FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10968607
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12535320 8.945863 62.41884 110-119
ATACA 11489185 7.7328324 37.462734 110-119
CACAG 16860430 7.657105 42.335857 110-119
TCCCT 19486195 7.111225 103.52181 30-34
CAGTA 14011160 6.7469497 39.656727 110-119
AGTAA 10926840 6.5798764 48.371117 110-119
GGAGA 17395310 6.323765 95.37066 35-39
GTAAT 11819380 6.034836 40.143845 110-119
GGTGA 19352615 5.96528 102.732895 45-49
CCCTG 19156265 5.8988132 67.64185 30-34
AATAC 8300380 5.586597 39.4865 110-119
AGACG 13576900 5.516586 102.8999 40-44
ACAGT 11238825 5.411957 36.110207 110-119
GTAGT 14627435 5.343465 30.606117 80-89
GTGAC 14845600 5.11464 85.32599 45-49
GAGAC 12039165 4.891771 143.04068 40-44
CCCCA 12030355 4.883271 102.5249 60-69
TAGTA 9332865 4.7652507 39.68994 80-89
CTCAG 12260125 4.7210484 34.750202 140-149
ACTCC 10770840 4.635743 117.27305 30-34
ACCAG 10025290 4.5529504 92.334045 45-49
GGCCC 17514810 4.550882 58.97393 60-69
GCACA 9771805 4.437831 34.55352 110-119
TGACC 11470575 4.4170136 146.55235 45-49
GTGTC 15062875 4.4002047 23.827011 120-129
CGTGT 14952005 4.367817 23.30173 120-129
AGTAG 10103450 4.352882 31.969902 80-89
CACTC 9936430 4.2766147 95.80866 25-29
CTCCC 11981575 4.1237645 66.96712 30-34
CCCAG 11232005 4.079092 87.577286 60-69
GACCA 8884980 4.0350823 105.87235 45-49
ATGCA 8342285 4.0171533 93.90285 25-29
TGAGG 12414175 3.8265643 69.39476 35-39
CCCTT 10468290 3.8202624 40.131294 20-24
AGGAG 10484385 3.8114173 95.66719 35-39
ACGGT 10934035 3.767019 97.06331 40-44
TACAC 6994650 3.7646587 26.98793 110-119
GACGG 12903170 3.7510269 108.94012 40-44
AGATA 6188970 3.7268467 20.15091 150-159
ATGGT 10171330 3.7156308 26.29422 190-199
CTGAG 10749650 3.7034943 105.24305 35-39
TGTCC 11270655 3.6799366 20.738432 120-129
CAGGG 12566090 3.6530356 34.794205 50-59
GAGGA 9900075 3.5990016 125.82555 35-39
CCACT 8350125 3.5938733 28.298933 240-247
TGCAC 9150870 3.5237572 112.46952 25-29
GCCCC 12106475 3.5158803 73.32868 60-69
TGGTG 13279390 3.4706986 18.189093 190-199
CTTGG 11834880 3.4572346 22.905493 20-24
AGGCT 10027790 3.4547973 28.804525 30-34
CCGTG 12396175 3.4151933 23.437572 120-129
GGGTT 12925165 3.3781185 41.146305 50-59
CCTGA 8735000 3.3636167 82.35826 30-34
GGATG 10852470 3.3451822 68.18262 20-24
CAGAT 6905905 3.3254771 18.436666 150-159
CTCTC 9109595 3.3244245 13.854211 130-139
TCTTG 9599435 3.3233364 18.383316 170-179
CCAGG 10157765 3.3004878 39.495102 50-59
TCAGG 9558080 3.2929718 21.181606 140-149
GCACT 8421320 3.242827 86.9842 25-29
CTTCA 7059860 3.2218351 17.500557 200-209
TCGCA 8310065 3.1999853 23.811594 100-109
TCTCA 6962820 3.17755 22.049932 130-139
