FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_16_SL248345.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_16_SL248345.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6623420
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7569625 8.926852 62.51845 110-119
ATACA 6898670 7.6938148 38.301224 110-119
CACAG 10092715 7.6250057 43.002766 110-119
TCCCT 12024540 7.370753 109.69278 30-34
CAGTA 8675420 6.930591 40.75817 60-69
AGTAA 6582765 6.5405607 49.033096 110-119
GGAGA 10483950 6.286627 92.5463 35-39
CCCTG 12070465 6.233759 70.989456 30-34
GTAAT 7188600 6.072559 40.65507 110-119
GGTGA 11635695 5.9320645 103.07017 45-49
AATAC 5018345 5.596763 40.426174 110-119
AGACG 7831435 5.2711306 101.27945 40-44
ACAGT 6587805 5.262844 36.745422 110-119
GTAGT 8656875 5.2383204 25.421093 80-89
GTGAC 8921135 5.105089 84.4419 45-49
ACTCC 6833785 4.9270153 126.55071 30-34
GAGAC 7210355 4.8530984 141.93633 40-44
CCCCA 7078435 4.826277 101.67944 60-69
ACCAG 6380645 4.820552 95.66937 45-49
CTCAG 7341590 4.7156663 35.487347 140-149
CACTC 6276725 4.5253873 100.69545 25-29
GCACA 5977735 4.516155 35.201893 110-119
CGTGT 9081715 4.41847 23.494492 120-129
GGCCC 10144370 4.414015 59.73374 60-69
GTGTC 9064690 4.410187 24.039959 120-129
TGACC 6825980 4.3844786 143.10387 45-49
TAGTA 5179505 4.3753786 32.30915 80-89
CTCCC 7426305 4.304954 71.59211 30-34
ATGCA 5308175 4.2405777 99.259094 25-29
AGTAG 5924910 4.2168856 29.167656 70-79
GACCA 5561390 4.2016077 107.73415 45-49
CCCAG 6680365 4.057938 86.904175 60-69
TGAGG 7592420 3.8707378 69.00509 35-39
CAGGG 7974720 3.8448665 37.275845 50-59
TACAC 4222210 3.7860758 27.644802 110-119
AGGAG 6302500 3.7792497 93.06155 35-39
ACGGT 6566430 3.7576168 94.75893 40-44
CCACT 5187070 3.7397685 29.223125 240-247
TGCAC 5793855 3.7215216 118.6783 25-29
ATGGT 6132430 3.7107646 26.743559 190-199
GACGG 7681730 3.7036068 106.37073 40-44
CTGAG 6464225 3.6991303 106.60012 35-39
AGATA 3669020 3.6454968 19.723227 150-159
GGGTT 8334490 3.6125453 44.043003 50-59
TGTCC 6592165 3.599994 20.496973 120-129
GAGGA 5951085 3.5685263 122.849724 35-39
CCTGA 5532455 3.5536187 85.94946 30-34
GCCCC 7098425 3.4668927 72.87918 60-69
CCAGG 6402325 3.4647608 42.338173 50-59
GCACT 5371735 3.4503849 91.05351 25-29
CCCTT 5605855 3.4362538 31.521355 20-24
GGATG 6686695 3.4089847 70.672775 20-24
TCTTG 5875625 3.3929338 19.075888 170-179
TGGTG 7734025 3.3522768 18.550093 190-199
CTTCA 4368610 3.3305314 17.572575 200-209
CTCTC 5425205 3.3255196 13.958329 100-109
CCGTG 7137700 3.2840872 23.83029 120-129
CAGAT 4101625 3.2766929 18.129923 150-159
CCTGG 7104270 3.2687054 30.109907 50-59
TCAGG 5711985 3.2686636 21.136635 140-149
CTTGG 6673775 3.2469492 17.724724 20-24
