FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_10_SL248339.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_10_SL248339.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7511701
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 8358740 8.682193 60.952538 110-119
CACAG 11300545 7.522104 41.575172 110-119
ATACA 7652200 7.502308 38.01471 110-119
TCCCT 13379180 7.3135424 108.18886 30-34
CAGTA 9664955 6.8158474 39.98638 60-69
AGTAA 7299100 6.375577 47.377575 110-119
GGAGA 11714260 6.18925 91.00329 35-39
CCCTG 13448370 6.182007 70.24036 30-34
GTAAT 7929720 5.9245863 39.612545 110-119
GGTGA 12985040 5.8683558 99.87716 45-49
AATAC 5587275 5.477831 39.825073 110-119
GTAGT 9661225 5.1921096 25.02963 80-89
ACAGT 7331065 5.169959 35.50926 110-119
AGACG 8685430 5.150779 97.99679 40-44
GTGAC 9946350 5.045397 82.28473 45-49
ACTCC 7862750 5.024847 123.95109 30-34
GAGAC 8190210 4.8570957 136.91974 40-44
ACCAG 7192765 4.787798 94.014435 45-49
CCCCA 7908205 4.7703056 99.170715 60-69
CTCAG 8160510 4.6463 35.11546 140-149
CACTC 7109750 4.5436273 100.368774 25-29
GCACA 6808580 4.532069 33.745686 110-119
GTGTC 10092040 4.3788548 23.863632 120-129
CGTGT 10081590 4.37432 23.32767 120-129
GGCCC 11242985 4.3461328 58.476524 60-69
TGACC 7584050 4.3180842 140.31293 45-49
CTCCC 8298995 4.281971 70.19194 30-34
TAGTA 5712145 4.2677536 31.44333 80-89
ATGCA 5977410 4.2153444 97.27833 25-29
AGTAG 6593915 4.142905 28.517971 70-79
GACCA 6213305 4.13583 105.58429 45-49
CCCAG 7493515 4.027128 84.98436 60-69
TGAGG 8532960 3.856318 67.273735 35-39
CAGGG 8968785 3.8258386 36.106243 50-59
CCACT 5858595 3.7440517 29.638842 240-247
AGGAG 7077265 3.7392857 91.32377 35-39
TACAC 4706785 3.7256565 27.71163 110-119
TGCAC 6516455 3.7102344 117.63118 25-29
ACGGT 7274515 3.690078 93.2507 40-44
ATGGT 6830515 3.670837 26.365572 190-199
CTGAG 7202465 3.65353 103.4585 35-39
GACGG 8557825 3.6505344 104.048645 40-44
GGGTT 9377345 3.6249585 43.320286 50-59
TGTCC 7304545 3.5574036 20.542555 120-129
AGATA 4039330 3.5282512 19.033766 150-159
CCTGA 6155340 3.5046284 84.56487 30-34
GAGGA 6627640 3.5017257 120.05158 35-39
GCACT 6029215 3.4328175 90.80026 25-29
CCAGG 7157190 3.426842 41.10511 50-59
GCCCC 7895595 3.425821 71.38848 60-69
CCCTT 6249250 3.4160662 31.629173 20-24
CTTCA 5022140 3.4003003 17.088177 200-209
GGATG 7504170 3.3913746 70.531715 20-24
TCTTG 6571810 3.3908224 18.678473 170-179
TGGTG 8683980 3.3569274 18.388279 190-199
CTCTC 6001305 3.28053 13.819789 100-109
CCTGG 7963275 3.261318 29.84291 50-59
TCAGG 6388265 3.2405186 21.127947 140-149
CCGTG 7907310 3.2383978 23.48248 120-129
CTTGG 7415985 3.2177358 17.655104 20-24
CAGAT 4548620 3.207744 17.54379 150-159
