FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11395985
Filtered Sequences 0
Sequence length 50-251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 51189 0.44918451542363386 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 42851 0.37601839595260966 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 40336 0.3539492198348804 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38574 0.3384876340219823 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36999 0.3246669770098855 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36662 0.32170979516031306 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 33755 0.29620081107512863 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33507 0.29402460603449376 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33500 0.29396318089221773 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 33239 0.29167290058735595 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 32164 0.2822397537378296 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31377 0.27533381274194374 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30624 0.26872622243711275 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30255 0.2654882399371358 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29583 0.2595914262786411 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29581 0.25957387623799083 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29423 0.25818742302661857 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29010 0.2545633396323354 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27656 0.24268196211209475 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27634 0.24248891166494166 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27157 0.23830322696984946 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27081 0.23763632542513877 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26820 0.235346045120277 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26612 0.23352084089264774 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26444 0.23204663747802404 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25345 0.22240289014069428 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25274 0.2217798636976093 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24189 0.2122589666448315 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23909 0.20980196095379205 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23611 0.20718700489690012 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 23538 0.20654642841316484 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23009 0.20190444266116533 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22671 0.1989384857912677 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22576 0.19810485886037932 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22392 0.1964902551205534 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22354 0.19615680434819804 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22118 0.19408589955146482 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 21663 0.19009326530352574 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21331 0.187179958555579 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 20481 0.1797211912792093 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20338 0.17846636337271415 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20212 0.17736071081174642 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19750 0.17330665142153134 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18884 0.1657074838199594 