FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_15_SL248344.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_15_SL248344.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9845937
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10981235 8.665361 60.402775 110-119
ATACA 10048275 7.4965754 36.539543 110-119
CACAG 14672765 7.4593096 41.68528 110-119
TCCCT 17271615 7.1998687 105.092766 30-34
CAGTA 12422845 6.679929 38.80517 110-119
AGTAA 9575960 6.3696322 46.94149 110-119
GGAGA 15379295 6.215045 91.60637 35-39
CCCTG 17116605 6.0145864 68.346695 30-34
GTAAT 10410035 5.9206185 39.155746 110-119
GGTGA 17023520 5.8822036 100.228096 45-49
AATAC 7242575 5.403366 38.597607 110-119
AGACG 11729730 5.3166156 99.63754 40-44
GTAGT 12818610 5.2545447 28.232222 80-89
ACAGT 9670040 5.199709 35.25678 110-119
GTGAC 13012830 5.0431495 83.050095 45-49
GAGAC 10822940 4.905604 139.32819 40-44
ACTCC 9877230 4.815529 119.59406 30-34
CCCCA 10444065 4.8140907 100.51317 60-69
CTCAG 10749410 4.672551 35.024567 140-149
ACCAG 9126895 4.6399117 90.89295 45-49
TAGTA 7961450 4.528006 36.001225 80-89
GGCCC 15165420 4.4919934 58.493145 60-69
GCACA 8825570 4.4867244 33.9569 110-119
CACTC 8933605 4.355476 96.53558 25-29
TGACC 10019170 4.355131 141.82959 45-49
GTGTC 13137760 4.353456 23.643171 120-129
CGTGT 13111010 4.3445916 23.125607 120-129
AGTAG 8805410 4.221443 29.034918 80-89
CTCCC 10654155 4.1990066 68.52864 30-34
ATGCA 7551895 4.0607543 93.926094 25-29
GACCA 7975380 4.0545073 103.252914 45-49
CCCAG 9807185 4.0304146 85.632545 60-69
TGAGG 11068405 3.8245094 67.86883 35-39
AGGAG 9291300 3.7547786 91.878395 35-39
ACGGT 9581400 3.7132912 94.61723 40-44
TACAC 6122800 3.692668 26.709845 110-119
CAGGG 11298095 3.6908958 35.02194 50-59
GACGG 11286210 3.6870131 106.05011 40-44
CTGAG 9487160 3.6767683 103.989555 35-39
CCCTT 8820110 3.6767626 36.292652 20-24
ATGGT 8911285 3.652872 26.212297 190-199
TGTCC 9755745 3.625874 20.494629 120-129
CCACT 7405830 3.6106267 28.52974 240-247
TGCAC 8255655 3.5885663 113.247055 25-29
AGATA 5385735 3.5824244 19.470766 150-159
GAGGA 8736555 3.5305963 120.95108 35-39
GCCCC 10489870 3.4849308 72.44919 60-69
GGGTT 11724470 3.4639108 41.643753 50-59
CCTGA 7848280 3.4114888 82.36254 30-34
TGGTG 11496595 3.3965864 18.213526 190-199
CTTCA 6516915 3.3605857 17.440226 200-209
CTTGG 10123610 3.3546581 20.586304 20-24
GGATG 9704290 3.3531613 68.0353 20-24
CCGTG 10700080 3.3522453 23.374153 120-129
CCAGG 9082420 3.3278792 39.846 50-59
TCTTG 8440070 3.3178933 18.50684 170-179
CTCTC 7958260 3.3174908 13.835978 130-139
AGGCT 8540350 3.3098304 24.90043 30-34
GCACT 7609455 3.3076763 87.35232 25-29
CAGAT 6065315 3.2614005 18.039568 150-159
