FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_12_SL248341.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_12_SL248341.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9542239
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10754020 8.824811 62.47213 110-119
ATACA 9804440 7.6098685 38.285854 110-119
CACAG 14402725 7.570928 42.51106 110-119
TCCCT 17079675 7.304929 108.05452 30-34
CAGTA 12297905 6.8346434 39.71336 110-119
AGTAA 9390185 6.484726 48.192245 110-119
GGAGA 15018185 6.2495413 92.81298 35-39
GTAAT 10479905 6.1577544 40.266705 110-119
CCCTG 17081890 6.148301 70.21467 30-34
GGTGA 16630220 5.8881145 100.834236 45-49
AATAC 7135495 5.5383253 40.285263 110-119
AGACG 11347035 5.3070107 101.15026 40-44
ACAGT 9469195 5.2625685 36.163094 110-119
GTAGT 12448380 5.23731 26.692581 80-89
GTGAC 12802545 5.094616 83.74746 45-49
GAGAC 10460745 4.8924932 141.13997 40-44
ACTCC 9719240 4.885636 123.397484 30-34
CCCCA 10185790 4.842875 101.62671 60-69
ACCAG 9059500 4.7622113 94.59455 45-49
CTCAG 10519110 4.70469 35.538963 140-149
GCACA 8564770 4.502152 34.747578 110-119
CACTC 8905695 4.476686 99.69498 25-29
TAGTA 7566990 4.4461913 34.005997 80-89
GGCCC 14603210 4.4233418 59.20093 60-69
GTGTC 12946845 4.3835564 24.034649 120-129
TGACC 9788345 4.377854 143.28766 45-49
CGTGT 12872850 4.358504 23.432255 120-129
CTCCC 10563725 4.2733965 70.25655 30-34
AGTAG 8537820 4.221773 27.883669 70-79
ATGCA 7520310 4.179463 97.02358 25-29
GACCA 7890040 4.147474 106.83146 45-49
CCCAG 9588220 4.0561094 86.52372 60-69
TGAGG 10915825 3.8648694 68.586365 35-39
AGGAG 9115540 3.7932649 93.30441 35-39
TACAC 6043505 3.7749438 28.012106 110-119
CAGGG 11269810 3.77411 36.070133 50-59
ACGGT 9384020 3.7342558 95.26475 40-44
GACGG 11085765 3.7124758 106.80295 40-44
CCACT 7384905 3.71222 29.502745 240-247
CTGAG 9270835 3.6892154 105.07585 35-39
ATGGT 8742875 3.6783216 26.3188 190-199
TGCAC 8192865 3.6642723 116.83574 25-29
TGTCC 9501455 3.615677 20.906025 120-129
AGATA 5205455 3.594812 19.599352 150-159
GAGGA 8574695 3.568202 122.8272 35-39
GGGTT 11774925 3.5471857 42.846474 50-59
CCCTT 8219010 3.515247 33.961487 20-24
CCTGA 7803425 3.4900951 84.98576 30-34
GCCCC 10213335 3.477015 72.78845 60-69
CCAGG 9065220 3.4120328 41.070827 50-59
GCACT 7601740 3.3998911 90.08391 25-29
GGATG 9548610 3.3807917 69.898125 20-24
TGGTG 11153270 3.3599129 18.229565 190-199
TCTTG 8294850 3.3372467 18.505243 170-179
CTTCA 6262875 3.3284564 17.273434 200-209
CCGTG 10313805 3.3029444 23.679852 120-129
CTCTC 7712130 3.2984564 13.635911 130-139
AGGCT 8221650 3.271705 23.529356 30-34
CTTGG 9653815 3.2685986 18.89816 20-24
TCAGG 8181215 3.2556143 21.13759 140-149
