FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_18_SL248347.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_18_SL248347.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5365270
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 6067395 8.848996 62.366493 110-119
ATACA 5553620 7.658939 37.924183 110-119
CACAG 8069805 7.5447187 42.291508 110-119
TCCCT 9593575 7.2794657 107.37036 30-34
CAGTA 6907085 6.829267 39.697994 110-119
AGTAA 5290320 6.5035753 48.21185 110-119
GGAGA 8427055 6.2605343 93.51316 35-39
CCCTG 9605005 6.1432066 70.152794 30-34
GTAAT 5755080 6.017693 40.253124 110-119
GGTGA 9354095 5.910793 101.44595 45-49
AATAC 4024620 5.5503106 40.17913 110-119
AGACG 6378480 5.3158784 101.004364 40-44
ACAGT 5323765 5.263786 35.962925 110-119
GTAGT 6965835 5.2220216 26.746529 80-89
GTGAC 7175195 5.0862765 83.70046 45-49
GAGAC 5877070 4.8979983 141.5029 40-44
ACTCC 5439085 4.852175 122.609406 30-34
CCCCA 5729245 4.8329096 101.5183 60-69
ACCAG 5116960 4.7840095 94.4837 45-49
CTCAG 5901835 4.6932697 35.29463 140-149
GCACA 4806770 4.4940023 34.518898 110-119
GGCCC 8285140 4.4665875 59.315643 60-69
TAGTA 4265210 4.4598374 33.96822 80-89
CACTC 4956570 4.4217267 99.19641 25-29
TGACC 5527080 4.395256 142.99574 45-49
GTGTC 7256360 4.3751554 24.035856 120-129
CGTGT 7221075 4.3538804 23.428457 120-129
CTCCC 5900905 4.2338777 69.782974 30-34
AGTAG 4796280 4.227286 28.003157 70-79
GACCA 4459140 4.168992 106.70542 45-49
ATGCA 4208235 4.1608233 96.47514 25-29
CCCAG 5365265 4.0344124 86.487946 60-69
TGAGG 6065935 3.8330257 68.3189 35-39
AGGAG 5103735 3.7916102 93.80523 35-39
CAGGG 6308250 3.7692385 36.213535 50-59
ACGGT 5292845 3.7519355 95.768425 40-44
TACAC 3377790 3.7465727 27.673985 110-119
GACGG 6220630 3.7168849 106.98032 40-44
CTGAG 5219715 3.7000964 105.15816 35-39
ATGGT 4922425 3.6901548 26.410427 190-199
TGCAC 4583635 3.6450078 115.963 25-29
CCACT 4084480 3.6437402 28.836935 240-247
AGATA 2956755 3.6348426 20.234833 150-159
TGTCC 5346630 3.6164043 20.782028 120-129
GAGGA 4818335 3.5795841 122.83951 35-39
CCCTT 4662345 3.53772 33.79453 20-24
GGGTT 6577065 3.53496 42.98077 50-59
CCTGA 4388630 3.4899356 84.92299 30-34
GCCCC 5750380 3.477721 72.79097 60-69
CCAGG 5106415 3.4228113 41.220646 50-59
GGATG 5358645 3.3860936 69.867 20-24
GCACT 4250845 3.3803656 89.713486 25-29
TGGTG 6233620 3.3503697 18.363047 190-199
CTCTC 4367575 3.314053 13.882606 130-139
AGGCT 4670025 3.3104372 23.709623 30-34
CTTCA 3507150 3.3087525 17.429882 200-209
CAGAT 3345415 3.3077245 18.64508 150-159
TCTTG 4622485 3.3065238 18.38346 170-179
CCGTG 5797715 3.305462 23.540401 120-129
CTTGG 5427125 3.272235 19.134148 20-24
TCAGG 4591525 3.2547917 21.211163 140-149
