FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_13_SL248342.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_13_SL248342.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10666444
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12195570 8.643022 60.277905 110-119
ATACA 11191175 7.488444 36.656693 110-119
CACAG 16480650 7.487282 41.3256 110-119
TCCCT 18755700 6.860429 101.10018 30-34
CAGTA 13465695 6.479271 38.62785 110-119
AGTAA 10615925 6.3680105 46.91125 110-119
GGAGA 16848145 6.1512284 94.41167 35-39
GTAAT 11514920 5.868213 38.866173 110-119
GGTGA 18891150 5.85959 102.09827 45-49
CCCTG 18398130 5.696054 66.232346 30-34
AATAC 8147285 5.4516616 38.65023 110-119
AGACG 13288270 5.4118805 102.0663 40-44
GTAGT 14566160 5.3379135 32.192287 80-89
ACAGT 10949040 5.268336 35.173992 110-119
GTGAC 14573500 5.0424643 85.028305 45-49
TAGTA 9479350 4.8308496 41.677807 80-89
CCCCA 11803105 4.7980814 101.715126 60-69
GAGAC 11681955 4.757681 141.94724 40-44
CTCAG 12073505 4.6599603 34.222466 140-149
ACTCC 10426080 4.4889 114.511734 30-34
GGCCC 17041075 4.465599 58.128735 60-69
GTGTC 14750635 4.3359942 23.310822 120-129
GCACA 9537280 4.332857 33.88041 110-119
ACCAG 9504070 4.3177695 88.83002 45-49
AGTAG 9988310 4.308433 33.670593 80-89
TGACC 11135435 4.297898 145.59613 45-49
CGTGT 14495390 4.2609644 22.746384 120-129
CACTC 9588710 4.1283746 93.42509 25-29
CTCCC 11721995 4.048292 65.66253 30-34
CCCAG 10976065 3.9998894 87.01496 60-69
CCCTT 10534400 3.8532553 41.894848 20-24
GACCA 8454415 3.8409035 102.71299 45-49
ATGCA 7952230 3.8263643 91.56549 25-29
TGAGG 12116525 3.7582612 68.92775 35-39
TACAC 6890230 3.6982913 26.49037 110-119
AGGAG 10109675 3.6910245 94.592155 35-39
GACGG 12600285 3.6901298 108.410645 40-44
ATGGT 10059205 3.6862953 25.284454 190-199
ACGGT 10567805 3.6564841 96.3603 40-44
AGATA 6053050 3.6309493 19.008926 150-159
TGTCC 11046285 3.6221318 20.138939 120-129
CCACT 8362570 3.6004655 26.455639 240-247
CTGAG 10382345 3.5923147 104.15087 35-39
CAGGG 12029315 3.5229154 33.614887 50-59
TGGTG 13337120 3.5145526 19.17001 25-29
GAGGA 9565075 3.492192 124.62383 35-39
GCCCC 11868625 3.4693904 73.09098 60-69
CTTGG 11752815 3.4547756 24.042654 20-24
AGGCT 9871800 3.415665 29.750689 30-34
CCGTG 12237370 3.3963969 22.944218 120-129
TGCAC 8764100 3.3826435 109.89332 25-29
TCTTG 9441985 3.2791317 17.610619 170-179
GGGTT 12390855 3.2651956 39.483265 50-59
GGATG 10516800 3.2620642 66.7723 20-24
CAGAT 6779230 3.2619536 17.578354 150-159
CTCTC 8874035 3.2459297 13.411558 100-109
CCTGA 8377225 3.2333226 80.42603 30-34
TCAGG 9314030 3.2226753 20.554733 140-149
CTTCA 6952235 3.170231 16.150412 200-209
CCAGG 9635775 3.1478784 38.141026 50-59
