FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_11_SL248340.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_11_SL248340.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11974012
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13719405 8.505053 60.315342 110-119
ATACA 12522780 7.3505535 37.50391 110-119
CACAG 18198185 7.3266897 41.46358 110-119
TCCCT 21399795 7.064251 107.244095 30-34
CAGTA 15459425 6.573482 38.77801 60-69
AGTAA 11865345 6.224592 46.806694 110-119
GGAGA 18882500 6.072418 93.51531 35-39
CCCTG 21440780 5.989435 70.40419 30-34
GTAAT 12984660 5.8311625 39.19702 110-119
GGTGA 21142760 5.8204675 101.268974 45-49
AATAC 9136495 5.3628902 39.533165 110-119
GTAGT 16116275 5.2429047 27.721462 80-89
AGACG 14312750 5.1500807 99.846924 40-44
ACAGT 11981215 5.0945168 34.991974 110-119
GTGAC 16258820 5.008111 83.9945 45-49
GAGAC 13115285 4.719203 138.7744 40-44
CCCCA 12916720 4.7162013 100.81241 60-69
ACTCC 12195635 4.702913 120.779 30-34
CTCAG 13450945 4.635819 34.773373 140-149
ACCAG 11295855 4.5477734 93.89731 45-49
TAGTA 9880105 4.436966 35.104145 80-89
GGCCC 18511460 4.3759747 59.263172 60-69
GCACA 10806835 4.350891 33.645348 110-119
GTGTC 16463165 4.3410244 23.946272 120-129
CACTC 11193280 4.316382 98.77313 25-29
CGTGT 16302700 4.298713 23.502022 120-129
TGACC 12362755 4.2607784 143.68529 45-49
CTCCC 13431560 4.198175 69.8828 30-34
AGTAG 10937755 4.1566315 28.351406 80-89
ATGCA 9345110 3.973622 95.25178 25-29
GACCA 9851140 3.9661233 106.14647 45-49
CCCAG 12127025 3.9573598 86.18809 60-69
TGAGG 13707050 3.7734632 68.56882 35-39
CCACT 9638290 3.7167428 26.983494 240-247
TACAC 7740165 3.6824863 27.53677 110-119
CAGGG 14055645 3.6637387 35.536446 50-59
ATGGT 11238635 3.656124 25.284485 190-199
AGGAG 11367525 3.6556787 93.845726 35-39
ACGGT 11771735 3.6259804 95.71967 40-44
GACGG 13899050 3.6229208 106.97785 40-44
CTGAG 11714725 3.60842 104.345314 35-39
TGTCC 12047835 3.5544853 20.576193 120-129
CCCTT 10708985 3.5351253 35.08961 20-24
AGATA 6715925 3.5231924 18.84012 150-159
TGCAC 10212670 3.5197594 115.37542 25-29
GGGTT 14765630 3.479703 42.5933 50-59
GAGGA 10730305 3.450756 122.60385 35-39
GCCCC 12973165 3.4313803 73.039604 60-69
TGGTG 14517360 3.4211948 17.784845 190-199
CCTGA 9765965 3.3658042 84.577286 30-34
GGATG 12053825 3.3183413 69.90106 20-24
TCTTG 10574945 3.2950978 17.882185 170-179
CCAGG 11293650 3.293797 40.39863 50-59
CCGTG 13170675 3.2882478 23.577784 120-129
GCACT 9467725 3.2630172 89.52188 25-29
CTTGG 12350675 3.2566392 20.048992 20-24
CTTCA 7994490 3.2559347 16.44159 200-209
AGGCT 10561535 3.2532094 24.585396 30-34
CTCTC 9827780 3.2442322 13.77689 100-109
CAGAT 7530310 3.2019534 17.658766 150-159
