FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_10_SL248339.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_10_SL248339.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8121416
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37112 0.45696464754422134 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30828 0.3795889780796846 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29099 0.3582995871655879 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27618 0.3400638509343691 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25618 0.3154376034918049 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25257 0.31099256582842205 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25213 0.31045078838468565 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 24149 0.29734962474524146 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 23347 0.28747449952077325 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22506 0.27711916247117496 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22291 0.2744718408710993 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22193 0.2732651547464137 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21019 0.25880954749762847 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20429 0.25154480450207206 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20313 0.25011648215040333 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20239 0.24920531099502846 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20138 0.24796168549917896 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19889 0.24489571769257973 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19758 0.24328269848509176 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19072 0.23483589561229223 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18123 0.22315074120079553 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17969 0.22125452014771807 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17911 0.2205403589718837 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17852 0.21981388467232804 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17053 0.20997569881902367 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16764 0.20641720606357317 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16714 0.20580154987750904 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16628 0.20474262123747877 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16576 0.20410233880397213 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16328 0.20104868412109417 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16283 0.20049459355363647 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15941 0.19628350524095797 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15865 0.19534770783814054 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15576 0.19178921508269 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 15487 0.1906933470714959 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15372 0.18927733784354847 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15240 0.1876520055123392 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15075 0.18562034009832767 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14855 0.18291145287964564 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14348 0.17666869915295558 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14331 0.1764593760496938 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 13916 0.1713494297053617 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13667 0.16828346189876248 