FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_7_SL248353.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_7_SL248353.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5934254
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 6659890 8.8561325 61.456944 110-119
ATACA 6133375 7.668094 37.546215 110-119
CACAG 8996015 7.5542426 41.56361 110-119
TCCCT 10424955 7.0851345 102.598015 30-34
CAGTA 7448265 6.6525016 38.928955 110-119
AGTAA 5841430 6.5255446 47.52276 110-119
GGAGA 9326500 6.252861 94.57109 35-39
GTAAT 6326490 6.0101027 39.409306 110-119
GGTGA 10366945 5.9106107 100.70092 45-49
CCCTG 10189610 5.817709 66.83693 30-34
AATAC 4459245 5.575056 39.480316 110-119
AGACG 7362080 5.523965 102.16413 40-44
GTAGT 7951655 5.396579 32.21064 80-89
ACAGT 5954115 5.3179846 35.416 110-119
GTGAC 8033320 5.125862 84.54369 45-49
GAGAC 6521145 4.892989 141.67178 40-44
CCCCA 6490885 4.8771615 101.50657 60-69
TAGTA 5115570 4.8597403 41.911488 80-89
CTCAG 6652405 4.750517 34.873543 140-149
ACTCC 5803405 4.6380486 115.43274 30-34
ACCAG 5379545 4.5173764 89.20715 45-49
GGCCC 9319745 4.470118 57.586243 60-69
GCACA 5295380 4.4467 34.028187 110-119
TGACC 6147915 4.3902574 145.58417 45-49
GTGTC 8088760 4.3890977 23.503706 120-129
AGTAG 5495875 4.3860784 33.588856 80-89
CGTGT 8000430 4.3411684 22.95976 120-129
CACTC 5321235 4.2527013 95.03995 25-29
CTCCC 6431560 4.109615 66.09132 30-34
CCCAG 6064610 4.0717006 86.70808 60-69
ATGCA 4446475 3.971419 92.7381 25-29
GACCA 4682385 3.9319487 102.90348 45-49
CCCTT 5717965 3.8861125 41.884518 20-24
TGAGG 6725175 3.8342917 68.6787 35-39
TACAC 3791570 3.7900004 27.195745 110-119
AGGAG 5639895 3.7812128 94.76982 35-39
ACGGT 5851315 3.733579 96.97066 40-44
GACGG 6946030 3.7233086 108.28041 40-44
ATGGT 5441285 3.6928568 25.82461 190-199
TGTCC 6038745 3.6671638 20.383059 120-129
AGATA 3278675 3.6626546 19.23328 150-159
CTGAG 5737960 3.6612499 103.704025 35-39
CCACT 4503015 3.5987847 28.41153 240-247
CAGGG 6686025 3.5839372 33.31541 50-59
GAGGA 5320060 3.5667825 124.41678 35-39
TGGTG 7226935 3.5039437 18.919943 25-29
GCCCC 6514635 3.4969976 72.45647 60-69
CTTGG 6427395 3.487613 23.859924 20-24
TGCAC 4873150 3.4799414 111.204956 25-29
AGGCT 5410150 3.4520824 29.31551 30-34
CCGTG 6735260 3.436042 22.981644 120-129
CAGAT 3815695 3.408031 17.68858 150-159
GGATG 5858405 3.340111 67.48299 20-24
CCTGA 4661445 3.3287613 81.50937 30-34
GGGTT 6846130 3.3193123 39.49172 50-59
TCTTG 5125325 3.3105044 17.967289 170-179
CTCTC 4853305 3.2984622 13.56214 100-109
TCAGG 5118745 3.266144 20.806162 140-149
CTTCA 3786115 3.2183661 16.5981 200-209
GCACT 4481990 3.200612 86.159134 25-29
CCAGG 5329680 3.197303 37.86379 50-59
TCGCA 4461280 3.185823 23.658577 100-109