TCAGG 10361595 3.191623 20.62924 140-149
AAAAA 3769535 3.1889045 19.125818 130-139
CCTGG 12748750 3.1829083 29.853508 50-59
TCGCA 9079185 3.129108 24.217443 100-109
TGTCT 10005445 3.1176443 19.456139 180-189
TCTCA 7506230 3.05708 21.53094 130-139
CGCAC 9343745 3.0491045 24.751091 100-109
GGTTG 12917810 3.0442414 74.070526 15-19
TTCCC 9190365 3.033816 50.348175 50-59
CGGAT 9779765 3.0124052 79.415405 20-24
ACAGA 5990940 2.9757953 14.364544 140-149
GGTTC 11169685 2.9452345 48.12124 50-59
TGGCC 11671160 2.9138725 43.52857 60-69
GATGC 9458335 2.9133966 96.08071 25-29
AGGGT 10559050 2.9068394 50.074993 50-59
ACCAC 6379775 2.8739147 19.150145 230-239
GACTC 8260100 2.8468137 19.803047 190-199
GCCCT 10145100 2.8340116 18.788866 20-24
GTTGG 12008600 2.829975 53.22894 15-19
TCTCT 8030170 2.7996495 14.667109 180-189
CGGTG 12367950 2.759723 68.146866 40-44
TCAAA 4696430 2.7566853 56.815308 70-79
AGTCA 6482695 2.756498 31.595757 70-79
GCCGT 11034955 2.755035 22.250547 120-129
CCAGT 7955990 2.7420032 37.051785 60-69
GATGG 9849780 2.7115815 19.179674 15-19
TTCTT 7360875 2.710384 20.137058 170-179
CGGCC 11443705 2.7052088 12.836796 120-129
GTTCC 9062965 2.673856 54.49882 50-59
TCACA 5464290 2.5997086 11.560961 140-149
AGATG 6782435 2.5775018 16.763412 180-189
TCCAT 6274800 2.5555525 17.192997 70-79
AGGGA 7864010 2.528985 9.182991 150-159
GCGGA 9591370 2.5000827 86.99532 20-24
GGCTG 11175270 2.4935942 12.882788 30-34
TCCTC 7460930 2.4629152 16.67609 130-139
TGGAG 8876195 2.44356 16.7835 25-29
TGTTC 7830875 2.4400597 12.869108 140-149
GGCGG 12885075 2.4330077 42.50021 20-24
ACTCT 5942955 2.4204013 13.562279 200-209
CACGG 8246645 2.405137 9.717477 120-129
TACCA 4988195 2.3732 16.00528 230-239
ACAGC 5886495 2.3699353 10.877916 120-129
TTGGG 10046050 2.3674757 79.917465 15-19
CTGGC 9415265 2.350656 35.014046 60-69
GAGAT 6173280 2.346007 16.27735 180-189
GTTCT 7514085 2.3413496 17.68493 160-169
CAGCT 6785525 2.3386066 12.267605 150-159
AATAA 3202335 2.3190734 9.650797 110-119
TGTAG 7122660 2.3171256 8.174188 210-219
CTGGA 7409535 2.2823167 14.130512 180-189
TCAGC 6480120 2.23335 14.770876 140-149
CATTT 5138035 2.2100618 17.152521 150-159
TCATT 5126320 2.2050228 15.978286 150-159
CTACT 5386970 2.193964 7.4525943 180-189
ACTAC 4605895 2.1913157 12.107776 230-239
CAGAG 6067025 2.1830654 9.435941 140-149
TTCAT 5047000 2.1709042 15.809299 140-149
TTCAC 5330295 2.1708817 18.093096 200-209
CACTA 4511660 2.1464822 14.518445 240-247
GTGAA 5641145 2.143782 17.254759 190-199
TCTGC 7240935 2.1363008 11.8614645 210-219
TTGTC 6852400 2.135172 13.168218 170-179
GATAC 4967160 2.1120796 14.3007145 150-159
TGGGG 10579020 2.1097178 52.920364 15-19
