FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_13_SL248342.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_13_SL248342.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10264399
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11626090 8.901947 61.856266 110-119
ATACA 10687340 7.7053485 37.47036 110-119
CACAG 15772180 7.6494718 41.843216 110-119
TCCCT 18079600 7.0770144 102.44408 30-34
CAGTA 12943780 6.6669846 39.232212 110-119
AGTAA 10186405 6.5617447 47.821026 110-119
GGAGA 16176230 6.2627993 94.61515 35-39
GTAAT 11013190 6.024349 39.620483 110-119
GGTGA 18051130 5.9346366 102.32109 45-49
CCCTG 17670835 5.8192286 66.64604 30-34
AATAC 7765620 5.598849 39.50521 110-119
AGACG 12827360 5.5584373 102.645096 40-44
ACAGT 10500435 5.408485 35.756245 110-119
GTAGT 13790635 5.3892326 32.4773 80-89
GTGAC 13986135 5.1464972 85.56258 45-49
CCCCA 11290790 4.900702 102.41159 60-69
TAGTA 8935400 4.8877726 42.29869 80-89
GAGAC 11242370 4.871619 142.54073 40-44
CTCAG 11553940 4.7584825 34.83633 140-149
ACTCC 9974875 4.598012 115.67139 30-34
GGCCC 16291010 4.5133953 58.121983 60-69
ACCAG 9151140 4.438282 89.566086 45-49
GCACA 9143925 4.4347835 34.31755 110-119
TGACC 10738945 4.422827 146.95145 45-49
GTGTC 14125835 4.4139442 23.63396 120-129
AGTAG 9553570 4.3965273 33.901955 80-89
CGTGT 13915030 4.348073 23.067276 120-129
CACTC 9169255 4.226654 94.45731 25-29
CTCCC 11155545 4.1117187 66.21091 30-34
CCCAG 10522030 4.080461 87.40818 60-69
ATGCA 7675840 3.9536138 92.760155 25-29
GACCA 8151130 3.9532795 103.60321 45-49
CCCTT 9988035 3.9096808 42.30595 20-24
TGAGG 11606575 3.8158722 69.03988 35-39
TACAC 6578495 3.7924445 27.051146 110-119
AGGAG 9743815 3.7724214 94.84914 35-39
ACGGT 10194975 3.7514591 96.863014 40-44
GACGG 12088970 3.7424016 108.45841 40-44
ATGGT 9490535 3.7087994 26.004543 190-199
TGTCC 10567050 3.6956487 20.475233 120-129
AGATA 5722300 3.686116 19.437744 150-159
CTGAG 9970420 3.6688294 104.4791 35-39
CAGGG 11584680 3.5862877 33.547794 50-59
CCACT 7758985 3.576577 27.971943 240-247
GAGGA 9221655 3.5702617 124.99356 35-39
GCCCC 11346945 3.5185087 73.11 60-69
CTTGG 11234265 3.5104063 24.12912 20-24
TGGTG 12555840 3.5053723 19.163029 25-29
AGGCT 9448085 3.476625 29.872494 30-34
TGCAC 8429645 3.4717433 110.91083 25-29
CCGTG 11736060 3.4530778 23.172733 120-129
CAGAT 6462865 3.3288438 17.900974 150-159
GGATG 10118405 3.3266096 66.97584 20-24
CCTGA 8071335 3.3241737 81.20504 30-34
TCTTG 8935605 3.3188646 18.013674 170-179
CTCTC 8456850 3.3103194 13.644702 100-109
GGGTT 11835635 3.3043034 39.54962 50-59
TCAGG 8911950 3.2793427 20.811481 140-149
CCAGG 9271830 3.212557 38.18027 50-59
TCGCA 7751635 3.1925054 23.66132 100-109
CTTCA 6520885 3.192257 16.71441 200-209
