FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_11_SL248340.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_11_SL248340.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11989916
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13553110 8.885319 62.662464 110-119
ATACA 12366240 7.655103 38.833405 110-119
CACAG 18056680 7.54677 42.28596 110-119
TCCCT 21457115 7.2894263 107.88574 30-34
CAGTA 15424945 6.8275914 39.67959 110-119
AGTAA 11802620 6.5041866 48.324017 110-119
GGAGA 18860275 6.24703 94.47279 35-39
CCCTG 21430985 6.119905 70.51368 30-34
GTAAT 12874240 6.035124 40.20063 110-119
GGTGA 20955105 5.9042587 100.57175 45-49
AATAC 9015955 5.5811687 40.937588 110-119
AGACG 14347760 5.338368 101.1354 40-44
GTAGT 15845835 5.311414 27.858421 80-89
ACAGT 11911510 5.272428 35.828762 110-119
GTGAC 16211030 5.1307983 83.97385 45-49
GAGAC 13147385 4.891744 140.62093 40-44
ACTCC 12140165 4.8483796 121.90003 30-34
CCCCA 12854175 4.8472514 101.63802 60-69
CTCAG 13334385 4.740741 35.622547 140-149
ACCAG 11288400 4.7179747 95.25695 45-49
TAGTA 9658765 4.527788 35.78991 80-89
GCACA 10736845 4.487453 34.274143 110-119
CACTC 11127185 4.4438286 100.10687 25-29
GGCCC 18389125 4.4141192 58.84838 60-69
GTGTC 16362250 4.405217 24.105616 120-129
TGACC 12363335 4.395506 144.40768 45-49
CGTGT 16215840 4.3657994 23.65331 120-129
AGTAG 10853295 4.276679 28.671387 80-89
CTCCC 13290060 4.263127 69.88117 30-34
ATGCA 9377635 4.1508512 96.80364 25-29
GACCA 9850825 4.1171417 107.63655 45-49
CCCAG 12094110 4.0600038 86.61919 60-69
TGAGG 13687220 3.856477 68.57434 35-39
TACAC 7645375 3.8013818 28.297857 110-119
AGGAG 11419220 3.7823524 94.838776 35-39
CAGGG 14070740 3.743448 35.43979 50-59
ACGGT 11807380 3.7370412 96.75699 40-44
CCACT 9294060 3.7117395 28.84638 240-247
CTGAG 11696325 3.7018926 104.65532 35-39
GACGG 13885310 3.6941152 107.326744 40-44
ATGGT 11013300 3.6915817 26.199533 190-199
TGCAC 10202580 3.6272979 116.35264 25-29
TGTCC 11947790 3.6133556 20.766775 120-129
AGATA 6548215 3.6085896 19.581924 150-159
CCCTT 10515680 3.5723944 35.151005 20-24
GAGGA 10762040 3.5646768 123.52562 35-39
GGGTT 14680950 3.5186791 42.336464 50-59
GCCCC 12905890 3.4799247 72.65946 60-69
CCTGA 9785585 3.4790447 85.592224 30-34
TGGTG 14158865 3.3935473 18.122524 190-199
GGATG 12035945 3.3912182 70.2486 20-24
CCAGG 11314620 3.3813772 40.43089 50-59
GCACT 9459640 3.3631625 90.40744 25-29
TCTTG 10403460 3.3321278 18.34375 170-179
CCGTG 13092395 3.3283024 23.640171 120-129
AGGCT 10478630 3.3164911 24.606998 30-34
CTCTC 9710760 3.2989461 13.836796 100-109
CTTGG 12247095 3.297292 19.978777 20-24
CTTCA 7792730 3.2959628 17.06649 200-209
CAGAT 7422010 3.2852275 18.182251 150-159
TCAGG 10287990 3.2561538 21.102581 140-149
