FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_17_SL248346.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_17_SL248346.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6746810
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7589885 8.774783 61.610714 110-119
ATACA 6929145 7.5731406 37.08733 110-119
CACAG 10097905 7.4973803 42.033867 110-119
TCCCT 11988125 7.2694397 106.373184 30-34
CAGTA 8616130 6.7669864 39.39402 110-119
AGTAA 6624315 6.45121 47.7575 110-119
GGAGA 10561350 6.225947 92.90948 35-39
CCCTG 11965680 6.112039 69.497955 30-34
GTAAT 7203980 5.9849486 39.77254 110-119
GGTGA 11734335 5.901088 100.63668 45-49
AATAC 5012320 5.478165 39.31145 110-119
AGACG 8052170 5.3271513 100.22993 40-44
GTAGT 8819920 5.2654953 27.75614 80-89
ACAGT 6677085 5.244088 35.72189 110-119
GTGAC 9029390 5.095986 83.34106 45-49
GAGAC 7412810 4.9041643 140.39139 40-44
CCCCA 7213435 4.847294 101.4056 60-69
ACTCC 6786655 4.8241167 120.89154 30-34
CTCAG 7458220 4.7239046 35.526985 140-149
ACCAG 6354470 4.717996 93.52015 45-49
TAGTA 5435490 4.5157156 35.386307 80-89
GCACA 6051700 4.493199 34.26066 110-119
GGCCC 10392575 4.4716825 59.102024 60-69
CACTC 6168365 4.3846216 98.06763 25-29
GTGTC 9093900 4.378321 23.810778 120-129
TGACC 6911895 4.377872 142.75105 45-49
CGTGT 9052140 4.3582153 23.352345 120-129
AGTAG 6040110 4.226995 28.441023 80-89
CTCCC 7364580 4.221753 69.01368 30-34
GACCA 5541840 4.1146436 105.78534 45-49
ATGCA 5235620 4.111982 94.831505 25-29
CCCAG 6757155 4.0459914 86.29813 60-69
TGAGG 7614215 3.829118 68.06138 35-39
AGGAG 6404295 3.7753508 93.24279 35-39
ACGGT 6626340 3.7397578 95.48788 40-44
TACAC 4241045 3.73811 26.975836 110-119
CAGGG 7826485 3.7207935 35.549862 50-59
GACGG 7781310 3.6993167 106.703545 40-44
CTGAG 6553535 3.6986685 104.64806 35-39
ATGGT 6149090 3.6710088 26.351137 190-199
CCACT 5125990 3.6436763 28.74333 240-247
TGTCC 6728025 3.6353078 20.706568 120-129
TGCAC 5701560 3.611267 114.464806 25-29
CCCTT 5946270 3.605739 35.251347 20-24
AGATA 3697305 3.6006877 19.778381 150-159
GAGGA 6021445 3.5496595 122.1116 35-39
GCCCC 7244930 3.4984694 72.866104 60-69
GGGTT 8134195 3.4896019 42.288773 50-59
CCTGA 5461765 3.459385 83.963104 30-34
CCAGG 6329530 3.3770416 40.454426 50-59
TGGTG 7868315 3.3755383 18.33738 190-199
GGATG 6700860 3.3698003 69.0312 20-24
GCACT 5275035 3.3411133 88.67015 25-29
CCGTG 7330225 3.3363369 23.618 120-129
AGGCT 5909825 3.3353727 24.44688 30-34
CTTCA 4415585 3.3201306 17.682535 200-209
CTCTC 5464800 3.3137822 13.891078 130-139
TCTTG 5790960 3.309855 18.438503 170-179
CTTGG 6873890 3.3094819 19.881514 20-24
CAGAT 4176220 3.279944 18.328777 150-159
TCAGG 5765675 3.254018 21.295267 140-149
