FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_10_SL248339.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_10_SL248339.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8121416
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 9147120 8.180589 58.08057 110-119
CACAG 12306400 7.219828 40.46094 110-119
ATACA 8394110 7.101166 36.358757 110-119
TCCCT 14452065 7.0945816 108.050995 30-34
CAGTA 10464850 6.4904385 39.02717 60-69
CCCTG 14561385 6.054452 70.52235 30-34
AGTAA 7931870 6.0082917 45.353085 110-119
GGAGA 12735315 5.99027 90.07493 35-39
GGTGA 14126460 5.7514963 99.44735 45-49
GTAAT 8631675 5.6595416 38.408382 110-119
AATAC 6134970 5.190001 38.094463 110-119
GTAGT 10620680 5.1053386 24.80795 80-89
ACAGT 7970215 4.9432325 34.507614 110-119
AGACG 9382345 4.928646 96.95102 40-44
GTGAC 10794260 4.9081736 81.80825 45-49
ACTCC 8546285 4.846894 122.51671 30-34
GAGAC 8922900 4.687295 135.55328 40-44
CCCCA 8619920 4.6242805 98.32565 60-69
ACCAG 7799080 4.5755067 91.93978 45-49
AAAAA 3794350 4.529143 26.357594 130-139
CTCAG 8876865 4.5078163 33.89388 140-149
CACTC 7743525 4.391621 99.00599 25-29
GCACA 7399085 4.3408403 32.79992 110-119
GTGTC 10967115 4.3164845 23.793089 120-129
GGCCC 12251325 4.3144994 59.265945 60-69
CGTGT 10929175 4.3015523 23.204958 120-129
CTCCC 9065525 4.209637 70.40293 30-34
TGACC 8187505 4.157748 138.85971 45-49
TAGTA 6330235 4.1505547 30.609776 80-89
ATGCA 6472330 4.014225 95.21794 25-29
AGTAG 7156105 3.9740944 27.981415 70-79
GACCA 6720125 3.942513 103.14323 45-49
CCCAG 8146685 3.9132924 84.552605 60-69
TGAGG 9287235 3.7812371 67.491875 35-39
CAGGG 9701070 3.7361293 36.26925 50-59
CCACT 6585420 3.73482 27.37233 240-247
AGGAG 7692140 3.6181276 90.41485 35-39
ATGGT 7524420 3.616973 25.200104 190-199
GGGTT 10204435 3.596228 43.90083 50-59
TGCAC 7072260 3.5914085 116.763824 25-29
GACGG 9297175 3.580579 104.927704 40-44
ACGGT 7859740 3.5738409 93.25654 40-44
TACAC 5157365 3.5723045 26.79233 110-119
CTGAG 7836050 3.563069 103.32024 35-39
TGTCC 7948025 3.4936275 20.427267 120-129
AGATA 4486910 3.398778 18.140568 150-159
TGGTG 9615520 3.3886836 17.995565 190-199
GCCCC 8601645 3.3830502 72.138336 60-69
GAGGA 7191375 3.3825843 118.98442 35-39
CCTGA 6660610 3.382366 83.684616 30-34
CCCTT 6884980 3.3798666 31.747816 20-24
TCTTG 7222080 3.3560221 18.145002 170-179
CTTCA 5570040 3.3395627 16.276468 200-209
CCAGG 7752925 3.3346283 41.100243 50-59
GCACT 6544365 3.323335 90.024 25-29
GGATG 8136045 3.3125377 70.56952 20-24
CCTGG 8656615 3.2228544 30.118448 50-59
CTCTC 6549615 3.215235 13.765485 100-109
CCGTG 8571630 3.1912148 23.403856 120-129
CTTGG 8095110 3.1861084 17.937311 20-24
