FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11853962
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13662635 8.680266 60.71692 110-119
ATACA 12497535 7.5100117 36.572773 110-119
CACAG 18294615 7.489676 41.737495 110-119
TCCCT 21043780 6.8944054 102.411026 30-34
CAGTA 15135835 6.551316 38.9866 110-119
AGTAA 11816405 6.3779736 47.3372 110-119
GGAGA 18861925 6.2299976 95.446014 35-39
GGTGA 21010435 5.8836136 102.09689 45-49
GTAAT 12819355 5.866387 39.296394 110-119
CCCTG 20739345 5.7725368 67.35656 30-34
AATAC 9043755 5.434568 38.53421 110-119
AGACG 14636375 5.3821325 103.11931 40-44
GTAGT 16062060 5.294337 30.361809 80-89
ACAGT 12162745 5.264459 35.440678 110-119
GTGAC 16074375 5.0114346 84.82639 45-49
GAGAC 13064935 4.8042774 143.33263 40-44
CCCCA 13073915 4.778473 101.802315 60-69
TAGTA 10308330 4.7172923 39.07997 80-89
CTCAG 13304805 4.6180162 34.018795 140-149
ACTCC 11702865 4.5222864 116.12554 30-34
GGCCC 19034860 4.5011144 59.034843 60-69
ACCAG 10813060 4.4267845 91.41299 45-49
GCACA 10576595 4.3299775 34.02678 110-119
GTGTC 16323875 4.3147774 23.444233 120-129
TGACC 12350985 4.286951 145.18437 45-49
CGTGT 16172165 4.2746773 22.914286 120-129
AGTAG 10961280 4.2615194 31.764101 80-89
CACTC 10807370 4.1762443 94.87385 25-29
CTCCC 13097745 4.058702 66.56111 30-34
CCCAG 12188025 4.0012703 87.197586 60-69
GACCA 9564235 3.915525 104.78863 45-49
ATGCA 9003035 3.8968265 93.08671 25-29
TGAGG 13493940 3.778747 69.62566 35-39
CCCTT 11500705 3.7678835 39.755497 20-24
AGGAG 11342805 3.7464707 95.77151 35-39
GACGG 13992765 3.706212 109.76088 40-44
ATGGT 11182375 3.6859071 25.504862 190-199
ACGGT 11791150 3.676073 97.11871 40-44
TACAC 7614680 3.6693802 26.430374 110-119
AGATA 6797485 3.6689823 19.714708 150-159
CTGAG 11654085 3.6333408 105.07784 35-39
CCACT 9357185 3.6158555 26.760035 240-247
TGTCC 12241220 3.602291 20.300724 120-129
CAGGG 13559220 3.5913801 34.84621 50-59
GAGGA 10696695 3.5330637 126.024376 35-39
TGGTG 14626415 3.4725947 18.21356 25-29
GCCCC 13165820 3.4660697 73.31637 60-69
TGCAC 9922610 3.444077 111.84027 25-29
CTTGG 12891395 3.4074936 22.841368 20-24
AGGCT 10870915 3.3891754 28.747465 30-34
CCGTG 13416660 3.3542633 23.127151 120-129
GGGTT 14061730 3.3385274 41.082127 50-59
GGATG 11741885 3.288114 68.29677 20-24
TCTTG 10551470 3.2828376 17.964314 170-179
CCTGA 9443740 3.277864 81.798164 30-34
CAGAT 7529635 3.2590878 18.054863 150-159
CTCTC 9927325 3.25241 13.61189 130-139
TCAGG 10372370 3.2337465 20.820684 140-149
CCAGG 10961005 3.2321832 39.41503 50-59
CTTCA 7813945 3.1924095 16.86655 200-209
GCACT 9122515 3.166369 86.48987 25-29
TCGCA 8980420 3.1170487 23.423502 100-109
TCTCA 7609365 3.1088278 21.499744 130-139