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18677 0.16389105461265527 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18613 0.16332945331184623 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18108 0.1588980680476501 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17992 0.15788016568993377 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17734 0.15561621044604743 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17615 0.15457198302735567 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17395 0.1526414785558247 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17305 0.15185172672656203 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17264 0.15149195089323125 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16871 0.14804336790545092 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16728 0.14678853999895577 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15514 0.1361356653242348 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15370 0.13487206239741453 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15271 0.1340033353852256 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15027 0.13186223042589124 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14996 0.13159020479581188 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14577 0.12791347127957786 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14077 0.12352596111700745 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13893 0.12191135737718152 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13647 0.11975270237719689 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 13032 0.11435606487723528 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12990 0.11398751402357934 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12186 0.10693239768216614 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 23353935 8.18157 68.46891 35-39
TGGAT 17662460 7.6456933 81.17638 35-39
TATTA 10067310 6.867046 143.55444 240-247
TCACC 17824565 6.828437 77.897285 160-169
TCTCC 15794715 6.411026 88.697365 20-24
TCCCT 14837235 6.0223885 73.26397 15-19
AGTGG 17874280 5.9881434 40.859356 100-109
CTGTG 15491095 5.7500553 88.81016 25-29
CTGAG 15737665 5.513366 51.811283 15-19
TGGGT 14624170 5.1909666 46.307076 100-109
TGTAT 9213115 5.1531677 67.25378 240-247
TTCAC 10650120 5.041341 76.7136 40-44
TTACT 8487180 4.9641414 123.88345 240-247
CTCCA 12830720 4.9153385 43.0107 80-89
CCTGA 13326700 4.8821607 53.6491 15-19
CTCTG 12563650 4.8766174 74.68227 35-39
ATTCA 8616065 4.7563777 116.70038 40-44
ATTAC 8367165 4.6189756 118.69922 240-247
AAGGG 14499550 4.584635 40.459667 90-99
TCTGG 12324320 4.5745974 136.97655 35-39
TGAGA 11044740 4.5124025 98.26506 15-19
GAAGG 13979685 4.420258 32.590446 150-159
CCAGG 15512335 4.398094 54.47042 80-89
ACACG 12664750 4.3789773 56.413136 230-239
GACAC 12576850 4.3485856 53.576614 230-239
GAGAC 13056845 4.3171926 87.74251 8
ACCAT 9643230 4.3082476 70.08235 160-169
CCCTG 13695400 4.302178 86.26999 10-14
TGGAG 12775380 4.2799377 39.199093 90-99
TCCAG 11654215 4.2694554 42.35425 80-89
CAGCC 14206855 4.2120957 57.557358 30-34
TCAGT 9302800 4.211068 96.42596 7
GGGTC 14603635 4.195163 128.65443 8
AGCCT 11445885 4.193135 52.98696 30-34
ACTAC 9283565 4.147563 35.60747 130-139
CACCA 11438435 4.1357627 60.020363 160-169
TACTG 9112375 4.124869 88.71186 240-247
TACTA 7427180 4.10007 29.77769 130-139
GACTC 11066960 4.054318 54.033558 20-24
GATTC 8941175 4.047373 60.863266 40-44
CTGGG 14016670 4.0265465 135.85313 3
AGACT 9413890 4.0219293 72.26754 15-19
CACCT 10482045 4.015581 44.633236 40-44