CTCGC 7502280 2.0957458 16.780798 100-109
ATTTG 5414915 2.0816622 15.055735 150-159
TAGTC 5707680 2.0775704 26.34821 70-79
TCTCG 7026570 2.0730565 16.901642 100-109
GAACT 4860045 2.0665336 10.943303 160-169
TTGGA 6342425 2.0633016 15.757856 170-179
ATCTC 5059470 2.0605822 8.763853 100-109
ATGTA 4571820 2.0531168 7.684569 70-79
ACGGC 7001110 2.0418763 14.385286 120-129
CTCTG 6920645 2.0418053 14.1944 180-189
GCTGT 7742035 2.041428 9.837449 140-149
AGCTC 5901150 2.0338101 12.642213 140-149
ATATA 3268130 2.0260077 10.1619005 110-119
GTCTC 6859560 2.0237832 11.570236 180-189
ACACA 3640400 2.0232337 13.584762 110-119
GTCCT 6834665 2.0164385 14.086885 130-139
GATAT 4452305 1.999445 8.224294 180-189
ACACG 4963125 1.9981815 13.315747 120-129
GAATC 4689170 1.9938757 13.99422 190-199
GTCAA 4681335 1.9905441 40.339367 70-79
TACAG 4679055 1.9895749 13.271678 160-169
CAGGC 6777470 1.9766515 14.987778 140-149
GTTCA 5420545 1.9730545 14.856388 140-149
AACCA 3496140 1.9430578 14.963186 70-79
TGAAT 4323810 1.9417403 13.385698 190-199
AATCG 4563055 1.9402506 13.200767 190-199
AATAT 3102125 1.9230963 12.672615 110-119
TTGCA 5280725 1.9221606 13.847072 150-159
CCTTC 5807700 1.9171703 13.663788 200-209
TTTGC 6145665 1.914957 11.816207 150-159
TGGAA 5038435 1.9147364 8.998357 170-179
TACTA 3764810 1.891718 9.696556 230-239
GAGTC 6124020 1.8863469 11.878391 210-219
ATATG 4171705 1.8734329 8.482097 180-189
GGGGC 9916475 1.8724658 48.21518 15-19
CAGCC 5708755 1.8629136 10.002151 120-129
TCGGC 7393540 1.8459034 7.727417 130-139
CATAG 4339360 1.8451337 8.244508 210-219
GGGCG 9659685 1.8239776 59.404476 20-24
GACGT 5822520 1.7934777 9.856452 170-179
GTTGT 6415195 1.7865338 7.106475 210-219
CTCCA 4603295 1.7751348 8.010791 150-159
AGTAT 3951745 1.7746531 11.418881 220-229
CCAGA 4389705 1.7673194 26.109158 60-69
CTGTT 5654640 1.7619563 12.497302 140-149
AGAAC 3533910 1.7553495 11.343161 160-169
CGTAG 5683040 1.7505144 9.560123 210-219
GTCAC 5064025 1.7452978 8.82346 130-139
ATTGT 4448755 1.71024 7.55889 50-59
CACCA 3794080 1.7091296 13.124871 230-239
TGACT 4640555 1.6891416 11.867065 190-199
GGTCA 5483610 1.6890851 7.7574315 130-139
GCAGA 4690710 1.6878332 14.056639 150-159
TCCCA 4376435 1.6876525 10.88498 240-247
AGTCT 4624310 1.6832285 12.614113 210-219
TGCAG 5450730 1.678957 13.17739 150-159
CCTTG 5676910 1.6748649 22.771404 20-24
TCAGA 3895995 1.656611 10.32242 130-139
ATAGT 3674385 1.6500959 7.208052 220-229
TTCAG 4532030 1.649639 9.436446 150-159
TGGTT 5916165 1.6475614 17.83983 10-14
AACTG 3860100 1.641348 10.765299 160-169
GTCTG 6210685 1.63764 8.773149 210-219
CAAAA 2385420 1.6356492 20.55851 70-79
GCTCC 5824140 1.626961 6.473665 150-159
TGTGC 6167520 1.6262583 8.188704 220-229