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13238 0.16300113182233247 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13189 0.16239778875998964 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13066 0.16088327454227194 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12791 0.15749716551891935 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12392 0.1525842291541278 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12377 0.15239953229830858 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12287 0.15129135116339318 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12255 0.15089733120431217 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12236 0.1506633818536078 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 12123 0.1492719988731029 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 12105 0.14905036264611984 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11904 0.14657542477814214 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 11754 0.1447284562199498 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11252 0.1385472681118662 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 11165 0.13747602634811468 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10993 0.13535816906805415 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10987 0.13528429032572645 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10456 0.12874602162972565 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10262 0.12635727562779692 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10073 0.12403009524447461 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9951 0.12252789415047818 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 9657 0.11890783577642125 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9643 0.1187354520443233 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9614 0.11837837145640612 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9523 0.11725787719776945 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 8429 0.10378731984668683 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 16289940 7.5796247 64.45639 35-39
TATTA 8018895 7.1056304 122.594955 240-247
TGGAT 12321680 7.084842 76.566925 35-39
TCACC 12631635 6.405151 73.92411 160-169
TCTCC 11035870 6.043023 84.85599 20-24
CTGTG 11752795 5.905372 85.416046 25-29
TCCCT 10769715 5.897282 74.592964 15-19
AGTGG 12717710 5.6684766 39.360424 100-109
CTGAG 11744250 5.464538 49.140423 15-19
TGTAT 7310725 5.4228 57.82365 240-247
TTACT 6834540 5.2922845 110.23411 240-247
ATTAC 6805985 4.880306 103.260635 240-247
TGGGT 10033360 4.8292727 44.588787 100-109
CCTGA 9819130 4.7694907 50.75425 15-19
CTCTG 9004350 4.7231226 73.54907 35-39
TTCAC 7256665 4.5471463 72.66913 40-44
ACACG 10091505 4.539176 47.92867 230-239
CTCCA 8909690 4.517855 40.246464 80-89
GACAC 10041020 4.516468 44.858227 230-239
TACTG 7135770 4.283235 77.94421 240-247
TCTGG 8519245 4.2806253 134.08386 35-39
TGAGA 8009250 4.2645597 90.40305 15-19
CCCTG 9919915 4.2106853 85.57257 10-14
AAGGG 10168275 4.1968822 38.62518 90-99
ATTCA 5819600 4.1730075 107.98771 40-44
TGGAG 9278100 4.1353903 38.601074 90-99
CAGCC 10347200 4.067146 54.49723 30-34
TCAGT 6725770 4.0371327 91.93569 7
CCAGG 10721585 4.0369663 52.93341 80-89
GAAGG 9780595 4.0368705 30.552269 90-99
AGCCT 8297225 4.030249 49.84926 30-34
ACCAT 6874250 3.988864 66.60051 160-169
ACTAC 6805075 3.9487245 35.74413 130-139
GAGAC 9159415 3.946554 89.5178 8
CACCA 8384935 3.9372373 56.737396 160-169
GTATT 5222095 3.8735387 67.95559 240-247
GGGTC 9896865 3.8547919 121.46217 8
TCCAG 7894455 3.8346095 39.542866 80-89
