FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_9_SL248355.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_9_SL248355.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11745248
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13318910 8.87415 61.988968 110-119
ATACA 12189175 7.6716623 38.070312 110-119
CACAG 17798370 7.575899 42.33138 110-119
TCCCT 21038050 7.2476754 106.965 30-34
CAGTA 15120420 6.8133507 39.47262 110-119
AGTAA 11625985 6.5270205 48.19468 110-119
GGAGA 18476480 6.25768 94.20372 35-39
CCCTG 20931335 6.0759926 69.636185 30-34
GTAAT 12661195 6.039689 40.022335 110-119
GGTGA 20600305 5.928194 102.035904 45-49
AATAC 8846525 5.567855 40.06273 110-119
AGACG 14173700 5.3815484 101.776855 40-44
GTAGT 15567510 5.3166842 29.05794 80-89
ACAGT 11757845 5.2981544 35.813488 110-119
GTGAC 15842110 5.110833 84.63674 45-49
GAGAC 12848335 4.8783264 141.2161 40-44
CCCCA 12642930 4.8422065 101.66422 60-69
ACTCC 11852660 4.8056746 121.73334 30-34
CTCAG 13147965 4.7551823 35.344547 140-149
ACCAG 10951270 4.6614223 93.69704 45-49
TAGTA 9627490 4.5925403 37.19672 80-89
GCACA 10539670 4.4862237 34.367195 110-119
GGCCC 18215215 4.4553485 58.909237 60-69
CACTC 10897705 4.418487 98.97202 25-29
TGACC 12168085 4.4007926 144.93102 45-49
GTGTC 15968955 4.377336 23.893208 120-129
CGTGT 15859165 4.34724 23.343554 120-129
AGTAG 10730000 4.3128734 30.04677 80-89
CTCCC 12965975 4.219446 69.59364 30-34
ATGCA 9170675 4.1323605 96.80953 25-29
GACCA 9615840 4.092994 106.45479 45-49
CCCAG 11890055 4.0620894 86.777336 60-69
TGAGG 13295370 3.8260372 68.9835 35-39
AGGAG 11198415 3.7927184 94.44185 35-39
TACAC 7464650 3.7708244 27.656755 110-119
ACGGT 11632110 3.7526424 96.24492 40-44
CAGGG 13641475 3.7082338 35.47733 50-59
GACGG 13639085 3.7075841 107.48237 40-44
CTGAG 11463150 3.698134 105.11309 35-39
ATGGT 10822075 3.6960022 26.216259 190-199
CCACT 9019825 3.6570983 28.70119 240-247
AGATA 6489320 3.6432116 19.666643 150-159
CCCTT 10568990 3.6410506 35.81522 20-24
TGTCC 11842135 3.6390944 20.830544 120-129
TGCAC 10034415 3.6291149 115.916916 25-29
GAGGA 10552495 3.573956 123.97424 35-39
GCCCC 12695395 3.4811563 72.78782 60-69
GGGTT 14161310 3.462636 42.08009 50-59
CCTGA 9570245 3.4612396 84.51778 30-34
CAGAT 7598645 3.4239943 18.21762 150-159
TGGTG 13905115 3.399993 18.137716 190-199
GGATG 11792415 3.3935285 69.80741 20-24
AGGCT 10427460 3.3640094 24.98483 30-34
CCAGG 11013910 3.3564272 40.28269 50-59
CCGTG 12953415 3.3540895 23.392553 120-129
GCACT 9249115 3.3450975 89.56021 25-29
CTTGG 12135705 3.3265831 20.343239 20-24
CTCTC 9617760 3.3133488 13.7285 130-139
TCTTG 10170840 3.3087432 18.176126 170-179
CTTCA 7631705 3.2756922 17.245848 200-209
TCAGG 10124280 3.2662003 21.093088 140-149
