FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_5_SL248351.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_5_SL248351.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11034049
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 53210 0.4822345813399959 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 41155 0.37298184918337773 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38821 0.3518291426837057 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 37663 0.3413343551401666 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36366 0.3295798305771526 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35774 0.32421461967406523 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 35558 0.32225704272293876 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34711 0.3145808034747716 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 34693 0.31441767206217774 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32084 0.2907726800923215 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31294 0.28361302365070157 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31201 0.2827701780189666 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30844 0.2795347383358548 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30607 0.2773868414033688 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30224 0.2739157674576214 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30222 0.273897641745111 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29992 0.2718131848064115 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29433 0.26674704815974626 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29175 0.26440883124590076 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28801 0.2610193230064503 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27578 0.24993544980632224 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27164 0.24618342731666318 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26754 0.242467656252025 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26717 0.24213233057058203 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26560 0.24070946213851324 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26301 0.23836218236841256 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26247 0.23787278813063092 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26046 0.23605115402333268 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25547 0.23152878875198035 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25303 0.22931745182570787 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24939 0.22601857214880955 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23562 0.21353901908537837 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23150 0.20980512230822976 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22759 0.20626154551244064 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22571 0.2045577285364602 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22374 0.2027723458541828 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 21955 0.19897500908324767 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21905 0.19852186627048693 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21798 0.1975521406511789 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 21636 0.19608395793783406 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21599 0.1957486322563911 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21597 0.19573050654388066 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20141 0.1825349878362875 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20025 0.18148369651068252 