TACTA 5345930 3.8333573 27.693178 130-139
CACCT 7442945 3.7741103 45.804733 20-24
ACTGT 6204965 3.7245204 67.40047 240-247
CTGGG 9491560 3.6969268 128.42679 3
GACTC 7531750 3.6584315 50.936783 20-24
TGAAG 6834855 3.6392481 38.034203 150-159
GATTC 6028820 3.6187901 57.901646 40-44
CAAGA 6994920 3.6004605 43.812706 180-189
AGACT 6426755 3.5722752 66.02414 15-19
GCTGG 9166145 3.570179 33.067387 90-99
CCTGT 6753330 3.5423777 61.29012 25-29
ACTGG 7521660 3.4997892 43.916862 60-69
GGACA 8119635 3.4985397 27.327673 220-229
GAGTG 7802525 3.477704 38.43321 100-109
ACTCT 5342815 3.3478959 67.005646 20-24
TCCTG 6251130 3.2789545 62.188984 20-24
TATCT 4215365 3.2641425 47.32217 200-209
AGCTG 7005285 3.259523 37.08875 60-69
GGATT 5641485 3.2437973 55.226955 40-44
CTCCT 5870755 3.2147088 91.50882 20-24
GGTCC 7886685 3.2067752 139.2881 9
AGACA 6220815 3.2020092 37.450672 170-179
CTGCA 6507090 3.1607184 46.109398 25-29
CATCT 4949465 3.1014163 32.002773 170-179
CCATC 6109655 3.0980363 35.74435 45-49
GTCTC 5889460 3.0892453 33.878437 30-34
GTCCC 7267425 3.084789 73.57881 10-14
CCTCA 6072195 3.0790408 78.366646 5
AAAAA 4267135 3.0025537 19.634495 90-99
GGAGT 6693115 2.983223 36.994392 90-99
GTGTA 5155680 2.9644642 35.846767 230-239
AGGGA 7158710 2.9547062 39.868763 80-89
GGCTG 7446040 2.9002047 34.102478 90-99
AAGAA 4920475 2.8982544 37.30747 180-189
GAGCT 6169370 2.8705757 30.502497 60-69
ATCTC 4538260 2.843749 46.93682 170-179
AGTAG 5302625 2.8234057 28.151005 50-59
GGAAG 6802040 2.807493 29.970486 80-89
TGTGC 5574905 2.8011966 56.299458 25-29
GTGAA 5075855 2.702661 37.19406 150-159
CAGGG 7490030 2.7015245 36.193893 80-89
ATCTG 4483410 2.69116 35.63044 200-209
GGGAA 6497555 2.6818192 33.515297 80-89
CTATG 4436885 2.6632333 27.14402 50-59
CCCTC 5976805 2.6484072 28.861403 20-24
ACCTT 4220650 2.644729 68.8477 40-44
GACAA 5070595 2.6099622 23.59305 180-189
GAACA 5017750 2.5827615 33.21872 210-219
GAGTC 5520135 2.5684903 28.88234 150-159
CACGG 6667375 2.5104465 43.297394 230-239
AGAAC 4869725 2.5065694 29.330608 190-199
ATCAG 4488870 2.495113 27.105968 45-49
CAGTG 5344110 2.4865866 71.297165 8
CGCCA 6316455 2.4827921 39.45893 70-79
ATGAA 3900595 2.4810655 26.31712 210-219
CTGTC 4708315 2.4696896 50.919746 30-34
CCAAG 5475850 2.4630468 38.836826 180-189
TATAT 2759235 2.444988 23.595886 240-247
TGCAA 4390800 2.4406013 34.206318 200-209
AGGGC 6764415 2.4398077 31.518183 150-159
CTACA 4202500 2.43855 18.54842 190-199
TCTGT 3722030 2.4126196 25.956327 210-219
GTGGA 5405560 2.4093401 27.02747 100-109
ACGGC 6372970 2.3995955 48.06151 230-239
AGGCT 5150445 2.3964753 19.64251 80-89
CCGCC 6947030 2.3862283 34.012093 70-79
GTGGT 4938355 2.376937 30.789545 120-129
GTATC 3940645 2.3653662 22.363998 200-209
CTACT 3761355 2.3569272 23.232277 50-59
CACTG 4843580 2.352694 20.282515 30-34
GCAGC 6232865 2.346842 33.908463 30-34
CTCTC 4258730 2.3319957 95.88363 20-24
TGGTG 4786905 2.3040404 35.408615 35-39
TCCAA 3964765 2.3006012 43.60755 180-189
TATCA 3200915 2.295251 17.538193 170-179
TATGC 3809120 2.2864187 20.806053 50-59
TGAAC 4092250 2.2746537 36.40681 210-219
GGGCT 5795845 2.2574599 41.90041 90-99
CTTCA 3595535 2.2530217 49.754925 45-49
ACAGC 4969810 2.235429 25.99864 210-219
GGCCG 7059770 2.2251627 25.025955 230-239
GCCTC 5214140 2.213235 69.06278 4
CAGGC 5855705 2.2048311 32.301594 70-79
CAGTA 3961255 2.2018409 21.236437 45-49
GCTCC 5175635 2.1968908 33.51988 80-89
CAAAT 3305075 2.1946204 33.44131 200-209
ACAAC 4049220 2.1757922 18.672226 180-189
CCTTC 3918200 2.1455283 55.467514 45-49
GCCTG 5261615 2.1394055 29.78945 210-219