TCGCA 8081040 3.1190057 23.33383 100-109
GCACT 8061765 3.111566 84.952385 25-29
TCTCA 6776355 3.0900295 21.138882 130-139
ACAGA 5446640 3.0848 14.142608 140-149
TGTCT 8880570 3.0841568 19.235811 180-189
CCTGG 11057290 3.0688736 27.770466 50-59
CGCAC 8345585 3.0412917 23.895798 100-109
GCCCT 9644825 2.986034 22.543846 20-24
GGTTG 11077255 2.9190404 70.30852 15-19
CGGAT 8392740 2.9039068 75.67671 20-24
GATGG 9269555 2.8751981 23.282501 15-19
GACTC 7409990 2.860003 18.95662 190-199
TGGCC 10279395 2.852975 42.3499 60-69
TTCCC 7744170 2.8326495 46.553917 50-59
ACCAC 5550950 2.8131127 18.080236 230-239
TCTCT 7255345 2.8107588 14.674396 180-189
GATGC 8056615 2.787607 91.71704 25-29
GGTTC 9473065 2.7846363 44.556175 50-59
TCAAA 4153820 2.77948 55.78641 70-79
CGGTG 11122170 2.7672594 68.01078 40-44
AGGGT 8903440 2.7616374 47.18772 50-59
TCACA 5087350 2.7306058 11.470696 140-149
GCCGT 9810645 2.7228763 21.676296 120-129
CGGCC 10342615 2.7102735 12.46713 120-129
TCCAT 5934470 2.7061286 20.874699 70-79
GTTGG 10214110 2.6915874 50.544285 15-19
TTCTT 6545345 2.685627 19.998816 170-179
AGATG 6116305 2.6382532 16.725033 180-189
TGGAG 8411530 2.609059 20.35055 25-29
AGTCA 5418505 2.607215 29.234034 70-79
GGCTG 10462185 2.6030514 15.474822 30-34
GTTCC 7739505 2.5378222 50.410084 50-59
AGGGA 6928235 2.5294864 8.872985 150-159
CCAGT 6542510 2.5251856 33.43656 60-69
TCCTC 6796990 2.4861917 16.630823 130-139
TGTTC 7044410 2.4464717 12.540599 140-149
ACTCT 5356630 2.4426327 13.439899 200-209
GAGAT 5598840 2.4150462 16.258286 180-189
AATAA 2880830 2.4031568 10.487421 110-119
GCGGA 8185940 2.397341 83.17867 20-24
ACAGC 5243290 2.3820655 11.045439 120-129
TGTAG 6450360 2.3637986 7.742922 210-219
GGCGG 11155885 2.349345 40.251 20-24
CTGGA 6744375 2.3335688 14.209349 180-189
GTTCT 6677265 2.318965 16.800034 160-169
CACGG 7064325 2.3078203 9.616914 120-129
TACCA 4269260 2.2915008 15.287436 230-239
CAGCT 5894325 2.2750082 11.932442 150-159
TTGGG 8550910 2.2533062 75.96605 15-19
CAGAG 5522745 2.2492347 9.087591 140-149
CATTT 4634475 2.238281 17.331264 150-159
TCATT 4604780 2.2239394 16.149092 150-159
TTCAT 4588055 2.215862 15.4661 140-149
CTGGC 7959250 2.2090347 32.684868 60-69
GTGAA 5099625 2.1997113 17.505344 190-199
TTGTC 6255985 2.1726573 12.867743 170-179
TTCAC 4743800 2.163181 17.859709 200-209
GATAC 4476090 2.1537542 14.129446 150-159
ACTAC 3992355 2.1428735 12.012366 230-239
TCAGC 5551705 2.1427686 13.860482 140-149
ATGTA 4200535 2.1406693 9.363373 70-79
CTACT 4681140 2.134608 7.415567 230-239
ATTTG 4861255 2.1047108 15.242898 150-159
CTCGC 6758315 2.0923715 16.07594 100-109
GAATC 4331820 2.084336 14.415725 190-199
CACTA 3876075 2.080461 14.312154 240-247
CTCTG 6307270 2.0681849 14.095807 180-189
