FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_18_SL248347.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_18_SL248347.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5175723
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 5859700 8.8582945 62.18613 110-119
ATACA 5359005 7.660467 37.804817 110-119
CACAG 7804130 7.5622582 42.420025 110-119
TCCCT 9245365 7.269024 107.262566 30-34
CAGTA 6670415 6.835706 39.68922 110-119
AGTAA 5106325 6.506987 48.101654 110-119
GGAGA 8118200 6.2515464 93.50236 35-39
CCCTG 9253150 6.1325116 70.04874 30-34
GTAAT 5557335 6.0228314 40.07806 110-119
GGTGA 9038560 5.9195633 102.084724 45-49
AATAC 3885905 5.5547338 40.03371 110-119
AGACG 6159150 5.3204474 100.85786 40-44
ACAGT 5147700 5.275259 36.023247 110-119
GTAGT 6729715 5.228659 26.80705 80-89
GTGAC 6928685 5.09027 84.10812 45-49
GAGAC 5649505 4.880202 140.98769 40-44
ACTCC 5247450 4.8510704 122.64591 30-34
CCCCA 5525885 4.8304496 101.472595 60-69
ACCAG 4939760 4.786663 94.721924 45-49
CTCAG 5704740 4.7013817 35.336235 140-149
GCACA 4646490 4.502482 34.634186 110-119
GGCCC 7987435 4.4622393 59.257008 60-69
TAGTA 4107140 4.4511647 33.95619 80-89
CACTC 4783020 4.421723 98.98409 25-29
TGACC 5338495 4.3995523 143.37964 45-49
GTGTC 7021165 4.386942 24.059752 120-129
CGTGT 6981745 4.3623123 23.427341 120-129
AGTAG 4634545 4.2338753 28.005238 70-79
CTCCC 5692000 4.2316847 69.874695 30-34
GACCA 4305660 4.1722155 106.89295 45-49
ATGCA 4052560 4.152982 96.608315 25-29
CCCAG 5174665 4.0324435 86.43479 60-69
TGAGG 5850335 3.8315206 68.480644 35-39
AGGAG 4906135 3.7780461 93.73929 35-39
CAGGG 6092975 3.7732553 36.238113 50-59
TACAC 3264415 3.7526283 27.540108 110-119
ACGGT 5102965 3.7489755 95.29762 40-44
GACGG 6000410 3.715932 106.48165 40-44
CTGAG 5034815 3.698908 105.13234 35-39
ATGGT 4753025 3.6928675 26.51381 190-199
TGCAC 4417825 3.6408112 115.829285 25-29
CCACT 3937900 3.6404407 28.865824 240-247
AGATA 2855385 3.6386154 20.086496 150-159
TGTCC 5164450 3.6197321 20.743427 120-129
GAGGA 4636550 3.570448 122.92061 35-39
CCCTT 4499895 3.5379717 33.880024 20-24
GGGTT 6345110 3.5342085 42.944916 50-59
CCTGA 4226675 3.4832807 84.78798 30-34
GCCCC 5549030 3.4774613 72.748795 60-69
CCAGG 4933355 3.4271162 41.254185 50-59
GGATG 5161660 3.380491 69.66274 20-24
GCACT 4095145 3.3748848 89.51141 25-29
TGGTG 6029030 3.3581536 18.401924 190-199
CTCTC 4220985 3.3186834 13.844098 130-139
AGGCT 4515975 3.317734 23.866755 30-34
TCTTG 4472625 3.3152661 18.382046 170-179
CTTCA 3390105 3.3144083 17.314472 200-209
CCGTG 5608260 3.3134258 23.535933 120-129
CAGAT 3228340 3.3083377 18.53437 150-159
CTTGG 5245060 3.2772017 19.195219 20-24
TCAGG 4444990 3.2655838 21.247992 140-149