GGGGG 7341785 2.1233976 91.79261 5
AATGA 3333745 2.1205072 24.681475 210-219
GTGGG 5672400 2.1164076 29.328804 100-109
AAATG 3307080 2.103546 31.704187 200-209
TCTAT 2695225 2.0870311 20.804157 110-119
GCTGT 4150085 2.085274 19.105698 230-239
TACAT 2901350 2.0804446 17.163847 190-199
CCTCT 3782655 2.0713067 48.404358 35-39
GCTAT 3435180 2.0619614 32.893013 50-59
AGAGT 3865910 2.0584207 30.635069 150-159
CTCAC 4057965 2.0576813 57.696743 20-24
TGCAG 4398245 2.0464807 42.794407 30-34
GTAGT 3558120 2.0458834 23.88059 120-129
GCAGA 4706085 2.0277297 23.50888 140-149
TGCAC 4168850 2.0249546 26.924217 25-29
AACTA 3016140 2.0027633 36.700565 130-139
TGTGA 3482955 2.002664 20.092443 210-219
GGTGG 5343225 1.9935905 19.136633 40-44
AGCAC 4390030 1.974643 20.943228 130-139
ATATC 2753380 1.9743412 14.376404 160-169
TGAGC 4224730 1.965745 17.941662 200-209
AACAG 3818435 1.9654441 32.020405 210-219
GGCTC 4828345 1.9632351 20.571516 40-44
TCTCT 2896835 1.9602141 33.601246 35-39
TCCGC 4616800 1.9596833 41.79563 70-79
TGGTA 3353435 1.9281914 29.97385 120-129
CATAT 2676930 1.9195216 15.114478 160-169
CAGAG 4433765 1.9103941 31.025417 170-179
ATGGA 3576055 1.904086 11.876787 100-109
CCGTG 4681295 1.9034437 20.093204 230-239
TTCAG 3169790 1.9026614 46.17218 45-49
ACCCT 3739255 1.8960723 45.4215 10-14
GGTCT 3771125 1.8948596 34.527794 15-19
CAGAC 4210285 1.8937931 25.614939 140-149
GGATC 4054655 1.88661 18.473717 70-79
CTGTA 3130970 1.8793598 23.50047 190-199
TGTCT 2888600 1.8723903 51.369568 30-34
CCTGC 4406285 1.8703264 49.253696 20-24
ACCAG 4148060 1.8658042 14.144994 190-199
TGGGG 4987250 1.860774 119.100044 4
GCCAG 4939075 1.8596952 35.201588 70-79
CAGTT 3096220 1.8585012 25.447569 190-199
GTCAC 3822525 1.8567326 37.952793 160-169
TCCAT 2961860 1.8559502 27.638355 45-49
CTATT 2375860 1.8397328 20.229055 110-119
GCACA 4083805 1.8369026 18.112164 130-139
ACTAT 2557765 1.8340731 16.62274 130-139
GCTGA 3930130 1.8286692 27.118834 200-209
CCAGA 4054400 1.823676 32.63268 170-179
AGAGA 3639995 1.7947556 26.576216 170-179
GATGG 4012220 1.7883074 20.195232 100-109
TGGGA 4006820 1.7859006 15.901189 100-109
ACCTG 3672125 1.7836779 32.264305 25-29
AGGAC 4110430 1.7710774 19.476637 220-229
TTCCA 2810360 1.761018 22.739653 180-189
TACAA 2640270 1.7531799 18.389317 130-139
TAGTG 3040425 1.748214 25.936604 120-129
TCAGC 3596000 1.7467015 10.882529 45-49
CACAG 3865450 1.7386857 15.173557 140-149
AGTCA 3127770 1.738553 29.168215 160-169
GCCGA 4616250 1.7381428 18.867163 220-229
ATACT 2423110 1.7375174 25.804567 130-139
TGACC 3555955 1.7272502 15.04429 210-219
GAGGA 4172115 1.7220105 25.444273 220-229
CAAGG 3991235 1.7197195 16.378357 80-89
CTGAA 3082600 1.7134455 23.755009 200-209
AGTTC 2849725 1.7105432 22.872023 190-199
GTGCA 3635275 1.6914747 48.88372 25-29
GCCGT 4149850 1.687355 29.235981 230-239
GGAGC 4668285 1.6837698 12.565083 200-209
CCTGG 4131980 1.6800889 32.763695 2
TTCTG 2582060 1.6736912 41.402687 30-34
CATGA 3005435 1.6705539 22.404102 60-69
ATTGG 2903700 1.6695985 21.614647 100-109
CTCAG 3431380 1.6667398 74.64061 6
ATGCA 2976100 1.6542484 16.114819 60-69
TAGTA 2406230 1.6528081 15.571291 120-129
AGCTA 2968745 1.65016 25.144314 50-59
GCAAA 3204305 1.649336 30.6545 200-209
CTCCC 3668395 1.6255176 14.697896 190-199
AACAC 2990715 1.6070193 21.707792 190-199
GGATG 3594445 1.6020987 21.451338 100-109
TCTCA 2534745 1.5883131 25.639713 100-109
AGGGG 4582735 1.5833588 32.268837 90-99
AAGAG 3198345 1.5769932 19.739706 150-159
GTTCT 2429710 1.5749378 24.366972 190-199
TCTGC 2998725 1.572945 32.55349 200-209
CGTCC 3700605 1.5707879 19.972277 180-189