TTCCC 5843465 3.1942494 51.255352 50-59
TCGCA 5576585 3.1751063 24.11965 100-109
CGGAT 6252645 3.1717231 80.42977 20-24
TGTCT 6140030 3.168039 20.184532 180-189
AGGCT 6236075 3.1633184 21.040293 30-34
GGTTG 8168770 3.157765 75.44191 15-19
GGTTC 7129805 3.0935647 49.03348 50-59
CGCAC 5743160 3.086461 24.722416 100-109
TCTCA 4541000 3.0745387 21.565302 130-139
GATGC 6048765 3.0683033 98.24355 25-29
AGGGT 6696745 3.0264738 50.84889 50-59
ACAGA 3650225 3.0094678 15.167643 140-149
AGTCA 4225100 2.9795935 33.115295 70-79
TGGCC 7236780 2.963786 43.500843 60-69
CCAGT 5195940 2.9583807 38.222084 60-69
GTTGG 7643740 2.9548066 53.796688 15-19
TCAAA 2938430 2.8808715 58.382492 70-79
GACTC 5020325 2.858392 20.846249 190-199
ACCAC 3784685 2.8276663 20.23487 230-239
TCTCT 4858415 2.8136694 15.19366 180-189
GCCGT 6844800 2.8032527 22.243475 120-129
TTCTT 4562785 2.799552 21.312725 170-179
GTTCC 5698905 2.775437 55.65095 50-59
CGGTG 7579310 2.765494 65.8258 40-44
GCCCT 5919195 2.7209623 17.170532 20-24
CGGCC 6910660 2.671412 12.562033 120-129
TCACA 3366120 2.664453 12.213709 140-149
GATGG 5837645 2.6382189 17.570238 15-19
GCGGA 6153210 2.6247914 87.9832 20-24
AGGGA 4937265 2.608613 9.761995 150-159
AGATG 4104665 2.578929 17.014267 180-189
AAAAA 1788240 2.5387182 14.16516 120-129
GGCGG 8216120 2.5209913 42.506615 20-24
TTGGG 6414710 2.4797058 81.03136 15-19
TGTTC 4761505 2.456769 12.942249 140-149
TCCAT 3618625 2.4500334 15.299441 70-79
CTGGC 5977640 2.4481113 35.772923 60-69
TCCTC 4463285 2.4397926 16.784107 130-139
ACAGC 3650015 2.429599 11.18964 120-129
GTTCT 4662315 2.4055903 18.57636 160-169
GGCTG 6577450 2.3999412 11.703762 140-149
ACTCT 3539070 2.39617 14.004305 200-209
CAGCT 4202160 2.3925583 12.466008 150-159
CACTA 3020610 2.3909645 15.8757305 240-247
TGGAG 5238930 2.3676403 14.353642 25-29
GAGAT 3758955 2.3617222 16.56496 180-189
TCAGC 4118530 2.3449423 15.1737585 140-149
TACCA 2929095 2.3185258 16.68934 230-239
CACGG 4753640 2.276029 9.471146 120-129
AATAA 1849700 2.2461562 9.332708 110-119
TGTAG 4177730 2.2451844 8.85845 210-219
TCATT 3128235 2.243923 16.585117 150-159
CTGGA 4421115 2.2426593 14.428649 180-189
CATTT 3123640 2.2406268 17.690264 150-159
CAGAG 3752440 2.2253344 10.155694 140-149
CTACT 3275265 2.2175577 8.01842 180-189
TTCAT 3071580 2.2032835 16.115515 140-149
GAACT 3110265 2.1933982 11.752172 160-169
TGGGG 6710945 2.1815658 53.044224 15-19
TTCAC 3207480 2.171663 18.666122 200-209
TAGTC 3577775 2.1581569 27.50644 70-79
TCTCG 4427270 2.156135 17.416046 100-109
GTGAA 3405100 2.1393976 17.860483 190-199
TCTGC 4382990 2.1345701 12.334476 210-219
GATAC 3024885 2.1331868 14.442782 150-159
ATCTC 3140005 2.125978 9.4210205 100-109