GAGAA 3659990 1.6247975 10.362602 160-169
TGCTC 5499370 1.6224849 5.455319 150-159
CTGTG 6136825 1.6181645 5.3890305 210-219
TAGTT 4178200 1.6062301 9.925311 220-229
GTGCT 6049815 1.5952215 8.193196 220-229
TTGTA 4143640 1.5929443 9.109723 210-219
AATAG 3035925 1.5926545 9.235163 240-247
ACTGA 3734250 1.5878357 8.903219 180-189
TTGGT 5693825 1.5856433 23.847015 25-29
CTGGT 6012355 1.5853442 7.1227336 160-169
ATAGA 3015800 1.5820969 10.075474 240-247
GTCCC 5650645 1.5784954 19.962387 50-59
TCTGG 5955295 1.5702986 11.622569 180-189
CAAAG 3150410 1.5648588 28.58727 70-79
GGCCG 7378205 1.5588202 13.228744 120-129
GCTCA 4496375 1.5496593 11.367108 140-149
GAACC 3829810 1.5419027 12.531836 70-79
GGACG 5874755 1.5313113 7.3010607 170-179
CCCAC 4162150 1.5196998 10.187649 240-247
ACCAT 3177725 1.5118449 11.4273 45-49
CCACC 4115120 1.5025281 9.526831 230-239
GCTCT 4930380 1.4546154 9.979341 130-139
CATGT 3969040 1.4447131 7.638874 70-79
CCTCG 5166395 1.4432212 9.335026 130-139
GCTGA 4650165 1.4323637 21.019424 30-34
CACTG 4155410 1.432147 7.3527803 230-239
CGACT 4151620 1.4308408 8.794063 190-199
CAGAC 3541665 1.4258939 15.23959 60-69
TATAC 2805680 1.4097804 6.552562 110-119
TGGAC 4552375 1.402242 10.441993 170-179
CTCTT 4014065 1.3994691 8.255696 200-209
TCGAC 4052400 1.3966451 9.415821 190-199
TGATA 3103905 1.3939042 7.72804 180-189
GGGAG 5968820 1.3905079 6.6812243 150-159
GAGGG 5965135 1.3896495 8.407937 200-209
CTGCT 4700900 1.3869116 5.7460465 90-99
AGATC 3218200 1.3684065 7.090257 130-139
CCTCA 3542420 1.3660374 7.37735 130-139
AATTG 3040840 1.3655831 9.088849 170-179
GCGTA 4415675 1.3601352 10.041996 210-219
CTTGA 3711040 1.3508024 8.434591 200-209
CCATC 3482815 1.3430523 10.960227 240-247
ACTTC 3278430 1.3352139 6.615703 220-229
GTAGG 4834155 1.330812 5.165654 160-169
GTGGA 4812940 1.3249717 21.236216 25-29
CTGAT 3633795 1.3226854 7.359471 180-189
CCATG 3801840 1.3102905 7.7336307 70-79
CTCGA 3799105 1.3093476 8.4878645 190-199
ACGGA 3631035 1.306536 9.127942 200-209
TCCAC 3382320 1.3042991 9.693052 240-247
TATGG 3958535 1.2877805 6.1720095 180-189
GAATT 2831230 1.2714512 10.064591 170-179
ACGTG 4127445 1.2713534 9.524465 170-179
GTGTT 4535070 1.2629476 7.4284606 160-169
ACCGT 3661600 1.2619572 18.232674 45-49
ATCGG 4085530 1.2584425 7.812709 190-199
GGAGG 5385100 1.2545234 15.986288 30-34
GGAAT 3299580 1.2539263 8.3679695 170-179
ACTAT 2477815 1.2450368 6.145281 230-239
AGCCG 4259660 1.2423314 8.897546 120-129
GGAGT 4494990 1.2374421 6.786032 210-219
ACTCG 3582595 1.2347285 8.723121 190-199
GGCTC 4934910 1.2320712 8.281489 130-139
GATCT 3375590 1.2286999 5.6576405 130-139
AAGTA 2342030 1.2286353 15.70734 70-79