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19672 0.1782845082525916 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19670 0.17826638254008115 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19640 0.1779944968524247 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19285 0.17477718288182334 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18921 0.17147830320492505 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18739 0.1698288633664759 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18045 0.16353924112535662 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 17844 0.16171760701805837 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17721 0.1606028756986669 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17083 0.15482077340783967 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17003 0.15409574490742248 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16704 0.15138595088711315 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16513 0.14965494534236706 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16397 0.14860365401676212 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16155 0.14641044280300006 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15732 0.14257685460704406 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15347 0.13908765494878625 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15335 0.13897890067372368 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15330 0.1389335863924476 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14644 0.13271646700137005 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14069 0.12750532465462136 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 12784 0.11585955436666992 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12734 0.11540641155390917 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12365 0.1120622175957348 No Hit
TAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12210 0.11065747487617646 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 11935 0.1081651894059923 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 11785 0.10680576096771004 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11776 0.10672419526141311 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 11053 0.10017175018889259 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 22584040 7.7309766 67.37199 35-39
TATTA 11353065 7.5722303 131.65439 240-247
TGGAT 17163900 7.308905 81.149956 35-39
TCACC 17598660 6.5160937 76.27325 160-169
TCTCC 15587470 6.1672482 87.19892 20-24
CTGTG 16441590 6.0142927 87.77218 25-29
TCCCT 14765885 5.8421845 73.05175 15-19
AGTGG 17745225 5.840858 40.03105 100-109
CTGAG 16759275 5.73704 50.463417 15-19
TGTAT 10356260 5.7053266 60.999626 240-247
TTACT 9617985 5.5106034 116.451225 240-247
ATTAC 9612205 5.1538296 109.37581 240-247
TGGGT 14146470 4.975667 46.33968 100-109
CCTGA 13850760 4.9311 51.91027 15-19
TTCAC 10311350 4.7492704 76.879906 40-44
CTCTG 12481750 4.7484703 74.004166 35-39
ACACG 14169765 4.7209005 50.194008 230-239
GACAC 14059405 4.6841316 47.189396 230-239
CTCCA 12635685 4.6784987 41.88798 80-89
TGAGA 11424730 4.552755 96.40372 15-19
TACTG 9986830 4.4228435 81.88438 240-247
AAGGG 14256140 4.391259 39.8874 90-99
ATTCA 8178325 4.385018 114.695045 40-44
TCTGG 11776805 4.307926 136.65698 35-39
AGCCT 11899025 4.23625 52.560116 30-34
GAAGG 13712950 4.2239423 31.766876 150-159
TGGAG 12773350 4.2043605 39.693436 90-99
CCCTG 13709590 4.192751 86.166374 10-14
CAGCC 14595405 4.177175 56.61139 30-34
ACCAT 9665370 4.1660247 69.95857 160-169
CCAGG 15093340 4.153501 53.996593 80-89
TCAGT 9307780 4.122114 99.28971 7
