FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_15_SL248344.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_15_SL248344.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9469751
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10592600 8.687392 60.535885 110-119
ATACA 9690000 7.515237 36.572292 110-119
CACAG 14144160 7.475281 41.680435 110-119
TCCCT 16624060 7.2022243 105.20057 30-34
CAGTA 11970400 6.6900134 38.825428 110-119
AGTAA 9236905 6.386024 46.97839 110-119
GGAGA 14777165 6.2060013 91.63137 35-39
CCCTG 16476425 6.0174117 68.42856 30-34
GTAAT 10052460 5.940987 39.23718 110-119
GGTGA 16408815 5.8908796 100.90346 45-49
AATAC 6990995 5.4219804 38.64292 110-119
AGACG 11287945 5.3180256 99.38055 40-44
GTAGT 12355890 5.2621074 28.378939 80-89
ACAGT 9322970 5.2104187 35.276543 110-119
GTGAC 12532250 5.047157 83.36404 45-49
GAGAC 10372515 4.8867445 138.79227 40-44
ACTCC 9508560 4.8190575 119.810356 30-34
CCCCA 10039500 4.8116217 100.50259 60-69
CTCAG 10364135 4.6823688 35.03336 140-149
ACCAG 8785435 4.64316 91.16846 45-49
TAGTA 7679150 4.538365 36.172394 80-89
GCACA 8504105 4.494475 33.913082 110-119
GGCCC 14579825 4.488656 58.453503 60-69
TGACC 9658925 4.363765 142.26605 45-49
GTGTC 12664730 4.360094 23.650568 120-129
CACTC 8598170 4.3576603 96.679054 25-29
CGTGT 12637730 4.350798 23.131304 120-129
AGTAG 8496695 4.2330513 29.179222 80-89
CTCCC 10268245 4.2068577 68.643776 30-34
GACCA 7684255 4.061179 103.52444 45-49
ATGCA 7266465 4.0610795 94.057365 25-29
CCCAG 9429340 4.028523 85.61496 60-69
TGAGG 10629305 3.815995 67.81532 35-39
AGGAG 8909795 3.741868 91.834915 35-39
ACGGT 9224180 3.714886 94.30941 40-44
TACAC 5912505 3.7068467 26.69777 110-119
CAGGG 10882945 3.6947186 35.03145 50-59
GACGG 10850010 3.6835372 105.57776 40-44
CCCTT 8484180 3.6756942 36.197266 20-24
CTGAG 9112395 3.6698666 103.95219 35-39
ATGGT 8582015 3.6548953 26.27079 190-199
TGTCC 9397530 3.6293464 20.497608 120-129
CCACT 7135690 3.6164572 28.594646 240-247
TGCAC 7943710 3.5888553 113.32237 25-29
AGATA 5188875 3.5873792 19.470747 150-159
GAGGA 8386260 3.5219977 120.87102 35-39
GCCCC 10085645 3.4832325 72.4302 60-69
GGGTT 11284555 3.463136 41.626205 50-59
CCTGA 7550150 3.4110503 82.45193 30-34
TGGTG 11067200 3.3964314 18.248001 190-199
CCGTG 10316450 3.3586304 23.414204 120-129
CTTCA 6265785 3.3580782 17.456833 200-209
CTTGG 9739965 3.3531833 20.518986 20-24
GGATG 9338845 3.3527107 68.02462 20-24
CCAGG 8742515 3.3295553 39.85979 50-59
CTCTC 7673575 3.324507 13.850194 130-139
TCTTG 8132275 3.3211973 18.504017 170-179
AGGCT 8229450 3.3142748 24.823053 30-34
GCACT 7325410 3.3095155 87.45626 25-29
CAGAT 5852595 3.2708964 18.13191 150-159