TCAGG 6946035 3.1583767 20.521004 140-149
GGTTG 8910820 3.1403346 76.522285 15-19
TGTCT 6742945 3.1333737 19.575434 180-189
TTCCC 6379375 3.1316633 51.287247 50-59
CAGAT 4992020 3.0961168 16.882835 150-159
AGGCT 6787605 3.0863388 21.057316 30-34
TCGCA 6049235 3.0719 23.859299 100-109
CGGAT 6747215 3.067973 80.11741 20-24
GGTTC 7716815 3.0372174 49.449677 50-59
CGCAC 6231950 2.993542 24.44564 100-109
TCTCA 4983230 2.987736 20.814081 130-139
GATGC 6535910 2.9718926 97.90054 25-29
AGGGT 7257055 2.9546626 50.89345 50-59
GTTGG 8341395 2.9396589 54.597893 15-19
ACAGA 4093320 2.9329584 14.312134 140-149
TGGCC 7860025 2.9262843 43.994156 60-69
CCAGT 5650095 2.8692102 37.87807 60-69
AGTCA 4603670 2.855257 32.307674 70-79
ACCAC 4328170 2.8358264 18.898817 230-239
GACTC 5552075 2.819434 19.690481 190-199
TCTCT 5364245 2.7838812 14.846755 180-189
GCCGT 7438675 2.7694159 22.170101 120-129
TTCTT 4989745 2.737573 20.43923 170-179
CGGTG 8204805 2.735151 67.079124 40-44
TCAAA 3216525 2.7210839 55.947437 70-79
GCCCT 6544130 2.7209718 17.316742 20-24
GTTCC 6185900 2.7190692 55.904778 50-59
CGGCC 7570850 2.6661956 12.532238 120-129
GATGG 6422695 2.6149585 17.655714 15-19
TCACA 3743865 2.593228 11.550851 140-149
GCGGA 6657075 2.5638094 88.35781 20-24
AGGGA 5427570 2.552949 9.375219 150-159
AGATG 4566055 2.5357275 16.356009 180-189
GGCGG 8905195 2.5143845 43.589024 20-24
TTGGG 6951885 2.4499702 82.14896 15-19
TGTTC 5237750 2.4339259 12.70261 140-149
CTGGC 6487495 2.4152918 36.159912 60-69
GGCTG 7207160 2.4025764 11.598076 140-149
TCCAT 4000140 2.3983169 15.056449 70-79
TCCTC 4882065 2.3966272 16.47162 130-139
CACTA 3432250 2.377385 14.772628 240-247
GTTCT 5098895 2.3694015 18.035036 160-169
ACAGC 4035150 2.367312 10.918849 120-129
ACTCT 3934940 2.3592257 13.178542 200-209
TGGAG 5787520 2.3563511 14.433343 25-29
CAGCT 4582085 2.326857 12.008335 150-159
TACCA 3344050 2.3162923 15.532551 230-239
GAGAT 4168740 2.3150814 15.9052305 180-189
TCAGC 4510910 2.290713 14.6588545 140-149
CACGG 5237215 2.2525902 9.326151 120-129
TGTAG 4658875 2.2395115 8.447071 210-219
AATAA 2157350 2.2289999 8.8779955 110-119
CTGGA 4902005 2.2289524 13.988926 180-189
CTACT 3673820 2.202669 7.6250353 180-189
CAGAG 4172940 2.19209 9.722589 140-149
TGGGG 7317680 2.1842728 54.165524 15-19
TCATT 3443805 2.1828058 15.973975 150-159
CATTT 3438350 2.1793482 17.11776 150-159
TTCAC 3622945 2.1721666 17.790155 200-209
TCTGC 4937135 2.170163 11.930278 210-219
TTCAT 3387060 2.146839 15.546015 140-149
ACTAC 3080670 2.1338594 11.669023 230-239
TCTCG 4810580 2.1145344 17.38715 100-109
GTGAA 3807195 2.1142998 17.039604 190-199