GAGAC 12812790 4.104576 90.11572 8
GTATT 7360810 4.055115 71.03195 240-247
CACCA 11701400 4.054502 59.18533 160-169
ACTAC 9386830 4.0459666 36.86337 130-139
TCCAG 11332100 4.034415 41.302673 80-89
TACTA 7465060 4.0025826 30.160065 130-139
GGGTC 14056980 3.9745834 129.28706 8
ACTGT 8789835 3.892733 70.92721 240-247
CACCT 10319825 3.821027 45.612343 40-44
CTGGG 13482690 3.8122036 136.88928 3
TGAAG 9539245 3.8013887 40.706894 150-159
GACTC 10642860 3.7890344 52.054222 20-24
AGACT 9107535 3.7745628 70.83157 15-19
GATTC 8450465 3.742437 59.598507 40-44
CAAGA 9568660 3.7111533 45.683983 180-189
GGACA 11439330 3.6645892 29.212524 220-229
GCTGG 12721785 3.5970592 33.839375 90-99
ACTGG 10430540 3.570586 45.136997 60-69
CCTGT 9194345 3.497833 61.254173 25-29
ACTCT 7520725 3.463946 68.66087 20-24
GAGTG 10346960 3.4057121 40.19045 90-99
TATCT 5908660 3.3853538 50.161854 200-209
AGACA 8661060 3.3591454 40.1267 170-179
TCCTG 8811660 3.3522465 65.093956 20-24
AGCTG 9767680 3.3436756 38.456654 60-69
GGATT 7812775 3.326915 56.90328 40-44
CTCCT 8312065 3.2887034 94.82284 20-24
CTGCA 9074235 3.2305775 45.95643 25-29
GGTCC 10970265 3.225925 144.02872 9
CATCT 6877940 3.167888 32.76941 170-179
GTCTC 8271985 3.1469364 33.851402 30-34
CCATC 8475840 3.1382713 35.867935 45-49
GTCCC 9997125 3.0573816 75.37789 10-14
CCTCA 8244845 3.052743 83.748055 5
AAGAA 6685955 3.0186782 39.624725 180-189
GGAGT 9126810 3.0040987 38.103745 90-99
AGTAG 7500210 2.9888332 29.842842 50-59
GGCTG 10444070 2.95304 34.98314 90-99
GAGCT 8565230 2.9320526 31.09738 60-69
AGGGA 9506575 2.9282703 41.581276 80-89
TGTGC 7836970 2.8667443 58.58269 25-29
ATCTG 6359055 2.8162193 37.575382 200-209
GTGAA 7035205 2.8035288 39.82109 10-14
GACAA 7204385 2.7941818 25.084715 180-189
GGAAG 9067330 2.7929718 31.410868 80-89
CAGGG 10537920 2.7883441 37.1386 80-89
CTATG 6251565 2.7686155 29.024443 50-59
ACCTT 5983725 2.7560246 74.53557 40-44
GTGTA 6470005 2.755123 38.04826 230-239
GAACA 7087330 2.7487826 35.75387 210-219
ATCTC 5945080 2.7382247 48.770412 170-179
ATGAA 5550480 2.6778855 27.930977 210-219
GGGAA 8566680 2.6387587 34.868656 80-89
CACGG 9488085 2.611004 45.000637 230-239
AGAAC 6721180 2.6067734 31.006678 190-199
GAGTC 7585055 2.5965188 29.030275 150-159
TGCAA 6252120 2.5911531 36.746605 200-209
ATCAG 6194960 2.5674636 28.02762 45-49
AGGGC 9661135 2.556346 33.58878 150-159
CGCCA 8874195 2.5397763 40.01996 70-79
CAGTG 7411925 2.5372527 76.737305 8
CCCTC 7945610 2.5271986 26.948622 20-24
TATAT 3773835 2.5170598 25.697111 240-247
AGGCT 7323580 2.5070105 21.067907 25-29
CCAAG 7523625 2.5066245 40.113132 180-189
CTACA 5810905 2.5046504 20.349686 190-199
ACGGC 9057560 2.4925292 49.946564 230-239
GTGGA 7418325 2.441749 27.190737 100-109
CACTG 6781355 2.414275 19.744926 30-34
TCTGT 5082770 2.4053729 25.441097 210-219
GCAGC 8738080 2.404612 34.528313 30-34
CTGTC 6269165 2.3849976 49.707905 30-34
CTACT 5156605 2.375064 23.666998 50-59
TATGC 5335060 2.362725 22.005209 50-59
TGAAC 5694645 2.3601112 38.524437 210-219
ACAGC 7030855 2.34245 27.647243 210-219
CTCTC 5910675 2.3385832 98.438995 20-24
CCGCC 9511740 2.3384647 34.18057 70-79
GGGCT 8265625 2.337089 43.24727 90-99
CAAAT 4657250 2.3368375 36.495125 200-209
CTTCA 5035220 2.3191555 51.369205 45-49
TCCAA 5360900 2.3106866 44.33769 180-189
GTGGT 6564010 2.3087263 32.86985 120-129
GTATC 5204725 2.3050041 23.1716 200-209
GGCCG 10094795 2.294528 25.977213 230-239
CAGGC 8278115 2.2780354 33.946426 70-79
TGGTG 6447800 2.2678525 35.102306 35-39
GCCTC 7359115 2.2506094 72.78563 4
AAATG 4653320 2.2450416 34.713627 200-209
CAGTA 5407025 2.2409089 22.37758 45-49
AATGA 4640065 2.2386465 26.326841 210-219
ACAAC 5546725 2.2373397 20.5007 180-189
