FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_1_SL248338.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_1_SL248338.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6426955
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7433125 9.122534 62.943783 110-119
ATACA 6904455 7.9736705 38.506958 110-119
CACAG 10042270 7.7856903 43.11901 110-119
TCCCT 11598855 7.20023 107.23615 30-34
CAGTA 8317665 6.8530164 40.056335 110-119
AGTAA 6506405 6.728099 49.126053 110-119
GGAGA 10372135 6.4473042 97.19286 35-39
GTAAT 7038255 6.1625514 40.646214 110-119
GGTGA 11419065 6.0101414 102.998436 45-49
CCCTG 11290840 5.90561 70.15644 30-34
AATAC 4962865 5.731408 40.81257 110-119
GTAGT 9005500 5.6254177 33.268433 80-89
AGACG 8005640 5.5575533 104.07795 40-44
ACAGT 6640700 5.4713464 36.417423 110-119
GTGAC 8839145 5.1956763 86.17576 45-49
TAGTA 5780590 5.0613647 43.384216 80-89
GAGAC 7059080 4.900447 144.28485 40-44
CCCCA 7082240 4.885877 102.55951 60-69
CTCAG 7345585 4.8220954 35.52784 140-149
ACTCC 6448875 4.72793 120.24642 30-34
AGTAG 6145490 4.5337677 34.84348 80-89
ACCAG 5804540 4.5002127 92.07957 45-49
CGTGT 9033385 4.4960027 23.453304 120-129
GCACA 5795845 4.493471 34.937366 110-119
GGCCC 10093600 4.4482775 58.342007 60-69
GTGTC 8905015 4.4321117 23.57684 120-129
TGACC 6711850 4.406073 147.66734 45-49
CACTC 5971795 4.3781633 99.37831 25-29
CTCCC 7267880 4.245451 68.91657 30-34
ATGCA 5010140 4.1279097 97.28384 25-29
CCCAG 6608125 4.0819955 88.15851 60-69
GACCA 5133670 3.9800923 105.67412 45-49
TGAGG 7472870 3.9331603 70.45364 35-39
TACAC 4262205 3.9218621 28.294048 110-119
AGGAG 6169030 3.8346596 97.57434 35-39
CCCTT 6148070 3.8165421 37.23096 20-24
AGATA 3682955 3.808445 20.17978 150-159
ATGGT 6095475 3.807628 26.578794 190-199
ACGGT 6431650 3.7805433 99.080444 40-44
GACGG 7598725 3.7633948 110.403435 40-44
CCACT 5082900 3.7264786 29.759443 240-247
CTGAG 6322010 3.7160964 106.29274 35-39
CAGGG 7422080 3.6759086 34.523205 50-59
GAGGA 5847790 3.6349778 127.75394 35-39
TGTCC 6529625 3.6294591 20.500633 120-129
TGCAC 5418555 3.557074 116.335976 25-29
TGGTG 7857440 3.5017045 18.330004 190-199
GCCCC 7093865 3.491454 73.12496 60-69
TCTTG 5903055 3.486949 18.924166 170-179
CTTGG 6984150 3.4760785 21.341076 20-24
CCGTG 7409455 3.4701414 23.482471 120-129
CCTGA 5264800 3.4561398 85.95128 30-34
GGGTT 7707030 3.4346738 40.86346 50-59
CTCTC 5479775 3.401684 13.946419 130-139
GGATG 6429700 3.3841136 71.00129 20-24
AGGCT 5749550 3.3796027 26.507385 30-34
CAGAT 4070505 3.3537343 18.430418 150-159
TCAGG 5662275 3.3283021 21.176445 140-149
GCACT 5057480 3.320042 90.261986 25-29
CTTCA 4255650 3.3156455 17.325542 200-209
TCGCA 5018775 3.2946339 24.482023 100-109
CCAGG 5848175 3.2347226 39.029007 50-59
