FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_12_SL248341.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_12_SL248341.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9179605
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10374620 8.846859 62.613956 110-119
ATACA 9462610 7.6326456 38.41428 110-119
CACAG 13883935 7.584515 42.513382 110-119
TCCCT 16429020 7.2984858 108.00816 30-34
CAGTA 11847790 6.8423505 39.77574 110-119
AGTAA 9054160 6.498523 48.242283 110-119
GGAGA 14433320 6.242899 92.81744 35-39
GTAAT 10109110 6.1721087 40.30857 110-119
CCCTG 16427305 6.142386 70.247475 30-34
GGTGA 16026990 5.896933 101.2834 45-49
AATAC 6888115 5.55603 40.45143 110-119
AGACG 10915265 5.3058124 100.47817 40-44
ACAGT 9130005 5.2727714 36.21286 110-119
GTAGT 11987860 5.2404213 26.688599 80-89
GTGAC 12314175 5.0918674 83.7965 45-49
ACTCC 9361690 4.8890133 123.24242 30-34
GAGAC 10027875 4.8744607 140.2842 40-44
CCCCA 9792650 4.8374276 101.55308 60-69
ACCAG 8727130 4.767456 94.77748 45-49
CTCAG 10139975 4.7120104 35.65185 140-149
GCACA 8260215 4.512389 34.807487 110-119
CACTC 8566740 4.4738617 99.72922 25-29
TAGTA 7290200 4.4510255 34.10727 80-89
GGCCC 14056600 4.4238534 59.158684 60-69
GTGTC 12472010 4.386947 24.069921 120-129
TGACC 9433680 4.3837976 143.59824 45-49
CGTGT 12407475 4.364247 23.496712 120-129
CTCCC 10174095 4.2752757 70.1079 30-34
AGTAG 8224890 4.2266903 27.882982 70-79
ATGCA 7225705 4.172998 96.80589 25-29
GACCA 7599480 4.151443 107.049324 45-49
CCCAG 9220775 4.053069 86.49127 60-69
TGAGG 10478040 3.8552654 68.26511 35-39
TACAC 5830505 3.7841797 28.109499 110-119
AGGAG 8738625 3.7797508 93.278786 35-39
CAGGG 10853985 3.7775543 36.03317 50-59
ACGGT 9035535 3.7361617 94.87848 40-44
GACGG 10651365 3.7070355 106.22153 40-44
CCACT 7086845 3.7010067 29.3904 240-247
CTGAG 8907355 3.6831596 104.77227 35-39
ATGGT 8424420 3.682685 26.403158 190-199
TGCAC 7880300 3.6619475 116.746895 25-29
TGTCC 9158925 3.6204956 20.943542 120-129
AGATA 5022750 3.6050234 19.62105 150-159
GAGGA 8228520 3.5591133 122.68332 35-39
GGGTT 11321350 3.5434523 42.823597 50-59
CCCTT 7925790 3.5209808 33.865562 20-24
CCTGA 7503865 3.4870195 85.045364 30-34
GCCCC 9823900 3.4745772 72.73768 60-69
CCAGG 8725395 3.4127474 41.036892 50-59
GCACT 7309460 3.39668 90.09055 25-29
GGATG 9182590 3.3786204 69.828514 20-24
TGGTG 10734325 3.35972 18.267906 190-199
TCTTG 8000645 3.3434997 18.49666 170-179
CTTCA 6037965 3.3335755 17.303211 200-209
CCGTG 9946980 3.3095157 23.77011 120-129
CTCTC 7436265 3.3035123 13.663327 130-139
AGGCT 7915150 3.2728865 23.463617 30-34
CTTGG 9294885 3.269415 18.843292 20-24
TCAGG 7884410 3.2601755 21.166168 140-149
CAGAT 5632400 3.2528305 17.995829 150-159
