FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_2_SL248348.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_2_SL248348.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13380132
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 15391775 9.016754 63.2107 110-119
ATACA 14308505 7.901423 37.994812 110-119
CACAG 20640460 7.678862 43.28418 110-119
TCCCT 24109090 7.185065 106.48644 30-34
CAGTA 17378845 6.8588114 40.222286 110-119
AGTAA 13440745 6.6449013 49.169743 110-119
GGAGA 21569880 6.4318166 96.552826 35-39
GTAAT 14530300 6.083494 40.691452 110-119
GGTGA 23745615 5.9962893 103.21431 45-49
CCCTG 23622880 5.9413667 69.59399 30-34
AATAC 10156615 5.608671 40.110344 110-119
AGACG 16535320 5.5073705 104.26388 40-44
GTAGT 18308850 5.478435 30.920284 80-89
ACAGT 13758385 5.429945 36.62377 110-119
GTGAC 18127115 5.112974 86.10464 45-49
GAGAC 14748260 4.9121594 144.5703 40-44
CCCCA 14684730 4.8713803 102.76019 60-69
TAGTA 11624320 4.866829 39.994015 80-89
CTCAG 15078205 4.7505116 35.21283 140-149
ACTCC 13402505 4.7165294 120.49155 30-34
ACCAG 12298680 4.575473 93.465996 45-49
GGCCC 21363645 4.534533 59.225807 60-69
GCACA 12007235 4.4670463 35.010246 110-119
CGTGT 18560810 4.433586 23.583542 120-129
AGTAG 12512780 4.421158 32.236588 80-89
TGACC 13972845 4.402259 147.33008 45-49
GTGTC 18290070 4.3689146 23.922388 120-129
CACTC 12410825 4.367543 98.47979 25-29
CTCCC 14930600 4.194465 68.8035 30-34
ATGCA 10430075 4.1163797 96.830025 25-29
CCCAG 13774440 4.090856 88.08987 60-69
GACCA 10868910 4.0435557 106.89967 45-49
TGAGG 15323710 3.8695734 70.45489 35-39
AGGAG 12904260 3.8478575 97.06322 35-39
AGATA 7741145 3.8271053 21.017523 150-159
CCCTT 12784660 3.8101234 37.661274 20-24
TACAC 8635545 3.8068306 27.674032 110-119
ACGGT 13394245 3.778011 98.49665 40-44
ATGGT 12577040 3.7633438 26.514547 190-199
GACGG 15746240 3.7482235 110.371704 40-44
CTGAG 13214955 3.72744 106.55248 35-39
CCACT 10459675 3.6809063 29.34136 240-247
CAGGG 15434850 3.6741009 35.334637 50-59
TGTCC 13656950 3.643827 20.909512 120-129
GAGGA 12187970 3.6342707 127.53063 35-39
TGCAC 11364155 3.5803697 115.80809 25-29
GCCCC 14764115 3.500342 73.44316 60-69
TGGTG 16149330 3.4535594 18.3239 190-199
CTTGG 14400420 3.4398012 21.54339 20-24
CCTGA 10900120 3.4341722 84.90103 30-34
GGGTT 16007325 3.4231913 41.831055 50-59
CCGTG 15172935 3.416468 23.710798 120-129
AGGCT 12098630 3.412567 26.992117 30-34
CTCTC 11434275 3.407677 14.438831 130-139
TCTTG 11985315 3.392376 18.792076 170-179
GGATG 13359890 3.3736656 70.30141 20-24
CAGAT 8481900 3.3475041 18.78437 150-159
GCACT 10529425 3.3173811 89.52517 25-29
TCAGG 11747615 3.3135583 21.447012 140-149
CCAGG 12423125 3.3031275 40.00371 50-59
CTTCA 8818195 3.2920525 18.0563 200-209
