FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_13_SL248342.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_13_SL248342.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10675014
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12061735 8.882446 61.71345 110-119
ATACA 11088760 7.688627 37.4152 110-119
CACAG 16376350 7.639002 41.831203 110-119
TCCCT 18794355 7.0789957 102.377846 30-34
CAGTA 13445745 6.661337 39.13564 110-119
AGTAA 10562820 6.5435877 47.704525 110-119
GGAGA 16838410 6.269908 94.70401 35-39
GTAAT 11426365 6.012315 39.50611 110-119
GGTGA 18727610 5.9229765 101.6377 45-49
CCCTG 18372330 5.821323 66.644646 30-34
AATAC 8056215 5.5859475 39.441696 110-119
AGACG 13335145 5.5576034 102.97156 40-44
ACAGT 10894090 5.3971877 35.67642 110-119
GTAGT 14311435 5.380561 32.311344 80-89
GTGAC 14528945 5.1430583 85.2192 45-49
CCCCA 11742780 4.9029846 102.45685 60-69
GAGAC 11745075 4.89492 143.18561 40-44
TAGTA 9267190 4.876202 42.119064 80-89
CTCAG 11989490 4.7502637 34.773163 140-149
ACTCC 10381455 4.603678 115.603485 30-34
GGCCC 16941830 4.5157623 58.17867 60-69
ACCAG 9516110 4.4389367 89.52266 45-49
GCACA 9484675 4.4242735 34.242775 110-119
TGACC 11141300 4.4142094 146.45782 45-49
GTGTC 14642020 4.402363 23.58938 120-129
AGTAG 9915025 4.388748 33.7422 80-89
CGTGT 14423360 4.3366194 23.010494 120-129
CACTC 9534585 4.228132 94.48266 25-29
CTCCC 11600355 4.113945 66.18138 30-34
CCCAG 10946570 4.083551 87.43078 60-69
ATGCA 7987430 3.95716 92.679504 25-29
GACCA 8474755 3.9531806 103.542336 45-49
CCCTT 10376960 3.908538 42.363148 20-24
TGAGG 12090140 3.8237457 69.05395 35-39
TACAC 6823355 3.7835913 27.041206 110-119
AGGAG 10160490 3.7833345 94.88836 35-39
ACGGT 10600525 3.752448 97.24297 40-44
GACGG 12588265 3.7485774 108.91929 40-44
ATGGT 9843195 3.700671 25.90186 190-199
TGTCC 10960000 3.6882904 20.418 120-129
AGATA 5952675 3.6876376 19.479624 150-159
CTGAG 10377165 3.6733816 104.50922 35-39
CAGGG 12038440 3.5848484 33.54582 50-59
GAGGA 9605015 3.5764992 124.88286 35-39
CCACT 8056810 3.5728092 27.88929 240-247
GCCCC 11799865 3.5202823 73.15266 60-69
CTTGG 11672220 3.509444 24.181013 20-24
TGGTG 13030070 3.500273 19.13933 25-29
TGCAC 8767125 3.4735553 110.84943 25-29
AGGCT 9809940 3.4725912 29.887442 30-34
CCGTG 12164170 3.4435809 23.083826 120-129
GGATG 10525500 3.3288972 67.060234 20-24
CCTGA 8394810 3.3260434 81.225586 30-34
CAGAT 6706175 3.322396 17.880602 150-159
TCTTG 9277600 3.3159444 18.030365 170-179
CTCTC 8780895 3.3073716 13.638993 100-109
GGGTT 12309280 3.306647 39.558186 50-59
TCAGG 9241780 3.27147 20.823702 140-149
CCAGG 9635125 3.2113497 38.1703 50-59
GCACT 8058550 3.1928167 85.67778 25-29
CTTCA 6768960 3.1880543 16.662815 200-209
