FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_10_SL248339.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_10_SL248339.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7811412
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 8676595 8.668397 61.120476 110-119
CACAG 11718625 7.5022593 41.54843 110-119
ATACA 7940930 7.4876647 38.09751 110-119
TCCCT 13908265 7.314847 108.16073 30-34
CAGTA 10034010 6.806195 39.92398 60-69
AGTAA 7578055 6.3660984 47.4254 110-119
GGAGA 12194950 6.1969295 91.02504 35-39
CCCTG 13982665 6.183699 70.1337 30-34
GTAAT 8228985 5.9141264 39.681614 110-119
GGTGA 13479140 5.859863 99.25139 45-49
AATAC 5795645 5.464832 39.9639 110-119
GTAGT 10030755 5.1860037 24.978714 80-89
ACAGT 7609735 5.1617785 35.53689 110-119
AGACG 9022915 5.1463914 98.2928 40-44
GTGAC 10332490 5.041847 82.02431 45-49
ACTCC 8167685 5.021143 123.92739 30-34
GAGAC 8546290 4.87454 137.48569 40-44
ACCAG 7478930 4.788008 93.86352 45-49
CCCCA 8231245 4.775893 99.24605 60-69
CTCAG 8462705 4.635039 35.040325 140-149
CACTC 7388245 4.541976 100.2481 25-29
GCACA 7059650 4.5195847 33.65134 110-119
GTGTC 10469180 4.3704495 23.893522 120-129
CGTGT 10455075 4.3645616 23.336275 120-129
GGCCC 11696785 4.3496165 58.542896 60-69
TGACC 7871330 4.3111415 139.97568 45-49
CTCCC 8621245 4.279449 70.14758 30-34
TAGTA 5933760 4.2645607 31.43603 80-89
ATGCA 6222185 4.220586 97.23799 25-29
GACCA 6457130 4.1338515 105.308685 45-49
AGTAG 6833290 4.1295266 28.52902 70-79
CCCAG 7798755 4.0313907 85.00021 60-69
TGAGG 8884645 3.8624725 67.27424 35-39
CAGGG 9323205 3.825393 36.123806 50-59
AGGAG 7385505 3.7529848 91.362595 35-39
CCACT 6087995 3.742638 29.652618 240-247
TACAC 4884610 3.7189403 27.790108 110-119
TGCAC 6776555 3.7115307 117.674 25-29
ACGGT 7563905 3.6908875 93.57992 40-44
ATGGT 7094640 3.668002 26.33541 190-199
CTGAG 7497690 3.658577 103.50255 35-39
GACGG 8902650 3.6528354 104.545845 40-44
GGGTT 9748790 3.625807 43.379086 50-59
TGTCC 7582065 3.552715 20.581621 120-129
AGATA 4196625 3.5254598 19.124386 150-159
GAGGA 6903780 3.508193 120.154594 35-39
CCTGA 6398660 3.5045576 84.44098 30-34
GCACT 6270370 3.4342923 90.73027 25-29
CCAGG 7450715 3.4313748 41.13267 50-59
GCCCC 8214605 3.4287088 71.42974 60-69
CCCTT 6492825 3.4148054 31.704727 20-24
CTTCA 5224485 3.4029968 17.155447 200-209
GGATG 7810240 3.3953896 70.546234 20-24
TCTTG 6818265 3.38502 18.68608 170-179
TGGTG 9021170 3.3551881 18.379484 190-199
CTCTC 6230830 3.2770138 13.824533 100-109
CCTGG 8284330 3.2640467 29.86411 50-59
TCAGG 6627400 3.2339098 21.100029 140-149
CCGTG 8198400 3.23019 23.501007 120-129
CTTGG 7702035 3.215281 17.722795 20-24
CAGAT 4723190 3.2037988 17.619774 150-159