CCTGG 12421825 3.216442 29.33875 50-59
TCGCA 8859885 3.204326 24.191603 100-109
TCTCA 7391920 3.1727712 22.24413 130-139
TGTCT 9632290 3.133544 19.886707 180-189
CGCAC 9096705 3.1077764 24.688011 100-109
CGGAT 9609465 3.1001155 79.42896 20-24
TTCCC 8882800 3.0601528 49.86394 50-59
GGTTG 12466485 3.048228 73.72383 15-19
ACAGA 5721310 3.034155 14.309713 140-149
GATGC 9273455 2.9917152 96.634895 25-29
TGGCC 11467025 2.9692113 43.440544 60-69
GGTTC 10814300 2.9643657 47.623234 50-59
AGGGT 10198900 2.934959 49.78603 50-59
TCAAA 4587785 2.887475 58.617714 70-79
GCCCT 9883605 2.8690336 19.20694 20-24
GTTGG 11640270 2.8462071 52.938927 15-19
TCTCT 7795040 2.8428538 15.331962 180-189
ACCAC 5946220 2.837428 20.160059 230-239
GACTC 7801445 2.8215234 19.865732 190-199
CGGTG 12192175 2.8160582 67.68785 40-44
AGTCA 6246320 2.8146288 31.948463 70-79
GCCGT 10732245 2.7789514 22.137796 120-129
GATGG 9644115 2.7753077 19.770527 15-19
CCAGT 7663100 2.7714884 36.52871 60-69
TTCTT 7140395 2.7567754 20.77862 170-179
CGGCC 11081905 2.7105772 12.622914 120-129
GTTCC 8819880 2.7103539 54.05267 50-59
TCACA 5244495 2.6492963 11.6027355 140-149
AGATG 6584525 2.646619 17.488031 180-189
TCCAT 6116465 2.6253188 18.188164 70-79
GCGGA 9417185 2.559923 87.00228 20-24
GGCTG 10998140 2.540269 12.97278 30-34
TCCTC 7364780 2.53719 17.11149 130-139
AGGGA 7392875 2.5038447 9.11747 150-159
TGGAG 8697175 2.5028048 17.062798 25-29
TGTTC 7670085 2.495206 13.0075035 140-149
ACAGC 5759660 2.4516065 11.211724 120-129
GGCGG 12585730 2.449435 41.989628 20-24
ACTCT 5642830 2.4220243 13.823928 200-209
GAGAT 6001660 2.4123392 16.947325 180-189
TTGGG 9821010 2.401373 79.37903 15-19
GTTCT 7291070 2.3719063 17.872122 160-169
CTGGC 9152235 2.3698318 34.72445 60-69
CAGCT 6540300 2.3654091 12.302005 150-159
AATAA 2997535 2.3505406 10.072225 110-119
TACCA 4637055 2.3424435 16.938087 230-239
CTGGA 7215995 2.3279567 14.763285 180-189
CACGG 7597220 2.31521 9.599115 120-129
CATTT 5060355 2.2993522 17.941288 150-159
TGTAG 6708310 2.2910514 8.134434 210-219
TCATT 5032760 2.2868135 16.678421 150-159
TTCAT 4948480 2.2485178 16.313774 140-149
TCAGC 6173115 2.2326105 14.694018 140-149
CAGAG 5826425 2.212209 9.387986 140-149
GTGAA 5479230 2.2023509 18.260082 190-199
TTCAC 5087485 2.1836581 18.884966 200-209
TTGTC 6690035 2.1763794 13.566737 170-179
GATAC 4811820 2.1682348 14.788586 150-159
CTACT 5028895 2.15851 7.578936 230-239
ACTAC 4266585 2.1552978 12.663135 230-239
ATTTG 5309270 2.151936 15.845379 150-159
TGGGG 10271130 2.116161 52.07547 15-19
CACTA 4186790 2.1149888 15.653803 240-247
CTCGC 7284060 2.1144326 16.448538 100-109
TAGTC 5500980 2.1061614 26.402046 70-79
ATCTC 4864990 2.0881584 8.457184 100-109
TCTCG 6794420 2.0879288 16.429958 100-109
