FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_9_SL248355.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_9_SL248355.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12195956
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13986020 8.524275 59.902134 110-119
ATACA 12786800 7.3843637 36.918457 110-119
CACAG 18621720 7.3764586 41.59211 110-119
TCCCT 21816310 7.0412188 106.46561 30-34
CAGTA 15721220 6.572437 38.719025 110-119
AGTAA 12128120 6.267091 46.83051 110-119
GGAGA 19251150 6.1055856 93.67528 35-39
CCCTG 21758610 5.953973 69.75005 30-34
GTAAT 13246360 5.84452 39.06659 110-119
GGTGA 21560625 5.8386416 101.81243 45-49
AATAC 9294215 5.367399 38.90117 110-119
GTAGT 16440555 5.2511907 28.71624 80-89
AGACG 14679735 5.203165 100.81548 40-44
ACAGT 12273080 5.1309023 35.129654 110-119
GTGAC 16505805 4.9953504 84.24231 45-49
GAGAC 13364970 4.737153 139.97221 40-44
CCCCA 13202835 4.728701 101.04371 60-69
ACTCC 12365910 4.6742597 120.51344 30-34
CTCAG 13735240 4.6456256 34.5044 140-149
TAGTA 10230645 4.513935 36.393932 80-89
ACCAG 11378850 4.507404 92.56749 45-49
GGCCC 19052165 4.4200706 59.277718 60-69
GCACA 11002865 4.3584685 33.78109 110-119
GTGTC 16680560 4.3104205 23.694223 120-129
CACTC 11378445 4.301002 98.133415 25-29
CGTGT 16532750 4.272225 23.14443 120-129
TGACC 12622800 4.2693686 144.00298 45-49
AGTAG 11193345 4.1871777 29.608828 80-89
CTCCC 13595010 4.157517 69.528885 30-34
ATGCA 9502780 3.972747 95.333626 25-29
CCCAG 12389715 3.97061 86.4453 60-69
GACCA 9980870 3.953635 105.18154 45-49
TGAGG 13874040 3.7571056 68.92812 35-39
AGGAG 11625240 3.6869953 93.9065 35-39
CCACT 9714990 3.6722236 26.821657 240-247
ATGGT 11465905 3.6622639 25.366138 190-199
TACAC 7830255 3.658431 27.007784 110-119
ACGGT 12053385 3.6478608 95.89455 40-44
GACGG 14198790 3.6432564 107.666145 40-44
CAGGG 14168405 3.63546 35.490475 50-59
CTGAG 11938950 3.6132276 104.73184 35-39
CCCTT 11181195 3.6087332 35.702576 20-24
TGTCC 12387750 3.577505 20.609543 120-129
AGATA 6888085 3.5593522 19.023634 150-159
TGCAC 10434440 3.529207 115.213844 25-29
GAGGA 10954285 3.474199 123.267815 35-39
GCCCC 13261175 3.4383147 73.17223 60-69
GGGTT 14797365 3.4214852 42.199455 50-59
TGGTG 14795025 3.420944 17.801956 190-199
CCTGA 9926325 3.357349 83.911835 30-34
CAGAT 7982025 3.3369772 17.718561 150-159
GGATG 12262875 3.3208003 69.718414 20-24
CCGTG 13499060 3.305221 23.266123 120-129
AGGCT 10900175 3.298851 24.890923 30-34
CTTGG 12721385 3.2873309 20.393337 20-24
CCAGG 11433795 3.2787442 40.216484 50-59
TCTTG 10748585 3.2760546 17.766014 170-179
CTCTC 10086430 3.2553976 13.56866 100-109
GCACT 9614990 3.2520466 89.07356 25-29
CTTCA 8137010 3.2461128 16.601412 200-209