GCTCC 7271210 2.2237258 34.314007 80-89
TACAT 4118265 2.208113 19.131039 190-199
GTAGT 5155985 2.1955738 26.568975 120-129
GCCTG 7435920 2.1866121 31.374279 210-219
GCTAT 4916205 2.1772277 35.134056 50-59
GGGGG 10314425 2.1675339 97.84792 5
TATCA 4026165 2.1587312 17.634922 170-179
CCTTC 5393780 2.1340716 55.766136 45-49
GTGGG 7848590 2.1337998 30.515003 100-109
GCTGT 5761265 2.1074564 19.799856 230-239
AACTA 4175085 2.094905 38.59097 130-139
GCAGA 6537280 2.0942173 25.028887 140-149
TCTAT 3648640 2.0904803 20.751478 110-119
CCTCT 5280565 2.0892777 49.19928 35-39
TGCAG 6092445 2.0855677 43.80462 30-34
AACAG 5300065 2.0556016 34.487877 210-219
TGGTA 4803115 2.045311 32.71596 120-129
TGCAC 5721125 2.036815 27.090464 25-29
AGCAC 6097155 2.0313716 21.471207 130-139
CAGAG 6329575 2.0276787 33.28297 170-179
ATGGA 5081145 2.0248363 12.974214 100-109
TGAGC 5899420 2.0194914 18.430563 200-209
CTCAC 5399435 1.999199 54.985077 20-24
ATATC 3717965 1.993482 14.377866 160-169
TGTGA 4670025 1.9886372 19.73295 210-219
GGCTC 6735610 1.9806786 20.482111 40-44
AGAGT 4957260 1.9754679 31.700653 150-159
CATAT 3657120 1.9608585 15.1575 160-169
CCGTG 6653245 1.9564581 20.888975 230-239
GGTCT 5347270 1.9560183 36.573624 15-19
CTGTA 4407805 1.9520738 25.374949 190-199
GCACA 5838645 1.9452448 19.359392 130-139
TCCGC 6360525 1.9452147 42.295906 70-79
TTCAG 4370865 1.9357145 47.965397 45-49
ACTAT 3604030 1.932393 18.174925 130-139
CAGAC 5798075 1.931728 27.11395 140-149
TCTCT 3872480 1.9059376 32.976044 35-39
TGGGG 6972580 1.8956386 127.57195 4
ACCAG 5689120 1.895428 14.240011 190-199
TCCAT 4114035 1.8948698 27.28362 45-49
CTATT 3306145 1.8942485 20.936852 110-119
GGATC 5517080 1.8886087 18.167532 70-79
CCAGA 5663580 1.8869187 34.84616 170-179
GGTGG 6929390 1.8838967 18.6349 40-44
GCCAG 6836430 1.8813013 35.817505 70-79
GTCAC 5277305 1.8788078 38.87442 160-169
GATGG 5698740 1.8757458 21.682005 100-109
AGAGA 5014505 1.8700287 29.131784 170-179
AGGAC 5820975 1.8647488 21.365587 220-229
CAGTT 4203260 1.8614876 25.667933 190-199
GCTGA 5427770 1.8580362 28.221684 200-209
TGGGA 5589725 1.8398635 17.181368 100-109
CCTGC 6015520 1.8397032 50.364235 20-24
CACAG 5518495 1.8385813 16.423887 140-149
ACCCT 4960120 1.8365382 43.461094 10-14
TTCCA 3965805 1.8265972 23.55459 180-189
TCAGC 5126065 1.824964 11.652407 45-49
ATACT 3402735 1.8244634 27.869452 130-139
GAGGA 5921195 1.8238809 27.782104 220-229
TAGTG 4275635 1.8206942 27.194954 120-129
TAGTA 3530010 1.8198912 17.901793 120-129
GCCGA 6579890 1.8107046 20.059427 220-229
TGTCT 3819690 1.8076322 51.191696 30-34
CAAGG 5596340 1.7927871 17.829937 80-89
AGTCA 4296250 1.7805548 29.935062 160-169
TACAA 3540830 1.7766587 18.581425 130-139
CATGA 4281135 1.7742906 24.38225 60-69
AGCTA 4256645 1.7641408 26.988853 50-59
AGTTC 3975595 1.7606621 22.931084 190-199
GCAAA 4531665 1.7575818 33.281464 200-209
TGACC 4861875 1.730908 14.740099 210-219
TTCTG 3651765 1.7281634 43.877953 30-34
CTCAG 4834545 1.7211779 80.04638 6
ACCTG 4818240 1.7153734 30.465036 25-29
GGAGC 6481250 1.7149453 13.65146 200-209
ATGCA 4125275 1.7096953 16.570667 60-69
GTGCA 4964910 1.6995897 51.617313 25-29
CTGAA 4100465 1.6994128 24.020903 200-209
GGATG 5146390 1.6939393 23.153292 100-109
AACAC 4198490 1.6935124 23.1162 190-199
CCTGG 5722440 1.682745 35.265976 2
ATTGG 3906190 1.6633732 21.416487 100-109
CAGCT 4605545 1.6396502 13.622716 50-59
AGGGG 6397005 1.6275362 33.575413 90-99
GCCGT 5495845 1.6161122 30.371063 230-239
TCTGC 4247875 1.616032 34.015934 200-209
GGGGC 7305620 1.5966711 29.049492 90-99
CGATT 3590305 1.5900297 35.261192 160-169
TCTCA 3450320 1.5891716 27.373003 100-109
GGGCC 6983385 1.5873104 28.31946 2