CCTGG 9753205 3.2450438 29.710163 50-59
TCGCA 6922550 3.2168846 24.452047 100-109
TGTCT 7550680 3.1554575 20.336529 180-189
CGGAT 7583450 3.1357293 79.69971 20-24
TTCCC 7057285 3.135153 50.9686 50-59
CGCAC 7114995 3.127456 25.033432 100-109
TCTCA 5652780 3.1209137 21.998514 130-139
GGTTG 9878810 3.0919535 74.22415 15-19
GGTTC 8634895 3.0372672 48.56781 50-59
ACAGA 4469255 3.034233 14.99628 140-149
GATGC 7310070 3.022688 97.33042 25-29
AGGGT 8115925 2.9861543 50.58483 50-59
TGGCC 8953435 2.9789476 43.63394 60-69
TCAAA 3600905 2.9045298 59.12155 70-79
AGTCA 4998010 2.8864567 32.609715 70-79
GTTGG 9192120 2.8770275 52.944393 15-19
GACTC 6147725 2.856826 20.47189 190-199
ACCAC 4651895 2.8558981 20.277843 230-239
CCAGT 6136055 2.851403 37.526165 60-69
TCTCT 6038925 2.836178 15.347866 180-189
GCCCT 7489000 2.8002355 18.276947 20-24
CGGTG 9454350 2.799028 66.60844 40-44
GCCGT 8388310 2.790922 22.45189 120-129
TTCTT 5587695 2.7743375 21.24632 170-179
GTTCC 6977760 2.7582877 55.180912 50-59
CGGCC 8587350 2.7025864 12.835836 120-129
GATGG 7335555 2.6990268 18.57324 15-19
TCACA 4118080 2.6727624 12.297457 140-149
AGATG 5080495 2.6108167 17.291883 180-189
GCGGA 7421315 2.5828686 87.2902 20-24
AGGGA 5910920 2.5566728 9.395095 150-159
TCCAT 4566180 2.5209992 16.62034 70-79
TCCTC 5631975 2.5019681 17.18451 130-139
TGTTC 5935960 2.4806597 13.171151 140-149
GGCTG 8375285 2.4795635 12.054931 30-34
GGCGG 9924470 2.4730487 41.942425 20-24
ACAGC 4476040 2.4451706 11.305327 120-129
TGGAG 6626315 2.4380708 15.855516 25-29
ACTCT 4399955 2.429226 14.342083 200-209
TTGGG 7745695 2.424313 79.71659 15-19
CTGGC 7254850 2.413802 35.402832 60-69
GAGAT 4645890 2.3874776 16.881538 180-189
CAGCT 5129960 2.3838742 12.688309 150-159
GTTCT 5691300 2.3784156 18.0452 160-169
TACCA 3622510 2.3511221 16.947035 230-239
AATAA 2335055 2.3407757 9.93169 110-119
CACGG 5926310 2.3179467 9.818788 120-129
CTGGA 5543190 2.2920895 14.693063 180-189
CATTT 3907975 2.2809944 17.966606 150-159
TCATT 3896350 2.2742093 16.800264 150-159
TCAGC 4887450 2.2711806 14.874577 140-149
TGTAG 5191930 2.269621 8.426039 210-219
TTCAT 3850045 2.2471821 16.529575 140-149
CTACT 4016180 2.2173429 7.8996377 230-239
CAGAG 4558250 2.2157245 9.862512 140-149
ACTAC 3372185 2.1886532 12.932886 230-239
TTCAC 3958870 2.1857018 19.017775 200-209
GTGAA 4217090 2.1671214 18.120188 190-199
GATAC 3751085 2.166331 14.789567 150-159
TTGTC 5130445 2.144032 13.481366 170-179
CACTA 3302725 2.1435716 16.015825 240-247
TGGGG 8102205 2.134427 52.175926 15-19
TAGTC 4343780 2.133978 26.90255 70-79
ATTTG 4099085 2.1289318 15.8083725 150-159
CTCGC 5689845 2.1275082 16.6574 100-109
GAACT 3680445 2.125535 11.52618 160-169
TCTGC 5350685 2.11511 12.448881 210-219
TCTCG 5349550 2.1146612 17.058067 100-109