ACTAC 2684195 2.124675 12.343587 230-239
GATAT 2841315 2.122851 8.907554 180-189
TTGGA 3944900 2.1200576 16.602394 170-179
TTGTC 4093025 2.1118567 13.342882 170-179
CTCGC 4581760 2.106164 16.478935 100-109
ATTTG 3280315 2.0963593 15.579482 150-159
GTCAA 2963575 2.0899503 41.743656 70-79
AGCTC 3644365 2.07497 13.085476 140-149
ACACG 3110295 2.0703392 13.205198 120-129
GTCTC 4189895 2.0405307 11.85039 180-189
GTTCA 3349155 2.0202508 15.038798 140-149
ATATA 1942825 2.0180054 9.968429 110-119
TACAG 2850605 2.0102825 13.818818 160-169
ACACA 2171470 2.0094736 13.496834 110-119
GCTGT 4627865 2.0079932 10.0914345 140-149
GAATC 2841400 2.003791 14.296742 190-199
GTCCT 4100155 1.9968262 14.2035 130-139
CTCTG 4082600 1.9882762 14.529928 180-189
CAGGC 4107580 1.9666971 15.160174 140-149
AACCA 2120505 1.9623109 15.276891 70-79
TGAAT 2624055 1.9605283 13.942564 190-199
ATGTA 2618980 1.9567363 7.1279087 150-159
GGGGC 6337220 1.9444793 48.13429 15-19
ACGGC 4056160 1.9420774 14.2014265 120-129
AATAT 1868770 1.941085 12.475461 110-119
TTGCA 3207215 1.9346309 14.316576 150-159
CCTTC 3518285 1.9232216 14.096597 200-209
CAGCC 3560235 1.9133241 10.262342 120-129
ATATG 2546775 1.9027894 9.130882 180-189
AATCG 2692640 1.8988837 13.100954 190-199
GGGCG 6167675 1.8924571 59.869427 20-24
TGGAA 3009040 1.8905562 9.359349 170-179
GAGTC 3699435 1.8765795 12.345552 210-219
TTTGC 3626925 1.8713657 12.164853 150-159
CTCCA 2925480 1.869586 8.185245 150-159
GTCAC 3241520 1.8456045 9.340009 130-139
CATAG 2608690 1.8396809 8.52107 210-219
TCGGC 4490755 1.8391653 7.776064 130-139
TACTA 2189825 1.8364029 9.608028 230-239
AGAAC 2188565 1.8043865 12.012493 160-169
TTCAG 2961595 1.78647 10.036337 150-159
GACGT 3493675 1.7722052 10.481529 170-179
GTTGT 3847020 1.7684226 7.684065 210-219
CTGTT 3415510 1.7622827 12.7240505 140-149
CCAGA 2647345 1.762181 23.806421 60-69
AGTAT 2353605 1.7584649 12.095333 220-229
GGTCA 3381730 1.7154201 8.314394 130-139
TGGTT 3726595 1.7130649 18.193762 10-14
AACTG 2422400 1.708307 11.539986 160-169
ATTGT 2660015 1.6999425 7.581502 170-179
CGTAG 3349625 1.6991346 9.595427 210-219
GTCTG 3897145 1.6909398 9.1938305 210-219
TGCAG 3323205 1.6857327 13.48612 150-159
GCAGA 2842110 1.6854755 14.273943 150-159
AGTCT 2789925 1.6829165 13.214398 210-219
CACCA 2243580 1.6762545 13.723331 230-239
TGACT 2771125 1.6715763 12.302459 190-199
GAGAA 2273315 1.6698264 10.964215 160-169
ATAGT 2195560 1.6403838 7.682318 220-229
ACTGA 2318205 1.6348273 9.526142 180-189
TCCCA 2551780 1.6307657 10.877686 240-247
CTGGT 3723025 1.6153903 7.5976014 160-169
CAAAG 1958830 1.6149786 29.547615 70-79
TTGTA 2508220 1.6029346 10.031341 210-219
TCAGA 2269340 1.6003671 10.042776 130-139
