FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_16_SL248345.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_16_SL248345.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6888018
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7863810 8.91829 62.581654 110-119
ATACA 7170700 7.69217 38.505634 110-119
CACAG 10488460 7.62316 42.968937 110-119
TCCCT 12515110 7.3814416 110.01434 30-34
CAGTA 9015640 6.927574 40.924282 60-69
AGTAA 6832675 6.5286403 49.01194 110-119
GGAGA 10914810 6.294031 92.61941 35-39
CCCTG 12565210 6.2439528 71.188446 30-34
GTAAT 7456360 6.0572124 40.682816 110-119
GGTGA 12073090 5.918956 102.38222 45-49
AATAC 5219745 5.599337 40.559822 110-119
AGACG 8134130 5.2659793 101.67578 40-44
ACAGT 6838975 5.2550354 36.61319 110-119
GTAGT 9014245 5.245327 25.398582 80-89
GTGAC 9276750 5.1059647 84.175285 45-49
ACTCC 7115390 4.9361835 126.85978 30-34
GAGAC 7528670 4.8740087 142.64737 40-44
CCCCA 7367025 4.834186 101.78927 60-69
ACCAG 6639495 4.825678 95.64823 45-49
CTCAG 7623005 4.7104616 35.503513 140-149
CACTC 6533710 4.532653 100.90305 25-29
GCACA 6205830 4.510484 35.123478 110-119
CGTGT 9429975 4.41272 23.525982 120-129
GGCCC 10526710 4.4072323 59.795708 60-69
GTGTC 9413595 4.4050555 24.075151 120-129
TAGTA 5390855 4.3792887 32.21897 80-89
TGACC 7081535 4.3758726 142.74106 45-49
CTCCC 7731420 4.3132486 71.78514 30-34
ATGCA 5533130 4.2516303 99.49479 25-29
AGTAG 6166440 4.220499 29.267262 70-79
GACCA 5780965 4.2016864 107.72852 45-49
CCCAG 6949230 4.0617447 87.00878 60-69
TGAGG 7912885 3.8793724 69.08066 35-39
CAGGG 8302100 3.849934 37.32553 50-59
AGGAG 6573005 3.790327 93.19116 35-39
TACAC 4386915 3.7844186 27.780205 110-119
ACGGT 6824010 3.7559655 95.15439 40-44
CCACT 5400515 3.7465177 29.242504 240-247
TGCAC 6038770 3.73152 119.03055 25-29
ATGGT 6377605 3.7110844 26.691109 190-199
GACGG 7996140 3.7080512 106.89942 40-44
CTGAG 6722265 3.6999643 106.70112 35-39
AGATA 3812660 3.6430078 19.771662 150-159
GGGTT 8683075 3.6192129 44.09081 50-59
TGTCC 6831855 3.5891356 20.4806 120-129
GAGGA 6201500 3.5760984 122.92198 35-39
CCTGA 5767975 3.5641882 86.20822 30-34
CCAGG 6662570 3.4686656 42.41637 50-59
GCCCC 7379255 3.4684951 72.96236 60-69
GCACT 5597170 3.4586432 91.278694 25-29
CCCTT 5804370 3.4234314 31.299732 20-24
GGATG 6959030 3.4117355 70.9373 20-24
TCTTG 6111270 3.3942513 19.082577 170-179
TGGTG 8030855 3.3473594 18.46664 190-199
CTTCA 4534260 3.3255255 17.532042 200-209
CTCTC 5632410 3.3220088 14.031509 100-109
CCGTG 7400225 3.2755141 23.858192 120-129
CCTGG 7385950 3.2691956 30.14185 50-59
CAGAT 4254000 3.268753 18.130241 150-159
TCAGG 5932225 3.2651227 21.128345 140-149
CTTGG 6922910 3.239549 17.618912 20-24