GCACT 7747830 3.1909387 85.64528 25-29
TCTCA 6439495 3.152413 21.680977 130-139
TGTCT 8427410 3.1301105 19.718182 180-189
ACAGA 5137830 3.116373 14.497964 140-149
CCTGG 10576775 3.1119835 27.86206 50-59
CGCAC 7994765 3.1003835 24.169733 100-109
GCCCT 9091040 2.9937937 22.596996 20-24
CGGAT 8104380 2.98218 76.16656 20-24
GGTTG 10565250 2.949634 70.31595 15-19
TGGCC 9863775 2.9021988 42.450275 60-69
TTCCC 7390950 2.8930871 47.01003 50-59
GATGG 8792950 2.890842 23.224611 15-19
GACTC 6967515 2.8695662 19.58402 190-199
TCAAA 3972675 2.8642156 56.7184 70-79
GATGC 7775155 2.8610344 92.19812 25-29
TCTCT 6857615 2.8507755 15.016432 180-189
GGTTC 9099905 2.843476 44.754993 50-59
CGGTG 10701860 2.8133192 67.913536 40-44
AGGGT 8536675 2.8065872 47.337418 50-59
ACCAC 5117960 2.7781832 18.834166 230-239
TCACA 4802970 2.7688699 11.820682 140-149
GCCGT 9390130 2.7628396 21.874907 120-129
TTCTT 6221345 2.7466466 20.649557 170-179
TCCAT 5608280 2.745497 21.17195 70-79
GTTGG 9743625 2.7202504 50.6425 15-19
CGGCC 9801810 2.715574 12.475356 120-129
AGTCA 5186480 2.671413 29.566883 70-79
AGATG 5746895 2.6447058 17.130466 180-189
GGCTG 9970195 2.620978 15.424097 30-34
TGGAG 7955590 2.6155446 20.395927 25-29
GTTCC 7422130 2.5957656 50.76355 50-59
CCAGT 6263655 2.5796819 33.699852 60-69
AGGGA 6557160 2.538674 9.039047 150-159
TCCTC 6479230 2.5362067 16.896027 130-139
TGTTC 6670850 2.4776888 12.796427 140-149
ACTCT 5020970 2.4579833 13.9846735 200-209
GCGGA 7888830 2.4421577 83.284584 20-24
GAGAT 5272900 2.4265745 16.652798 180-189
ACAGC 4992155 2.4211838 11.16703 120-129
AATAA 2682915 2.4191322 10.646511 110-119
GGCGG 10712560 2.369191 39.977715 20-24
TGTAG 6042550 2.361364 8.019974 210-219
GTTCT 6342685 2.3558016 17.248499 160-169
CTGGA 6349050 2.336269 14.520733 180-189
CAGCT 5644480 2.324675 12.171106 150-159
CACGG 6675240 2.3128755 9.689359 120-129
TTGGG 8208525 2.2916775 76.159676 15-19
CATTT 4398535 2.2867987 17.715021 150-159
TCATT 4370145 2.272039 16.492945 150-159
TACCA 3935405 2.2687263 15.955884 230-239
TTCAT 4339675 2.2561975 15.883714 140-149
CAGAG 5206545 2.2561347 9.246369 140-149
CTGGC 7643655 2.2489774 32.7781 60-69
GTGAA 4773065 2.1965516 17.919292 190-199
TTGTC 5903500 2.1926796 13.148468 170-179
TCAGC 5294735 2.180633 14.127229 140-149
GATAC 4232665 2.1801293 14.467842 150-159
ATGTA 3954245 2.1630201 9.490011 70-79
TTCAC 4388295 2.148261 18.468513 200-209
ATTTG 4612125 2.142381 15.576562 150-159
CTACT 4365905 2.1373005 7.708263 230-239
CTCGC 6457750 2.1266181 16.233974 100-109
ACTAC 3678950 2.1208823 12.4946165 230-239
GAATC 4060470 2.0914364 14.787549 190-199
GCTGT 6629050 2.0714 9.971358 140-149
GAACT 4014925 2.0679774 10.830419 160-169