TCGCA 5021020 3.2251124 24.53287 100-109
AGGCT 5626980 3.2200198 21.682993 30-34
TGTCT 5524275 3.1900437 20.667051 180-189
TTCCC 5201350 3.188302 51.84151 50-59
CGGAT 5555330 3.1790185 80.614746 20-24
GGTTG 7241310 3.1387117 76.48715 15-19
CGCAC 5154595 3.1311212 24.77433 100-109
TCTCA 4085590 3.114763 21.753017 130-139
GGTTC 6360415 3.0944927 49.86772 50-59
GATGC 5379060 3.0781484 100.169975 25-29
AGGGT 5965575 3.0413456 51.93422 50-59
ACAGA 3233160 3.0379891 15.304176 140-149
TGGCC 6525825 3.0025606 44.69564 60-69
TCAAA 2661790 2.9685893 60.474678 70-79
AGTCA 3675625 2.9363713 34.262695 70-79
GTTGG 6761390 2.9306927 54.33762 15-19
CCAGT 4513355 2.899028 38.203556 60-69
ACCAC 3403000 2.885784 20.765356 230-239
GACTC 4437765 2.8504748 20.317162 190-199
TCTCT 4397200 2.850147 15.465466 180-189
TTCTT 4112735 2.8188334 21.782911 170-179
CGGTG 6844680 2.8056908 66.62298 40-44
GTTCC 5123465 2.797934 56.626125 50-59
GCCGT 6071780 2.7936523 22.69399 120-129
GCCCT 5327185 2.7512105 17.211803 20-24
CGGCC 6229155 2.710428 12.937418 120-129
GATGG 5247070 2.6750402 17.705263 15-19
TCACA 2966705 2.6602583 12.518681 140-149
GCGGA 5460415 2.6326404 88.9443 20-24
AGATG 3693330 2.6286225 17.145983 180-189
TCCAT 3410230 2.5998836 16.771822 70-79
AGGGA 4288305 2.571452 9.384165 150-159
GGCGG 7294880 2.5193439 43.089985 20-24
TGTTC 4349490 2.5116532 13.109474 140-149
ACAGC 3310160 2.5008125 11.403758 120-129
TCCTC 4063740 2.4909744 17.145323 130-139
TTGGG 5712195 2.4759238 81.35391 15-19
CTGGC 5354340 2.4635549 36.681503 60-69
GGCTG 5955955 2.441395 11.919738 140-149
GTTCT 4201625 2.4262671 18.171633 160-169
ACTCT 3169550 2.4163945 14.224621 200-209
CAGCT 3743605 2.4046009 12.796647 150-159
GAGAT 3364750 2.3947651 16.796745 180-189
TGGAG 4686365 2.3891842 14.558384 25-29
TACCA 2627530 2.3561187 17.220644 230-239
AATAA 1694530 2.3504581 9.2163925 110-119
CACGG 4304525 2.32949 9.776469 120-129
TCATT 2861595 2.3068852 17.08685 150-159
CATTT 2861165 2.3065388 18.117205 150-159
CTGGA 3993505 2.2852693 14.770747 180-189
TCAGC 3555645 2.2838693 14.988555 140-149
TTCAT 2799450 2.2567873 16.352797 140-149
CTACT 2938710 2.240407 8.001997 180-189
TGTAG 3696060 2.2365053 8.465401 210-219
ACTAC 2463115 2.208687 12.916206 230-239
CAGAG 3275975 2.2049718 9.997105 140-149
TAGTC 3245570 2.2044055 28.326658 70-79
CACTA 2442600 2.1902912 16.216173 240-247
TTCAC 2871535 2.1891947 19.129093 200-209
GTGAA 3068080 2.1836185 18.460754 190-199
GATAC 2727505 2.17894 14.738211 150-159
TGGGG 5962695 2.177507 52.93865 15-19
GTCAA 2692400 2.1508956 43.084938 70-79
TTGTC 3721725 2.1491444 13.337075 170-179
ATTTG 2988090 2.14606 15.950019 150-159
TTGGA 3521485 2.1308684 16.765368 170-179
CTCGC 4115795 2.125591 16.642353 100-109