TCTCA 3693565 3.1396945 21.642843 130-139
TGTCT 4828810 3.118982 19.640661 180-189
CCTGG 6091160 3.1074495 27.83247 50-59
ACAGA 2941465 3.0893857 14.410133 140-149
CGCAC 4599340 3.0879374 24.126162 100-109
CGGAT 4687820 2.9911816 76.71664 20-24
GCCCT 5195325 2.966246 22.321869 20-24
GGTTG 6117375 2.965979 70.385925 15-19
TTCCC 4265145 2.8987296 47.05035 50-59
TGGCC 5664930 2.8900054 42.158752 60-69
GACTC 4022320 2.8723593 19.727045 190-199
GATGC 4501165 2.8720813 92.03598 25-29
GATGG 5030470 2.8680723 22.907269 15-19
GGTTC 5255185 2.851552 44.67541 50-59
TCTCT 3914445 2.8296535 14.883607 180-189
AGGGT 4933615 2.8128514 47.141376 50-59
CGGTG 6126405 2.7926693 67.99968 40-44
ACCAC 2963440 2.7850127 19.069721 230-239
TCAAA 2216660 2.7713223 55.88808 70-79
GCCGT 5418735 2.7644074 21.715408 120-129
TTCTT 3571130 2.7457302 20.614286 170-179
GTTGG 5648025 2.7384171 50.71121 15-19
TCCAT 3217870 2.7353325 21.04969 70-79
CGGCC 5620850 2.695982 12.484997 120-129
TCACA 2689110 2.6879969 11.695448 140-149
AGTCA 2952630 2.6371746 29.134544 70-79
AGATG 3280415 2.6179922 16.895144 180-189
CCAGT 3661415 2.6146352 33.95073 60-69
TGGAG 4555940 2.597524 20.073982 25-29
GTTCC 4276410 2.5969462 50.67246 50-59
GGCTG 5693090 2.5951462 15.107549 30-34
AGGGA 3802580 2.5494027 9.1093025 150-159
TCCTC 3711065 2.522159 16.720243 130-139
TGTTC 3819775 2.467235 12.677113 140-149
ACTCT 2897765 2.463229 13.978622 200-209
GCGGA 4570270 2.4498205 83.41054 20-24
ACAGC 2864690 2.4055722 10.944544 120-129
GAGAT 3012320 2.4040344 16.426405 180-189
AATAA 1524265 2.3835053 10.469886 110-119
GGCGG 6191305 2.3709178 39.92518 20-24
TGTAG 3491830 2.3698132 8.188651 210-219
GTTCT 3643030 2.3530736 17.319593 160-169
CAGCT 3270345 2.33537 12.210625 150-159
CTGGA 3643100 2.3245716 14.401777 180-189
TTGGG 4754475 2.3051836 76.454094 15-19
CACGG 3836830 2.3017344 9.660408 120-129
TACCA 2282650 2.2817051 16.263021 230-239
CATTT 2507865 2.2674356 17.558857 150-159
TCATT 2488455 2.2498863 16.32549 150-159
CAGAG 2991585 2.244666 9.320126 140-149
CTGGC 4397870 2.2436056 32.55633 60-69
TTCAT 2477260 2.2397645 15.745561 140-149
TCAGC 3094010 2.2094483 14.351154 140-149
GTGAA 2731695 2.1800768 17.704844 190-199
TTGTC 3369205 2.176207 13.051131 170-179
ATGTA 2277975 2.1640534 9.416156 70-79
GATAC 2421285 2.162598 14.359611 150-159
CTACT 2525130 2.1464727 7.7271266 230-239
TTCAC 2512925 2.1360977 18.198915 200-209
CCAGA 2542825 2.1352916 31.781763 60-69
ATTTG 2633000 2.1271164 15.421356 150-159
CTCGC 3712510 2.1196399 16.20156 100-109
ACTAC 2109250 2.1083767 12.584802 230-239
GAACT 2359105 2.107061 10.919992 160-169
TCTCG 3421370 2.077704 15.940643 100-109
ATCTC 2441215 2.0751412 8.160654 100-109
TCTGC 3390920 2.0592124 12.024427 210-219