TGAAG 9447300 3.8597577 41.839073 150-159
ACTGG 10771225 3.7734766 45.08784 60-69
CAAGA 9335725 3.764436 46.699146 180-189
ACTCT 7872035 3.7263064 71.17755 20-24
GCTGG 12957190 3.7221913 33.73828 90-99
CCTGT 9460915 3.6722815 63.119205 25-29
ACTGT 7935350 3.5920694 76.60962 240-247
TCCTG 9211800 3.575587 65.093864 20-24
GTATT 6361600 3.558231 76.38537 240-247
GAGTG 10596870 3.5501056 39.592205 100-109
CTCCT 8639965 3.5069354 95.728294 20-24
GGACA 10595460 3.5033455 31.490936 220-229
GGTCC 11539700 3.4665325 144.99611 9
GGATT 8007660 3.4663417 58.08682 40-44
TATCT 5767685 3.373512 53.657085 200-209
AGACA 8284305 3.340473 39.17425 170-179
CATCT 7034080 3.329652 35.00725 170-179
CTGCA 9005030 3.2989416 45.921505 25-29
GTCTC 8481210 3.2920065 33.590878 30-34
GTCCC 10381865 3.261287 77.239494 10-14
CCATC 8488325 3.2518039 35.347687 45-49
AGCTG 9071660 3.178069 36.20263 60-69
GGAGT 9473750 3.173844 37.575462 90-99
CCTCA 8225070 3.1509533 82.05372 5
AGTAG 7440270 3.039772 29.431591 50-59
AGGGA 9564585 3.0242412 41.24472 80-89
AAGAA 6379295 2.9998553 39.313835 180-189
GTGAA 7250555 2.9622626 40.886406 150-159
GGCTG 10294515 2.9572892 35.01902 90-99
GGAAG 9174070 2.9007633 30.885067 80-89
GACAA 7173470 2.892552 26.785341 180-189
ATCTC 6085715 2.880734 51.109425 170-179
CAGGG 10572195 2.8664184 36.79809 80-89
ACCTT 6049410 2.8635485 72.28843 40-44
TGTGC 7546015 2.8009644 58.470955 25-29
CTATG 6133200 2.7762957 27.21831 50-59
GAGCT 7918010 2.7739115 29.387304 60-69
GGGAA 8682570 2.7453551 34.598618 80-89
CGCCA 9221835 2.7341204 41.200974 70-79
ATCTG 5969230 2.702072 39.829582 200-209
GAACA 6645735 2.6797538 39.18296 210-219
ATGAA 5348640 2.6649213 30.829437 210-219
GAGTC 7545530 2.643421 27.799795 150-159
AGGGC 9664215 2.6202397 34.019268 150-159
AGGCT 7473605 2.6182234 20.753113 80-89
TGCAA 6082875 2.5988078 38.557076 200-209
CAGTG 7417500 2.5985682 74.56139 8
AGAAC 6390215 2.5767207 32.892036 190-199
CCCTC 7798175 2.5616484 26.804161 20-24
ATCAG 5966375 2.549035 27.262733 45-49
CCAAG 7350785 2.5416152 40.695007 180-189
CACTG 6926065 2.5373242 20.0186 60-69
CTACA 5606955 2.5049853 20.293327 190-199
CTCTC 6142240 2.4931164 100.46769 20-24
GTGGA 7379490 2.4722362 26.725845 100-109
CTACT 5196085 2.4596186 22.811674 50-59
GCAGC 8668075 2.4575932 35.457626 30-34
CTTCA 5182980 2.4534154 51.57535 45-49
GGGCT 8521025 2.4478214 43.18754 90-99
CTGTC 6244675 2.423889 47.984272 30-34
GTGTA 5556895 2.4054587 39.44366 230-239
CAGGC 8456885 2.397716 34.19218 70-79
TATGC 5295320 2.3970153 20.793612 50-59
TATAT 3481155 2.374542 27.239107 240-247
CCGCC 9298540 2.3639553 35.043503 70-79
GCCTC 7507440 2.3583353 71.35026 4
GCTCC 7416050 2.3296266 34.98218 80-89
ACAAC 5512180 2.3242784 22.246744 180-189
TGAAC 5432905 2.3211186 41.99574 210-219
TGGTG 6521510 2.3148625 33.709835 35-39
CACGG 8162400 2.314223 49.960255 230-239
TCCAA 5172045 2.3106835 44.098507 180-189
GTATC 5062500 2.2916255 25.054523 200-209
CAAAT 4382585 2.2834122 38.5033 200-209
ACAGC 6598035 2.281344 29.575598 210-219
CAGTA 5335535 2.279519 21.819916 45-49
TCTGT 4750820 2.2785604 25.273252 210-219
GTGGT 6300190 2.236303 30.730598 120-129
CCTTC 5508335 2.235816 55.997696 45-49
GGGGG 10508700 2.2341988 96.62631 5
TGCAG 6307860 2.2098286 44.839367 30-34
TACAT 3997900 2.2069845 19.018408 190-199
GTAGT 5095910 2.2059083 25.47841 120-129
AAATG 4399565 2.1920514 36.322445 200-209
GTGGG 7957365 2.18597 30.647482 100-109
CCTCT 5384785 2.1856675 50.160748 35-39
ACGGC 7707815 2.185338 54.78646 230-239
AATGA 4382060 2.1833298 29.18917 210-219
GCTAT 4775260 2.1616015 33.154045 50-59