GAACT 3406680 2.1128678 11.078081 160-169
TTGTC 4532285 2.106104 13.105879 170-179
TAGTC 3905150 2.0964742 27.144089 70-79
TTGGA 4343100 2.0877192 16.02338 170-179
GATAC 3345965 2.0752118 13.901696 150-159
CTCGC 4980965 2.0710266 16.504843 100-109
GATAT 3153230 2.0674825 8.476803 180-189
ATCTC 3433040 2.0583074 9.2193365 100-109
ATTTG 3610345 2.0490177 15.128797 150-159
GTCTC 4643200 2.040961 11.61103 180-189
ACACA 2536825 2.0300198 12.980372 110-119
AGCTC 3977800 2.0199914 12.611939 140-149
GCTGT 5098225 2.0065815 9.954859 140-149
CTCTG 4554335 2.0019 14.197177 180-189
ACACG 3412290 2.0018969 12.786984 110-119
GTCAA 3227120 2.0015025 40.764313 70-79
ATATA 2219600 1.9850657 9.653919 110-119
GTCCT 4498435 1.9773284 13.978476 130-139
GTTCA 3675910 1.973407 14.622363 140-149
GAATC 3172195 1.9674375 13.6144 190-199
TACAG 3159545 1.9595917 13.283971 160-169
GGGGC 6873230 1.9406586 49.19702 15-19
CCTTC 3948420 1.9382968 13.593235 200-209
CAGGC 4487545 1.9301482 14.830067 140-149
TGAAT 2935480 1.92471 13.221029 190-199
ATGTA 2920475 1.9148722 6.767128 150-159
AACCA 2371220 1.8974996 14.690574 70-79
TTGCA 3533135 1.8967584 13.873135 150-159
ACGGC 4408700 1.8962361 14.007341 120-129
GAGTC 4147480 1.8858682 11.78594 210-219
GGGCG 6677965 1.8855255 61.36762 20-24
CAGCC 3917540 1.8818059 10.120836 120-129
AATAT 2101920 1.8798203 12.054008 110-119
ATATG 2859440 1.8748528 8.633398 180-189
TTTGC 4004035 1.8606316 11.965422 150-159
AATCG 2993625 1.856686 12.416589 190-199
TGGAA 3335085 1.8521168 8.989623 170-179
TCGGC 4957355 1.8456213 7.7399435 130-139
CTCCA 3251495 1.8440351 7.8875055 150-159
TACTA 2505050 1.8343498 9.103403 230-239
CATAG 2936400 1.8211943 8.037277 210-219
GTCAC 3570010 1.8129091 8.98138 130-139
GTTGT 4330705 1.8019452 7.397639 210-219
AGTAT 2681695 1.7583106 11.356606 220-229
CTGTT 3783160 1.7579933 12.483674 140-149
TTCAG 3248665 1.7440412 9.647679 150-159
AGAAC 2433205 1.7434478 11.2599945 160-169
GACGT 3811310 1.733011 10.089798 170-179
CACCA 2611090 1.7107921 12.937563 230-239
GTCTG 4346265 1.7106217 8.885704 210-219
CGTAG 3762040 1.7106078 9.267765 210-219
CCAGA 2905455 1.704551 23.383045 60-69
TGGTT 4087290 1.7006637 18.38163 10-14
AGTCT 3146015 1.6889336 12.536893 210-219
GGTCA 3699325 1.6820911 8.049909 130-139
ATTGT 2963480 1.6818956 7.36837 170-179
TGCAG 3679445 1.6730517 13.194784 150-159
AACTG 2676690 1.6601187 10.879704 160-169
GCAGA 3158820 1.659362 13.81034 150-159
TGACT 3081575 1.6543392 11.62295 190-199
TCCCA 2894555 1.6416022 10.062991 240-247
ATAGT 2478580 1.6251339 7.1679773 220-229
ACTGA 2610745 1.6192186 9.028926 180-189
CTGGT 4107580 1.6166792 7.3740807 160-169