TAGTT 2501770 1.5988123 10.560972 220-229
GCTCC 3422040 1.5730588 6.253361 150-159
CCTTG 3227395 1.5717809 20.200687 20-24
GCTCA 2759000 1.5708753 11.653348 140-149
GTGCT 3610665 1.5666382 8.895809 220-229
AATAG 1792025 1.565288 9.787505 240-247
GAACC 2344970 1.560908 12.575668 70-79
TCTGG 3593705 1.5592794 11.8822975 180-189
CAAAA 1359065 1.5577537 20.127876 70-79
GGACG 3649820 1.5569135 7.7963614 170-179
TGTGC 3585640 1.5557802 8.526985 220-229
ATAGA 1779715 1.5545354 10.564997 240-247
TTGGT 3380150 1.5538089 21.94444 25-29
TGCTC 3187435 1.5523202 5.1316543 150-159
GGCCG 4469205 1.5391936 13.072469 120-129
CTGTG 3528705 1.5310764 5.387015 210-219
GTCCC 3251875 1.4948366 17.81933 50-59
CCCAC 2459120 1.4833649 10.684254 240-247
GCTCT 3036460 1.4787936 9.998283 130-139
CCACC 2451350 1.478678 10.152825 230-239
ACCAT 1840175 1.4565909 10.731217 45-49
CGACT 2531620 1.441413 9.518384 190-199
TGATA 1924415 1.4378012 8.396203 180-189
AGATC 2029915 1.4315217 7.3392196 130-139
CTCTT 2462345 1.4260255 8.784629 200-209
CCTCG 3097250 1.4237577 9.417796 130-139
TGGAC 2801565 1.421125 11.035621 170-179
AGCTT 2355840 1.4210713 6.734562 150-159
TATAC 1694535 1.4210492 6.5286 110-119
CTGCT 2916155 1.4202034 5.9360967 90-99
CTTGA 2347685 1.416152 9.0872555 200-209
TCGAC 2479010 1.4114588 10.276731 190-199
AATTG 1871165 1.3980162 9.345094 170-179
CACTG 2454645 1.3975863 7.872196 230-239
GGGAG 3676920 1.3973938 7.0403624 150-159
GAGGG 3669095 1.39442 8.9409685 200-209
AAATA 1136335 1.3798919 5.37222 9
CATGT 2271410 1.3701421 6.738979 150-159
CAGAC 2057725 1.3697058 13.565132 60-69
GGAAT 2160165 1.3572147 8.847339 170-179
CTGAT 2243450 1.3532761 7.915078 180-189
GCTGA 2644570 1.3414875 18.057152 30-34
ATCGG 2628925 1.3335514 7.870724 190-199
CTCGA 2321000 1.3214936 9.1893215 190-199
CCATC 2065155 1.3197784 11.1784315 240-247
GCGTA 2587735 1.3126574 10.044967 210-219
CCTCA 2051600 1.3111156 7.275368 130-139
GAATT 1746270 1.3047028 10.419662 170-179
AGAGC 2193430 1.3007845 7.894395 140-149
ACGTG 2556255 1.2966888 10.042077 170-179
TATGG 2412525 1.2965326 6.492516 180-189
TCCAC 2023760 1.293324 10.608719 240-247
AAAGT 1473005 1.2866321 22.065245 70-79
ACGGA 2162580 1.2824892 9.223204 200-209
ACTTC 1877630 1.2712718 6.6526947 220-229
AGCCG 2639990 1.2640196 8.959313 120-129
ACTAT 1503360 1.2607285 6.6547217 230-239
ACTCG 2199175 1.2521309 9.33521 190-199
AAGTA 1423580 1.2434607 15.913296 70-79
CCATG 2178275 1.2402312 6.777134 70-79
GTGTT 2696555 1.2395695 7.5403743 160-169
ACTGG 2441775 1.2386175 7.719092 160-169
GGCTC 3005370 1.2308338 8.242918 130-139
GGAGT 2713760 1.2264351 7.1824183 210-219
GATCT 2021530 1.2194114 5.444503 130-139
TTGAG 2264255 1.2168498 8.719043 200-209