TCGCA 5212115 3.2207074 24.647764 100-109
AGGCT 5828375 3.2079635 21.462723 30-34
TTCCC 5413120 3.1926713 51.90749 50-59
TGTCT 5745480 3.1910884 20.67229 180-189
CGGAT 5797550 3.1909971 80.96155 20-24
GGTTG 7550835 3.1472816 76.670364 15-19
CGCAC 5348655 3.1262271 24.875408 100-109
TCTCA 4238465 3.108583 21.713198 130-139
GGTTC 6618335 3.0970247 49.90493 50-59
GATGC 5605330 3.0851986 100.3966 25-29
AGGGT 6205555 3.0423365 51.994854 50-59
ACAGA 3364070 3.0404348 15.347314 140-149
TGGCC 6788340 3.0046794 44.76276 60-69
TCAAA 2765605 2.9667258 60.468056 70-79
AGTCA 3823135 2.9376786 34.353825 70-79
GTTGG 7042320 2.9353259 54.482655 15-19
CCAGT 4704075 2.9067757 38.36523 60-69
ACCAC 3536530 2.8857317 20.817303 230-239
GACTC 4618380 2.8538225 20.411974 190-199
TCTCT 4564810 2.8463645 15.413213 180-189
TTCTT 4278075 2.820185 21.802084 170-179
GTTCC 5333180 2.8018022 56.701736 50-59
CGGTG 7102870 2.800355 66.89006 40-44
GCCGT 6301370 2.789135 22.71434 120-129
GCCCT 5518320 2.742185 17.057482 20-24
CGGCC 6454820 2.7024484 12.87654 120-129
GATGG 5435170 2.6646473 17.541035 15-19
TCACA 3087400 2.6633782 12.56491 140-149
GCGGA 5701965 2.6441727 89.30398 20-24
AGATG 3825500 2.6182888 17.126482 180-189
TCCAT 3541510 2.597421 16.727173 70-79
AGGGA 4467945 2.576443 9.46184 150-159
GGCGG 7599435 2.5243175 43.24359 20-24
TGTTC 4510590 2.50522 13.115339 140-149
ACAGC 3441855 2.5015888 11.421377 120-129
TCCTC 4212555 2.484575 17.081005 130-139
TTGGG 5949825 2.4799604 81.51959 15-19
CTGGC 5570115 2.465464 36.7598 60-69
GGCTG 6168945 2.432149 11.878458 140-149
GTTCT 4366035 2.424933 18.242058 160-169
ACTCT 3294210 2.416046 14.246925 200-209
CAGCT 3902375 2.4113834 12.814793 150-159
GAGAT 3485580 2.3856373 16.857958 180-189
TGGAG 4850165 2.3778427 14.422085 25-29
TACCA 2733270 2.3578844 17.28188 230-239
AATAA 1759720 2.347352 9.278311 110-119
CACGG 4477155 2.3308954 9.795627 120-129
TCATT 2963690 2.2979891 17.006134 150-159
CATTT 2963605 2.2979233 18.057972 150-159
TCAGC 3703045 2.288212 15.003106 140-149
CTGGA 4140115 2.2787375 14.828429 180-189
TTCAT 2898665 2.2475703 16.314774 140-149
CTACT 3062740 2.2462804 8.072598 180-189
TGTAG 3848495 2.239413 8.565631 210-219
TAGTC 3381920 2.2093368 28.449747 70-79
CAGAG 3410745 2.2080925 10.048287 140-149
ACTAC 2559175 2.2076995 12.928338 230-239
CACTA 2544070 2.1946688 16.190884 240-247
TTCAC 2979595 2.1853004 19.106884 200-209
GTGAA 3185110 2.1799862 18.409678 190-199
TGGGG 6204800 2.1789722 53.04745 15-19
GATAC 2829030 2.1738129 14.74808 150-159
GTCAA 2800620 2.1519828 43.184017 70-79
TTGTC 3855555 2.141408 13.30151 170-179
ATTTG 3094565 2.1372705 15.87677 150-159
TTGGA 3663890 2.1319926 16.785555 170-179
CTCGC 4279925 2.126797 16.726864 100-109