CCTGG 5698075 3.2486541 29.797176 50-59
TCGCA 4029150 3.2040691 24.18626 100-109
TGTCT 4404200 3.150382 20.292791 180-189
TCTCA 3328995 3.1406758 22.303827 130-139
CGGAT 4408215 3.1248481 79.62746 20-24
TTCCC 4109535 3.118256 50.67404 50-59
CGCAC 4137345 3.1110773 24.694618 100-109
GGTTG 5699830 3.0634747 74.20483 15-19
GGTTC 5020655 3.0271573 48.630886 50-59
GATGC 4264635 3.0230691 97.21016 25-29
ACAGA 2588420 3.0088854 14.375713 140-149
TGGCC 5267600 3.003227 44.002228 60-69
AGGGT 4749575 3.0012262 50.74883 50-59
TCAAA 2122125 2.9266002 59.258644 70-79
AGTCA 2933560 2.9005094 32.569927 70-79
TCTCT 3582280 2.8746033 15.555619 180-189
ACCAC 2726375 2.859485 20.289534 230-239
GTTGG 5316330 2.857356 52.827328 15-19
CCAGT 3564600 2.8346484 37.328087 60-69
GCCCT 4415985 2.8243928 18.23557 20-24
CGGTG 5542905 2.8170238 67.40348 40-44
GCCGT 4897905 2.7924516 22.267279 120-129
GACTC 3503795 2.786295 19.6113 190-199
TTCTT 3267505 2.7728982 21.1702 170-179
GTTCC 4089855 2.766335 55.237534 50-59
GATGG 4325505 2.733259 18.652554 15-19
CGGCC 5043495 2.7189898 12.79766 120-129
AGATG 3003040 2.6467824 17.723806 180-189
TCACA 2370415 2.629214 11.716805 140-149
GCGGA 4315030 2.5782707 87.09504 20-24
TCCAT 2682460 2.5307148 16.50345 70-79
TCCTC 3327355 2.524749 17.294672 130-139
TGTTC 3527540 2.5232954 13.36691 140-149
GGCTG 4947240 2.514294 12.20369 30-34
AGGGA 3335935 2.4782958 9.014567 150-159
TGGAG 3913375 2.472837 15.94493 25-29
ACAGC 2642700 2.4707446 11.193992 120-129
GGCGG 5752150 2.4641194 41.96626 20-24
GAGAT 2758790 2.431508 17.18665 180-189
TTGGG 4519035 2.4288356 79.4277 15-19
CTGGC 4252450 2.4244576 35.71123 60-69
GTTCT 3337720 2.3875148 18.113209 160-169
ACTCT 2529015 2.3859499 13.764299 200-209
AATAA 1383355 2.3720124 9.970984 110-119
CAGCT 2950185 2.346052 12.282666 150-159
TACCA 2105765 2.3356698 17.050035 230-239
CACGG 3469670 2.325707 9.606655 120-129
CATTT 2318185 2.3129025 18.246685 150-159
CTGGA 3260690 2.3114035 14.864211 180-189
TCATT 2315410 2.310134 17.160397 150-159
TTCAT 2269270 2.2640991 16.789335 140-149
TCAGC 2822605 2.244598 14.670916 140-149
TGTAG 2969760 2.2263165 8.058173 210-219
TTCAC 2349420 2.2165146 19.204594 200-209
GTGAA 2490225 2.194804 18.403807 190-199
GATAC 2211265 2.1863523 15.165435 150-159
CAGAG 2617115 2.1811252 9.391059 140-149
TTGTC 3040250 2.1747303 13.719237 170-179
ACTAC 1958155 2.171944 13.189861 230-239
ATTTG 2436840 2.1672752 15.952873 150-159
CTACT 2294530 2.1647298 7.7780476 230-239
TAGTC 2547910 2.1427515 26.870249 70-79
CACTA 1920785 2.1304939 16.047348 240-247
TGGGG 4699855 2.1291947 52.156338 15-19
TCTGC 3137225 2.121986 12.503943 210-219
ATCTC 2229985 2.1038358 9.188377 100-109
CTCGC 3287370 2.1025488 16.334305 100-109
