FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_8_SL248354.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_8_SL248354.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10581761
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12032040 8.916646 62.69351 110-119
ATACA 11074860 7.7368984 38.003414 110-119
CACAG 16106695 7.588182 42.457832 110-119
TCCCT 18807565 7.1309724 104.24592 30-34
CAGTA 13513890 6.753755 39.572155 110-119
AGTAA 10494035 6.5572658 48.30951 110-119
GGAGA 16825680 6.3417225 95.9703 35-39
GTAAT 11347265 6.01575 40.18765 110-119
GGTGA 18613885 5.95237 102.05279 45-49
CCCTG 18476445 5.906806 68.10808 30-34
AATAC 7967055 5.5657854 40.097935 110-119
AGACG 13082915 5.5129933 103.55803 40-44
GTAGT 14168750 5.373614 30.747118 80-89
ACAGT 10716315 5.355628 36.049328 110-119
GTGAC 14316330 5.1183853 85.467384 45-49
GAGAC 11661755 4.914132 143.5517 40-44
CCCCA 11590060 4.8825874 102.458 60-69
TAGTA 9013665 4.778592 39.81242 80-89
CTCAG 11814725 4.7225137 34.952637 140-149
ACTCC 10419960 4.656547 117.73722 30-34
ACCAG 9644455 4.543693 92.65819 45-49
GGCCC 16845240 4.5407696 58.891853 60-69
GCACA 9429570 4.4424562 34.400005 110-119
TGACC 11025910 4.407213 146.76094 45-49
GTGTC 14470655 4.389426 24.016315 120-129
AGTAG 9770090 4.3673215 32.02133 80-89
CGTGT 14386930 4.3640285 23.52078 120-129
CACTC 9611240 4.2951407 96.521065 25-29
CTCCC 11580005 4.1389613 67.50921 30-34
CCCAG 10827355 4.0797954 87.504166 60-69
ATGCA 8066690 4.0314407 94.62197 25-29
GACCA 8550470 4.028295 106.01362 45-49
TGAGG 12018700 3.843354 69.82149 35-39
AGGAG 10155745 3.8277745 96.30821 35-39
CCCTT 10077325 3.8208632 39.991356 20-24
ACGGT 10545645 3.770287 97.97724 40-44
TACAC 6742285 3.7672153 27.420687 110-119
GACGG 12442065 3.7506952 109.78268 40-44
ATGGT 9813060 3.7216828 26.218412 190-199
AGATA 5954320 3.7205954 20.34601 150-159
CTGAG 10391140 3.7150478 105.44741 35-39
TGTCC 10793410 3.6603832 20.87673 120-129
CAGGG 12082105 3.6421843 34.737476 50-59
CCACT 8092290 3.6163414 28.527296 240-247
GAGGA 9591185 3.6149879 126.57769 35-39
TGCAC 8848770 3.5369792 113.24405 25-29
GCCCC 11657590 3.513253 73.28538 60-69
TGGTG 12770975 3.4649363 18.12694 190-199
CTTGG 11371675 3.449403 22.82537 20-24
AGGCT 9643925 3.4479034 28.637993 30-34
CCGTG 11926055 3.4102228 23.499685 120-129
GGGTT 12452400 3.3785028 41.123173 50-59
CCTGA 8438500 3.3729885 82.98741 30-34
GGATG 10481215 3.351696 68.73034 20-24
CTCTC 8789590 3.3326128 13.982043 130-139
TCTTG 9238370 3.3235087 18.33244 170-179
CAGAT 6632265 3.3145669 18.377235 150-159
CCAGG 9771880 3.2934096 39.484604 50-59
TCAGG 9193210 3.2867634 21.09321 140-149
GCACT 8144130 3.2553241 87.60641 25-29
CTTCA 6830580 3.2380917 17.442278 200-209