GAACT 4629910 2.0862648 10.877823 160-169
TCTGC 6779180 2.0832458 12.443774 210-219
ATGTA 4366150 2.0827568 7.9989676 70-79
GCTGT 7559130 2.0720732 10.176677 140-149
GTCCT 6732490 2.0688977 14.3730545 130-139
TTGGA 6046220 2.0649314 15.700841 170-179
GAATC 4582195 2.0647645 14.726257 190-199
CTCTG 6690145 2.055885 14.768055 180-189
AGCTC 5683405 2.0554986 12.5075035 140-149
TACAG 4560465 2.0549724 13.776639 160-169
GTCTC 6665135 2.0481994 12.008023 180-189
ATATA 3071285 2.046342 10.653393 110-119
GTCAA 4522940 2.0380638 40.71713 70-79
ACACA 3426980 2.037435 13.892793 110-119
CAGGC 6684855 2.0371718 15.538354 140-149
GTTCA 5312585 2.0340307 15.170487 140-149
TGAAT 4232430 2.018969 14.215404 190-199
AATAT 3022315 2.0137143 13.34544 110-119
ACACG 4703340 2.0019827 13.398121 120-129
GATAT 4194350 2.0008042 8.290417 180-189
TTGCA 5214255 1.996383 14.4851265 150-159
AATCG 4428890 1.995684 13.742655 190-199
AACCA 3324010 1.9762164 14.996073 70-79
TTTGC 6049520 1.9680094 12.299593 150-159
ACGGC 6449300 1.965388 14.3598995 120-129
TGGAA 4866160 1.9559304 9.283812 170-179
CCTTC 5605940 1.9312639 14.248824 200-209
CAGCC 5574575 1.9044843 10.191138 120-129
GAGTC 5898630 1.9029605 12.615755 210-219
GGGGC 9712065 1.8901622 47.445778 15-19
TCGGC 7292610 1.8883103 7.850958 130-139
ATATG 3952145 1.8852664 8.455284 180-189
CATAG 4161540 1.8752146 8.679181 210-219
TACTA 3499840 1.8716215 10.200373 230-239
CCAGA 4368195 1.8593277 27.38643 60-69
GGGCG 9445125 1.8382103 58.696896 20-24
GACGT 5639010 1.8192046 9.674006 170-179
TCAGA 3987530 1.7968047 10.518087 130-139
CTGTT 5509660 1.7923839 12.740013 140-149
AGTAT 3738165 1.7831931 12.144097 220-229
CTCCA 4370385 1.7719778 8.21894 150-159
AGAAC 3310140 1.7554506 11.403335 160-169
GTTGT 6042800 1.7535355 7.087019 210-219
CGTAG 5402230 1.7428167 9.909098 210-219
GCAGA 4585700 1.7411238 14.544028 150-159
ATTGT 4283330 1.7361054 7.770269 50-59
AAAAA 1877470 1.7326936 7.9968147 8
CCTTG 5626690 1.7290846 23.193462 20-24
TGCAG 5355835 1.7278492 13.609619 150-159
GTCAC 4771905 1.7258396 8.663786 130-139
GGTCA 5248040 1.6930735 7.5944104 130-139
AGTCT 4409575 1.6882949 13.385676 210-219
ATAGT 3517855 1.6780999 7.7176056 220-229
CACCA 3493320 1.6669487 13.848056 230-239
TGACT 4353740 1.6669174 11.943875 190-199
AACTG 3671345 1.6543298 10.755378 160-169
TGGTT 5686550 1.6501567 18.100817 10-14
TTCAG 4291155 1.6429554 9.259846 150-159
CAAAA 2201445 1.6306785 21.585447 70-79
GAGAA 3434550 1.6247334 10.451465 160-169
TCCCA 3990140 1.617807 11.085979 240-247
GTCCC 5556035 1.6128176 20.541348 50-59
GCTCC 5518105 1.6018071 6.514921 150-159
GTCTG 5836765 1.5999469 9.252716 210-219
CTGTG 5832495 1.5987763 5.5859327 210-219
TGTGC 5804805 1.5911863 8.427738 220-229