CCTGG 5496205 3.2472222 29.801563 50-59
TCGCA 3893345 3.2085776 24.172985 100-109
TGTCT 4257045 3.155471 20.365131 180-189
TCTCA 3210335 3.1386523 22.222654 130-139
CGGAT 4245555 3.1190655 79.39987 20-24
CGCAC 3998640 3.1160073 24.636631 100-109
TTCCC 3960390 3.1137943 50.642628 50-59
GGTTG 5500785 3.0639224 74.2416 15-19
GGTTC 4844090 3.026669 48.591988 50-59
GATGC 4106290 3.0167522 97.25877 25-29
ACAGA 2501355 3.014 14.443997 140-149
AGGGT 4580115 2.9996243 50.756775 50-59
TGGCC 5074775 2.9982367 43.94263 60-69
TCAAA 2047550 2.9268844 59.210766 70-79
AGTCA 2832265 2.902448 32.631813 70-79
TCTCT 3456275 2.8738484 15.481878 180-189
GTTGG 5131950 2.8584826 52.886494 15-19
ACCAC 2624810 2.8531494 20.18802 230-239
CCAGT 3434450 2.8303933 37.257534 60-69
GCCCT 4258030 2.8220034 18.284855 20-24
CGGTG 5348715 2.8170788 67.05932 40-44
GACTC 3400120 2.8021019 19.754168 190-199
GCCGT 4735530 2.7978072 22.25347 120-129
TTCTT 3153290 2.7728286 21.163103 170-179
GTTCC 3943560 2.7640176 55.13502 50-59
GATGG 4167830 2.7296088 18.676811 15-19
CGGCC 4874610 2.723237 12.79354 120-129
AGATG 2892835 2.642741 17.634544 180-189
TCACA 2294575 2.6377428 11.85029 140-149
GCGGA 4156455 2.5740082 86.92816 20-24
TCCAT 2588215 2.5304236 16.542791 70-79
TCCTC 3215815 2.5283842 17.318758 130-139
TGTTC 3400035 2.5202253 13.314946 140-149
GGCTG 4778780 2.5169034 12.298994 30-34
AGGGA 3235410 2.4914782 9.058352 150-159
TGGAG 3772005 2.4703739 16.079674 25-29
GGCGG 5553605 2.4655924 41.98371 20-24
ACAGC 2541345 2.4625814 11.219465 120-129
GAGAT 2657390 2.427651 17.132267 180-189
TTGGG 4357160 2.426926 79.3732 15-19
CTGGC 4098905 2.4216814 35.673637 60-69
ACTCT 2452070 2.3973184 13.86101 200-209
GTTCT 3219555 2.3864472 18.02989 160-169
AATAA 1330080 2.3642302 9.946076 110-119
CAGCT 2850365 2.3490386 12.382299 150-159
TACCA 2026965 2.33011 16.97596 230-239
CACGG 3347475 2.325433 9.636131 120-129
CATTT 2234745 2.310598 18.198952 150-159
CTGGA 3143700 2.3095698 14.729422 180-189
TCATT 2228965 2.3046217 17.165026 150-159
TTCAT 2187275 2.2615168 16.646242 140-149
TCAGC 2720815 2.242274 14.62576 140-149
TGTAG 2878090 2.236135 8.052439 210-219
TTCAC 2264935 2.214362 19.135962 200-209
GTGAA 2402330 2.1946416 18.330608 190-199
GATAC 2133650 2.1865215 15.042126 150-159
CAGAG 2528085 2.1838312 9.451653 140-149
CTACT 2220260 2.1706843 7.795795 230-239
TTGTC 2926285 2.1690648 13.611702 170-179
ACTAC 1886625 2.1687815 13.122805 230-239
ATTTG 2344620 2.1610723 15.89872 150-159
TAGTC 2458165 2.1424184 26.884354 70-79
TGGGG 4529205 2.1265316 52.10706 15-19
TCTGC 3027585 2.122016 12.435247 210-219
CACTA 1844330 2.120161 16.034529 240-247
ATCTC 2160325 2.112088 9.222969 100-109