TCGCA 8004475 3.1995025 24.227013 100-109
TCTCA 6715925 3.1837387 22.295347 130-139
CCTGG 11047385 3.1589694 29.003923 50-59
TGTCT 8760550 3.1516132 19.92308 180-189
CGCAC 8247100 3.1075435 24.717743 100-109
ACAGA 5214775 3.0717242 14.379114 140-149
CGGAT 8489505 3.035174 78.08524 20-24
GGTTG 11007145 2.9863856 71.81958 15-19
TTCCC 7853300 2.9776137 48.742214 50-59
GCCCT 9249370 2.9569664 21.263147 20-24
TGGCC 10332640 2.9545898 43.168457 60-69
GATGC 8163610 2.9186602 94.04649 25-29
TCTCT 7201565 2.896521 15.558341 180-189
GGTTC 9546005 2.8956175 46.454952 50-59
AGGGT 8978320 2.8710978 48.962887 50-59
GATGG 8977220 2.870746 21.915901 15-19
TCAAA 4049045 2.8286633 57.50033 70-79
CGGTG 11032285 2.821649 68.81797 40-44
ACCAC 5352485 2.8192601 19.705673 230-239
GACTC 7032065 2.8108165 19.293373 190-199
GTTGG 10212205 2.7707076 51.77948 15-19
GCCGT 9670335 2.7652056 22.193018 120-129
TTCTT 6459430 2.7559903 20.720161 170-179
CGGCC 10140270 2.733391 12.470365 120-129
TCACA 4891365 2.7330236 11.612348 140-149
AGATG 6012800 2.6877778 17.839233 180-189
AGTCA 5371250 2.6843572 30.764076 70-79
GTTCC 7867475 2.668107 52.641304 50-59
TCCAT 5616325 2.6624644 19.741837 70-79
CCAGT 6637145 2.6529613 35.36422 60-69
TGGAG 8164015 2.6106982 19.37865 25-29
GGCTG 10168775 2.6007955 14.808026 30-34
TGTTC 7080305 2.5471437 13.383937 140-149
TCCTC 6709105 2.5437875 17.210337 130-139
GCGGA 8287465 2.498279 85.353424 20-24
AGGGA 6622040 2.4958954 9.046581 150-159
ACAGC 5294105 2.4941573 11.505734 120-129
GAGAT 5513080 2.4643984 17.354513 180-189
AATAA 2789505 2.4365265 10.584199 110-119
ACTCT 5066045 2.4015996 13.6631775 200-209
GGCGG 11133425 2.4009569 41.02952 20-24
GTTCT 6595660 2.3727927 17.806738 160-169
CTGGA 6618775 2.3663492 15.076079 180-189
CATTT 4677965 2.3524594 18.321198 150-159
TTGGG 8622280 2.3393397 77.83649 15-19
TCATT 4637355 2.3320374 16.956884 150-159
CACGG 6913035 2.329895 9.525 120-129
CTGGC 8140030 2.3276188 33.991215 60-69
CAGCT 5769270 2.3060591 11.941238 150-159
TTCAT 4573985 2.30017 16.62693 140-149
TACCA 4107265 2.294912 16.39865 230-239
TGTAG 6036105 2.289242 8.044748 210-219
GTGAA 5048155 2.2565725 18.546755 190-199
CAGAG 5296780 2.2320035 9.288074 140-149
GATAC 4446455 2.222178 15.167126 150-159
TTGTC 6145095 2.2107015 13.653997 170-179
TTCAC 4662025 2.2100706 19.292442 200-209
ATTTG 4864620 2.188093 16.100384 150-159
GAATC 4371640 2.184788 15.156018 190-199
ACTAC 3854075 2.1534436 12.96892 230-239
TCAGC 5370545 2.1466832 14.126354 140-149
CTACT 4505645 2.1359375 7.944237 230-239
TGAAT 4024830 2.1337628 14.670868 190-199
TACAG 4222110 2.1100585 14.118693 160-169
CTCGC 6591625 2.107302 16.451124 100-109
ATGTA 3952960 2.0956607 8.841943 70-79