CCTGG 12433370 3.1084332 28.75345 50-59
TGTCT 9963645 3.0999498 19.451849 180-189
ACAGA 5990405 3.0582373 14.05814 140-149
CGCAC 9260720 3.0402498 24.016636 100-109
GCCCT 10618685 2.9555786 21.42261 20-24
CGGAT 9480105 2.9555693 77.27006 20-24
GGTTG 12434020 2.9520776 71.60585 15-19
TGGCC 11620370 2.905178 43.174625 60-69
TTCCC 8849700 2.8993564 48.001984 50-59
GATGG 10208720 2.8587773 22.246843 15-19
ACCAC 6260680 2.8535154 18.742083 230-239
TCTCT 8234575 2.8523092 15.221201 180-189
GATGC 9122395 2.8440475 93.35796 25-29
GGTTC 10739380 2.8386664 46.215347 50-59
AGGGT 10113215 2.832033 48.915146 50-59
GACTC 8054625 2.795711 18.388906 190-199
CGGTG 12373050 2.7785015 68.62697 40-44
TCAAA 4574410 2.748852 56.55097 70-79
GTTGG 11543325 2.7406092 51.49285 15-19
CGGCC 11527945 2.725978 12.399349 120-129
GCCGT 10895245 2.7238915 21.920362 120-129
TTCTT 7368775 2.698566 20.164999 170-179
TCACA 5599615 2.6983562 11.278854 140-149
TCCAT 6560605 2.6803539 19.839525 70-79
AGATG 6887230 2.6776128 17.234024 180-189
GTTCC 8959855 2.6366658 52.189796 50-59
TGGAG 9338305 2.6150327 19.509697 25-29
AGTCA 6020400 2.6058385 30.361765 70-79
CCAGT 7464775 2.5909777 34.796474 60-69
GGCTG 11535970 2.5905259 14.950148 30-34
TGTTC 8054280 2.5058963 12.995789 140-149
AGGGA 7543740 2.491659 8.768335 150-159
TCCTC 7604510 2.4914048 16.89915 130-139
ACAGC 5991780 2.4529893 11.486579 120-129
GCGGA 9255055 2.4513521 85.18403 20-24
GAGAT 6281090 2.441958 16.780659 180-189
AATAA 3244395 2.431225 10.721217 110-119
GGCGG 12502290 2.385185 41.24878 20-24
ACTCT 5821790 2.3785088 13.040606 200-209
CTGGA 7583655 2.3643215 14.814419 180-189
GTTCT 7520415 2.3397973 17.057661 160-169
CACGG 7878925 2.323339 9.346708 120-129
TACCA 4807830 2.3168087 15.567817 230-239
TTGGG 9676660 2.297427 77.47777 15-19
CATTT 5308540 2.2930086 17.882076 150-159
TGTAG 6955995 2.292818 7.6011257 210-219
CTGGC 9113705 2.278493 33.898434 60-69
TCATT 5266065 2.2746618 16.528584 150-159
GTGAA 5828870 2.2661443 18.288973 190-199
CAGCT 6501220 2.2565336 11.693038 150-159
TTCAT 5204575 2.2481012 16.00088 140-149
CAGAG 6059555 2.2282379 9.066886 140-149
TTCAC 5427110 2.217261 18.583157 200-209
TTGTC 7046840 2.1924558 13.210689 170-179
GATAC 5058040 2.1892955 14.6335745 150-159
ACTAC 4536560 2.1860883 12.398956 230-239
ATTTG 5540850 2.1497502 15.759349 150-159
CTACT 5245230 2.1429536 7.6633677 230-239
GAATC 4935575 2.1362884 14.878002 190-199
CACTA 4382450 2.1118257 14.613461 240-247
TCAGC 6067780 2.1060894 13.71126 140-149
TGAAT 4548465 2.0814662 14.346576 190-199
CTCTG 7068510 2.0800893 14.666644 180-189
TCTGC 7056845 2.0766566 11.974723 210-219
TACAG 4789990 2.0732741 13.833199 160-169