ACAAT 3159040 1.5850906 23.929008 180-189
CTCCC 4931965 1.5686718 14.243523 190-199
TCTGA 3538110 1.5669141 21.798538 220-229
GTTCT 3300205 1.5617908 24.24777 190-199
AGTTA 3028405 1.5612894 16.885506 50-59
CCATG 4349200 1.548387 16.210556 160-169
GGCTT 4223365 1.5448965 22.304554 25-29
GCACT 4323865 1.5393676 26.116768 25-29
CGTCC 5023295 1.5362546 19.361977 180-189
AGTGA 3853635 1.5356734 88.88384 9
TTAGT 2778430 1.5306538 26.704142 110-119
TTCTC 3107060 1.529217 22.987516 190-199
CTTTA 2665910 1.5274274 44.059494 45-49
CGGCC 6440690 1.5225297 32.189198 230-239
GAGCA 4742850 1.5193717 7.856566 200-209
GTTAC 3419030 1.5141776 16.78797 50-59
CATCA 3482700 1.5011338 32.338154 45-49
GTCCG 5078675 1.4934388 34.54958 70-79
ACTCC 3923100 1.4525701 17.52736 140-149
TAGCA 3499805 1.4504731 12.357112 130-139
TACGC 4018355 1.4306008 25.169344 140-149
CTGGT 3897205 1.4255879 32.094765 35-39
GTGAC 4142425 1.4180362 15.694685 210-219
CTCAA 3269440 1.4092132 18.924433 150-159
CAACT 3268220 1.4086875 18.419683 130-139
GGGGT 5178800 1.4079629 121.86529 7
AAGCT 3393540 1.4064322 17.638094 200-209
GGCAG 5291535 1.4001454 13.961617 150-159
GTCCA 3924940 1.3973435 29.329025 180-189
CAACC 4000435 1.3861395 15.490217 140-149
AACCC 3961360 1.3726001 20.770903 140-149
CGTGT 3752280 1.3725747 25.014572 230-239
AGCAG 4271575 1.3683987 13.143614 50-59
GAGAG 4431650 1.3650626 26.4537 220-229
CTACG 3819990 1.3599797 16.623035 130-139
CGACA 4081875 1.3599467 16.783947 70-79
TTACA 2516885 1.3494922 10.021723 120-129
CCTTT 2735010 1.3461032 50.308304 45-49
ATCTA 2508550 1.3450232 21.288132 110-119
GAACT 3245235 1.3449681 14.977589 190-199
CACGT 3761945 1.3393147 16.884203 180-189
CCATA 3101955 1.3370228 15.588871 160-169
CAATT 2492730 1.336541 25.044561 180-189
GGCCT 4531965 1.3326731 69.761734 3
GTGCG 4711455 1.3321545 17.798143 240-247
ACAGT 3179635 1.3177805 12.78179 120-129
TGGAA 3306125 1.3174906 19.791037 120-129
TCAAG 3167315 1.3126746 24.032526 150-159
TGCGA 3828525 1.310582 14.916595 240-247
ACATG 3158660 1.3090876 14.991047 190-199
ATACA 2608645 1.3089224 22.446655 35-39
ACAGA 3370880 1.3073777 20.693 140-149
AATTC 2432720 1.3043652 24.994375 180-189
AAGAG 3481580 1.2983645 19.699682 150-159
GTGGC 4586425 1.2968025 14.576054 40-44
TGTGT 2847325 1.2956314 15.176974 240-247
ACATA 2578420 1.2937567 22.033552 130-139
TGTCC 3395055 1.2915912 54.43571 15-19
ATATT 1932410 1.2888724 27.25479 240-247
CCAGT 3616285 1.2874572 19.513216 190-199
TCCGT 3378810 1.2854111 13.862617 150-159
AACCA 3184560 1.2845315 17.766668 190-199
AGAGC 4005645 1.283208 25.413794 220-229
TTATT 1798340 1.2817124 20.059288 240-247
TCACT 2743265 1.2635114 13.832404 190-199
GATTG 2936485 1.2504437 24.538445 100-109
ACGTC 3511030 1.2499849 18.698235 180-189
ACACA 3080585 1.2425919 7.9446273 130-139
AGACC 3712430 1.2368597 101.397736 9
CCTAC 3340065 1.2366952 17.678463 190-199
CGGCT 4181605 1.229646 21.43419 230-239
ATCCG 3453580 1.2295315 24.304342 70-79
CTTCT 2488685 1.2248683 23.825483 30-34
ATTAT 1833435 1.2228584 9.358282 50-59
AGTCG 3545190 1.2135905 16.639471 150-159
TGGAC 3523255 1.2060817 16.375807 80-89
TACCA 2794740 1.2046052 11.655514 45-49
ATTAG 2330225 1.2013438 26.28888 110-119
TGAGT 2797555 1.1912832 21.571878 90-99
ACGCA 3546775 1.181669 21.776243 140-149
GTCGA 3448340 1.180437 12.979155 150-159
GATCC 3289570 1.1711413 24.106644 70-79
ATTCC 2537810 1.1688815 22.959175 180-189
ATCAT 2175395 1.1663936 16.05408 110-119
CTTCG 3053645 1.1617076 107.934746 3
TCGGA 3393025 1.1615015 96.418335 5
AGCTC 3257270 1.1596421 12.654936 210-219
GAAGT 2904335 1.1573774 13.871654 140-149
GGGTG 4234260 1.1511704 14.868914 60-69