TCTCG 4026900 2.1155438 17.140285 100-109
TCTGC 4004880 2.1039755 12.544077 210-219
GAACT 2725795 2.0944877 11.648711 160-169
AGCTC 3383320 2.0906453 12.947871 140-149
ATATA 1842480 2.0895433 10.739977 110-119
TACAG 2697940 2.073084 14.202243 160-169
ATCTC 2817065 2.066097 8.911668 100-109
AACCA 2025270 2.0549862 15.716501 70-79
GAATC 2669840 2.0514922 14.713701 190-199
GATAT 2524265 2.0505996 8.671249 180-189
GCTGT 4360735 2.040589 10.384391 140-149
GTCTC 3881280 2.039042 12.127289 180-189
ACACA 2008590 2.0380614 13.646501 110-119
GTTCA 3117930 2.0368779 15.288684 140-149
GTCCT 3859955 2.0278387 14.399078 130-139
AATAT 1784170 2.0234144 13.305669 110-119
CTCTG 3845065 2.0200162 14.825797 180-189
TGAAT 2469615 2.0062044 14.375078 190-199
ACACG 2759420 2.0055854 13.650629 120-129
CAGGC 3848880 2.003803 15.515326 140-149
TTGCA 3024845 1.9760673 14.5705595 150-159
ATGTA 2426185 1.9709238 7.289459 150-159
AATCG 2561565 1.9682943 13.484823 190-199
ACGGC 3774690 1.9651781 14.6094265 120-129
GGGGC 5859975 1.9465178 48.28501 15-19
CAGCC 3298925 1.9281836 10.499612 120-129
TGGAA 2812990 1.925296 9.670167 170-179
ATATG 2368295 1.9238966 8.899438 180-189
CCTTC 3252000 1.9180374 14.50872 200-209
TACTA 2099785 1.9150321 9.921514 240-247
TTTGC 3431100 1.9056622 12.496819 150-159
GGGCG 5728060 1.9026995 61.079834 20-24
GAGTC 3440490 1.8936611 12.719148 210-219
CATAG 2444560 1.8783882 8.644779 210-219
TCGGC 4220420 1.8680574 7.8914604 130-139
AGAAC 2010305 1.8169069 12.249482 160-169
AGTAT 2218935 1.8025632 12.546044 220-229
CTGTT 3224105 1.7906952 12.989462 140-149
AAAAA 1138820 1.7867994 8.040058 8
GACGT 3239065 1.7827958 10.39147 170-179
GTCAC 2850070 1.7611356 9.147424 140-149
GTTGT 3554340 1.7583939 7.4583864 210-219
CTCCA 2505350 1.738045 7.9962387 150-159
GCAGA 2678485 1.7340325 14.696256 150-159
ATTGT 2509335 1.7330797 7.8871417 170-179
CGTAG 3122735 1.718767 9.764889 210-219
TGGTT 3473335 1.7183193 18.572826 10-14
GGTCA 3113340 1.7135963 7.9912877 130-139
TGCAG 3107140 1.7101835 13.760801 150-159
AACTG 2222165 1.7075009 11.540613 160-169
CCAGA 2343010 1.7029325 24.189215 60-69
AGTCT 2604775 1.7016443 13.634195 210-219
TTCAG 2586645 1.6898005 9.943028 150-159
ATAGT 2079720 1.6894714 7.8317094 220-229
TGACT 2563340 1.6745757 12.33373 190-199
GAGAA 2080010 1.6744833 11.279117 160-169
CAAAG 1826900 1.6511459 30.804379 70-79
CACCA 2016080 1.6450775 13.889434 230-239
GTCTG 3513865 1.6442995 9.39111 210-219
CAAAA 1297740 1.6374236 21.042671 70-79
TCAGA 2118685 1.6279873 10.132394 130-139
TCCCA 2339745 1.6231593 11.0148535 240-247
ACTGA 2100830 1.6142677 9.418154 180-189
CTGGT 3434330 1.6070814 7.4675097 160-169
TTGTA 2303710 1.591064 9.680009 210-219