TCAGG 8340050 3.2322037 21.032383 140-149
CCTGG 10169160 3.1859126 28.91529 50-59
TCGCA 7278835 3.1639621 23.64615 100-109
TGTCT 7990985 3.1413527 20.261452 180-189
TCTCA 6082225 3.1364286 21.784313 130-139
CGCAC 7480240 3.0741205 24.130793 100-109
CGGAT 7909920 3.065506 77.55298 20-24
TTCCC 7315195 3.0494223 49.28879 50-59
GGTTG 10280455 3.0372865 72.8326 15-19
ACAGA 4752285 2.9886181 14.183627 140-149
TGGCC 9471740 2.9674168 43.26332 60-69
GGTTC 8952580 2.966614 47.164776 50-59
GATGC 7641895 2.9616325 94.87525 25-29
AGGGT 8469120 2.9263682 48.893852 50-59
TCTCT 6527630 2.8781357 15.641545 180-189
GCCCT 8189375 2.8776562 19.495499 20-24
AGTCA 5334615 2.8684933 31.611544 70-79
TCACA 4717725 2.8452652 11.767039 140-149
GTTGG 9573140 2.8283157 52.045475 15-19
TCAAA 3784920 2.8237624 57.01595 70-79
CCAGT 6476820 2.8153427 36.58052 60-69
GCCGT 8979045 2.813059 22.093382 120-129
ACCAC 4920745 2.8058012 20.033495 230-239
CGGTG 10043080 2.8052807 66.93928 40-44
GACTC 6422735 2.7918332 19.447126 190-199
GATGG 8033345 2.775793 19.80305 15-19
TTCTT 5942000 2.771102 21.072815 170-179
CGGCC 9154630 2.7115989 12.515741 120-129
GTTCC 7274270 2.7035954 53.583176 50-59
AGATG 5468360 2.6216123 17.36414 180-189
TCCAT 4971785 2.5638068 17.867674 70-79
GCGGA 7758075 2.5344315 85.1113 20-24
TCCTC 6067105 2.5291414 17.349413 130-139
GGCTG 9035285 2.5237787 13.01526 30-34
TGTTC 6395810 2.51427 13.237439 140-149
TGGAG 7254085 2.5065324 16.983076 25-29
AGGGA 6169990 2.493402 8.99131 150-159
ACAGC 4826785 2.453831 11.163171 120-129
GGCGG 10356720 2.4385302 41.357765 20-24
GAGAT 5037080 2.4148502 16.90541 180-189
TTGGG 8097925 2.392474 78.06135 15-19
GTTCT 6051370 2.3788664 17.880182 160-169
ACTCT 4609390 2.37693 13.857103 200-209
CTGGC 7563125 2.3694637 34.634575 60-69
AAAAA 2187275 2.3608768 13.715962 120-129
CAGCT 5337285 2.32001 12.273962 150-159
AATAA 2510365 2.3168054 9.927065 110-119
CTGGA 5965185 2.3118196 14.924622 180-189
CATTT 4220725 2.3020992 18.186686 150-159
CACGG 6251755 2.2906985 9.456598 120-129
TCATT 4195785 2.2884965 16.858828 150-159
TACCA 3784805 2.2826202 16.710466 230-239
TCAGC 5199135 2.259959 14.377869 140-149
TTCAT 4132105 2.253764 16.343733 140-149
TGTAG 5480110 2.246381 8.095132 210-219
TTCAC 4326325 2.2309618 19.222525 200-209
GTCAA 4118340 2.2144861 41.886604 70-79
GTGAA 4566410 2.1892042 18.237022 190-199
CAGAG 4827315 2.188028 9.295678 140-149
TTGTC 5541915 2.1785936 13.61288 170-179
GATAC 4004830 2.1534503 14.641197 150-159
ATTTG 4418485 2.1486764 15.904695 150-159
TCTGC 5749275 2.1368072 12.439324 210-219
CTACT 4138920 2.1343222 7.6727805 230-239
ACTAC 3523745 2.1251745 12.672932 230-239
GAACT 3946900 2.1223006 10.957011 160-169