TGTCT 5470575 3.2314823 20.634995 180-189
CGCAC 5165980 3.191149 24.867449 100-109
TCTCA 4083715 3.181688 22.136477 130-139
ACAGA 3220135 3.1333594 15.142549 140-149
CGGAT 5267880 3.0964756 80.51165 20-24
GGTTG 6912035 3.0803802 73.43306 15-19
CCTGG 6543960 3.064796 28.423157 50-59
GATGC 5059195 2.9738102 96.22565 25-29
GACTC 4497110 2.9521804 20.30086 190-199
ACCAC 3395130 2.9396746 20.565945 230-239
GGTTC 5898790 2.9358847 46.054626 50-59
TTCCC 4720165 2.9301405 47.95162 50-59
GCCCT 5533470 2.8942504 19.793386 20-24
TTCTT 4119325 2.8879364 21.845802 170-179
TGGCC 6158390 2.884218 42.853294 60-69
TCTCT 4342120 2.864496 15.149259 180-189
AGGGT 5438810 2.8625832 49.027336 50-59
GTTGG 6389695 2.8475971 52.99375 15-19
TCAAA 2433230 2.8100371 57.41023 70-79
GCCGT 5972210 2.7970228 22.186535 120-129
TCACA 3030530 2.788538 12.358589 140-149
CGGTG 6643445 2.785971 69.4567 40-44
CGGCC 6305310 2.7787673 12.944587 120-129
GATGG 5276995 2.7774158 20.600584 15-19
TCCAT 3440995 2.6809347 21.254436 70-79
AGATG 3575930 2.638103 17.360018 180-189
AGGGA 4237780 2.634198 9.3770075 150-159
AGTCA 3183895 2.6232464 30.393524 70-79
CCAGT 3992985 2.621242 34.95943 60-69
GTTCC 4706305 2.6159759 52.02614 50-59
TGTTC 4332030 2.5589406 13.181794 140-149
TGGAG 4858005 2.5568907 18.089373 25-29
ACAGC 3289065 2.5499852 11.480824 120-129
GCGGA 5132550 2.5419805 87.72572 20-24
TCCTC 4056070 2.5178897 16.899574 130-139
GGCTG 5967395 2.5024655 13.694077 30-34
ACTCT 3195340 2.4895413 14.29745 200-209
GTTCT 4193280 2.476981 18.320528 160-169
GGCGG 6903155 2.4391482 41.91186 20-24
CAGCT 3702970 2.4308581 12.772039 150-159
GAGAT 3293045 2.4294076 16.9017 180-189
TACCA 2601475 2.3937438 17.221413 230-239
TTGGG 5355440 2.3866763 79.940056 15-19
TGTAG 3814130 2.3825524 8.537262 210-219
CACGG 4290590 2.3731966 9.981825 120-129
CTACT 3013355 2.3477535 8.645798 230-239
CATTT 2827405 2.3410213 18.204885 150-159
CTGGA 3973645 2.3357203 14.856487 180-189
AATAA 1608980 2.3321178 9.736363 110-119
ACTAC 2525210 2.3235688 14.221305 230-239
TCATT 2794845 2.3140624 17.024954 150-159
TTCAT 2784505 2.3055012 16.392101 140-149
GATAC 2760725 2.274592 15.214698 150-159
CAGAG 3272390 2.2717087 9.787094 140-149
CTGGC 4809310 2.2523904 33.294617 60-69
GTGAA 3040365 2.242996 18.347328 190-199
TCAGC 3411140 2.2392828 14.6875515 140-149
CACTA 2408190 2.2158928 16.693491 240-247
TTGTC 3710880 2.1920257 13.35663 170-179
ATTTG 2952555 2.1889594 16.060038 150-159
TTCAC 2788340 2.1724405 18.857811 200-209
CTCGC 4133880 2.1622026 16.585852 100-109
ATGTA 2454225 2.1488686 9.533331 70-79
TACAG 2588455 2.1326568 14.497065 160-169
AGCTC 3246070 2.1309206 12.828619 140-149
TTGGA 3389045 2.1170168 16.350178 170-179
ACACA 1947695 2.11658 14.247904 110-119