GCTCC 3190815 1.5855922 6.29376 150-159
TCTGG 3385340 1.5841568 12.15863 180-189
AATAG 1654355 1.580741 10.156005 240-247
GAACC 2174320 1.5803264 13.06416 70-79
TAGTT 2287380 1.5797858 10.656819 220-229
CCTTG 3000275 1.5762033 20.099052 20-24
TGCTC 2989055 1.5703089 5.173418 150-159
GTGCT 3352350 1.5687193 8.724658 220-229
GCTCA 2533675 1.5656266 11.521894 140-149
TGTGC 3345490 1.5655092 8.414223 220-229
ATAGA 1630005 1.5574745 10.798373 240-247
GGACG 3351680 1.554275 7.7467527 170-179
GGCCG 4167470 1.554141 13.314504 120-129
CTGTG 3294560 1.5416766 5.3557525 210-219
TTGGT 3107620 1.5373938 21.930237 25-29
GTCCC 3050800 1.5160154 18.237394 50-59
ACCAT 1737190 1.4986053 11.173148 45-49
GCTCT 2835525 1.4896514 10.1544 130-139
CCCAC 2259370 1.4825816 10.717603 240-247
CCACC 2219480 1.4564061 10.004003 230-239
AATTG 1787275 1.451902 9.869794 170-179
CCTCG 2911065 1.4465778 9.620392 130-139
TATAC 1584545 1.4451263 6.8223825 110-119
TGATA 1776850 1.4434332 8.176368 180-189
CGACT 2324800 1.436557 9.071659 190-199
TGGAC 2600560 1.4313598 11.006534 170-179
CCATC 2050670 1.4226183 11.104161 240-247
TCGAC 2278955 1.4082282 9.862125 190-199
CTCTT 2255750 1.4065617 8.817641 200-209
CACTG 2255735 1.3938799 7.824949 230-239
GGGAG 3369430 1.3917667 6.7525043 150-159
CATGT 2106550 1.3761644 6.882803 150-159
CTGCT 2605205 1.3686521 6.1025114 90-99
GAGGG 3306640 1.3658308 8.571921 200-209
CAGAC 1869355 1.3586736 13.794059 60-69
GCTGA 2464850 1.3566643 18.467834 30-34
CTGAT 2065620 1.3494257 7.827844 180-189
AGATC 1751975 1.3462092 7.212463 130-139
CCTCA 1940480 1.3461758 7.4337573 130-139
GAATT 1653880 1.343538 10.89031 170-179
CTTGA 2052375 1.340773 9.033543 200-209
ACGGA 2049345 1.3267317 9.516187 200-209
CTCGA 2143775 1.3246968 8.871239 190-199
GCGTA 2406270 1.3244216 10.39058 210-219
AAAGT 1373320 1.3122112 22.950102 70-79
GGAAT 1909305 1.3067865 9.091203 170-179
ACGTG 2368600 1.3036879 9.837523 170-179
ACTTC 1771785 1.2994659 6.8792953 220-229
ACTAT 1420840 1.2958254 6.7636275 230-239
TATGG 2218315 1.2908224 6.3797393 190-199
AGCCG 2472180 1.2870657 9.290278 120-129
ATCGG 2338085 1.2868924 7.9598565 190-199
AAGTA 1344590 1.2847595 16.633268 70-79
ATAAC 1192010 1.2786958 8.160306 80-89
TCCAC 1831285 1.2704235 9.984955 240-247
GTCCA 2044730 1.2634941 15.531619 70-79
GTGTT 2527790 1.2505418 7.7017426 160-169
CCATG 2017235 1.2465043 6.8027306 70-79
GGAGT 2537005 1.2437925 7.2645507 210-219
ACTCG 2007260 1.2403405 9.087394 190-199
GGCTC 2790715 1.2352363 8.31705 130-139
GTGGA 2510825 1.2309574 18.682907 25-29
ACTGG 2233470 1.2293118 7.670721 160-169
GATCT 1875505 1.2252277 5.57996 130-139
AGCTT 1873995 1.2242413 6.7090163 150-159