TCGCA 8036560 3.1841042 23.649128 100-109
TCTCA 6691350 3.1515014 21.679556 130-139
TGTCT 8747575 3.1265063 19.708712 180-189
CCTGG 11000010 3.1140165 27.916948 50-59
ACAGA 5337390 3.1132069 14.411978 140-149
CGCAC 8285995 3.0910401 24.129387 100-109
GCCCT 9441350 2.9915175 22.607538 20-24
CGGAT 8434520 2.9857104 76.24463 20-24
GGTTG 10982595 2.950259 70.35151 15-19
TGGCC 10274895 2.9087424 42.51457 60-69
TTCCC 7685920 2.8949435 47.077515 50-59
GATGG 9139815 2.8906472 23.215775 15-19
TCAAA 4143020 2.8726501 56.86641 70-79
GACTC 7229510 2.8643486 19.459478 190-199
GATGC 8090320 2.8638685 92.13344 25-29
TCTCT 7131370 2.8528192 15.06172 180-189
GGTTC 9453070 2.8422203 44.763783 50-59
CGGTG 11121940 2.813058 68.19731 40-44
AGGGT 8876950 2.8075109 47.311646 50-59
TCACA 4993515 2.768934 11.762975 140-149
ACCAC 5302190 2.7682447 18.775284 230-239
GCCGT 9740970 2.757592 21.783733 120-129
TTCTT 6459345 2.7443962 20.650974 170-179
TCCAT 5821535 2.7418346 21.062185 70-79
GTTGG 10132310 2.7218468 50.618553 15-19
CGGCC 10165935 2.7096803 12.434435 120-129
AGTCA 5403735 2.677137 29.637299 70-79
AGATG 5971985 2.6434162 17.177101 180-189
GGCTG 10352860 2.6185353 15.450164 30-34
TGGAG 8275740 2.6173663 20.410553 25-29
GTTCC 7724275 2.5993948 50.809467 50-59
CCAGT 6528700 2.5866861 33.807594 60-69
AGGGA 6808565 2.5352201 9.028122 150-159
TCCTC 6721165 2.5315633 16.86945 130-139
TGTTC 6928090 2.4761968 12.808192 140-149
ACTCT 5211820 2.4546702 13.967253 200-209
GCGGA 8208570 2.4443765 83.262024 20-24
GAGAT 5479420 2.425389 16.704226 180-189
ACAGC 5185825 2.4190083 11.16951 120-129
AATAA 2788315 2.4175036 10.69586 110-119
GGCGG 11135015 2.3692024 39.97354 20-24
TGTAG 6266610 2.3560095 8.034308 210-219
GTTCT 6588100 2.3546796 17.261402 160-169
CTGGA 6598470 2.3357728 14.51323 180-189
CAGCT 5857710 2.3208387 12.125594 150-159
CACGG 6924170 2.3077989 9.640202 120-129
TTGGG 8537875 2.2935328 76.10348 15-19
CATTT 4571075 2.286542 17.712967 150-159
TCATT 4536125 2.2690592 16.497192 150-159
TACCA 4085240 2.26529 15.959494 230-239
CAGAG 5419760 2.2587588 9.23721 140-149
CTGGC 7965650 2.2550132 32.812386 60-69
TTCAT 4503485 2.252732 15.891632 140-149
GTGAA 4957245 2.1942556 17.883934 190-199
TTGTC 6139215 2.1942418 13.159531 170-179
TCAGC 5497405 2.1780846 14.0708065 140-149
GATAC 4381435 2.1706655 14.477748 150-159
ATGTA 4105000 2.1599653 9.422825 70-79
TTCAC 4557220 2.146366 18.433783 200-209
ATTTG 4795290 2.1431177 15.581829 150-159
CTACT 4539200 2.1378787 7.7196684 230-239
CTCGC 6695055 2.1213465 16.203373 100-109
ACTAC 3822950 2.1198487 12.52965 230-239
GAATC 4231355 2.0963123 14.799109 190-199
GCTGT 6879570 2.0684555 9.952909 140-149
CTCTG 6143985 2.0675912 14.431255 180-189