ATCTC 4109010 2.1188984 9.379212 100-109
TCTCG 5693170 2.1159546 16.797518 100-109
TGGGG 8456410 2.1059885 51.155663 15-19
CTCGC 5951450 2.0912743 16.08096 100-109
CACTA 3463555 2.0888739 15.622466 240-247
GTCTC 5611160 2.0854747 12.319301 180-189
TAGTC 4535685 2.0853379 26.03799 70-79
GTCCT 5575790 2.0723288 14.641414 130-139
TTGGA 5052485 2.071091 15.918888 170-179
CTCTG 5532130 2.0561018 14.979307 180-189
TACAG 3818685 2.0533578 13.8920965 160-169
GTTCA 4464850 2.0527706 15.315682 140-149
GCTGT 6186335 2.0499642 10.292391 140-149
GAATC 3809830 2.0485961 14.800477 190-199
GATAT 3597475 2.0460331 8.187286 180-189
ATATA 2583350 2.0385377 10.550745 110-119
ACACA 2872350 2.0260246 13.743215 110-119
CAGGC 5505510 2.0172677 15.44293 140-149
AGCTC 4639770 2.0168142 12.125035 140-149
ACACG 3962105 2.0142467 13.195929 120-129
ATGTA 3539445 2.0130289 7.907425 70-79
TTGCA 4357800 2.0035532 14.650094 150-159
TGAAT 3515870 1.999621 14.201644 190-199
AATAT 2523925 1.991645 13.1312895 110-119
AATCG 3675665 1.9764535 13.668728 190-199
TTTGC 5025145 1.9754452 12.504187 150-159
CCTTC 4683205 1.9522471 14.468551 200-209
TGGAA 4055065 1.944058 9.353005 170-179
ACGGC 5287345 1.9373301 14.238034 120-129
AACCA 2742280 1.9342791 14.63478 70-79
GAGTC 4929225 1.9103315 12.738652 210-219
TCGGC 6055665 1.897189 7.9200864 130-139
CAGCC 4608015 1.8937352 10.18078 120-129
GGGGC 7981765 1.8793378 46.592804 15-19
CCAGA 3683315 1.8725158 26.839485 60-69
ATATG 3284955 1.86829 8.376038 180-189
CATAG 3425405 1.8418858 8.4982815 210-219
TACTA 2887010 1.8416306 10.193769 230-239
GGGCG 7777100 1.8311486 58.094902 20-24
CTGTT 4577155 1.7993346 13.00274 140-149
GACGT 4642600 1.7992493 9.755079 170-179
CTCCA 3680535 1.7944022 7.9853206 150-159
GTCAC 4085125 1.7757213 8.609481 140-149
AGTAT 3111910 1.7698721 12.181582 220-229
GTTGT 5018645 1.7589903 7.1069336 210-219
CGTAG 4521290 1.7522352 9.822294 210-219
GCAGA 3849550 1.7448463 14.6973295 150-159
CCTTG 4688065 1.742392 23.495007 20-24
ATTGT 3571250 1.7366724 7.7045507 170-179
TGCAG 4479025 1.7358555 13.753911 150-159
AGAAC 2745655 1.7266883 11.433709 160-169
TTCAG 3703725 1.702834 9.353469 150-159
AGTCT 3675755 1.6899744 13.447351 210-219
CACCA 2953670 1.6841781 13.75616 230-239
GTCTG 5035725 1.668687 9.291082 210-219
GGTCA 4273015 1.6560159 7.5073266 130-139
ATAGT 2892170 1.6448969 7.581217 220-229
TGGTT 4675440 1.6386998 17.628338 10-14
TGACT 3544630 1.6296881 11.702794 190-199
TCAGA 3024830 1.6264913 10.014741 130-139
AACTG 3024400 1.62626 10.7417145 160-169
CAAAA 1845355 1.6101583 20.810747 70-79
TTGGT 4568545 1.6012342 25.090128 25-29
GAGAA 2855035 1.6008108 10.504306 160-169
TCTGG 4826995 1.5995202 12.226777 180-189