CAAAG 2999400 1.5906574 29.181782 70-79
TGCTC 5163860 1.586857 5.451876 150-159
TTGGT 5465295 1.5859517 24.454815 25-29
AATAG 2824865 1.5859259 9.7133045 240-247
TCTGG 5758940 1.5786139 12.01697 180-189
ACTGA 3490420 1.5728039 8.784525 180-189
GAACC 3682790 1.5675842 12.392155 70-79
TAGTT 3865800 1.5668736 10.214492 220-229
CTGGT 5707680 1.5645627 6.998821 160-169
GCTCA 4295405 1.5535052 11.208631 140-149
GGCCG 7114380 1.5522244 13.0069 120-129
TTGTA 3821105 1.5487579 9.186859 210-219
GTGCT 5638975 1.5457295 8.209702 220-229
ACCAT 3053150 1.5423219 11.869781 45-49
ATAGA 2737200 1.5367093 10.166704 240-247
GGACG 5565080 1.5127851 7.153904 170-179
CAGAC 3511145 1.4945233 16.250488 60-69
AGATC 3297725 1.4859746 7.2957487 130-139
CATGT 3855850 1.4762901 7.9672174 70-79
CCTCG 5081310 1.4750135 9.591172 130-139
GCTCT 4795945 1.4737962 10.025327 130-139
CCACC 3838875 1.4702781 9.863473 230-239
CCCAC 3818260 1.4623827 10.560076 240-247
GCTGA 4522430 1.4589839 21.445696 30-34
AAATA 1812630 1.4213879 5.2929654 9
CGACT 3912910 1.4151695 8.763599 190-199
TATAC 2638630 1.4110693 6.7346053 110-119
AATTG 2927000 1.3962481 9.2922735 170-179
TGATA 2914720 1.3903903 7.7357707 180-189
TCGAC 3836525 1.3875437 9.398067 190-199
CCTCA 3416560 1.3852482 7.5523033 130-139
TGGAC 4288480 1.3835093 10.364188 170-179
CACTG 3811045 1.3783283 7.3771887 230-239
CTCTT 3752815 1.3686529 8.144557 200-209
CTGCT 4449260 1.36726 5.7449946 90-99
GTGGA 4672945 1.3447434 21.693531 25-29
GGGAG 5534445 1.341992 6.502327 150-159
CCATG 3703520 1.3394401 8.092589 70-79
GATCT 3496805 1.3388225 5.723585 130-139
GCGTA 4130080 1.3324077 10.392736 210-219
ACCGT 3672530 1.328232 19.173447 45-49
CCATC 3274160 1.3275119 11.446056 240-247
GTAGG 4593640 1.3219216 5.306362 160-169
CTGAT 3449210 1.3205999 7.3838625 180-189
ACTTC 3075485 1.3200644 7.056227 220-229
GAGGG 5432235 1.317208 8.199678 200-209
CTCGA 3597415 1.3010654 8.400154 190-199
GAATT 2726265 1.3004929 10.320388 170-179
ACGGA 3416525 1.297205 9.416391 200-209
CTTGA 3374005 1.2918062 8.310485 200-209
TCCAC 3175920 1.2876805 9.907089 240-247
TATGG 3768555 1.2870533 6.1184363 180-189
ATCGG 3988710 1.2868003 8.136015 190-199
AGCCG 4196975 1.2790046 9.090197 120-129
GTCCA 3523275 1.2742515 16.860199 70-79
GGAAT 3136615 1.2607478 8.617603 170-179
GTGTT 4343240 1.2603471 7.5438666 160-169
ACGTG 3883385 1.2528213 9.2899885 170-179
AAAGT 2226560 1.2500277 21.472294 70-79
AAGTA 2223465 1.2482901 15.8448305 70-79
GGCTC 4810770 1.2456756 8.304234 130-139
GGAGT 4304220 1.2386346 7.061628 210-219
ACTAT 2308785 1.2346768 6.3578825 230-239
ATAAC 1959680 1.2333897 7.6586246 80-89
GGAGG 5054010 1.2254962 16.15341 30-34
TTGGC 4459275 1.2223558 8.314858 60-69