GAGAA 2513810 1.6128097 10.293984 160-169
TTGTA 2838375 1.6108934 9.433664 210-219
TAGTT 2825880 1.603802 9.914516 220-229
GTGCT 4073600 1.6033052 8.479512 220-229
CAAAA 1629485 1.5925555 19.145409 70-79
TGTGC 4040760 1.5903797 8.143074 220-229
TCTGG 4010060 1.5782967 11.692387 180-189
GCTCC 3795840 1.5782655 6.134321 150-159
TTGGT 3762800 1.565648 22.067057 25-29
TGCTC 3559100 1.564435 5.047794 150-159
CCTTG 3556225 1.5631714 20.419502 20-24
CAAAG 2180640 1.5624791 28.603651 70-79
TCAGA 2518485 1.5619975 9.616029 130-139
CTGTG 3958170 1.5578736 5.19155 210-219
GGACG 4038990 1.555518 7.5660577 170-179
ATAGA 2045625 1.5495353 9.665991 240-247
AATAG 2041835 1.5466644 8.97045 240-247
GCTCA 3032565 1.5399858 11.279584 140-149
GGCCG 4870815 1.535923 13.122055 120-129
GAACC 2597975 1.5241609 12.292463 70-79
CCACC 2824070 1.5150133 9.642495 230-239
CCCAC 2821480 1.5136237 10.116168 240-247
GTCCC 3579685 1.4883908 17.956978 50-59
GCTCT 3352020 1.4734111 9.878875 130-139
AAATA 1413420 1.4603624 5.1093035 9
CTTGA 2698190 1.4485193 8.666725 200-209
CTGCT 3280250 1.441864 5.9617615 90-99
ACCAT 2071340 1.434736 10.406796 45-49
CGACT 2825135 1.4346495 9.037301 190-199
CTCTT 2764220 1.4345468 8.375021 200-209
CACTG 2813380 1.4286801 7.390703 230-239
GAGGG 4120085 1.4207867 8.644495 200-209
CCTCG 3407865 1.4169501 9.372413 130-139
TGATA 2156530 1.413975 7.9597306 180-189
TGGAC 3097075 1.4082468 10.657502 170-179
TCGAC 2760885 1.4020222 9.750766 190-199
GGGAG 4063550 1.4012909 6.875548 150-159
TATAC 1906500 1.3960553 6.4048944 110-119
AGATC 2236380 1.3870325 7.0075974 130-139
AGCTT 2578420 1.3842211 6.4525156 150-159
AATTG 2084180 1.3665371 8.939934 170-179
CATGT 2507430 1.3461102 6.5930815 70-79
CTGAT 2499935 1.3420866 7.583087 180-189
CAGAC 2284735 1.3403914 13.279271 60-69
GGAAT 2407070 1.3367499 8.511476 170-179
GCTGA 2939320 1.3365152 18.12373 30-34
CCATC 2349690 1.3325906 10.340927 240-247
GCGTA 2928370 1.3315362 9.635502 210-219
ATCGG 2906870 1.3217602 7.58282 190-199
TCCAC 2320840 1.3162286 9.86343 240-247
CTCGA 2576835 1.3085587 8.713147 190-199
CCTCA 2280540 1.293373 7.039407 130-139
TATGG 2681550 1.2890155 6.275339 180-189
ACGTG 2824940 1.2845063 9.690271 170-179
ACGGA 2444340 1.284038 8.816432 200-209
ACTTC 2137665 1.2816547 6.2612615 220-229
GAATT 1940060 1.2720418 9.944219 170-179
AGAGC 2414290 1.2682524 7.547611 140-149
GTGTT 3029130 1.2603782 7.436433 160-169
ACTAT 1707895 1.2506247 6.1911354 230-239
AAAGT 1647750 1.2481499 21.308113 70-79
ACTCG 2448385 1.2433296 8.868985 190-199
AGCCG 2885700 1.2411749 8.858245 120-129
GGAGT 3046770 1.2404726 6.9090753 210-219
GGCTC 3310715 1.2325779 8.192446 130-139