GTGGA 2691425 1.2163413 18.72233 25-29
ATAAC 1236795 1.2125685 7.719853 80-89
GAAGA 1634360 1.2004923 17.476536 35-39
ATATC 1430405 1.1995479 6.998083 70-79
TTCCA 1738570 1.1771197 8.136646 230-239
CTGAA 1658425 1.1695422 13.554543 35-39
ACCGT 2053360 1.1691091 15.906645 45-49
GGAGG 3064400 1.1646088 13.8610325 30-34
GCATA 1647905 1.1621233 8.2397785 210-219
GTCCA 2021855 1.1511714 14.331044 70-79
TCTAC 1687615 1.14262 7.7371297 180-189
ATCCA 1439425 1.1393771 16.524956 240-247
TTGGC 2620925 1.1371982 7.3307657 60-69
GTCTA 1883125 1.1359239 7.8000617 180-189
ATAAA 934300 1.1345536 5.754078 7
GCTTC 2327970 1.1337502 5.955903 150-159
TGGTC 2602160 1.1290562 10.509146 50-59
TATCA 1342635 1.1259433 6.5265527 70-79
GTGGT 2909255 1.1246177 9.223583 50-59
ACTGT 1863705 1.1242095 5.9679627 230-239
AGGTT 2088265 1.1222697 23.107538 10-14
TACTG 1856440 1.119827 7.0561314 180-189
ATACC 1412800 1.1183022 6.6535873 240-247
GAGCT 2176970 1.1042923 6.867611 140-149
AGTTC 1829230 1.1034136 5.403816 160-169
TCACG 1927910 1.0976824 9.386162 200-209
AGTTG 2034520 1.0933863 5.164887 220-229
TGCCC 2377900 1.0930839 5.854686 60-69
CAATA 1104070 1.0824432 6.3920336 100-109
TCTGT 2086320 1.0764676 6.562184 210-219
CTTCT 1848210 1.0703598 5.7661033 210-219
CGGCT 2613050 1.0701611 7.697433 130-139
CTCGG 2579805 1.0565457 8.293592 130-139
CCATA 1332675 1.0548792 8.986123 70-79
TAGAT 1400245 1.0461745 8.354109 240-247
AAGAG 1421585 1.044202 13.024785 35-39
AAAAG 1021095 1.0427167 13.127412 70-79
GCGGT 2851785 1.0405425 6.1551394 130-139
TATGT 1625770 1.0389849 7.6989207 220-229
GGGGT 3189935 1.0369706 7.801234 10-14
GTCCG 2530510 1.0363574 5.1450047 130-139
CTGCG 2516800 1.0307424 6.801338 210-219
CTACC 1597445 1.020879 8.857296 230-239
CTGCC 2205225 1.0137078 5.900193 200-209
CATGG 1997910 1.0134621 5.397447 190-199
AGAGA 1374215 1.009407 11.876647 35-39
ATACG 1428140 1.0071424 5.046984 120-129
ACCCA 1344355 1.0044131 13.693876 240-247
CAGCG 2077685 0.99478954 6.3608413 160-169
TGAAC 1389240 0.9797096 6.4920344 190-199
CGAAC 1463540 0.97419196 13.279286 70-79
AACTT 1159865 0.97267115 5.910881 200-209
ATGGG 2149030 0.97121555 27.279087 15-19
TGCTA 1610030 0.9711896 5.1139464 220-229
AGCTG 1909820 0.9687774 5.1178517 80-89
AAAAT 791200 0.9607822 5.049036 8
TATAA 921675 0.9573405 6.7430186 240-247
AGAAA 928300 0.9479568 5.0374436 6
TGCGT 2181980 0.9467434 8.105828 210-219
TTCTG 1817065 0.9375416 5.7692614 210-219
TGTAA 1232280 0.9206817 7.252864 230-239
ACGTC 1605055 0.9138604 9.714894 70-79
CGGAG 2138905 0.91239846 6.7004104 210-219
TAGGC 1794100 0.9100772 5.7074804 160-169
CCAGC 1693020 0.9098546 5.8867316 80-89
CCCAA 1216535 0.90891445 10.861045 240-247