GTATA 2217675 1.1433189 10.722961 240-247
CGCTG 3877695 1.1402779 12.744027 190-199
AAGGC 3558260 1.1398884 29.642015 25-29
TCGAG 3319475 1.1363237 12.672209 150-159
GATCT 2564485 1.1357272 9.758516 220-229
GGACT 3311320 1.1335323 13.346607 90-99
TTGGG 3208735 1.1285923 16.014132 100-109
AGGCC 4089960 1.1255066 14.669812 2
GCAAG 3505075 1.1228505 17.282646 25-29
ACAAG 2893010 1.1220384 18.163433 80-89
GAACC 3356170 1.1181655 11.006255 180-189
GAAGC 3474740 1.1131327 13.714883 200-209
CACAT 2576475 1.1105274 14.63288 130-139
TTCGG 3033680 1.1097126 103.8797 4
GCCCC 4501380 1.106666 12.375982 80-89
CCCCT 3466105 1.1024371 9.90369 70-79
CGCAG 4002660 1.1014826 17.06192 140-149
GACCC 3836005 1.0978568 42.57783 10-14
GCTTC 2882130 1.0964575 28.003853 2
CTTGA 2475265 1.0962145 22.786337 90-99
TTGAG 2572915 1.0956248 21.306866 90-99
TAGAC 2638270 1.0934151 18.289362 170-179
AGGTC 3183000 1.0896057 18.05417 10-14
CCGAT 3054980 1.0876234 23.592371 160-169
ATCAA 2150290 1.0789367 13.045069 110-119
CTATA 1999210 1.0719275 13.998836 50-59
ATGGT 2512845 1.0700451 10.178162 35-39
GCACC 3731660 1.0679935 11.271546 130-139
ATGGG 3242825 1.0673791 20.232552 100-109
ATGAG 2662655 1.0610679 20.940771 60-69
GAGCC 3854355 1.0606711 18.63285 220-229
ACACC 3045610 1.0552953 13.638334 40-44
CTCCG 3434100 1.0502375 10.173681 140-149
AGATC 2527505 1.0475091 13.116376 210-219
TAGTT 1891005 1.0417662 15.626814 50-59
GGAGA 3375560 1.0397596 86.37076 7
GTAGC 3030680 1.0374634 11.523441 50-59
GCTCT 2720605 1.0350081 17.889748 210-219
TACAG 2493615 1.0334636 9.1864805 120-129
CAGCA 3095240 1.0312324 12.476249 45-49
CGTGA 3010075 1.0304099 21.032001 150-159
TGGCT 2812900 1.0289519 32.005444 40-44
GACAG 3176850 1.0177037 16.173468 70-79
AAACT 2027290 1.0172199 17.952753 130-139
CGAGG 3831065 1.0137036 15.154018 220-229
GGGAG 3976475 1.0117012 13.870335 5
AGCCG 3674875 1.0112804 19.670797 220-229
TTTAG 1831170 1.0088027 31.83764 45-49
ACCGC 3524050 1.0085759 11.239735 220-229
TGAGG 3048140 1.0032983 11.922851 220-229
GTAGA 2513015 1.0014365 17.971302 170-179
ATGAC 2395970 0.9929953 11.440541 160-169
TTTCA 1723080 0.9872349 7.3404484 100-109
ACTCA 2286445 0.9855169 12.791517 190-199
AACTC 2284440 0.9846527 16.679327 190-199
ACCAA 2440120 0.9842524 17.743921 130-139
GCCAA 2952730 0.9837526 15.055306 180-189
CGAGT 2868880 0.982076 15.192356 160-169
AAGGT 2462765 0.9814118 21.206985 15-19
TTATA 1465845 0.9776845 13.475572 110-119
GGTAT 2286340 0.97359246 9.568609 110-119
CCAAC 2797605 0.9693622 14.672046 130-139
CCGAG 3517225 0.96789694 18.56045 220-229
CATAC 2214225 0.95438826 14.862394 130-139
TAACA 1882140 0.94438887 9.2045765 120-129
TATGG 2217030 0.9440781 13.0445595 35-39
GGGAC 3564835 0.94325894 10.242252 90-99
CAGAA 2431415 0.9430112 18.750933 140-149
CCGTC 3077185 0.9410835 10.317263 140-149
ACAGG 2926490 0.93750083 15.707726 70-79
GCCAT 2631435 0.93683434 7.9792786 60-69
GATAC 2258965 0.9362144 24.283617 35-39
TACAC 2166775 0.933936 23.067858 35-39
ATGAT 1809985 0.933135 8.951533 120-129
AATCA 1855680 0.9311123 14.645063 110-119
AGCCC 3252885 0.93096906 11.636452 80-89
CGGAC 3373500 0.9283457 11.001087 220-229
TGGTT 2034765 0.92588854 9.647552 50-59
AACTG 2212480 0.916949 11.974087 60-69
GCAGT 2676880 0.9163505 6.1975822 50-59
TCATA 1704545 0.91393536 9.072758 110-119
AAGTT 1766805 0.9108736 17.957787 140-149
GAGAT 2265635 0.90285546 12.634018 210-219
TTTAT 1262115 0.8995342 10.561697 240-247
CCCAG 3136655 0.8977042 15.019297 80-89
CCCCA 2984385 0.8882984 17.049553 80-89
GACTG 2587910 0.88589424 13.001149 90-99
GGTAG 2688855 0.88503927 12.697938 120-129
GTTAT 1604350 0.88384616 10.835162 110-119
TCCTT 1782890 0.8774937 11.456091 1