GAACT 4168950 2.0653954 10.794548 160-169
GTCCT 6120795 2.0597875 14.318364 130-139
TCTCG 6118585 2.0590436 15.803275 100-109
ATCTC 4368005 2.057249 8.045079 100-109
CACTA 3695395 2.0491188 14.869016 240-247
TGAAT 3893160 2.0484996 14.283143 190-199
TACAG 4133405 2.0477855 13.516356 160-169
CCAGA 4385315 2.0455978 31.623432 60-69
TCTGC 6075950 2.0446959 12.019444 210-219
TTGGA 5427065 2.0403721 15.31718 170-179
AGCTC 5137795 2.0356064 12.091277 140-149
ACACA 3114605 2.033348 13.715283 110-119
GTCTC 6039040 2.032275 11.751103 180-189
CAGGC 6068215 2.0225124 15.225296 140-149
GTTCA 4801495 2.0204613 14.820954 140-149
TGGGG 8913010 2.0141547 49.83989 15-19
AATCG 4062935 2.0128732 13.5825615 190-199
TTTGC 5598150 2.0008576 11.892516 150-159
TAGTC 4739905 1.9945445 24.144688 70-79
ACGGC 5953495 1.9842768 14.312851 120-129
TTGCA 4714620 1.9839045 14.062869 150-159
GATAT 3769190 1.983269 8.140069 180-189
ATATA 2689470 1.980567 10.420196 110-119
GTCAA 3985245 1.9743837 37.865 70-79
TGGAA 4449080 1.9693235 8.765277 170-179
ACACG 4206535 1.9622035 13.582375 120-129
AATAT 2654855 1.9550759 13.096543 110-119
AACCA 2974585 1.9419367 14.592014 70-79
CCTTG 5766315 1.9404967 27.529043 20-24
GACGT 5468040 1.9356151 10.01598 60-69
CAGCC 5111590 1.9068478 10.133588 120-129
ATATG 3623125 1.9064128 8.375213 180-189
CCTTC 5018375 1.8901981 13.665219 200-209
GAGTC 5336900 1.8891934 12.231558 210-219
TCGGC 6627490 1.8761905 7.513184 130-139
CATAG 3784295 1.8748283 8.527983 210-219
TACTA 3167150 1.8652279 10.434473 240-247
CTGTT 5038325 1.8007683 12.46115 140-149
GGGGC 8441690 1.7961425 45.28492 15-19
GTTGT 5598900 1.7879059 7.002062 210-219
AGAAC 3058225 1.7838094 11.459678 160-169
AGTAT 3354905 1.7652808 11.928692 220-229
GTCCC 5557125 1.7607905 23.650604 50-59
GGGCG 8228235 1.7507255 55.732464 20-24
CGTAG 4930870 1.7454638 9.63042 210-219
GCAGA 4159475 1.7335179 14.214538 150-159
ATTGT 3862970 1.726444 8.665854 50-59
GGTCA 4873750 1.7252443 7.5434194 130-139
CTCCA 3870415 1.7163435 7.906058 150-159
TGCAG 4832200 1.710536 13.186843 150-159
TTGGT 5338840 1.7048606 29.048521 25-29
TCAGA 3437325 1.7029312 10.504369 130-139
GTCAC 4297225 1.7025706 8.566897 140-149
CAAAA 2073680 1.692817 22.805977 70-79
TGACT 4015690 1.689796 11.811391 190-199
ATAGT 3187830 1.6773696 7.5896254 220-229
CAGAC 3569145 1.6648828 19.274113 60-69
AGTCT 3949895 1.6621094 12.91305 210-219
TCCCA 3732430 1.6551538 11.527732 240-247
AACTG 3333105 1.6512983 10.629479 160-169
GAGAA 3165110 1.649446 10.645857 160-169
CACCA 3143685 1.6413009 13.175323 230-239
TGGTT 5107275 1.6309143 17.43323 10-14
CTGTG 5419615 1.629496 5.5478535 210-219
AATAG 2616965 1.6211903 9.568443 240-247
TTCAG 3826515 1.6101913 9.233728 150-159
