FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_7_SL248353.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_7_SL248353.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5708550
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 6423355 8.872679 61.430412 110-119
ATACA 5916085 7.6838036 37.452995 110-119
CACAG 8667080 7.563027 41.61615 110-119
TCCCT 10027790 7.077283 102.64351 30-34
CAGTA 7178240 6.6618037 38.899437 110-119
AGTAA 5635170 6.5417113 47.549873 110-119
GGAGA 8960045 6.2462325 94.42979 35-39
GTAAT 6096490 6.017343 39.407963 110-119
GGTGA 9994520 5.923937 101.475204 45-49
CCCTG 9811255 5.8193893 66.88033 30-34
AATAC 4301805 5.587178 39.41542 110-119
AGACG 7080750 5.5226135 101.7245 40-44
GTAGT 7644805 5.3916707 32.192856 80-89
ACAGT 5735640 5.322991 35.41804 110-119
GTGAC 7734755 5.1292334 84.83939 45-49
CCCCA 6243825 4.873271 101.44601 60-69
GAGAC 6248050 4.8731513 141.08722 40-44
TAGTA 4918730 4.854873 41.847965 80-89
CTCAG 6409470 4.755383 34.914604 140-149
ACTCC 5593180 4.6427956 115.55919 30-34
ACCAG 5178475 4.518817 89.370544 45-49
GGCCC 8975585 4.474115 57.646477 60-69
GCACA 5106420 4.4559402 34.036407 110-119
TGACC 5927145 4.397531 145.94095 45-49
GTGTC 7797385 4.3963814 23.524479 120-129
AGTAG 5289780 4.3878727 33.562443 80-89
CGTGT 7716510 4.3507824 22.985895 120-129
CACTC 5121310 4.2511053 95.01283 25-29
CTCCC 6202280 4.11587 66.14974 30-34
CCCAG 5828910 4.066303 86.66912 60-69
ATGCA 4276770 3.9690793 92.84893 25-29
GACCA 4513890 3.9388897 103.30059 45-49
CCCTT 5499730 3.8815277 41.728107 20-24
TGAGG 6455840 3.8264954 68.598015 35-39
TACAC 3652295 3.7922518 27.162445 110-119
AGGAG 5405435 3.7682405 94.58412 35-39
ACGGT 5633940 3.7360969 96.64406 40-44
GACGG 6683230 3.7246363 107.798584 40-44
ATGGT 5247025 3.7005823 25.871162 190-199
TGTCC 5821600 3.6723683 20.392134 120-129
AGATA 3158125 3.666179 19.277308 150-159
CTGAG 5514675 3.6570072 103.6872 35-39
CCACT 4329845 3.5941246 28.325994 240-247
CAGGG 6430960 3.5840435 33.362526 50-59
GAGGA 5105985 3.5594878 124.2602 35-39
TGGTG 6956370 3.5056746 18.804802 25-29
GCCCC 6264355 3.4936404 72.440765 60-69
CTTGG 6183530 3.4864457 23.808043 20-24
TGCAC 4692585 3.481573 111.19217 25-29
AGGCT 5212430 3.4565759 29.321554 30-34
CCGTG 6492095 3.4417577 23.017653 120-129
CAGAT 3680770 3.415958 17.70761 150-159
GGATG 5638145 3.3418324 67.424324 20-24
CCTGA 4485100 3.3276336 81.47079 30-34
GGGTT 6584605 3.3183234 39.53416 50-59
TCTTG 4936595 3.3119392 17.97556 170-179
CTCTC 4678190 3.301712 13.557094 100-109
TCAGG 4930085 3.2693417 20.821358 140-149
CTTCA 3646745 3.2194169 16.586208 200-209
GCACT 4314140 3.200793 86.17463 25-29
CCAGG 5131905 3.1998787 37.948208 50-59
TCGCA 4298515 3.1892009 23.60764 100-109