GAACT 2550760 2.1015995 11.554581 160-169
TGGGG 5582640 2.0962605 52.63099 15-19
GATAT 2392200 2.0945609 8.989726 180-189
TCTCG 3748145 2.0833874 15.981068 100-109
CTCTG 3697170 2.0550532 14.702754 180-189
GTTCA 2942625 2.052861 15.049252 140-149
GCTGT 4115090 2.0481198 10.361984 140-149
TAGTC 2934190 2.046976 25.382051 70-79
ATATA 1665465 2.0439937 10.633562 110-119
GAATC 2479420 2.0428216 14.580464 190-199
TCTGC 3672925 2.0415766 12.270348 210-219
GTCTC 3665270 2.0373216 12.039908 180-189
GTCCT 3663650 2.0364213 14.360593 130-139
ACGGC 3674975 2.0326898 14.80734 120-129
ATATG 2315750 2.0276227 9.277763 180-189
CAGGC 3660290 2.0245671 15.32297 140-149
TTTGC 3417705 2.0188465 12.388784 150-159
AACCA 1852715 2.0133643 15.296216 70-79
TACTA 2057825 2.0122483 11.559133 230-239
TGAAT 2290755 2.005738 14.119995 190-199
AATCG 2432195 2.0039124 13.529272 190-199
TTGCA 2870715 2.0026941 14.573589 150-159
GACGT 3405655 2.0018542 10.171606 170-179
ACACG 2581250 2.0012221 14.037196 120-129
ATCTC 2563880 1.9975601 7.8116994 100-109
AATAT 1621405 1.9899197 13.405299 110-119
CCAGA 2553130 1.979421 31.008858 60-69
GTCAA 2360170 1.9445702 38.721428 70-79
TGGAA 2632235 1.9419026 9.385996 170-179
CAGCC 3136825 1.9376916 10.373991 120-129
TCGGC 4085575 1.9134367 8.093833 130-139
CCTTC 3073110 1.907697 14.20165 200-209
AGAAC 1943940 1.8915553 12.282658 160-169
GAGTC 3203900 1.8832622 12.737322 210-219
CATAG 2285700 1.8832138 8.632066 210-219
GGGGC 5285680 1.8676325 47.835876 15-19
AGTAT 2126800 1.862182 12.993099 220-229
CTGTT 3097480 1.829689 12.967499 140-149
GTTGT 3440575 1.8197939 7.4304733 210-219
GGGCG 5149595 1.8195485 58.24146 20-24
CGTAG 3054095 1.7952062 9.9238 210-219
GCAGA 2575720 1.7880771 14.846432 150-159
CCTTG 3186430 1.7711611 24.24472 20-24
ATTGT 2384295 1.767664 8.485109 50-59
GGTCA 2973505 1.747835 7.91212 130-139
GAGAA 2002350 1.7446105 11.376116 160-169
AACTG 2111220 1.7394576 11.310203 160-169
TGACT 2476085 1.727389 12.233847 190-199
GTCAC 2610365 1.7136048 8.983512 140-149
TGCAG 2908515 1.7096338 13.350504 150-159
AGTCT 2450265 1.709376 13.5824175 210-219
GTCCC 3242570 1.696008 23.128136 50-59
TGGTT 3195155 1.6899861 18.346512 10-14
ATAGT 1919070 1.680298 7.6576076 220-229
ACTGA 2038750 1.6797489 9.442774 180-189
CAAAA 1231140 1.6791604 22.574017 70-79
TCCCA 2262850 1.6589864 11.582086 240-247
CTCCA 2241005 1.642971 7.473704 150-159
CACCA 1884805 1.6319592 13.277813 230-239
GAACC 2103875 1.6311172 12.601794 70-79
TTGGT 3080905 1.6295565 25.52365 25-29
GCTCA 2472255 1.6229409 11.431987 140-149
TTGTA 2186050 1.6206893 9.606893 210-219
AATAG 1565895 1.61925 10.5586815 240-247
TTCAG 2309750 1.6113486 9.771977 150-159
GGACG 3234165 1.601774 7.497418 170-179