GCTCC 5010340 1.58754 6.321413 150-159
ACCAT 2857395 1.5844426 13.263968 45-49
ATAGA 2556310 1.5836151 10.469272 240-247
TGCTC 4702345 1.5824465 5.470762 150-159
GCTGA 4456965 1.5777079 25.38933 30-34
TGTGC 5238560 1.5750589 8.178559 220-229
TCTGG 5222550 1.5702451 11.71216 180-189
CTGGT 5197235 1.5626338 6.852987 160-169
ACTGA 3151340 1.5612477 8.534933 180-189
GCTCA 3939730 1.5609304 10.967918 140-149
GGCCG 6531985 1.5555581 12.747524 120-129
TTGTA 3475695 1.5533623 9.029596 210-219
GTCTG 5132050 1.5430348 8.826738 210-219
GGACG 5172195 1.5401942 7.105598 170-179
CATGT 3657365 1.5390134 9.411574 70-79
GAACC 3288225 1.5338433 11.9991045 70-79
TAGTT 3410680 1.5243059 9.764199 220-229
GTGCT 5040475 1.5155014 7.7968483 220-229
CAAAG 2553885 1.4896368 26.798912 70-79
ACCGT 3746950 1.4845504 22.572578 45-49
CCTCG 4681535 1.4833572 9.391332 130-139
CCCAC 3549045 1.4818394 10.583057 240-247
GTGGA 4652365 1.4714023 25.79868 25-29
AAATA 1686065 1.4618391 5.113216 9
CCACC 3497295 1.4602323 9.368944 230-239
GCTCT 4305080 1.4487579 9.628476 130-139
CGACT 3615015 1.4322776 8.552607 190-199
CCTCA 3185235 1.4124991 7.5415654 130-139
TCGAC 3557295 1.4094086 9.208022 190-199
GTCCA 3546740 1.4052268 19.651228 70-79
CCATG 3535085 1.4006089 9.553266 70-79
TGATA 2647100 1.3928488 7.5316606 180-189
TGGAC 3923985 1.3890399 10.260471 170-179
CTCTT 3471045 1.38855 8.190744 200-209
AAAAA 1343475 1.3713784 8.196002 8
CACTG 3451690 1.3675677 7.288996 230-239
GTAGG 4292435 1.3575674 5.321711 160-169
AGATC 2727510 1.3512723 7.062104 130-139
AATTG 2567575 1.3510044 8.780271 170-179
TATAC 2292705 1.3502415 6.556325 110-119
GGGAG 5038895 1.3406216 6.4208913 150-159
CCATC 2994525 1.3279284 11.337847 240-247
ACTTC 2817010 1.3267593 6.985636 220-229
GGAGG 4972840 1.3230473 19.319485 30-34
CTCGA 3333620 1.320788 8.232382 190-199
GCGTA 3726095 1.3189892 10.238784 210-219
TATGG 3507560 1.3187106 6.1515765 190-199
CTGCT 3915305 1.3175896 5.3995833 90-99
TGGTC 4379140 1.3166602 14.573146 50-59
GAGGG 4915780 1.3078662 7.9342427 200-209
TTGGC 4322500 1.2996305 9.353429 60-69
GTGGT 4836575 1.2992512 12.874651 50-59
CTGAT 3077835 1.295148 7.1745677 180-189
AGCCG 3867535 1.2890345 9.075047 120-129
ACGGA 3089285 1.2875016 9.147719 200-209
CTTGA 3037555 1.2781982 8.3252 200-209
TCCAC 2874120 1.2745346 9.697531 240-247
ACGTG 3584215 1.2687656 9.205131 170-179
GAATT 2394125 1.2597386 9.740274 170-179
GTGTT 3943905 1.2594135 7.3655443 160-169
ATCGG 3553835 1.2580116 8.017109 190-199
TGCCC 3947360 1.250732 8.181857 60-69
AAGTA 2000405 1.2392359 15.291309 70-79
GATCT 2933815 1.2345446 5.616589 130-139
ATAAA 1416615 1.2282227 5.741541 7
ACTAT 2085375 1.2281388 6.537947 230-239
GGCTC 4309350 1.2199432 7.977736 130-139