TCTCG 3092965 2.092049 16.825388 100-109
GTCAA 2115480 2.0916464 41.531994 70-79
TTGGA 2779600 2.0837607 16.120754 170-179
GCTGT 3452665 2.0817523 10.360944 140-149
TACAG 2101010 2.0773392 14.113812 160-169
GTTCA 2457045 2.0663357 15.617626 140-149
GAACT 2089875 2.06633 10.87234 160-169
ATATA 1416590 2.0660264 10.910737 110-119
GTCCT 3050380 2.063245 14.691843 130-139
GAATC 2085590 2.0620933 14.705433 190-199
CTCTG 3036380 2.0537755 14.872881 180-189
GTCTC 3031600 2.0505424 12.172418 180-189
CAGGC 3034610 2.0340881 15.777186 140-149
AACCA 1559635 2.0338368 15.74622 70-79
AAAAA 1007855 2.0317626 12.253673 120-129
AGCTC 2552245 2.0296013 12.475966 140-149
ACACA 1556305 2.0294943 13.850098 110-119
AATAT 1386005 2.0214198 13.671927 110-119
ATGTA 1930880 2.0189888 7.4446144 160-169
TTGCA 2394790 2.0139802 14.675872 150-159
TGAAT 1917005 2.0044808 14.156294 190-199
ACACG 2140260 2.0009975 13.502393 120-129
GATAT 1912025 1.9992734 8.221611 180-189
AATCG 2012945 1.9902666 13.624983 190-199
ACGGC 2932295 1.9655068 14.495533 120-129
TTTGC 2728715 1.9518853 12.521731 150-159
TGGAA 2196365 1.9358053 9.439356 170-179
CCTTC 2550665 1.9354079 14.351908 200-209
GAGTC 2695940 1.9110693 12.655318 210-219
GGGGC 4447995 1.9054424 47.551456 15-19
CAGCC 2523485 1.8975351 10.125399 120-129
TCGGC 3323735 1.8949674 8.000369 130-139
TACTA 1611420 1.8902078 10.420617 230-239
ATATG 1806570 1.8890064 8.458617 180-189
CATAG 1894650 1.8733041 8.76885 210-219
GGGCG 4334435 1.8567954 59.12402 20-24
CTGTT 2540030 1.8169167 13.092378 140-149
AGTAT 1709905 1.7879304 12.387144 220-229
CCAGA 1901620 1.7778852 25.677607 60-69
GACGT 2490445 1.7654002 9.881257 170-179
GCAGA 2109735 1.7582706 14.8952 150-159
CTCCA 1959510 1.7480673 8.07802 150-159
GTCAC 2197925 1.7478385 8.604947 130-139
ATTGT 1963440 1.746243 7.8421073 170-179
TGCAG 2461760 1.7450664 13.91624 150-159
CGTAG 2460235 1.7439852 9.993776 210-219
AGAAC 1500110 1.7437893 11.494133 160-169
GTTGT 2732070 1.7420708 7.051754 210-219
AGTCT 2019270 1.6981738 13.711576 210-219
CACCA 1604050 1.6823646 13.921986 230-239
ATAGT 1606295 1.6795926 7.8229823 220-229
GGTCA 2369305 1.6795276 7.5079584 130-139
TTCAG 1989995 1.6735541 9.474949 150-159
CCTTG 2460195 1.66405 21.832296 20-24
TCAGA 1679115 1.6601977 10.377725 130-139
CAAAA 1021515 1.656261 21.397299 70-79
TGGTT 2596770 1.6557984 18.21056 10-14
GTCTG 2732080 1.6472825 9.391703 210-219
TGACT 1938965 1.6306386 11.738099 190-199
AACTG 1642590 1.624084 10.739126 160-169
CAAAG 1394705 1.6212622 29.913052 70-79
GAACC 1720385 1.608443 13.054812 70-79
GAGAA 1550375 1.6065174 10.48155 160-169
TCTGG 2658425 1.6028728 12.193054 180-189
TCCCA 1786585 1.5938019 11.100334 240-247