TCAGG 8041750 3.238682 21.073065 140-149
CCTGG 9784940 3.1855917 28.919558 50-59
TCGCA 7014680 3.1691327 23.592224 100-109
TGTCT 7699785 3.1445696 20.24167 180-189
TCTCA 5854130 3.1374562 21.735455 130-139
CGCAC 7215025 3.082495 24.115286 100-109
CGGAT 7608550 3.0642173 77.532196 20-24
TTCCC 7034860 3.0477896 49.28636 50-59
GGTTG 9886295 3.0340216 72.87565 15-19
ACAGA 4571445 2.9887507 14.246075 140-149
GGTTC 8622655 2.9685266 47.171066 50-59
GATGC 7357810 2.9632363 95.02836 25-29
TGGCC 9093230 2.9603982 43.231926 60-69
AGGGT 8150750 2.9261763 48.89821 50-59
GCCCT 7877860 2.8771002 19.464495 20-24
TCTCT 6276880 2.8756847 15.623303 180-189
AGTCA 5124645 2.86406 31.658655 70-79
TCACA 4539870 2.846273 11.797546 140-149
GTTGG 9211235 2.826851 52.069874 15-19
TCAAA 3637390 2.821037 57.02336 70-79
GCCGT 8655880 2.8180141 22.134293 120-129
CCAGT 6223540 2.8117068 36.522457 60-69
CGGTG 9673035 2.8072422 66.7275 40-44
ACCAC 4734315 2.8068702 19.972506 230-239
GACTC 6192750 2.7977962 19.505342 190-199
GATGG 7726880 2.774004 19.775331 15-19
TTCTT 5718345 2.7703571 21.053137 170-179
CGGCC 8822685 2.7162187 12.545324 120-129
GTTCC 6993010 2.7007155 53.569138 50-59
AGATG 5261200 2.6211286 17.330399 180-189
TCCAT 4784425 2.5641596 17.898964 70-79
GCGGA 7462675 2.53355 85.07195 20-24
TCCTC 5845175 2.5323691 17.359402 130-139
GGCTG 8707880 2.5271413 12.970195 30-34
TGTTC 6159785 2.515638 13.261241 140-149
TGGAG 6974500 2.5038943 16.958765 25-29
AGGGA 5951585 2.4995015 9.0007 150-159
ACAGC 4647155 2.456052 11.198111 120-129
GGCGG 9969975 2.4390934 41.35892 20-24
GAGAT 4848170 2.415357 16.874842 180-189
AAAAA 2131445 2.3921955 14.126028 120-129
TTGGG 7794890 2.3921869 78.04545 15-19
ACTCT 4442745 2.38104 13.908287 200-209
GTTCT 5826445 2.379503 17.906776 160-169
CTGGC 7270035 2.3668375 34.621635 60-69
CAGCT 5147830 2.3257163 12.312663 150-159
AATAA 2420765 2.3225088 9.887938 110-119
CTGGA 5741055 2.312115 14.928511 180-189
CATTT 4063880 2.3031602 18.234999 150-159
CACGG 6023285 2.2939463 9.489824 120-129
TCATT 4045480 2.2927322 16.918713 150-159
TACCA 3638645 2.2812495 16.636255 230-239
TCAGC 5009445 2.263196 14.362581 140-149
TTCAT 3979035 2.2550752 16.325382 140-149
TGTAG 5277410 2.247535 8.087679 210-219
TTCAC 4157895 2.2283778 19.25384 200-209
GTCAA 3953925 2.2097683 41.862167 70-79
GTGAA 4398215 2.1911895 18.26953 190-199
CAGAG 4641390 2.1866717 9.3287325 140-149
TTGTC 5330580 2.1769934 13.580993 170-179
GATAC 3865045 2.1600952 14.612994 150-159
ATTTG 4261645 2.1530082 15.945238 150-159
CTACT 4000415 2.143978 7.690349 230-239
TCTGC 5535580 2.137853 12.484904 210-219
ACTAC 3404845 2.1346688 12.658262 230-239
GAACT 3807205 2.1277695 11.018921 160-169