TTCTC 2317605 1.568264 23.4705 190-199
CAGCT 3225670 1.5668191 12.782117 50-59
GGGCC 4963150 1.5643308 27.08814 2
GGGGC 5180520 1.5641333 28.371342 90-99
GCACT 3199960 1.5543312 25.963512 25-29
ACAAT 2296505 1.5249145 22.448282 180-189
CGATT 2534380 1.5212578 33.505455 160-169
CTGGT 3019930 1.5174102 31.946184 35-39
TCTGA 2524275 1.5151922 20.289026 220-229
AGTTA 2187270 1.5024071 15.968312 50-59
CCATG 3090175 1.5010048 15.351688 160-169
CGGCC 4535660 1.4923906 31.336823 230-239
CATCA 2568505 1.4904051 31.496971 45-49
GTTAC 2469365 1.4822326 17.106281 50-59
CGTGT 2925110 1.4697664 23.904678 230-239
GGCTT 2918995 1.4666938 20.549368 25-29
GTCCG 3590440 1.4598955 33.556164 70-79
GAGCA 3386195 1.4590234 7.193515 200-209
AGTGA 2732340 1.4548463 81.40191 9
CTTTA 1860045 1.4403147 41.03287 45-49
TTAGT 1933550 1.4342291 24.298187 110-119
GTGAC 3077950 1.4321542 16.047575 210-219
ACTCC 2824125 1.4320352 16.780642 140-149
CTCAA 2456415 1.4253635 18.841805 150-159
CAACC 3027995 1.4218279 15.563973 140-149
GTCCA 2918750 1.4177378 29.505198 180-189
AACCC 2983395 1.4008856 20.762306 140-149
TACGC 2874490 1.3962392 23.700268 140-149
GGCAG 3864335 1.393799 12.993719 150-159
CAACT 2384015 1.3833528 18.707233 130-139
AAGCT 2477760 1.3772488 17.491953 200-209
TAGCA 2472155 1.3741333 11.766953 130-139
GGGGT 3680425 1.3731894 113.884544 7
ATCAT 1904155 1.3653951 15.754639 110-119
TGTCC 2582170 1.354446 56.741188 15-19
CACGT 2768780 1.3448919 17.034512 180-189
AGAGC 3121125 1.3448117 23.94861 220-229
GGCCT 3294235 1.3394567 65.99915 3
CTACG 2756545 1.3389491 15.49046 130-139
GAGAG 3220360 1.3291804 24.70725 220-229
TCAAG 2388755 1.3277758 23.90122 150-159
TCGGA 2841245 1.3220166 96.886055 5
ATCTA 1843400 1.32183 21.00654 110-119
GTGGC 3378650 1.3159715 14.913711 40-44
TGTGT 2118980 1.3157247 14.619773 240-247
TGGAA 2463290 1.3115895 19.412777 120-129
ACAGT 2357965 1.3106613 12.361685 120-129
AGCAG 3040380 1.3100208 12.125761 50-59
CCATA 2255780 1.308943 15.736476 160-169
AACCA 2431820 1.3067048 17.531158 190-199
AGATC 2349170 1.3057728 11.9605665 210-219
CAATT 1810895 1.298522 23.826365 180-189
AGACC 2886650 1.2984201 102.91333 9
CCTTT 1914440 1.2954524 48.283546 45-49
TTACA 1806610 1.2954494 9.871126 120-129
CGACA 2876540 1.2938727 15.377801 70-79
ATCCG 2663670 1.2938366 24.716726 70-79
CCAGT 2662955 1.2934892 19.531065 190-199
GTGCG 3302140 1.2861711 16.998598 240-247
GAACT 2308310 1.2830609 13.741957 190-199
ATACA 1919280 1.2744315 20.759806 35-39
ACGTC 2612225 1.268848 18.746662 180-189
TCACT 2016405 1.2635127 12.50324 190-199
TTATT 1319930 1.2630392 18.946896 240-247
GATTG 2194900 1.2620455 24.580622 100-109
TCCGT 2405955 1.2620146 13.824318 150-159
ACAGA 2451370 1.2617815 18.974934 140-149
ACATG 2267370 1.2603047 13.487152 190-199
AATTC 1753950 1.2576889 23.777927 180-189
GATCT 2089720 1.2543511 8.908003 220-229
ACATA 1871520 1.242718 20.16749 130-139
TGCGA 2669385 1.242051 14.249402 240-247
CCTAC 2432720 1.2335646 16.41677 190-199
AGTCG 2646530 1.2314166 16.939037 150-159
ATATT 1377975 1.2210386 25.083801 240-247
CTTCG 2327380 1.2207991 110.31826 3
ATTAT 1373540 1.2171087 9.394999 50-59
CGGCT 2985030 1.213732 20.863247 230-239
CTTCT 1781285 1.2053499 22.545408 30-34
GATCC 2472745 1.2010977 24.54864 70-79
CGCTG 2940480 1.1956174 12.40098 190-199
GTCGA 2561960 1.1920667 13.130609 150-159
ACACA 2202995 1.1837488 7.4282804 130-139
GACCC 2964765 1.1653523 44.982418 10-14
AGCTC 2396240 1.1639366 12.683431 210-219
TGGAC 2500510 1.1634743 15.14793 80-89
TGAGT 2019650 1.1612785 19.897427 90-99
TTCGG 2303805 1.1575822 105.65107 4
GTAGA 2169350 1.1550798 17.820082 170-179