CACTA 3749975 2.0952783 15.532411 240-247
GTCCT 6136820 2.0811877 14.524462 130-139
GCTGT 6846850 2.0768745 10.357861 140-149
CTCTG 6122575 2.076357 14.869334 180-189
GTCTC 6111315 2.0725384 12.186829 180-189
TTGGA 5445895 2.0654 15.735137 170-179
GTTCA 4855380 2.0587635 15.487888 140-149
TCTGC 6067770 2.0577707 12.35124 210-219
TAGTC 4852385 2.0574934 25.32973 70-79
TGGGG 8984865 2.0554206 51.14295 15-19
ATATA 2773170 2.0551274 10.9256315 110-119
CAGGC 6095840 2.0544765 15.510901 140-149
TCTCG 6041315 2.0487988 15.98638 100-109
ACACA 3109985 2.04811 14.128566 110-119
ATCTC 4285340 2.0315 8.08567 100-109
TTGCA 4777190 2.0256095 14.541784 150-159
AATCG 4037210 2.017652 13.80665 190-199
GAACT 4033810 2.0159528 10.8072815 160-169
AATAT 2718485 2.0146017 13.57443 110-119
AGCTC 5032000 2.011362 12.197072 140-149
TTTGC 5559850 2.0001593 12.339787 150-159
GTCAA 3979520 1.9888208 39.53336 70-79
ACGGC 5873345 1.9794892 14.360899 120-129
TGGAA 4421360 1.9763893 9.308852 170-179
AACCA 2970015 1.9559314 14.727355 70-79
GATAT 3689370 1.9559186 8.209568 180-189
CCAGA 4136025 1.9485631 29.691847 60-69
CCTTC 5120470 1.9414493 14.3182535 200-209
GAGTC 5413560 1.9354601 12.752419 210-219
ACACG 4098770 1.9310114 13.280247 120-129
TCGGC 6714095 1.9198767 7.8877916 130-139
CAGCC 5090955 1.9182942 10.429048 120-129
ATATG 3583405 1.8997414 8.476556 180-189
TACTA 3170420 1.8791598 10.6310215 230-239
GACGT 5240805 1.8736967 9.640591 170-179
CATAG 3735760 1.8669983 8.56368 210-219
CCTTG 5436960 1.8438433 25.96072 20-24
GGGGC 8503075 1.833714 46.542618 15-19
CTGTT 5090250 1.8312203 13.03277 140-149
AGTAT 3412450 1.8091094 12.4452715 220-229
GGGCG 8306545 1.7913316 57.133717 20-24
GTTGT 5532135 1.7801062 7.0860066 210-219
GCAGA 4212530 1.7751129 14.778523 150-159
CGTAG 4932570 1.763496 10.045609 210-219
AGAAC 2985820 1.758775 11.458951 160-169
ATTGT 3884680 1.7473186 8.129203 50-59
TGCAG 4877580 1.7438362 13.689779 150-159
AGTCT 4044245 1.7148284 13.504812 210-219
GGTCA 4738150 1.6939869 7.587963 130-139
GTCCC 5291080 1.6915258 22.117712 50-59
CTCCA 3761760 1.6810825 7.622385 150-159
GTCAC 4194630 1.6766533 8.441408 130-139
ATAGT 3161130 1.6758724 7.562375 220-229
TCAGA 3327885 1.6631571 10.21251 130-139
CAAAA 2019610 1.662945 22.068743 70-79
CACCA 3153105 1.660803 13.682879 230-239
TTGGT 5135160 1.6523693 27.217245 25-29
TGACT 3893335 1.65084 11.63256 190-199
AACTG 3293580 1.6460127 10.719755 160-169
TCCCA 3651750 1.6319203 11.150047 240-247
GAGAA 3086265 1.6260412 10.580341 160-169
TGGTT 5049110 1.6246805 17.718222 10-14
AATAG 2596225 1.6222682 9.79603 240-247
TCTGG 5316850 1.6127754 12.162557 180-189
TTCAG 3765735 1.5967354 9.294073 150-159
CTGTG 5215580 1.5820568 5.3513227 210-219