CCTGG 11443960 3.1528547 28.788406 50-59
TGTCT 9097650 3.1496177 19.994993 180-189
CGCAC 8557835 3.1079218 24.334667 100-109
ACAGA 5441035 3.0900629 14.366495 140-149
CGGAT 8779945 3.0248868 77.42576 20-24
GGTTG 11402765 2.9802241 71.49318 15-19
TTCCC 8125365 2.965243 48.50647 50-59
GCCCT 9624835 2.9637876 21.401625 20-24
TGGCC 10720220 2.9534614 43.20594 60-69
GATGC 8452955 2.9122314 93.674545 25-29
GGTTC 9918810 2.8975072 46.46357 50-59
TCTCT 7484300 2.8960497 15.64903 180-189
AGGGT 9345500 2.8806713 49.03549 50-59
GATGG 9340785 2.8792179 22.121162 15-19
TCAAA 4215070 2.8369663 57.59846 70-79
CGGTG 11460855 2.8249998 68.14863 40-44
GACTC 7299375 2.8107953 19.024479 190-199
ACCAC 5522075 2.8030088 19.443916 230-239
GCCGT 10056730 2.7706673 22.203022 120-129
GTTGG 10588760 2.767476 51.34508 15-19
TTCTT 6726355 2.7597644 20.844751 170-179
TCACA 5091585 2.7403913 11.606162 140-149
CGGCC 10538495 2.7382226 12.488335 120-129
TCCAT 5959440 2.7196476 20.07276 70-79
GTTCC 8266560 2.6990817 52.60901 50-59
AGATG 6242095 2.6892893 17.661867 180-189
AGTCA 5556195 2.6755362 30.748695 70-79
CCAGT 6874290 2.6471066 35.060955 60-69
TGGAG 8491900 2.6175563 19.502974 25-29
GGCTG 10587760 2.6097894 14.868667 30-34
TCCTC 6970800 2.5438998 17.27705 130-139
TGTTC 7346390 2.5433292 13.291954 140-149
AGGGA 6883735 2.502463 8.928259 150-159
GCGGA 8557955 2.4878476 84.78691 20-24
ACAGC 5472365 2.4852555 11.614964 120-129
GAGAT 5711435 2.4606645 17.175022 180-189
AATAA 2917485 2.4555445 10.8249235 110-119
ACTCT 5259415 2.4001849 13.63273 200-209
GGCGG 11537940 2.39975 40.71795 20-24
GTTCT 6869180 2.3781185 17.551935 160-169
CTGGA 6867650 2.3660586 15.111324 180-189
CATTT 4850075 2.3468919 18.34939 150-159
TTGGG 8929100 2.3337078 77.341385 15-19
TCATT 4810775 2.327875 16.974566 150-159
CACGG 7153655 2.3243845 9.449294 120-129
CTGGC 8420420 2.3198574 33.97377 60-69
CAGCT 5983890 2.3042374 11.915594 150-159
TTCAT 4751535 2.2992094 16.504906 140-149
TGTAG 6275595 2.292502 7.874971 210-219
TACCA 4246300 2.2854426 16.23432 230-239
GTGAA 5252370 2.262885 18.72074 190-199
CAGAG 5506790 2.237527 9.245785 140-149
GATAC 4605295 2.2176387 14.960183 150-159
TTGTC 6395785 2.2142286 13.538215 170-179
TTCAC 4823225 2.2011251 19.285116 200-209
ATTTG 5055930 2.1888676 16.149647 150-159
ACTAC 4000765 2.1532907 12.827413 230-239
TCAGC 5571325 2.1453693 13.970583 140-149
GAATC 4448265 2.1420224 15.313721 190-199
CTACT 4691165 2.1408582 7.9161024 230-239
CTCGC 6849730 2.1092458 16.16183 100-109
TACAG 4369175 2.1039374 14.143172 160-169
ATGTA 4092025 2.0893397 8.804303 70-79
TGAAT 4087665 2.0871136 14.777468 190-199
GTCCT 6384845 2.0846903 14.615972 130-139