TCGCA 10314260 3.2495918 24.464115 100-109
TCTCA 8661935 3.233717 22.64274 130-139
TGTCT 11283345 3.1936874 20.442656 180-189
CGCAC 10622535 3.1547754 24.899351 100-109
CCTGG 13917570 3.1337993 29.095522 50-59
CGGAT 10955540 3.0901444 79.85065 20-24
ACAGA 6579790 3.0663855 14.434263 140-149
GGTTG 14178635 3.0321233 73.54242 15-19
TTCCC 10044465 2.9934816 49.14181 50-59
GATGC 10542180 2.973551 96.3494 25-29
TGGCC 13098805 2.9494388 43.546318 60-69
ACCAC 7060155 2.9338474 20.949335 230-239
GCCCT 11650945 2.9303174 20.11112 20-24
TCTCT 9263500 2.9287038 15.875404 180-189
GGTTC 12234225 2.9223664 47.140133 50-59
AGGGT 11482195 2.8995063 49.86864 50-59
TCAAA 5178710 2.8597798 58.556637 70-79
TTCTT 8466240 2.8394969 21.44432 170-179
GATGG 11237845 2.8378026 20.837414 15-19
GTTGG 13212840 2.8255866 52.908634 15-19
CGGTG 13945715 2.811271 69.129875 40-44
GACTC 8896130 2.8027983 19.191372 190-199
CGGCC 13104535 2.7814987 12.743495 120-129
GCCGT 12316820 2.7733605 22.370192 120-129
AGATG 7695480 2.7190545 18.085627 180-189
AGTCA 6833470 2.6969273 31.659634 70-79
CCAGT 8495415 2.6765501 35.76969 60-69
GTTCC 10030070 2.6761348 53.330505 50-59
TCACA 6042295 2.663641 11.651452 140-149
TGTTC 9297395 2.6315753 13.732066 140-149
TCCAT 7006980 2.6158807 19.638927 70-79
TGGAG 10340380 2.6111727 18.266829 25-29
ACAGC 6953580 2.5869377 11.6587105 120-129
TCCTC 8575810 2.5557888 17.466516 130-139
GGCTG 12676890 2.5554929 13.937443 30-34
GCGGA 10719150 2.551579 87.49515 20-24
AGGGA 8436315 2.5155833 9.011529 150-159
GAGAT 7064795 2.4962134 17.640589 180-189
GTTCT 8654255 2.4495385 18.28902 160-169
GGCGG 14268330 2.4273858 41.980686 20-24
CATTT 6086310 2.4104137 18.82755 150-159
TACCA 5436255 2.396479 17.348873 230-239
CTGGA 8492945 2.3955393 15.553763 180-189
AATAA 3449590 2.3862488 10.255726 110-119
ACTCT 6381680 2.3824406 13.493775 200-209
TTGGG 11138120 2.3819044 79.675026 15-19
TCATT 6011305 2.3807087 17.473196 150-159
CACGG 8875700 2.359919 9.554655 120-129
TTCAT 5958200 2.3596773 17.105375 140-149
CAGCT 7382905 2.3260446 12.077593 150-159
CTGGC 10326745 2.3252583 34.352257 60-69
GTGAA 6558470 2.3173127 19.191553 190-199
GATAC 5843430 2.3061938 15.771031 150-159
ACTAC 5165150 2.276967 14.11745 230-239
TGTAG 7594890 2.2725682 8.028781 210-219
ATTTG 6345080 2.249722 16.618168 150-159
TTCAC 6022510 2.2483535 19.821104 200-209
TTGTC 7902525 2.2367651 13.886004 170-179
CTACT 5983975 2.233967 8.525174 230-239
CAGAG 6692735 2.2291296 9.310495 140-149
TACAG 5531720 2.1831732 14.895516 160-169
CACTA 4928885 2.1728137 16.543854 240-247
TCAGC 6791450 2.1397016 14.039001 140-149
GAATC 5408260 2.1344478 15.278991 190-199
CTCGC 8422580 2.1183546 16.422186 100-109
ATGTA 5058950 2.118063 8.767892 70-79