AGAGC 1879295 1.2166425 7.6756396 140-149
TTGAG 2075375 1.2076466 8.66641 200-209
ACCGT 1934575 1.1954265 16.107313 45-49
TTCCA 1616715 1.1857343 8.36366 230-239
TATCA 1286380 1.1731958 6.802476 70-79
ATAAA 877355 1.1703346 5.441908 7
GCATA 1518490 1.1668004 8.441517 210-219
GGAGG 2790445 1.1526128 14.194792 30-34
TTGGC 2457865 1.1501485 7.584163 60-69
ATATC 1256990 1.1463917 7.2853794 70-79
TCTAC 1554180 1.1398696 7.8929996 180-189
TCACG 1842895 1.1387749 9.785228 200-209
ATACC 1315300 1.1346575 6.7761917 70-79
GTGGT 2721885 1.1345152 9.342514 50-59
GTCTA 1734880 1.1333603 7.9780955 180-189
ACTGT 1732085 1.1315345 5.871563 230-239
TGGTC 2415705 1.1304199 10.655394 50-59
AGGTT 1933450 1.1250614 23.368956 10-14
GCTTC 2121880 1.114736 6.010199 150-159
AGTTC 1695375 1.1075525 5.3985205 160-169
TGCCC 2219115 1.1027312 5.8703227 60-69
GAGCT 2002915 1.1024133 6.7718835 140-149
GAAGA 1366110 1.0997679 17.522572 35-39
CCATA 1273600 1.0986844 9.408625 70-79
TACTG 1676750 1.0953852 7.0087757 180-189
CAATA 1016565 1.090492 6.795878 100-109
AAAAG 963670 1.0830448 13.862625 70-79
CTGAA 1409105 1.0827495 13.997967 35-39
TCTGT 1941785 1.0784838 6.6662426 210-219
AGTTG 1850175 1.0766042 5.1002393 220-229
ATCCA 1247765 1.0763977 16.17245 240-247
CGGCT 2430505 1.0757989 7.82769 130-139
CTCGG 2423920 1.0728841 8.39918 130-139
AGGGG 2547300 1.05218 5.037812 60-69
GGGGT 2963150 1.0405852 7.8243704 10-14
TATGT 1506310 1.0403374 7.703931 220-229
CTACC 1497830 1.0390947 9.221724 230-239
GTCCG 2345260 1.0380673 5.239554 130-139
CTGCG 2339195 1.0353829 6.9359307 210-219
GCGGT 2620650 1.0332092 5.9714284 130-139
ATACG 1324130 1.0174551 5.251022 120-129
TAGAT 1248390 1.014136 8.301482 240-247
CTGCC 2036290 1.0118811 5.8148975 200-209
CATGG 1834050 1.0094693 5.286982 190-199
CGAAC 1385825 1.0072372 13.837256 70-79
CAGCG 1923820 1.0015787 6.576922 160-169
TATAA 881920 1.0001792 6.904245 240-247
AAAAT 748455 0.9983903 5.3472633 8
AGAGA 1231620 0.99149865 12.417666 35-39
CTTCT 1589670 0.9912308 5.539129 210-219
AGAAA 878575 0.98740864 5.1479664 6
AACTT 1081950 0.98675305 6.006883 200-209
TGCTA 1506585 0.98421997 5.258107 220-229
ATGGG 1976595 0.9690458 27.059563 15-19
AGCTG 1755580 0.96627903 5.2437143 90-99
TGCGT 2051235 0.9598675 8.317801 210-219
ACCCA 1160705 0.94711006 13.455774 240-247
AAGAG 1170835 0.9425644 13.051292 35-39
CGGAG 2029055 0.9409338 6.8046603 210-219
ACAAT 874550 0.93814933 7.8645597 100-109
TGTAA 1142210 0.9278801 7.3648033 230-239
TCGAA 1206205 0.92684203 17.343437 70-79
TCCCC 1655715 0.92369974 5.0538445 80-89
TAGGC 1672955 0.9208019 5.692275 160-169
AGTTT 1311565 0.9058363 5.1219807 220-229
CCATT 1230655 0.9025893 5.563318 50-59
GAGTT 1545725 0.89944685 5.0955544 160-169