GTGGT 4989545 1.2200127 10.920418 50-59
TGGTC 4425660 1.2131414 12.3855295 50-59
TCACG 3343265 1.2091478 9.624692 200-209
ACTCG 3326510 1.203088 8.579439 190-199
GCATA 2644725 1.1917286 8.338443 210-219
ACTGG 3672585 1.1848149 7.1240907 160-169
TGCCC 4056165 1.1774322 6.8792477 60-69
ATAAA 1499470 1.1758212 5.7000537 7
TTGAG 3418410 1.1674703 8.068196 200-209
ATACC 2308325 1.1660678 7.7564254 70-79
AGCTT 3043650 1.1653229 6.069563 150-159
TTCCA 2712930 1.164448 7.8887043 230-239
AGAGC 3057730 1.1609757 6.989515 140-149
TATCA 2136750 1.1426772 7.0026846 70-79
CCATA 2248345 1.1357685 10.695301 70-79
AGTTC 2961895 1.1340214 5.5619655 160-169
GAAGA 2379405 1.1255912 18.476334 35-39
ATATC 2101985 1.1240858 7.5059915 70-79
ACTGT 2934890 1.123682 5.6836195 230-239
TCTGT 3449290 1.1221114 6.860432 210-219
AGGTT 3277725 1.1194228 23.23055 10-14
CAATA 1772880 1.115821 6.692997 100-109
GTCTA 2859340 1.0947561 7.318919 180-189
AAAAG 1655310 1.0937294 14.43069 70-79
CTGAA 2421220 1.0910162 14.740087 35-39
TCTAC 2540000 1.0902227 7.2459483 180-189
CGGCT 4181700 1.0827875 7.7611094 130-139
ATCCA 2139535 1.0808023 16.12051 240-247
CTCGG 4152720 1.0752835 8.263754 130-139
GCTTC 3465265 1.0648776 5.418849 150-159
ATGGG 3681965 1.059567 30.977201 15-19
GAGCT 3281505 1.0586483 6.2529182 140-149
TACTG 2753770 1.0543365 6.4269004 180-189
TATGT 2574685 1.043563 7.6559772 220-229
AACTT 1951090 1.0433911 6.275365 200-209
AGAGA 2201730 1.041541 13.253947 35-39
GTCCG 4005240 1.0370959 5.0343475 130-139
CTGCC 3564510 1.0347136 5.994175 200-209
CATGG 3174480 1.024121 5.4871554 190-199
CTTCT 2800585 1.0213743 5.797954 210-219
GCGGT 4414525 1.0196341 5.7462354 130-139
CTGCG 3904310 1.0109617 6.8609023 210-219
TAGAT 2111495 1.007233 7.879324 240-247
CTACC 2482915 1.0067008 8.652835 230-239
ATACG 2211905 0.9966975 5.229742 120-129
GGGGT 4786375 0.9861369 7.585187 10-14
CGAAC 2298110 0.97819334 13.0219755 70-79
AAAAT 1243535 0.97512764 5.348656 8
AAGAG 2053880 0.97159964 13.710962 35-39
TGCGT 3519250 0.96468043 8.322586 210-219
TAGGC 2987110 0.9636734 5.9848924 160-169
TATAA 1444605 0.96251434 6.493806 240-247
AGCTG 2976520 0.960257 5.08917 90-99
TCCCC 2947910 0.9593222 5.051181 80-89
CAGCG 3141125 0.95724016 6.2191243 160-169
ACAAT 1508370 0.9493428 7.6489654 100-109
CGTGG 4084275 0.9433555 7.5554295 50-59
CCATT 2196365 0.94272715 5.950601 50-59
ACCCA 1973515 0.94172543 13.402403 240-247
GAGTT 2723010 0.9299742 5.299205 160-169
ACGTC 2566455 0.92820144 11.589448 70-79
AGTTT 2282525 0.9251457 5.1575108 220-229
CGGAG 3396240 0.9232178 6.6268067 210-219
TGTAA 1929410 0.92037416 7.0294604 230-239
TGCAT 2351675 0.9003864 6.375441 210-219
TCGAA 1997995 0.9003085 16.540493 70-79
CCCAA 1880520 0.8973499 10.043584 240-247