TCACG 3557535 1.2260916 9.428596 200-209
ATAAC 2084560 1.2235838 7.4600945 80-89
GTGGT 5191175 1.2233644 10.5028715 50-59
GTCCA 3547055 1.2224796 16.073284 70-79
AAAGT 2327505 1.2210155 20.998981 70-79
ACTGG 3935440 1.2122111 7.277768 160-169
ATAAA 1669560 1.2090653 5.279552 7
TTGAG 3687640 1.1996536 8.106872 200-209
TGGTC 4549010 1.1994877 11.879513 50-59
TGCCC 4259590 1.1899072 6.7673764 60-69
AGCTT 3256630 1.185399 6.231255 150-159
TTGGC 4490575 1.1840796 8.106967 60-69
GCATA 2774305 1.1796587 7.9930053 210-219
AGAGC 3249060 1.1690919 7.093918 140-149
TTCCA 2850430 1.1609013 7.3311467 230-239
ACTGT 3171980 1.1545868 5.601703 230-239
ATACC 2426430 1.1544062 7.212747 70-79
TCTGT 3669050 1.1432569 6.5333786 210-219
CGGCT 4560455 1.1385831 7.6591907 130-139
TATCA 2249255 1.1301914 6.7910986 70-79
AAAAG 1843765 1.1299055 13.709379 70-79
TCTAC 2739075 1.1155496 7.2489157 180-189
CCATA 2328680 1.1079004 10.068168 70-79
GAAGA 2493180 1.1068097 17.98277 35-39
ATCCA 2324245 1.1057904 15.583198 240-247
CAATA 1877080 1.1017983 6.529742 100-109
GTCTA 3014345 1.0972084 7.3540316 180-189
ATATC 2175295 1.0930285 7.2933064 70-79
AGGTT 3359420 1.092878 22.921297 10-14
AGTTC 2995240 1.0902542 5.264763 160-169
GCTTC 3690140 1.088706 5.670935 150-159
CTGAA 2524105 1.0732714 14.326287 35-39
TACTG 2947375 1.0728315 6.4937487 180-189
GAGCT 3478285 1.0713962 6.428356 140-149
CTCGG 4250975 1.0613168 8.120465 130-139
CTTCT 3042225 1.0606455 5.5189295 210-219
CTGCC 3796845 1.0606405 5.8470025 200-209
TATGT 2758705 1.0605321 7.2426486 220-229
CTACC 2711820 1.0457392 8.367033 230-239
CTGCG 4183210 1.0443983 6.683171 210-219
AACTT 2076955 1.0436152 5.9963174 200-209
AGAGA 2350620 1.0435224 12.614602 35-39
GTCCG 4156915 1.0378333 5.028795 130-139
GGGGT 5204090 1.0378242 7.926233 10-14
ATGGG 3769495 1.0377179 30.393217 15-19
TAGAT 2310545 1.0376215 7.778937 240-247
AAAAT 1432025 1.0370468 5.365335 8
CATGG 3351500 1.0323434 5.298869 190-199
GCGGT 4620685 1.0310367 5.933295 130-139
TGCGT 3776465 0.99578226 8.080664 210-219
TATAA 1564625 0.9699559 6.296144 240-247
TCCCC 3090310 0.9659088 5.025142 80-89
AAGAG 2173645 0.96495694 13.31969 35-39
CAGCG 3300865 0.96269846 6.1524377 160-169
CGAAC 2379410 0.9579636 13.041214 70-79
ACCCA 2125380 0.9574258 12.700483 240-247
AGCTG 3088900 0.95145625 5.1135683 90-99
TAGGC 3084490 0.95009786 5.817095 160-169
TGTAA 2110830 0.9479334 6.9008613 230-239
ACAAT 1611860 0.9461208 7.5324435 100-109
TTCTG 3016775 0.9400114 5.454961 210-219
CGTGG 4207910 0.9389321 7.2763515 50-59
CGGAG 3597825 0.9378076 6.3675904 210-219
CCATT 2278705 0.9280536 5.8215675 50-59
CCCAA 2053770 0.9251673 9.81817 240-247
ACTAA 1576005 0.925075 11.813629 240-247