TCTCA 3561380 3.1440551 21.593594 130-139
TGTCT 4646710 3.1174567 19.618284 180-189
CCTGG 5861570 3.1074874 27.784637 50-59
CGCAC 4434425 3.0934966 24.092972 100-109
ACAGA 2827285 3.0860498 14.396509 140-149
CGGAT 4505960 2.988087 76.63408 20-24
GCCCT 5007260 2.9699764 22.118855 20-24
GGTTG 5886260 2.9663908 70.49333 15-19
TTCCC 4104855 2.8970711 46.959934 50-59
TGGCC 5442080 2.8850965 42.13389 60-69
GATGG 4848690 2.873908 22.755318 15-19
GACTC 3872435 2.8730786 19.74768 190-199
GATGC 4331895 2.8726573 92.180954 25-29
GGTTC 5060285 2.8531287 44.71404 50-59
TCTCT 3768895 2.828957 14.909424 180-189
AGGGT 4749035 2.8148406 47.22478 50-59
CGGTG 5896770 2.7941656 67.766205 40-44
ACCAC 2848405 2.7808788 19.003178 230-239
GCCGT 5230615 2.7729893 21.73732 120-129
TCAAA 2128295 2.7642267 55.739567 70-79
TTCTT 3443355 2.7488127 20.603954 170-179
GTTGG 5430640 2.736781 50.793392 15-19
TCCAT 3096395 2.7335572 20.972761 70-79
CGGCC 5429690 2.7065709 12.504268 120-129
TCACA 2587685 2.6868458 11.68179 140-149
AGTCA 2842235 2.637751 29.135408 70-79
AGATG 3164005 2.6245422 16.89433 180-189
CCAGT 3518930 2.6108024 33.94629 60-69
TGGAG 4388300 2.6010263 20.105022 25-29
GGCTG 5483980 2.5985663 15.150025 30-34
GTTCC 4118655 2.5981205 50.660477 50-59
AGGGA 3653495 2.546927 9.124226 150-159
TCCTC 3581200 2.5274928 16.78733 130-139
TGTTC 3689425 2.4752185 12.65017 140-149
ACTCT 2789265 2.462417 14.014291 200-209
GCGGA 4394835 2.449289 83.378716 20-24
ACAGC 2764665 2.4124892 10.939246 120-129
GAGAT 2899095 2.4047995 16.404049 180-189
AATAA 1467830 2.3846688 10.41081 110-119
GGCGG 5948590 2.3688827 39.95838 20-24
TGTAG 3355470 2.3665204 8.1976385 210-219
GTTCT 3513975 2.3575099 17.36357 160-169
CAGCT 3153195 2.3394523 12.190729 150-159
CTGGA 3508400 2.3265643 14.35994 180-189
CACGG 3698160 2.3059008 9.644778 120-129
TTGGG 4572140 2.3041382 76.46484 15-19
TACCA 2193295 2.2773426 16.15251 230-239
CATTT 2423870 2.27579 17.67243 150-159
TCATT 2407080 2.2600257 16.447685 150-159
TTCAT 2391755 2.245637 15.779564 140-149
CTGGC 4229905 2.2424672 32.583748 60-69
CAGAG 2872270 2.2402198 9.303347 140-149
TCAGC 2976820 2.2085943 14.325698 140-149
GTGAA 2631855 2.1831238 17.738342 190-199
TTGTC 3244325 2.176603 12.999564 170-179
GATAC 2340705 2.1723037 14.387826 150-159
ATGTA 2188515 2.1601028 9.3709345 70-79
CTACT 2429260 2.1445978 7.6779056 230-239
TTCAC 2424115 2.1400557 18.229473 200-209
ATTTG 2540030 2.1315928 15.499326 150-159
CCAGA 2438260 2.1276631 31.718094 60-69
CTCGC 3571860 2.1185918 16.15974 100-109
ACTAC 2031465 2.109311 12.471966 230-239
GAACT 2267055 2.1039524 10.921637 160-169
TCTCG 3290020 2.0754027 15.884519 100-109
ATCTC 2347640 2.072542 8.061979 100-109