CTCGC 3183860 2.1100984 16.352135 100-109
TCTCG 3001495 2.1037297 16.908157 100-109
TTGGA 2687695 2.0882072 16.128458 170-179
GTCAA 2035535 2.0859752 41.437828 70-79
GAACT 2029585 2.0798779 10.919219 160-169
GCTGT 3325925 2.078094 10.298697 140-149
AAAAA 991715 2.0726988 12.62694 120-129
TACAG 2017895 2.067898 14.025351 160-169
ATATA 1367100 2.0666883 10.941791 110-119
GTCCT 2945510 2.06449 14.698363 130-139
GTTCA 2367550 2.063443 15.534822 140-149
GAATC 2009500 2.059295 14.636131 190-199
CTCTG 2923840 2.0493019 14.76717 180-189
GTCTC 2920440 2.0469186 12.113207 180-189
CAGGC 2934145 2.0382998 15.791336 140-149
ACACA 1504285 2.0332818 13.906626 110-119
AGCTC 2466825 2.0329561 12.478015 140-149
AACCA 1501240 2.0291662 15.732132 70-79
ATGTA 1867445 2.023867 7.414011 160-169
AATAT 1337000 2.021185 13.700747 110-119
GATAT 1858260 2.0139127 8.308089 180-189
TTGCA 2307555 2.011154 14.578183 150-159
ACACG 2069805 2.0056558 13.542043 120-129
TGAAT 1849475 2.0043917 14.043519 190-199
AATCG 1942455 1.9905885 13.582393 190-199
ACGGC 2835280 1.96962 14.520081 120-129
TTTGC 2632990 1.9516643 12.446731 150-159
CCTTC 2461680 1.9354571 14.300884 200-209
TGGAA 2118340 1.9352034 9.466443 170-179
GAGTC 2595810 1.9070537 12.638121 210-219
GGGGC 4286550 1.9030675 47.455166 15-19
ATATG 1752270 1.8990448 8.526437 180-189
CAGCC 2431240 1.8945843 10.108189 120-129
TCGGC 3201390 1.8914189 7.9541183 130-139
TACTA 1549200 1.8833944 10.386107 230-239
CATAG 1819695 1.8647865 8.684399 210-219
GGGCG 4174390 1.8532726 59.1123 20-24
CTGTT 2446530 1.8134537 13.006874 140-149
AGTAT 1644965 1.7827514 12.35101 220-229
CCAGA 1835180 1.7783024 25.68332 60-69
GACGT 2416535 1.7753464 9.832412 170-179
GTCAC 2134205 1.7588378 8.684532 140-149
AGAAC 1458490 1.757403 11.586485 160-169
GCAGA 2032860 1.7560416 14.755269 150-159
CTCCA 1895420 1.752245 8.129381 150-159
ATTGT 1895450 1.7470654 7.84511 170-179
GTTGT 2639905 1.7443941 7.081812 210-219
TGCAG 2373350 1.7436199 13.841244 150-159
CGTAG 2370060 1.7412028 9.916869 210-219
AGTCT 1945215 1.6953559 13.644554 210-219
GGTCA 2294340 1.6855739 7.5572214 130-139
CACCA 1547255 1.6818547 13.903996 230-239
TTCAG 1926810 1.6793149 9.50428 150-159
ATAGT 1536975 1.6657159 7.77964 220-229
TCAGA 1623025 1.6632432 10.391128 130-139
CCTTG 2371050 1.6618546 21.909077 20-24
TGGTT 2512580 1.6602604 18.125792 10-14
CAAAA 984820 1.6552563 21.444248 70-79
GTCTG 2636360 1.6472421 9.34025 210-219
TGACT 1877635 1.6364564 11.848736 190-199
AACTG 1593090 1.6325665 10.775565 160-169
CAAAG 1345380 1.6211114 29.836622 70-79
GAGAA 1505150 1.6167692 10.567664 160-169
GAACC 1660910 1.6094337 13.077694 70-79
TCTGG 2559610 1.5992874 12.115507 180-189
TCCCA 1722860 1.5927197 11.063785 240-247