CTCGC 7432320 2.0686934 15.973472 100-109
ATGTA 4516455 2.0668178 8.724789 70-79
GCTGT 7804800 2.0629892 10.197038 140-149
TTGGA 6243275 2.057893 15.447976 170-179
GTCTC 6984780 2.0554495 11.966403 180-189
GTCCT 6973935 2.0522583 14.335112 130-139
ATATA 3229315 2.051677 10.732672 110-119
ACACA 3599550 2.0458891 13.766632 110-119
TGGGG 10121835 2.0416164 51.27896 15-19
CAGGC 6876250 2.02767 15.360014 140-149
GTTCA 5516095 2.0242352 15.059066 140-149
AATCG 4676290 2.0240607 13.451276 190-199
TAGTC 5451410 2.0004978 24.784494 70-79
TCTCG 6796155 1.9999418 15.522864 100-109
AATAT 3140080 1.9949834 13.360192 110-119
TTGCA 5433970 1.9940982 14.259118 150-159
GAACT 4580145 1.9824461 10.397311 160-169
ATCTC 4850135 1.9815364 7.94109 100-109
TTTGC 6355145 1.9772514 12.161382 150-159
AGCTC 5670050 1.9680396 11.712178 140-149
TGGAA 5056840 1.965995 9.090676 170-179
GAGTC 6266575 1.9537015 12.375462 210-219
ACGGC 6602035 1.9468093 14.156458 120-129
CCTTC 5919780 1.9394499 13.845131 200-209
AACCA 3409320 1.9377675 14.67492 70-79
GATAT 4228610 1.9350944 7.7375116 180-189
GTCAA 4452025 1.9269912 38.779076 70-79
TCGGC 7672240 1.9181162 7.8451953 130-139
CCAGA 4679820 1.9158827 29.658476 60-69
TACTA 3751700 1.9113998 10.152108 230-239
CAGCC 5786010 1.8995193 10.45888 120-129
ATATG 4143015 1.8959242 7.950787 180-189
ACACG 4614965 1.8893315 12.9937 120-129
CATAG 4326390 1.8726119 8.134835 210-219
GACGT 5906470 1.8414334 9.303561 60-69
CCTTG 6221345 1.8307891 25.899204 20-24
AGTAT 3987280 1.8246571 11.896801 220-229
GGGGC 9556920 1.8232678 46.76501 15-19
AAAAA 2053855 1.8153238 8.100526 8
CTGTT 5813945 1.80887 12.717647 140-149
GTTGT 6435775 1.7985327 6.6766596 210-219
CGTAG 5699485 1.7769026 9.676331 210-219
GGGCG 9312430 1.7766241 57.666996 20-24
GCAGA 4802805 1.7661022 14.562711 150-159
ATTGT 4478540 1.7375932 8.103097 50-59
TGCAG 5561860 1.7339957 13.5036745 150-159
AGAAC 3395025 1.7332371 11.088608 160-169
AGTCT 4706460 1.7271247 13.011869 210-219
CACCA 3738300 1.7038558 13.075761 230-239
GTCCC 6068040 1.688963 22.230007 50-59
ATAGT 3683885 1.6858175 7.26453 220-229
CAAAA 2366830 1.6775529 22.144417 70-79
GGTCA 5376895 1.6763302 7.3637605 130-139
CTCCA 4323955 1.6708869 7.506064 150-159
TCCCA 4303170 1.662855 10.564923 240-247
GTCAC 4776670 1.6579529 8.242281 140-149
TTGGT 5911065 1.6518981 27.277151 25-29
TCAGA 3802700 1.6459408 9.992075 130-139
TGACT 4477445 1.6430832 11.186602 190-199
AATAG 3007830 1.6234937 9.214869 240-247
AACTG 3735220 1.6167331 10.28661 160-169
TCTGG 6111575 1.6154305 11.992294 180-189
GAGAA 3503165 1.6064109 10.289442 160-169
TGGTT 5739170 1.6038606 17.459711 10-14
CTGTG 6011210 1.5889018 5.197342 210-219