TTCCC 6087950 3.2018673 51.351826 50-59
CGGAT 6506485 3.1749082 80.48534 20-24
TCGCA 5785285 3.168611 24.143627 100-109
TGTCT 6372680 3.1638029 20.18702 180-189
AGGCT 6479645 3.161811 21.100021 30-34
GGTTG 8487700 3.156778 75.426056 15-19
GGTTC 7414265 3.0951488 49.102875 50-59
CGCAC 5949565 3.0754936 24.722559 100-109
GATGC 6294780 3.0716045 98.21594 25-29
TCTCA 4710665 3.0683174 21.59208 130-139
AGGGT 6960805 3.0261104 50.91371 50-59
ACAGA 3787165 3.0027237 15.093837 140-149
AGTCA 4390485 2.978121 33.11357 70-79
TGGCC 7531225 2.9673214 43.51667 60-69
CCAGT 5408580 2.9622884 38.250813 60-69
GTTGG 7942435 2.953981 53.755188 15-19
TCAAA 3050280 2.876171 58.3134 70-79
GACTC 5202675 2.8495145 20.76344 190-199
ACCAC 3941225 2.8320835 20.332169 230-239
TCTCT 5047400 2.8126423 15.295664 180-189
GCCGT 7101910 2.7981703 22.239176 120-129
TTCTT 4735475 2.7959201 21.303617 170-179
GTTCC 5935240 2.781065 55.75392 50-59
CGGTG 7875990 2.7646787 66.04637 40-44
GCCCT 6150040 2.7197962 17.191736 20-24
CGGCC 7172335 2.6671352 12.511633 120-129
TCACA 3493880 2.660096 12.171565 140-149
GATGG 6066860 2.6374807 17.565613 15-19
GCGGA 6407485 2.6290472 88.05795 20-24
AGGGA 5114505 2.5989633 9.744577 150-159
AGATG 4266595 2.5784092 17.08385 180-189
GGCGG 8541205 2.5210693 42.50253 20-24
AAAAA 1842755 2.515357 13.950765 120-129
TTGGG 6668585 2.4802058 81.01448 15-19
TGTTC 4952710 2.45884 13.011963 140-149
CTGGC 6224955 2.4526482 35.82088 60-69
TCCAT 3754595 2.4455757 15.253244 70-79
TCCTC 4634480 2.4374368 16.805277 130-139
ACAGC 3797330 2.4310489 11.230053 120-129
GTTCT 4841045 2.403402 18.62824 160-169
GGCTG 6840820 2.4013069 11.719773 140-149
ACTCT 3672100 2.3918421 13.937865 200-209
CACTA 3140775 2.3912563 15.92259 240-247
CAGCT 4361550 2.3888283 12.471746 150-159
TGGAG 5446405 2.3677464 14.412752 25-29
GAGAT 3907725 2.3615353 16.638733 180-189
TCAGC 4276895 2.342463 15.197042 140-149
TACCA 3053025 2.3244472 16.766659 230-239
CACGG 4933360 2.2720244 9.428095 120-129
TCATT 3250170 2.2430482 16.604963 150-159
CTGGA 4596190 2.2427595 14.504965 180-189
TGTAG 4337300 2.2424288 8.881685 210-219
CATTT 3245305 2.2396905 17.726824 150-159
AATAA 1917005 2.2386386 9.320639 110-119
CAGAG 3899975 2.224425 10.134817 140-149
CTACT 3407720 2.219637 8.02393 180-189
TTCAT 3194100 2.2043524 16.152319 140-149
GAACT 3228775 2.1901186 11.728081 160-169
TGGGG 6980210 2.182974 52.997776 15-19
TTCAC 3342280 2.1770122 18.77046 200-209
TAGTC 3714475 2.1555398 27.480938 70-79
TCTCG 4592190 2.1517546 17.459288 100-109
GTGAA 3542365 2.140739 17.916645 190-199
TCTGC 4560785 2.1370392 12.4177065 210-219
ACTAC 2799085 2.1311076 12.530262 230-239