GTCCT 5901945 2.0641062 14.325725 130-139
CTCTG 5901570 2.0639753 14.4046545 180-189
TCTCG 5897580 2.0625799 15.816141 100-109
ATCTC 4204780 2.0584226 8.041275 100-109
CACTA 3561720 2.0533001 14.978801 240-247
TACAG 3984800 2.052461 13.51985 160-169
CCAGA 4219895 2.0466394 31.698591 60-69
TCTGC 5850665 2.0461721 12.034836 210-219
TGAAT 3737355 2.0443788 14.272346 190-199
TTGGA 5230785 2.0441349 15.380725 170-179
AGCTC 4955070 2.0407422 12.152686 140-149
ACACA 2990005 2.0298593 13.666791 110-119
GTCTC 5802990 2.0294986 11.72444 180-189
CAGGC 5846930 2.025878 15.2425165 140-149
GTTCA 4629325 2.0248117 14.823538 140-149
TGGGG 8570790 2.013058 49.83964 15-19
AATCG 3904425 2.011062 13.572943 190-199
TTTGC 5387410 2.0009933 11.894179 150-159
TAGTC 4550310 1.9902514 24.075653 70-79
ACGGC 5741105 1.9892111 14.355845 120-129
GATAT 3626370 1.9836686 8.147531 180-189
TTGCA 4533700 1.9829866 14.051797 150-159
ATATA 2585690 1.9798295 10.437828 110-119
TGGAA 4285875 1.9723481 8.794687 170-179
ACACG 4057875 1.9680601 13.651426 120-129
GTCAA 3819355 1.9672446 37.76773 70-79
AATAT 2562810 1.9623103 13.139847 110-119
AACCA 2871360 1.9493134 14.653143 70-79
GACGT 5290130 1.9466165 10.081094 60-69
CCTTG 5540630 1.9377427 27.478834 20-24
CAGCC 4917125 1.9068693 10.131564 120-129
ATATG 3485140 1.9064143 8.38961 180-189
CCTTC 4833615 1.8920531 13.70863 200-209
GAGTC 5134250 1.8892572 12.219267 210-219
TCGGC 6366055 1.8730717 7.5173864 130-139
CATAG 3634290 1.8719229 8.503065 210-219
TACTA 3044700 1.8640856 10.368231 240-247
CTGTT 4854760 1.8031561 12.468399 140-149
GGGGC 8109920 1.7935909 45.282604 15-19
AGAAC 2945240 1.7864481 11.49673 160-169
GTTGT 5378595 1.7848846 6.9924073 210-219
GTCCC 5358540 1.7646345 23.71371 50-59
AGTAT 3223870 1.7634962 11.909441 220-229
GGGCG 7909875 1.7493489 55.734665 20-24
CGTAG 4749710 1.7477574 9.638 210-219
GCAGA 4001360 1.7338963 14.219186 150-159
GGTCA 4699775 1.7293828 7.5722594 130-139
ATTGT 3723020 1.7293823 8.670613 50-59
CTCCA 3726365 1.7177029 7.925964 150-159
TGCAG 4645800 1.7095215 13.202383 150-159
TTGGT 5137375 1.7048359 29.093111 25-29
GTCAC 4137400 1.7039852 8.631749 140-149
TCAGA 3304720 1.7021704 10.547777 130-139
TGACT 3874610 1.6947083 11.901135 190-199
CAAAA 1995935 1.6946126 22.782793 70-79
ATAGT 3062125 1.6750197 7.5578656 220-229
CAGAC 3434355 1.6656544 19.375483 60-69
AGTCT 3801100 1.6625559 12.930479 210-219
TCCCA 3589625 1.6546713 11.5309305 240-247
AACTG 3211795 1.6543071 10.662019 160-169
GAGAA 3052090 1.6540278 10.680067 160-169
CACCA 3025830 1.642512 13.209908 230-239
TGGTT 4924090 1.6340574 17.509127 10-14
CTGTG 5204805 1.6263616 5.5448637 210-219
AATAG 2507125 1.6150068 9.544739 240-247
TTCAG 3689835 1.6138899 9.309781 150-159