CCTGG 12687735 3.2254312 29.795107 50-59
TCGCA 9033875 3.211791 24.495602 100-109
TGTCT 9856140 3.1568263 20.050137 180-189
TCTCA 7387505 3.1245716 22.184763 130-139
CGCAC 9281675 3.115867 24.953922 100-109
CGGAT 9829950 3.111184 80.14957 20-24
TTCCC 9139985 3.105042 50.614174 50-59
GGTTG 12822365 3.07322 73.50097 15-19
ACAGA 5819845 3.0283446 14.904012 140-149
GGTTC 11166215 3.0062861 48.00385 50-59
GATGC 9490940 3.0038872 96.37231 25-29
AGGGT 10527050 2.9660757 50.1421 50-59
TGGCC 11660640 2.9643266 43.346973 60-69
TCAAA 4660205 2.8848178 58.56842 70-79
GACTC 8070995 2.8694608 20.599152 190-199
GTTGG 11923250 2.8577232 52.814384 15-19
AGTCA 6442925 2.851852 32.107254 70-79
ACCAC 6069615 2.849595 20.251762 230-239
TCTCT 7869415 2.8312948 15.068488 180-189
GCCCT 9906820 2.8290253 18.68099 20-24
CCAGT 7927645 2.818496 37.39419 60-69
CGGTG 12368765 2.7991827 67.98788 40-44
GCCGT 10954990 2.784939 22.258112 120-129
TTCTT 7254160 2.7640789 20.848928 170-179
GTTCC 9057285 2.739184 54.581512 50-59
GATGG 9661325 2.722151 19.051157 15-19
CGGCC 11258620 2.7025154 12.807721 120-129
TCACA 5340785 2.6555095 12.076747 140-149
AGATG 6593620 2.5981784 17.39627 180-189
TCCAT 6141745 2.597673 17.41997 70-79
GCGGA 9623070 2.5601685 87.043365 20-24
AGGGA 7714320 2.55519 9.424831 150-159
TCCTC 7362770 2.5012853 16.949402 130-139
GGCTG 11039580 2.4983737 12.7173195 30-34
TGTTC 7677760 2.4591124 13.113935 140-149
GGCGG 12880155 2.4502277 41.824936 20-24
TGGAG 8692100 2.4490645 16.759388 25-29
ACTCT 5784030 2.4463763 14.248673 200-209
CACGG 8098165 2.4201388 9.837131 120-129
ACAGC 5774215 2.413327 11.057668 120-129
TTGGG 10038990 2.4061103 79.483444 15-19
CTGGC 9412875 2.3929079 34.921688 60-69
CAGCT 6720035 2.3891575 12.631415 150-159
GAGAT 6023515 2.3735318 16.903233 180-189
GTTCT 7403170 2.3711636 18.172028 160-169
TACCA 4748065 2.3608012 17.049177 230-239
AATAA 3031420 2.3363085 9.917523 110-119
TGTAG 6925110 2.3212488 8.536565 210-219
CTGGA 7226580 2.287216 14.512851 180-189
TCAGC 6404600 2.2770114 15.140241 140-149
CATTT 5045835 2.2601974 17.601494 150-159
TCATT 5033795 2.2548044 16.397686 150-159
TTCAT 4946415 2.215664 16.327154 140-149
CAGAG 5929445 2.2061675 9.690868 140-149
CTACT 5209070 2.2031949 7.8139377 180-189
ACTAC 4378125 2.1768618 12.753654 230-239
TTCAC 5111375 2.1618743 18.80263 200-209
GTGAA 5463055 2.1526854 17.823437 190-199
TTGTC 6706340 2.147976 13.47555 170-179
GATAC 4841095 2.142829 14.774445 150-159
CACTA 4297995 2.13702 15.4652 240-247
TAGTC 5666780 2.1336844 26.665863 70-79
GAACT 4798135 2.1238134 11.437511 160-169
CTCGC 7433835 2.122831 16.776794 100-109
ATTTG 5314415 2.1191902 15.444407 150-159
TGGGG 10502280 2.115873 52.30379 15-19
ATCTC 4989310 2.1102464 8.868969 100-109