GATAC 3134785 2.1263642 14.475805 150-159
ATCTC 3257560 2.1218293 9.407411 100-109
TTGGA 4100340 2.1199179 16.619865 170-179
GATAT 2942250 2.114579 8.92701 180-189
TTGTC 4257985 2.1139343 13.376808 170-179
CTCGC 4748385 2.0999277 16.475252 100-109
ATTTG 3408705 2.095861 15.625762 150-159
GTCAA 3079715 2.0890093 41.729557 70-79
AGCTC 3781380 2.0710685 13.050902 140-149
ACACG 3229260 2.067371 13.192901 110-119
GTCTC 4355845 2.0410106 11.900983 180-189
ATATA 2024285 2.0223725 10.022064 110-119
GTTCA 3481870 2.0205572 15.114309 140-149
ACACA 2258195 2.0096574 13.580606 110-119
TACAG 2962385 2.0094228 13.848411 160-169
GCTGT 4809650 2.00783 10.121285 140-149
GAATC 2957420 2.006055 14.363905 190-199
GTCCT 4262475 1.9972605 14.208349 130-139
CTCTG 4245080 1.9891099 14.61594 180-189
CAGGC 4266200 1.9647688 15.190853 140-149
TGAAT 2725615 1.9588846 13.978049 190-199
ATGTA 2725340 1.958687 7.1212797 150-159
AACCA 2200380 1.9582055 15.306199 70-79
GGGGC 6590615 1.9453224 48.084362 15-19
AATAT 1942085 1.9402502 12.496144 110-119
ACGGC 4207305 1.9376451 14.169925 120-129
TTGCA 3332350 1.9337894 14.339268 150-159
CCTTC 3659260 1.9245337 14.150353 200-209
CAGCC 3694810 1.9099488 10.281675 120-129
ATATG 2649145 1.903926 9.14655 180-189
AATCG 2796455 1.8968704 13.119329 190-199
GGGCG 6414240 1.8932626 59.865475 20-24
TGGAA 3130870 1.8920623 9.391547 170-179
GAGTC 3850975 1.8791239 12.376435 210-219
TTTGC 3763190 1.8682866 12.201106 150-159
CTCCA 3038420 1.8678905 8.168378 150-159
TACTA 2284465 1.8428427 9.817861 230-239
GTCAC 3364100 1.8425232 9.3204 130-139
TCGGC 4669740 1.8398894 7.797048 130-139
CATAG 2710890 1.8388306 8.5002985 210-219
AGAAC 2269720 1.7995894 11.986873 160-169
TTCAG 3075255 1.7845953 10.020599 150-159
GTTGT 4003010 1.770576 7.699224 210-219
GACGT 3615480 1.7642117 10.486736 170-179
CTGTT 3552950 1.7639102 12.792819 140-149
CCAGA 2748900 1.7598445 23.75912 60-69
AGTAT 2446385 1.7582035 12.152459 220-229
GGTCA 3512220 1.7138247 8.280709 130-139
TGGTT 3868565 1.7111094 18.240562 10-14
AACTG 2514635 1.7057086 11.5229845 160-169
CGTAG 3491290 1.7036119 9.6769705 210-219
ATTGT 2763180 1.6989564 7.629008 170-179
GTCTG 4051920 1.6915089 9.240802 210-219
TGCAG 3458100 1.6874163 13.539975 150-159
GCAGA 2957470 1.6868495 14.338131 150-159
AGTCT 2906185 1.6864825 13.280619 210-219
CACCA 2338570 1.6804484 13.829435 230-239
TGACT 2874045 1.6678315 12.233202 190-199
GAGAA 2353955 1.6627996 10.92104 160-169
ATAGT 2277240 1.63664 7.667583 220-229
ACTGA 2411500 1.6357507 9.52949 180-189
TCCCA 2654830 1.6320757 10.88747 240-247
CAAAG 2038055 1.6159095 29.574446 70-79
CTGGT 3861775 1.6121314 7.588512 160-169
TTGTA 2606775 1.6027901 10.044078 210-219