GAATC 2223735 2.063749 14.443947 190-199
GCTGT 3655235 2.060923 9.914842 140-149
TCTGC 3261585 2.0574656 11.990576 210-219
CTCTG 3258950 2.0558033 14.37098 180-189
CACTA 1978885 2.0547163 15.104741 240-247
AGCTC 2765905 2.05211 12.3462925 140-149
GTCCT 3250265 2.050325 14.193875 130-139
TTGGA 2892740 2.04017 15.367405 170-179
TACAG 2196355 2.038339 13.512295 160-169
ACACA 1661345 2.0288532 13.614142 110-119
TGGGG 4782975 2.0257163 50.166164 15-19
TGAAT 2051075 2.0244472 13.9913845 190-199
GTCTC 3202590 2.0202503 11.697053 180-189
GTTCA 2555720 2.0166366 14.664432 140-149
GATAT 2037560 2.0111074 8.32843 180-189
CAGGC 3225080 2.010923 15.114268 140-149
ACACG 2293290 2.0011601 13.584331 120-129
TAGTC 2533580 1.9991668 24.07664 70-79
ACGGC 3197955 1.9940096 14.3445215 120-129
AATCG 2147110 1.9926369 13.347699 190-199
TTTGC 2966645 1.9903088 11.851861 150-159
TTGCA 2501285 1.9736837 13.955197 150-159
ATATA 1423515 1.9663231 10.3102255 110-119
TGGAA 2367155 1.9635551 8.783423 170-179
AATAT 1405625 1.9416114 12.945697 110-119
GACGT 2923700 1.9388255 10.032153 60-69
AACCA 1584005 1.9344047 14.559481 70-79
CCTTG 3043245 1.9197328 27.132816 20-24
GTCAA 2051425 1.9038359 37.296246 70-79
ATATG 1925975 1.9009713 8.584591 180-189
CAGCC 2721915 1.8988335 9.980478 120-129
CCTTC 2670090 1.8844613 13.62843 200-209
GAGTC 2808480 1.8624183 12.132716 210-219
CATAG 2005090 1.8608342 8.480029 210-219
TCGGC 3500700 1.8558819 7.4813366 130-139
TACTA 1673845 1.8484068 10.236884 240-247
GGGGC 4515970 1.7983761 45.51809 15-19
CTGTT 2677120 1.7960677 12.370708 140-149
AGAAC 1638675 1.7886533 11.473567 160-169
GTTGT 2964360 1.7775769 7.0983844 210-219
AGTAT 1779925 1.7568172 11.9497 220-229
GGGCG 4402655 1.7532513 55.68157 20-24
CGTAG 2636865 1.7486134 9.610037 210-219
GTCCC 2947235 1.7481055 23.370092 50-59
CTCCA 2103975 1.746471 7.928776 150-159
CACCA 1787395 1.7450217 13.228772 230-239
ATTGT 2057185 1.7263894 8.567916 50-59
GTCAC 2325045 1.7250224 8.651429 130-139
GGTCA 2599715 1.7239777 7.653947 130-139
GCAGA 2208490 1.7225065 14.020861 150-159
TTGGT 2835580 1.700354 28.558018 25-29
TCAGA 1827995 1.6964805 10.481189 130-139
TGACT 2149965 1.6964684 11.931341 190-199
TGCAG 2556670 1.6954328 13.00331 150-159
CAAAA 1095240 1.6730565 22.365171 70-79
ATAGT 1686510 1.6646152 7.5016294 220-229
AGTCT 2098010 1.6554723 12.841826 210-219
AACTG 1783590 1.65527 10.706807 160-169
TCCCA 1992700 1.6541036 11.602436 240-247
GAGAA 1695155 1.6538087 10.647177 160-169
CAGAC 1892265 1.6512195 19.16387 60-69
TGGTT 2735595 1.6403979 17.56543 10-14
TTCAG 2072860 1.6356273 9.38104 150-159
CTGTG 2878530 1.622995 5.556752 210-219
AATAG 1378845 1.6006626 9.558787 240-247
GCTCC 2676465 1.5875027 6.311581 150-159