ACTCG 3064715 1.2142472 8.431042 190-199
GGAGT 3835330 1.2129989 6.7415876 210-219
CCATA 2173620 1.2052853 11.990628 70-79
ATAAC 1734895 1.2029263 7.140375 80-89
ATGGG 3752270 1.1867296 36.528973 15-19
TTGAG 3146740 1.1830559 8.035298 200-209
GCATA 2387645 1.1828952 8.126543 210-219
ATACC 2131280 1.1818076 8.702458 70-79
ACTGG 3331610 1.1793468 6.9651775 160-169
GGAAT 2638700 1.1679839 8.122106 170-179
TATCA 1976290 1.1638955 7.5259075 70-79
AAAGT 1865800 1.1558491 19.505054 70-79
ACTGT 2745985 1.1555061 5.6543994 230-239
AGTTC 2745480 1.1552936 5.585353 160-169
AGCTT 2719105 1.144195 6.076275 150-159
AGAGA 2188505 1.140504 14.915246 35-39
CAATA 1642680 1.1389871 6.7596016 100-109
AAAAG 1558815 1.1369283 15.572114 70-79
TTCCA 2408140 1.1341891 7.5594535 230-239
TCTGT 3163600 1.1307151 6.7239256 210-219
GAAGA 2151075 1.120998 20.000788 35-39
AGAGC 2681435 1.1175245 6.799472 140-149
AGGTT 2922355 1.0986955 22.500168 10-14
ATATC 1863735 1.0976084 8.019968 70-79
GTCTA 2601415 1.0946712 7.4212813 180-189
TCTAC 2315855 1.0907246 7.2341056 180-189
TCACG 2745260 1.0876784 9.173987 200-209
AACTT 1838960 1.0830177 6.309023 200-209
CTGAA 2184280 1.0821434 16.20109 35-39
CTCGG 3768470 1.0668242 7.9569983 130-139
ATCCA 1922185 1.0658631 15.961829 240-247
CGGCT 3753255 1.062517 7.488597 130-139
GTCCG 3712135 1.0508764 5.10456 130-139
CTGCC 3309850 1.0487351 6.0313497 200-209
TACTG 2488375 1.0471041 6.232169 180-189
GAGCT 2941755 1.041343 6.0758624 140-149
GCTTC 3070585 1.033322 5.3595614 150-159
CATGG 2907200 1.0291111 5.4691563 190-199
GCGGT 4057370 1.0262254 5.7245 130-139
CGTGG 4016645 1.0159248 8.852967 50-59
TATGT 2269460 1.0142703 7.367233 220-229
TCCCC 2852590 1.0116413 5.3913884 80-89
ACGTC 2530605 1.0026315 13.551777 70-79
TAGAT 1894935 0.99707526 7.9176855 240-247
CTGCG 3510585 0.9938191 6.638533 210-219
CTTCT 2466480 0.9866858 5.6409044 210-219
ACAAT 1418550 0.9835817 7.842226 100-109
AAAAT 1132005 0.98146224 5.3637047 8
TATAA 1329730 0.97923356 6.630852 240-247
CGAAC 2097110 0.97822934 12.639945 70-79
CCATT 2070870 0.97534126 6.5022373 50-59
AAGAG 1864550 0.9716801 14.987332 35-39
TAGGC 2730175 0.9664466 5.970165 160-169
GAGGC 3237770 0.9641544 16.470333 30-34
GAGTT 2535875 0.9533936 5.3424644 160-169
CTACC 2149820 0.9533422 8.048069 230-239
GGTGG 4216965 0.9529463 25.416199 25-29
TGAAG 2152560 0.95280087 10.726957 35-39
AGCTG 2688830 0.95181084 5.1845684 30-34
TGCGT 3158675 0.9497073 8.059985 210-219
GGGGT 4155745 0.9391119 7.1015162 10-14
CCCAA 1785870 0.932393 9.847143 240-247
CAGCG 2742050 0.9139147 5.874974 160-169
GGGCC 3834590 0.9131877 19.242392 15-19
ATCAA 1315460 0.912102 14.4648 70-79
ACCCA 1746860 0.91202617 12.860369 240-247
TCGAA 1830520 0.90688246 16.681684 70-79