ATCTC 3956015 2.1201823 9.359755 100-109
TCTCG 5484490 2.1181219 16.79135 100-109
TGGGG 8139305 2.1056669 51.168995 15-19
CTCGC 5736210 2.094941 16.05099 100-109
CACTA 3330380 2.087983 15.636252 240-247
TAGTC 4364225 2.0850074 26.077465 70-79
GTCTC 5394625 2.0834157 12.304993 180-189
GTCCT 5367955 2.0731158 14.627991 130-139
TTGGA 4865500 2.0721116 15.919705 170-179
GTTCA 4303660 2.0560725 15.329864 140-149
GCTGT 5968975 2.0549424 10.325024 140-149
CTCTG 5315375 2.0528092 14.9644 180-189
TACAG 3671585 2.051974 13.894323 160-169
GAATC 3669255 2.050672 14.814841 190-199
GATAT 3467405 2.0492306 8.179244 180-189
ATATA 2477315 2.0317397 10.477363 110-119
ACACA 2767175 2.0294933 13.821293 110-119
AGCTC 4477795 2.023004 12.106959 140-149
CAGGC 5306750 2.0210562 15.448023 140-149
ACACG 3822780 2.0203643 13.238641 120-129
ATGTA 3406870 2.0134544 7.90378 70-79
TTGCA 4199200 2.006167 14.678861 150-159
TGAAT 3385395 2.0007627 14.201222 190-199
AATAT 2425130 1.988941 13.095566 110-119
AATCG 3541600 1.9793283 13.722863 190-199
TTTGC 4843785 1.9781878 12.524057 150-159
CCTTC 4503435 1.9510725 14.479505 200-209
TGGAA 3901050 1.9435021 9.332233 170-179
AACCA 2649875 1.9434634 14.630924 70-79
ACGGC 5093950 1.9400122 14.259762 120-129
GAGTC 4731385 1.9054872 12.711318 210-219
TCGGC 5831920 1.8986439 7.952045 130-139
CAGCC 4442045 1.8977873 10.223752 120-129
GGGGC 7678810 1.8785739 46.62348 15-19
ATATG 3164285 1.8700869 8.375081 180-189
CCAGA 3538090 1.8699037 26.858566 60-69
TACTA 2790080 1.84977 10.163666 230-239
CATAG 3301695 1.8452502 8.435289 210-219
GGGCG 7481370 1.8302716 58.127 20-24
GACGT 4481155 1.804711 9.72513 170-179
CTGTT 4411720 1.8017336 13.063367 140-149
CTCCA 3540740 1.7944915 7.9716306 150-159
GTCAC 3933055 1.7768983 8.619004 140-149
AGTAT 2991060 1.7677114 12.197101 220-229
GTTGT 4833270 1.7595793 7.122719 210-219
CGTAG 4357095 1.7547479 9.78181 210-219
GCAGA 3711385 1.7485237 14.737552 150-159
CCTTG 4503970 1.739443 23.411947 20-24
TGCAG 4316345 1.7383367 13.777694 150-159
ATTGT 3432260 1.7339981 7.644624 170-179
AGAAC 2650960 1.7331628 11.526071 160-169
TTCAG 3568980 1.7050794 9.372703 150-159
AGTCT 3535480 1.6890746 13.415864 210-219
CACCA 2832805 1.6795073 13.749101 230-239
GTCTG 4844780 1.6679151 9.2891655 210-219
GGTCA 4123275 1.6605809 7.5339804 130-139
ATAGT 2790070 1.6489265 7.5878835 220-229
TGGTT 4512090 1.642652 17.609411 10-14
TGACT 3417465 1.632693 11.739998 190-199
AACTG 2919890 1.6318672 10.777192 160-169
TCAGA 2916290 1.6298552 9.997028 130-139
GAGAA 2757020 1.6067979 10.507763 160-169
CAAAA 1768245 1.6042764 20.812756 70-79
GTCCC 4382720 1.6006281 20.40078 50-59
TCCCA 3157630 1.6003265 10.992919 240-247