GCTGT 7134165 2.08405 10.38043 140-149
CACTA 3865625 2.0805554 15.3044195 240-247
CTCTG 6361040 2.0769176 14.975746 180-189
TTGGA 5680555 2.0751312 15.738432 170-179
GTCTC 6338260 2.0694802 12.234618 180-189
ATATA 2895045 2.066056 10.945889 110-119
GTTCA 5052435 2.062917 15.441518 140-149
TCTGC 6302805 2.0579038 12.404263 210-219
ACACA 3236565 2.0544536 13.953686 110-119
CAGGC 6320210 2.0535793 15.566916 140-149
TGGGG 9294685 2.0497923 50.82604 15-19
TAGTC 5007710 2.0446558 25.117788 70-79
TCTCG 6258245 2.0433547 15.749657 100-109
AATCG 4231450 2.0376172 13.9261465 190-199
AATAT 2850015 2.0339203 13.62791 110-119
ATCTC 4453200 2.032261 8.123334 100-109
TTGCA 4958820 2.0246937 14.579857 150-159
GAACT 4191125 2.018199 10.721197 160-169
AGCTC 5219350 2.0098329 11.972428 140-149
TTTGC 5776430 1.9998072 12.402329 150-159
GTCAA 4109455 1.9788716 39.23684 70-79
TGGAA 4592105 1.9784222 9.3015175 170-179
ACGGC 6087280 1.9778951 14.324079 120-129
AACCA 3104705 1.9707534 14.867862 70-79
GATAT 3821425 1.9511745 7.955398 180-189
CCAGA 4293505 1.9498802 29.781677 60-69
GAGTC 5612940 1.933783 12.808355 210-219
CCTTC 5294975 1.9323303 14.333116 200-209
ACACG 4238680 1.9249818 13.255087 120-129
CAGCC 5288680 1.9206733 10.528988 120-129
TCGGC 6967815 1.9196593 7.8846645 130-139
ATATG 3713265 1.8959494 8.194134 180-189
TACTA 3306360 1.8868926 10.487947 230-239
GACGT 5448405 1.877097 9.4904585 170-179
CATAG 3876970 1.8669206 8.448211 210-219
CCTTG 5676210 1.8533168 26.05438 20-24
GGGGC 8807300 1.8318105 46.236385 15-19
CTGTT 5282545 1.8288234 13.049736 140-149
AGTAT 3510465 1.792402 12.378869 220-229
GGGCG 8595220 1.7877004 56.94484 20-24
GTTGT 5754390 1.7823857 6.933809 210-219
GCAGA 4376715 1.7783532 14.821201 150-159
AGAAC 3110365 1.7664329 11.421268 160-169
CGTAG 5098445 1.7565283 9.920039 210-219
ATTGT 4042900 1.7502958 8.221672 50-59
TGCAG 5077360 1.749264 13.7492 150-159
AGTCT 4194875 1.7127738 13.547265 210-219
GTCCC 5520325 1.6998806 22.227032 50-59
GGTCA 4930190 1.6985605 7.5206275 130-139
CTCCA 3907135 1.6816212 7.682981 150-159
CAAAA 2118295 1.6814665 22.34569 70-79
GTCAC 4358295 1.6782638 8.459507 140-149
ATAGT 3285375 1.6774739 7.5860367 220-229
TCAGA 3456785 1.664584 10.207429 130-139
TTGGT 5359130 1.6599565 27.526188 25-29
CACCA 3255785 1.6526386 13.584282 230-239
TGACT 4038770 1.6490359 11.578372 190-199
AACTG 3415845 1.6448698 10.62589 160-169
GAGAA 3219890 1.6360651 10.553681 160-169
TCCCA 3795195 1.6334425 11.048231 240-247
TGGTT 5237050 1.6221429 17.499388 10-14
AATAG 2682695 1.6154535 9.633347 240-247
TCTGG 5506885 1.6086849 12.198245 180-189
TTCAG 3908630 1.5958996 9.246804 150-159
CAGAC 3477445 1.5792694 17.902319 60-69