TATCA 3908950 2.1578808 17.340828 170-179
GGCCG 9240295 2.1482484 26.652832 230-239
GCAGA 6422420 2.1235466 25.441515 140-149
TGCAC 5789430 2.1209247 26.72164 25-29
GCCTG 6984550 2.098163 33.02654 210-219
CTCAC 5462370 2.092587 54.736195 20-24
CAGAG 6322040 2.0903566 34.59672 170-179
AACTA 4006255 2.0873368 36.69082 130-139
TCCGC 6633890 2.0839243 43.206898 70-79
TCTAT 3555095 2.0793707 19.724104 110-119
GGCTC 6910870 2.0760295 19.772947 40-44
TTCAG 4564055 2.0659962 48.377506 45-49
ATGGA 4959200 2.0261142 12.527177 100-109
GCCAG 7112385 2.0165203 36.387856 70-79
GGTCT 5422715 2.012828 35.63054 15-19
AGAGT 4908640 2.0054579 30.86006 150-159
AGCAC 5787790 2.0011926 20.868355 130-139
AACAG 4959340 1.9997501 37.37557 210-219
ATATC 3611610 1.9937385 13.926041 170-179
TCTCT 3973385 1.9928087 32.85323 35-39
CATAT 3600495 1.9876027 14.078311 160-169
TGGTA 4569570 1.9780672 30.758583 120-129
CCAGA 5708265 1.9736958 36.42872 170-179
ACTAT 3572440 1.9721153 17.461279 130-139
TGGGG 7148810 1.9638517 125.9287 4
GGATC 5581470 1.9553529 18.436533 70-79
GATGG 5832620 1.9540124 21.16165 100-109
GCTGT 5236070 1.9435484 21.96103 230-239
TCCAT 4090575 1.9363146 26.943563 45-49
AGAGA 5005360 1.9300754 30.184296 170-179
CAGAC 5581400 1.929831 27.794643 140-149
ACCCT 5009025 1.9189144 43.148804 10-14
TGGGA 5704960 1.9112444 16.78167 100-109
CTGTA 4214610 1.907814 25.695896 190-199
GTCAC 5200095 1.9050252 38.79223 160-169
GCACA 5502475 1.9025416 18.714584 130-139
TGAGC 5418890 1.8983961 17.855371 200-209
CCTGC 6037660 1.8966286 49.570904 20-24
TAGTA 3583575 1.8917811 17.959185 120-129
TGTGA 4366450 1.8901409 19.18512 210-219
GGTGG 6828100 1.8757493 17.799635 40-44
ACCAG 5409705 1.8704654 14.495855 190-199
TTCCA 3946185 1.8679662 23.670588 180-189
ATACT 3361955 1.85592 27.575428 130-139
TCAGC 5061325 1.8541876 12.084244 45-49
CTATT 3166700 1.8521988 18.874083 110-119
CAGTT 4085595 1.849413 25.060274 190-199
CAAGG 5592940 1.8492827 17.470087 80-89
CACAG 5300795 1.8328085 16.423231 140-149
TGTCT 3819560 1.831915 49.288975 30-34
AGTCA 4242890 1.8127046 29.796593 160-169
CATGA 4227360 1.8060696 23.91444 60-69
CCGTG 6007505 1.8046578 21.20278 230-239
TAGTG 4168860 1.8046086 26.242887 120-129
ACCTG 4900135 1.7951363 30.56072 25-29
GTGCA 5115400 1.7920749 51.824852 25-29
CCTGG 5934195 1.7826355 35.159927 2
TACAA 3417590 1.780631 17.619923 130-139
ATGCA 4143185 1.7701073 17.094007 60-69
TTCTG 3681330 1.7656181 42.278202 30-34
GCCGA 6224085 1.7646675 21.181067 220-229
GGATG 5252660 1.7597175 22.746048 100-109
CTCAG 4783755 1.7525015 78.152855 6
AGCTA 4062540 1.735653 25.761902 50-59
AGTTC 3821940 1.7300652 22.039682 190-199
GCAAA 4289815 1.7297783 34.98346 200-209
ATTGG 3969400 1.7182666 20.101286 100-109
AGGAC 5179955 1.7127309 23.293852 220-229
CGATT 3737730 1.6919461 37.117237 160-169
AACAC 3999865 1.6865922 24.361074 190-199
AGGGG 6489015 1.6824429 33.625496 90-99
GGGGC 7505185 1.6685804 29.19147 90-99
GCTGA 4752080 1.6647933 27.222599 200-209
TCTCA 3511510 1.6622084 27.01752 100-109
TGACC 4496290 1.6471903 14.735642 210-219
GGGCC 7041260 1.6370014 28.087284 2
GAGGA 5159850 1.6315011 30.277658 220-229
GCACT 4453370 1.6314667 25.734814 25-29
GGAGC 6013445 1.6304137 13.476483 200-209
CTGAA 3796785 1.6221136 23.743954 200-209
AGTTA 3049705 1.6099494 16.24862 50-59
AGTGA 3938805 1.6092252 86.76192 9
GTCCG 5330480 1.6012791 35.377598 70-79
CAGCT 4355610 1.5956529 12.551223 50-59
ACAAT 3031015 1.5792178 24.40624 180-189
GGCTT 4229770 1.5700256 21.323406 25-29
TCTGC 4044605 1.5699252 35.98984 200-209
CCATG 4255450 1.5589597 16.217287 160-169
CGTCC 4933805 1.5498713 19.311258 180-189