CAGAC 3354785 1.5805054 17.94718 60-69
GAACC 3340830 1.573931 12.144245 70-79
GTCTG 5187580 1.5735636 9.260484 210-219
ATAGA 2514445 1.5711673 10.4106865 240-247
GCTCC 4890825 1.5635666 6.128743 150-159
ACCAT 2792940 1.56054 12.492743 45-49
ACTGA 3114565 1.5565474 8.647803 180-189
GGCCG 6426650 1.5494893 12.887711 120-129
TGTGC 5102525 1.5477638 8.197182 220-229
TGCTC 4552050 1.5437427 5.308019 150-159
GCTGA 4311265 1.5413666 24.338463 30-34
CTGGT 5079250 1.5407034 6.913894 160-169
TTGTA 3414970 1.5360444 9.066683 210-219
CAAAG 2607215 1.5357605 28.103502 70-79
GCTCA 3824275 1.5286171 10.906645 140-149
TAGTT 3377685 1.5192735 9.75649 220-229
GGACG 5031165 1.5166589 7.13108 170-179
GTGCT 4989570 1.5135007 8.064323 220-229
CCTCG 4727540 1.5113654 9.761519 130-139
GCTCT 4381640 1.4859512 10.0886345 130-139
CCCAC 3513415 1.4801095 10.697489 240-247
CCACC 3507270 1.4775207 9.740637 230-239
CATGT 3484495 1.477485 8.790769 70-79
AAATA 1670710 1.4593017 5.0626326 9
CGACT 3553855 1.4205263 8.5422 190-199
ACCGT 3540470 1.415176 21.080202 45-49
AATTG 2654400 1.4072295 9.417661 170-179
GTGGA 4398110 1.4064327 24.400316 25-29
TATAC 2361370 1.3996226 6.892655 110-119
TCGAC 3486200 1.3934838 9.278408 190-199
TGATA 2608015 1.3826386 7.683917 180-189
CCTCA 3081920 1.3772706 7.407442 130-139
TGGAC 3847885 1.3756988 10.212756 170-179
CACTG 3437885 1.3741715 7.3381615 230-239
CTCTT 3409515 1.3713316 8.064178 200-209
AAAAA 1326340 1.3654649 8.086675 8
GCGTA 3775585 1.3498499 10.659413 210-219
GTCCA 3376625 1.3496851 18.411095 70-79
CCATG 3364525 1.3448486 8.891486 70-79
CCATC 2995130 1.3384855 11.329729 240-247
ACGGA 3175780 1.338238 9.740271 200-209
GGGAG 4935975 1.3308953 6.4454513 150-159
GTAGG 4125620 1.3192956 5.106088 160-169
GAATT 2482500 1.3160968 10.375541 170-179
CTTGA 3098855 1.3139671 8.338744 200-209
AGATC 2622465 1.3106134 6.9289703 130-139
ACTTC 2758125 1.3075114 6.9717665 220-229
AGCCG 3876355 1.306445 9.314227 120-129
CTCGA 3268285 1.30638 8.2642355 190-199
CTGCT 3851065 1.3060166 5.518987 90-99
TATGG 3433410 1.3021487 6.165997 180-189
GAGGG 4813145 1.2977763 8.051268 200-209
CTGAT 3040245 1.2891153 7.2607884 180-189
GGAGG 4774120 1.2872541 18.282536 30-34
GTGTT 3984805 1.2822131 7.8287063 160-169
TCCAC 2849025 1.2731929 9.6302805 240-247
ATCGG 3560340 1.2728955 8.238729 190-199
TTGGC 4183105 1.2688735 8.788073 60-69
TGGTC 4157860 1.2612158 13.583502 50-59
GGCTC 4387570 1.2546136 8.383887 130-139
ACGTG 3504705 1.2530047 9.272215 170-179
AAGTA 2003495 1.251897 15.721402 70-79
GGAGT 3913675 1.2515197 7.205807 210-219
GTGGT 4606610 1.2498349 11.999526 50-59
ATAAA 1403120 1.225572 5.5830016 7
ACTAT 2067085 1.2251952 6.46316 230-239
GATCT 2885130 1.223344 5.5383873 130-139