TCTCG 3872015 2.1145146 17.070559 100-109
TCTGC 3848380 2.1016076 12.52105 210-219
GAACT 2617225 2.09084 11.6255865 160-169
ATATA 1771885 2.0895824 10.735116 110-119
AGCTC 3243980 2.0836804 12.868293 140-149
TACAG 2593175 2.0716271 14.23537 160-169
ATCTC 2714190 2.0692382 8.880047 100-109
GAATC 2571245 2.054108 14.667198 190-199
AACCA 1943220 2.049513 15.706014 70-79
GATAT 2423805 2.0475054 8.550849 180-189
GTCTC 3741950 2.043486 12.146345 180-189
GCTGT 4199035 2.0429301 10.381804 140-149
GTTCA 3004560 2.040711 15.26224 140-149
ACACA 1933495 2.039256 13.589572 110-119
GTCCT 3722745 2.0329983 14.431187 130-139
AATAT 1720685 2.0292027 13.314061 110-119
CTCTG 3705980 2.0238426 14.8214655 180-189
CAGGC 3714250 2.0100493 15.576965 140-149
TGAAT 2377230 2.0081615 14.344367 190-199
ACACG 2654615 2.005551 13.6438875 120-129
TTGCA 2920285 1.9834704 14.6104355 150-159
AATCG 2473740 1.9762135 13.46579 190-199
ATGTA 2334175 1.9717907 7.3081994 150-159
ACGGC 3635820 1.9676051 14.675207 120-129
GGGGC 5620125 1.9409542 48.16539 15-19
TGGAA 2712205 1.9303347 9.6523905 170-179
CAGCC 3172725 1.9272487 10.448104 120-129
CCTTC 3142760 1.926436 14.586409 200-209
ATATG 2274885 1.9217056 8.743155 180-189
TACTA 2017195 1.9126918 9.909886 240-247
TTTGC 3310330 1.9115806 12.541347 150-159
GGGCG 5494530 1.897579 60.903473 20-24
GAGTC 3312650 1.8956524 12.753093 210-219
CATAG 2350875 1.8780594 8.63668 210-219
TCGGC 4070530 1.8728684 7.941758 130-139
AGAAC 1929550 1.813072 12.266238 160-169
AGTAT 2129740 1.7990946 12.483461 220-229
AAAAA 1101195 1.7965807 8.228342 120-129
CTGTT 3109835 1.7958028 13.007745 140-149
GACGT 3121540 1.7862904 10.340345 170-179
GTCAC 2738905 1.7592595 9.13237 140-149
GTTGT 3418965 1.7589208 7.4129653 210-219
GCAGA 2587825 1.7417963 14.699847 150-159
CTCCA 2412370 1.7392681 8.044004 150-159
ATTGT 2418150 1.7367264 7.8795605 170-179
CGTAG 3008210 1.7214379 9.772529 210-219
TGGTT 3336220 1.7163519 18.488947 10-14
TGCAG 2998360 1.7158012 13.791097 150-159
GGTCA 2989435 1.710694 7.9454255 130-139
CCAGA 2264300 1.7106694 24.287663 60-69
AACTG 2132240 1.7033969 11.485736 160-169
AGTCT 2506365 1.7023342 13.659952 210-219
TTCAG 2485320 1.6880407 9.874632 150-159
ATAGT 1995440 1.6856449 7.815561 220-229
GAGAA 1999020 1.6734258 11.253452 160-169
TGACT 2459735 1.6706631 12.261455 190-199
CAAAG 1758055 1.6519294 30.84459 70-79
CACCA 1943950 1.6484923 13.900427 230-239
CAAAA 1250515 1.6403801 21.044142 70-79
GTCTG 3370815 1.6399814 9.3886 210-219
TCAGA 2043475 1.6324846 10.183679 130-139
TCCCA 2251195 1.6230643 10.974945 240-247
ACTGA 2012780 1.6079631 9.319992 180-189
CTGGT 3296145 1.6036525 7.4327617 160-169
TCTGG 3263810 1.5879207 12.142866 180-189
AATAG 1596180 1.5859466 10.180833 240-247