TCAGA 2355735 1.5979246 10.037759 130-139
TAGTT 2597870 1.5973147 10.597691 220-229
CCTTG 3354455 1.5717912 20.287046 20-24
GCTCC 3552730 1.5711607 6.2417445 150-159
GTGCT 3755880 1.5679245 8.895339 220-229
GCTCA 2862085 1.567569 11.649718 140-149
AATAG 1862345 1.5645006 9.778374 240-247
TCTGG 3736095 1.5596652 11.940616 180-189
GAACC 2433560 1.557964 12.540532 70-79
ATAGA 1854020 1.557507 10.479966 240-247
TGTGC 3730585 1.5573648 8.5200615 220-229
TTGGT 3518935 1.5564642 21.927069 25-29
GGACG 3786595 1.553673 7.794411 170-179
CAAAA 1409070 1.5530264 20.069855 70-79
TGCTC 3309665 1.550804 5.142249 150-159
CTGTG 3684650 1.5381889 5.3969846 210-219
GGCCG 4638040 1.5365955 13.036396 120-129
GTCCC 3366115 1.488632 17.739109 50-59
CCCAC 2555615 1.4828066 10.640779 240-247
CCACC 2553655 1.4816693 10.187767 230-239
GCTCT 3155875 1.478743 10.03595 130-139
ACCAT 1910015 1.4542065 10.60584 45-49
CGACT 2628660 1.4397216 9.493806 190-199
TGATA 1997905 1.4358834 8.3974695 180-189
AGATC 2109400 1.4308326 7.3455434 130-139
CCTCG 3220425 1.424202 9.470798 130-139
CTCTT 2552880 1.4225816 8.755764 200-209
CTGCT 3033700 1.4214957 5.920273 90-99
TATAC 1760270 1.4199827 6.5403824 110-119
AGCTT 2445280 1.4190156 6.7446914 150-159
TGGAC 2906395 1.418206 11.033811 170-179
CTTGA 2437805 1.4146779 9.073324 200-209
TCGAC 2573785 1.4096667 10.228221 190-199
CACTG 2556400 1.4001448 7.8588877 230-239
AATTG 1946710 1.3990898 9.37236 170-179
GGGAG 3799250 1.3888305 7.0172586 150-159
GAGGG 3796040 1.3876573 8.926035 200-209
AAATA 1181005 1.3791533 5.309049 9
CATGT 2354875 1.366553 6.729633 150-159
CAGAC 2130530 1.3639643 13.513869 60-69
GGAAT 2251435 1.360598 8.899535 170-179
CTGAT 2329670 1.3519264 7.922349 180-189
GCTGA 2751430 1.3425894 18.103924 30-34
ATCGG 2735205 1.3346722 7.884371 190-199
CCATC 2150815 1.3222288 11.217928 240-247
GCGTA 2699005 1.3170079 10.117852 210-219
CTCGA 2404195 1.3167819 9.1422 190-199
CCTCA 2122885 1.3050586 7.261193 130-139
GAATT 1812930 1.3029429 10.453513 170-179
AGAGC 2277970 1.2992836 7.8731537 140-149
TATGG 2508270 1.2968013 6.526367 180-189
TCCAC 2108545 1.2962431 10.600302 240-247
ACGTG 2654290 1.2951889 10.025062 170-179
AAAGT 1531450 1.2865255 22.102896 70-79
ACGGA 2247695 1.2820158 9.250646 200-209
ACTTC 1954595 1.273136 6.6282797 220-229
AGCCG 2742410 1.2629977 8.997645 120-129
ACTAT 1561600 1.2597187 6.678915 230-239
ACTCG 2272960 1.2449043 9.276589 190-199
AAGTA 1479705 1.2430562 15.949469 70-79
GTGTT 2807140 1.2416294 7.5848594 160-169
ACTGG 2537660 1.238278 7.722321 160-169
CCATG 2260175 1.2379019 6.7387414 70-79
GGCTC 3121260 1.2297843 8.287799 130-139
GGAGT 2814285 1.22347 7.188684 210-219