CCTGG 7090935 3.2274244 29.597376 50-59
TCGCA 5054715 3.201567 23.956419 100-109
TCTCA 4184170 3.146127 22.145008 130-139
TGTCT 5491250 3.1385543 20.244995 180-189
CGCAC 5194965 3.110597 24.393486 100-109
CGGAT 5482995 3.0944796 78.66826 20-24
TTCCC 5092755 3.0881789 50.06523 50-59
GGTTG 7096300 3.0443408 73.34193 15-19
GGTTC 6221250 2.995264 47.90818 50-59
GATGC 5298235 2.990205 95.825035 25-29
ACAGA 3246090 2.9885187 14.3675995 140-149
TGGCC 6543210 2.9781287 43.61787 60-69
AGGGT 5873655 2.9538066 49.837723 50-59
TCTCT 4475290 2.87062 15.71112 180-189
ACCAC 3441590 2.8676987 20.53983 230-239
TCAAA 2616580 2.8597653 57.96354 70-79
GCCCT 5595880 2.8583612 18.924261 20-24
AGTCA 3627940 2.8493326 32.083694 70-79
GTTGG 6619965 2.8399913 52.162605 15-19
CCAGT 4464025 2.827434 37.13444 60-69
GACTC 4449980 2.8185384 19.75631 190-199
CGGTG 6921275 2.807001 67.372215 40-44
GCCGT 6161320 2.8043122 22.34394 120-129
TTCTT 4051285 2.7488568 20.976864 170-179
GATGG 5448055 2.7397764 19.29028 15-19
GTTCC 5063535 2.7359452 54.449013 50-59
CGGCC 6340095 2.727995 12.687918 120-129
TCACA 2968635 2.6165922 11.552488 140-149
AGATG 3732960 2.6124034 17.425636 180-189
TCCAT 3404755 2.5600753 17.250202 70-79
GAGAT 3650725 2.5548537 17.105051 180-189
GCGGA 5364740 2.550454 85.83491 20-24
TCCTC 4179060 2.5341265 17.498016 130-139
GGCTG 6238015 2.5298975 12.6169195 30-34
TGTTC 4392530 2.5105746 13.354035 140-149
AGGGA 4214020 2.484177 9.039408 150-159
TGGAG 4908440 2.4684093 16.49497 25-29
ACAGC 3299700 2.4499245 11.2479725 120-129
GGCGG 7169410 2.449283 41.412617 20-24
TTGGG 5595580 2.4005258 78.560135 15-19
ACTCT 3188740 2.397651 13.816143 200-209
CTGGC 5259430 2.3938189 35.100883 60-69
AATAA 1749335 2.3707538 9.9767275 110-119
GTTCT 4134595 2.3631506 17.922009 160-169
CAGCT 3709220 2.349354 12.349707 150-159
TACCA 2659405 2.3440328 17.130335 230-239
CACGG 4363035 2.3278427 9.557639 120-129
CTGGA 4102825 2.3155427 15.028403 180-189
CATTT 2898020 2.3050113 18.239634 150-159
TCATT 2890070 2.2986882 17.109232 150-159
TTCAT 2840585 2.259329 16.571756 140-149
TGTAG 3776195 2.25439 8.081048 210-219
TCAGC 3558525 2.2539067 14.444857 140-149
TTCAC 2946285 2.215346 19.535929 200-209
ACTAC 2482540 2.1881418 12.8868265 230-239
GTGAA 3126060 2.1876826 18.443586 190-199
TTGTC 3810875 2.1781263 13.522582 170-179
CAGAG 3291355 2.1774933 9.3786545 140-149
CTACT 2891625 2.1742468 7.678405 230-239
AAAAA 1358860 2.1587415 13.428633 120-129
ATTTG 3043135 2.156735 16.029764 150-159
GATAC 2736950 2.1495616 14.84743 150-159
TCTGC 3977740 2.1492648 12.786302 210-219
CACTA 2417790 2.1310704 16.00231 240-247
ATCTC 2824290 2.1236167 9.410325 100-109