CACTA 2060000 2.0591471 15.246174 240-247
GCTGT 3794030 2.0587046 9.930196 140-149
GAATC 2303025 2.0569727 14.448236 190-199
CTCTG 3380430 2.0528421 14.383212 180-189
AGCTC 2872160 2.0510242 12.342191 140-149
GTCCT 3372205 2.0478473 14.128008 130-139
TTGGA 3003590 2.0384574 15.309917 170-179
TACAG 2277990 2.0346124 13.501143 160-169
ACACA 1724985 2.0276084 13.552578 110-119
TGGGG 4964835 2.0222197 50.09685 15-19
TGAAT 2127450 2.0210564 13.98326 190-199
GTCTC 3325230 2.0193205 11.678647 180-189
GATAT 2118785 2.0128245 8.322128 180-189
GTTCA 2647990 2.0112574 14.694702 140-149
CAGGC 3342635 2.0052643 15.109156 140-149
ACACG 2381930 2.000183 13.613763 120-129
TAGTC 2626795 1.9951589 24.016651 70-79
TTTGC 3078030 1.9881334 11.795544 150-159
ACGGC 3313795 1.9879631 14.302385 120-129
AATCG 2222825 1.9853411 13.417881 190-199
ATATA 1482505 1.9713931 10.297289 110-119
TTGCA 2586205 1.9643291 13.911705 150-159
TGGAA 2457930 1.9615939 8.733548 170-179
AATAT 1460605 1.9422711 12.882676 110-119
GACGT 3040340 1.9399655 10.076296 60-69
AACCA 1647995 1.9371116 14.635282 70-79
CCTTG 3171150 1.9257518 27.169947 20-24
ATATG 2007380 1.9069909 8.548589 180-189
GTCAA 2134550 1.9064974 37.351112 70-79
CAGCC 2820390 1.8935734 9.972561 120-129
CCTTC 2771415 1.8835425 13.614928 200-209
GAGTC 2925535 1.8667111 12.153202 210-219
CATAG 2080545 1.8582622 8.498929 210-219
TCGGC 3626040 1.8498508 7.479181 130-139
TACTA 1739730 1.8496569 10.388195 230-239
GGGGC 4697010 1.7986879 45.48645 15-19
AGAAC 1705965 1.7917547 11.472699 160-169
CTGTT 2772200 1.7905945 12.357355 140-149
GTTGT 3077000 1.7758648 7.107592 210-219
AGTAT 1848100 1.7556765 11.971371 220-229
GGGCG 4579980 1.7538722 55.605038 20-24
CTCCA 2191320 1.7512908 7.983886 150-159
CGTAG 2743590 1.7506167 9.678656 210-219
GTCCC 3061525 1.7479631 23.393375 50-59
CACCA 1859775 1.7477988 13.230851 230-239
GTCAC 2421470 1.7291842 8.625035 130-139
GGTCA 2701880 1.7240026 7.612724 130-139
ATTGT 2132175 1.7225161 8.541612 50-59
GCAGA 2287675 1.7165036 13.9842005 150-159
TTGGT 2954850 1.705367 28.63579 25-29
TGACT 2234690 1.6973389 11.934888 190-199
TCAGA 1892050 1.6899058 10.5306225 130-139
TGCAG 2647810 1.6895019 12.987832 150-159
CAAAA 1135770 1.669771 22.381077 70-79
ATAGT 1751570 1.663974 7.5190043 220-229
AACTG 1857410 1.6589667 10.730134 160-169
AGTCT 2182500 1.6576985 12.893365 210-219
CAGAC 1969550 1.6538943 19.228735 60-69
GAGAA 1760615 1.6522758 10.636865 160-169
TCCCA 2066705 1.6516992 11.61261 240-247
TGGTT 2839615 1.6388601 17.543291 10-14
TTCAG 2154235 1.6362301 9.386218 150-159
CTGTG 3002485 1.6291989 5.5957274 210-219
AATAG 1430680 1.598233 9.561127 240-247
GCTCC 2776540 1.5852522 6.355071 150-159
TGCTC 2605285 1.5821178 5.509292 150-159