CAGAT 5849135 3.2506962 18.016905 150-159
CCTGG 10142625 3.248125 29.735735 50-59
TCGCA 7188105 3.2148921 24.411737 100-109
TGTCT 7841945 3.1550305 20.316916 180-189
TTCCC 7343490 3.14079 51.05292 50-59
CGGAT 7888010 3.138937 79.800285 20-24
CGCAC 7381370 3.122545 24.994862 100-109
TCTCA 5865560 3.1173 21.975155 130-139
GGTTG 10270730 3.0940485 74.30324 15-19
GGTTC 8969070 3.0367572 48.587856 50-59
ACAGA 4638695 3.0299327 14.94386 140-149
GATGC 7605520 3.0265234 97.43578 25-29
AGGGT 8433545 2.9859905 50.566463 50-59
TGGCC 9312165 2.9821744 43.73459 60-69
TCAAA 3742350 2.904683 59.10195 70-79
AGTCA 5194615 2.8869421 32.65599 70-79
GTTGG 9554520 2.878291 53.0419 15-19
ACCAC 4844820 2.862325 20.346508 230-239
GACTC 6384220 2.855353 20.41294 190-199
CCAGT 6383100 2.8548522 37.62418 60-69
TCTCT 6268795 2.8346584 15.385303 180-189
CGGTG 9818995 2.7977736 66.87963 40-44
GCCCT 7768570 2.7961495 18.378355 20-24
GCCGT 8697055 2.7851884 22.365005 120-129
TTCTT 5794405 2.7701657 21.222803 170-179
GTTCC 7263410 2.764013 55.23951 50-59
GATGG 7625670 2.6999533 18.643576 15-19
CGGCC 8898955 2.6955113 12.78024 120-129
TCACA 4273000 2.6690364 12.27073 140-149
AGATG 5278390 2.610053 17.34809 180-189
GCGGA 7721040 2.5856743 87.423836 20-24
AGGGA 6129460 2.5506623 9.387053 150-159
TCCAT 4741265 2.5197842 16.548515 70-79
TCCTC 5836275 2.4961584 17.134895 130-139
GGCTG 8696855 2.4780366 12.086271 30-34
TGTTC 6150685 2.4745903 13.14059 140-149
GGCGG 10319685 2.4745421 42.07657 20-24
ACAGC 4649300 2.4439483 11.292654 120-129
TGGAG 6884425 2.4375074 15.871113 25-29
ACTCT 4570370 2.4289608 14.294684 200-209
TTGGG 8053150 2.4260044 79.72985 15-19
CTGGC 7550280 2.41794 35.496983 60-69
GAGAT 4820735 2.3837523 16.904682 180-189
CAGCT 5317875 2.3784287 12.660072 150-159
GTTCT 5899800 2.373652 18.037739 160-169
TACCA 3767360 2.3531992 16.96459 230-239
AATAA 2419665 2.3336854 9.913468 110-119
CACGG 6144275 2.3126266 9.766863 120-129
CTGGA 5757010 2.2909317 14.730699 180-189
CATTT 4055155 2.2785408 17.910206 150-159
TCATT 4041415 2.2708206 16.716097 150-159
TCAGC 5075900 2.2702048 14.841681 140-149
TGTAG 5391240 2.2682145 8.439989 210-219
TTCAT 3984345 2.2387538 16.549664 140-149
CTACT 4178560 2.22073 7.9345183 230-239
CAGAG 4731755 2.213043 9.850792 140-149
ACTAC 3506655 2.1903555 12.991913 230-239
TTCAC 4109520 2.1840382 18.995327 200-209
GTGAA 4373955 2.162829 18.067423 190-199
GATAC 3881225 2.157017 14.811759 150-159
CACTA 3445665 2.1522593 16.041475 240-247
TTGTC 5325105 2.1424367 13.522964 170-179
TGGGG 8420725 2.1348045 52.14129 15-19
TAGTC 4513715 2.1343539 26.90282 70-79
ATTTG 4253155 2.1262963 15.7930765 150-159
CTCGC 5901985 2.1243074 16.612467 100-109