GCTCC 4830510 1.5907476 6.331627 150-159
ACCAT 2747195 1.5837336 13.266385 45-49
TGCTC 4523300 1.5819484 5.486717 150-159
ATAGA 2454710 1.5812428 10.43575 240-247
GCTGA 4290540 1.578796 25.37786 30-34
TGTGC 5043415 1.5759315 8.167494 220-229
TCTGG 5014725 1.5669669 11.699451 180-189
CTGGT 5008585 1.5650482 6.880973 160-169
ACTGA 3034720 1.5631008 8.556304 180-189
GCTCA 3793635 1.5624058 10.973412 140-149
GGCCG 6299125 1.559234 12.808602 120-129
TTGTA 3341585 1.5522016 9.010932 210-219
GTCTG 4940110 1.5436516 8.828844 210-219
GGACG 4984180 1.5429608 7.1300097 170-179
CATGT 3527140 1.5427291 9.479907 70-79
GAACC 3178325 1.5414804 12.068583 70-79
TAGTT 3289135 1.527838 9.784623 220-229
GTGCT 4847785 1.5148025 7.783225 220-229
ACCGT 3613210 1.488098 22.70884 45-49
CCTCG 4509445 1.4850171 9.411998 130-139
CCCAC 3415270 1.4823782 10.604748 240-247
CAAAG 2443300 1.4819943 26.712404 70-79
GTGGA 4473130 1.4706227 25.817617 25-29
CCACC 3368350 1.4620129 9.417895 230-239
AAATA 1621265 1.4618632 5.007936 9
GCTCT 4140625 1.4481142 9.623821 130-139
CGACT 3488520 1.4367447 8.569655 190-199
CCTCA 3066585 1.4135711 7.5416393 130-139
TCGAC 3429620 1.4124866 9.247043 190-199
GTCCA 3414760 1.4063665 19.726463 70-79
CCATG 3409280 1.4041097 9.608578 70-79
TGGAC 3782855 1.3919823 10.26862 170-179
CTCTT 3347030 1.3913921 8.235534 200-209
TGATA 2543600 1.3913801 7.5137773 180-189
AAAAA 1300210 1.380599 8.21448 8
CACTG 3318035 1.3665305 7.285882 230-239
GTAGG 4130355 1.3579291 5.318685 160-169
TATAC 2215430 1.3563735 6.597958 110-119
AGATC 2630695 1.3549986 7.1323133 130-139
AATTG 2472775 1.3526381 8.791061 170-179
GGGAG 4854760 1.3427792 6.4359145 150-159
ACTTC 2712360 1.3278182 6.9857388 220-229
CCATC 2874960 1.3252398 11.342993 240-247
GGAGG 4785630 1.3236585 19.365765 30-34
CTCGA 3213380 1.3234283 8.273782 190-199
TATGG 3379230 1.320567 6.1796875 190-199
GCGTA 3588330 1.3204029 10.2304125 210-219
TGGTC 4222610 1.3194523 14.635042 50-59
CTGCT 3767365 1.3175727 5.4164147 90-99
GAGGG 4735220 1.3097155 7.9597073 200-209
GTGGT 4666500 1.3028055 12.931993 50-59
CTGAT 2967675 1.2980257 7.204788 180-189
TTGGC 4152865 1.2976588 9.362457 60-69
AGCCG 3720705 1.2891712 9.059302 120-129
ACGGA 2972125 1.287901 9.152502 200-209
CTTGA 2922700 1.2783543 8.37459 200-209
TCCAC 2761510 1.2729439 9.698699 240-247
ACGTG 3454060 1.2709953 9.202269 170-179
GAATT 2308065 1.2625397 9.7518835 170-179
GTGTT 3794075 1.2590623 7.357489 160-169
ATCGG 3410660 1.2550254 8.009583 190-199
TGCCC 3805515 1.2532039 8.226564 60-69
GATCT 2831435 1.238436 5.6473484 130-139
AAGTA 1915860 1.2341335 15.204303 70-79
ACTAT 2010680 1.2310177 6.5372515 230-239
ATAAA 1362075 1.2281566 5.745016 7