TGTGC 2913520 1.5809251 8.211346 220-229
ACTGA 1769115 1.580105 8.754204 180-189
GCTCA 2205945 1.5752766 11.136602 140-149
AAAAA 856445 1.57483 8.085597 8
ATAGA 1408765 1.5737514 10.436216 240-247
CTGGT 2893020 1.5698014 6.933612 160-169
ACCAT 1569005 1.5683556 12.983523 45-49
GTCTG 2877240 1.5612389 8.838844 210-219
GGCCG 3631500 1.5563623 12.792647 120-129
GCTGA 2439115 1.5563388 24.7951 30-34
TTGTA 1924165 1.5544716 9.203809 210-219
TCTGG 2857065 1.5502917 11.587263 180-189
GAACC 1835350 1.5412022 11.947879 70-79
TAGTT 1906480 1.5401843 9.939823 220-229
GGACG 2872695 1.5398624 7.1704288 170-179
CATGT 2026615 1.5392975 9.388897 70-79
GTGCT 2813325 1.5265576 7.929404 220-229
CAAAG 1421985 1.4934939 26.707464 70-79
AGATC 1660470 1.4830674 7.223374 130-139
ACCGT 2074390 1.4813325 22.675812 45-49
CCCAC 1969155 1.4795961 10.629394 240-247
CCTCG 2577580 1.4716569 9.270895 130-139
GTGGA 2573860 1.4674606 25.392433 25-29
CCACC 1948580 1.4641364 9.411649 230-239
GCTCT 2375535 1.4425969 9.531948 130-139
CGACT 2007810 1.4337873 8.70507 190-199
TCGAC 1972175 1.4083402 9.33152 190-199
CCTCA 1757380 1.4044883 7.4843464 130-139
TGATA 1477760 1.4038574 7.719104 180-189
CCATG 1956325 1.3970217 9.48842 70-79
GTCCA 1951765 1.3937653 19.579483 70-79
TGGAC 2180405 1.3912623 10.292347 170-179
CTCTT 1923525 1.3904676 8.260906 200-209
CACTG 1925075 1.3747059 7.456745 230-239
GTAGG 2387420 1.3611637 5.3082952 160-169
TATAC 1275215 1.3557911 6.5268416 110-119
AATTG 1422495 1.351356 8.711864 170-179
GGGAG 2806960 1.3444304 6.4983735 150-159
CTGCT 2193530 1.3320704 5.4250717 90-99
CTCGA 1857020 1.3261076 8.369693 190-199
ACTTC 1559325 1.3254955 6.8993964 220-229
GAGGG 2763190 1.3234662 8.083645 200-209
GGAGG 2757405 1.3206954 18.982292 30-34
GCGTA 2068270 1.3197118 10.240302 210-219
TATGG 1943595 1.3190666 6.2490816 180-189
CCATC 1650415 1.3190024 11.373891 240-247
TGGTC 2419820 1.3130351 14.522834 50-59
GTGGT 2702265 1.3101798 12.8612995 50-59
CTGAT 1724795 1.3100528 7.3404574 180-189
CTTGA 1705190 1.295162 8.44459 200-209
TTGGC 2373875 1.2881047 9.311554 60-69
TCCAC 1601640 1.2800218 9.947767 240-247
ACGGA 1700685 1.2760693 9.05028 200-209
AGCCG 2125855 1.2753116 8.8659115 120-129
ACGTG 1990955 1.270379 9.268598 170-179
GTGTT 2185555 1.2613748 7.2902274 160-169
GAATT 1327155 1.260784 9.683843 170-179
ATCGG 1975480 1.2605047 7.9272027 190-199
TGCCC 2186655 1.2484602 8.142002 60-69
GATCT 1635235 1.2420282 5.712949 130-139
AAGTA 1102770 1.2319201 15.261587 70-79
ACTAT 1155330 1.228331 6.551601 230-239
ACTCG 1709875 1.2210305 8.515993 190-199
GGCTC 2381770 1.2150773 7.921433 130-139
ATAAA 773190 1.2090434 5.2808294 8
CCATA 1204295 1.2037965 12.065691 70-79
GGAGT 2109565 1.2027475 6.752901 210-219