GCTCC 2386950 1.5819476 6.56569 150-159
CTGTG 2525830 1.578181 5.500856 210-219
AATAG 1236905 1.5761871 10.063138 240-247
TGCTC 2235025 1.5665154 5.384953 150-159
TGTGC 2502070 1.5633355 8.436305 220-229
ACTGA 1523780 1.5615388 8.835122 180-189
CTGGT 2498095 1.5608519 7.059834 160-169
GTCCC 2351090 1.5581814 19.289991 50-59
GGCCG 3119450 1.5535454 13.200505 120-129
TTGGT 2346245 1.5503496 23.341383 25-29
TTGTA 1680710 1.5491362 9.137573 210-219
TAGTT 1672655 1.5417119 10.055783 220-229
GTGCT 2452995 1.5326725 8.272048 220-229
ATAGA 1201090 1.5305483 10.3903055 240-247
GCTCA 1854355 1.5282083 11.152782 140-149
ACCAT 1326145 1.5244782 11.362221 45-49
GGACG 2450995 1.5178511 7.301522 170-179
GCTCT 2117860 1.4843953 10.157752 130-139
CCTCG 2237185 1.4826909 9.780193 130-139
CCACC 1677830 1.4666742 10.029281 230-239
CCCAC 1667345 1.4575087 10.633946 240-247
AAATA 809510 1.438912 5.490134 9
AATTG 1321845 1.4325662 9.612396 170-179
CAGAC 1469400 1.4238591 14.775017 60-69
GCTGA 1938050 1.4238197 20.290676 30-34
TATAC 1167790 1.4197063 6.8955655 110-119
CGACT 1722245 1.4193339 8.777625 190-199
CATGT 1626640 1.4177011 7.2620525 70-79
TGATA 1281205 1.388522 7.790721 180-189
TCGAC 1682485 1.386567 9.415943 190-199
CTGCT 1976895 1.3855938 5.8315134 90-99
TGGAC 1885490 1.3852056 10.512435 170-179
CACTG 1673200 1.3789151 7.4186482 230-239
CTCTT 1653415 1.3747935 8.270285 200-209
AGATC 1337540 1.370684 7.202924 130-139
CCTCA 1480605 1.3687636 7.532364 130-139
GCGTA 1839535 1.3514441 10.528535 210-219
GGGAG 2421245 1.3366754 6.475269 150-159
GAATT 1225265 1.3278964 10.487332 170-179
GAGGG 2394745 1.3220459 8.191305 200-209
ACGGA 1530215 1.3218428 9.677007 200-209
CTTGA 1516470 1.3216822 8.464485 200-209
GTAGG 2010740 1.3168805 5.2828245 160-169
CTGAT 1502290 1.3093237 7.4268756 180-189
CTCGA 1579740 1.301893 8.448925 190-199
ACTTC 1330440 1.300733 6.925235 220-229
CCATC 1406010 1.2998035 11.312501 240-247
GGAAT 1422550 1.2995663 8.791968 170-179
ATCGG 1767540 1.2985518 7.970736 190-199
CCATG 1565000 1.2897455 7.3540673 70-79
TATGG 1659210 1.2891248 6.155236 180-189
GTGGA 1966060 1.2876185 20.434181 25-29
AGCCG 1843210 1.2804462 8.975939 120-129
GTGTT 1927085 1.2733775 7.7779717 160-169
AAAGT 994045 1.266711 21.861626 70-79
AAGTA 993785 1.2663796 16.01038 70-79
ACGTG 1709580 1.2559706 9.420291 170-179
TCACG 1520790 1.2533112 9.950147 200-209
ACCGT 1519705 1.252417 17.526983 45-49
GGCTC 2110325 1.2468047 8.351194 130-139
GGAGT 1900655 1.2447832 7.229343 210-219
ATAAC 869450 1.2428414 7.916869 80-89
TCCAC 1344190 1.2426533 9.683614 240-247
ACTAT 1013940 1.2326677 6.3977985 230-239
GATCT 1412980 1.2314854 5.6105957 130-139
GTCCA 1466440 1.2085203 15.506794 70-79