TCAGG 10580090 3.2019796 20.644686 140-149
CCTGG 12965910 3.1746802 29.439137 50-59
TCGCA 9232965 3.1228356 23.933168 100-109
TCTCA 7777030 3.1025054 21.691723 130-139
TGTCT 10153855 3.0947876 19.412062 180-189
CGCAC 9495580 3.0431082 24.475674 100-109
GGTTG 13058765 3.0194814 73.96523 15-19
CGGAT 9947430 3.0105104 79.101006 20-24
TTCCC 9291095 2.9987032 49.650337 50-59
ACAGA 6113455 2.9932785 13.893872 140-149
TGGCC 11936950 2.9227414 43.544422 60-69
GATGC 9607700 2.9076939 96.100204 25-29
GGTTC 11242170 2.9050872 47.628677 50-59
AGGGT 10624745 2.877193 49.644844 50-59
ACCAC 6494315 2.8750157 19.135334 230-239
GCCCT 10494130 2.8715882 19.252432 20-24
AAAAA 3375450 2.821859 14.148163 130-139
GTTGG 12195335 2.819837 53.15345 15-19
TCTCT 8257755 2.8128111 14.957115 180-189
GACTC 8300015 2.8072872 19.092607 190-199
CGGTG 12663095 2.7743297 68.187675 40-44
TCAAA 4801740 2.7729995 57.036488 70-79
GATGG 10193240 2.760341 19.812134 15-19
GCCGT 11204500 2.743402 22.014868 120-129
AGTCA 6519685 2.7256298 31.366356 70-79
CGGCC 11675925 2.7087953 12.606424 120-129
CCAGT 7988255 2.7018416 36.167988 60-69
TTCTT 7512260 2.700606 20.10615 170-179
GTTCC 9174125 2.6494303 53.918552 50-59
AGATG 7019525 2.6258454 16.937021 180-189
TCACA 5573515 2.6040425 11.2392235 140-149
TCCAT 6466825 2.5798228 17.93314 70-79
GGCTG 11556790 2.5319517 13.064032 30-34
GCGGA 9768730 2.5065508 87.14102 20-24
TGGAG 9219715 2.496709 17.034512 25-29
TCCTC 7726205 2.4936345 16.835346 130-139
AGGGA 7834915 2.4848773 8.884935 150-159
TGTTC 8099160 2.4685383 12.765463 140-149
GGCGG 13102215 2.4337282 42.570385 20-24
ACAGC 6083010 2.4096096 11.100616 120-129
ACTCT 6019190 2.401247 13.244814 200-209
GAGAT 6380305 2.3867278 16.418705 180-189
TTGGG 10216990 2.362399 79.76079 15-19
TACCA 5043430 2.3563778 15.999394 230-239
GTTCT 7672045 2.3383582 17.391846 160-169
AATAA 3273850 2.3369112 9.833283 110-119
CTGGC 9518185 2.3305106 34.78918 60-69
CTGGA 7667455 2.320494 14.413221 180-189
CAGCT 6846645 2.3157184 12.054327 150-159
CACGG 8044310 2.3067787 9.498318 120-129
TGTAG 7159125 2.286658 7.8082476 210-219
CATTT 5339100 2.2479098 17.495564 150-159
TCATT 5315520 2.2379818 16.235453 150-159
TTCAT 5230320 2.2021105 15.842378 140-149
CAGAG 6198375 2.1969857 9.182643 140-149
TTCAC 5506080 2.196551 18.183086 200-209
GTGAA 5866705 2.1946018 17.72404 190-199
TCAGC 6485390 2.1935322 14.344714 140-149
ACTAC 4664030 2.1791158 12.063057 230-239
TTGTC 7102160 2.1646633 13.281595 170-179
CTACT 5413115 2.1594641 7.2628345 230-239
CACTA 4566420 2.1335106 14.681539 240-247
GATAC 5102785 2.1332784 14.375917 150-159
ATTTG 5601840 2.1103904 15.452206 150-159