TCTGC 6969055 2.107643 12.300678 210-219
TCTCG 6968780 2.10756 16.950344 100-109
TTGGA 6232115 2.0889616 16.236132 170-179
ATGTA 4448265 2.0852358 7.8689227 70-79
AGCTC 5848565 2.0793257 13.0231085 140-149
ACGGC 6955740 2.078725 14.505301 120-129
GTCAA 4661570 2.0633652 41.060024 70-79
ATATA 3129450 2.0516443 10.484766 110-119
ACACG 4900615 2.0482068 13.646738 120-129
GCTGT 7594375 2.0446377 9.954742 140-149
GTCCT 6732400 2.0360718 14.259316 130-139
GATAT 4337335 2.0332348 8.597133 180-189
CTCTG 6712395 2.0300217 14.564449 180-189
TACAG 4585165 2.0295455 13.641821 160-169
GTCTC 6708735 2.028915 11.830952 180-189
GAATC 4551210 2.0145164 14.482229 190-199
GTTCA 5334635 2.0086234 15.226094 140-149
ACACA 3435880 2.0083098 14.013207 110-119
CAGGC 6703600 2.003373 15.259352 140-149
AACCA 3412710 1.9947668 15.458668 70-79
AATAT 3004345 1.9696264 13.087062 110-119
AATCG 4431910 1.9617099 13.676383 190-199
TGAAT 4183100 1.9609333 13.908405 190-199
TTGCA 5185070 1.9523084 14.196089 150-159
TGGAA 4918290 1.9380239 9.282055 170-179
TTTGC 6013585 1.9260932 12.05499 150-159
CCTTC 5593705 1.9002973 14.045316 200-209
ATATG 4037750 1.8927969 8.871484 180-189
TACTA 3580755 1.8855498 10.181276 230-239
CAGCC 5615440 1.8851085 10.090706 120-129
GGGGC 9904930 1.8842424 47.599205 15-19
AAAAA 2072100 1.8773482 9.968016 120-129
GAGTC 5926515 1.8757448 12.4036 210-219
CATAG 4198445 1.8583705 8.716929 210-219
TCGGC 7240980 1.8407763 7.7624044 130-139
GGGCG 9660765 1.8377942 58.352566 20-24
GACGT 5757370 1.8222102 10.176616 170-179
CCAGA 4339665 1.8137581 26.402393 60-69
AGAAC 3462740 1.8018298 11.885265 160-169
CTCCA 4476705 1.7878476 8.247985 150-159
GTCAC 4988950 1.7737089 9.083075 130-139
CTGTT 5514785 1.7663324 12.724708 140-149
GTTGT 6190265 1.7650387 7.41004 210-219
AGTAT 3764005 1.764472 12.071752 220-229
CGTAG 5481525 1.7349054 9.915832 210-219
ATTGT 4326805 1.7253683 7.6509824 50-59
GGTCA 5434080 1.7198889 7.937807 130-139
GCAGA 4582495 1.7050078 14.410753 150-159
TGACT 4516025 1.7003964 12.362422 190-199
CCTTG 5598510 1.6931508 22.776989 20-24
TGCAG 5347350 1.6924388 13.436712 150-159
TTCAG 4472395 1.6839687 9.660668 150-159
AACTG 3799405 1.6817423 11.232111 160-169
TCAGA 3799060 1.6815898 10.648877 130-139
AGTCT 4453115 1.6767093 13.277165 210-219
CACCA 3562215 1.6724076 13.86581 230-239
TCCCA 4173100 1.6665978 11.581646 240-247
TGGTT 5839720 1.6650872 17.945538 10-14
ATAGT 3550570 1.6644189 7.671318 220-229
GAGAA 3590215 1.663089 10.831943 160-169
GTCTG 6025915 1.6223606 9.096811 210-219
GCTCC 5672035 1.6197256 6.5804095 150-159
TGCTC 5337815 1.6143091 5.53675 150-159
CAAAA 2215590 1.6123259 21.253347 70-79
CTGTG 5981285 1.6103448 5.6150217 210-219
CAAAG 3083230 1.6043526 29.284157 70-79