TCTGC 6295650 2.0643747 11.548426 210-219
GCTGT 6996805 2.0567322 9.821179 140-149
TGAAT 3995150 2.0360014 13.855909 190-199
GAACT 4225315 2.0330894 10.494459 160-169
GTCCT 6200010 2.0330138 14.141644 130-139
TTGGA 5526960 2.025409 15.03228 170-179
ACACA 3205125 2.0249455 13.451969 110-119
TCTCG 6170830 2.0234456 15.605236 100-109
TACAG 4199960 2.0208893 13.280615 160-169
GTCTC 6152255 2.0173547 11.50961 180-189
ATCTC 4412065 2.0119092 7.91903 100-109
CCAGA 4416110 2.0062716 31.459126 60-69
TGGGG 8984370 2.003909 50.111893 15-19
CAGGC 6132915 2.003541 15.117194 140-149
AGCTC 5179010 1.9989209 11.863574 140-149
AATCG 4154110 1.9988279 13.206518 190-199
GTTCA 4867950 1.9899522 14.49373 140-149
TTTGC 5696115 1.9782184 11.687313 150-159
ATATA 2774405 1.9662257 10.272064 110-119
GATAT 3857375 1.9657885 7.9018173 180-189
ACGGC 6008055 1.962751 14.268144 120-129
TGGAA 4544770 1.9603759 8.600825 170-179
TTGCA 4773475 1.9513319 13.760731 150-159
TAGTC 4768770 1.9494084 23.773535 70-79
AAAAA 1973445 1.937717 8.136221 8
ACACG 4255205 1.9331713 13.416754 120-129
AATAT 2718160 1.926365 12.871017 110-119
GTCAA 3995875 1.9226902 37.398064 70-79
AACCA 3025180 1.911259 14.365536 70-79
GACGT 5521535 1.9104633 10.016217 60-69
CCTTG 5822510 1.9092298 27.278841 20-24
GAGTC 5503815 1.9043323 11.804895 210-219
CCTTC 5201030 1.9024241 13.306016 200-209
ATATG 3732660 1.9022315 8.130626 180-189
CAGCC 5174715 1.8857657 10.066678 120-129
CATAG 3901405 1.877234 8.161372 210-219
TACTA 3302060 1.8771542 9.987809 240-247
TCGGC 6749920 1.8733935 7.4853215 130-139
GTTGT 5794165 1.8039165 6.7416935 210-219
CTGTT 5156845 1.7909342 12.236541 140-149
AGTAT 3509915 1.7887166 11.466754 220-229
GGGGC 8457670 1.7811213 45.621628 15-19
CGTAG 5106155 1.766741 9.337727 210-219
AGAAC 3098165 1.7546998 11.149355 160-169
GTCCC 5656150 1.7511417 23.656603 50-59
GGGCG 8227460 1.7326407 56.266174 20-24
GCAGA 4231850 1.723495 14.013682 150-159
ATTGT 3958220 1.7137363 8.536446 50-59
CTCCA 3979750 1.7134626 7.801308 150-159
GGTCA 4931445 1.7062911 7.4244914 130-139
TGCAG 4901310 1.6958642 13.010269 150-159
TTGGT 5443545 1.6947566 28.86294 25-29
TGACT 4133405 1.6896799 11.5418005 190-199
CAAAA 2144185 1.6888034 22.433006 70-79
GTCAC 4365435 1.6849089 8.385789 140-149
CACCA 3322445 1.68375 12.694073 230-239
TCAGA 3498740 1.6834842 10.372418 130-139
TCCCA 3907540 1.6823729 10.9036 240-247
ATAGT 3297930 1.6806852 7.2237587 220-229
AGTCT 4091545 1.672568 12.392697 210-219
CAGAC 3618280 1.6438115 19.20105 60-69
CTGTG 5560025 1.6343862 5.3829665 210-219
GAGAA 3205610 1.6275675 10.396012 160-169
AACTG 3380760 1.626716 10.329216 160-169
AATAG 2699920 1.6195593 9.111768 240-247