TCTGC 7298755 2.1078348 11.999633 210-219
TGGGG 10745675 2.1065567 52.720448 15-19
CTCGC 7614875 2.083716 16.416046 100-109
GCTGT 7984590 2.0632966 10.048185 140-149
CTCTG 7134320 2.0603468 14.450832 180-189
TAGTC 5752775 2.0535128 26.022848 70-79
TCTCG 7104855 2.0518377 16.298376 100-109
GAATC 4907915 2.0518103 14.272815 190-199
TTGGA 6408135 2.0467885 15.334055 170-179
ATGTA 4638960 2.0467882 7.85542 70-79
GTCCT 7080515 2.0448086 14.188153 130-139
GTCTC 7068140 2.041235 11.778676 180-189
ACACA 3725930 2.0387962 13.519584 110-119
ATCTC 5108670 2.0380116 8.344195 100-109
GAACT 4871475 2.0365763 10.490223 160-169
ATATA 3314625 2.0202155 10.334516 110-119
TACAG 4830565 2.0194736 13.43189 160-169
AGCTC 5941600 2.0096083 12.194236 140-149
CAGGC 7007755 2.0095372 15.289864 140-149
TGAAT 4542600 2.0042727 13.748669 190-199
GTTCA 5594050 1.9968545 14.829229 140-149
AATCG 4725240 1.975441 13.263088 190-199
GATAT 4467985 1.9713513 7.9659877 180-189
GTCAA 4715340 1.9713024 40.01585 70-79
TTGCA 5498620 1.9627898 14.173159 150-159
AATAT 3220200 1.9626646 12.96044 110-119
ACACG 4939525 1.956651 13.113971 120-129
TTTGC 6405875 1.9524428 12.108588 150-159
CCTTC 6035485 1.9479543 13.849007 200-209
TGGAA 5174045 1.9354934 9.044461 170-179
AACCA 3536440 1.9351089 14.683883 70-79
ACGGC 6736580 1.9317753 14.222867 120-129
GAGTC 6335290 1.917325 12.123937 210-219
TCGGC 7725890 1.8916705 7.8270802 130-139
TACTA 3824850 1.8860164 9.741417 230-239
CAGCC 5871320 1.8816189 10.145636 120-129
GGGGC 10112515 1.8783935 48.133644 15-19
ATATG 4246995 1.8738465 8.098815 180-189
CATAG 4472430 1.8697511 8.277615 210-219
GGGCG 9821405 1.8243198 59.514927 20-24
CCAGA 4590690 1.81847 27.194311 60-69
AGTAT 4085610 1.8026408 11.561879 220-229
GACGT 5905695 1.7873116 9.38151 170-179
CTGTT 5852195 1.783687 12.506746 140-149
GTTGT 6512905 1.7762163 6.8126283 210-219
CGTAG 5821980 1.7619756 9.594823 210-219
TCAGA 4213695 1.7615836 10.182411 130-139
CTCCA 4658460 1.7608771 7.988641 150-159
ATTGT 4574510 1.7233627 7.6874356 50-59
GCAGA 4859525 1.7224364 14.218751 150-159
AGAAC 3503640 1.7154571 10.998966 160-169
CCTTG 5932325 1.7132185 23.188232 20-24
TGCAG 5658685 1.7125559 13.382448 150-159
CACCA 3860615 1.7090838 13.170972 230-239
GTCAC 5044710 1.7062559 8.390549 130-139
AGTCT 4762655 1.7000792 12.788505 210-219
ATAGT 3786280 1.6705713 7.2739286 220-229
GGTCA 5504035 1.6657523 7.4029913 130-139
TGACT 4641435 1.6568085 11.491651 190-199
CAAAA 2428200 1.6423128 21.017347 70-79
TCCCA 4334410 1.6383877 10.456642 240-247
TGGTT 5997465 1.6356441 18.089901 10-14
AACTG 3876620 1.6206657 10.376131 160-169
TTCAG 4528955 1.6166576 9.07975 150-159
GTCTG 6255975 1.6166055 8.942082 210-219