TTAGT 2744675 1.535178 25.73836 110-119
CATCA 3415745 1.5260319 31.878435 45-49
TACGC 4123435 1.510597 25.733875 140-149
CTTTA 2566975 1.5014206 41.868652 45-49
ACTCC 3912855 1.4989811 17.764107 140-149
GTTCT 3099520 1.4865737 22.930864 190-199
TCTGA 3273345 1.4817345 23.551313 220-229
CTCCC 4506230 1.4802667 13.863245 190-199
CTGGT 3976365 1.4759654 31.351273 35-39
GTTAC 3259485 1.4754605 15.266479 50-59
GAGCA 4414315 1.4595752 7.9620223 200-209
GGGGT 5292055 1.453782 120.620346 7
CTCAA 3246780 1.4505444 19.129913 150-159
TTCTC 2891045 1.4499725 21.5206 190-199
GGCAG 5291335 1.4346293 13.912259 150-159
TAGCA 3357505 1.4344385 11.68159 130-139
CAACT 3190830 1.425548 17.31899 130-139
CTACG 3883945 1.422861 16.729645 130-139
ATACA 2709045 1.4114652 23.14051 35-39
GAACT 3294495 1.4075186 16.11705 190-199
GTCCA 3806670 1.3945519 29.522821 180-189
CAACC 3854520 1.3936679 15.780135 140-149
TGGAA 3409190 1.3928474 19.81127 120-129
AACCC 3849575 1.3918799 20.506193 140-149
GCCGT 4616245 1.3867227 31.417252 230-239
ATCTA 2494225 1.3769017 20.690287 110-119
GGCCT 4580685 1.3760405 68.16886 3
GTGGC 4693470 1.3482856 14.07578 40-44
CCATA 3013785 1.3464506 14.456493 160-169
AGCAG 4056650 1.3413147 12.8137455 50-59
TCAAG 3136500 1.3400178 23.383453 150-159
GTGAC 3809080 1.3344325 15.359295 210-219
ACAGT 3122250 1.3339298 12.379414 120-129
CAATT 2416180 1.3338182 25.096294 180-189
TCCGT 3428795 1.3308969 14.178337 150-159
CGACA 3842620 1.3286284 16.768797 70-79
TCACT 2804255 1.327422 14.628056 190-199
TGTCC 3415220 1.3256276 53.302147 15-19
CACGT 3616060 1.324723 17.34501 180-189
ACAGA 3271965 1.3193516 21.126907 140-149
CCTTT 2626215 1.3171499 47.643353 45-49
AAGCT 3082675 1.3170218 16.709969 200-209
ACATA 2511130 1.3083477 21.480736 130-139
AAGAG 3373815 1.3009486 18.969374 150-159
AATTC 2354980 1.3000336 25.031673 180-189
TTACA 2348480 1.2964454 9.386472 120-129
CGGCC 5305195 1.2897719 33.75039 230-239
ACATG 3009205 1.2856332 14.935943 190-199
ATTAT 1860580 1.2691263 10.007178 50-59
GATTG 2931165 1.2688373 23.51186 100-109
ATCCG 3462545 1.2684835 23.904345 70-79
AGACC 3668555 1.2684436 99.84294 9
GTGCG 4398460 1.2635386 18.78581 240-247
CCAGT 3441985 1.2609516 19.196291 190-199
TGGAC 3592000 1.258383 16.03829 80-89
CCTAC 3282345 1.257438 17.768066 190-199
CTTCT 2503125 1.2554156 23.130905 30-34
TGCGA 3552260 1.244461 15.851952 240-247
AACCA 2944060 1.241399 18.34814 190-199
AGTCG 3535645 1.2386403 15.851472 150-159
GAGAG 3915335 1.2379957 29.075127 220-229
TACCA 2752535 1.2297335 10.688073 45-49
TGAGT 2824310 1.2225822 21.216309 90-99
ACGTC 3328270 1.2192928 19.176395 180-189
GATCC 3326300 1.2185711 23.760717 70-79
ACGCA 3512690 1.2145514 22.000486 140-149
ATTAG 2291180 1.2095217 25.92539 110-119
GAAGT 2956150 1.207755 14.045321 140-149
AAGGC 3633590 1.2014316 29.390543 25-29
CTTCG 3087345 1.198362 103.78035 3
ATCAT 2166205 1.1958231 15.853269 110-119
GGACT 3408645 1.1941485 12.921319 90-99
GTCGA 3407810 1.193856 12.355945 150-159
ACACA 2811215 1.1853834 8.010921 130-139
GCAAG 3578145 1.183099 17.390663 25-29
TCGGA 3362895 1.178121 93.148575 5
TTATT 1628010 1.176595 21.275417 240-247
CCGAT 3189925 1.1686108 25.283583 160-169
ATATT 1712490 1.1681122 28.88061 240-247
GGGTG 4243975 1.1658636 14.465423 60-69
AGGTC 3299450 1.1558943 18.25353 10-14
AGGCC 4061735 1.1515929 13.961963 2
CGTGT 3083390 1.1445068 25.17167 230-239
AGAGC 3459730 1.1439456 27.61657 220-229
TTGGG 3211500 1.1399478 14.624747 100-109
TCGAG 3241685 1.1356575 11.946079 150-159
ATTCC 2397730 1.1349895 23.159845 180-189
CGGCT 3777530 1.1347721 24.131193 230-239