ATCTC 3823080 2.1107318 9.02062 100-109
TTGGA 4767190 2.0839484 16.195261 170-179
GTCAA 3594405 2.0758452 41.40336 70-79
AGCTC 4446215 2.0661404 12.822932 140-149
GCTGT 5836125 2.0528183 10.231983 140-149
GTCCT 5172925 2.0448418 14.5406065 130-139
GATAT 3347200 2.0436301 8.540784 180-189
GTTCA 4158555 2.042982 15.342971 140-149
GTCTC 5151330 2.0363054 11.971373 180-189
ACACG 3724400 2.0345647 13.77682 120-129
GAATC 3515120 2.0300565 14.689566 190-199
ATATA 2380085 2.029595 10.467862 110-119
TACAG 3514290 2.029577 13.885202 160-169
ATGTA 3322915 2.028803 7.322215 160-169
CTCTG 5127445 2.0268633 14.695859 180-189
ACACA 2638135 2.012836 13.769628 110-119
CAGGC 5135140 2.0084977 15.4424925 140-149
AACCA 2609915 1.9913048 15.39063 70-79
AATAT 2326845 1.9841949 13.214788 110-119
AAAAA 1681205 1.9812042 10.983391 120-129
TGAAT 3244375 1.9808505 14.078408 190-199
TTGCA 4014085 1.972008 14.43789 150-159
ACGGC 5039755 1.9711899 14.6169195 120-129
AATCG 3395255 1.9608318 13.51209 190-199
CCTTC 4322295 1.9201514 14.256094 200-209
TGGAA 3732890 1.9182957 9.2913 170-179
TTTGC 4585925 1.9164754 12.320732 150-159
ATATG 3126775 1.9090501 8.828617 180-189
GGGGC 7651890 1.9067512 47.57418 15-19
TACTA 2777755 1.9059533 10.372005 230-239
CAGCC 4330960 1.9037099 10.308843 120-129
GAGTC 4555200 1.883559 12.527536 210-219
TCGGC 5606050 1.8652204 7.867408 130-139
GGGCG 7447755 1.8558835 59.09437 20-24
CATAG 3206935 1.852073 8.622866 210-219
GACGT 4333390 1.7918414 10.074425 170-179
AGAAC 2636315 1.789827 12.11336 160-169
GTCAC 3840010 1.7844391 8.96018 130-139
CCAGA 3265000 1.7836037 25.578161 60-69
CTGTT 4262880 1.7814736 12.930957 140-149
CTCCA 3401530 1.776402 8.013118 150-159
GTTGT 4759530 1.7698755 7.3667984 210-219
AGTAT 2898025 1.7693868 12.2443 220-229
CCTTG 4432385 1.7521083 21.644766 20-24
CGTAG 4196550 1.7352583 9.976066 210-219
GCAGA 3533940 1.7178165 14.569983 150-159
TTGTA 3302100 1.7150037 9.813688 210-219
TGCAG 4130870 1.7081001 13.691418 150-159
ATTGT 3284165 1.7056888 7.5404267 170-179
GGTCA 4124095 1.7052987 7.93724 130-139
TTCAG 3462995 1.701273 9.862046 150-159
CACCA 2739690 1.6819545 13.960642 230-239
AGTCT 3417440 1.6788929 13.517977 210-219
AACTG 2906495 1.6785625 11.3419 160-169
TGGTT 4511940 1.6778069 18.0222 10-14
TGACT 3398510 1.6695931 12.232647 190-199
GAGAA 2745915 1.6588355 10.959712 160-169
ATAGT 2707375 1.6529858 7.673766 220-229
TCAGA 2840445 1.6404173 10.171826 130-139
TCCCA 3136785 1.6381426 11.406846 240-247
GTCTG 4642815 1.6330798 9.265522 210-219
CAAAG 2388390 1.6215078 29.721676 70-79
CAAAA 1700420 1.6123755 21.119781 70-79
ACTGA 2774475 1.6023183 9.276748 180-189
GCTCC 4270580 1.5968261 6.4063396 150-159
TGCTC 4015260 1.5872203 5.289757 150-159