ACTAA 833820 0.89445734 13.115771 240-247
CCCAA 1082725 0.8834801 10.459591 240-247
TTCTG 1587425 0.88166934 5.6016574 210-219
TGAAC 1141110 0.87682337 6.6078706 190-199
TGCAT 1334445 0.87176454 6.4038243 210-219
CTGTA 1333960 0.8714477 5.184983 230-239
AGCGT 1576465 0.86769336 6.771948 160-169
CGTGG 2198545 0.86679137 6.412583 50-59
TGAAG 1263910 0.8650584 9.154307 35-39
CGGTC 1953820 0.8648068 5.8496103 130-139
CCAGC 1477540 0.8636051 5.86652 80-89
CATCA 995900 0.8591237 5.929274 230-239
ACGTC 1373165 0.8485159 10.022466 70-79
CCAAT 971505 0.83807904 11.273562 240-247
CTTCC 1406925 0.8298077 5.8363404 230-239
GTATG 1424570 0.8289476 5.400372 220-229
ATCAA 767445 0.8232554 12.505633 70-79
TAACC 948685 0.8183932 6.657414 80-89
CGTCC 1619750 0.8048925 8.828271 70-79
GGTGG 2253215 0.7912735 18.639599 25-29
GAGGC 1677645 0.77797455 12.096763 30-34
AAAGG 957515 0.7708341 8.907633 10-14
AACCG 1046505 0.7606146 5.7231126 190-199
AGCCA 1038155 0.75454557 5.909456 80-89
TGAGA 1093665 0.7485376 5.70919 240-247
GGGCC 1998155 0.7451559 14.11609 15-19
GGTGC 1889715 0.7450332 5.185878 220-229
GTCGA 1349895 0.74298817 12.528178 70-79
AAAGA 652595 0.73343515 6.2183995 8
ATGAT 899450 0.7306728 7.647491 240-247
GACCG 1397945 0.7277978 14.898151 45-49
CTAAT 790180 0.7206548 10.066854 240-247
AAGGT 1049680 0.7184329 10.127382 10-14
AATCC 825900 0.71247137 9.874566 240-247
GTAGC 1293345 0.71186286 5.500395 80-89
GGTCC 1568715 0.69435036 10.818653 50-59
TGGGC 1757050 0.692729 15.355595 15-19
GGAAG 1172110 0.6758979 5.9828153 30-34
AGACC 920835 0.6692759 5.0546637 240-247
CATAC 774700 0.66830313 8.008406 70-79
AAAGC 729095 0.6589535 9.392103 70-79
CATTG 994505 0.649689 5.0507593 50-59
TAGGT 1102585 0.6415866 5.482982 10-14
TATGA 788595 0.64061916 6.1382794 240-247
CAATC 716315 0.61793673 9.6377945 240-247
ATAGG 902215 0.61750346 5.3935714 10-14
GAGGT 1220315 0.59827185 6.604737 10-14
GGGTC 1510790 0.59563935 6.109773 70-79
AGAAG 728690 0.5866218 5.490218 9
AATGG 852430 0.5834291 6.8238196 10-14
CGATG 1030685 0.5672936 12.319497 15-19
GAAAA 503600 0.56598353 6.0029054 8
AAATG 590315 0.56404763 5.3118033 9
CATCC 776045 0.53836834 7.1893315 240-247
TAAAA 403025 0.53760916 5.908366 8
AAGGG 912700 0.5263089 6.563557 10-14
AAGAA 468060 0.526041 5.5678725 8
GAAGG 903020 0.520727 5.6125603 10-14
GGTCG 1283190 0.5059065 6.1847997 70-79
AAGCA 558715 0.5049647 6.292769 70-79
TGATC 758405 0.4954499 5.8058553 240-247
ATAAG 498890 0.47669083 6.267905 9
GATCC 757490 0.46807364 5.7914658 240-247
CGGTT 961555 0.44995594 8.04788 10-14
TAAGG 652835 0.44682017 6.182923 10-14
ACGAT 562210 0.43199944 5.570163 10-14
TAAAG 419860 0.40117744 5.2007356 9
AAGCT 511810 0.3932723 7.6734767 30-34