GAACT 2702475 2.1224856 10.917119 160-169
TAGTC 3157785 2.115696 26.522758 70-79
TGGGG 5847095 2.1130047 51.70245 15-19
TCTCG 3901170 2.1078925 17.042456 100-109
CTCGC 4125525 2.1073077 16.27541 100-109
TTGGA 3488125 2.0824118 16.207417 170-179
GCTGT 4317805 2.0788372 10.426974 140-149
GTCCT 3846335 2.078264 14.783705 130-139
TACAG 2628615 2.064477 13.982924 160-169
CTCTG 3815290 2.0614893 15.040405 180-189
GTCTC 3813930 2.0607548 12.273731 180-189
ATATA 1776045 2.053313 10.761078 110-119
GTTCA 3054995 2.0468278 15.398673 140-149
GAATC 2605455 2.0462873 14.939656 190-199
GTCAA 2603410 2.0446815 40.544765 70-79
AGCTC 3213825 2.03558 12.403097 140-149
CAGGC 3806850 2.0310972 15.835313 140-149
ATGTA 2435690 2.0235312 7.542835 70-79
ACACG 2723985 2.022474 13.305797 120-129
GATAT 2434075 2.0221894 8.21456 180-189
ACACA 1948480 2.0132027 13.69921 110-119
AATAT 1732840 2.003363 13.50187 110-119
AACCA 1933475 1.9976993 15.287349 70-79
TGAAT 2394160 1.9890288 14.236916 190-199
TTGCA 2964255 1.9860321 14.634627 150-159
AATCG 2518670 1.9781276 13.770888 190-199
ACGGC 3696250 1.972088 14.423683 120-129
TGGAA 2801185 1.9603282 9.653139 170-179
TTTGC 3429305 1.960038 12.435762 150-159
CCTTC 3177790 1.9269696 14.616344 200-209
GAGTC 3369955 1.901927 12.781854 210-219
CAGCC 3170630 1.8984828 10.350113 120-129
GGGGC 5524935 1.8874815 47.103336 15-19
TCGGC 4137320 1.8830929 8.030135 130-139
CGTAG 3335790 1.8826451 10.163408 210-219
TACTA 2018715 1.8821709 10.176802 230-239
ATATG 2248875 1.8683285 8.46403 180-189
CATAG 2361760 1.8548927 8.558136 210-219
GGGCG 5388170 1.8407586 58.427284 20-24
CCAGA 2452640 1.8210088 26.153135 60-69
CTGTT 3153075 1.8021575 13.186084 140-149
GACGT 3181195 1.7953951 9.746329 170-179
AGTAT 2138380 1.7765309 12.326529 220-229
CTCCA 2492140 1.7714728 8.104767 150-159
GTCAC 2767225 1.7527113 8.551962 130-139
GTTGT 3430355 1.7470338 7.140692 210-219
AGAAC 1894615 1.7442808 11.393672 160-169
ATTGT 2453955 1.7391704 7.786699 170-179
GCAGA 2618070 1.7320617 14.805878 150-159
TGCAG 3056645 1.7251021 13.800059 150-159
AGTCT 2555460 1.712142 13.737743 210-219
CCTTG 3151890 1.7030389 22.702084 20-24
CACCA 2026015 1.6881735 14.085745 230-239
GGTCA 2984655 1.6844726 7.6265817 130-139
TTCAG 2499145 1.6744113 9.3868265 150-159
ATAGT 2004210 1.6650648 7.7344556 220-229
TGACT 2484555 1.6646363 11.966954 190-199
TCAGA 2110945 1.6579065 10.321137 130-139
GTCTG 3421435 1.6472737 9.473617 210-219
TGGTT 3233475 1.6467655 17.928556 10-14
AACTG 2090705 1.64201 10.795456 160-169
CAAAA 1269330 1.626235 21.09765 70-79
GAGAA 1955255 1.6039939 10.356373 160-169
CTGTG 3325060 1.6008732 5.521286 210-219
GTCCC 3122805 1.595121 19.914747 50-59