CTGGT 4500665 1.5830792 7.332847 160-169
AATAG 2203230 1.5813441 10.0830555 240-247
GAACC 2885320 1.5761923 12.687758 70-79
TCTGG 4465435 1.5706874 12.007101 180-189
CTGTG 4457270 1.5678154 5.400196 210-219
GCTCA 3372925 1.5673862 11.464292 140-149
ATAGA 2178085 1.5632967 10.819801 240-247
TAGTT 3009530 1.5630523 10.367371 220-229
GTGCT 4434480 1.5597991 8.484603 220-229
TGTGC 4433955 1.5596144 8.366173 220-229
TTGGT 4191185 1.5585312 23.221867 25-29
GTCCC 4161605 1.556079 19.291025 50-59
GGCCG 5534370 1.549855 13.144415 120-129
GGACG 4422405 1.5391467 7.4645715 170-179
ACCAT 2320445 1.5060412 11.31713 45-49
CCCAC 2992205 1.4781059 10.74552 240-247
CCACC 2989655 1.4768462 10.082589 230-239
CCTCG 3933765 1.4708867 9.69376 130-139
GCTCT 3714720 1.4684175 10.014589 130-139
CGACT 3115355 1.4476944 9.092462 190-199
CAGAC 2609655 1.4256021 14.762649 60-69
TCGAC 3053805 1.4190924 9.854881 190-199
TGATA 2321410 1.417335 8.030971 180-189
TATAC 2065390 1.4171647 6.6945105 110-119
CATGT 2880395 1.4150578 7.2964206 70-79
TGGAC 3388540 1.4011493 10.6839075 170-179
GCTGA 3381840 1.3983787 19.759697 30-34
AAATA 1394595 1.3980117 5.473388 9
CACTG 3006085 1.3969172 7.5953712 230-239
CTCTT 2973815 1.3966506 8.6726885 200-209
AATTG 2273860 1.3883035 9.346291 170-179
CTGCT 3492675 1.380644 5.824483 90-99
AGATC 2385455 1.3776509 7.1188087 130-139
GGGAG 4407850 1.3650568 6.7209973 150-159
GAGGG 4381315 1.3568392 8.471483 200-209
CCTCA 2589065 1.3521034 7.4214296 130-139
CTTGA 2734020 1.3431479 8.868974 200-209
GCGTA 3244215 1.3414713 10.469427 210-219
CTCGA 2859760 1.3289204 8.81854 190-199
CTGAT 2699510 1.326194 7.735013 180-189
GTAGG 3569275 1.3132706 5.1767282 160-169
CCATC 2501035 1.3061309 11.204788 240-247
ACGGA 2683355 1.3043549 9.460605 200-209
ACTTC 2357360 1.3015043 6.8925138 220-229
TATGG 2967575 1.2972575 6.2916055 180-189
GAATT 2118295 1.2933233 10.339298 170-179
ATCGG 3119395 1.2898587 7.9673767 190-199
CCATG 2763290 1.2840911 7.3926663 70-79
GTGGA 3487525 1.2831917 20.05722 25-29
ACGTG 3092225 1.278624 9.591374 170-179
AGCCG 3263195 1.2763273 9.123481 120-129
TCCAC 2443295 1.275977 10.040291 240-247
GGAAT 2477530 1.2731786 8.640492 170-179
AAAGT 1759540 1.2628906 21.647312 70-79
ACTAT 1833655 1.2581601 6.5949993 230-239
ACCGT 2696210 1.2529193 17.315752 45-49
GTGTT 3360850 1.2497634 7.653166 160-169
AAGTA 1730955 1.2423738 15.957257 70-79
ACTCG 2668620 1.2400984 9.0236 190-199
GGCTC 3697305 1.2301512 8.313411 130-139
GATCT 2502345 1.2293322 5.626226 130-139
GGAGT 3328485 1.2246749 7.114252 210-219
ATAAC 1512800 1.2202412 7.6994686 80-89
ACTGG 2931010 1.2119622 7.5180364 160-169
GGAGG 3907880 1.2102221 15.379908 30-34
AGAGC 2487890 1.2093412 7.4498663 140-149
GTCCA 2602100 1.2091867 15.510231 70-79