TCCAG 1588060 0.90418404 5.323176 240-247
TCGAA 1281895 0.90400845 16.722612 70-79
GTATG 1679850 0.9027805 5.399422 220-229
ACAAT 920605 0.90257204 7.2873664 100-109
GAGTT 1670025 0.89750034 5.100469 160-169
CCATT 1314820 0.8902147 5.480324 50-59
ACTAA 895725 0.8781794 12.487206 240-247
CGGTC 2133205 0.8736431 6.00562 130-139
TGCAT 1447310 0.87303495 6.266834 210-219
CCAAT 1100170 0.8708398 11.590279 240-247
AGCGT 1695590 0.86010677 6.525297 160-169
TGAAG 1364265 0.85715705 8.886601 35-39
CGTGG 2335395 0.85212517 6.3531713 50-59
CTTCC 1551700 0.84821534 5.7292643 230-239
CATCA 1056630 0.8363757 5.7559743 230-239
ATCAA 812795 0.7968738 11.9049835 70-79
GAGGC 1860680 0.79371536 11.781857 30-34
GACCG 1648080 0.78909594 14.705484 45-49
GGTGG 2425340 0.78841937 18.839018 25-29
TAACC 988005 0.7820557 6.1762776 80-89
AGACC 1166855 0.7767064 5.2022204 240-247
AAAGG 1036985 0.76170033 8.726001 10-14
GGAAG 1430680 0.7559023 6.3354 30-34
GGTGC 2070390 0.7554317 5.333061 220-229
AATCC 953990 0.75513107 10.420384 240-247
AACCG 1134050 0.75487006 5.5672455 190-199
TGAGA 1199530 0.7536553 5.679738 240-247
GGGCC 2174925 0.7490439 14.143174 15-19
AGCCA 1124390 0.74843997 5.9325414 80-89
AAAGA 728965 0.74440086 5.989559 8
GTCGA 1446115 0.73355794 12.078583 70-79
AAGGT 1162560 0.7304273 10.104998 10-14
ATGAT 970815 0.7253316 7.367267 240-247
CTAAT 841695 0.70585144 9.591499 240-247
CGTCC 1535335 0.7057697 7.9832096 70-79
TGGGC 1911335 0.6973967 15.450108 15-19
GTAGC 1365460 0.6926448 5.363473 80-89
GGTCC 1656755 0.67851543 10.626149 50-59
CATAC 827035 0.6546397 7.6935406 70-79
GAAAA 631470 0.6448414 5.4404044 8
CAATC 812745 0.6433285 10.112898 240-247
TATGA 859690 0.642306 6.1169467 240-247
AAAGC 777995 0.6414265 8.966424 70-79
TAGGT 1173915 0.6308822 5.312372 10-14
AGGAT 979350 0.615318 5.192487 10-14
GAGGT 1346495 0.60852426 6.7167573 10-14
GGGTC 1650140 0.6020934 5.8480864 70-79
ATAGG 949170 0.5963561 5.0070353 10-14
AGAAG 809955 0.5949392 5.303001 9
AATGG 905215 0.56873953 6.527197 10-14
CGATG 1106200 0.56113225 12.039106 15-19
CATCC 871930 0.5572242 7.463423 240-247
TAAAA 444935 0.5403003 6.1467214 6
GAAGG 1021885 0.5399147 5.6315355 10-14
AAGAA 510675 0.52148855 5.1774955 9
AAGGG 979880 0.51772135 6.3513336 10-14
GGTCG 1373070 0.5009977 5.955558 70-79
AAGCA 596290 0.49161783 5.933739 70-79
TGATC 810615 0.48897275 5.723475 240-247
GATCC 836775 0.4764295 5.7453313 240-247
ATAAG 528230 0.46139535 5.3980803 9
CGGTT 1044105 0.4530287 8.357492 10-14
TAAGG 692740 0.43524316 5.818497 10-14
ACGAT 612790 0.4321472 5.503852 10-14
TAAAG 481125 0.42025036 5.327272 9
AAGCT 560795 0.3954797 7.876549 30-34
TGGGT 996295 0.38513333 5.0944757 10-14