ATAAA 677920 1.2050096 6.3384323 7
ACTCG 1458010 1.201573 8.612128 190-199
GGAGG 2169105 1.1974787 15.538721 30-34
TTGGC 1893350 1.1829969 7.835981 60-69
ACTGG 1601080 1.1762593 7.1832485 160-169
GCATA 1147010 1.1754327 8.451839 210-219
AGAGC 1359130 1.1740549 6.9359336 140-149
GTGGT 2106900 1.1735375 9.961156 50-59
TTGAG 1508680 1.1721704 8.120024 200-209
TGGTC 1869180 1.1678951 11.352885 50-59
TATCA 957165 1.1636451 7.021272 70-79
AGCTT 1333590 1.1622928 6.1863036 150-159
TTCCA 1182970 1.1565558 7.755105 230-239
ATATC 943865 1.1474762 7.4710345 70-79
TGCCC 1707400 1.1315768 6.3016496 60-69
AGTTC 1295680 1.1292523 5.409787 160-169
ATACC 978095 1.1243751 6.975318 70-79
GAAGA 1044645 1.122114 18.43936 35-39
ACTGT 1284860 1.1198221 5.821632 230-239
AGGTT 1434190 1.1142954 22.917444 10-14
GTCCG 1885975 1.1142561 5.130756 130-139
CAATA 778980 1.1135184 6.6421895 100-109
TCTGT 1491960 1.1058929 6.8355193 210-219
CCATA 956720 1.0998034 9.709521 70-79
AAAAG 733475 1.098991 14.147917 70-79
CTGAA 1069860 1.0963709 14.501789 35-39
TCTAC 1117215 1.0922691 7.285919 180-189
GTCTA 1251675 1.0908997 7.3869433 180-189
CGGCT 1840295 1.087268 7.847727 130-139
CTCGG 1835455 1.0844084 8.480987 130-139
GCTTC 1516245 1.062727 5.505255 150-159
TACTG 1210460 1.0549787 6.461606 180-189
GAGCT 1429920 1.0505139 6.2156405 140-149
TATGT 1130010 1.0415477 7.8094993 220-229
ATCCA 897490 1.0317152 15.661538 240-247
CTTCT 1238405 1.0297179 5.6735935 210-219
AGAGA 955990 1.0268846 12.641447 35-39
CTGCG 1736490 1.0259386 6.9504647 210-219
AGGGG 1857630 1.0255255 5.094511 60-69
CTGCC 1538645 1.0197347 6.0776386 200-209
AACTT 837745 1.0184639 6.2611356 200-209
GCGGT 1933235 1.0182025 5.8054485 130-139
ATGGG 1553355 1.0173284 29.285952 15-19
CATGG 1383640 1.0165135 5.539169 190-199
ATACG 990585 1.0151315 5.281452 120-129
GGGGT 2155490 1.0120358 7.696953 10-14
ACGTC 1219295 1.0048436 11.106952 70-79
CGAAC 1033165 1.0011443 13.648624 70-79
CTACC 1082335 1.000578 8.844524 230-239
AAAAT 560190 0.9957433 5.2956524 8
TAGAT 911120 0.9874377 8.020599 240-247
AAGAG 918180 0.9862706 13.680904 35-39
CAGCG 1414270 0.9824689 6.446381 160-169
TATAA 646250 0.9769565 6.6719723 240-247
TGCGT 1543425 0.96435803 8.391738 210-219
AGCTG 1292870 0.9498278 5.0438776 90-99
TGCTA 1086605 0.9470326 5.0560837 220-229
ACAAT 661720 0.9459002 7.6604967 100-109
TCCCC 1269305 0.9436575 5.0043387 90-99
TAGGC 1283025 0.942595 5.998457 160-169
TCGAA 914690 0.9373559 17.329441 70-79
CGGAG 1501260 0.9296998 6.8048854 210-219
CCATT 946300 0.92517036 5.8110414 50-59
TGTAA 853220 0.9246879 7.088769 230-239
GAGTT 1182320 0.91860473 5.178395 160-169
AGTTT 991670 0.91403747 5.1034355 220-229
ACCCA 838545 0.9114922 13.248252 240-247