TGTGC 6243695 1.6134323 8.057412 220-229
CTGTG 6220780 1.6075107 5.359722 210-219
GTCCC 5874405 1.607458 20.646826 50-59
GCTCC 5855965 1.602412 6.412153 150-159
GAGAA 3635580 1.5927784 10.093446 160-169
TGCTC 5504180 1.5895729 5.354674 150-159
TCTGG 6149435 1.5890744 11.828112 180-189
TTGGT 5819740 1.5871745 24.431166 25-29
AATAG 3060080 1.5812669 9.126059 240-247
TAGTT 4189240 1.5782193 9.666639 220-229
GTGCT 6069765 1.568487 7.867393 220-229
ACTGA 3745035 1.5656552 8.48013 180-189
CTGGT 6042315 1.5613936 6.849499 160-169
TTGTA 4138730 1.5591905 8.686749 210-219
CAAAG 3176990 1.5555223 28.584654 70-79
GAACC 3900805 1.5451918 12.222464 70-79
GGCCG 7443065 1.5451049 13.014921 120-129
ATAGA 2984765 1.542349 9.565683 240-247
ACCAT 3273940 1.5296415 11.735802 45-49
GCTCA 4518480 1.5282707 10.954083 140-149
GGACG 5913635 1.517375 6.9874353 170-179
CCACC 4222470 1.5123116 9.512858 230-239
CCCAC 4184265 1.498628 10.161045 240-247
CAGAC 3714300 1.4713131 16.085423 60-69
GCTCT 5072895 1.4650203 9.900363 130-139
CCTCG 5344180 1.4623685 9.515369 130-139
AGATC 3474870 1.4527094 7.0640664 130-139
CATGT 4060425 1.4494109 7.876242 70-79
GCTGA 4785635 1.4483341 21.409586 30-34
CGACT 4185250 1.4155635 8.4260235 190-199
CACTG 4148810 1.4032384 7.043764 230-239
TATAC 2825930 1.3934534 6.604729 110-119
TCGAC 4094010 1.3847036 9.081427 190-199
TGATA 3126595 1.3795071 7.4262886 180-189
CTCTT 4047920 1.3788292 7.8697643 200-209
TGGAC 4552895 1.3778974 10.080033 170-179
AATTG 3117185 1.3753549 8.978695 170-179
CCTCA 3629805 1.3720503 7.399338 130-139
CTGCT 4742100 1.3694887 5.705865 90-99
GCGTA 4491220 1.3592318 10.004745 210-219
GGGAG 5905375 1.3558359 6.406612 150-159
GAGGG 5898490 1.3542551 8.02119 200-209
CCATC 3557030 1.3445417 10.743798 240-247
GTGGA 4942310 1.3383831 21.715715 25-29
ACTTC 3338040 1.3316507 6.6919374 220-229
GTAGG 4893800 1.3252466 5.190174 160-169
CTTGA 3686690 1.3160024 8.026657 200-209
CCATG 3888520 1.3152014 8.032942 70-79
ACGGA 3704430 1.3130183 9.128375 200-209
GATCT 3678190 1.3129681 5.617243 130-139
CTGAT 3669450 1.3098482 7.1622257 180-189
TCCAC 3457740 1.3070104 9.361704 240-247
ACCGT 3861505 1.3060642 19.135263 45-49
CTCGA 3831215 1.2958193 8.106988 190-199
ATCGG 4246965 1.2853101 7.931574 190-199
TATGG 4017770 1.2832944 5.9296336 190-199
GAATT 2903235 1.2809567 9.963555 170-179
GTGTT 4670730 1.2738135 7.428789 160-169
AGCCG 4404680 1.2630819 9.048437 120-129
GGAGT 4655845 1.2608081 6.8619037 210-219
GTCCA 3720025 1.2582121 16.74269 70-79
GGAAT 3358000 1.2561518 8.378627 170-179
GGAGG 5456775 1.2528403 16.277136 30-34
GGCTC 5076010 1.242852 8.234314 130-139
GTGGT 5366480 1.2408514 10.963402 50-59
ACGTG 4098045 1.2402406 9.036376 170-179