TTCGG 3052920 1.1331968 99.36594 4
GCTTC 2915410 1.1316249 26.743704 2
TGTGT 2465050 1.1305891 16.356966 240-247
CTTGA 2482870 1.1239127 22.550837 90-99
ACAAG 2784480 1.1227833 17.98765 80-89
ATGGG 3321260 1.1126703 20.171402 100-109
TTGAG 2567455 1.1113954 20.9397 90-99
ATCAA 2123020 1.1061348 12.619957 110-119
GCCCC 4326685 1.0999674 12.390963 80-89
CGCTG 3655110 1.0979972 12.6874075 190-199
GACCC 3671535 1.0885489 42.369446 10-14
CACAT 2431685 1.0863892 14.138503 130-139
GAACC 3140435 1.0858402 10.631577 190-199
CGCAG 3829215 1.0856682 17.066261 140-149
GCACC 3654905 1.0836184 11.238074 130-139
CTATA 1953920 1.078634 13.5939 50-59
TAGAC 2524060 1.0783628 17.213394 170-179
CAGCA 3112335 1.0761243 12.800453 45-49
ATGAG 2625880 1.0728208 20.520824 60-69
TACAG 2510075 1.072388 8.798391 120-129
CTCCG 3411655 1.0717137 10.221112 140-149
ATGGT 2467105 1.067956 9.471313 120-129
AGCTC 2906800 1.0648897 12.804867 210-219
TTTCA 1813840 1.060913 7.996682 100-109
TGGCT 2850795 1.0581712 30.831959 40-44
GATCT 2336985 1.0578754 10.335111 220-229
CGTGA 3018475 1.0574604 21.359394 150-159
CCCCT 3215165 1.0561602 9.493958 70-79
GGAGA 3318075 1.0491474 83.74383 7
GACAG 3156920 1.0438226 16.06842 70-79
GCCAA 3012300 1.0415361 16.210999 180-189
GTAGC 2969035 1.04014 11.426653 50-59
TAGTT 1853790 1.0368795 14.534376 50-59
GGGAG 3986305 1.0335513 14.516077 5
AAGGT 2528700 1.0331173 21.130676 15-19
AAACT 1971730 1.0273098 17.193207 130-139
TTATA 1499365 1.0227368 13.402749 110-119
GAAGC 3089105 1.0214 13.101934 200-209
ATGAT 1933320 1.020606 9.246769 120-129
ACACC 2812640 1.0169582 13.412188 40-44
GATAC 2363460 1.0097492 24.8065 35-39
AACTC 2256800 1.0082569 18.093527 190-199
ACTCA 2251570 1.0059204 13.740182 190-199
GTATA 1896845 1.0013508 10.900882 240-247
TTTAG 1783625 0.9976343 29.91054 45-49
ATGAC 2318905 0.9907138 11.410624 160-169
CCGTC 3148420 0.9890228 10.482324 140-149
AACTG 2290595 0.97861886 12.708999 60-69
CGAGT 2788095 0.9767515 15.406287 160-169
AGATC 2279585 0.973915 13.103828 210-219
GCAGT 2778790 0.97349167 6.5495453 50-59
GGGAC 3573985 0.96900755 9.903518 90-99
ACAGG 2923680 0.9667028 15.625061 70-79
CATAC 2159405 0.96474445 14.313659 130-139
GTAGA 2349210 0.95978546 16.887945 170-179
ACCAA 2270890 0.9575487 16.958176 130-139
CAGAA 2363420 0.9529998 18.832611 140-149
TCATA 1725980 0.9528029 9.084113 110-119
CCAAC 2626645 0.9497086 13.818063 130-139
TAACA 1821015 0.9487843 9.032671 120-129
TATGG 2187890 0.94708985 11.954023 35-39
AAGTT 1789720 0.9447991 18.1926 140-149
GCCAT 2557560 0.9369476 7.751752 60-69
AGCCC 3156965 0.9359875 11.660742 80-89
TACAC 2083970 0.9310427 22.436012 35-39
GCTCT 2394355 0.9293758 17.583853 210-219
AATCA 1780900 0.92788357 13.797061 110-119
GAGCC 3255015 0.9228697 20.068485 220-229
TGAGG 2746345 0.9200654 13.026415 220-229
GTTAT 1633390 0.9136033 10.766218 110-119
GGTAT 2106070 0.91167176 9.2678795 110-119
CGAGG 3352830 0.90904623 16.274416 220-229
CCCAG 3045825 0.90303624 14.541085 80-89
GATGA 2200905 0.8991944 7.159564 60-69
AGCCG 3170785 0.8989885 21.094145 220-229
TGGTT 1954285 0.89632803 8.9625025 50-59
ACCGC 3009725 0.89233327 11.945824 220-229
GTTTC 1833335 0.8792934 5.546846 100-109
ATAGT 1661790 0.87726444 15.9158945 120-129
AGAAG 2269845 0.8752561 16.318438 140-149
CCCCA 2808635 0.87078005 16.775774 80-89
CGGAG 3209945 0.8703061 71.9472 6
AGTCT 1907965 0.8636723 15.82413 8
GACTG 2462860 0.8628122 12.555798 90-99
TCCTT 1718405 0.86184764 10.24449 45-49
GATCA 2008185 0.85796386 9.40042 110-119
TTATG 1531465 0.85659367 12.230772 50-59
CCGAG 3018020 0.8556761 19.618923 220-229