TTGTA 2207870 1.5857023 9.590453 210-219
GAACC 2098335 1.5852836 13.065135 70-79
GCTCC 3066980 1.5839336 6.337879 150-159
CCTTG 2895565 1.5812734 20.221384 20-24
TAGTT 2194715 1.5762544 10.6216545 220-229
TGCTC 2875690 1.5704198 5.202482 150-159
GTGCT 3218705 1.5659763 8.652531 220-229
TGTGC 3216175 1.5647454 8.436959 220-229
ATAGA 1573910 1.563819 10.863041 240-247
GCTCA 2433750 1.5632516 11.515184 140-149
GGCCG 4016990 1.5571835 13.37408 120-129
GGACG 3215280 1.5501893 7.7014403 170-179
TTGGT 3000725 1.5437528 22.133875 25-29
CTGTG 3151075 1.5330725 5.351304 210-219
GTCCC 2938590 1.5176269 18.293522 50-59
ACCAT 1673085 1.5002633 11.186641 45-49
GCTCT 2733335 1.4926795 10.137954 130-139
CCCAC 2168850 1.478783 10.726997 240-247
CCACC 2141785 1.4603294 10.015675 230-239
AATTG 1719520 1.452562 9.85278 170-179
CCTCG 2809340 1.450876 9.662818 130-139
TATAC 1522100 1.4432458 6.780488 110-119
TGATA 1701165 1.4370565 8.066386 180-189
AAATA 1035965 1.4369721 5.0183105 9
CGACT 2234650 1.4353654 9.022002 190-199
TGGAC 2495490 1.4280356 11.011034 170-179
CCATC 1972250 1.4219508 11.092154 240-247
TCGAC 2189950 1.4066535 9.7645445 190-199
CTCTT 2169280 1.406069 8.780592 200-209
CACTG 2165710 1.3910837 7.820882 230-239
GGGAG 3235570 1.3897829 6.6831355 150-159
CATGT 2024735 1.375209 6.876786 150-159
CTGCT 2506300 1.3686951 6.083304 90-99
CAGAC 1806535 1.36483 13.87641 60-69
GAGGG 3177405 1.364799 8.522482 200-209
GCTGA 2380060 1.3619812 18.624937 30-34
CCTCA 1869570 1.3479207 7.4531054 130-139
AGATC 1685285 1.3463349 7.217379 130-139
GAATT 1591325 1.3442694 10.884122 170-179
CTGAT 1975080 1.3414831 7.7072973 180-189
CTTGA 1969220 1.337503 8.965081 200-209
GCGTA 2318120 1.3265362 10.3710165 210-219
ACGGA 1967815 1.324484 9.549359 200-209
CTCGA 2058490 1.3222138 8.801571 190-199
AAAGT 1319900 1.3114378 22.935265 70-79
GGAAT 1834795 1.3058631 9.076967 170-179
ACGTG 2270875 1.2995005 9.780645 170-179
ACTTC 1702315 1.2978072 6.8748302 220-229
ACTAT 1359800 1.289354 6.6156945 230-239
ATCGG 2251910 1.2886477 7.938632 190-199
TATGG 2127540 1.2873852 6.3862686 190-199
AGCCG 2377075 1.2864072 9.244356 120-129
AAGTA 1290325 1.2820524 16.577263 70-79
ATAAC 1144350 1.2762485 8.157112 80-89
TCCAC 1760110 1.2690023 9.962918 240-247
GTAGG 2489000 1.2689322 5.0012226 160-169
GTCCA 1968600 1.2644755 15.541262 70-79
GTGTT 2430590 1.2504414 7.70924 160-169
CCATG 1942185 1.2475085 6.819933 70-79
GGAGT 2442090 1.2450167 7.2790117 210-219
GTGGA 2429045 1.2383661 18.850666 25-29
GGCTC 2690005 1.237683 8.324547 130-139
ACTCG 1923915 1.2357733 9.008958 190-199
GATCT 1809760 1.229197 5.591218 130-139
ACTGG 2144000 1.2268965 7.6315036 160-169
AGCTT 1794195 1.2186253 6.6982527 150-159