ACTAT 2506125 1.235759 6.001065 230-239
ATAAC 2134235 1.2325186 7.444238 80-89
TCACG 3624755 1.2259891 9.344839 200-209
AAGTA 2370635 1.225003 15.536284 70-79
TTGGC 4725665 1.2211584 8.344704 60-69
AAAGT 2362405 1.22075 20.98961 70-79
ATAAA 1701615 1.2146322 5.43255 7
TGGTC 4686895 1.2111397 12.399133 50-59
ACTCG 3563145 1.2051508 8.315975 190-199
GCATA 2872185 1.20075 7.9474325 210-219
TGCCC 4352215 1.1909297 6.9890876 60-69
TTCCA 2960915 1.1812036 7.492015 230-239
ACTGG 3898935 1.1799816 6.9377565 160-169
TTGAG 3682715 1.1762764 7.7658596 200-209
ATACC 2497245 1.1667563 7.6373754 70-79
AGCTT 3214440 1.1474278 5.935189 150-159
AGAGC 3222755 1.142291 6.792793 140-149
AAAAG 1881585 1.1387187 14.126184 70-79
TCTGT 3732135 1.1375152 6.5411816 210-219
ACTGT 3180185 1.1352001 5.3928413 230-239
TATCA 2301705 1.1349604 6.8623457 70-79
CCATA 2397705 1.1202493 10.516153 70-79
GAAGA 2555620 1.1196388 18.274126 35-39
CAATA 1932985 1.1162968 6.545563 100-109
AGTTC 3124600 1.1153585 5.4102206 160-169
ATATC 2232675 1.100922 7.3623643 70-79
AGGTT 3444205 1.1000953 23.109852 10-14
TCTAC 2719365 1.0848415 7.013042 180-189
ATCCA 2321655 1.0847174 14.914363 240-247
CGGCT 4418215 1.0817921 7.723163 130-139
GTCTA 3023660 1.079327 7.093598 180-189
CTGAA 2577385 1.0775055 14.600849 35-39
CTCGG 4382730 1.0731037 8.22869 130-139
TATGT 2843150 1.0711045 7.330319 220-229
GCTTC 3686615 1.0646714 5.3278904 150-159
ATGGG 3893090 1.0542531 31.077578 15-19
CTGCC 3851465 1.0539055 5.8080935 200-209
TACTG 2951390 1.0535294 6.240645 180-189
AGAGA 2402955 1.052755 13.015915 35-39
GAGCT 3459390 1.0469568 6.0956497 140-149
CTTCT 3047705 1.0381294 5.540735 210-219
CTACC 2745850 1.037919 8.305435 230-239
CTGCG 4230055 1.0357214 6.694164 210-219
AACTT 2097545 1.0342898 5.918003 200-209
TAGAT 2316265 1.0219756 7.410823 240-247
CATGG 3371560 1.0203757 5.3071856 190-199
AAAAT 1427995 1.0193189 5.2718024 8
GCGGT 4649995 1.0187572 5.694026 130-139
GGGGT 5160625 1.0116768 7.7205796 10-14
TGCGT 3848190 0.9944102 8.053257 210-219
ATACG 2356315 0.9850847 5.1196947 120-129
TCCCC 3211400 0.9820846 5.06842 80-89
TATAA 1591045 0.9697187 6.1811066 240-247
AAGAG 2206385 0.966636 13.472995 35-39
ACCCA 2170900 0.9610516 12.62328 240-247
CAGCG 3344030 0.9589308 6.131052 160-169
AGCTG 3165690 0.9580708 5.056427 90-99
CGAAC 2416405 0.9571893 12.8311 70-79
TAGGC 3151795 0.95386565 5.84719 160-169
CGGAG 3697240 0.9486719 6.4823937 210-219
CGTGG 4329670 0.9485779 7.6182346 50-59
ACAAT 1637645 0.9457383 7.47602 100-109
CCATT 2367180 0.9443436 5.8784437 50-59
TGTAA 2122025 0.93627363 6.653505 230-239
GAGTT 2906745 0.928428 5.178956 160-169
ACTAA 1603140 0.9258117 12.001658 240-247