GAACC 2148065 1.5948714 12.694332 70-79
TCCCA 2239360 1.5917907 11.137506 240-247
TGTGC 3289770 1.5838826 8.550191 220-229
TGCTC 2931155 1.583771 5.517598 150-159
TTGGT 3105950 1.5818186 24.12135 25-29
CAAAG 1717600 1.5813116 28.982536 70-79
TCTGG 3282650 1.5804546 12.312906 180-189
GCTCC 3093050 1.5799221 6.5345592 150-159
AATAG 1616835 1.5745842 9.702433 240-247
AGATC 1988315 1.5615944 7.174194 130-139
ACTGA 1987105 1.560644 8.775103 180-189
GGCCG 4061830 1.5573041 13.029422 120-129
CTGGT 3232750 1.55643 7.028144 160-169
GCTCA 2442715 1.5471723 11.219909 140-149
TAGTT 2181640 1.5461749 10.237961 220-229
TTGTA 2177025 1.542904 9.166493 210-219
GTGCT 3198975 1.5401688 8.317776 220-229
ATAGA 1569155 1.5281504 10.254755 240-247
ACCAT 1716270 1.5127418 11.497887 45-49
GGACG 3179055 1.5113562 7.267603 170-179
CCTCG 2910110 1.4864768 9.8772955 130-139
GCTCT 2747610 1.4845971 10.241699 130-139
CAGAC 1981540 1.4712318 15.39662 60-69
CCACC 2179995 1.4649161 10.117139 230-239
CCCAC 2143845 1.4406239 10.484013 240-247
CATGT 2147320 1.4386908 7.5864186 70-79
GCTGA 2528085 1.4267945 20.821095 30-34
AAATA 1051380 1.4248636 5.0653687 9
TATAC 1523960 1.4208804 6.8211827 110-119
AATTG 1701285 1.4133995 9.678957 170-179
CGACT 2229385 1.4120529 8.684099 190-199
CTGCT 2569690 1.388463 5.7614923 90-99
TCGAC 2183520 1.3830029 9.387533 190-199
TGGAC 2442340 1.3784021 10.429342 170-179
CTCTT 2144420 1.3755119 8.290072 200-209
GATCT 2048675 1.3725992 5.610987 130-139
CACTG 2166685 1.3723397 7.3809695 230-239
GCGTA 2428315 1.3704865 10.645083 210-219
TGATA 1648475 1.369526 7.7660766 180-189
CCTCA 1925295 1.3685458 7.615637 130-139
GGGAG 3132920 1.3271561 6.4677277 150-159
ACGGA 2004265 1.3259808 9.680149 200-209
CTTGA 1972535 1.321586 8.389809 200-209
GAGGG 3119000 1.3212593 8.128731 200-209
ACTTC 1756040 1.320387 6.9928436 220-229
GTGGA 2622520 1.318841 21.055998 25-29
GAATT 1587255 1.3186654 10.819725 170-179
GGAAT 1873630 1.3112054 8.995392 170-179
CCATC 1841050 1.3086624 11.322407 240-247
CCATG 2063280 1.306845 7.65306 70-79
GTAGG 2596745 1.305879 5.261858 160-169
ATCGG 2307470 1.3022844 8.040028 190-199
CTCGA 2040910 1.2926761 8.398265 190-199
CTGAT 1928505 1.2920862 7.3967767 180-189
ACCGT 2027850 1.2844042 18.02669 45-49
TATGG 2144365 1.2801867 6.1414757 180-189
AGCCG 2384825 1.2723936 9.191928 120-129
TCACG 2007580 1.2715657 10.024995 200-209
GTGTT 2487240 1.266718 7.783255 160-169
ACGTG 2221735 1.2538974 9.370776 170-179
TCCAC 1760745 1.2515796 9.744628 240-247
AAGTA 1280750 1.2472817 15.924107 70-79
GGCTC 2739090 1.2466912 8.513277 130-139
GGAGT 2470280 1.2422808 7.2938137 210-219
GTCCA 1961245 1.2422179 16.152903 70-79