GTTCA 6293620 2.1034966 15.831801 140-149
ATATA 3582150 2.0984821 11.324421 110-119
GTCTC 7863890 2.0981734 12.572911 180-189
TGGGG 11621365 2.0973613 52.332565 15-19
ACACA 4023880 2.0946214 14.42969 110-119
TTGGA 6998395 2.094083 16.237028 170-179
CTCTG 7840570 2.0919518 15.239912 180-189
GTCCT 7831805 2.0896132 14.794791 130-139
TGAAT 4987400 2.0881069 14.793588 190-199
TAGTC 6236200 2.0843053 26.124014 70-79
GCTGT 8720085 2.0829504 10.697071 140-149
CAGGC 7794145 2.0723493 15.832523 140-149
TCTGC 7731185 2.0627663 12.749779 210-219
TTGCA 6141615 2.0526924 15.03544 150-159
AATAT 3498390 2.0494142 13.9512205 110-119
TCTCG 7664720 2.045033 15.758659 100-109
AATCG 5168310 2.0397482 13.93432 190-199
AGCTC 6455875 2.033976 12.105557 140-149
TTTGC 7138800 2.0205972 12.730805 150-159
GTCAA 5096340 2.011344 40.27802 70-79
GAACT 5064260 1.9986832 10.873998 160-169
AACCA 3823210 1.9901631 15.067995 70-79
ACGGC 7484595 1.9900446 14.615277 120-129
TGGAA 5620180 1.9857855 9.87596 170-179
CCTTC 6644125 1.9801021 14.838398 200-209
ATCTC 5300600 1.9788464 7.8581505 100-109
TCGGC 8770010 1.9747305 8.415745 130-139
GAGTC 6985435 1.9703276 13.2389145 210-219
TACTA 4209345 1.9685153 11.271257 230-239
GATAT 4698620 1.9672015 8.2610235 180-189
CAGCC 6541020 1.9426106 10.529713 120-129
ATATG 4616950 1.9330081 8.524851 180-189
CCAGA 5171135 1.9238154 29.409578 60-69
ACACG 5151125 1.916371 13.438408 120-129
AGTAT 4538105 1.8999976 13.388356 220-229
GGGGC 11002745 1.8718312 47.568687 15-19
GACGT 6633025 1.8709257 9.614657 170-179
CATAG 4738910 1.8702793 8.622571 210-219
CTGTT 6582150 1.8630404 13.48902 140-149
GGGCG 10740740 1.827258 58.55952 20-24
GCAGA 5463005 1.8195469 15.318262 150-159
CGTAG 6405790 1.8068316 10.32107 210-219
ATTGT 5054555 1.7921513 8.162778 50-59
GTTGT 7068000 1.7910398 6.9924765 210-219
AGAAC 3823890 1.7820508 11.567423 160-169
AGTCT 5291630 1.7686044 14.158951 210-219
CCTTG 6614510 1.7648252 24.479147 20-24
TGCAG 6238945 1.7597709 13.947586 150-159
ATAGT 4004060 1.6764055 7.694836 220-229
GGTCA 5929990 1.6726264 7.548772 130-139
AACTG 4225155 1.6675181 10.7201605 160-169
CAAAA 2552870 1.6646636 22.1925 70-79
CACCA 4003215 1.663536 13.851563 230-239
GTCCC 6594245 1.6585118 21.885971 50-59
GTCAC 5250325 1.6541578 8.467508 140-149
GAGAA 3945635 1.6462121 10.664535 160-169
CTCCA 4649345 1.6361697 7.461267 150-159
TGACT 4893625 1.6355804 11.554043 190-199
TCTGG 6846005 1.6352923 12.554412 180-189
TGGTT 6450600 1.6345898 18.13125 10-14
GAACC 4370370 1.6259071 12.390027 70-79
TCCCA 4603400 1.6200011 10.979611 240-247
AATAG 3266485 1.6149008 10.328557 240-247
TTGGT 6342305 1.6071478 25.697628 25-29
TCAGA 4053800 1.5998905 9.916526 130-139
ACTGA 4039560 1.5942706 8.825972 180-189