TTCTG 1228690 0.910748 5.6665115 210-219
TGAAC 888235 0.9102453 6.7546287 190-199
CGTGG 1710345 0.9008101 6.895746 50-59
TGCAT 1025855 0.8940859 6.441382 210-219
ACTAA 621840 0.8888934 13.070443 240-247
CCCAA 816170 0.8871707 9.988149 240-247
TGAAG 950200 0.8680525 9.556896 35-39
AGCGT 1179380 0.86645055 6.640291 160-169
CATCA 745895 0.8574482 5.9388995 230-239
CCAGC 1089610 0.84909666 5.702573 80-89
GTATG 1091970 0.84840715 5.3886333 220-229
CGTCC 1274580 0.844726 9.229227 70-79
ATCAA 589065 0.8420431 12.8920355 70-79
GAGGC 1353375 0.83811766 13.199973 30-34
CGGTC 1417675 0.8375791 5.503561 130-139
CCAAT 727255 0.83602047 10.900195 240-247
GGTGG 1758895 0.8258283 20.246943 25-29
TAACC 701820 0.80678153 6.2732697 80-89
CTTCC 1007130 0.79184014 5.473174 230-239
GGGCC 1580085 0.7869123 15.406466 15-19
AACCG 811870 0.7867079 5.9202104 190-199
GACCG 1120460 0.7783642 16.212027 45-49
AAAGG 711345 0.76409715 8.753323 10-14
TGAGA 834460 0.76231855 5.771608 240-247
AAAGA 504750 0.7562844 5.7966447 8
GTCGA 1016860 0.7470526 12.317711 70-79
AGCCA 761040 0.7374532 5.9741187 80-89
TGGGC 1395725 0.735105 16.741335 15-19
GGTCC 1229210 0.7262317 11.574036 50-59
ATGAT 659685 0.7149419 7.2822585 240-247
CTAAT 584615 0.7107285 10.0654545 240-247
GTAGC 953335 0.70038295 5.320297 80-89
AATCC 602165 0.6922225 9.570133 240-247
GGAAG 897800 0.6913649 6.0671563 30-34
AAGGT 754725 0.68947685 9.753983 10-14
CATAC 589920 0.6781462 8.310761 70-79
AAAGC 561460 0.6765295 9.604932 70-79
TGTTA 728395 0.6713729 5.154486 230-239
CATTG 762110 0.66421837 5.269959 50-59
AGGAT 712860 0.65123117 5.8028584 10-14
TAGGT 822925 0.6393724 5.384495 10-14
TATGA 577440 0.6258079 5.8167143 240-247
GAGGT 949625 0.62193155 6.590743 10-14
ATAGG 670515 0.61254704 5.269767 10-14
CAATC 529395 0.6085693 9.306367 240-247
AGAAG 566195 0.608183 5.128079 9
GGGTC 1134415 0.5974773 5.9484243 70-79
CGATG 809100 0.59441835 12.974036 15-19
CCGTA 718915 0.5924711 5.0086403 80-89
GAAAA 390750 0.5854742 6.240886 8
AATGG 633665 0.5788828 6.4477654 10-14
TAAAA 315940 0.5615865 6.42406 8
AAATG 438785 0.5591435 5.282372 9
CATCC 587480 0.5431032 7.4752183 240-247
AAGCA 432230 0.5208142 6.4233375 70-79
AAGAA 346875 0.51973474 5.3460317 9
GAAGG 670115 0.5160326 5.119919 10-14
AAGGG 669930 0.5158901 6.2771106 10-14
GGTCG 956865 0.5039648 6.049926 70-79
TGATC 559395 0.48754174 5.7676415 240-247
GATCC 561510 0.46275076 5.7745924 240-247
ATAAG 361395 0.46052545 5.597669 9
ACGAT 440675 0.45159486 5.6931305 10-14
CGGTT 712530 0.44520077 7.985033 10-14
TAAGG 480985 0.43940246 5.7703185 10-14
TAAAG 316010 0.40269136 5.0615115 9
AAGCT 381000 0.39044112 7.7089705 30-34
TGGGT 683225 0.38055447 5.028983 10-14