TTGGG 2391845 1.1512465 16.26071 100-109
GAACC 2557080 1.1501789 11.114321 180-189
TACCA 1980470 1.1491911 10.5340605 45-49
GGACT 2460800 1.1449974 13.5918455 90-99
TCGAG 2459605 1.1444414 12.830777 150-159
GGGTG 3064005 1.1431993 14.051246 60-69
GCCCC 3326430 1.1425921 12.707139 80-89
ATTCC 1819305 1.1400067 22.246029 180-189
ATTAG 1653640 1.1358637 23.931383 110-119
AAGGC 2598395 1.119581 27.168217 25-29
ACGCA 2482600 1.1166775 20.218023 140-149
GTATA 1624200 1.1156417 10.078777 240-247
GAAGT 2091940 1.1138626 12.583131 140-149
GAAGC 2577635 1.110636 13.642251 200-209
AGGCC 2940440 1.1071551 13.321398 2
CCCCT 2474440 1.0964594 10.215168 140-149
GGAGA 2651525 1.0943979 85.62121 7
TAGAC 1964490 1.0919507 18.223227 170-179
GCACC 2774405 1.0905281 11.433408 130-139
GCAAG 2526400 1.0885602 16.262892 25-29
CGCAG 2889085 1.0878186 16.059727 140-149
AGGTC 2320075 1.0795189 17.298544 10-14
CACAT 1858925 1.078663 13.760783 130-139
ACAAG 2082315 1.0718195 16.37111 80-89
ATCAA 1612115 1.0704691 12.879915 110-119
GCTTC 2034135 1.0669808 28.056261 2
GCTCT 2027010 1.0632436 18.220818 210-219
CCGAT 2181070 1.0594211 22.675482 160-169
TGGCT 2104045 1.0572097 32.20797 40-44
TACAG 1889885 1.0504819 9.012764 120-129
ATGGT 1820500 1.0467693 10.245936 35-39
TTGAG 1819180 1.0460103 19.649677 90-99
CTTGA 1742065 1.0456718 21.180159 90-99
CTCCG 2452490 1.0410032 9.736627 150-159
ATGAG 1939895 1.0329056 19.597929 60-69
ACCGC 2621885 1.0305773 11.3960905 220-229
ACCAA 1912700 1.0277628 17.776442 130-139
CTATA 1432710 1.0273403 12.762319 50-59
ATGGG 2300905 1.0255483 18.888102 100-109
GGGAG 2952305 1.0200367 14.233145 5
GAGCC 2706730 1.0191569 17.566027 220-229
CAGCA 2258000 1.0156521 11.4407425 45-49
GTAGC 2181700 1.0151337 11.15167 50-59
CCAAC 2157410 1.0130353 15.040544 130-139
CGTAT 1684235 1.0109594 7.492288 240-247
TAGTT 1361295 1.0097536 15.108286 50-59
ACACC 2147635 1.0084454 12.654368 40-44
CGTGA 2154010 1.0022497 20.17769 150-159
CGAGT 2147490 0.99921596 15.263914 160-169
GACAG 2291835 0.9874922 14.794076 70-79
TTATA 1113225 0.9864408 13.381392 110-119
TTTCA 1268770 0.9824644 6.7793875 100-109
AGCCG 2595895 0.97742474 18.506113 220-229
ATGAC 1751230 0.97341126 10.509839 160-169
ATGAT 1411340 0.96943104 9.011842 120-129
GGTAT 1682895 0.96764773 9.419144 110-119
CGAGG 2671365 0.9635152 13.965288 220-229
GGGAC 2668025 0.96231055 10.457066 90-99
TGAGG 2156285 0.961089 11.193097 220-229
CCGTC 2262970 0.96055806 10.516876 140-149
AGCCC 2443390 0.9604169 11.870711 80-89
AAACT 1443305 0.9583766 16.026617 130-139
CCCCA 2331445 0.9566717 17.597557 80-89
ACTCA 1644005 0.95395315 11.424587 190-199
TTTAG 1282570 0.95135856 29.049265 45-49
AATCA 1431715 0.9506808 14.605979 110-119
AACTC 1621295 0.9407756 15.360386 190-199
AAGGT 1764205 0.9393587 19.769642 15-19
TATGG 1633660 0.9393382 13.317629 35-39
CCCAG 2380025 0.93551 15.376826 80-89
CATTA 1303250 0.9345095 11.381625 110-119
CGGAC 2476920 0.9326273 11.253091 220-229
GCCAA 2070650 0.9313819 13.974626 180-189
CCGAG 2471230 0.9304849 17.427698 220-229
GCAGT 1996180 0.9288122 5.8346276 50-59
TGGTT 1487630 0.92370456 9.8542795 50-59
GATAC 1660165 0.9227932 22.875755 35-39
GCCAT 1896395 0.92114455 7.6979604 60-69
CATAC 1578905 0.9161781 13.925102 130-139
TCATA 1274025 0.9135535 8.872831 110-119
CGGAG 2530425 0.9126806 74.99182 6
GACTG 1960025 0.9119895 13.235985 90-99
TCCTT 1346200 0.910939 11.371718 45-49
ACAGG 2100300 0.90496475 14.3442745 70-79
TTTAT 945470 0.90471905 9.830437 240-247
CAGAA 1757305 0.90452874 17.017878 140-149
TACAC 1548880 0.8987559 20.765322 35-39