ACTGG 2707050 1.2309015 7.4328756 160-169
TTGAG 2539160 1.2205689 8.29136 200-209
CCATG 2401815 1.2196808 6.716402 70-79
GTGGA 2970055 1.2092385 18.84729 25-29
AAGTA 1595460 1.2085409 15.380228 70-79
ATAAC 1424945 1.2054608 7.38177 80-89
ATAAA 1158875 1.1973635 5.351443 7
TTCCA 1989850 1.193031 7.651642 230-239
GATCT 2220295 1.1919622 5.242934 130-139
GGAGG 3448225 1.1890999 14.013069 30-34
GAAGA 1839500 1.1801859 17.045996 35-39
GCATA 1885455 1.1693841 7.747611 210-219
ATATC 1588515 1.1632072 6.7845955 70-79
GTGGT 3266080 1.1510258 9.346044 50-59
ACCGT 2262205 1.1487844 15.868917 45-49
ACTGT 2138915 1.1482735 5.584927 230-239
CTGAA 1851220 1.1481512 13.286935 35-39
TTGGC 2900660 1.1416543 7.4094005 60-69
ATCCA 1645505 1.1397768 14.960214 240-247
GTCCA 2240465 1.1377445 14.213373 70-79
TGGTC 2884850 1.1354316 10.601557 50-59
GCTTC 2578050 1.1332055 5.76185 150-159
TCTAC 1886950 1.1313363 7.3683376 180-189
AAAAG 1289710 1.1286434 12.534393 70-79
ATACC 1611665 1.1163372 6.3804817 70-79
GTCTA 2075045 1.1139848 7.4393563 180-189
TCACG 2192990 1.1136359 9.036798 200-209
TACTG 2073550 1.1131823 6.7592907 180-189
TATCA 1517695 1.1113485 6.3391438 70-79
TGCCC 2664025 1.1076702 5.9663095 60-69
AGGTT 2291910 1.1017164 22.93463 10-14
TCTGT 2368045 1.1004049 6.2771053 210-219
CTTCT 2109825 1.0949354 5.549899 210-219
GAGCT 2393285 1.0882319 6.6385164 140-149
AGTTC 2015225 1.0818707 5.2274165 160-169
CAATA 1278225 1.0813401 6.1740556 100-109
CGGCT 2882675 1.0732188 7.653278 130-139
TATGT 1883635 1.0690396 7.324765 220-229
GGGGT 3570315 1.0657123 7.9765577 10-14
AGGGG 3086560 1.0643818 5.0100093 60-69
CTCGG 2846660 1.0598104 8.265733 130-139
CTGCG 2842045 1.0580922 6.653548 210-219
CTACC 1861585 1.0557692 8.417062 230-239
TAGAT 1605740 1.0528378 7.6862497 240-247
GCGGT 3128280 1.0428424 6.090081 130-139
CCATA 1496360 1.03647 8.785395 70-79
CTGCC 2491075 1.0357596 5.7053056 200-209
GTCCG 2770260 1.0313667 5.118925 120-129
AAGAG 1604790 1.0296007 12.610589 35-39
AGAGA 1587535 1.0185304 11.516275 35-39
ACCCA 1550270 1.0157404 12.660834 240-247
CATGG 2231335 1.0145929 5.1978765 190-199
CAGCG 2314265 0.9953937 6.236869 160-169
TGCGT 2490120 0.98007226 7.877996 210-219
TGAAC 1579395 0.9795616 6.2473173 190-199
TTCTG 2088760 0.9706242 5.576723 210-219
ATGGG 2379170 0.9686636 27.472197 15-19
AGCTG 2125610 0.9665195 5.0768843 80-89
TATAA 1076805 0.96302444 6.199367 240-247
AACTT 1314030 0.9622127 5.4478455 200-209
CGAAC 1608880 0.9438858 12.971389 70-79
CGGAG 2426330 0.93444145 6.4599605 210-219
TGTAA 1419305 0.9305977 6.661387 230-239
GTATG 1934330 0.9298284 5.177772 220-229
ACTAA 1083010 0.916194 11.503035 240-247