ATAGA 2935395 1.5843965 9.823159 240-247
GTCTG 5983110 1.5814743 8.94873 210-219
TTCAG 4276540 1.5693574 9.062538 150-159
TGTGC 5916275 1.5638081 7.7873363 220-229
CAGAC 3815300 1.5619547 17.8437 60-69
GCTCC 5610015 1.5614777 6.080419 150-159
GAACC 3812225 1.560696 12.093791 70-79
ACCAT 3234975 1.5588776 12.4063425 45-49
TTGTA 3998770 1.5514511 8.476012 210-219
ACTGA 3582200 1.5505008 8.28645 180-189
GGCCG 7281460 1.5465713 12.829554 120-129
TGCTC 5243965 1.5431705 5.2325797 150-159
GCTGA 4921935 1.5344894 24.36498 30-34
CTGGT 5803655 1.5340401 6.6309285 160-169
TAGTT 3939815 1.5285776 9.217539 220-229
GGACG 5759850 1.5255901 6.9763856 170-179
GTGCT 5765545 1.5239669 7.5165443 220-229
CCCAC 4158970 1.52009 10.381325 240-247
CCACC 4148685 1.5163308 9.437003 230-239
CAAAG 2955580 1.5088906 27.698765 70-79
GCTCA 4336170 1.5050582 10.510168 140-149
CCTCG 5371185 1.495002 9.717596 130-139
GCTCT 4991270 1.4688085 9.825665 130-139
CATGT 3974705 1.4585931 8.706051 70-79
CGACT 4089010 1.4192703 8.124159 190-199
CCATC 3639035 1.4062161 10.764322 240-247
GTGGA 4998655 1.3997879 24.553202 25-29
CACTG 4017830 1.3945642 6.9003625 230-239
TCGAC 4013440 1.3930404 8.819958 190-199
ACCGT 4012630 1.3927593 20.91733 45-49
AATTG 3035515 1.3891107 9.06164 170-179
TATAC 2716040 1.3837563 6.7499995 110-119
CTCTT 3974395 1.3766593 7.7438707 200-209
GCGTA 4414350 1.3762419 10.252409 210-219
CCTCA 3560200 1.3757522 7.3064065 130-139
TGGAC 4408505 1.3744195 9.97582 170-179
TGATA 2994230 1.370218 7.230655 180-189
ACGGA 3702795 1.3616028 9.430265 200-209
GGGAG 5643445 1.3426167 6.294744 150-159
GTCCA 3865275 1.3416133 18.285742 70-79
GAGGG 5602030 1.3327638 7.8165226 200-209
CCATG 3831860 1.3300151 8.845806 70-79
ACTTC 3245665 1.3260256 6.6760745 220-229
GTAGG 4732735 1.3253214 5.0401306 160-169
GGAGG 5532025 1.3161091 18.71083 30-34
CTCGA 3748715 1.301156 7.84482 190-199
GAATT 2841255 1.3002137 10.071861 170-179
CTGCT 4406575 1.2967471 5.430167 90-99
AGCCG 4393095 1.2954367 9.310619 120-129
TATGG 3927280 1.2945004 5.909268 190-199
TCCAC 3345265 1.292696 9.182615 240-247
GTGTT 4599070 1.2852498 7.561327 160-169
CTTGA 3492610 1.2816793 7.9370623 200-209
GGAGT 4565535 1.2785 7.012299 210-219
AGATC 2951145 1.2773582 6.6824255 130-139
CTGAT 3476440 1.2757454 6.8712144 180-189
ATCGG 4087495 1.2743397 7.9952283 190-199
TTGGC 4799415 1.2685963 8.85553 60-69
ATAAA 1683640 1.2616552 5.554009 7
GTGGT 5312090 1.2611933 11.968371 50-59
TGGTC 4748255 1.2550735 13.509605 50-59
GGCTC 4984360 1.2461265 8.280306 130-139
ACGTG 3991700 1.244474 8.875044 170-179
ACTAT 2435250 1.2407005 6.1949005 230-239
AAGTA 2283060 1.2322949 15.44865 70-79