TTGAG 2747155 1.2009023 8.5553465 200-209
AGCTT 2438080 1.1977607 6.60003 150-159
ATAAA 1187055 1.1899632 5.138783 7
TTGGC 3351890 1.1790051 7.8049726 60-69
GTGGT 3729560 1.1673094 9.947476 50-59
GCATA 2010930 1.1613548 8.294163 210-219
TGGTC 3301185 1.16117 11.370355 50-59
TATCA 1675225 1.1494535 6.8460875 70-79
ATATC 1670865 1.146462 7.380851 70-79
TTCCA 2075065 1.1456485 7.787669 230-239
GAAGA 1881565 1.1366727 18.199158 35-39
TGCCC 3037630 1.1358099 6.3129983 60-69
ATACC 1740545 1.129668 7.0185347 70-79
TCTAC 2037325 1.1248121 7.820337 180-189
ACTGT 2288805 1.1244261 5.8840246 230-239
GTCTA 2277965 1.1191008 7.776787 180-189
AGGTT 2558390 1.1183848 22.931648 10-14
TCACG 2401780 1.1160988 9.706407 200-209
AGTTC 2263600 1.1120435 5.4172583 160-169
CAATA 1376535 1.1103283 6.626197 100-109
CCATA 1687635 1.0953276 9.786825 70-79
CTGAA 1896430 1.0952287 14.236382 35-39
TCTGT 2620300 1.0950333 6.621647 210-219
GCTTC 2767145 1.0938442 5.859722 150-159
TACTG 2203845 1.0826876 6.8727465 180-189
GAGCT 2616415 1.0818783 6.6313047 140-149
TGTAA 1769340 1.0802691 7.2812133 230-239
AAAAG 1279995 1.0799923 13.943086 70-79
CGGCT 3233700 1.0759025 7.82367 130-139
ATCCA 1653585 1.0732282 16.285635 240-247
CTCGG 3220140 1.0713909 8.407181 130-139
GTCCG 3148865 1.0476766 5.188488 130-139
TATGT 1998620 1.0380185 7.7145042 220-229
GCGGT 3488435 1.0327761 5.9167466 130-139
AACTT 1504485 1.0323006 6.2104745 200-209
AGAGA 1704895 1.0299447 12.66665 35-39
CTGCG 3092760 1.0290096 6.991035 210-219
CTTCT 2185335 1.0263414 5.654916 210-219
TAGAT 1674290 1.0222363 8.44609 240-247
CTGCC 2731040 1.0211719 5.95417 200-209
GGGGT 3865650 1.0183582 7.7266283 10-14
CTACC 1932890 1.0094252 8.880796 230-239
CATGG 2439695 1.0088052 5.414842 190-199
ATGGG 2741740 1.0087893 29.054329 15-19
ATACG 1740150 1.0049735 5.162573 120-129
CGAAC 1803250 0.9850792 13.39726 70-79
AGAAA 1164400 0.98245937 5.1584997 6
AAGAG 1626025 0.9822983 13.560765 35-39
TATAA 1149165 0.97993946 6.7155986 240-247
CAGCG 2490270 0.9740146 6.371156 160-169
AAAAT 963615 0.9659758 5.4913025 8
TGCGT 2740630 0.9639985 8.340569 210-219
ACAAT 1176975 0.949361 7.7612453 100-109
TGCTA 1930500 0.9484009 5.0106635 220-229
AGCTG 2293380 0.9483045 5.121357 90-99
TCCCC 2253175 0.94681096 5.105053 80-89
ACCCA 1531205 0.94003963 13.377201 240-247
TAGGC 2251995 0.9311919 5.8445616 160-169
CGGAG 2650745 0.9225489 6.6559267 210-219
TCGAA 1592010 0.91941965 17.04748 70-79
CCATT 1660130 0.9165619 5.7793665 50-59
GAGTT 2083200 0.9106584 5.1101604 160-169
AGTTT 1747170 0.90742344 5.0947976 220-229
CCCAA 1473345 0.90451807 10.345844 240-247
TTCTG 2160230 0.90276825 5.5570836 210-219
TGAAC 1556020 0.89863455 6.644541 190-199
CGTGG 3032080 0.89766896 6.902762 50-59