CTGTG 5397680 1.5767835 5.3665557 210-219
GAACC 3466115 1.574124 12.239454 70-79
ATAGA 2605820 1.5691614 10.264502 240-247
GTCTG 5364785 1.5671743 9.261951 210-219
ACCAT 2906300 1.5642279 12.560419 45-49
GCTCC 5074360 1.562554 6.171276 150-159
GGCCG 6696280 1.5566735 12.903828 120-129
ACTGA 3225985 1.5534445 8.518142 180-189
GCTGA 4486550 1.5457168 24.415632 30-34
TGCTC 4731405 1.5448322 5.326117 150-159
TGTGC 5284695 1.5437783 8.134922 220-229
CTGGT 5274130 1.5406919 6.789219 160-169
TTGTA 3554965 1.5390536 8.889789 210-219
CAAAG 2697915 1.532195 28.049654 70-79
GCTCA 3966080 1.527232 10.724664 140-149
GGACG 5228775 1.5200354 7.118619 170-179
TAGTT 3498650 1.5146731 9.656869 220-229
CCTCG 4911200 1.512312 9.841785 130-139
GTGCT 5146340 1.5033617 7.840093 220-229
GCTCT 4536300 1.4811293 9.983154 130-139
CATGT 3624835 1.4800256 8.782704 70-79
CCCAC 3642680 1.4786092 10.637375 240-247
CCACC 3626400 1.472001 9.717813 230-239
CGACT 3694885 1.4228022 8.411462 190-199
ACCGT 3674380 1.4149063 21.01049 45-49
GTGGA 4580935 1.4120346 24.574726 25-29
AATTG 2749275 1.4037474 9.29341 170-179
TCGAC 3629500 1.397624 9.119182 190-199
TATAC 2445370 1.3955379 6.843784 110-119
CCTCA 3219985 1.3858737 7.476786 130-139
CCATC 3218180 1.3850968 11.326689 240-247
TGGAC 4009650 1.3814138 10.215602 170-179
CTCTT 3552140 1.3745004 8.044702 200-209
TGATA 2689100 1.3730228 7.458535 180-189
CACTG 3553895 1.3685105 7.1821027 230-239
GTCCA 3518095 1.3547249 18.387804 70-79
CCATG 3499640 1.3476185 8.887108 70-79
GCGTA 3906730 1.3459557 10.545539 210-219
AAAAA 1350760 1.3408177 8.120425 8
GGGAG 5135380 1.3356731 6.371765 150-159
ACGGA 3285140 1.3348229 9.634064 200-209
GTAGG 4280725 1.3194972 5.126419 160-169
GAATT 2570025 1.3122244 10.352091 170-179
AGCCG 4029980 1.3094317 9.390483 120-129
ACTTC 2867390 1.3085611 6.96199 220-229
CTCGA 3393970 1.3069277 8.109531 190-199
CTGCT 3987855 1.3020586 5.4935446 90-99
AGATC 2703705 1.3019451 6.8263726 130-139
GAGGG 5005310 1.3018428 7.975795 200-209
GGAGG 4976935 1.2944628 18.572739 30-34
TATGG 3541910 1.2938751 6.0479035 190-199
CTGAT 3139865 1.2820115 7.061211 180-189
ATCGG 3698530 1.2742262 8.231381 190-199
GTGTT 4113345 1.2740824 7.6801004 160-169
TTGGC 4350905 1.2709972 8.827366 60-69
TCCAC 2948680 1.2691045 9.590319 240-247
CTTGA 3088000 1.2608352 8.1970005 200-209
TGGTC 4301740 1.256635 13.545042 50-59
ACGTG 3646665 1.2563576 9.097902 170-179
GGAGT 4067235 1.2536908 7.1956983 210-219
AAGTA 2079255 1.2520766 15.705792 70-79
GGCTC 4533870 1.2490983 8.361292 130-139
GTGGT 4772080 1.2472299 11.958829 50-59
ATAAA 1477170 1.2432821 5.704736 7
ATAAC 1827845 1.2302369 7.4643693 80-89
ACTAT 2152880 1.2286179 6.481577 230-239