TGGCA 2435005 0.8530538 5.860227 100-109
CGGAC 2993135 0.8486208 11.077457 220-229
GGTAG 2521565 0.8447609 11.769305 120-129
GGTTC 2273375 0.84384173 11.409289 160-169
TTTAT 1165770 0.842525 11.475567 240-247
CTATC 1767945 0.83687437 8.253962 110-119
ACGCT 2281435 0.83579075 15.770833 190-199
GGTGC 2909010 0.8356666 11.64569 60-69
TCTTC 1666185 0.8356572 45.92582 2
GAGAT 2042165 0.8343402 12.652825 210-219
GCTTG 2243315 0.8326839 16.951576 90-99
TCATC 1753295 0.82993966 10.574111 110-119
GTCTG 2230500 0.8279272 14.184595 1
GCCTA 2249515 0.82409704 17.121721 190-199
ATCCA 1839880 0.82199204 9.775778 180-189
TGATG 1886945 0.81681734 12.612635 120-129
TTACC 1715670 0.81212956 9.704276 45-49
CATGG 2311985 0.80995625 9.068463 200-209
ATATA 1257985 0.80987614 11.501706 130-139
ATATG 1531965 0.80872947 5.6384926 50-59
GTCTA 1779830 0.80566996 9.675994 30-34
TATTT 1102460 0.7967696 10.571412 240-247
CGTAT 1754220 0.7940771 7.766216 240-247
TATAC 1429395 0.7890774 9.485062 130-139
CATTA 1425585 0.78697413 13.037001 110-119
ACATC 1745985 0.7800433 5.7581124 110-119
ACAAA 1575770 0.7748781 13.134048 130-139
GTTCA 1706385 0.77242374 14.030599 160-169
TCAAC 1722135 0.76938784 7.748917 110-119
CCAGC 2553780 0.7571532 6.6578064 80-89
AACGC 2181620 0.7543192 16.001604 180-189
TTGGA 1738920 0.75274056 6.587514 60-69
CCTCC 2275885 0.747613 10.223065 140-149
GGGAT 2229380 0.74687463 13.201691 100-109
TATGA 1406200 0.74233764 5.09921 50-59
GGTTA 1708700 0.73965895 7.986301 50-59
CAACG 2132915 0.737479 16.299099 180-189
GGATA 1802080 0.7362519 16.100405 35-39
ATGCC 1994990 0.73085326 8.065245 50-59
ATCTT 1247670 0.7297606 12.620372 3
ATAAA 1188535 0.7221737 8.008063 130-139
ACGCC 2429770 0.72038627 14.213452 180-189
TGCCA 1966275 0.7203337 7.4161735 60-69
GAAAT 1433665 0.71431327 7.225485 110-119
TATAG 1351160 0.7132819 6.7449684 120-129
TGACT 1570375 0.71085656 5.1464834 2
TCCGG 2358085 0.7083701 11.298854 70-79
AATAC 1354635 0.7057912 11.8387 130-139
GGGCA 2598095 0.70441645 13.911833 150-159
TTTAC 1186795 0.69415504 13.292856 45-49
GACCA 2001055 0.6918869 6.3884587 170-179
TATTG 1228625 0.68720627 6.499283 240-247
GATTA 1295690 0.68399906 5.7973285 50-59
AGGCA 2063570 0.682311 6.6982765 80-89
CATCC 1769850 0.67801434 6.550187 110-119
CACAA 1600810 0.6750012 8.56754 130-139
GCGAC 2370565 0.67210823 7.339936 70-79
CAAAC 1593860 0.6720707 13.894293 130-139
CTGAC 1831670 0.6710219 9.9630165 220-229
CAGTC 1817255 0.665741 6.130781 210-219
CCCCC 2499620 0.66452515 13.09175 80-89
CCCGT 2102675 0.66051966 10.249337 140-149
AGATG 1608850 0.65730643 6.1762996 120-129
AAATA 1079785 0.6560954 13.160291 130-139
AGCAA 1613725 0.65070087 5.0421376 50-59
GCGGA 2387975 0.647447 9.137855 220-229
TTTCT 1043290 0.64654565 9.682217 4
ACTTC 1364030 0.64567727 29.843475 4
GACCG 2264175 0.6419443 9.436289 210-219
TCAAT 1151105 0.6354513 8.270194 110-119
AAGCA 1538285 0.6202812 6.0299573 120-129
AGTAA 1242000 0.61881757 5.6238384 50-59
TAGCT 1363585 0.6172496 12.57204 50-59
CACGC 2066285 0.61261904 12.823812 190-199
AATGG 1496080 0.6112335 6.806009 120-129
CCCTA 1588895 0.60869193 12.119389 110-119
CGCCG 2491145 0.6056345 11.347403 220-229
AATAA 992275 0.60292286 7.420584 130-139
ACCTA 1331115 0.5946942 7.5589085 130-139
GTTCC 1515620 0.5882923 6.955723 140-149
CACCG 1977595 0.586324 9.097492 45-49
TGATT 1045145 0.5845805 6.159529 50-59
GGTGA 1742370 0.58371925 6.797522 120-129
TCCAC 1503580 0.57600856 10.331541 180-189
GGCCC 2355570 0.5726742 10.099553 70-79
CACGA 1653790 0.5718162 8.844896 180-189
GACGA 1721580 0.5692333 10.182094 220-229
TTAGC 1257035 0.56901795 9.546704 45-49