AAAAA 829415 1.5848336 7.874942 8
TGCTC 2502420 1.5785708 5.4812713 150-159
TGTGC 2798760 1.5780184 8.216504 220-229
ACTGA 1699640 1.5773599 8.744549 180-189
ATAGA 1355095 1.573092 10.444748 240-247
GCTCA 2118905 1.572081 11.132775 140-149
ACCAT 1512330 1.5702828 13.169416 45-49
CTGGT 2784970 1.5702435 6.9481173 160-169
GTCTG 2768200 1.5607879 8.815348 210-219
GGCCG 3502780 1.5606294 12.815966 120-129
GCTGA 2342415 1.5533516 24.809914 30-34
TCTGG 2754990 1.5533397 11.591964 180-189
TTGTA 1847305 1.5502582 9.198222 210-219
GAACC 1768765 1.5434514 11.9861145 70-79
GGACG 2765715 1.5413626 7.169416 170-179
TAGTT 1828525 1.534498 9.918867 220-229
CATGT 1943170 1.533293 9.34424 70-79
GTGCT 2701095 1.5229522 7.9346285 220-229
CAAAG 1364770 1.4896793 26.62296 70-79
CCCAC 1898210 1.4815423 10.631795 240-247
AGATC 1593985 1.4793062 7.167255 130-139
ACCGT 1992080 1.4779854 22.58304 45-49
CCTCG 2487680 1.4755278 9.320624 130-139
GTGGA 2475445 1.467242 25.423086 25-29
CCACC 1873600 1.4623343 9.400667 230-239
GCTCT 2292480 1.4461371 9.533959 130-139
CGACT 1931510 1.4330467 8.742728 190-199
TCGAC 1897435 1.4077654 9.396522 190-199
CCTCA 1693150 1.4054527 7.4833207 130-139
TGATA 1423795 1.4053108 7.705393 180-189
CCATG 1879780 1.3946666 9.446866 70-79
CTCTT 1851400 1.3896729 8.252725 200-209
GTCCA 1872590 1.3893323 19.471212 70-79
TGGAC 2093370 1.3881996 10.2679205 170-179
CACTG 1846860 1.3702422 7.390491 230-239
GTAGG 2299525 1.3629708 5.2701592 160-169
TATAC 1232235 1.3607424 6.587356 110-119
AATTG 1375535 1.3576772 8.765817 170-179
GGGAG 2697705 1.3437994 6.5319695 150-159
CTGCT 2117400 1.3356935 5.508455 90-99
ACTTC 1504120 1.3278661 6.8991594 220-229
CTCGA 1783525 1.3232521 8.394917 190-199
GAGGG 2655200 1.3226265 8.072861 200-209
CCATC 1591685 1.3212285 11.40952 240-247
TATGG 1871050 1.3195999 6.2761426 180-189
GGAGG 2647745 1.318913 18.962214 30-34
GCGTA 1987585 1.3180492 10.184466 210-219
TGGTC 2328260 1.3127375 14.484768 50-59
CTGAT 1659340 1.3093319 7.320936 180-189
GTGGT 2594875 1.3076918 12.835738 50-59
CTTGA 1641115 1.2949512 8.434657 200-209
TTGGC 2284245 1.2879206 9.266099 60-69
ACGGA 1640030 1.2791375 9.0763645 200-209
AGCCG 2050210 1.2783602 8.886194 120-129
TCCAC 1532585 1.2721707 9.935625 240-247
ACGTG 1916095 1.2706412 9.288089 170-179
ATCGG 1908225 1.2654223 7.8924894 190-199
GTGTT 2109515 1.2649695 7.317742 160-169
GAATT 1279315 1.2627064 9.749941 170-179
TGCCC 2102570 1.247106 8.102492 60-69
GATCT 1571265 1.2398347 5.6868415 130-139
AAGTA 1061380 1.2321264 15.212307 70-79
ACTAT 1114125 1.2303146 6.599637 230-239
ACTCG 1644990 1.2204686 8.558692 190-199
GGCTC 2300285 1.2194867 7.9356036 130-139
ATAAA 743885 1.208532 5.545841 7
GGAGT 2030745 1.20366 6.7679567 210-219