ATAAC 955985 1.1951956 7.07452 80-89
ACTGG 1869415 1.1928273 7.048358 160-169
GGAAT 1493235 1.1917021 8.114708 170-179
TTGAG 1749160 1.1871089 8.148004 200-209
ATACC 1183410 1.1829201 8.749255 70-79
ATGGG 2063140 1.1762786 36.036446 15-19
GCATA 1307765 1.168045 8.08299 210-219
AGCTT 1525465 1.1586534 6.174385 150-159
AGAGC 1542360 1.1572739 6.942545 140-149
AAAGT 1034715 1.1558949 19.482958 70-79
ACTGT 1517735 1.1527822 5.7326756 230-239
TATCA 1082385 1.1507767 7.4648185 70-79
GAAGA 1223825 1.1485171 19.799786 35-39
AGTTC 1501025 1.1400902 5.523761 160-169
TCTGT 1744960 1.127089 6.750978 210-219
AAAAG 857325 1.1262159 15.2921 70-79
TTCCA 1324770 1.1261134 7.508831 230-239
AGAGA 1199205 1.1254121 14.606351 35-39
CAATA 896760 1.1211511 6.6589413 100-109
ATATC 1044270 1.1102535 7.940861 70-79
TCTAC 1303480 1.1080159 7.3101482 180-189
CTGAA 1239410 1.106993 15.925962 35-39
GTCTA 1455720 1.1056793 7.487487 180-189
AGGTT 1627670 1.1046568 22.791096 10-14
ATCCA 1095370 1.0949166 16.366293 240-247
AACTT 1022710 1.0873312 6.4799027 200-209
TCACG 1515880 1.0824977 9.042897 200-209
TACTG 1398725 1.0623891 6.386712 180-189
CTCGG 2075785 1.058977 7.867387 130-139
CGGCT 2065080 1.0535158 7.4390054 130-139
GAGCT 1650945 1.053427 6.2171283 140-149
GTCCG 2059550 1.0506945 5.0912166 130-139
GCTTC 1724485 1.0472323 5.4668136 150-159
CTGCC 1828770 1.0441275 5.956909 200-209
ACGTC 1444475 1.031507 13.443821 70-79
CATGG 1612475 1.0288802 5.4797373 190-199
GCGGT 2241870 1.0219373 5.739685 130-139
CGTGG 2233290 1.0180262 8.775004 50-59
TAGAT 1069235 1.0157626 8.01133 240-247
TATGT 1253550 1.012703 7.4455376 220-229
CTTCT 1398400 1.0108681 5.7082376 210-219
TCCCC 1570655 1.0036114 5.3849554 80-89
CTGCG 1961545 1.0006965 6.6742244 210-219
AAGAG 1064910 0.9993809 14.841055 35-39
CGAAC 1165550 0.97874975 12.570212 70-79
AAAAT 620205 0.9698195 5.130699 8
CTACC 1207365 0.9649194 8.211835 230-239
ACAAT 770905 0.9638041 7.692345 100-109
TATAA 724780 0.96379185 6.592645 240-247
CCATT 1132730 0.9628708 6.419966 50-59
TAGGC 1508925 0.9628075 5.948214 160-169
TGCGT 1760650 0.9553584 8.114083 210-219
GAGGC 1780350 0.95432824 16.175257 30-34
GGTGG 2342145 0.9539756 25.069248 25-29
AGCTG 1484505 0.9472258 5.0761766 30-34
GGGGT 2322445 0.94595164 7.1049366 10-14
TGAAG 1184790 0.94554234 10.575835 35-39
GAGTT 1381110 0.937323 5.2759066 160-169
CCCAA 994275 0.9344102 10.097175 240-247
ACCCA 987460 0.9280055 12.957456 240-247
CAGCG 1521770 0.91291785 5.8515844 160-169
AGTTT 1120480 0.90519994 5.030408 220-229
TCGAA 1010815 0.90282077 16.528837 70-79
ATCAA 721795 0.9024056 14.242421 70-79
GGGCC 2101990 0.90085584 18.925625 15-19
CGGAG 1675760 0.8982644 6.298459 210-219
CGTCC 1569995 0.89638114 10.930205 70-79