GGCTC 4148105 1.2204887 7.9928427 130-139
ACTCG 2954435 1.2167821 8.468652 190-199
GGAGT 3692530 1.2139864 6.7444725 210-219
CCATA 2092860 1.2065153 12.036444 70-79
ATAAC 1672170 1.2055997 7.1561255 80-89
ATGGG 3607245 1.1859473 36.530003 15-19
TTGAG 3027910 1.1832749 8.082473 200-209
ATACC 2052190 1.1830695 8.72253 70-79
ACTGG 3210530 1.1813833 6.993611 160-169
GCATA 2287740 1.178352 8.112765 210-219
GGAAT 2535480 1.1668211 8.158295 170-179
TATCA 1897595 1.1617825 7.509778 70-79
AGTTC 2633780 1.151984 5.5748196 160-169
AAAGT 1786085 1.1505367 19.422611 70-79
ACTGT 2616060 1.1442336 5.668912 230-239
AGCTT 2614795 1.1436802 6.1081476 150-159
AAAAG 1499235 1.1372854 15.565389 70-79
CAATA 1575390 1.1358231 6.7616143 100-109
AGAGA 2094785 1.1352328 14.83046 35-39
TTCCA 2312530 1.132084 7.54058 230-239
TCTGT 3045790 1.1312681 6.7029095 210-219
AGAGC 2585400 1.1203228 6.8444614 140-149
GAAGA 2066955 1.1201508 19.99995 35-39
ATATC 1794090 1.0984128 8.041182 70-79
AGGTT 2809835 1.0980535 22.488626 10-14
GTCTA 2509505 1.0976276 7.4417305 180-189
TCTAC 2234400 1.093836 7.2394075 180-189
TCACG 2645740 1.0896463 9.199836 200-209
AACTT 1773045 1.085528 6.3553896 200-209
CTGAA 2097605 1.0804186 16.163633 35-39
CTCGG 3626550 1.0670326 7.969751 130-139
ATCCA 1845955 1.0641768 15.918865 240-247
CGGCT 3613015 1.0630503 7.508164 130-139
GTCCG 3576740 1.0523771 5.125478 130-139
CTGCC 3181525 1.0477161 6.0332103 200-209
TACTG 2395190 1.0476276 6.232931 180-189
GAGCT 2839565 1.0448787 6.1061115 140-149
GCTTC 2952655 1.0326415 5.38706 150-159
CATGG 2803225 1.0315067 5.4818707 190-199
GCGGT 3911275 1.0282012 5.7527866 130-139
CGTGG 3874005 1.0184035 8.873629 50-59
TCCCC 2744465 1.0115569 5.3764734 80-89
TATGT 2177375 1.0114137 7.3651404 220-229
ACGTC 2446665 1.0076573 13.590776 70-79
CTGCG 3386650 0.99644727 6.6415653 210-219
TAGAT 1821105 0.99616665 7.9377317 240-247
CTTCT 2377490 0.98834515 5.6317186 210-219
ACAAT 1362455 0.9823014 7.848149 100-109
AAAAT 1089310 0.9822096 5.297601 8
CGAAC 2021195 0.9802751 12.701815 70-79
TATAA 1277350 0.9780505 6.649495 240-247
CCATT 1986885 0.9726666 6.515872 50-59
AAGAG 1787105 0.96849084 14.90846 35-39
TAGGC 2630260 0.96786034 5.9709225 160-169
GAGGC 3108900 0.9624272 16.473087 30-34
CTACC 2073115 0.9556218 8.049861 230-239
AGCTG 2587525 0.9521352 5.207145 30-34
GGTGG 4049800 0.9511938 25.435244 25-29
TGCGT 3044030 0.95117754 8.070339 210-219
GAGTT 2433680 0.9510561 5.3491883 160-169
TGAAG 2061690 0.94878423 10.696745 35-39
GGGGT 4005685 0.9408324 7.126705 10-14
CCCAA 1712500 0.9295968 9.783723 240-247
CAGCG 2635575 0.9131892 5.870904 160-169
GGGCC 3689120 0.91317475 19.219307 15-19
ATCAA 1265375 0.9123089 14.456752 70-79
ACCCA 1675095 0.9092922 12.774123 240-247