AGAGC 1800860 1.212111 7.6170435 140-149
TTGAG 1992300 1.2055509 8.611903 200-209
ACCGT 1863630 1.197051 16.20588 45-49
TTCCA 1552495 1.1835877 8.259937 230-239
ATAAA 847690 1.1758186 5.48795 7
TATCA 1237145 1.1730531 6.8172655 70-79
GCATA 1454470 1.1619422 8.335213 210-219
GGAGG 2694420 1.1573412 14.350022 30-34
TTGGC 2373620 1.1548222 7.5985584 60-69
ATATC 1209930 1.1472481 7.3107104 70-79
TCACG 1770210 1.1370449 9.788842 200-209
GTGGT 2621665 1.1363482 9.387811 50-59
ATACC 1263110 1.1326368 6.808716 70-79
TCTAC 1485185 1.1322721 7.8315907 180-189
TGGTC 2325850 1.1315812 10.714561 50-59
GTCTA 1660990 1.128152 7.9352355 180-189
AGGTT 1860060 1.1255318 23.324282 10-14
ACTGT 1651725 1.121859 5.8964343 230-239
GCTTC 2029785 1.1084695 5.9809666 150-159
AGTTC 1628925 1.1063731 5.3726487 160-169
TGCCC 2137245 1.1037744 5.8937306 60-69
CCATA 1226535 1.0998398 9.43788 70-79
GAAGA 1311695 1.0980502 17.489685 35-39
GAGCT 1916140 1.0965044 6.6953187 140-149
TACTG 1607910 1.0920995 6.9313087 180-189
CAATA 977615 1.0902956 6.817187 100-109
AAAAG 928875 1.0855334 13.902032 70-79
CTGAA 1351140 1.0793943 13.967649 35-39
CGGCT 2343955 1.078464 7.8505063 130-139
TCTGT 1865185 1.0770682 6.6795015 210-219
AGTTG 1775690 1.0744791 5.0949583 220-229
ATCCA 1197410 1.0737233 16.156715 240-247
CTCGG 2332215 1.0730622 8.441814 130-139
AGGGG 2447510 1.0512853 5.032461 60-69
TATGT 1452335 1.0430737 7.699004 220-229
GTCCG 2266675 1.0429071 5.253836 130-139
CTACC 1444795 1.0416669 9.2317915 230-239
GGGGT 2851185 1.0412196 7.8014092 10-14
CTGCG 2247395 1.0340362 6.9141088 210-219
GCGGT 2522460 1.0339772 5.94411 130-139
ATACG 1283655 1.025482 5.28131 120-129
CTGCC 1962155 1.0133497 5.8030157 200-209
TAGAT 1197995 1.0120044 8.340566 240-247
CATGG 1760425 1.007397 5.289029 190-199
CGAAC 1333330 1.0073255 13.8204565 70-79
AAAAT 724615 1.0051031 5.5349417 8
CAGCG 1847810 0.99998367 6.5645237 160-169
AGAAA 851870 0.99554116 5.202678 6
TATAA 843510 0.9947506 6.8685493 240-247
AACTT 1042915 0.9888855 6.069858 200-209
AGAGA 1179955 0.9877676 12.346983 35-39
CTTCT 1523665 0.9875987 5.5365562 210-219
TGCTA 1446310 0.98234034 5.24982 220-229
ATGGG 1912285 0.9749135 27.28695 15-19
AGCTG 1683820 0.9635602 5.240972 90-99
TGCGT 1975995 0.96136844 8.310491 210-219
ACCCA 1113575 0.9443247 13.45882 240-247
AAGAG 1124130 0.9410351 13.018319 35-39
CGGAG 1947370 0.9388893 6.7981534 210-219
ACAAT 841275 0.9382409 7.8867135 100-109
TCGAA 1161270 0.9277116 17.370562 70-79
TGTAA 1095370 0.92531216 7.274145 230-239
TAGGC 1610640 0.92168313 5.7015767 160-169
TCCCC 1588965 0.92110693 5.0349007 80-89
AGTTT 1262310 0.90659684 5.11753 220-229
CCATT 1186905 0.90487003 5.586599 50-59
GAGTT 1492160 0.90291363 5.100399 160-169