TTCTG 2756470 0.8967255 5.774184 210-219
TGAAG 2226525 0.89494133 9.729154 35-39
ACTAA 1416415 0.89146787 12.809221 240-247
TGAAC 1950755 0.8790219 6.575078 190-199
GAGGC 3169785 0.8616592 13.710161 30-34
ATCAA 1359995 0.85595804 13.152747 70-79
GGTGG 4151175 0.8552667 21.449213 25-29
CATCA 1687945 0.85267824 5.7897673 230-239
CCAAT 1681585 0.8494654 11.005657 240-247
CCAGC 2466565 0.8426713 5.6317677 80-89
CGGTC 3229370 0.8361962 5.5095925 130-139
AGCGT 2584070 0.83364844 6.4670067 160-169
GTATG 2410435 0.8232223 5.3123 220-229
GGGCC 3717440 0.81107575 16.321833 15-19
GACCG 2651245 0.807952 17.630936 45-49
CTTCC 2344325 0.8076274 5.5348196 230-239
CGTCC 2762980 0.8020437 9.498732 70-79
TAACC 1583020 0.7996746 6.1140814 80-89
AACCG 1817865 0.7737766 5.7618575 190-199
TGGGC 3294005 0.76082486 17.725094 15-19
GGTCC 2930180 0.75872546 12.501831 50-59
TGAGA 1884500 0.757466 5.897853 240-247
AAAGG 1592810 0.7534879 8.704873 10-14
AAAGA 1122340 0.7415748 6.055095 8
AGCCA 1740115 0.7406821 5.7817984 80-89
GTCGA 2241205 0.7230365 11.748262 70-79
AATCC 1430380 0.7225673 9.843387 240-247
CATAC 1425645 0.7201754 9.252634 70-79
ATGAT 1500140 0.7156022 7.2163277 240-247
CTAAT 1334575 0.7136952 9.809764 240-247
GTAGC 2207450 0.7121468 5.5222845 80-89
AAGGT 1757140 0.7062742 9.759063 10-14
AAAGC 1291715 0.68502903 9.8180485 70-79
GGAAG 2013535 0.68195117 6.2609777 30-34
CATTG 1763235 0.6750902 5.409124 50-59
TGTTA 1664395 0.6746072 5.0040817 230-239
AGACC 1555835 0.6622431 5.144474 240-247
AGGAT 1613100 0.648378 5.8548207 10-14
ATCCC 1584180 0.6423076 5.121081 240-247
TAGGT 1875005 0.6403599 5.57939 10-14
CCCAT 1569435 0.63632923 5.1265316 240-247
CAATC 1233930 0.6233291 9.412671 240-247
GAGGT 2160560 0.6217489 6.771861 10-14
ATAGG 1545660 0.6212708 5.2806787 10-14
TATGA 1299270 0.6197825 5.76184 240-247
CCGTA 1709395 0.61823136 5.2176914 80-89
CGATG 1895985 0.61166483 13.477626 15-19
AGAAG 1292220 0.61129206 5.658088 9
AATGG 1454690 0.5847058 6.6731224 10-14
ACGAT 1282885 0.5780756 6.073155 10-14
GGGTC 2443770 0.5644439 5.6233554 70-79
CATCC 1388795 0.56308854 7.6641116 240-247
AAATG 989530 0.5555385 5.4138465 9
TAAAA 703915 0.5519805 6.4052167 8
GAAAA 834070 0.55110335 5.7879353 8
GAAGG 1576905 0.5340718 5.4666786 10-14
AAGCA 985800 0.5227946 6.6219945 70-79
AAGAA 784070 0.51806635 5.277865 9
AAGGG 1501345 0.50848085 6.264661 10-14
TGATC 1288490 0.4933245 5.7949514 240-247
GGTCG 2100405 0.48513597 5.705896 70-79
GATCC 1312620 0.47473103 5.8617063 240-247
ATAAG 826575 0.4640529 5.4818654 9
TAAGG 1098580 0.4415691 5.9248285 10-14
CGGTT 1607320 0.44059107 7.9715705 10-14
GAAGC 1090860 0.41418374 5.053275 30-34
AAGCT 898330 0.40479282 8.311514 30-34
TAAAG 711810 0.39962193 5.640799 9