AGAAG 2349880 0.87632656 16.563503 140-149
CGTAT 1965700 0.87054473 7.8558073 240-247
CGGAG 3285775 0.86941934 74.46821 6
TATTT 1216460 0.86699504 10.619371 240-247
ATAGT 1658665 0.8551221 15.971499 120-129
ATCCA 1977095 0.85217905 10.094817 180-189
GCCTA 2385730 0.8493593 17.028381 190-199
GATCA 2043365 0.84686023 9.440347 110-119
CATGG 2471650 0.8460961 8.879441 200-209
ACGCT 2371915 0.844441 15.73115 190-199
GTTTC 1783420 0.84398663 5.1292777 100-109
TTACC 1828185 0.8420377 10.161349 45-49
GGTTC 2294995 0.83950347 11.863062 160-169
TCTTC 1692335 0.83292484 48.16621 2
GATGA 2077850 0.82802314 6.669812 60-69
GTCTA 1868140 0.8273386 10.640284 30-34
GCTTG 2256180 0.82530504 17.494293 90-99
AGTCT 1856735 0.8222877 14.773506 8
ACAAA 1747880 0.8207342 13.660713 130-139
ATATG 1575425 0.8122079 6.0808506 50-59
TGGCA 2360930 0.8081944 5.6917634 100-109
ACATC 1870570 0.80626404 5.833529 110-119
GGTGC 2848745 0.805477 11.86335 60-69
CCTCC 2524155 0.8028384 10.786026 140-149
TCATC 1739615 0.80124354 10.295629 110-119
CTATC 1735965 0.7995624 8.057555 110-119
GTCTG 2178450 0.7968716 14.173165 1
TATAC 1472755 0.7896552 9.29315 130-139
ATATA 1262275 0.7878736 11.106871 130-139
GTTCA 1755815 0.7775935 14.45727 160-169
TTATG 1405255 0.77416354 11.368877 50-59
GGTTA 1811895 0.7715595 8.631505 50-59
CATTA 1424445 0.7637526 12.501278 110-119
TGATG 1767665 0.752725 11.764871 120-129
CCAGC 2629935 0.752682 6.14147 80-89
CTGAC 2105695 0.7496622 9.961819 220-229
GGGAT 2272700 0.7480615 13.8160515 100-109
CCCCC 2879780 0.73632187 13.566374 80-89
TCAAC 1702530 0.73383445 7.8230505 110-119
CATCC 1979075 0.7327739 6.535635 110-119
GACCG 2658120 0.7314819 9.628398 210-219
GCGGA 2762050 0.7308412 9.345726 220-229
TTTAC 1267370 0.72613686 13.809701 45-49
AACGC 2167155 0.7220248 14.772674 180-189
TGACT 1629835 0.7218011 5.5502563 2
ATCTT 1250870 0.7166832 13.522339 1
ATGCC 2004390 0.71359605 8.523306 50-59
GGGCA 2695085 0.71312225 13.964494 150-159
CACAA 1762200 0.71080506 8.61115 130-139
TGCCA 1994795 0.71018 7.686216 60-69
ACGCC 2478325 0.7092916 13.139089 180-189
TTGGA 1664265 0.70869416 6.3724766 60-69
GACCA 2124105 0.7076821 6.344317 170-179
ATAAA 1211125 0.7074301 7.8447475 130-139
CAACG 2115650 0.7048651 15.052551 180-189
TATTG 1275160 0.7024934 6.3919263 240-247
TCCGG 2382620 0.700635 11.860538 70-79
GGATA 1750935 0.6977476 15.791343 35-39
GAAAT 1441785 0.69560385 7.6010466 110-119
GATTA 1347565 0.694735 5.5664344 50-59
CGCCG 2934850 0.693776 11.111568 220-229
CAAAC 1719890 0.69373876 14.397809 130-139
TATAG 1317460 0.6792144 6.360916 120-129
AATAC 1353585 0.6791794 11.445497 130-139
TTTCT 1097800 0.67211926 9.2802105 4
GCCGC 2787695 0.65898967 12.19779 220-229
CACGC 2294320 0.6566297 12.669393 190-199
GCGAC 2382470 0.6556264 7.3913426 70-79
AAATA 1118470 0.6533094 12.69834 130-139
GACGA 2025805 0.64896655 10.249427 220-229
CCCGT 2090910 0.6394549 10.099095 140-149
CAGTC 1786360 0.63597375 5.335984 210-219
TCCAC 1714965 0.6349843 10.548133 180-189
AGGCA 1978375 0.6337723 6.4760756 80-89
TCAAT 1179305 0.63231456 8.778115 110-119
TACTT 1093550 0.6265471 11.393416 1
CCGGC 2615960 0.6183928 9.603639 70-79
AGATG 1544670 0.61555094 5.915814 120-129
AAGCA 1583550 0.6141713 6.1037025 120-129
ACTTC 1332735 0.61384 30.078695 4
AATAA 1049455 0.61299706 7.2764945 130-139
TAGCT 1367125 0.60545534 12.626836 50-59
CACCG 2106275 0.60281163 9.302409 45-49
CCCTA 1623995 0.60130167 11.962253 110-119
CACGA 1804120 0.60107356 9.070696 180-189
AATGG 1502575 0.5987761 7.0414553 120-129
GGCCC 2514610 0.5944345 10.514474 70-79
ACGAC 1778355 0.5924894 9.571681 220-229
TTAGC 1335675 0.5915271 10.436968 45-49
AGTAA 1204815 0.58127517 5.0875444 50-59