ATAAC 2041640 1.226861 7.3318853 80-89
TTCCA 2995415 1.2237853 7.4454603 230-239
TGCCC 4364665 1.21485 7.653809 60-69
GGAAT 3118475 1.2123986 8.456446 170-179
ACTCG 3450455 1.1976318 8.075253 190-199
GATCT 3262215 1.1971316 5.2821484 130-139
GCATA 2744250 1.1878068 7.7709417 210-219
AAAGT 2195385 1.1849718 20.27745 70-79
TTGAG 3587750 1.1825855 7.6355724 200-209
AGTTC 3212765 1.178985 5.514831 160-169
ATACC 2440615 1.1760894 8.17668 70-79
ACTGG 3765100 1.173828 6.846426 160-169
CCATA 2425360 1.1687384 11.271372 70-79
TCACG 3336205 1.1579763 9.462396 200-209
TATCA 2270775 1.1569045 7.212535 70-79
ACTGT 3147165 1.1549116 5.238544 230-239
CAATA 1916225 1.1514969 6.687959 100-109
AAAAG 1782255 1.1346449 14.999642 70-79
ATGGG 4049415 1.1339694 34.978096 15-19
AGCTT 3059975 1.1229157 5.8297343 150-159
TCTGT 3603725 1.1212128 6.3239813 210-219
AGAGA 2389090 1.0955409 14.237492 35-39
AGGTT 3319600 1.0941985 22.775219 10-14
CTCGG 4368230 1.0920895 8.336002 130-139
CGGCT 4365690 1.0914546 7.8361187 130-139
AGAGC 2965305 1.0904111 6.6005898 140-149
GAAGA 2376645 1.0898341 19.380434 35-39
TCTAC 2619830 1.070339 6.888964 180-189
GTCTA 2913820 1.0692813 7.10827 180-189
TATGT 2749110 1.0666053 7.4398527 220-229
ATATC 2089705 1.0646538 7.677486 70-79
ATCCA 2206360 1.0632061 14.634889 240-247
CTGCC 3766310 1.0483053 5.6806526 200-209
CTGAA 2411140 1.0436252 15.704882 35-39
AACTT 2041050 1.0398653 5.698051 200-209
CTGCG 4152500 1.0381554 6.8497458 210-219
TACTG 2816740 1.0336561 5.999306 180-189
GCTTC 3486940 1.0261211 5.187407 150-159
GAGCT 3288715 1.0253077 5.8812623 140-149
CATGG 3270500 1.0196289 5.2101464 190-199
AAAAT 1355275 1.0155911 5.2480693 8
GCGGT 4511880 1.0131912 5.630316 130-139
CTACC 2610115 1.0086151 8.023544 230-239
TAGAT 2198195 1.0059369 7.495646 240-247
TCCCC 3235595 1.0026395 5.263655 80-89
TATAA 1574320 1.000211 6.4420323 240-247
TGCGT 3773965 0.9975462 8.0927725 210-219
ATACG 2302635 0.9966604 5.1093163 120-129
GGGGT 4862990 0.9808854 7.4110994 10-14
ACAAT 1625235 0.9766355 7.686605 100-109
CGTGG 4331165 0.9726097 8.244696 50-59
CTTCT 2806575 0.9721473 5.1441245 210-219
CGGAG 3655695 0.9682704 6.5720024 210-219
CCATT 2359405 0.9639416 6.116957 50-59
CGAAC 2351485 0.9626801 12.678581 70-79
CAGCG 3222355 0.95020866 6.1844764 160-169
GAGTT 2877770 0.9485635 5.270938 160-169
TAGGC 3025130 0.94313097 5.6619706 160-169
ACCCA 2064435 0.94093555 12.411356 240-247
GAGGC 3545300 0.9390304 15.944025 30-34
TGAAG 2414175 0.93858135 10.470252 35-39
AAGAG 2042620 0.9366636 14.350408 35-39
GGTGG 4623085 0.9324956 24.449444 25-29
ACGTC 2676505 0.9289985 12.47175 70-79
CCCAA 2036260 0.92809385 9.066784 240-247
ACTAA 1522050 0.91462946 12.062396 240-247