TTCTG 3008680 0.91701376 5.531181 210-219
TGCAT 2565515 0.9157872 6.1299596 210-219
ACGTC 2702540 0.91407126 11.497653 70-79
CCCAA 2058445 0.9112681 9.379111 240-247
TGAAG 2380635 0.8905418 9.668193 35-39
TGAAC 2115075 0.88423157 6.3528633 190-199
GGTGG 4504650 0.88308096 21.722572 25-29
CATCA 1880675 0.8786839 5.520936 230-239
TCGAA 2101220 0.8784392 16.263372 70-79
GAGGC 3370710 0.86488783 13.740342 30-34
CCAAT 1839585 0.85948604 10.279645 240-247
ATCAA 1480555 0.855019 12.84834 70-79
GTATG 2663090 0.85060346 5.161836 220-229
CCAGC 2633850 0.8440866 5.6092052 80-89
CTTCC 2597000 0.83818233 5.287288 230-239
CGGTC 3398150 0.832031 5.437347 130-139
AGCGT 2746410 0.8311791 6.3418894 160-169
GGGCC 3941410 0.81819683 16.592775 15-19
AAAGA 1331010 0.80551547 5.8325424 8
CGTCC 2931910 0.8022807 9.504514 70-79
GACCG 2795150 0.8015346 17.714287 45-49
TAACC 1699160 0.79387707 6.034655 80-89
AACCG 1970355 0.7804995 5.574841 190-199
TGAGA 2071060 0.77473676 5.581078 240-247
TGGGC 3494875 0.7656845 17.967531 15-19
GGTCC 3124610 0.7650553 12.63306 50-59
AAAGG 1701895 0.7456146 8.534399 10-14
AATCC 1572690 0.734788 9.195436 240-247
CTAAT 1489335 0.7343843 9.231425 240-247
AGCCA 1846640 0.7314934 5.675493 80-89
ATGAT 1653090 0.7293715 6.757892 240-247
CATAC 1554300 0.72619593 9.101399 70-79
GTCGA 2360165 0.71428514 11.677258 70-79
GTAGC 2350040 0.71122086 5.539702 80-89
AAGGT 1855950 0.6942689 9.643947 10-14
GGAAG 2167575 0.6874558 6.2267437 30-34
AAAGC 1394815 0.68293124 9.674892 70-79
CATTG 1895250 0.67652917 5.3562603 50-59
AGGAT 1750375 0.6547757 5.828896 10-14
TAGGT 2031595 0.648901 5.5714593 10-14
CAATC 1359510 0.6351866 8.766787 240-247
TATGA 1435790 0.63349503 5.409051 240-247
GAGGT 2329485 0.6308271 6.806614 10-14
ATAGG 1675145 0.62663394 5.276087 10-14
AGAAG 1426625 0.6250165 5.4830413 9
GAAAA 1030285 0.6235194 5.649271 8
CCGTA 1819470 0.61539346 5.157186 80-89
CGATG 2010545 0.6084753 13.504558 15-19
TAAAA 832845 0.5944942 6.158525 8
AATGG 1586870 0.59361213 6.6149535 10-14
AAGAA 953660 0.57714665 5.193195 9
GGGTC 2633765 0.5770258 5.649891 70-79
CATCC 1521595 0.5751562 7.217229 240-247
ACGAT 1368725 0.5722112 6.0344296 10-14
AAATG 1102415 0.56966233 5.3967304 9
GAAGG 1713240 0.5433615 5.4636497 10-14
AAGCA 1080630 0.5290996 6.5220375 70-79
AAGGG 1620440 0.51392955 6.278645 10-14
TGATC 1409460 0.5031214 5.4150267 240-247
GGTCG 2232020 0.4890084 5.7364087 70-79
GATCC 1432125 0.48438302 5.5118446 240-247
ATAAG 910235 0.47035524 5.3718376 9
CGGTT 1720125 0.4444972 8.03441 10-14
TAAGG 1174195 0.43923983 5.8602242 10-14
AAGCT 969675 0.4053838 8.179058 30-34
TAAAG 779370 0.402732 5.4937506 9
Produced by FastQC (version 0.10.1)