GAACT 3820610 2.1233296 11.481443 160-169
TCTCG 5550860 2.1123204 17.015968 100-109
TCTGC 5549295 2.1117249 12.461615 210-219
ATCTC 3960285 2.104726 8.977296 100-109
TTGGA 4952515 2.083633 16.19621 170-179
GTCAA 3736980 2.0768516 41.397198 70-79
AGCTC 4613160 2.0632436 12.823112 140-149
GCTGT 6056160 2.0505013 10.222652 140-149
GATAT 3474075 2.0412877 8.520002 180-189
GTCCT 5362575 2.0406704 14.478835 130-139
GTCTC 5358295 2.039042 11.989218 180-189
ATATA 2479185 2.0344336 10.492577 110-119
GTTCA 4301310 2.0339158 15.289536 140-149
ACACG 3859830 2.028956 13.708166 120-129
CTCTG 5327710 2.027403 14.721683 180-189
TACAG 3647310 2.027017 13.847807 160-169
GAATC 3644195 2.025286 14.659572 190-199
ATGTA 3444895 2.024142 7.3428087 160-169
ACACA 2745565 2.0156066 13.830152 110-119
CAGGC 5330320 2.0062642 15.438961 140-149
AACCA 2715815 1.9937665 15.400265 70-79
AATAT 2418920 1.9849801 13.212393 110-119
TGAAT 3361435 1.9751029 14.025418 190-199
TTGCA 4162805 1.9684224 14.443708 150-159
ACGGC 5229320 1.9682492 14.574864 120-129
AATCG 3515920 1.9539962 13.460309 190-199
AAAAA 1721185 1.9510442 10.567639 120-129
TGGAA 3879085 1.9181262 9.316156 170-179
CCTTC 4480525 1.9163079 14.220432 200-209
TTTGC 4752230 1.9119532 12.31144 150-159
ATATG 3247385 1.9080896 8.815871 180-189
GGGGC 7950225 1.9063727 47.53979 15-19
TACTA 2885685 1.9056836 10.384547 230-239
CAGCC 4499250 1.90332 10.292653 120-129
GAGTC 4730205 1.8823271 12.559686 210-219
TCGGC 5812500 1.8614243 7.872789 130-139
GGGCG 7738520 1.8556082 59.183056 20-24
CATAG 3335915 1.853957 8.610528 210-219
AGAAC 2740290 1.7899203 12.069105 160-169
GACGT 4491990 1.7875323 10.060696 170-179
CCAGA 3392695 1.7834021 25.558405 60-69
GTCAC 3979390 1.7797886 8.937384 130-139
CTGTT 4417230 1.7771735 12.905618 140-149
CTCCA 3534810 1.7768672 7.9914856 150-159
AGTAT 3016005 1.7721361 12.246484 220-229
GTTGT 4935050 1.7665848 7.376026 210-219
CCTTG 4609735 1.7541854 21.703588 20-24
CGTAG 4357930 1.7341849 9.985131 210-219
TTGTA 3431565 1.7155555 9.829277 210-219
GCAGA 3667890 1.7154729 14.567111 150-159
ATTGT 3414045 1.7067968 7.563091 170-179
GGTCA 4288590 1.7065918 7.9204955 130-139
TGCAG 4286265 1.7056665 13.702758 150-159
TTCAG 3594000 1.6994576 9.824491 150-159
CACCA 2845410 1.6810714 14.006614 230-239
AACTG 3024550 1.680914 11.322679 160-169
AGTCT 3552360 1.6797677 13.545133 210-219
TGGTT 4682755 1.6762714 18.051378 10-14
TGACT 3538175 1.6730603 12.215514 190-199
GAGAA 2845985 1.6539904 10.93924 160-169
ATAGT 2813685 1.6532576 7.681848 220-229
TCAGA 2950770 1.6399102 10.156666 130-139
TCCCA 3259070 1.6382589 11.38207 240-247
GTCTG 4824425 1.6334587 9.300412 210-219
CAAAG 2483525 1.6222049 29.756855 70-79