TAACA 1341955 0.89107877 8.091985 120-129
GAGAT 1662675 0.88529843 11.311224 210-219
ACGCT 1811475 0.87989587 15.112179 190-199
AACTG 1579310 0.87785053 10.696464 60-69
GTTAT 1180425 0.8755915 10.897291 110-119
TCTTC 1287220 0.87102884 48.641575 2
GTCTG 1710255 0.85934395 16.117123 3
GGTAG 1925195 0.8580887 11.850313 120-129
ATAGT 1247540 0.856919 15.534475 120-129
AGAAG 1734705 0.85532296 14.774385 140-149
TATTT 892645 0.85417086 10.112273 240-247
AAGTT 1239135 0.8511457 16.202618 140-149
GATGA 1583910 0.84335977 6.424316 60-69
GTCTA 1404255 0.84290195 10.862449 30-34
AGTCT 1394995 0.8373436 15.665086 8
GATCA 1489575 0.82797176 8.886194 110-119
GGTTC 1645040 0.8265756 11.227359 160-169
ATATG 1202625 0.8260673 5.645895 50-59
CCTCC 1859325 0.82389313 11.260411 140-149
ACAAA 1331835 0.8189373 12.267196 130-139
CATGG 1753895 0.8160782 8.21729 200-209
TTACC 1302265 0.8160208 9.6762495 45-49
TGGCA 1749995 0.81426364 5.745002 100-109
CGCCG 2471595 0.8132411 11.470027 220-229
GCCTA 1672030 0.81216276 15.764702 190-199
ATCCA 1393080 0.8083511 9.1282015 180-189
ACATC 1389180 0.80608803 5.7413955 110-119
GCTTG 1586425 0.79712355 16.291132 90-99
CTATC 1270795 0.7963012 7.824103 110-119
GGTGC 2043185 0.7958129 11.197406 60-69
ATATA 962100 0.7894605 9.719819 130-139
TCATC 1258170 0.7883901 9.866488 110-119
TGATG 1356065 0.77972376 11.58791 120-129
TTATG 1048280 0.7775716 10.486259 50-59
CCCCC 2143695 0.7686817 14.133526 80-89
TATAC 1071540 0.7683594 8.206921 130-139
ATTAA 930400 0.7634487 6.1046944 110-119
CCAGC 1929400 0.7583841 6.0533147 80-89
GGTTA 1318540 0.7581473 8.684238 50-59
CTGAC 1551320 0.7535297 9.4746065 220-229
GTTCA 1239680 0.74411595 13.429327 160-169
CATCC 1465180 0.74295205 6.231372 110-119
GACCG 1969215 0.74146277 9.871483 210-219
ATAAA 973115 0.7394289 7.7332826 130-139
GCGGA 2045475 0.7377675 9.540616 220-229
TTGGA 1278025 0.73485154 6.411471 60-69
AAATA 962610 0.73144656 12.231328 130-139
TCCGG 1788690 0.7272925 12.297517 70-79
TCAAC 1243255 0.7214133 7.4581313 110-119
GGGAT 1617510 0.72094876 12.747217 100-109
ATCTT 930075 0.720198 13.695767 3
ATGCC 1465840 0.7120092 8.110408 50-59
TATTG 954170 0.7077647 6.157868 240-247
TGCCA 1451855 0.70521617 7.428131 60-69
GATTA 1026545 0.7051203 5.649119 50-59
TTTAC 905090 0.70085096 13.070659 45-49
CACAA 1296655 0.6967395 8.136534 130-139
GACCA 1544595 0.6947615 5.978436 170-179
GAAAT 1088570 0.69241065 7.6356773 110-119
AACGC 1538325 0.6919412 13.595181 180-189
ACGCC 1759475 0.6915921 12.322408 180-189
AATAC 1039490 0.6902373 11.121465 130-139
CCGTA 1415390 0.6875038 6.368248 230-239
AATAA 902420 0.6857107 7.3066845 130-139
GGATA 1284865 0.6841319 14.950548 35-39
GCCGC 2077665 0.6836244 12.482264 220-229
GGGCA 1893445 0.6829329 12.979644 150-159
TTTCT 809355 0.67678523 9.743445 2
CAAAC 1247585 0.6703725 12.99531 130-139
CAACG 1487450 0.66905755 13.841923 180-189
CAGTC 1361720 0.6614345 5.1852784 210-219
CCCGT 1556755 0.6607925 10.28204 140-149
GAAGA 1338675 0.6600543 6.126867 15-19
CACGC 1675050 0.6584074 12.312558 190-199
TATAG 957940 0.6579964 5.932821 120-129
AGGCA 1502510 0.6473925 6.533112 80-89
TCAAT 900890 0.645993 8.890365 110-119
ACTTC 1022535 0.6407373 30.67939 4
CCGGC 1943925 0.63961923 9.996004 70-79
AGATG 1191940 0.6346537 5.480085 120-129
GCGAC 1666665 0.6275444 6.8990226 70-79
AAGCA 1218215 0.62704575 6.068553 120-129
TCCAC 1222060 0.61967266 9.798457 180-189
GACGA 1436680 0.6190281 9.460331 220-229
TACTT 799035 0.618728 11.538685 1
CACCG 1557625 0.61225146 9.283299 45-49
TAGCT 1011695 0.60726845 12.16488 50-59
GGCCC 1840795 0.6056859 10.1212015 70-79