GTCTG 6656760 1.5900877 9.720717 210-219
TTCAG 4674505 1.5623447 9.270181 150-159
CAAAG 3350140 1.5612688 28.878498 70-79
GGCCG 8189280 1.5561705 13.063178 120-129
ACCAT 3522490 1.5528287 12.449774 45-49
TTGTA 4377405 1.5520599 9.005264 210-219
TGTGC 6489265 1.5500786 8.416209 220-229
GCTCT 5806795 1.5493178 10.626343 130-139
GCTCC 6158490 1.5489155 6.01071 150-159
ATAGA 3115865 1.5404366 10.424265 240-247
CAGAC 4140305 1.540316 17.768326 60-69
CCTCG 6121815 1.5396916 10.152996 130-139
GTGCT 6443525 1.5391526 8.369362 220-229
TAGTT 4334720 1.5369254 9.884443 220-229
GCTCA 4874320 1.5356942 10.854999 140-149
CTGGT 6427960 1.5354347 6.8593907 160-169
CTGTG 6377670 1.523422 5.2079396 210-219
TGCTC 5691035 1.5184315 5.13718 150-159
GGACG 6362645 1.5145596 7.1070004 170-179
CCACC 4535625 1.5046074 10.177708 230-239
CCCAC 4472035 1.4835126 10.883081 240-247
GCTGA 5217195 1.4715738 22.916273 30-34
AATTG 3501255 1.4658929 10.007551 170-179
AAATA 2087880 1.4442879 5.133747 9
TATAC 3079460 1.4401206 7.2164416 110-119
CATGT 4300525 1.4373505 8.611157 70-79
CGACT 4493310 1.415654 8.512733 190-199
ACCGT 4475600 1.4100745 20.984262 45-49
TGATA 3365940 1.4092395 7.786497 180-189
TCGAC 4403705 1.3874232 9.173845 190-199
ACGGA 4149005 1.381897 10.076336 200-209
GCGTA 4894095 1.3804395 10.946517 210-219
GAATT 3284930 1.3753227 11.047025 170-179
CACTG 4350930 1.370796 7.4268456 230-239
CTCTT 4315735 1.3644421 8.09352 200-209
TGGAC 4820470 1.3596724 10.084228 170-179
GTGGA 5311485 1.3412666 23.083311 25-29
GGGAG 6281805 1.3387125 6.4450436 150-159
CCTCA 3787295 1.3328023 7.2233963 130-139
GTCCA 4204545 1.3246762 18.285553 70-79
TATGG 4412730 1.3203918 6.1713467 180-189
AGCCG 4955425 1.3175749 9.376597 120-129
CCATC 3740015 1.3161638 11.04369 240-247
CTCGA 4162120 1.3113099 8.223629 190-199
GAGGG 6150645 1.3107611 8.084164 200-209
GTGTT 5158775 1.3072398 8.025451 160-169
CCATG 4142120 1.3050086 8.684613 70-79
GGCTC 5782400 1.3020146 8.880915 130-139
CTGAT 3871000 1.2937918 7.3476915 180-189
ACTTC 3449835 1.2879096 6.8608804 220-229
GGAGT 5081715 1.2832446 7.5688586 210-219
TTGGC 5350025 1.2779504 8.805811 60-69
CTGCT 4782855 1.2761192 5.6592402 90-99
ATCGG 4522370 1.27559 8.37366 190-199
AAGTA 2556785 1.2640359 16.084087 70-79
AGATC 3202785 1.2640252 6.7857013 130-139
GGAAT 3573955 1.2627903 9.25879 170-179
ACGTG 4471830 1.2613344 9.186296 170-179
TCCAC 3565520 1.2547565 9.68285 240-247
CTTGA 3753165 1.2544082 8.32964 200-209
GGAGG 5882785 1.2536775 17.300661 30-34
ATAAC 2269555 1.2532904 7.5991883 80-89
ACTAT 2636865 1.2331394 6.4668913 230-239
GTGGT 5762680 1.2323581 11.894498 50-59
TGGTC 5145680 1.2291389 13.462377 50-59
AAAGT 2471500 1.2218722 21.278044 70-79
ATAAA 1756365 1.214963 5.9401236 7
ACTCG 3846775 1.2119578 8.439856 190-199