TGCAT 1178800 0.8953471 6.1968203 210-219
TGTAA 938825 0.89187443 6.750801 230-239
GACCG 1484505 0.89056236 20.615372 45-49
TTCTG 1370195 0.8850242 5.625606 210-219
ACTAA 692890 0.8662679 12.364352 240-247
TGGGC 1892040 0.86247027 20.594343 15-19
CATCA 854450 0.8540963 5.585775 230-239
CCAAT 853060 0.8527069 10.945347 240-247
CGGTC 1665480 0.8496568 5.4756103 130-139
GGTCC 1663590 0.8486926 14.533428 50-59
TGAAC 929930 0.8305775 6.181568 190-199
CCAGC 1229580 0.82552415 5.482355 80-89
AGCGT 1253155 0.7996071 6.0438137 160-169
GTATG 1175170 0.79755694 5.227475 220-229
CCCCC 1318085 0.7918427 5.028299 80-89
CTTCC 1137415 0.7730238 5.2461267 230-239
TAACC 772120 0.77180034 5.6356215 80-89
CATAC 763245 0.762929 10.466145 70-79
AACCG 895670 0.7521228 5.370382 190-199
TGAGA 938470 0.74896234 5.7229095 240-247
AAAGA 567240 0.74514884 5.736944 8
AAAGG 788870 0.740327 8.637855 10-14
CCTTT 1018430 0.7361973 5.6192727 50-59
AATCC 730065 0.7297628 9.766016 240-247
AGCCA 867050 0.72808963 5.587037 80-89
TCCAG 1015730 0.72533804 5.0065374 240-247
ATGAT 758295 0.7203727 7.071481 240-247
GTCGA 1123525 0.71689343 11.374863 70-79
AAAGC 680225 0.71443224 10.388522 70-79
GTAGC 1114140 0.7109051 5.413493 80-89
CATTG 915905 0.6956675 5.873921 50-59
AAGGT 871455 0.69547975 9.661393 10-14
CTAAT 653565 0.6948613 9.45042 240-247
TGTTA 857990 0.6931428 5.2080145 230-239
CGATG 1084250 0.691833 15.864391 15-19
GGAAG 1028180 0.68933326 6.912383 30-34
AGGAT 845180 0.6745106 6.87957 10-14
TAGGT 988100 0.67059743 5.357619 10-14
ATCCC 829655 0.66305566 5.03985 240-247
CCGTA 918585 0.6559662 5.496303 80-89
GAGGT 1135125 0.64718026 6.668658 10-14
CCCAT 808810 0.64639646 5.1101885 240-247
AGACC 761925 0.6398128 5.030812 240-247
TATGA 670940 0.6373863 5.875689 240-247
AGAAG 675735 0.6341537 5.4103436 9
CAATC 627680 0.6274202 9.459871 240-247
ATAGG 771230 0.6154935 5.25047 10-14
CATCC 742230 0.5931861 7.971044 240-247
AATGG 709115 0.56592155 6.518314 10-14
TAAAA 355080 0.55524147 5.7987785 8
GGGTC 1216375 0.5544742 5.385737 70-79
AAATG 489080 0.5463582 5.446884 9
AAGCA 520155 0.5463126 7.0674148 70-79
GAAGG 793545 0.5320245 5.4158826 10-14
AAGAA 404245 0.53103215 5.3583503 9
GAAAA 402940 0.52931786 6.020717 8
ACGAT 570280 0.50935197 7.059198 10-14
TTGCC 835475 0.507361 5.744002 60-69
AAGGG 748135 0.5015798 5.9967823 10-14
TGATC 650185 0.49384224 5.552854 240-247
GGTCG 1041275 0.4746563 5.4569945 70-79
ATAAG 424270 0.47395805 5.686853 9
GATCC 659555 0.4709916 5.566651 240-247
TACCG 632095 0.45138228 5.514427 70-79
GAAGC 597665 0.448444 5.560929 30-34
TAAGG 545470 0.4353218 5.8153396 10-14
AAGCT 484460 0.43270087 9.372033 30-34
CGGTT 797155 0.43254974 7.828283 10-14
TAAAG 354790 0.39634097 5.546089 9