ACTAA 800260 0.89249843 13.084047 240-247
CCCAA 1043010 0.88448465 10.374004 240-247
TTCTG 1524850 0.88053876 5.616075 210-219
TGAAC 1094215 0.8741429 6.654708 190-199
CTGTA 1284105 0.8721698 5.1436396 230-239
CGTGG 2122640 0.8700876 6.4581547 50-59
AGCGT 1517260 0.8682468 6.790557 160-169
TGCAT 1277485 0.8676736 6.3084216 210-219
CGGTC 1876845 0.86354464 5.808182 130-139
CATCA 959490 0.8603793 5.9091153 230-239
CCAGC 1414400 0.8591668 5.8278303 80-89
TGAAG 1206330 0.85857105 9.083694 35-39
ACGTC 1321065 0.84854937 10.07355 70-79
CCAAT 932545 0.8362175 11.245083 240-247
CTTCC 1350750 0.8279771 5.786693 230-239
GTATG 1367285 0.8273513 5.3859525 220-229
ATCAA 740505 0.8258561 12.563675 70-79
TAACC 908180 0.8143694 6.635537 80-89
CGTCC 1562025 0.8067036 8.852718 70-79
GGTGG 2177575 0.7952251 18.820456 25-29
GAGGC 1629720 0.7857399 12.229601 30-34
AAAGG 921795 0.7716559 8.934379 10-14
AACCG 1010655 0.7635458 5.767373 190-199
AGCCA 994210 0.7511217 5.8850336 80-89
TGAGA 1053280 0.7496421 5.737408 240-247
GGGCC 1931225 0.74863803 14.212796 15-19
GTCGA 1301345 0.74469024 12.540504 70-79
GGTGC 1810535 0.74215317 5.12816 220-229
AAAGA 632130 0.7387412 6.224307 8
GACCG 1347490 0.7292243 14.977041 45-49
ATGAT 862265 0.728397 7.5859766 240-247
CTAAT 758290 0.7190058 10.02653 240-247
AAGGT 1009815 0.7187071 10.071751 10-14
GTAGC 1241940 0.7106959 5.523958 80-89
AATCC 792445 0.7105893 9.821605 240-247
TGGGC 1701195 0.6973338 15.472333 15-19
GGTCC 1509760 0.6946472 10.861744 50-59
GGAAG 1131190 0.67831016 6.016169 30-34
CATAC 746315 0.66922426 8.026169 70-79
AGACC 880695 0.6653615 5.0960484 240-247
AAAGC 701445 0.6591021 9.350294 70-79
CATTG 959900 0.6519683 5.065037 50-59
TAGGT 1057320 0.6397897 5.498887 10-14
TATGA 754580 0.63743025 6.1305356 240-247
ATAGG 869405 0.6187742 5.4366126 10-14
CAATC 685855 0.6150095 9.581213 240-247
AGGAT 862485 0.61384916 5.0277705 10-14
GAGGT 1171035 0.59701234 6.567947 10-14
AGAAG 710335 0.59463775 5.4962573 9
GGGTC 1447860 0.5934897 6.092489 70-79
AATGG 819270 0.5830921 6.8226833 10-14
GAAAA 487270 0.56944996 5.8767986 8
AAATG 570995 0.56733423 5.452774 9
CGATG 988090 0.5654311 12.278922 15-19
TAAAA 390720 0.541962 5.9839907 8
CATCC 745305 0.5373492 7.211234 240-247
AAGAA 452065 0.5283075 5.6259766 8
AAGGG 878290 0.5266604 6.572194 10-14
GAAGG 868590 0.52084386 5.5066113 10-14
AAGCA 539595 0.50702214 6.2924094 70-79
GGTCG 1234310 0.50595385 6.17019 70-79
TGATC 728780 0.49499065 5.758175 240-247
ATAAG 479505 0.4764307 6.2293587 9
GATCC 723490 0.4647137 5.7508607 240-247
CGGTT 922235 0.4486892 8.087638 10-14
TAAGG 626325 0.44576895 6.1923566 10-14
ACGAT 537260 0.42920452 5.540604 10-14
TAAAG 402560 0.39997905 5.2955503 9
AAGCT 490670 0.39198485 7.7206864 30-34