GATCT 3596710 1.2021167 5.3530865 130-139
AAAAA 1468165 1.1992502 8.850084 8
ACTGG 4224350 1.1915298 7.0945587 160-169
CCATA 2690555 1.1860845 11.583194 70-79
ATACC 2677485 1.180323 8.428952 70-79
TCACG 3742380 1.1790674 10.129433 200-209
TTGAG 3931125 1.1762843 8.048256 200-209
TGCCC 4656535 1.1711603 7.380579 60-69
AGCTT 3498295 1.1692238 6.2567973 150-159
GCATA 2922705 1.1534876 8.012648 210-219
AGTTC 3445270 1.1515015 5.751553 160-169
TTCCA 3070270 1.1462085 7.780519 230-239
CGGCT 5052390 1.1376394 8.3503895 130-139
TATCA 2425845 1.1344552 7.2743406 70-79
CTCGG 5034110 1.1335233 8.773009 130-139
ACTGT 3359930 1.1229786 5.612283 230-239
AGAGC 3368040 1.1217831 7.045351 140-149
AGGTT 3745445 1.1207246 23.58097 10-14
AAAAG 1911900 1.116136 15.121794 70-79
TCTAC 2963135 1.1062124 7.3616805 180-189
CAATA 1999625 1.10423 6.6232877 100-109
GTCTA 3282335 1.0970442 7.4874344 180-189
GAAGA 2610175 1.0890267 18.736 35-39
TATGT 3071205 1.0889314 8.32086 220-229
ATGGG 4307825 1.0878204 32.869877 15-19
AGAGA 2556840 1.066774 14.113862 35-39
CTACC 3024755 1.0644536 9.26507 230-239
TCTGT 3759965 1.0642369 6.3479357 210-219
GAGCT 3769785 1.0633141 6.272564 140-149
TACTG 3176830 1.0617816 6.4820647 180-189
CTGCG 4699020 1.0580715 7.4543657 210-219
GCTTC 3964845 1.0578648 5.5142403 150-159
ATATC 2257225 1.0555995 7.7199426 70-79
ATCCA 2384200 1.0510333 15.62723 240-247
ATACG 2633550 1.0393686 5.6210585 120-129
CTGAA 2587400 1.0211546 15.372637 35-39
TGCGT 4231935 1.0108744 8.694137 210-219
AACTT 2154325 1.0074778 5.8620296 200-209
CTGCC 3978400 1.0006033 5.6992273 200-209
GCGGT 4958790 0.9996262 5.628325 130-139
TATAA 1701625 0.9968398 6.9016905 240-247
CAGCG 3736710 0.99353665 6.733632 160-169
CGAAC 2660910 0.9899373 12.991276 70-79
CATGG 3503535 0.98821497 5.2579784 190-199
TCCCC 3492370 0.98111427 5.362623 80-89
AAAAT 1414710 0.97862345 5.5768933 8
CGGAG 4103150 0.976711 7.0593514 210-219
TAGAT 2317490 0.97027844 7.9483666 240-247
GGGGT 5372755 0.96964586 7.4638495 10-14
TGCTA 2875545 0.9610841 5.0835905 230-239
GAGTT 3200700 0.95772415 5.5104084 160-169
ATGTG 3190160 0.95457035 5.1934385 220-229
AGCTG 3375040 0.95197123 5.074002 90-99
CGTGG 4708875 0.9492467 8.187832 50-59
ACCCA 2279315 0.9471693 13.513262 240-247
CCATT 2530765 0.9447978 6.128679 50-59
ACAAT 1710325 0.9444732 7.638745 100-109
TGAAG 2667325 0.9424496 10.217093 35-39
ACTAA 1699850 0.9386888 13.6737795 240-247
AAGAG 2214685 0.9240189 14.027521 35-39
ACGTC 2929640 0.92300695 12.495023 70-79
CTTCT 2919360 0.92297083 5.3273387 210-219
TAGGC 3228920 0.91075647 5.5747724 160-169
CCCAA 2182460 0.9069212 9.941193 240-247
TGTAA 2160415 0.90451473 6.894956 230-239
TCGAA 2290035 0.9037953 16.711166 70-79