CCCAA 1395975 0.91464597 10.014595 240-247
CCAGC 1895120 0.9103285 5.8538165 80-89
ACAAT 1071320 0.90630466 7.0332437 100-109
TAGGC 1979340 0.9000102 5.5210724 160-169
ACGTC 1769490 0.8985758 9.606837 70-79
TGCAT 1663350 0.89296705 5.9563584 210-219
CCATT 1481140 0.88802975 5.3719645 50-59
CTTCC 1798415 0.88284975 5.4530597 230-239
CCAAT 1273315 0.88197535 10.666494 240-247
TCGAA 1413200 0.8764853 16.314182 70-79
CGGTC 2333065 0.8685992 5.906118 130-139
CGTGG 2591055 0.8637532 6.4576344 50-59
CATCA 1245210 0.8625081 5.408956 230-239
AGCGT 1886455 0.8577752 6.344957 160-169
AAAGA 969610 0.8485193 5.8206267 8
TGAAG 1527725 0.8484117 8.830464 35-39
GGTGG 2743845 0.81901723 19.30076 25-29
GAGGC 2073050 0.7983844 11.862874 30-34
ATCAA 943485 0.7981601 11.39517 70-79
GGTGC 2368555 0.7895807 5.1011915 220-229
GACCG 1817025 0.7815247 14.7754965 45-49
TAACC 1111800 0.77010024 6.003563 80-89
TGAGA 1385875 0.7696363 5.3308744 240-247
GAAAA 871290 0.7624781 5.1429048 8
GGGCC 2414095 0.76124096 14.552428 15-19
AATCC 1098125 0.7606281 9.526436 240-247
AACCG 1295975 0.76031303 5.3536377 190-199
GGAAG 1601000 0.7530573 6.222647 30-34
AAAGG 1169270 0.75017995 8.546418 10-14
ATGAT 1124625 0.73738444 6.788387 240-247
AGCCA 1254275 0.7358487 5.792181 80-89
CTAAT 995920 0.72927314 8.925304 240-247
GTCGA 1593090 0.7243815 12.005476 70-79
AAGGT 1290385 0.71660656 9.970135 10-14
CGTCC 1709325 0.7107172 8.000539 70-79
TGGGC 2121635 0.7072675 15.850907 15-19
GTAGC 1526885 0.69427794 5.388532 80-89
GGTCC 1844860 0.68684065 10.807336 50-59
CATAC 974255 0.67482823 7.526098 70-79
CAATC 951870 0.659323 9.288585 240-247
TATGA 994530 0.6520849 5.627411 240-247
AAAGC 897790 0.64328736 8.645008 70-79
TAGGT 1322160 0.63555956 5.268314 10-14
GGGTC 1852405 0.6175171 5.933131 70-79
GAGGT 1515760 0.6171318 6.6892447 10-14
AGGAT 1108925 0.61583394 5.1715384 10-14
AGAAG 959525 0.6156118 5.2954197 9
TAAAA 591040 0.6106696 5.7458134 6
ATAGG 1071695 0.5951585 5.030673 10-14
AATGG 1033075 0.57371116 6.4844484 10-14
CATCC 1008985 0.5722303 6.942842 240-247
CGATG 1226940 0.55789226 12.03565 15-19
GAAGG 1166650 0.5487535 5.632562 10-14
AAGGG 1102225 0.5184501 6.292893 10-14
GGTCG 1517255 0.5057916 6.0288863 70-79
AAGCA 704950 0.505113 5.7392416 70-79
TGATC 937850 0.5034834 5.302127 240-247
GATCC 959625 0.48731315 5.3212585 240-247
ATAAG 622505 0.4715397 5.4550242 9
CGGTT 1167820 0.45963567 8.459656 10-14
ACGAT 698775 0.43338948 5.4214687 10-14
TAAGG 776590 0.43127397 5.7554345 10-14
TAAAG 560820 0.4248141 5.2632422 9
TGGGT 1144800 0.40344828 5.2093797 10-14
AAGCT 645950 0.40062672 7.6365595 30-34
Produced by FastQC (version 0.10.1)