TGCAT 2498280 0.90936285 6.1559443 210-219
GAGTT 2781450 0.9048542 5.0364747 160-169
ACGTC 2595675 0.89459 11.044766 70-79
TCGAA 2083935 0.8861073 16.584814 70-79
TGAAG 2325490 0.88374674 9.405074 35-39
TGAAC 2076795 0.88307124 6.189638 190-199
GGTGG 4401870 0.87784165 21.248285 25-29
CCAAT 1830420 0.8708466 10.492407 240-247
CATCA 1822500 0.8670786 5.579486 230-239
GTATG 2649020 0.8617723 5.142348 220-229
CCAGC 2612125 0.8524036 5.6859484 80-89
CGGTC 3411465 0.8517212 5.6829724 130-139
GAGGC 3260155 0.84979075 13.588724 30-34
ATCAA 1426360 0.8372372 12.48042 70-79
CTTCC 2509915 0.8285439 5.2071004 230-239
AGCGT 2685230 0.8271161 6.319454 160-169
AAAGA 1348950 0.82667035 5.1515937 8
GGGCC 3798080 0.80243427 16.259594 15-19
TAACC 1655255 0.7875095 6.032918 80-89
GACCG 2682690 0.7824075 16.888071 45-49
CGTCC 2798065 0.78163344 9.130849 70-79
AACCG 1923615 0.774458 5.472246 190-199
TGAGA 2034785 0.77327126 5.621929 240-247
TGGGC 3379990 0.75419414 17.637068 15-19
AAAGG 1683745 0.74747324 8.543433 10-14
GGTCC 2984085 0.7450197 12.119844 50-59
ATGAT 1647905 0.74004257 6.85938 240-247
AATCC 1552455 0.738601 9.48641 240-247
AGCCA 1834295 0.73849726 5.904296 80-89
CTAAT 1460045 0.73363423 9.161388 240-247
GTCGA 2348845 0.72350127 11.905491 70-79
GTAGC 2302845 0.7093321 5.4859586 80-89
CATAC 1482620 0.70537615 8.595162 70-79
AAGGT 1816835 0.6904446 9.855378 10-14
GGAAG 2136315 0.68701684 5.8500466 30-34
AAAGC 1355670 0.67338294 9.493516 70-79
CATTG 1834125 0.66761345 5.299891 50-59
CAATC 1388610 0.6606496 9.252116 240-247
GAAAA 1075500 0.6590934 5.761362 8
TAGGT 2008305 0.65333664 5.3700733 10-14
AGGAT 1704720 0.64783794 5.778605 10-14
TATGA 1416565 0.6361522 5.5067353 240-247
GAGGT 2295100 0.6318264 6.7380023 10-14
AGAAG 1398615 0.6208941 5.311811 9
ATAGG 1623630 0.6170217 5.1052136 10-14
CCGTA 1754600 0.6047165 5.1204896 80-89
CGATG 1951080 0.60098004 13.168055 15-19
TAAAA 815005 0.590212 5.6490874 8
AATGG 1552690 0.5900626 6.6668844 10-14
GGGTC 2629395 0.58671016 5.676049 70-79
AAGAA 952365 0.5836331 5.195745 9
CATCC 1510735 0.5825736 7.430047 240-247
AAATG 1083350 0.5683284 5.6173773 9
GAAGG 1701465 0.54717356 5.5146136 10-14
AAGGG 1626645 0.52311224 6.3449516 10-14
AAGCA 1047550 0.52033484 6.264731 70-79
TGATC 1416410 0.515567 5.495481 240-247
GGTCG 2225740 0.49664056 5.794336 70-79
GATCC 1434575 0.49442104 5.569245 240-247
ATAAG 894490 0.4692519 5.50722 9
ACGAT 1068045 0.45414203 6.1157556 10-14
CGGTT 1678865 0.44268492 8.136544 10-14
TAAGG 1160370 0.4409708 5.911537 10-14
TAAAG 777205 0.40772384 5.5448027 9
AAGCT 929765 0.39534417 7.6700716 30-34
TGGGT 1671555 0.39392254 5.0555487 10-14
Produced by FastQC (version 0.10.1)