CTGTG 2630240 1.585879 5.520765 210-219
AATAG 1281905 1.5758905 10.089886 240-247
GCTCC 2463735 1.575765 6.5462966 150-159
TGTGC 2603080 1.5695031 8.508636 220-229
TGCTC 2311375 1.5633898 5.3638754 150-159
CTGGT 2586780 1.5596752 7.049361 160-169
GTCCC 2435820 1.5579113 19.238617 50-59
ACTGA 1569270 1.55159 8.787568 180-189
GGCCG 3227060 1.550818 13.175507 120-129
TTGGT 2426200 1.5470365 23.331089 25-29
TTGTA 1735480 1.5435002 9.134154 210-219
TAGTT 1730615 1.5391732 9.95954 220-229
GTGCT 2542130 1.5327538 8.302829 220-229
ACCAT 1377310 1.5276829 11.393827 45-49
GCTCA 1919200 1.52619 11.153057 140-149
ATAGA 1239890 1.5242401 10.3391075 240-247
GGACG 2533325 1.5136853 7.3156047 170-179
GCTCT 2203805 1.4906304 10.224475 130-139
CCTCG 2322930 1.4857086 9.81205 130-139
CCACC 1739145 1.4670573 10.043634 230-239
CCCAC 1723290 1.4536828 10.64927 240-247
AAATA 843495 1.4463248 5.6286144 9
AATTG 1370360 1.4328915 9.606762 170-179
CAGAC 1521900 1.4228729 14.726738 60-69
GCTGA 2005970 1.4219706 20.17933 30-34
TATAC 1211120 1.4206529 6.8823285 110-119
CATGT 1685930 1.4178401 7.209633 70-79
CGACT 1769715 1.4073163 8.696608 190-199
CTGCT 2043940 1.3824996 5.79272 90-99
TGGAC 1949985 1.3822848 10.49705 170-179
CACTG 1735135 1.3798175 7.4537034 230-239
TGATA 1317385 1.3774993 7.717677 180-189
TCGAC 1730825 1.3763903 9.356471 190-199
CTCTT 1707905 1.3705096 8.223875 200-209
AGATC 1384515 1.3689167 7.205026 130-139
CCTCA 1526450 1.361737 7.4857335 130-139
GCGTA 1908635 1.3529729 10.577808 210-219
GAATT 1272910 1.3309948 10.488219 170-179
GGGAG 2496180 1.3295306 6.452863 150-159
ACGGA 1589235 1.3244816 9.666683 200-209
GTAGG 2084765 1.3173497 5.226492 160-169
CTTGA 1564905 1.3160602 8.443088 200-209
GAGGG 2463450 1.3120978 8.1063385 200-209
CTGAT 1550675 1.3040929 7.3908195 180-189
GGAAT 1476310 1.3011718 8.8184595 170-179
CCATC 1458275 1.3009185 11.317569 240-247
ACTTC 1377625 1.2996935 6.887841 220-229
ATCGG 1832755 1.2991838 8.028061 190-199
CCATG 1623950 1.2914008 7.3156853 70-79
CTCGA 1623275 1.2908641 8.382872 190-199
GTGGA 2041025 1.2897108 20.31377 25-29
TATGG 1710480 1.2822819 6.0594854 180-189
AGCCG 1912170 1.2817205 9.000023 120-129
GTGTT 1996130 1.2728077 7.80215 160-169
AAAGT 1032870 1.2697431 21.887037 70-79
AAGTA 1031845 1.2684832 15.991011 70-79
TCACG 1581275 1.2574649 9.932932 200-209
ACGTG 1768300 1.2534937 9.46256 170-179
GGCTC 2193005 1.2503021 8.412015 130-139
GGAGT 1976540 1.248963 7.2283735 210-219
ACCGT 1569700 1.24826 17.503117 45-49
TCCAC 1395535 1.2449485 9.695599 240-247
ATAAC 898130 1.2386016 7.9128647 80-89
ACTAT 1052180 1.234215 6.452717 230-239
GATCT 1460010 1.2278451 5.626385 130-139
ATAAA 705855 1.2103162 6.5101533 7
GTCCA 1521385 1.209839 15.51348 70-79