GTCCC 4547585 1.5979714 20.3492 50-59
TCCCA 3277485 1.5978999 11.0117855 240-247
CATGT 3461615 1.5915207 8.896061 70-79
AATAG 2361200 1.570597 9.721478 240-247
GCTCC 4464100 1.5686356 6.356586 150-159
CAAAG 2491860 1.5670816 28.538816 70-79
ACTGA 2903085 1.5610274 8.705129 180-189
TGCTC 4193440 1.558557 5.414073 150-159
GAACC 3059290 1.5552754 12.105762 70-79
CTGTG 4689065 1.5538142 5.4157195 210-219
TTGTA 3174190 1.5435851 9.213209 210-219
CTGGT 4653465 1.5420175 6.995478 160-169
GGCCG 5836195 1.5412571 12.898895 120-129
TGTGC 4638365 1.5370139 8.338105 220-229
ATAGA 2307090 1.5346049 10.218948 240-247
TAGTT 3152595 1.5330836 10.061345 220-229
GCTCA 3507420 1.5246043 10.908753 140-149
GTGCT 4589895 1.5209525 8.144063 220-229
CAGAC 2959435 1.5045114 15.9308405 60-69
ACCAT 2486625 1.4996864 11.426356 45-49
GGACG 4573030 1.4939313 7.216832 170-179
CCTCG 4233570 1.48763 9.729571 130-139
GCTCT 4000460 1.4868329 10.106599 130-139
GCTGA 3743695 1.4508767 21.4376 30-34
CCACC 3144315 1.4493418 9.89666 230-239
CCCAC 3130810 1.4431167 10.567787 240-247
CGACT 3262195 1.4180102 8.6977005 190-199
AATTG 2480605 1.4108226 9.452656 170-179
AGATC 2619400 1.4084861 6.9700747 130-139
TATAC 2194810 1.4000745 6.55862 110-119
CTGCT 3743775 1.3914319 5.645878 90-99
TCGAC 3182110 1.3831989 9.334365 190-199
CACTG 3160950 1.374001 7.4786153 230-239
CTCTT 3115745 1.3737814 8.286406 200-209
TGATA 2409370 1.3703085 7.5879493 180-189
TGGAC 3533500 1.3694153 10.366248 170-179
CCTCA 2791715 1.3610684 7.5232277 130-139
GCGTA 3490905 1.3529075 10.3834305 210-219
GTGGA 3892505 1.3449925 21.769646 25-29
ATCGG 3460915 1.3412849 8.146096 190-199
GGGAG 4603630 1.3408729 6.4245677 150-159
GGAAT 2792815 1.3389167 8.803679 170-179
CTTGA 2909915 1.3378699 8.413459 200-209
GAGGG 4587090 1.3360553 8.142149 200-209
ACCGT 3046130 1.3240911 18.876232 45-49
GAATT 2309395 1.3134485 10.528087 170-179
CCATC 2686835 1.3099353 11.141854 240-247
ACGGA 2886545 1.3083549 9.382709 200-209
GTAGG 3775500 1.3045633 5.181836 160-169
CCATG 2984745 1.2974083 7.9815807 70-79
CTGAT 2819540 1.2963188 7.3621316 180-189
CTCGA 2980065 1.295374 8.357797 190-199
ACTTC 2511850 1.2952888 6.95625 220-229
AGCCG 3502960 1.2835156 9.087097 120-129
TATGG 3112830 1.2759967 6.0989375 180-189
GTGTT 3611295 1.2657266 7.6460943 160-169
GTCCA 2909400 1.2646574 16.639738 70-79
TCCAC 2578430 1.2570838 9.715388 240-247
GGAGT 3620770 1.2510989 7.2425003 210-219
GGCTC 3967005 1.2428294 8.391197 130-139
ACGTG 3196575 1.2388391 9.311496 170-179
AAGTA 1852810 1.2324319 15.280728 70-79
GGAGG 4218925 1.228822 16.435505 30-34
AAAGT 1847210 1.2287071 20.963875 70-79
ACTAT 1922000 1.2260482 6.3655486 230-239