ACGTC 1923035 0.8936276 10.642148 70-79
ACTAA 1097750 0.8854573 12.777862 240-247
TGCAT 1795440 0.8820496 6.361142 210-219
CTGTA 1789400 0.87908244 5.001365 230-239
TGAAG 1709800 0.8786495 9.280106 35-39
CCAGC 1964255 0.8634048 5.839414 80-89
CGGTC 2574230 0.85648644 5.767952 130-139
GTATG 1950180 0.85250944 5.3707724 220-229
AGCGT 2056615 0.850403 6.5966477 160-169
CCAAT 1309015 0.8495915 11.265049 240-247
CATCA 1302385 0.8452885 5.842838 230-239
ATCAA 1043975 0.8420817 12.873516 70-79
GAGGC 2380445 0.8284754 12.974826 30-34
GGTGG 3139465 0.8270537 20.106218 25-29
CTTCC 1808120 0.8032456 5.5032187 230-239
TAACC 1221910 0.79305774 6.2193503 80-89
GGGCC 2786190 0.7802497 15.113253 15-19
GACCG 1991760 0.7790333 15.962106 45-49
AACCG 1413170 0.77198637 5.830126 190-199
TCCAG 1652510 0.76791555 5.050875 240-247
AAAGG 1260260 0.761336 8.675277 10-14
CGTCC 2024945 0.7571536 8.7099695 70-79
AGCCA 1385985 0.7571357 6.046696 80-89
AAAGA 896920 0.7567738 5.892024 8
TGAGA 1469510 0.7551669 5.746402 240-247
GGTGC 2530680 0.74922603 5.091628 220-229
GTCGA 1778555 0.7354262 12.166396 70-79
ATGAT 1194545 0.72932845 7.351853 240-247
TGGGC 2458120 0.7277441 16.463793 15-19
AATCC 1113475 0.72268003 10.087523 240-247
GGTCC 2164945 0.72031105 11.550966 50-59
CTAAT 1044305 0.7165486 9.856777 240-247
AAGGT 1380495 0.70942295 9.817482 10-14
GTAGC 1698400 0.70228237 5.525378 80-89
GGAAG 1597635 0.69103116 5.9531865 30-34
AAAGC 994515 0.6751887 9.567444 70-79
TGTTA 1296740 0.67348474 5.0381227 230-239
CATAC 1036680 0.6728377 8.383228 70-79
CATTG 1335780 0.65623146 5.2225366 50-59
TAGGT 1484380 0.6488878 5.331438 10-14
TATGA 1054365 0.64374167 6.041443 240-247
AGGAT 1244715 0.6396469 5.430003 10-14
ATCCC 1215975 0.63502616 5.058763 240-247
CAATC 975380 0.63305205 9.776498 240-247
GAGGT 1706345 0.62782854 6.727127 10-14
CCCAT 1194175 0.6236414 5.056915 240-247
AGAAG 1012745 0.6118096 5.169545 9
ATAGG 1187460 0.6102241 5.1583986 10-14
CGATG 1433820 0.5928795 13.0046015 15-19
GGGTC 1996225 0.5909968 5.8349485 70-79
CCGTA 1271480 0.59085226 5.011537 80-89
AATGG 1122815 0.57700366 6.5075693 10-14
GAAAA 679870 0.5736385 6.1454334 8
CATCC 1052530 0.54966927 7.6104 240-247
TAAAA 538760 0.54007995 6.1846123 8
GAAGG 1245440 0.5386949 5.445036 10-14
AAGAA 627355 0.52932906 5.2286553 9
AAGGG 1196015 0.51731694 6.2172656 10-14
AAGCA 750460 0.5094967 6.290761 70-79
GGTCG 1681340 0.4977728 5.9038 70-79
TGATC 986050 0.48441884 5.636392 240-247
GATCC 1005955 0.46746373 5.7581606 240-247
ATAAG 646100 0.4637312 5.338206 9
ACGAT 779510 0.45018357 5.805827 10-14
CGGTT 1267835 0.44595253 8.0174265 10-14
TAAGG 866565 0.4453193 5.7478385 10-14
TAAAG 559270 0.4014099 5.46872 9
AAGCT 675510 0.39012134 7.5169225 30-34