ATAAC 1750830 1.22313 7.3491797 80-89
GGAAT 2730640 1.2206217 8.648255 170-179
AAAGT 1928560 1.2050734 20.579462 70-79
ACTCG 3001660 1.1998062 8.500032 190-199
TGCCC 3752535 1.1996624 7.579002 60-69
GCATA 2351630 1.1752598 8.23546 210-219
ACTGG 3285960 1.174799 7.0886955 160-169
CCATA 2098265 1.1723942 11.40233 70-79
TTGAG 3086850 1.170713 8.045194 200-209
ATACC 2090120 1.1678432 8.267632 70-79
TTCCA 2435175 1.1544144 7.7151985 230-239
TATCA 1940500 1.150166 7.181191 70-79
CAATA 1644080 1.1485544 6.7736597 100-109
AGCTT 2703530 1.1463425 6.0816793 150-159
ACTGT 2702380 1.145855 5.6276107 230-239
TCACG 2858825 1.1427131 9.861652 200-209
AGTTC 2690590 1.1408557 5.6085625 160-169
ATGGG 3539895 1.1319917 34.644684 15-19
AGAGC 2639735 1.1123546 6.839218 140-149
AAAAG 1508505 1.1109865 14.919018 70-79
AGGTT 2918920 1.1070242 22.936422 10-14
TCTGT 3067985 1.1037095 6.527612 210-219
CGGCT 3846455 1.0998832 7.8634467 130-139
CTCGG 3844440 1.0993071 8.324091 130-139
GAAGA 2077430 1.0945226 19.079082 35-39
AGAGA 2058450 1.0845227 14.118232 35-39
GTCTA 2542430 1.0780334 7.3592353 180-189
ATATC 1809770 1.0726804 7.63591 70-79
TCTAC 2261470 1.0720682 7.156344 180-189
ATCCA 1892660 1.0575135 15.793245 240-247
CTGAA 2097015 1.0480125 15.421989 35-39
TATGT 2325960 1.0462105 7.7414975 220-229
GAGCT 2921815 1.0446095 6.1306715 140-149
GCTTC 3062105 1.0384556 5.3589034 150-159
AACTT 1751495 1.0381398 6.111179 200-209
TACTG 2446230 1.0372429 6.246361 180-189
CTGCC 3211265 1.0266216 5.8521156 200-209
CTGCG 3573375 1.0217967 7.031624 210-219
CATGG 2840385 1.0154966 5.3511477 190-199
GCGGT 3954810 1.0114936 5.723357 130-139
ATACG 2008665 1.0038584 5.1845503 120-129
TCCCC 2782235 0.994435 5.3055663 80-89
AAAAT 1136445 0.99264145 5.1405077 8
CTACC 2214535 0.98964745 8.475929 230-239
TAGAT 1861475 0.9868605 8.025304 240-247
TATAA 1323565 0.9808611 6.7565937 240-247
CGAAC 2075275 0.97770303 12.734908 70-79
ACAAT 1394760 0.9743795 7.759967 100-109
TGCGT 3209910 0.9736714 8.44735 210-219
CGTGG 3791665 0.96976715 8.245181 50-59
CTTCT 2389535 0.9610882 5.421693 210-219
CCATT 2019720 0.9574647 6.118419 50-59
CAGCG 2839110 0.9568631 6.2737527 160-169
GGGGT 4179535 0.9561305 7.3909974 10-14
ACGTC 2374795 0.9492394 12.580667 70-79
TAGGC 2644555 0.9454833 5.729521 160-169
AAGAG 1789200 0.9426647 14.269906 35-39
CGGAG 3125345 0.9421438 6.724345 210-219
GAGTT 2482715 0.94158983 5.3635244 160-169
TGAAG 2089350 0.933959 10.310443 35-39
GAGGC 3090245 0.9315629 15.676103 30-34
ACCCA 1765095 0.92971057 13.179011 240-247
CCCAA 1731930 0.9122419 9.738821 240-247
GGTGG 3971800 0.90860796 24.110611 25-29
TCGAA 1803575 0.90136176 16.501532 70-79
TGTAA 1694980 0.8985933 6.8265295 230-239
TGCAT 2106490 0.89318746 6.2590623 210-219