CTGGT 3218150 1.6017041 7.224888 160-169
TAGTT 2155115 1.597755 10.475195 220-229
GCTCC 3046905 1.5936664 6.097907 150-159
TCAGA 1932060 1.5918455 9.996719 130-139
GTGCT 3190630 1.5880072 8.7632065 220-229
CAGAC 2045225 1.5856464 19.176596 60-69
GGCCG 3986915 1.5732768 13.018998 120-129
TGTGC 3160645 1.5730835 8.406766 220-229
TCTGG 3155705 1.5706247 11.944485 180-189
GTCTG 3151965 1.5687633 9.303396 210-219
ACCAT 1696395 1.5609356 12.991276 45-49
TGCTC 2808185 1.5609154 5.122027 150-159
CTGTG 3112945 1.5493426 5.354773 210-219
ATAGA 1496910 1.5479145 11.021927 240-247
GCTCT 2734565 1.5199941 10.301067 130-139
CAAAG 1552720 1.5108776 27.833742 70-79
CCTCG 2879600 1.5061586 9.689487 130-139
TGATA 1717820 1.5040876 8.443706 180-189
CCCAC 2172640 1.4988552 10.977184 240-247
CATGT 2124685 1.4822421 9.4694805 70-79
ACCGT 2249390 1.4766386 22.78206 45-49
CCACC 2138820 1.4755237 9.764478 230-239
CGACT 2240215 1.4706154 8.976748 200-209
GCTGA 2464005 1.4483495 22.595987 30-34
TCGAC 2198545 1.4432607 9.635786 190-199
AATTG 1629195 1.4264894 9.544918 170-179
CTCTT 2153930 1.4209474 8.772501 200-209
TATAC 1453020 1.4208385 6.987207 110-119
TGGAC 2411640 1.4175693 10.504032 170-179
TATGG 2240790 1.3997422 6.718676 190-199
AAATA 964010 1.3972733 5.3030357 9
GGGAG 3142660 1.3936664 6.7265596 150-159
CACTG 2105170 1.3819636 7.70515 230-239
CTCGA 2091280 1.3728454 8.72689 190-199
GAGGG 3087850 1.36936 8.494116 200-209
CTGAT 1959575 1.3670565 7.8878756 180-189
GTCCA 2078580 1.3645083 19.66786 70-79
CCATG 2052115 1.3471351 9.53526 70-79
GCGTA 2283980 1.3425303 10.41612 210-219
GTGGA 2541540 1.3376765 22.859648 25-29
ACGGA 1920940 1.3335258 9.498497 200-209
GAATT 1521755 1.3324171 10.59904 170-179
ACGTG 2266490 1.3322495 9.742501 170-179
CCTCA 1816305 1.3316065 7.092317 130-139
GTAGG 2515855 1.3241581 5.0038166 160-169
CTTGA 1894455 1.3216268 8.941628 200-209
AGCCG 2370585 1.3112098 9.174714 120-129
AGATC 1578495 1.3005395 6.985447 130-139
CCATC 1768495 1.2965549 11.181567 240-247
TTGGC 2592835 1.2904788 9.23282 60-69
ACTTC 1655100 1.2895151 6.7902465 220-229
ACTAT 1315340 1.2862079 6.958199 230-239
ACTCG 1959115 1.286084 9.004373 190-199
GTGTT 2424650 1.2824495 7.5885816 160-169
CTGCT 2299790 1.2783266 5.7301826 90-99
TGGTC 2566725 1.2774838 14.554175 50-59
GGCTC 2722825 1.2752069 8.595285 130-139
GTGGT 2850565 1.2703674 12.884515 50-59
GGAGG 2844800 1.2615752 17.085323 30-34
GATCT 1805975 1.2599006 5.6027503 130-139
ACTGG 2142735 1.2595061 7.4944487 160-169
TCCAC 1714860 1.2572328 10.117069 240-247
AGCTT 1801320 1.2566532 6.8891244 150-159
AAAAA 729560 1.2488681 8.508308 8
ATAAC 1078065 1.2450129 7.3927803 80-89
ATCGG 2115260 1.2433561 8.068507 190-199
AAGTA 1199830 1.240712 15.770579 70-79