GGAGG 2242385 1.1943527 15.50847 30-34
ACTCG 1501715 1.1941968 8.5648365 190-199
TTGGC 1963490 1.1838682 7.865278 60-69
GCATA 1193985 1.180533 8.519802 210-219
ACTGG 1654550 1.1728598 7.1659894 160-169
AGAGC 1405325 1.1712095 6.91166 140-149
GTGGT 2177270 1.1702125 9.923176 50-59
TTGAG 1560465 1.1698214 8.097703 200-209
TGGTC 1939115 1.1691716 11.3292265 50-59
TATCA 994080 1.1660633 7.0215063 70-79
AGCTT 1380640 1.1610961 6.119168 150-159
TTCCA 1228540 1.1590422 7.807153 230-239
ATATC 974015 1.1425269 7.4664917 70-79
AGTTC 1343585 1.1299335 5.470279 160-169
TGCCC 1766415 1.1297705 6.2628627 60-69
ACTGT 1342105 1.1286888 5.8460093 230-239
ATACC 1014715 1.1255003 6.9933825 70-79
GAAGA 1084630 1.1239069 18.43156 35-39
CAATA 809585 1.11649 6.5977793 100-109
AGGTT 1487380 1.1150322 22.95127 10-14
GTCCG 1948610 1.1109647 5.06925 130-139
TCTGT 1550070 1.1087854 6.857238 210-219
CCATA 993805 1.1023073 9.723805 70-79
CTGAA 1112275 1.0997438 14.516054 35-39
AAAAG 758410 1.0961394 14.143544 70-79
CGGCT 1915165 1.0918967 7.9279027 130-139
CTCGG 1908485 1.0880882 8.529167 130-139
TCTAC 1152615 1.0874122 7.247164 180-189
GTCTA 1292730 1.0871654 7.3216853 180-189
GCTTC 1563475 1.0575179 5.479403 150-159
TACTG 1249030 1.0504142 6.4286394 180-189
GAGCT 1479965 1.0491017 6.201566 140-149
TATGT 1172725 1.0429974 7.8270054 220-229
ATCCA 932600 1.03442 15.764354 240-247
CTTCT 1280795 1.0277749 5.6449227 210-219
CTGCG 1802190 1.027486 6.9978924 210-219
AGAGA 991545 1.027451 12.709372 35-39
CTGCC 1602090 1.024671 6.078983 200-209
AGGGG 1919705 1.022485 5.1039386 60-69
ATGGG 1611325 1.018186 29.18525 15-19
CATGG 1433595 1.0162315 5.5132895 190-199
AACTT 865745 1.0155253 6.248988 200-209
ATACG 1027060 1.0154889 5.273869 120-129
GCGGT 1990370 1.011549 5.747063 130-139
GGGGT 2226785 1.0088094 7.684015 10-14
CTACC 1125755 1.0042794 8.914054 230-239
CGAAC 1072820 1.0030137 13.634222 70-79
AAAAT 584170 1.0016651 5.2374496 8
ACGTC 1257810 1.0002382 11.08805 70-79
AGAAA 691035 0.99876165 5.0953555 6
CAGCG 1473705 0.987819 6.521537 160-169
AAGAG 952895 0.9874014 13.699721 35-39
TAGAT 941125 0.9840699 8.000805 240-247
TATAA 668450 0.9749013 6.6895866 240-247
TGCGT 1604670 0.967521 8.448105 210-219
AGCTG 1344970 0.953408 5.049792 90-99
TGCTA 1128930 0.9494121 5.1134763 220-229
ACAAT 687675 0.9483654 7.6529984 100-109
TAGGC 1330055 0.9428352 5.9360337 160-169
TCGAA 945765 0.9351098 17.262478 70-79
CGGAG 1561175 0.9328166 6.801261 210-219
CCATT 978890 0.92351466 5.8112473 50-59
GAGTT 1228360 0.9208548 5.2232513 160-169
AGTTT 1034270 0.9198584 5.107359 220-229
TGTAA 878995 0.9191049 7.0078497 230-239
TGAAC 921085 0.91070783 6.7698445 190-199
TTCTG 1272600 0.91030735 5.6780906 210-219