AATAG 2907225 1.6021134 9.939386 240-247
ACTGA 3618580 1.6017034 9.284457 180-189
TAGTT 4008180 1.5983125 10.545011 220-229
TGTGC 5934185 1.5976639 8.547296 220-229
CTGGT 5906500 1.5902102 7.284764 160-169
TTGTA 3985990 1.5894641 9.659953 210-219
GTCCC 5557995 1.5871599 19.864174 50-59
TTGGT 5557120 1.5845093 23.813183 25-29
GCTCA 4442705 1.5795039 11.678756 140-149
ATAGA 2856600 1.5742148 10.826712 240-247
GTGCT 5823600 1.567891 8.568418 220-229
GAACC 3748975 1.5668801 12.747787 70-79
GGCCG 7305290 1.5610673 13.149494 120-129
TCTGG 5784215 1.5572873 11.837868 180-189
GGACG 5756300 1.5314339 7.488438 170-179
ACCAT 3070880 1.5268823 11.592063 45-49
CCCAC 3931305 1.4824774 10.622475 240-247
CATGT 3893975 1.466179 7.7231245 70-79
CCACC 3877340 1.4621274 9.948164 230-239
GCTCT 4824555 1.4590845 10.130706 130-139
CCTCG 5088945 1.4532164 9.408911 130-139
CAGAC 3459720 1.4459864 15.4402895 60-69
GCTGA 4560030 1.4432516 20.968365 30-34
CGACT 4039430 1.4361286 9.1482725 190-199
TATAC 2708235 1.4260991 6.6903486 110-119
TGGAC 4461000 1.4119085 10.773927 170-179
TGATA 3004745 1.4085495 8.11242 180-189
CACTG 3959395 1.4076738 7.7741165 230-239
AAATA 1820380 1.4029627 5.1466436 9
TCGAC 3945275 1.4026538 9.759023 190-199
AGATC 3163770 1.4003894 7.3632483 130-139
CTCTT 3879085 1.3956352 8.591091 200-209
AATTG 2963535 1.3892316 9.488196 170-179
GGGAG 5818270 1.3780023 6.763667 150-159
CTGCT 4554650 1.3774575 5.740248 90-99
CCTCA 3441985 1.3746147 7.523883 130-139
GAGGG 5718915 1.354471 8.575921 200-209
GCGTA 4225725 1.3374441 10.478326 210-219
CTGAT 3546075 1.335186 7.681315 180-189
CTTGA 3531795 1.3298091 8.745354 200-209
CCATG 3739980 1.3296658 7.7961683 70-79
GTGGA 4705905 1.3259242 21.171083 25-29
CCATC 3317910 1.3250632 11.686959 240-247
GTAGG 4698450 1.3238236 5.296729 160-169
ACTTC 3126890 1.3225292 6.962874 220-229
CTCGA 3704520 1.3170588 8.783591 190-199
GAATT 2762170 1.2948366 10.47606 170-179
ACGGA 3479310 1.294546 9.489429 200-209
TATGG 3860150 1.2938955 6.3881726 180-189
TCCAC 3215310 1.2840881 10.179758 240-247
ACGTG 4055780 1.2836564 9.840311 170-179
ACCGT 3608170 1.2828037 18.383615 45-49
GGAAT 3208240 1.2641886 8.628172 170-179
ATCGG 3986300 1.2616659 7.9990344 190-199
AGCCG 4208955 1.2578475 8.954092 120-129
AAGTA 2279640 1.2562637 16.125265 70-79
AAAGT 2275970 1.2542415 21.572388 70-79
ACTAT 2370815 1.248421 6.508695 230-239
GATCT 3315260 1.2482784 5.840588 130-139
GTGTT 4368595 1.2456234 7.5528936 160-169
GTCCA 3482980 1.2382952 16.167253 70-79
ACTCG 3476240 1.235899 9.026203 190-199
GGCTC 4844265 1.2314919 8.347205 130-139
GGAGG 5181870 1.2272769 15.852063 30-34
ATAAC 1981030 1.2263218 7.688431 80-89
ACTGG 3858440 1.2211981 7.4719 160-169
GGAGT 4323155 1.2180815 7.016258 210-219