CTTTG 1212835 0.57396275 7.077921 45-49
CGGTT 1567570 0.5734132 11.741822 160-169
CCGTA 1604410 0.57119644 6.7965198 230-239
GTTCC 1488900 0.56642675 6.7523255 140-149
ACCCG 1975665 0.5654313 8.834152 140-149
ACCTA 1306785 0.5632582 7.1602445 130-139
GGGGA 2184725 0.55584127 8.590685 4
TGATT 1003280 0.55271304 6.0108 50-59
CGGCA 1999445 0.55022264 11.07556 70-79
TCAGG 1600625 0.5479265 5.546576 150-159
ATTTC 954045 0.546618 6.28021 240-247
GGTGA 1644170 0.54118025 6.2982173 120-129
TAATA 865045 0.53993475 7.914892 130-139
CTGCG 1833085 0.5390383 12.049819 25-29
TTCAA 997575 0.53487533 5.3716106 200-209
CCACG 1863365 0.5332912 7.917492 180-189
ATTAA 846945 0.5286373 6.2501993 110-119
GCTAC 1480365 0.5270344 8.058747 50-59
AAATC 1046090 0.52488965 8.117385 110-119
CTGTT 1100790 0.52093846 5.868641 240-247
GTCCT 1367545 0.5202593 6.4971175 45-49
CCTAT 1125800 0.5185285 5.026722 110-119
ACCCC 1737210 0.5170784 9.326394 140-149
TATGT 931420 0.5131249 6.916494 140-149
GAAGA 1374485 0.5125783 6.017347 15-19
GGAGG 1998540 0.5084718 5.3954844 6
AAGAT 1053440 0.50824285 5.556639 15-19
TGACG 1463175 0.5008746 9.104778 220-229
ACATT 932220 0.4998336 6.2005506 110-119
GTAAC 1201795 0.49807668 7.3487244 120-129
AGTTT 894935 0.4930251 5.1688323 140-149
GGGTA 1487250 0.48952985 5.327193 100-109
CGCGG 2108540 0.47926718 6.6029525 220-229
GGGTT 1352855 0.4758329 5.7259912 100-109
CCGGT 1598675 0.47010753 9.104758 160-169
AGTAT 908715 0.46848658 5.142008 110-119
TGGCC 1579325 0.4644175 15.220778 2
CAATC 1077435 0.46440235 6.592281 110-119
TATCC 1000845 0.4609759 5.317537 110-119
AACAT 915475 0.45935184 5.9658027 110-119
CCATT 993970 0.45780936 5.1106734 110-119
CATAA 903395 0.45329052 6.0997033 110-119
GGTTT 989065 0.45005885 5.4584413 100-109
CCGCG 1871890 0.4425004 6.838681 220-229
TAAAT 704825 0.43993035 7.841899 130-139
ATGCT 992915 0.43972987 6.887121 50-59
TGCTA 969675 0.4294376 6.151741 50-59
TGCGC 1445525 0.42507216 9.102739 25-29
GGTCA 1230860 0.42134848 5.37148 160-169
ATTCT 735010 0.42112237 7.745958 2
GCGCT 1431675 0.42099944 8.332492 25-29
TTGGT 910045 0.414102 5.1261616 120-129
GCATG 1209575 0.41406214 8.435897 60-69
GCCTT 1074405 0.40873918 8.418089 1
ACGAG 1271975 0.40747708 7.9916663 180-189
CTAAC 931560 0.40152645 5.66086 110-119
CCTAA 915045 0.39440805 7.8477483 110-119
CAATG 951560 0.3943683 5.297889 120-129
ATGGC 1151840 0.39429826 7.0347486 1
GGTAA 978640 0.38998803 7.66727 120-129
CACTT 828300 0.38150403 6.7250037 3
TTTGA 685975 0.37790778 6.1930714 45-49
AGCTT 850770 0.37677845 7.494824 1
CATGC 1055140 0.3756473 6.850126 60-69
CCGGG 1643480 0.37355992 5.1316314 80-89
GAGGC 1361210 0.36017752 5.661648 35-39
TTCTT 585795 0.35864833 20.45319 1
CGAGC 1245465 0.34273663 6.736504 180-189
TGGGC 1187990 0.3359018 9.452408 1
ATGTG 788045 0.33557332 5.212475 140-149
TGCTG 890105 0.3255982 14.052969 1
CGCTT 816580 0.310654 6.329662 1
CTCTT 619380 0.30484328 8.810787 1
AACCT 706670 0.30459306 5.1138153 2
GCGAG 1139305 0.30146122 5.564724 240-247
AACTT 529455 0.28388086 10.695212 3
GACTT 638765 0.28288832 6.889767 1
TTCCT 558160 0.27471235 6.27292 4
ATTGT 497445 0.27404547 5.2610965 240-247
CCCTT 651170 0.25763816 5.5183825 3
GAGGT 779620 0.2566127 6.7955165 7
TAACT 476395 0.25543135 7.0839047 2
GTCTT 525830 0.24884407 12.151217 1
AATCT 457540 0.24532178 7.6117544 2
TTGGC 657840 0.24063624 7.3527303 1
TGTAA 464125 0.23927893 7.419243 25-29
CGGGG 1072335 0.2343629 8.494193 3
CCTCG 722515 0.22096395 5.127552 5
TGCTT 440300 0.20836781 11.032319 1
TCGGT 511940 0.18726638 5.6786523 7
TAGGC 443445 0.15180025 7.07492 1
TTGCT 244470 0.115693115 5.559847 4
Produced by FastQC (version 0.10.1)