TGGTT 5179920 1.6126817 17.391962 10-14
ATAGA 2663040 1.5974365 9.946389 240-247
TGTGC 5426435 1.595117 7.8414183 220-229
GCTCC 5143870 1.59254 6.2692685 150-159
TTCAG 3880760 1.586402 9.076087 150-159
TGCTC 4831220 1.5841808 5.3873067 150-159
TCTGG 5354245 1.5738965 11.489289 180-189
ACCAT 2932035 1.5737529 13.136198 45-49
GCTGA 4524195 1.5653816 25.344498 30-34
TTGTA 3614195 1.5647885 8.583357 210-219
CTGGT 5302295 1.5586258 6.7183905 160-169
ACTGA 3237765 1.5579112 8.311053 180-189
GTCTG 5293070 1.5559139 8.525508 210-219
GGCCG 6608145 1.5523595 12.807822 120-129
GGACG 5294140 1.5504464 6.9996123 170-179
TAGTT 3559060 1.5409175 9.328489 220-229
GCTCA 3990005 1.5400058 10.811932 140-149
GTGCT 5226195 1.536256 7.4769135 220-229
GAACC 3358630 1.5258504 11.876785 70-79
CCCAC 3752915 1.5255978 10.306453 240-247
CATGT 3721485 1.5212926 9.378655 70-79
CCACC 3690635 1.5002804 9.113582 230-239
CCTCG 4744540 1.4689075 9.33804 130-139
CAAAG 2591400 1.4676847 26.483618 70-79
ACCGT 3789220 1.4625096 22.593735 45-49
GTGGA 4713545 1.462031 25.781591 25-29
GCTCT 4384800 1.4377975 9.474233 130-139
CGACT 3716955 1.4346178 8.295818 190-199
TCGAC 3653175 1.4100009 8.95866 190-199
CCTCA 3258380 1.4028801 7.4449472 130-139
CTCTT 3605315 1.396718 7.937209 200-209
CACTG 3605320 1.3915305 6.9861536 230-239
GTCCA 3602805 1.3905598 19.561878 70-79
TGATA 2724440 1.3884244 7.299088 180-189
TGGAC 4006720 1.3863343 10.063255 170-179
CCATG 3588325 1.384971 9.533249 70-79
GTAGG 4408510 1.3674161 5.2681265 160-169
GGGAG 5152205 1.3526467 6.3684525 150-159
GCGTA 3890820 1.3462324 9.897598 210-219
CCATC 3126515 1.3461064 10.674961 240-247
GGAGG 5121245 1.3445185 19.461485 30-34
GAGGG 5121125 1.3444871 7.808401 200-209
TATAC 2359500 1.3413279 6.5000315 110-119
ACTTC 2941475 1.3413178 6.6566095 220-229
AATTG 2623785 1.3371288 8.551446 170-179
AGATC 2769290 1.3324956 6.979987 130-139
CTCGA 3422990 1.3211571 8.0106735 190-199
TATGG 3603060 1.320377 6.0183544 190-199
GTGGT 4996535 1.3166698 12.919787 50-59
TGGTC 4476770 1.3159602 14.575879 50-59
CTGCT 4009560 1.3147545 5.360587 90-99
ACGGA 3212850 1.3084896 8.909215 200-209
TCCAC 3020240 1.3003502 9.266365 240-247
CTTGA 3176585 1.2985449 8.113996 200-209
TTGGC 4399830 1.2933432 9.332758 60-69
CTGAT 3155180 1.2897948 6.9873757 180-189
AGCCG 3897895 1.27339 8.982563 120-129
GTGTT 4083290 1.2712642 7.2315755 160-169
TGCCC 4082850 1.2640487 8.322424 60-69
ACGTG 3635795 1.2579932 8.963839 170-179
ATCGG 3630435 1.2561387 7.813853 190-199
ATAAA 1500105 1.251371 5.50653 7
GAATT 2453010 1.2500986 9.493285 170-179
ACTAT 2173215 1.2354287 6.2561755 230-239
GGAGT 3976845 1.2335237 6.5933514 210-219
ACTCG 3164295 1.2213099 8.213701 190-199