CCATA 1158060 1.2024372 12.041087 70-79
ATAAC 921840 1.1972846 7.0316596 80-89
GGAAT 1437780 1.1926385 8.160011 170-179
ACTGG 1795275 1.1905204 7.041267 160-169
TTGAG 1680315 1.1850798 8.156528 200-209
ATACC 1141200 1.1849312 8.751955 70-79
ATGGG 1984205 1.1760749 35.99812 15-19
GCATA 1259825 1.1691874 8.037251 210-219
AGCTT 1468225 1.1585293 6.1698146 150-159
AGAGC 1482990 1.1566544 6.9293323 140-149
AAAGT 991940 1.1515154 19.399994 70-79
GAAGA 1178940 1.1501846 19.827852 35-39
TATCA 1040960 1.1495196 7.4791408 70-79
AGTTC 1448165 1.1427004 5.5308943 160-169
ACTGT 1446520 1.1414025 5.6809773 230-239
TTCCA 1279510 1.1295762 7.537396 230-239
AAAAG 826415 1.1283453 15.31297 70-79
TCTGT 1680790 1.1276344 6.710879 210-219
CAATA 865915 1.1246492 6.6663923 100-109
AGAGA 1145550 1.117609 14.679732 35-39
ATATC 1007195 1.1122334 7.952228 70-79
AGGTT 1568170 1.1059872 22.752756 10-14
CTGAA 1190625 1.1049659 15.917418 35-39
TCTAC 1250660 1.1041068 7.2924595 180-189
GTCTA 1394800 1.1005919 7.442036 180-189
ATCCA 1053845 1.0942286 16.359873 240-247
AACTT 986125 1.0889658 6.4501276 200-209
TCACG 1462715 1.0852332 9.107081 200-209
CTCGG 2004875 1.0628763 7.903091 130-139
TACTG 1343305 1.0599588 6.3924046 180-189
CGGCT 1996555 1.0584655 7.4467463 130-139
GAGCT 1587920 1.0530148 6.213661 140-149
GTCCG 1984255 1.0519447 5.080791 130-139
GCTTC 1657745 1.0457349 5.4448533 150-159
CTGCC 1759430 1.0435779 5.9558873 200-209
CATGG 1552830 1.0297452 5.447128 190-199
ACGTC 1382825 1.0259604 13.3800125 70-79
GCGGT 2153885 1.0206116 5.7373877 130-139
CGTGG 2144975 1.0163897 8.753781 50-59
TATGT 1206335 1.0123562 7.4389267 220-229
CTTCT 1346240 1.0104965 5.6931186 210-219
TCCCC 1520005 1.0086844 5.345009 80-89
TAGAT 1021585 1.0083224 8.005492 240-247
AAGAG 1024170 0.9991896 14.917039 35-39
CTGCG 1884425 0.9990203 6.6311293 210-219
CGAAC 1120090 0.97740775 12.582634 70-79
AAAAT 599320 0.9736684 5.0197306 8
TATAA 701320 0.9687441 6.519292 240-247
ACAAT 744985 0.96758544 7.714168 100-109
CTACC 1160875 0.963621 8.189843 230-239
TAGGC 1450740 0.96204525 5.9039364 160-169
CCATT 1089220 0.96158445 6.4383874 50-59
GAGGC 1710965 0.9535392 16.069284 30-34
TGCGT 1690235 0.95300144 8.051951 210-219
GGTGG 2247580 0.95190954 25.057024 25-29
AGCTG 1431395 0.9492168 5.0520754 30-34
GGGGT 2233945 0.94613475 7.1746016 10-14
TGAAG 1140505 0.946049 10.570756 35-39
GAGTT 1330160 0.93812525 5.2477646 160-169
CCCAA 956785 0.9341028 10.081493 240-247
ACCCA 946380 0.9239444 12.909062 240-247
CAGCG 1464635 0.9132387 5.8841047 160-169
AGTTT 1075655 0.9026895 5.000103 220-229
ATCAA 694880 0.9025092 14.208677 70-79
TCGAA 971160 0.90129024 16.541044 70-79
GGGCC 2021005 0.9004391 18.919537 15-19