CGGAG 3036495 0.904218 6.3113966 210-219
TGCAT 2140320 0.90064317 6.190933 210-219
TGTAA 1699405 0.89419144 6.6940217 230-239
CGTCC 2817200 0.8926376 11.007637 70-79
GACCG 2640605 0.88010347 20.609962 45-49
TGGGC 3450870 0.87282413 20.899258 15-19
CATCA 1564495 0.8675218 5.747487 230-239
TTCTG 2423485 0.8661875 5.570459 210-219
ACTAA 1234565 0.85601175 12.177471 240-247
GGTCC 3015965 0.85379606 14.613157 50-59
CGGTC 2996985 0.8484229 5.439345 130-139
CCAAT 1525075 0.84566313 10.706273 240-247
CCAGC 2192780 0.8180033 5.4918637 80-89
AGCGT 2250730 0.79672915 6.038572 160-169
TGAAC 1594145 0.7897768 6.0837417 190-199
TAACC 1395780 0.77396846 5.6862373 80-89
GTATG 2052120 0.7715199 5.1018896 220-229
CTTCC 2046625 0.7708724 5.279834 230-239
CATAC 1385090 0.7680407 10.407547 70-79
AAAGA 1028095 0.7498454 5.8429985 8
TGAGA 1691570 0.74875 5.6715918 240-247
AACCG 1598100 0.7454585 5.3969817 190-199
CCTTT 1853475 0.7414605 5.5823407 50-59
AAAGG 1410795 0.73521304 8.697761 10-14
AGCCA 1565365 0.7301887 5.6364307 80-89
GTCGA 2036185 0.7207831 11.427937 70-79
AAAGC 1235570 0.7206865 10.565951 70-79
AATCC 1297265 0.7193413 9.490708 240-247
ATGAT 1357365 0.7142171 7.061755 240-247
GTAGC 2013480 0.71274585 5.419733 80-89
CATTG 1668415 0.7020663 5.939066 50-59
CGATG 1982470 0.70176864 16.174759 15-19
TGTTA 1543170 0.6896756 5.1868453 230-239
CTAAT 1168555 0.6881964 9.402812 240-247
AAGGT 1546910 0.6847182 9.539983 10-14
AGGAT 1535625 0.679723 7.023027 10-14
GGAAG 1795120 0.6684264 7.096199 30-34
TAGGT 1776860 0.6680325 5.32436 10-14
ATCCC 1488745 0.6601871 5.0110326 240-247
CCGTA 1659715 0.65758294 5.5575085 80-89
CCCAT 1462265 0.6484444 5.054259 240-247
GAGGT 2032240 0.6427361 6.582037 10-14
AGAAG 1223535 0.6376255 5.4917397 9
TATGA 1188135 0.62517184 5.9050307 240-247
ATAGG 1409235 0.62377834 5.295968 10-14
CAATC 1120010 0.6210523 9.178913 240-247
CATCC 1317965 0.58445436 7.8031206 240-247
AATGG 1287130 0.5697303 6.5662127 10-14
AAATG 898035 0.556326 5.2152967 9
TAAAA 640910 0.5556769 6.098959 8
AAGCA 944555 0.5509425 7.1739507 70-79
GGGTC 2156810 0.5455192 5.3548446 70-79
AAGAA 737055 0.5375742 5.352573 8
GAAGG 1424025 0.5302464 5.4032836 10-14
ACGAT 1039100 0.51479447 7.200775 10-14
GAAAA 697860 0.5089871 5.96407 8
TTGCC 1506890 0.5071029 5.787562 60-69
AAGGG 1339285 0.49869278 6.023067 10-14
TGATC 1160055 0.4881493 5.481979 240-247
ATAAG 775200 0.48023063 5.746588 9
GGTCG 1879765 0.47544655 5.4524565 70-79
GATCC 1179360 0.4672652 5.481847 240-247
GAAGC 1093880 0.45588937 5.7183084 30-34
TACCG 1135050 0.44970945 5.4812217 70-79
AAGCT 889310 0.440585 9.610767 30-34
TAAGG 986170 0.4365145 5.8005943 10-14
CGGTT 1447110 0.43509728 7.7669168 10-14
TAAAG 641265 0.3972589 5.6772895 9