GATCT 2984375 1.2185248 5.3945713 130-139
GGAAT 2807900 1.209731 8.651766 170-179
AAAGT 2003745 1.2066064 20.561157 70-79
TTCCA 2632000 1.2011386 7.782778 230-239
TGCCC 3900525 1.2010936 7.541187 60-69
AGTTC 2931390 1.1968911 5.6682863 160-169
ACTCG 3107600 1.1966543 8.329056 190-199
CCATA 2189495 1.1784295 11.359909 70-79
ACTGG 3418945 1.1779029 7.0035515 160-169
ATACC 2181025 1.1738706 8.240835 70-79
TTGAG 3203165 1.17013 7.8914084 200-209
GCATA 2428570 1.1694564 8.059075 210-219
TATCA 2035840 1.161825 7.3094363 70-79
CAATA 1710990 1.1515874 6.7775435 100-109
AGCTT 2790960 1.1395532 5.9832435 150-159
ATGGG 3696675 1.1394688 34.900726 15-19
TCACG 2949300 1.135697 9.713949 200-209
ACTGT 2776065 1.1334716 5.5118165 230-239
AAAAG 1581495 1.1231645 15.075136 70-79
TCTGT 3198840 1.1074424 6.592523 210-219
AGGTT 3029555 1.1067095 22.882345 10-14
AGAGC 2716265 1.1036767 6.7227745 140-149
GAAGA 2158295 1.0966558 19.344723 35-39
CGGCT 3976885 1.0956469 7.8821692 130-139
CTCGG 3975545 1.0952777 8.379606 130-139
AGAGA 2143995 1.0893899 14.253368 35-39
GTCTA 2650375 1.0821521 7.3222823 180-189
ATATC 1891535 1.079472 7.793052 70-79
TCTAC 2356935 1.0756099 7.1291656 180-189
CTGAA 2182740 1.051079 15.716683 35-39
ATCCA 1948715 1.0488368 15.588998 240-247
AACTT 1824810 1.041393 5.973762 210-219
GTCCG 3767530 1.0379688 5.0137734 130-139
GAGCT 3008790 1.0365952 5.9993415 140-149
TATGT 2392620 1.0358388 7.697957 220-229
TACTG 2536000 1.0354527 6.162946 180-189
GCTTC 3153595 1.0296677 5.2997327 150-159
CTGCC 3343530 1.0295773 5.8688455 200-209
GCGGT 4136180 1.019532 5.674256 130-139
CATGG 2957390 1.0188869 5.327938 190-199
CTGCG 3688115 1.0160896 7.017371 210-219
ATACG 2090315 1.0065726 5.208832 120-129
AAAAT 1185340 0.9976589 5.1609755 8
TCCCC 2868030 0.98710567 5.3157496 80-89
TAGAT 1930380 0.9856293 7.873135 240-247
TATAA 1378950 0.9840911 6.7074094 240-247
ACAAT 1461300 0.9835326 7.8147583 100-109
CGAAC 2155325 0.9788333 12.815698 70-79
CTACC 2266340 0.97542715 8.2916355 230-239
TGCGT 3327100 0.9719207 8.339651 210-219
CGTGG 3928910 0.9684417 8.197248 50-59
CCATT 2110635 0.9632086 6.1915383 50-59
CTTCT 2479225 0.9593361 5.3567395 210-219
GGGGT 4346235 0.9584917 7.3102007 10-14
TAGGC 2764845 0.95255077 5.7667303 160-169
GAGTT 2595170 0.9480268 5.3839135 160-169
CAGCG 2912650 0.9463859 6.2423477 160-169
ACGTC 2448215 0.94274265 12.555657 70-79
AAGAG 1851590 0.94081527 14.357994 35-39
TGAAG 2181135 0.9397011 10.408772 35-39
CGGAG 3229720 0.93889844 6.704509 210-219
GAGGC 3227200 0.93816584 15.822993 30-34
ACCCA 1806770 0.9171176 12.963222 240-247
GGTGG 4144445 0.91399014 24.29798 25-29
CCCAA 1797600 0.912463 9.555735 240-247
TCGAA 1870390 0.90066963 16.55377 70-79