GAGGC 3695200 0.87960285 14.776455 30-34
TGCAT 2623125 0.8767186 6.146351 210-219
AGCGT 3104860 0.87576365 6.956797 160-169
GGTGG 4828585 0.8714371 22.77312 25-29
ATCAA 1568315 0.86605275 13.779321 70-79
CATCA 1937930 0.8543029 5.809009 230-239
TGAAC 2162865 0.8536058 6.937874 190-199
CCAAT 1906240 0.84033287 10.77287 240-247
CGGTC 3717165 0.8369887 5.409354 130-139
GGGCC 4381690 0.832632 17.20671 15-19
CGTCC 3307155 0.8317792 10.1626 70-79
CCAGC 2789580 0.8284744 5.6657677 80-89
GTATG 2748050 0.8222807 5.7771916 220-229
AACCG 2206510 0.820887 6.153253 190-199
GACCG 3051890 0.8114528 19.218092 45-49
TTCTG 2858215 0.8090017 5.294722 210-219
CTTCC 2676155 0.79755574 5.5211463 230-239
GGTCC 3528655 0.79454213 13.530625 50-59
TAACC 1798105 0.7926635 6.091946 80-89
TGGGC 3896815 0.7855461 18.71646 15-19
AAAGG 1809420 0.75493276 8.934536 10-14
CTAAT 1599990 0.74824107 10.468702 240-247
AGCCA 2001855 0.74474925 5.9493375 80-89
CATAC 1688545 0.7443659 10.023237 70-79
TGAGA 2077030 0.73387986 6.264539 240-247
AAAGA 1253030 0.7314985 5.8061824 8
GTCGA 2532115 0.7142141 11.665263 70-79
AATCC 1617675 0.71312404 9.5055485 240-247
GTAGC 2525375 0.712313 5.6092515 80-89
AAAGC 1501420 0.69970804 10.309745 70-79
ATGAT 1670765 0.6995099 6.8634195 240-247
CCTTT 2208925 0.6983631 5.103377 50-59
AAGGT 1975225 0.6979089 10.024325 10-14
TCCAG 2133805 0.6722727 5.053366 240-247
CATTG 2009740 0.6717089 5.6187005 50-59
CGATG 2369665 0.66839296 14.431782 15-19
TAGGT 2167520 0.64857256 5.4872684 10-14
CCGTA 2032800 0.64045024 5.3965225 80-89
TATGA 1518385 0.635712 5.9405756 240-247
AGGAT 1789375 0.63224226 6.2041755 10-14
GAGGT 2459930 0.6211864 6.904267 10-14
GGAAG 2069435 0.6170746 6.2362523 30-34
CAATC 1398175 0.6163612 9.224753 240-247
AGAAG 1469035 0.6129161 5.78019 9
ATAGG 1705150 0.602483 5.3492503 10-14
AGACC 1614780 0.600746 5.409536 240-247
AATGG 1631125 0.5763276 6.702712 10-14
CATCC 1571140 0.5529062 7.336719 240-247
TAAAA 790415 0.5467684 5.853862 8
GGGTC 2691315 0.54253334 5.546092 70-79
AAATG 1090005 0.538882 5.3938465 9
AAGCA 1145235 0.5337149 6.910357 70-79
AAGAA 903800 0.5276237 5.506967 8
GAAGG 1719145 0.5126233 5.549712 10-14
AAGGG 1702140 0.5075527 6.2715645 10-14
ACGAT 1220760 0.4817905 6.7006364 10-14
GAAAA 823150 0.48054153 5.8125186 8
GGTCG 2366870 0.47712958 5.6335397 70-79
TGATC 1415600 0.47313145 5.2271795 240-247
ATAAG 954260 0.47177175 5.7700505 9
TTGCC 1764590 0.47081235 5.2644715 60-69
GATCC 1432720 0.45139012 5.260456 240-247
TACCG 1398470 0.44059938 5.310238 70-79
CGGTT 1812375 0.43291864 8.016782 10-14
TAAGG 1219405 0.43085405 5.963259 10-14
GAAGC 1273350 0.42411092 5.1327534 30-34
AAGCT 1042905 0.4115975 8.625167 30-34
TAAAG 812980 0.40192503 5.731297 9
GAAAG 878155 0.36638704 5.0581694 9