ATAAC 1640025 1.2235506 7.522547 80-89
GATCT 2648675 1.2177615 5.4669023 130-139
TTGGC 3659875 1.2127719 8.198653 60-69
AGAGC 2670645 1.2104962 6.948178 140-149
GTGGT 4075220 1.2039946 10.833829 50-59
ACTCG 2759915 1.1996793 8.514701 190-199
GAAGA 2138450 1.1990234 18.67809 35-39
TGGTC 3614985 1.1978966 12.300511 50-59
ATATC 1852595 1.1817747 7.31213 70-79
ACTGG 3021140 1.170849 7.1595526 160-169
TTGAG 2834975 1.1620997 8.090733 200-209
ATAAA 1256590 1.1597018 5.395861 7
TTCCA 2246055 1.1582261 8.000182 230-239
GCATA 2143450 1.1525615 8.144432 210-219
TGCCC 3274895 1.1507622 6.84885 60-69
AGCTT 2492525 1.1459696 6.1449313 150-159
CTGAA 2128025 1.1442673 14.444491 35-39
ATACC 1892630 1.1414473 7.5095763 70-79
TATCA 1776460 1.133208 6.851071 70-79
TCACG 2601375 1.1307651 9.665998 200-209
AGTTC 2450665 1.1267239 5.592985 160-169
ACTGT 2448530 1.1257422 5.7666664 230-239
CCATA 1856975 1.1199437 10.404065 70-79
AGGTT 2728070 1.1182777 22.682838 10-14
CAATA 1476145 1.1012868 6.44312 100-109
TCTGT 2772515 1.0899091 6.6789303 210-219
CTCGG 3465895 1.0858358 8.38059 130-139
CGGCT 3465560 1.0857309 7.8640037 130-139
TCTAC 2098140 1.0819505 7.2876277 180-189
GTCTA 2348600 1.0797982 7.4380774 180-189
AAAAG 1382895 1.0758171 13.855299 70-79
ATGGG 3099195 1.070877 31.072407 15-19
CTTCT 2384355 1.0512999 5.695674 210-219
GCTTC 2827495 1.0508823 5.486573 150-159
TACTG 2281265 1.0488402 6.3711452 180-189
GAGCT 2686985 1.0413467 6.1564007 140-149
AAGAG 1855230 1.0402226 13.931378 35-39
ATCCA 1721490 1.0382326 15.587754 240-247
AGAGA 1850550 1.0375986 12.954354 35-39
TATGT 2130285 1.0359418 7.5749373 220-229
GTCCG 3299735 1.0337793 5.126788 130-139
CTGCG 3286885 1.0297534 7.011517 210-219
CTGCC 2897365 1.0181022 5.9795346 200-209
GCGGT 3630925 1.0142071 5.713775 130-139
AACTT 1580735 1.0083545 6.0614314 210-219
TAGAT 1772095 1.0078639 7.945176 240-247
CATGG 2573760 0.99746597 5.35616 190-199
GGGGT 3997355 0.99550325 7.6089945 10-14
CTACC 2037225 0.9932255 8.658329 230-239
ACGTC 2277240 0.9898702 11.432202 70-79
ATACG 1835195 0.9868087 5.161597 120-129
CAGCG 2688205 0.98498213 6.326872 160-169
TGCGT 2930780 0.97117186 8.385026 210-219
CGAAC 1892615 0.9621636 12.695957 70-79
TGAAC 1781710 0.9580492 6.589562 190-199
TCCCC 2424580 0.95557344 5.0301595 80-89
TATAA 1206475 0.95203704 6.639899 240-247
ACAAT 1259635 0.9397583 7.4782996 100-109
CGGAG 2869495 0.9374153 6.74036 210-219
CGTGG 3333820 0.9312183 7.495961 50-59
TAGGC 2395220 0.92827255 5.8205633 160-169
CCATT 1788190 0.92211825 5.785863 50-59
TTCTG 2342555 0.92088664 5.6852403 210-219
GAGTT 2244520 0.92006314 5.3129106 160-169
ACCCA 1598775 0.91161907 13.100695 240-247
TGTAA 1583080 0.90036315 6.826026 230-239
