.854639 2
CGCGG 2193020 0.4855792 6.382088 220-229
GGTTT 1109425 0.4852286 5.8705554 100-109
TATCC 1085545 0.48522386 5.375836 110-119
AACAT 994790 0.48319858 6.0778203 110-119
CATAA 981325 0.47665825 6.272163 110-119
CAATC 1093500 0.45816246 5.985269 110-119
ATTCT 823150 0.45505127 7.437014 2
CCGGT 1590030 0.4546525 8.633537 160-169
ATGCT 1056855 0.45241815 6.9168057 50-59
CCGCG 1931025 0.44645235 6.626171 220-229
TAAAT 729845 0.43844178 7.6885905 130-139
TGCTA 1022535 0.4377264 6.072516 50-59
GCCTT 1163985 0.4298119 8.956985 1
TGCGC 1488845 0.42571974 8.618719 25-29
GCATG 1279710 0.4242085 8.536135 60-69
GGTCA 1268425 0.42046764 5.481532 160-169
CACTT 928665 0.41510063 6.713204 3
CTAAC 972285 0.40737495 5.682485 110-119
ATGGC 1227735 0.40697947 7.08053 1
TACCT 902715 0.40350136 5.0321417 2
CCTAA 945405 0.3961126 7.8058047 110-119
AGCTT 920605 0.39409226 7.053312 1
TTTGA 740790 0.39219925 6.176457 45-49
CATGC 1131400 0.39160994 7.0704103 60-69
ACGAG 1259255 0.39128023 7.454025 180-189
CCGGG 1767045 0.39125967 5.33203 80-89
GCGCT 1367305 0.3909666 7.73522 25-29
GGTAA 982375 0.3775188 7.326545 120-129
GAGGC 1457795 0.37420318 5.6469703 35-39
TTCTT 608230 0.3587094 19.377676 1
TGGGC 1254420 0.34351644 9.147895 1
TGCTG 969560 0.34287515 14.011309 1
ATGTG 824000 0.33781746 5.2210984 140-149
CGAGC 1210250 0.3243813 6.275403 180-189
GCTTT 679690 0.31040522 5.112277 45-49
CTCTT 643705 0.306955 8.727635 1
GCGAG 1162195 0.29832524 5.31655 240-247
CGGGA 1161305 0.2980968 5.0686307 80-89
AACTT 568185 0.29442695 10.5011635 3
GACTT 686785 0.2939987 6.6656914 1
CTTTC 614710 0.29312858 5.113523 45-49
CGCTT 791095 0.2921189 5.68775 1
CTCGG 1011155 0.28912926 5.135879 6
ATTGT 542965 0.28746402 5.589883 240-247
GAGGT 889690 0.2824469 6.713104 7
TTCCT 578290 0.27576143 6.2557178 4
CCCTT 699330 0.26963913 5.4245005 3
AATCT 508540 0.2635196 7.588842 2
TTGGC 745120 0.26350424 7.316042 1
TAACT 504255 0.26129916 6.984602 2
GTCTT 568135 0.25945956 11.517158 1
TGTAA 510630 0.2534104 7.9507637 25-29
CGGGG 1185655 0.25142363 9.100724 3
CCTCG 800830 0.23910262 5.610102 5
TGCTT 458925 0.20958483 10.928184 1
TCGGT 561345 0.19851404 6.1019382 7
TCGCA 470420 0.16282585 5.195434 7
TAGGC 454655 0.1507127 6.70175 1
TTGCT 271060 0.12378943 5.683095 4
Produced by FastQC (version 0.10.1)