CGGAG 1615100 0.9001127 6.3339744 210-219
TGCAT 1134970 0.89556843 6.1671405 210-219
CGTCC 1503475 0.8917623 10.871134 70-79
TGTAA 901145 0.88944596 6.649729 230-239
GACCG 1425705 0.88896483 20.558168 45-49
TTCTG 1317615 0.88398194 5.5995274 210-219
ACTAA 663290 0.8614801 12.2317095 240-247
TGGGC 1816090 0.8605485 20.602974 15-19
CATCA 826890 0.8585766 5.613921 230-239
CGGTC 1605220 0.85100085 5.49943 130-139
CCAAT 818790 0.8501662 10.934305 240-247
GGTCC 1601990 0.84928846 14.498892 50-59
TGAAC 894425 0.8300759 6.201059 190-199
CCAGC 1183435 0.8255754 5.521944 80-89
AGCGT 1208385 0.8013297 6.0662017 160-169
GTATG 1133600 0.7994969 5.2185893 220-229
CTTCC 1094915 0.77275485 5.277323 230-239
TAACC 739720 0.76806635 5.619682 80-89
CATAC 735780 0.7639752 10.445146 70-79
AACCG 861960 0.75215954 5.425499 190-199
TGAGA 904795 0.7505275 5.7217026 240-247
AAAGA 548285 0.7486007 5.850015 8
AAAGG 759845 0.7413117 8.819505 10-14
CCTTT 979405 0.7351478 5.5987177 50-59
AGCCA 836085 0.7295806 5.6362853 80-89
AATCC 700220 0.7270526 9.754835 240-247
GTCGA 1084285 0.71903396 11.38565 70-79
ATGAT 728270 0.7188153 7.0347877 240-247
AAAGC 653095 0.7128689 10.398664 70-79
GTAGC 1073150 0.7116498 5.422051 80-89
AAGGT 840470 0.6971699 9.65678 10-14
CATTG 882115 0.6960486 5.8807626 50-59
TGTTA 826800 0.69385046 5.201892 230-239
CTAAT 626800 0.69216764 9.382401 240-247
CGATG 1042150 0.6910925 15.771811 15-19
GGAAG 988200 0.6888946 6.8974667 30-34
AGGAT 813220 0.67456603 6.801155 10-14
TAGGT 956230 0.6744027 5.3414464 10-14
ATCCC 795215 0.66009337 5.0489044 240-247
CCGTA 886410 0.6576548 5.532192 80-89
GAGGT 1094455 0.64870363 6.6412153 10-14
CCCAT 777795 0.64563334 5.119728 240-247
AGAAG 652680 0.6367606 5.344789 9
TATGA 644400 0.63603413 5.862101 240-247
AGACC 727040 0.63442624 5.031827 240-247
CAATC 602900 0.62600327 9.443804 240-247
ATAGG 738555 0.61263144 5.217287 10-14
CATCC 714685 0.5932469 7.9628882 240-247
AATGG 682665 0.56627065 6.5288777 10-14
TAAAA 343805 0.55855316 6.059574 8
GGGTC 1167145 0.55304796 5.385619 70-79
AAATG 472040 0.54797804 5.3130198 9
AAGCA 501225 0.5470992 7.092438 70-79
AAGAA 391565 0.53462315 5.3827353 8
GAAGG 763320 0.5321261 5.4347124 10-14
GAAAA 388330 0.5302062 6.0156126 8
ACGAT 549055 0.5095534 7.139911 10-14
TTGCC 800215 0.50478977 5.719139 60-69
AAGGG 718325 0.5007592 6.0661144 10-14
TGATC 620410 0.48954564 5.491014 240-247
ATAAG 410475 0.47650898 5.5638976 9
GGTCG 1005405 0.47640795 5.473696 70-79
GATCC 631675 0.4686591 5.515466 240-247
TACCG 609550 0.4522439 5.5193543 70-79
GAAGC 575360 0.44875062 5.524324 30-34
CGGTT 770105 0.43420655 7.8286796 10-14
TAAGG 522065 0.43305296 5.7976937 10-14
AAGCT 466235 0.43269184 9.365561 30-34
TAAAG 341735 0.39671063 5.5820165 9