GATCT 2094220 1.2152927 5.4283967 130-139
GTGGA 2792905 1.2141753 18.76297 25-29
TTGAG 2346800 1.2133198 8.70858 200-209
ATAAC 1282170 1.2089843 7.6576796 80-89
GAAGA 1695715 1.1978283 17.385296 35-39
ATATC 1478010 1.1922879 6.9330387 70-79
TTCCA 1808115 1.1777254 8.1611185 230-239
CTGAA 1723730 1.1692277 13.446489 35-39
ACCGT 2132895 1.1681902 15.879264 45-49
GCATA 1716435 1.1642793 8.27849 210-219
GGAGG 3181620 1.1630535 13.87274 30-34
GTCCA 2103125 1.1518853 14.275948 70-79
ATCCA 1499040 1.1413071 16.561289 240-247
TCTAC 1751100 1.1405885 7.7536345 180-189
ATAAA 971635 1.1346551 5.647716 7
TTGGC 2714180 1.1330578 7.285495 60-69
GCTTC 2417965 1.1329818 5.950366 150-159
GTCTA 1951850 1.1326742 7.789853 180-189
ACTGT 1950495 1.131888 5.9647217 230-239
TGGTC 2699055 1.1267438 10.457262 50-59
TATCA 1395010 1.125333 6.463922 70-79
AGGTT 2172060 1.1229774 23.106468 10-14
GTGGT 3017025 1.1221035 9.177376 50-59
ATACC 1470205 1.1193533 6.572091 240-247
TACTG 1923160 1.1160252 7.077788 180-189
GAGCT 2259970 1.1027763 6.8654337 140-149
AGTTC 1898335 1.1016191 5.4005985 160-169
TCACG 2005710 1.098531 9.409607 200-209
AGTTG 2119935 1.0960282 5.1769137 220-229
TGCCC 2465570 1.0903746 5.8190756 60-69
CAATA 1143475 1.078206 6.3703804 100-109
TCTGT 2170135 1.0773929 6.56563 210-219
CGGCT 2716520 1.0703156 7.710261 130-139
CTTCT 1918985 1.0693463 5.7672825 210-219
CTCGG 2689280 1.0595832 8.338466 130-139
CCATA 1385550 1.0549005 8.993909 70-79
TAGAT 1459735 1.0491036 8.310692 240-247
AAAAG 1061630 1.0424616 13.079625 70-79
TATGT 1694705 1.0419986 7.742827 220-229
AAGAG 1473595 1.040926 12.923309 35-39
GCGGT 2960040 1.0390515 6.1321716 130-139
GGGGT 3311940 1.0357682 7.7713704 10-14
GTCCG 2625665 1.0345186 5.12841 120-129
CTGCG 2622205 1.0331554 6.8786244 210-219
CTACC 1668270 1.0255809 8.889997 230-239
CATGG 2081280 1.0155826 5.389226 190-199
CTGCC 2290050 1.0127527 5.89209 200-209
AGAGA 1424750 1.0064226 11.796659 35-39
ACCCA 1396210 1.0032878 13.762935 240-247
ATACG 1478230 1.0027019 5.059059 120-129
CAGCG 2163685 0.99647003 6.379037 160-169
TGAAC 1451070 0.98427904 6.540603 190-199
AACTT 1208190 0.9746283 5.91736 200-209
ATGGG 2239030 0.9733863 27.296276 15-19
TGCTA 1674875 0.9719434 5.1240273 220-229
AGCTG 1989555 0.97082436 5.1429086 80-89
CGAAC 1514865 0.9698159 13.236781 70-79
TATAA 960355 0.9594476 6.7083454 240-247
TGCGT 2276250 0.9502402 8.173015 210-219
TTCTG 1890795 0.93871063 5.796715 210-219
TGTAA 1277690 0.9182688 7.2221355 230-239
ACGTC 1667820 0.913468 9.665223 70-79
CGGAG 2224705 0.912816 6.6835637 210-219
CCCAA 1269280 0.91207856 10.937874 240-247
TAGGC 1866860 0.91095406 5.717863 160-169