TCGAA 1763805 0.9084874 16.73723 70-79
CGGAG 2927375 0.9062321 6.331777 210-219
TGCAT 2052525 0.89775 6.172992 210-219
CGTCC 2721635 0.8962686 11.053783 70-79
TGTAA 1626605 0.8897727 6.648494 230-239
GACCG 2547210 0.882572 20.715094 45-49
TGGGC 3318745 0.8724361 20.882816 15-19
CATCA 1503925 0.8669995 5.70863 230-239
TTCTG 2331290 0.8658883 5.5501976 210-219
ACTAA 1188460 0.8568548 12.191417 240-247
GGTCC 2906720 0.85523844 14.673423 50-59
CGGTC 2894910 0.8517636 5.4683986 130-139
CCAAT 1462015 0.8428387 10.652905 240-247
CCAGC 2103910 0.81589985 5.4711595 80-89
AGCGT 2167005 0.7973959 6.038294 160-169
TGAAC 1540275 0.79335326 6.13551 190-199
TAACC 1345555 0.7757006 5.681181 80-89
GTATG 1974935 0.7717835 5.1084075 220-229
CTTCC 1969580 0.7709654 5.2833476 230-239
CATAC 1328735 0.766004 10.454164 70-79
AACCG 1550085 0.7517877 5.4317017 190-199
TGAGA 1624250 0.74747545 5.6909947 240-247
AAAGA 984965 0.7471718 5.963444 8
CCTTT 1788695 0.7435775 5.6176496 50-59
AAAGG 1359495 0.7367549 8.768044 10-14
AGCCA 1498795 0.7269122 5.593921 80-89
GTCGA 1962905 0.7222929 11.466219 70-79
AAAGC 1185170 0.7188699 10.553853 70-79
AATCC 1242420 0.7162441 9.422509 240-247
ATGAT 1302845 0.7126721 7.1239777 240-247
GTAGC 1936390 0.71253604 5.411544 80-89
CATTG 1607210 0.7029745 5.94458 50-59
CGATG 1905470 0.70115846 16.21486 15-19
TGTTA 1482990 0.6888645 5.1546507 230-239
CTAAT 1122450 0.6872082 9.39064 240-247
AAGGT 1487025 0.6843248 9.560244 10-14
AGGAT 1484910 0.6833516 6.970004 10-14
GGAAG 1724410 0.66762364 7.07811 30-34
TAGGT 1707865 0.6674154 5.3071985 10-14
CCGTA 1596760 0.65762454 5.57479 80-89
CCCAT 1405470 0.6478646 5.049418 240-247
AGAAG 1182885 0.6410442 5.514339 9
GAGGT 1946835 0.6400574 6.539969 10-14
TATGA 1142260 0.6248301 5.924233 240-247
ATAGG 1350575 0.621531 5.2583513 10-14
CAATC 1074105 0.61921203 9.148264 240-247
CATCC 1264645 0.58294994 7.8009987 240-247
AATGG 1239290 0.57031786 6.565711 10-14
AAATG 865450 0.5574942 5.2382555 9
TAAAA 617800 0.5570582 6.0356407 8
AAGCA 905545 0.5492623 7.1498013 70-79
GGGTC 2072550 0.54483473 5.3938265 70-79
AAGAA 711495 0.5397238 5.344364 8
GAAGG 1371815 0.53111273 5.45072 10-14
ACGAT 1002780 0.51650435 7.254901 10-14
GAAAA 671515 0.5093959 6.088539 8
TTGCC 1452750 0.508075 5.8067183 60-69
AAGGG 1292540 0.5004205 6.089322 10-14
TGATC 1114435 0.48744062 5.5009403 240-247
ATAAG 741945 0.47793642 5.6561074 9
GGTCG 1814055 0.47688118 5.494714 70-79
GATCC 1133500 0.46683118 5.5001574 240-247
GAAGC 1046945 0.45366922 5.728672 30-34
TACCG 1091015 0.44933382 5.508134 70-79
AAGCT 851020 0.43833694 9.632194 30-34
TAAGG 946355 0.43551004 5.7848177 10-14
CGGTT 1390935 0.4346298 7.737531 10-14
TAAAG 613535 0.39521894 5.625873 9