GTCCT 1146625 0.60144836 6.8590927 45-49
TGATT 801150 0.59426063 6.1692023 50-59
CCCTA 1170130 0.5933404 11.315848 110-119
GGTGA 1328425 0.5920992 6.106363 120-129
GGGGA 1705655 0.5893127 9.180937 4
CTTTG 899905 0.58331835 7.3992405 45-49
AATGG 1095415 0.5832585 6.4875407 120-129
ACCCG 1479200 0.58142513 8.863625 140-149
ACGAC 1288410 0.5795289 8.915018 220-229
CACGA 1285360 0.57815707 8.300954 180-189
AAGAT 906585 0.5766548 5.5114384 15-19
TAATA 701875 0.57593036 8.014929 130-139
CGGCA 1510860 0.5688796 11.362729 70-79
CTGCG 1396675 0.5678968 12.662539 25-29
ACCTA 969270 0.56243026 6.6888075 130-139
TCAGG 1203505 0.5599846 5.4757643 150-159
TTAGC 928505 0.5573337 9.274261 45-49
AAATC 829555 0.5508372 8.275986 110-119
CGGTT 1095285 0.55034274 11.115605 160-169
ATTTC 710000 0.5497842 6.056792 240-247
GTTCC 1045955 0.54864305 6.1035132 140-149
ACCCC 1325835 0.54403543 9.504588 140-149
TTCAA 758525 0.5439086 5.2190866 200-209
GCTAC 1087075 0.52802986 7.802903 50-59
TATGT 708740 0.52571464 6.3921423 140-149
GGAGG 1506025 0.5203394 5.1940393 6
CCACG 1314890 0.5168403 7.3477197 180-189
CTGTT 787725 0.51060325 5.294866 240-247
GGGTA 1130035 0.5036738 5.3952556 100-109
GGGTT 1024210 0.4929744 5.459924 100-109
CGCGG 1560815 0.49195188 6.7958226 220-229
TGACG 1040800 0.48427883 8.523731 220-229
ACATT 674955 0.4839838 5.570248 110-119
GTAAC 857645 0.47671708 6.470765 120-129
CAATC 820585 0.4761541 6.740466 110-119
TAAAT 578000 0.4742835 7.785604 130-139
AACAT 713150 0.47354257 5.5645027 110-119
TGGCC 1157210 0.4705288 14.591495 2
TATCC 747565 0.46843663 5.2339187 110-119
CCGGT 1134450 0.4612745 8.65767 160-169
CCGCG 1401080 0.46100426 7.0473013 220-229
TGCGC 1117730 0.45447606 9.461904 25-29
ATGCT 750200 0.45030645 6.6516976 50-59
CATAA 672975 0.44686565 5.681027 110-119
GGTTT 712050 0.44212866 5.007311 100-109
TGCTA 733500 0.44028226 5.8890295 50-59
GCCTT 834920 0.4379472 9.21807 1
GCGCT 1075315 0.43722984 8.728007 25-29
ATTCT 564295 0.43695843 7.501033 2
TGGTC 859400 0.43181872 5.4794936 8
GGTCA 914750 0.42562845 5.3200727 160-169
GCATG 892210 0.4151407 7.9519525 60-69
CACTT 648715 0.4064955 7.2774243 3
ATGGC 867045 0.40343153 7.08876 1
CTAAC 690340 0.4005779 5.317389 110-119
CCGGG 1262260 0.3978506 5.3706684 80-89
AGCTT 656800 0.3942432 7.2304955 1
CATGC 811400 0.394125 6.9097056 60-69
ACGAG 913785 0.3937262 7.2333136 180-189
CCTAA 676705 0.392666 7.3430467 110-119
TTTGA 525835 0.39004314 6.0694056 45-49
TTCTT 453795 0.3794648 20.687157 1
GGTAA 701815 0.37368444 6.7495146 120-129
GAGGC 1013140 0.3654221 5.2832513 35-39
TGGGC 907185 0.35334516 8.716316 1
TGCTG 693765 0.3485929 13.170418 1
TCGGT 671700 0.33750597 5.4317255 7
CGAGC 882770 0.33238676 6.1955094 180-189
AACCT 572190 0.33201998 5.4296117 2
CTCTT 480825 0.32536194 9.669248 1
CGGGA 848715 0.30611685 5.1115556 80-89
AACTT 423910 0.3039693 10.65888 3
CGCTT 577875 0.30311736 5.8983455 1
GACTT 502555 0.3016579 6.573852 1
GCGAG 825695 0.29781392 5.165444 240-247
ATTGT 389515 0.28892648 5.08682 240-247
TTCCT 419825 0.28408483 6.539129 4
GAGGT 619495 0.2761183 6.4698405 7
TGTAA 399590 0.27447313 7.809596 25-29
TAACT 377955 0.27101675 7.22777 2
CCCTT 492230 0.26953536 5.752843 3
GTCTT 410920 0.26635832 12.068317 1
AATCT 359280 0.25762564 7.3517604 2
TTGGC 507825 0.25516447 7.3674827 1
CGGGG 837705 0.25292486 9.191608 3
TCCTA 389845 0.2442833 5.102016 9
CCTCG 564615 0.23966092 5.1196637 5
TGCTT 350180 0.22698665 11.344795 1
TGCCT 378175 0.19836715 5.015166 4
TCGCA 382485 0.18578617 6.900157 7
TAGGC 338785 0.1576349 6.3312836 1
TTGCT 200105 0.12970805 5.463353 4
Produced by FastQC (version 0.10.1)