AAGTA 2033795 1.2199812 15.042455 70-79
GGCTC 4393040 1.2192577 7.9073186 130-139
GATCT 2980035 1.2181978 5.5404897 130-139
ATAAC 1794055 1.200471 7.069724 80-89
TTGAG 3259475 1.1944667 7.8130727 200-209
GCATA 2481210 1.1938806 7.763285 210-219
CCATA 2223010 1.1931878 11.886189 70-79
ATACC 2205490 1.1837841 8.598292 70-79
ACTGG 3407025 1.1788383 6.8432226 160-169
ATGGG 3794595 1.1769942 36.57996 15-19
GGAAT 2713540 1.1704789 7.977554 170-179
ACTGT 2840270 1.1610638 5.41089 230-239
AAAAG 1639295 1.1574546 15.408311 70-79
TTCCA 2534235 1.1556154 7.210251 230-239
TATCA 2026655 1.1521122 7.3820577 70-79
TCTGT 3299645 1.1459423 6.5007863 210-219
AGAGA 2244925 1.1398039 14.7371855 35-39
CAATA 1699285 1.1370568 6.6107717 100-109
AGTTC 2780025 1.1364365 5.443925 160-169
AAAGT 1894040 1.1361486 19.219297 70-79
AGCTT 2760060 1.128275 5.9373083 150-159
TCACG 2872940 1.1088568 8.959148 200-209
GAAGA 2183845 1.1087921 19.925825 35-39
AGAGC 2717270 1.1066558 6.7061834 140-149
TCTAC 2383320 1.0867981 6.990179 180-189
AGGTT 2953855 1.0824693 22.312666 10-14
GTCTA 2647990 1.0824624 7.209477 180-189
AACTT 1902615 1.0815982 6.0475793 200-209
ATCCA 2010490 1.079119 14.9301195 240-247
ATATC 1894095 1.0767546 7.883549 70-79
CTGAA 2225100 1.0706486 16.057688 35-39
CTGCC 3441610 1.065521 5.8885593 200-209
CTCGG 3825475 1.0617338 7.933879 130-139
CGGCT 3820910 1.060467 7.449198 130-139
TACTG 2560025 1.0465034 6.064289 180-189
GTCCG 3758175 1.0430553 5.0534124 130-139
TATGT 2403765 1.0407252 7.1136374 220-229
TCCCC 2991730 1.0332198 5.3689547 80-89
GAGCT 2985315 1.0329257 5.9884224 140-149
CATGG 2982185 1.0318427 5.340584 190-199
GCTTC 3143700 1.0308348 5.250761 150-159
GCGGT 4118300 1.0246565 5.668123 130-139
CGTGG 4103720 1.0210291 8.90627 50-59
CTGCG 3663500 1.016779 6.45638 210-219
TAGAT 1991605 1.0149583 7.5532923 240-247
CTTCT 2580585 0.9997322 5.4244213 210-219
TATAA 1396245 0.98952127 6.3716545 240-247
ACGTC 2562650 0.98909545 13.467938 70-79
CTACC 2289070 0.98554844 7.7962 230-239
ACAAT 1457615 0.97534615 7.6475673 100-109
TGCGT 3314345 0.9742618 7.798095 210-219
GGTGG 4357405 0.9718925 25.599808 25-29
CCATT 2124310 0.9686891 6.414937 50-59
GGGGT 4321570 0.9638998 7.1892223 10-14
GAGGC 3289155 0.96326464 16.50248 30-34
TAGGC 2783630 0.9631422 5.9018097 160-169
CGAAC 2117690 0.9620822 12.491409 70-79
AAGAG 1893640 0.9614479 14.805924 35-39
CCCAA 1871355 0.94836605 9.318042 240-247
GAGTT 2587395 0.9481765 5.247666 160-169
AGCTG 2733600 0.9458316 5.1646414 30-34
TGAAG 2188025 0.9437994 10.660414 35-39
ACCCA 1839080 0.9320097 12.216214 240-247
CGGAG 3179810 0.93124187 6.21714 210-219
GGGCC 3900735 0.91634536 19.403585 15-19
TGCAT 2231680 0.91228056 5.9228845 210-219