TCACG 3414120 1.2138135 9.774812 200-209
AGCTT 3208245 1.2079846 6.459318 150-159
TGGTC 4447050 1.1972816 11.825106 50-59
GTGGT 4993085 1.1967252 10.394876 50-59
TTGAG 3566775 1.1955582 8.437636 200-209
AGAGC 3210470 1.1945188 7.3094563 140-149
TTGGC 4393860 1.1829613 8.021578 60-69
TGCCC 4107085 1.1728331 6.628867 60-69
GCATA 2643010 1.1698837 8.437113 210-219
ATAAA 1515205 1.167765 5.373939 7
ATACC 2315745 1.1514192 7.3385563 70-79
ACTGT 3053125 1.149578 5.9205523 230-239
TATCA 2178000 1.1468886 6.896658 70-79
TTCCA 2707520 1.1451552 7.773783 230-239
CGGCT 4491410 1.1417904 7.697108 130-139
TCTGT 3511960 1.1248468 6.8251934 210-219
TCTAC 2656615 1.1236248 7.59991 180-189
CCATA 2259115 1.1232619 10.22534 70-79
GAAGA 2410950 1.1168202 18.153578 35-39
GTCTA 2963320 1.1157641 7.6807017 180-189
ATATC 2116560 1.1145357 7.40709 70-79
AGGTT 3317740 1.1120836 23.160423 10-14
AGTTC 2940075 1.1070118 5.409242 160-169
CAATA 1781385 1.102735 6.6915593 100-109
ATCCA 2213810 1.1007358 16.830425 240-247
GCTTC 3613130 1.0927147 5.8194375 150-159
CTGAA 2461920 1.0897273 14.333951 35-39
GAGCT 3436935 1.0877914 6.599662 140-149
TACTG 2864880 1.078699 6.760695 180-189
AAAAG 1659825 1.0752932 14.068926 70-79
CTCGG 4182680 1.0633062 8.166092 130-139
AACTT 1997470 1.0518254 6.3554816 200-209
GTCCG 4101700 1.0427197 5.117117 130-139
CTTCT 2896785 1.0422188 5.763847 210-219
ATGGG 3697520 1.041804 30.179182 15-19
CTGCC 3631865 1.0371277 6.0256705 200-209
CATGG 3266330 1.033795 5.442585 190-199
GCGGT 4564445 1.0329823 5.9899735 130-139
TATGT 2589675 1.0326657 7.5473638 220-229
AGAGA 2227630 1.0319011 12.850038 35-39
TAGAT 2180835 1.0223212 8.306375 240-247
CTGCG 4011200 1.0197132 6.873794 210-219
CTACC 2541665 1.0150568 8.769583 230-239
GGGGT 5012935 1.0099459 7.772221 10-14
ATACG 2239085 0.99109304 5.097001 120-129
CGAAC 2345520 0.98030764 13.271055 70-79
AAAAT 1266055 0.97574574 5.37271 8
AAGAG 2101420 0.97343713 13.606594 35-39
TATAA 1477275 0.9684905 6.7505727 240-247
TGCGT 3586845 0.9656882 8.385197 210-219
CAGCG 3225450 0.9639268 6.2617517 160-169
TAGGC 3022175 0.9565198 5.987253 160-169
AGCTG 3013510 0.9537774 5.122869 90-99
ACAAT 1527940 0.9458444 7.7584596 100-109
TCCCC 2945310 0.94478357 5.013749 80-89
ACCCA 2001470 0.9396608 13.521221 240-247
TGTAA 1984995 0.9305162 7.3720555 230-239
CCATT 2194510 0.9281758 5.8668823 50-59
AGTTT 2325280 0.9272349 5.1642046 220-229
CGTGG 4095800 0.92692286 7.1619053 50-59
CCCAA 1948865 0.91496354 10.520666 240-247
TTCTG 2854105 0.91414225 5.685154 210-219
TCGAA 2063080 0.91318756 16.9588 70-79
CGGAG 3431370 0.9128984 6.5322084 210-219
ACGTC 2560705 0.91040105 11.069003 70-79
GAGTT 2699105 0.9047214 5.1376114 160-169
TGCAT 2378240 0.89546686 6.4888325 210-219
TGAAG 2257010 0.8893619 9.328271 35-39