TTGAG 1980015 1.2368453 8.655567 200-209
GGAGT 2317950 1.2199956 7.1789465 210-219
GGAAT 1652560 1.2191579 8.760396 170-179
CCATA 1321600 1.2160685 12.328629 70-79
ATACC 1312280 1.2074927 9.028609 70-79
TGCCC 2305910 1.2060932 8.104721 60-69
TCTAC 1546470 1.2048796 8.101165 180-189
AGAGC 1728905 1.200214 7.5547156 140-149
GTCTA 1695005 1.1824847 8.110259 180-189
AAAGT 1135230 1.1739109 20.365578 70-79
ATAAA 802970 1.1638558 5.6823063 7
TCACG 1771655 1.1630237 9.534775 200-209
GCATA 1392685 1.1474485 7.919903 210-219
GCTTC 2050370 1.1396878 6.048127 150-159
TACTG 1633005 1.1392317 7.095175 180-189
GAGCT 1935380 1.1376221 6.7815533 140-149
TATCA 1154465 1.1288959 7.474557 70-79
AGTTC 1617160 1.1281778 5.616109 160-169
AAAAG 921660 1.1255835 15.55226 70-79
ACTGT 1610090 1.1232457 5.8461413 230-239
AGGTT 1790605 1.1185277 23.694334 10-14
TTCCA 1434465 1.1176147 7.780708 230-239
CTCGG 2349335 1.1002866 8.3957815 130-139
CGGCT 2343505 1.0975562 8.001701 130-139
ATGGG 2057835 1.0830905 32.573498 15-19
CAATA 936275 1.0812654 6.523772 100-109
ATATC 1094755 1.0705084 7.932934 70-79
ATCCA 1163015 1.0701467 16.272663 240-247
TCTGT 1808875 1.068507 6.392423 210-219
AGAGA 1225815 1.0680301 14.271591 35-39
CTACC 1447490 1.0612133 9.076958 230-239
GAAGA 1213410 1.0572218 18.909239 35-39
TATGT 1425930 1.0571532 8.067043 220-229
CTGCG 2249290 1.0534316 7.156381 210-219
GTCCG 2220880 1.0401261 5.216209 130-139
GCGGT 2448180 1.02666 5.9144464 130-139
AACTT 1049660 1.0264121 6.050598 200-209
ATACG 1238625 1.0205169 5.4423165 120-129
CTGAA 1237830 1.0198618 15.473397 35-39
CGAAC 1307725 1.0138686 13.224164 70-79
TAGAT 1143500 1.0012249 8.394361 240-247
TATAA 813025 0.9978102 6.9953184 240-247
CATGG 1695795 0.99679345 5.3951917 190-199
CTGCC 1903300 0.99551034 5.6204586 200-209
TCCCC 1689825 0.98709244 5.382456 80-89
CGTGG 2351165 0.985976 8.820279 50-59
TGCGT 1980265 0.9855969 8.413992 210-219
ACGTC 1500250 0.9848568 13.508173 70-79
AAAAT 676770 0.98093647 5.353857 8
GGGGT 2607120 0.978964 7.537293 10-14
CAGCG 1738915 0.96182275 6.4661393 160-169
AGCTG 1631140 0.95878893 5.1936073 90-99
CCCAA 1087155 0.94131356 10.658512 240-247
CTTCT 1425760 0.9405737 5.4814863 210-219
ACTAA 814160 0.94024 13.484266 240-247
ACAAT 812470 0.93828815 7.595317 100-109
GAGTT 1499550 0.9367159 5.418676 160-169
CCATT 1200920 0.93565625 6.352225 50-59
CGGAG 1885265 0.9337088 6.7278004 210-219
ACCCA 1065140 0.9222519 13.367626 240-247
AAGAG 1057220 0.9211362 14.239272 35-39
TGTAA 1051145 0.9203609 7.1564116 230-239
TAGGC 1553575 0.9131961 5.601396 160-169
TCGAA 1105520 0.91085017 17.105814 70-79
TGAAG 1234230 0.9105397 10.237405 35-39
CGGTC 1891955 0.88607746 5.7389755 130-139
ATCAA 762090 0.88010633 14.156834 70-79