TCTGG 4645310 1.5992435 12.225901 180-189
TTGGT 4390375 1.598341 25.024132 25-29
CATGT 3332740 1.5922158 8.914045 70-79
GCTCC 4309360 1.5738361 6.3607745 150-159
AATAG 2274325 1.5723768 9.738531 240-247
CAAAG 2396200 1.566604 28.568731 70-79
TGCTC 4045230 1.5622766 5.410504 150-159
ACTGA 2794225 1.5616356 8.693631 180-189
GAACC 2952100 1.5602041 12.146505 70-79
CTGTG 4502715 1.5501522 5.3950877 210-219
CTGGT 4490185 1.5458387 7.01583 160-169
TTGTA 3055325 1.5435684 9.232318 210-219
GGCCG 5620355 1.5424565 12.900121 120-129
ATAGA 2229055 1.5410789 10.333385 240-247
TGTGC 4467520 1.5380356 8.33312 220-229
TAGTT 3037060 1.5343406 10.098393 220-229
GCTCA 3383295 1.5285243 10.90871 140-149
GTGCT 4417525 1.520824 8.143836 220-229
ACCAT 2399300 1.504242 11.443214 45-49
CAGAC 2845840 1.504045 15.965645 60-69
GGACG 4410505 1.4973497 7.1999097 170-179
CCTCG 4080445 1.4902334 9.759542 130-139
GCTCT 3851980 1.487643 10.140791 130-139
GCTGA 3594950 1.4478066 21.384981 30-34
CCACC 3020220 1.4474981 9.915734 230-239
CCCAC 3011090 1.4431221 10.555312 240-247
CGACT 3143605 1.4202359 8.748445 190-199
AGATC 2525765 1.4115987 6.969512 130-139
AATTG 2378985 1.4059762 9.379253 170-179
TATAC 2110740 1.3993803 6.528997 110-119
CTGCT 3607750 1.3933209 5.6473603 90-99
TCGAC 3068275 1.386203 9.381761 190-199
CACTG 3047795 1.3769504 7.4756083 230-239
CTCTT 2998975 1.373948 8.340154 200-209
TGGAC 3407715 1.3724009 10.348076 170-179
TGATA 2314915 1.3681109 7.561301 180-189
CCTCA 2684785 1.3606827 7.47639 130-139
GCGTA 3355455 1.351354 10.348607 210-219
GTGGA 3747470 1.345368 21.735266 25-29
GGGAG 4444130 1.3449522 6.4397855 150-159
CTTGA 2811390 1.3431408 8.43623 200-209
ATCGG 3327955 1.3402789 8.16596 190-199
GAGGG 4424260 1.338939 8.168068 200-209
GGAAT 2686660 1.3384933 8.759045 170-179
ACCGT 2930885 1.3241322 18.904053 45-49
GAATT 2216165 1.3097498 10.505118 170-179
GTAGG 3647200 1.3093704 5.181993 160-169
ACGGA 2771330 1.305641 9.385945 200-209
CCATC 2574475 1.3047762 11.125806 240-247
CTCGA 2872725 1.2978563 8.414268 190-199
CCATG 2871000 1.297077 7.994743 70-79
CTGAT 2711670 1.2954997 7.3549895 180-189
ACTTC 2406260 1.2896085 6.9841943 220-229
AGCCG 3376355 1.2858726 9.113764 120-129
TATGG 3000655 1.2779142 6.102713 180-189
GTGTT 3475020 1.2651008 7.6426597 160-169
GTCCA 2793135 1.2618986 16.649446 70-79
TCCAC 2474585 1.2541507 9.713708 240-247
GGAGT 3475920 1.2478796 7.2160263 210-219
GGCTC 3823360 1.2447357 8.440084 130-139
ACGTG 3078070 1.2396418 9.264246 170-179
ATAAC 1586515 1.2304474 7.546803 80-89
GGAGG 4062650 1.2295028 16.40596 30-34
AAGTA 1774920 1.2271082 15.205986 70-79
ACTAT 1848970 1.225832 6.356564 230-239