TGTAA 1995730 0.9132849 6.366957 230-239
TGCAT 2465220 0.9046591 5.8834105 210-219
CATCA 1867385 0.89986 5.6947227 230-239
GGGCC 4185640 0.8890238 18.503765 15-19
ATCAA 1479250 0.8889101 13.76256 70-79
TCGAA 2042075 0.88388103 16.344099 70-79
TTCTG 2779140 0.86466295 5.1962028 210-219
CCAAT 1778295 0.8569291 10.008645 240-247
CGTCC 3044210 0.8473179 10.188636 70-79
CGGTC 3349915 0.83750343 5.314685 130-139
TGGGC 3727370 0.83702105 20.064974 15-19
TGAAC 1920095 0.8310839 6.361847 190-199
AGCGT 2662095 0.82994926 6.3124514 160-169
CCAGC 2521455 0.82778156 5.5169787 80-89
GACCG 2789355 0.8225255 19.365763 45-49
GGTCC 3273960 0.8185141 13.7426 50-59
CTTCC 2482195 0.8132217 5.2106357 230-239
GTATG 2454030 0.80889136 5.185007 220-229
AACCG 1926415 0.78865963 5.6722913 190-199
TAACC 1629530 0.7852418 5.864029 80-89
TGAGA 1987975 0.7728836 5.660519 240-247
AAAGA 1201885 0.76516145 5.818194 8
CATAC 1545300 0.74465287 9.688559 70-79
AAAGG 1620810 0.7432384 8.830952 10-14
CTAAT 1450030 0.7387549 9.389055 240-247
AGCCA 1786350 0.73131806 5.6786757 80-89
CCTTT 2096095 0.72604966 5.1745715 50-59
AATCC 1502345 0.7239536 8.823253 240-247
GTCGA 2291730 0.7144822 11.493974 70-79
GTAGC 2275330 0.70936924 5.4812527 80-89
ATGAT 1550060 0.70933765 6.5230527 240-247
AAAGC 1368880 0.69884413 10.1777 70-79
AAGGT 1760775 0.684553 9.8431425 10-14
CATTG 1858520 0.6820191 5.612404 50-59
AGGAT 1726315 0.67115563 6.599672 10-14
CGATG 2133405 0.665122 15.208505 15-19
TAGGT 1986935 0.6549286 5.330467 10-14
GGAAG 1964595 0.6488957 6.6038218 30-34
AGAAG 1397970 0.64105296 5.6826324 9
GAGGT 2274365 0.636897 6.6956553 10-14
CCGTA 1822505 0.63258034 5.3532443 80-89
TATGA 1381030 0.6319862 5.51181 240-247
CAATC 1295475 0.6242666 8.556146 240-247
ATAGG 1600350 0.62218297 5.2106967 10-14
TAAAA 786040 0.5890282 5.4766884 8
AATGG 1499790 0.5830873 6.676874 10-14
CATCC 1498325 0.5789911 7.114343 240-247
AAATG 1049940 0.5667112 5.38227 9
GGGTC 2499000 0.5611773 5.4974947 70-79
AAGAA 881245 0.561031 5.5689216 8
GAAAA 846210 0.53872645 5.9385056 8
AAGCA 1047840 0.534946 6.8599277 70-79
GAAGG 1614900 0.53339326 5.5357413 10-14
AAGGG 1555815 0.5138778 6.2482467 10-14
ACGAT 1138510 0.4927867 6.6919913 10-14
TGATC 1335050 0.4899219 5.064253 240-247
TTGCC 1655140 0.4870672 5.3392267 60-69
ATAAG 897845 0.48461705 5.798239 9
GGTCG 2150745 0.48297292 5.56663 70-79
GATCC 1357280 0.47110358 5.086978 240-247
CGGTT 1692770 0.44743827 7.843307 10-14
GAAGC 1203675 0.44261906 5.3463655 30-34
TAAGG 1137855 0.4423745 5.958909 10-14
TACCG 1253255 0.43499714 5.146823 70-79
AAGCT 976440 0.4226372 8.896537 30-34
TAAAG 750355 0.40500847 5.6775813 9
Produced by FastQC (version 0.10.1)