CCCAA 1559980 0.8894981 9.943399 240-247
TCGAA 1649975 0.88721347 16.177073 70-79
TGAAG 1837385 0.8808695 9.436695 35-39
GTATG 2138980 0.87680066 5.291299 220-229
GGTGG 3506340 0.8732206 21.681236 25-29
GAGGC 2671425 0.8727092 13.781339 30-34
TGCAT 1897525 0.8724109 6.2187114 210-219
ACTAA 1164890 0.86907315 12.624391 240-247
AGCGT 2222280 0.86124927 6.5129743 160-169
GACCG 2343200 0.85856926 17.409142 45-49
CATCA 1419955 0.85637647 5.970285 230-239
CCAGC 2070415 0.8508691 5.5165577 80-89
CGGTC 2669345 0.8362834 5.4661775 130-139
GGGCC 3142615 0.8299205 16.51303 15-19
CCAAT 1373350 0.82826895 10.788844 240-247
ATCAA 1107550 0.82629436 12.63507 70-79
CAATC 1365560 0.8235708 9.299706 240-247
CTTCC 1926570 0.80311257 5.694613 230-239
CGTCC 2277015 0.80011797 9.415867 70-79
TAACC 1312635 0.79165167 6.0880623 80-89
AACCG 1537100 0.7814277 5.8156886 190-199
TGGGC 2790700 0.7795115 17.886065 15-19
GGTCC 2429570 0.7611639 12.438775 50-59
TGAGA 1585155 0.7599467 5.7232723 240-247
GGAAG 1852465 0.74861383 6.5929027 30-34
AAAGA 961615 0.74808425 5.5723076 8
AAAGG 1332320 0.7470284 8.628888 10-14
AGCCA 1448760 0.73651767 5.593472 80-89
AGACC 1448420 0.73634475 5.0451946 240-247
GTCGA 1860295 0.7209612 11.579795 70-79
CATAC 1178700 0.7108753 8.991756 70-79
CTAAT 1108715 0.70725197 9.909225 240-247
GTAGC 1809060 0.701105 5.422598 80-89
ATGAT 1231740 0.70054156 7.091198 240-247
AATCC 1157925 0.6983458 9.603945 240-247
AAGGT 1456005 0.69803023 9.603247 10-14
AAAGC 1060470 0.6669087 9.488026 70-79
CATTG 1426975 0.6560696 5.275672 50-59
TAGGT 1563185 0.6407735 5.4304338 10-14
AGGAT 1328205 0.636761 5.9966745 10-14
GAAAA 814550 0.63367563 5.604748 8
CGATG 1607435 0.62296474 13.885479 15-19
GAGGT 1792365 0.6193229 6.626622 10-14
AGAAG 1097795 0.61553085 5.2371593 9
TATGA 1077895 0.6130435 5.8984194 240-247
ATAGG 1272695 0.6101487 5.20104 10-14
CCGTA 1389445 0.60396355 5.0282097 80-89
TCAAT 934430 0.5960751 5.8233643 70-79
GGGTC 2099830 0.58653444 5.538181 70-79
AATGG 1198305 0.574485 6.532428 10-14
AAATG 834600 0.5551501 5.2318916 9
CATCC 1116155 0.5441684 7.286582 240-247
TAAAA 587690 0.5423767 6.124558 8
GAAGG 1334510 0.5392991 5.4405794 10-14
AAGAA 674280 0.52455324 5.1941466 8
AAGCA 820910 0.5162541 6.3715143 70-79
AAGGG 1263220 0.5104895 6.1434126 10-14
GGTCG 1741065 0.48632252 5.6463633 70-79
TGATC 1033595 0.47520825 5.4913874 240-247
ACGAT 861960 0.46348733 6.1520224 10-14
GATCC 1062455 0.46182773 5.525205 240-247
ATAAG 690630 0.45938572 5.708166 9
CGGTT 1323660 0.4386209 7.8138037 10-14
TAAGG 899170 0.43107533 5.7570133 10-14
GAAGC 938630 0.4254433 5.133403 30-34
AAGCT 769820 0.41394243 8.416366 30-34
TAAAG 590590 0.3928422 5.197023 9