AAAGT 1772845 1.2256737 20.951271 70-79
GATCT 2555825 1.2210448 5.4397635 130-139
TTGGC 3520695 1.2120718 8.201143 60-69
AGAGC 2569665 1.2106317 6.951871 140-149
GTGGT 3929090 1.2058051 10.862746 50-59
ACTCG 2659895 1.2017027 8.55575 190-199
TGGTC 3481650 1.1986297 12.326641 50-59
GAAGA 2048560 1.1939057 18.7839 35-39
ATATC 1784335 1.1829801 7.3136406 70-79
ACTGG 2921805 1.1767087 7.184511 160-169
TTGAG 2732535 1.1637278 8.113263 200-209
ATAAA 1206710 1.1577308 5.322607 7
TGCCC 3157600 1.1531979 6.8896403 60-69
GCATA 2061660 1.1522198 8.098403 210-219
TTCCA 2145305 1.1497525 7.933523 230-239
CTGAA 2053445 1.1476287 14.525482 35-39
AGCTT 2401440 1.1472874 6.19461 150-159
ATACC 1820005 1.1410526 7.485483 70-79
TATCA 1708310 1.1325771 6.8568215 70-79
TCACG 2497890 1.1285112 9.665689 200-209
AGTTC 2354485 1.1248546 5.6248794 160-169
CCATA 1789935 1.1222004 10.430648 70-79
ACTGT 2337445 1.1167139 5.7545767 230-239
AGGTT 2618530 1.1151755 22.681002 10-14
CAATA 1419590 1.1009862 6.4250116 100-109
TCTGT 2664225 1.0880617 6.657977 210-219
CGGCT 3337550 1.086575 7.907739 130-139
CTCGG 3336505 1.0862348 8.3959 130-139
TCTAC 2022090 1.0837168 7.3306017 180-189
GTCTA 2265010 1.0821079 7.4517674 180-189
AAAAG 1326150 1.0725428 13.889359 70-79
ATGGG 2978030 1.0691336 31.016161 15-19
CTTCT 2295805 1.0517982 5.6935353 210-219
GCTTC 2723370 1.0517714 5.5332026 150-159
TACTG 2196385 1.0493224 6.378422 180-189
GAGCT 2593770 1.044598 6.1785426 140-149
ATCCA 1660920 1.0413142 15.640708 240-247
AGAGA 1781550 1.0382917 12.932183 35-39
AAGAG 1781000 1.0379711 13.956004 35-39
GTCCG 3178065 1.034653 5.1196685 130-139
TATGT 2046335 1.0338205 7.6211443 220-229
CTGCG 3167745 1.0312932 7.011038 210-219
CTGCC 2790170 1.0190076 5.977031 200-209
GCGGT 3503110 1.0166487 5.7367597 130-139
AACTT 1519285 1.0072571 6.0357537 210-219
TAGAT 1702565 1.0062131 8.021712 240-247
GGGGT 3853510 0.9969166 7.667055 10-14
CATGG 2473235 0.99605453 5.3611884 190-199
ACGTC 2191450 0.99006593 11.47688 70-79
ATACG 1770945 0.9897451 5.1415176 120-129
CTACC 1952630 0.9896174 8.617661 230-239
CAGCG 2576715 0.9813326 6.3093243 160-169
TGCGT 2818475 0.9703181 8.3719425 210-219
CGAAC 1827045 0.9656052 12.72447 70-79
TCCCC 2340060 0.95871294 5.0595694 80-89
TGAAC 1711380 0.9564555 6.612807 190-199
TATAA 1166085 0.9563504 6.706047 240-247
ACAAT 1209845 0.93831503 7.4635625 100-109
CGGAG 2757725 0.9362371 6.7070026 210-219
CGTGG 3210875 0.93183815 7.519666 50-59
TAGGC 2306205 0.928786 5.832317 160-169
TTCTG 2259525 0.92278343 5.665219 210-219
CCATT 1720525 0.9220963 5.8044868 50-59
GAGTT 2164855 0.9219651 5.3533883 160-169
ACCCA 1536670 0.9110576 13.159069 240-247
TGTAA 1529875 0.9041535 6.830844 230-239