ACCCA 863580 0.9057426 13.246528 240-247
CGTGG 1769630 0.8993641 6.8672647 50-59
TGCAT 1067045 0.89736784 6.480875 210-219
ACTAA 649200 0.8953048 13.213568 240-247
CCCAA 844645 0.88588315 9.930388 240-247
AGCGT 1232435 0.8736354 6.705108 160-169
TGAAG 988610 0.87132895 9.621513 35-39
CATCA 775895 0.8606062 5.952465 230-239
GTATG 1137685 0.8528793 5.4150705 220-229
CCAGC 1129930 0.8496511 5.701619 80-89
CGTCC 1317160 0.8424342 9.202856 70-79
ATCAA 607375 0.83762443 12.807159 70-79
GAGGC 1399365 0.83613366 13.151893 30-34
CCAAT 752300 0.83443516 10.791007 240-247
CGGTC 1456695 0.83050823 5.442103 130-139
GGTGG 1828310 0.8282867 20.175022 25-29
TAACC 728770 0.80833626 6.330316 80-89
CTTCC 1044810 0.7927868 5.4877515 230-239
GGGCC 1639770 0.78801906 15.344939 15-19
AACCG 842490 0.7876708 5.9239264 190-199
GACCG 1159155 0.77697736 16.214191 45-49
AAAGG 738755 0.7655068 8.763571 10-14
TGAGA 863835 0.76135623 5.7637796 240-247
AAAGA 522985 0.7558768 5.996767 8
GTCGA 1052380 0.746 12.310515 70-79
AGCCA 792975 0.7413776 5.961021 80-89
TGGGC 1449135 0.7364818 16.658203 15-19
GGTCC 1273695 0.7261741 11.537519 50-59
CTAAT 609595 0.7150596 10.151808 240-247
ATGAT 682110 0.7132357 7.179491 240-247
GTAGC 988675 0.7008414 5.362519 80-89
AATCC 623725 0.6918225 9.527997 240-247
AAGGT 783295 0.69037086 9.754639 10-14
GGAAG 928310 0.6896498 6.112742 30-34
AGACC 733310 0.685595 5.0015745 240-247
CATAC 613030 0.67995983 8.312137 70-79
AAAGC 581310 0.6757385 9.627414 70-79
TGTTA 756515 0.67282885 5.1964526 230-239
CATTG 791050 0.6652604 5.2693267 50-59
AGGAT 732560 0.6456546 5.7989845 10-14
TAGGT 857655 0.64295137 5.4029126 10-14
TATGA 599815 0.6271854 5.7678742 240-247
ATCCC 700240 0.6246799 5.0124917 240-247
GAGGT 984620 0.6221751 6.612232 10-14
ATAGG 694615 0.61221117 5.2883883 10-14
AGAAG 588380 0.60968655 5.089206 9
CAATC 547465 0.6072365 9.270049 240-247
GGGTC 1175325 0.5973255 5.936055 70-79
CGATG 838245 0.5942062 13.010654 15-19
CCGTA 745860 0.5931243 5.0494046 80-89
GAAAA 401830 0.58077 5.9276805 8
AATGG 658450 0.5803365 6.4266996 10-14
AAATG 455695 0.56020176 5.165098 9
TAAAA 325890 0.55879736 6.4976344 8
CATCC 612350 0.54627377 7.497089 240-247
AAGAA 362835 0.52441 5.2728825 9
AAGCA 447845 0.5205934 6.440273 70-79
GAAGG 698295 0.5187696 5.178466 10-14
AAGGG 696980 0.51779264 6.294377 10-14
GGTCG 990195 0.50323844 6.02425 70-79
TGATC 583225 0.49048293 5.823098 240-247
GATCC 586625 0.46649712 5.8732905 240-247
ATAAG 375345 0.4614247 5.586709 9
ACGAT 456980 0.45183152 5.671256 10-14
CGGTT 741320 0.446972 7.962665 10-14
TAAGG 499980 0.4406662 5.7541184 10-14
TAAAG 330130 0.40584034 5.107806 9
AAGCT 396050 0.391588 7.7433925 30-34
TGGGT 703865 0.37830478 5.0264583 10-14