AAAGT 1272240 1.2389942 21.36921 70-79
ATAAC 1132240 1.2374705 7.670865 80-89
ACTAT 1322365 1.2329214 6.31791 230-239
AGAGA 1469395 1.2054185 12.849345 35-39
GGAGG 2845385 1.2053515 15.84832 30-34
ACTCG 1899195 1.2029164 8.60257 190-199
TGGTC 2468855 1.1886474 11.834646 50-59
TTGGC 2467185 1.1878433 8.05215 60-69
AGAGC 1794805 1.1874065 7.0151205 140-149
ATAAA 876150 1.1873863 5.26319 7
ACTGG 2094840 1.1822808 7.2135105 160-169
GTGGT 2751755 1.1805139 10.397527 50-59
TTGAG 1958675 1.1693296 8.078025 200-209
TGCCC 2281610 1.1654407 6.600263 60-69
AGCTT 1733460 1.1614072 6.1311283 150-159
GCATA 1477150 1.1601326 8.268662 210-219
TTCCA 1535200 1.1543348 7.948624 230-239
ATATC 1237345 1.1536521 7.247078 70-79
GAAGA 1404490 1.1521736 18.57385 35-39
TATCA 1228460 1.145368 6.791726 70-79
ATACC 1294505 1.140993 7.3263254 70-79
ACTGT 1675635 1.1226648 5.6847687 230-239
CCATA 1268590 1.1181511 10.200592 70-79
TCTGT 1949970 1.114516 6.848915 210-219
CAATA 1018520 1.1131814 6.6279054 100-109
CTGAA 1415010 1.111329 14.369656 35-39
AGTTC 1656160 1.1096168 5.389665 160-169
AGGTT 1854090 1.1068923 22.83495 10-14
TCTAC 1449915 1.0902082 7.2611017 180-189
CGGCT 2393485 1.0893898 7.9854074 130-139
GTCTA 1625005 1.0887433 7.3711658 180-189
CTCGG 2387715 1.0867636 8.51322 130-139
AAAAG 937535 1.0702866 14.059163 70-79
GCTTC 1966080 1.0623184 5.5158534 150-159
TACTG 1571020 1.0525734 6.4643025 180-189
CTTCT 1638400 1.0509317 5.8360214 210-219
ATGGG 2086920 1.0494927 30.279602 15-19
GAGCT 1859265 1.0493275 6.3090553 140-149
AACTT 1117910 1.0422956 6.2563396 210-219
ATCCA 1180585 1.0405824 15.685606 240-247
CTGCG 2284750 1.0398993 7.1597037 210-219
CTGCC 2029895 1.0368652 6.0962315 200-209
TATGT 1450790 1.0282059 7.7393074 220-229
GTCCG 2250835 1.0244629 5.0474334 130-139
GCGGT 2513475 1.019368 5.787102 130-139
CTACC 1431560 1.0175871 8.889798 230-239
CATGG 1790545 1.0105435 5.446323 190-199
GGGGT 2767030 0.9999406 7.5393205 10-14
ATACG 1269845 0.9973184 5.3135366 120-129
TAGAT 1196970 0.994423 7.878195 240-247
AAGAG 1207505 0.990577 13.877277 35-39
CGAAC 1328835 0.98661864 13.308948 70-79
TGCGT 2047675 0.9858674 8.550646 210-219
AAAAT 724730 0.98217714 5.4391694 8
CAGCG 1838860 0.9811008 6.4741874 160-169
TATAA 846535 0.97869205 6.757112 240-247
ACAAT 875120 0.95645374 7.696213 100-109
AGCTG 1687120 0.9521727 5.118248 90-99
TCCCC 1660760 0.9520324 5.0231886 80-89
TAGGC 1675475 0.9456005 5.9879966 160-169
CGGAG 1972780 0.9378804 6.8414364 210-219
TGAAC 1190315 0.93485653 6.7209654 190-199
ACGTC 1475415 0.93450177 11.087932 70-79
CGTGG 2285115 0.9267541 7.229882 50-59
TTCTG 1617885 0.924711 5.7643013 210-219
CCATT 1219520 0.9169713 5.8131776 50-59