CCCAA 1506470 0.8931525 10.014945 240-247
TCGAA 1583515 0.8849943 16.209902 70-79
TGAAG 1764420 0.8790336 9.506151 35-39
GTATG 2052795 0.8742411 5.322382 220-229
TGCAT 1828295 0.8734675 6.189371 210-219
GGTGG 3372045 0.8723599 21.652542 25-29
GAGGC 2559445 0.868922 13.732542 30-34
ACTAA 1116855 0.86619514 12.6879225 240-247
AGCGT 2132560 0.8588533 6.5124264 160-169
GACCG 2252945 0.85802597 17.452974 45-49
CATCA 1367330 0.857248 5.94612 230-239
CCAGC 1988220 0.8494328 5.4814963 80-89
CGGTC 2575635 0.8385255 5.490495 130-139
CCAAT 1324660 0.830496 10.849449 240-247
GGGCC 3017250 0.8280576 16.482574 15-19
ATCAA 1066750 0.82733524 12.653302 70-79
CAATC 1312415 0.82281905 9.359974 240-247
CGTCC 2192320 0.80066466 9.457917 70-79
CTTCC 1845720 0.7996415 5.6328955 230-239
TAACC 1267455 0.79463124 6.100515 80-89
AACCG 1481085 0.78276306 5.8215055 190-199
TGGGC 2680290 0.77785546 17.847235 15-19
GGTCC 2341730 0.7623752 12.467056 50-59
TGAGA 1520685 0.7576049 5.6993093 240-247
AAAGG 1282740 0.747584 8.643324 10-14
GGAAG 1779800 0.74746686 6.5847845 30-34
AAAGA 919625 0.7437599 5.677497 8
AGCCA 1394540 0.7370235 5.614875 80-89
AGACC 1386005 0.7325127 5.0274 240-247
GTCGA 1785875 0.71923167 11.606812 70-79
CATAC 1128220 0.7073379 8.984217 70-79
CTAAT 1063520 0.7050935 9.932567 240-247
GTAGC 1742440 0.7017389 5.448816 80-89
AATCC 1116005 0.69967973 9.64938 240-247
AAGGT 1401450 0.698202 9.611374 10-14
ATGAT 1180575 0.69771785 7.089239 240-247
AAAGC 1022605 0.6685657 9.51605 70-79
CATTG 1374085 0.6564688 5.293729 50-59
TAGGT 1510520 0.6432979 5.431026 10-14
AGGAT 1286380 0.6408742 6.0015116 10-14
GAAAA 788440 0.6376621 5.7363157 8
CGATG 1540015 0.62021554 13.784667 15-19
GAGGT 1721450 0.6180126 6.6036363 10-14
TATGA 1035225 0.6118162 5.860447 240-247
AGAAG 1049270 0.61151713 5.156787 9
ATAGG 1226135 0.6108601 5.2099166 10-14
CCGTA 1342000 0.60629654 5.064721 80-89
TCAAT 897485 0.59501547 5.793844 70-79
GGGTC 2020400 0.5863467 5.557673 70-79
AATGG 1152060 0.5739561 6.5243325 10-14
AAATG 805175 0.5566656 5.2479 9
CATCC 1075025 0.54483616 7.276852 240-247
TAAAA 558545 0.5358742 6.044142 8
GAAGG 1275945 0.5358617 5.395356 10-14
AAGAA 642650 0.5197524 5.0873013 9
AAGCA 788540 0.515537 6.3902006 70-79
AAGGG 1215110 0.5103127 6.1356416 10-14
GGTCG 1679030 0.4872766 5.658751 70-79
TGATC 992315 0.47407824 5.468358 240-247
GATCC 1020400 0.4610022 5.5176044 240-247
ACGAT 824825 0.46097794 6.0840325 10-14
ATAAG 662140 0.45777693 5.7151675 9
CGGTT 1274580 0.43880036 7.837211 10-14
TAAGG 869505 0.43318713 5.80768 10-14
GAAGC 904380 0.42607543 5.1589675 30-34
AAGCT 740345 0.41376376 8.452158 30-34
TAAAG 566500 0.3916553 5.2190566 9