TGCAT 1252380 0.8736967 6.121345 210-219
CATCA 949280 0.8734788 5.497002 230-239
CGTCC 1667795 0.8723308 10.963574 70-79
TACAA 750700 0.8669525 5.015942 100-109
GGTGG 2300185 0.86371094 22.49792 25-29
GACCG 1548730 0.85662824 20.608027 45-49
GAGGC 1727385 0.8555161 14.510133 30-34
CCAAT 923035 0.8493295 11.232444 240-247
AGCGT 1427350 0.8390006 6.6244516 160-169
TGAAC 1013930 0.83538824 6.666365 190-199
GGTCC 1760280 0.8244089 14.480228 50-59
GGGCC 2077635 0.81985563 17.006807 15-19
CCAGC 1322480 0.81692743 5.6021647 80-89
TTCTG 1376630 0.8131787 5.3033404 210-219
AACCG 1040890 0.80699354 5.8634377 190-199
GTATG 1289340 0.80540514 5.595285 220-229
TAACC 858430 0.7898832 5.8983207 80-89
CTTCC 1264335 0.7848622 5.5677814 230-239
TGGGC 1854595 0.77773625 18.53785 15-19
CATAC 840955 0.77380365 10.824157 70-79
GGTGC 1823675 0.7647698 5.2206793 220-229
CTAAT 761775 0.74490315 10.220246 240-247
AAAGG 846490 0.73753107 9.007517 10-14
ATGAT 832340 0.7287797 7.116458 240-247
AATCC 791360 0.72816885 10.064439 240-247
AGCCA 932990 0.7233395 5.7788744 80-89
GTAGC 1229350 0.72261566 5.673578 80-89
CCTTT 1087480 0.7174105 5.629566 50-59
AAAGA 586035 0.7156994 5.8479905 8
AAAGC 732660 0.71291643 10.544475 70-79
GTCGA 1210205 0.7113621 11.7469425 70-79
TGAGA 957625 0.70647734 6.04375 240-247
TGTTA 931250 0.6904083 5.2319894 230-239
AAGGT 935175 0.68991506 10.142517 10-14
CATTG 972615 0.67852455 5.8154135 50-59
TAGGT 1085180 0.6778736 5.504635 10-14
TATGA 759725 0.66519946 6.294781 240-247
CCGTA 1007495 0.66138196 5.6611295 80-89
TCCAG 997070 0.6545383 5.077455 240-247
CAATC 697250 0.64157367 9.8474865 240-247
CGATG 1061315 0.62384415 14.278512 15-19
GAGGT 1182760 0.62251645 6.8812714 10-14
AGGAT 842675 0.6216742 6.2725496 10-14
GGAAG 971500 0.6038829 6.218574 30-34
ATAGG 808050 0.59612995 5.3620524 10-14
AGAAG 677375 0.5901844 5.404893 9
AGACC 757910 0.5876015 5.210525 240-247
CATCC 772675 0.56647915 7.621394 240-247
AATGG 764390 0.5639203 6.732951 10-14
AAGCA 560140 0.5450455 7.1176972 70-79
TAAAA 373040 0.5406986 6.190016 8
GGGTC 1277420 0.5356943 5.5805044 70-79
ACGAT 632545 0.52116084 6.5815325 10-14
AAATG 503820 0.52098674 5.538031 9
AAGAA 420675 0.5137523 5.4565477 9
AAGGG 806445 0.50128496 6.256004 10-14
GAAGG 799140 0.49674428 5.5634046 10-14
TTGCC 886985 0.4930261 5.7687793 60-69
GAAAA 394095 0.4812913 6.2039294 8
GGTCG 1134805 0.4758877 5.672832 70-79
TGATC 681285 0.47528425 5.2620616 240-247
TACCG 703650 0.4619193 5.727287 70-79
ATAAG 441720 0.4567708 5.7833633 9
GATCC 682500 0.44803515 5.2502394 240-247
TAAGG 580840 0.4285083 5.976336 10-14
CGGTT 852740 0.4244169 8.141093 10-14
GAAGC 598150 0.4152386 5.052157 30-34
AAGCT 494540 0.407457 8.555893 30-34
TAAAG 372490 0.38518193 5.615242 9