TCGAA 1165515 0.9153791 16.840618 70-79
GAGTT 1524465 0.9101061 5.16162 160-169
AGTTT 1280915 0.9078118 5.1545553 220-229
TGTAA 1083405 0.9000751 6.9357758 230-239
ACCCA 1079685 0.8996457 12.840503 240-247
TGCAT 1329510 0.8907634 6.3574204 210-219
CCCAA 1066875 0.8889717 9.860582 240-247
ACTAA 809540 0.88477874 12.749752 240-247
TGAAG 1259435 0.881379 9.308544 35-39
AGCGT 1525760 0.8611047 6.7155886 160-169
GTATG 1438680 0.8588925 5.4075813 220-229
CCAGC 1427610 0.85481215 5.750417 80-89
CATCA 966700 0.85206145 5.977545 230-239
GAGGC 1787660 0.84987247 13.598269 30-34
CGGTC 1857740 0.84554654 5.534947 130-139
GGTGG 2328085 0.84131604 20.983757 25-29
ATCAA 762125 0.83295697 12.732567 70-79
CCAAT 939810 0.82836026 10.661168 240-247
GACCG 1530120 0.8163764 16.669888 45-49
TAACC 921010 0.81178975 6.2157717 80-89
GGGCC 2106435 0.8076064 15.941 15-19
CTTCC 1307305 0.7927325 5.6732397 230-239
AACCG 1064370 0.79026157 5.968877 190-199
CGTCC 1534065 0.7835965 9.145463 70-79
TGAGA 1089330 0.7623359 5.879451 240-247
TGGGC 1865015 0.7563779 17.292315 15-19
GGTCC 1645840 0.74910074 12.031234 50-59
AAAGG 909690 0.7462644 8.620531 10-14
AGCCA 1000625 0.7429329 5.9375043 80-89
AAAGA 644270 0.7354963 5.497286 8
GTCGA 1301680 0.73463905 11.959744 70-79
GGAAG 1203085 0.70922214 6.394077 30-34
ATGAT 853455 0.70903635 7.2667356 240-247
CTAAT 760180 0.7087621 9.883586 240-247
AGACC 952900 0.7074986 5.0874696 240-247
GTAGC 1241360 0.70059574 5.38677 80-89
CATAC 792980 0.6989425 8.729708 70-79
AAGGT 993405 0.69520557 9.635754 10-14
AATCC 778505 0.68618405 9.489811 240-247
AAAGC 725280 0.66773033 9.550323 70-79
CATTG 983170 0.6587177 5.293329 50-59
AGGAT 901885 0.631158 5.635569 10-14
TAGGT 1055495 0.6301309 5.3474574 10-14
ATAGG 894060 0.62568194 5.1989546 10-14
TATGA 744520 0.618535 5.900167 240-247
GAGGT 1223180 0.6151259 6.664026 10-14
CAATC 691270 0.609294 9.19318 240-247
CGATG 1074580 0.60646886 13.261995 15-19
AGAAG 736165 0.6039131 5.0270767 9
GAAAA 526490 0.6010391 5.9836626 8
GGGTC 1445545 0.5862571 5.7276125 70-79
AATGG 826235 0.5782165 6.519321 10-14
AAATG 568370 0.5535176 5.3030596 9
CATCC 766265 0.5446795 7.555426 240-247
TAAAA 400680 0.5430143 5.7788143 8
GAAGG 893695 0.52683586 5.4131217 10-14
AAGAA 461040 0.5263216 5.191912 9
AAGGG 874485 0.51551145 6.196254 10-14
AAGCA 549210 0.5056312 6.288577 70-79
GGTCG 1221550 0.4954134 5.8096113 70-79
TGATC 725610 0.48615417 5.6101003 240-247
GATCC 725300 0.4593922 5.5455465 240-247
ACGAT 583290 0.4581077 5.9997587 10-14
ATAAG 468290 0.4560528 5.1845164 9
CGGTT 928825 0.4471892 7.962097 10-14
TAAGG 620850 0.43448386 5.720086 10-14
AAGCT 514925 0.4044148 8.137455 30-34
TAAAG 409635 0.39893058 5.7911777 9