CCAGC 1758815 0.9091798 5.884727 80-89
TCCAG 1654580 0.90621644 5.336227 240-247
GTATG 1752225 0.90591836 5.3984213 220-229
TCGAA 1332255 0.90368533 16.696257 70-79
ACAAT 955835 0.9012763 7.274045 100-109
GAGTT 1730935 0.89491117 5.10312 160-169
CCATT 1363295 0.8879896 5.424742 50-59
ACTAA 933970 0.88065934 12.573878 240-247
TGCAT 1507085 0.87457347 6.303591 210-219
CCAAT 1148155 0.8741578 11.633478 240-247
CGGTC 2212870 0.8718763 5.988267 130-139
AGCGT 1766615 0.86203843 6.5362115 160-169
TGAAG 1414030 0.85453343 8.86278 35-39
CGTGG 2418025 0.84879017 6.312917 50-59
CTTCC 1612625 0.8481365 5.7384105 230-239
CATCA 1101195 0.83840436 5.8125114 230-239
GAGGC 1940600 0.79624534 11.840235 30-34
ATCAA 843085 0.79496205 11.8318205 70-79
GGTGG 2519150 0.7878329 18.84413 25-29
GACCG 1708325 0.7867572 14.683263 45-49
TAACC 1025275 0.78060204 6.1664686 80-89
AGACC 1212530 0.77626115 5.2098055 240-247
AAAGG 1077055 0.7608159 8.765883 10-14
AACCG 1183315 0.7575578 5.578549 190-199
AATCC 994010 0.75679815 10.470285 240-247
GGAAG 1486760 0.755505 6.295413 30-34
GGTGC 2151650 0.7552855 5.339694 220-229
TGAGA 1247400 0.7538348 5.705174 240-247
GGGCC 2267570 0.7512522 14.176625 15-19
AAAGA 758415 0.74472123 6.040624 8
AGCCA 1160745 0.74310845 5.8588047 80-89
AAGGT 1214540 0.73397666 10.152739 10-14
GTCGA 1502140 0.732985 12.053428 70-79
ATGAT 1011790 0.72716796 7.405235 240-247
CTAAT 880110 0.7099712 9.6665325 240-247
CGTCC 1592730 0.70436954 7.9378567 70-79
TGGGC 1990725 0.69879663 15.4765625 15-19
GTAGC 1425415 0.6955463 5.3554025 80-89
GGTCC 1717470 0.6766875 10.585458 50-59
CATAC 862800 0.6569003 7.711564 70-79
CAATC 852030 0.6487004 10.153393 240-247
GAAAA 658595 0.6467036 5.3946686 8
TATGA 896105 0.64402574 6.1397896 240-247
AAAGC 809220 0.641605 8.930009 70-79
TAGGT 1212225 0.62673277 5.276148 10-14
AGGAT 1012655 0.6119726 5.182882 10-14
GAGGT 1401075 0.60909736 6.709913 10-14
GGGTC 1718685 0.60330343 5.8380466 70-79
AGAAG 843765 0.59602326 5.344517 9
AATGG 942185 0.56938577 6.5169153 10-14
CGATG 1153340 0.56278443 12.089635 15-19
CATCC 910955 0.5600161 7.5759063 240-247
GAAGG 1063120 0.54023015 5.6085863 10-14
TAAAA 461125 0.5384922 5.8592615 6
AAGAA 528580 0.5190361 5.1627817 9
AAGGG 1017215 0.5169033 6.3311415 10-14
GGTCG 1424335 0.49997887 5.9283714 70-79
AAGCA 619085 0.49085295 5.9143386 70-79
TGATC 845705 0.49076942 5.742035 240-247
GATCC 870985 0.47704005 5.741044 240-247
ATAAG 549880 0.4619378 5.4509835 9
CGGTT 1088580 0.4544371 8.384811 10-14
TAAGG 721615 0.43608987 5.8717914 10-14
ACGAT 635210 0.4308709 5.5138135 10-14
TAAAG 501440 0.4212448 5.4339647 9
AAGCT 587070 0.39821693 7.834866 30-34
TGGGT 1035680 0.38519403 5.080673 10-14