TGTAA 1751650 0.8943718 6.659258 230-239
ATCAA 1328745 0.89431614 13.988522 70-79
TGCAT 2175005 0.8880579 6.1152077 210-219
GGGCC 3807575 0.8851409 18.320414 15-19
ACTAA 1306155 0.8791118 12.622095 240-247
CATCA 1629075 0.8768003 5.9038596 230-239
CGTCC 2759785 0.8498241 10.21864 70-79
TTCTG 2449520 0.8480269 5.3956923 210-219
CGGTC 3066925 0.8449495 5.3906293 130-139
CCAAT 1567035 0.8434092 10.545081 240-247
TGGGC 3392415 0.8362004 19.910307 15-19
AGCGT 2412960 0.83131856 6.445044 160-169
GACCG 2550700 0.8287801 19.27961 45-49
CCAGC 2263995 0.8222079 5.557096 80-89
TGAAC 1703565 0.8203366 6.482699 190-199
GGTCC 2955750 0.81432027 13.658974 50-59
TAACC 1465730 0.78888476 5.950126 80-89
CTTCC 2145550 0.78298974 5.4240723 230-239
GTATG 2138845 0.7813293 5.304302 220-229
AACCG 1716085 0.779354 5.77577 190-199
TGAGA 1758700 0.7577029 5.928326 240-247
AAAGG 1467755 0.74578404 8.780549 10-14
AGCCA 1621695 0.73648703 5.7699137 80-89
CATAC 1367750 0.73615 9.765042 70-79
AAAGA 1036435 0.7360675 5.986032 8
GTCGA 2100960 0.72382766 11.572225 70-79
CCTTT 1865890 0.72200614 5.1805916 50-59
CTAAT 1245205 0.7106207 9.85249 240-247
GTAGC 2057130 0.7087272 5.492838 80-89
AATCC 1313780 0.7071023 9.288483 240-247
AAAGC 1238010 0.7030884 10.255122 70-79
ATGAT 1361460 0.6951455 6.928847 240-247
AAGGT 1606980 0.69233716 9.8569765 10-14
CATTG 1673930 0.6834682 5.668837 50-59
CGATG 1946535 0.6706248 15.205033 15-19
AGGAT 1554260 0.66962373 6.554716 10-14
GGAAG 1780290 0.64719373 6.6416655 30-34
TAGGT 1759380 0.6427091 5.3409147 10-14
AGAAG 1252135 0.6362249 5.6640835 9
CCGTA 1650175 0.63543856 5.408596 80-89
GAGGT 2031255 0.62611717 6.659914 10-14
ATAGG 1437805 0.61945134 5.2606235 10-14
TATGA 1209950 0.6177862 5.8549466 240-247
CAATC 1137455 0.61220074 9.037164 240-247
AGACC 1338775 0.608 5.0910764 240-247
AATGG 1331335 0.57358074 6.6156235 10-14
TAAAA 670615 0.56443304 5.688123 8
CATCC 1308165 0.563031 7.4639373 240-247
AAATG 932070 0.56126976 5.3899794 9
GGGTC 2226310 0.5487658 5.5207367 70-79
AAGCA 938575 0.5330338 6.9067535 70-79
AAGAA 750385 0.53291714 5.476218 8
GAAGG 1444595 0.52515763 5.467004 10-14
GAAAA 714400 0.50736094 5.9492497 8
AAGGG 1391750 0.5059467 6.191408 10-14
ACGAT 1027680 0.49487016 6.704725 10-14
GGTCG 1953165 0.48143795 5.587663 70-79
ATAAG 798430 0.48079506 5.9954734 9
TGATC 1174050 0.47936642 5.3695617 240-247
TTGCC 1465555 0.47851253 5.3204618 60-69
GATCC 1198630 0.46156055 5.3914876 240-247
CGGTT 1516380 0.4429687 7.852109 10-14
TAAGG 1022640 0.4405853 5.962974 10-14
GAAGC 1080455 0.43901205 5.4247103 30-34
TACCG 1130050 0.43515226 5.154831 70-79
AAGCT 878950 0.42325056 9.113558 30-34
TAAAG 665385 0.4006786 5.6495805 9