ACTAA 1434345 0.887906 12.574053 240-247
CTGTA 2357890 0.8878046 5.0829577 230-239
TGAAC 1986150 0.8791358 6.3675847 190-199
CGGTC 3381985 0.8597563 5.755374 130-139
CCAAT 1728245 0.85930634 11.213477 240-247
GAGGC 3191180 0.84899706 13.525673 30-34
CCAGC 2526940 0.84829605 5.666037 80-89
GGTGG 4205470 0.84726757 20.845905 25-29
CATCA 1698195 0.8443652 5.8399796 230-239
ATCAA 1352780 0.8374146 12.789357 70-79
GTATG 2493165 0.83569163 5.295281 220-229
AGCGT 2626055 0.8311476 6.45968 160-169
CTTCC 2351075 0.7987088 5.4557676 230-239
TAACC 1603040 0.79705274 6.192847 80-89
GGGCC 3725800 0.7961661 15.952535 15-19
GACCG 2645125 0.79049647 16.896338 45-49
CGTCC 2728345 0.77911544 9.069946 70-79
AACCG 1835920 0.7673208 5.6778073 190-199
TGAGA 1921230 0.7570497 5.991294 240-247
AAAGG 1634290 0.75704944 8.6368885 10-14
AAAGA 1162295 0.75297576 5.282731 8
TGGGC 3308975 0.748856 17.346294 15-19
TCCAG 2098575 0.74610114 5.105672 240-247
AGCCA 1781035 0.7443817 5.9815273 80-89
GGTGC 3284960 0.74342126 5.030922 220-229
GGTCC 2913525 0.7406661 11.984588 50-59
GTCGA 2318325 0.73375094 12.025237 70-79
AATCC 1464175 0.72800726 10.125465 240-247
ATGAT 1543570 0.7235873 7.312778 240-247
CTAAT 1352430 0.712161 9.739843 240-247
GTAGC 2242525 0.7097602 5.5129848 80-89
AAGGT 1781355 0.7019328 9.996536 10-14
CATAC 1390950 0.69159883 8.7357 70-79
GGAAG 2059055 0.68201435 5.868862 30-34
AGACC 1627550 0.6802328 5.180937 240-247
TGTTA 1698435 0.6772725 5.04654 230-239
AAAGC 1290590 0.671556 9.667628 70-79
CATTG 1779395 0.6699867 5.3293123 50-59
CAATC 1297215 0.64499253 9.87093 240-247
TAGGT 1916185 0.6422919 5.39939 10-14
ATCCC 1607680 0.64205414 5.1653876 240-247
AGGAT 1625390 0.64047563 5.824312 10-14
CCCAT 1603415 0.6403508 5.264922 240-247
TATGA 1333175 0.62495923 5.8748035 240-247
GAGGT 2203325 0.6208034 6.7097964 10-14
ATAGG 1559060 0.6143387 5.1452994 10-14
CGATG 1912570 0.60532933 13.183115 15-19
AGAAG 1304370 0.60422105 5.4153767 9
CCGTA 1699235 0.6041248 5.1204495 80-89
AATGG 1483575 0.58459425 6.712535 10-14
GGGTC 2550655 0.57724017 5.6717215 70-79
GAAAA 890715 0.57703656 6.1066074 8
CATCC 1422145 0.5679576 7.8735924 240-247
AAATG 1012475 0.55795467 5.669741 9
TAAAA 697845 0.53782755 5.686203 8
GAAGG 1622045 0.5372649 5.5237236 10-14
AAGAA 802050 0.5195963 5.3701363 9
AAGGG 1566460 0.51885366 6.363389 10-14
AAGCA 976150 0.5079377 6.3689404 70-79
TGATC 1334380 0.50242746 5.864194 240-247
GGTCG 2180135 0.49338767 5.78391 70-79
GATCC 1361215 0.48394936 5.938927 240-247
ATAAG 840775 0.46333423 5.5253606 9
ACGAT 1025860 0.45407963 6.1626086 10-14
TAAGG 1126540 0.44390666 5.969128 10-14
CGGTT 1632945 0.4396387 8.121313 10-14
TAAAG 732690 0.40377077 5.722483 9
AAGCT 888915 0.39346322 7.7677317 30-34