CAGCG 2792355 0.91222495 5.8140297 160-169
TGTAA 1784225 0.9092737 6.3289275 230-239
ATCAA 1350800 0.903872 14.214873 70-79
ACTAA 1333150 0.8920617 11.63091 240-247
CGTCC 2875910 0.89038056 10.995312 70-79
TCGAA 1844320 0.8874291 16.437561 70-79
CATCA 1647430 0.8842486 5.4936457 230-239
TTCTG 2542595 0.88302445 5.382228 210-219
GACCG 2677315 0.8746429 20.735733 45-49
TGGGC 3503000 0.87156653 21.019014 15-19
CCCCC 2652485 0.86491954 5.130756 80-89
CCAAT 1597095 0.85723156 10.09908 240-247
GGTCC 3088590 0.85721666 14.720071 50-59
CGGTC 3041975 0.844279 5.3729696 130-139
CCAGC 2248985 0.8195734 5.4255166 80-89
TGAAC 1669095 0.80311626 6.0097356 190-199
CTTCC 2188855 0.8006357 5.0496707 230-239
GTATG 2180805 0.7991776 5.0139585 220-229
AGCGT 2294040 0.7937429 5.9211645 160-169
AAAGA 1109980 0.7837219 5.877706 8
CATAC 1436530 0.7710492 10.296291 70-79
TAACC 1436355 0.77095526 5.638648 80-89
AACCG 1677325 0.7620212 5.325088 190-199
TGAGA 1766570 0.76200575 5.394638 240-247
CCTTT 1931245 0.7481745 5.606394 50-59
AAAGG 1451290 0.73685586 8.784244 10-14
AATCC 1359660 0.72978973 8.942363 240-247
ATGAT 1427465 0.72746223 6.691205 240-247
AGCCA 1587325 0.72113353 5.527711 80-89
AAAGC 1261690 0.71458024 10.448426 70-79
GTAGC 2063065 0.7138251 5.427025 80-89
GTCGA 2059770 0.71268505 11.335874 70-79
CTAAT 1253365 0.7125126 8.94392 240-247
CATTG 1712705 0.7001306 5.8734055 50-59
CGATG 2007770 0.69469285 16.202124 15-19
AGGAT 1595110 0.6880469 6.9384537 10-14
AAGGT 1573085 0.6785465 9.506409 10-14
TAGGT 1846155 0.67654175 5.268603 10-14
GGAAG 1841830 0.67244893 7.074025 30-34
CCGTA 1694485 0.6540134 5.5012403 80-89
AGAAG 1276565 0.6481436 5.382767 9
GAGGT 2088695 0.64786404 6.558476 10-14
TATGA 1251255 0.6376624 5.5758734 240-247
CAATC 1177915 0.63223916 8.676383 240-247
ATAGG 1449215 0.62511533 5.2135897 10-14
CATCC 1390340 0.5986044 7.365796 240-247
TAAAA 704980 0.5880865 5.9678288 8
AATGG 1337885 0.57709354 6.5185065 10-14
AAGAA 807355 0.570048 5.2964807 8
AAATG 942145 0.5651499 5.134727 9
GGGTC 2232485 0.5554551 5.3619256 70-79
GAAAA 782765 0.5526857 5.9466496 8
AAGCA 968550 0.54855525 7.07719 70-79
GAAGG 1478325 0.5397339 5.41462 10-14
TTGCC 1566315 0.51360244 5.8177886 60-69
ACGAT 1065255 0.5125674 7.212087 10-14
AAGGG 1385830 0.50596416 6.0763636 10-14
TGATC 1217290 0.49761164 5.2094083 240-247
ATAAG 808465 0.48496136 5.6148577 9
GGTCG 1922070 0.47822198 5.461008 70-79
GATCC 1237975 0.47781605 5.203697 240-247
GAAGC 1124620 0.4580212 5.7062917 30-34
TACCG 1162290 0.4486043 5.446903 70-79
AAGCT 915430 0.4404763 9.510764 30-34
CGGTT 1487755 0.4373301 7.728057 10-14
TAAGG 1008065 0.43482637 5.7738748 10-14
TAAAG 663375 0.39792848 5.643754 9
Produced by FastQC (version 0.10.1)