ACTAA 1268185 0.8859542 12.637679 240-247
ATCAA 1260205 0.8803794 13.704978 70-79
CATCA 1571380 0.878 5.9800158 230-239
GGGCC 3634140 0.87620467 18.22221 15-19
CGTCC 2653800 0.8484035 10.255258 70-79
TTCTG 2356450 0.8477342 5.4063025 210-219
CCAAT 1508030 0.84260356 10.662271 240-247
AGCGT 2350450 0.8403347 6.4914036 160-169
CGGTC 2932925 0.8386618 5.4452963 130-139
GACCG 2465760 0.83103323 19.353561 45-49
TGAAC 1662100 0.8306578 6.466592 190-199
TGGGC 3241960 0.8291731 19.780941 15-19
CCAGC 2183510 0.82275623 5.618432 80-89
GGTCC 2841515 0.81252337 13.690989 50-59
GTATG 2097650 0.7955508 5.350242 220-229
CTTCC 2081920 0.7893693 5.371023 230-239
TAACC 1408580 0.7870364 5.897121 80-89
AACCG 1657275 0.78077495 5.820698 190-199
TGAGA 1696050 0.7581502 6.0297227 240-247
AAAGG 1415905 0.7459891 8.652567 10-14
AGCCA 1572705 0.7409323 5.903144 80-89
CATAC 1325395 0.74055725 9.853205 70-79
AAAGA 996695 0.7340478 5.695589 8
GTCGA 2018595 0.72168964 11.542275 70-79
CCTTT 1784490 0.7177348 5.189141 50-59
CTAAT 1204940 0.7141877 9.830772 240-247
AATCC 1277310 0.71369004 9.4195175 240-247
GTAGC 1981235 0.70833266 5.512179 80-89
ATGAT 1324455 0.70215946 6.8968897 240-247
AAAGC 1185680 0.698416 10.150339 70-79
AAGGT 1536410 0.68678963 9.694328 10-14
TCCAG 1711865 0.6842568 5.0415053 240-247
TGTTA 1516070 0.6819242 5.026922 230-239
CATTG 1597005 0.67715716 5.60275 50-59
CGATG 1864770 0.6666939 15.139839 15-19
AGGAT 1483605 0.6631853 6.6766424 10-14
GGAAG 1732945 0.65315974 6.630044 30-34
TAGGT 1705880 0.64696884 5.3703537 10-14
CCCAT 1441395 0.6441411 5.012729 240-247
CCGTA 1588320 0.6348741 5.4244704 80-89
AGAAG 1198765 0.6315858 5.928798 9
GAGGT 1969265 0.62973386 6.7202406 10-14
TATGA 1173405 0.6220804 5.78533 240-247
AGACC 1301155 0.6129998 5.11601 240-247
CAATC 1093695 0.61109614 9.1414795 240-247
ATAGG 1362880 0.6092201 5.135118 10-14
AATGG 1275130 0.56999505 6.5446253 10-14
CATCC 1272725 0.56876457 7.587174 240-247
AAATG 895435 0.5595184 5.418277 9
TAAAA 634005 0.55377924 5.745521 8
GGGTC 2135225 0.5461114 5.4754267 70-79
AAGAA 720225 0.53043264 5.3315887 8
GAAGG 1406365 0.53006923 5.5728364 10-14
AAGCA 898620 0.5293254 6.810795 70-79
GAAAA 695870 0.5124956 6.115384 8
AAGGG 1344910 0.50690645 6.223496 10-14
ACGAT 1001165 0.5003462 6.890075 10-14
TTGCC 1425815 0.4835385 5.362964 60-69
TGATC 1140060 0.4834048 5.422098 240-247
ATAAG 763745 0.47723106 5.3198447 9
GGTCG 1860350 0.4758085 5.5371547 70-79
GATCC 1162335 0.46460184 5.4586377 240-247
CGGTT 1453740 0.44096714 7.9719257 10-14
GAAGC 1044290 0.44005206 5.367379 30-34
TAAGG 968935 0.433123 5.818317 10-14
TACCG 1080355 0.43183327 5.177244 70-79
AAGCT 846890 0.4232451 9.052952 30-34
TAAAG 642470 0.4014516 5.5197144 9