GCCGC 2330640 0.5666133 12.425495 220-229
CCGGC 2327195 0.56577575 9.110151 70-79
TCAGG 1606445 0.5627849 5.4103794 150-159
TTCAA 1012865 0.5591379 5.9082103 200-209
GGGGA 2103870 0.54548204 9.226366 4
TACTT 931930 0.54508466 11.543759 1
CGGTT 1466575 0.54437 11.29221 160-169
ACCCG 1833680 0.5436556 8.962285 140-149
AAGAT 1090695 0.54343086 5.929856 15-19
GCTAC 1478800 0.54174995 7.8618746 50-59
CTTTG 1123580 0.5388849 7.2774386 45-49
TAATA 836820 0.538735 8.038398 130-139
CGGCA 1898620 0.5383013 10.647473 70-79
ACATT 973910 0.5376333 6.715185 110-119
ATTTC 907140 0.5305851 6.739574 240-247
GTCCT 1366885 0.5305604 6.1471305 45-49
CCGTA 1409640 0.51641357 6.8681097 230-239
ATTAA 787350 0.5068868 7.139965 110-119
GGGTT 1426940 0.5065038 6.2091856 100-109
ACGAC 1464265 0.5062859 9.526129 220-229
GGAGG 1936815 0.5021688 5.3902636 6
AAATC 962440 0.50144994 7.7268353 110-119
TATGT 896210 0.50127673 7.063415 140-149
GTAAC 1173135 0.5012025 7.0482836 120-129
TGGCC 1643510 0.49371135 14.800465 2
GAAGA 1279720 0.4934622 6.5725927 15-19
AGTAT 919365 0.48533583 5.4774985 110-119
CCACG 1634470 0.4845931 7.708621 180-189
TATCC 1022920 0.48420942 5.405557 110-119
CTGCG 1610340 0.483747 11.631194 25-29
CATAA 917490 0.4780302 6.38796 110-119
GGTTT 1036060 0.47518638 5.7882333 100-109
AACAT 895935 0.4667996 6.2411466 110-119
CAATC 1040415 0.46482 6.194618 110-119
ATGCT 1017360 0.46052507 6.902216 50-59
ACCCC 1481730 0.4593908 8.717602 140-149
CCGGT 1494115 0.44883296 8.8216505 160-169
TGCTA 985940 0.4463023 6.0933566 50-59
ATTCT 756445 0.44244376 7.6526523 2
TAAAT 677200 0.43597344 8.003846 130-139
CTGTT 898440 0.43090457 5.008742 240-247
GCATG 1226670 0.42973855 8.593389 60-69
TGCGC 1414350 0.42487156 8.605465 25-29
TGACG 1198955 0.42002916 9.005369 220-229
GGTCA 1197045 0.41936004 5.514069 160-169
CGCGG 1798935 0.418229 6.579496 220-229
CTAAC 926895 0.41410327 5.794421 110-119
ATGGC 1174160 0.4113427 6.9685035 1
CACTT 861510 0.40780434 6.7437515 3
CATGC 1091485 0.3998593 7.17158 60-69
GCCTT 1025210 0.39793828 8.955894 1
CCTAA 886565 0.3960853 7.968681 110-119
GCGCT 1314705 0.3949381 7.7782307 25-29
AGCTT 860465 0.3895039 7.145605 1
GGTAA 943715 0.38556108 7.4237065 120-129
TTTGA 683335 0.3822095 6.1611776 45-49
CCGCG 1556010 0.3782892 6.8661675 220-229
ACGAG 1127345 0.372752 7.7167473 180-189
CCGGG 1537395 0.35742432 5.1686416 80-89
GAGGC 1272345 0.34496844 5.701104 35-39
TGGGC 1161390 0.33363062 9.137 1
TTCTT 531770 0.32954744 19.51467 1
TGCTG 876550 0.32536185 13.865009 1
CGAGC 1089165 0.30880266 6.4586544 180-189
ATGTG 698090 0.30218792 5.325045 140-149
GCTTT 619815 0.29727206 5.108099 45-49
CGGGA 1077140 0.29204285 5.0262885 80-89
GACTT 644285 0.2916464 6.708399 1
AACTT 526585 0.29069385 10.6962595 3
CGCTT 740040 0.2872487 5.7419205 1
CTCTT 557695 0.27970597 8.804099 1
CTTTC 557505 0.27961063 5.027831 45-49
TCACA 620050 0.27701598 5.136247 5
GAGGT 795790 0.26660123 6.827987 7
TTGGC 707665 0.26267433 7.300742 1
CTCGG 856475 0.2572856 5.022487 6
GCGAG 947825 0.25698194 5.89541 240-247
TAACT 446905 0.24670762 7.0928926 2
AATCT 436225 0.24081188 7.595994 2
CCCTT 593235 0.24079224 5.203899 3
TTCCT 477730 0.23960036 6.06529 4
GTCTT 495505 0.23765123 11.735615 1
ATTGT 419705 0.23475341 5.461113 240-247
TGTAA 433430 0.22880913 8.068716 25-29
CGGGG 1013285 0.22527725 8.930937 3
CCTCG 682740 0.2144712 5.5102286 5
TGCTT 424390 0.20354347 10.983489 1
TCGGT 508025 0.18857104 5.9082675 7
TCGCA 437605 0.1603141 5.1169786 7
TAGGC 424165 0.14859745 6.7003427 1
TTGCT 227555 0.10913861 5.701892 4
Produced by FastQC (version 0.10.1)