CAAAA 1771260 1.6158056 21.094393 70-79
ACTGA 2879905 1.6005265 9.250121 180-189
GCTCC 4430945 1.5948344 6.3738227 150-159
TGCTC 4172515 1.587806 5.2949414 150-159
CTGGT 4677795 1.583813 7.3348536 160-169
AATAG 2287040 1.5793968 10.001864 240-247
CTGTG 4660945 1.5781077 5.424431 210-219
GAACC 2996825 1.5753093 12.690886 70-79
TCTGG 4639530 1.5708568 12.0439415 180-189
GCTCA 3511855 1.5706831 11.477178 140-149
TAGTT 3131060 1.5653229 10.372475 220-229
ATAGA 2265305 1.564387 10.80906 240-247
TGTGC 4617450 1.5633811 8.389004 220-229
TTGGT 4364425 1.5623199 23.271042 25-29
GTGCT 4610850 1.5611465 8.504046 220-229
GTCCC 4320515 1.5550871 19.264133 50-59
GGCCG 5737055 1.5461594 13.084156 120-129
GGACG 4602455 1.5413013 7.483937 170-179
ACCAT 2408550 1.5044482 11.325283 45-49
CCCAC 3107820 1.4776256 10.765986 240-247
CCACC 3106835 1.4771572 10.120134 230-239
CCTCG 4076555 1.4672787 9.675682 130-139
GCTCT 3848720 1.4645892 10.006923 130-139
CGACT 3234655 1.446705 9.076444 190-199
CAGAC 2701715 1.420182 14.735512 60-69
TGATA 2415985 1.419578 8.056928 180-189
TATAC 2147700 1.4183241 6.7082 110-119
TCGAC 3169590 1.4176044 9.833921 190-199
CATGT 2988880 1.4133207 7.2243733 70-79
GCTGA 3526030 1.4031405 19.81101 30-34
TGGAC 3525415 1.4028957 10.69779 170-179
CACTG 3131195 1.4004321 7.617029 230-239
AAATA 1451685 1.4001013 5.595619 9
CTCTT 3085870 1.3953857 8.627751 200-209
AATTG 2368840 1.3918766 9.386838 170-179
CTGCT 3630885 1.3816944 5.853816 90-99
AGATC 2479630 1.3780711 7.0755906 130-139
GGGAG 4568745 1.3613131 6.7138357 150-159
GAGGG 4549015 1.3554344 8.442648 200-209
CCTCA 2684110 1.34924 7.4291706 130-139
CTTGA 2832295 1.339278 8.845149 200-209
GCGTA 3365445 1.3392376 10.516081 210-219
CTCGA 2968575 1.3277003 8.805032 190-199
CTGAT 2807180 1.3274022 7.73041 180-189
GTAGG 3704270 1.3115381 5.183802 160-169
CCATC 2604590 1.3092668 11.245468 240-247
ACTTC 2457360 1.3059841 6.8994904 220-229
ACGGA 2785805 1.3029217 9.462501 200-209
TATGG 3082730 1.296973 6.2875643 180-189
GAATT 2206315 1.2963806 10.382487 170-179
ATCGG 3229480 1.2851319 7.948217 190-199
TCCAC 2555065 1.284372 10.110752 240-247
GTGGA 3622040 1.2824236 20.067104 25-29
CCATG 2865740 1.2817069 7.3305426 70-79
ACGTG 3218720 1.2808502 9.58832 170-179
GGAAT 2578825 1.2751747 8.679303 170-179
AGCCG 3387420 1.2749815 9.111043 120-129
AAAGT 1836775 1.2684503 21.755827 70-79
ACTAT 1915335 1.2648721 6.6202903 230-239
ACCGT 2804180 1.2541742 17.19952 45-49
GTGTT 3492430 1.2501745 7.6335807 160-169
AAGTA 1810035 1.2499841 16.021507 70-79
ACTCG 2775720 1.2414453 9.017932 190-199
GATCT 2601600 1.2301917 5.593436 130-139
GGCTC 3836300 1.2285559 8.323167 130-139
GGAGT 3454235 1.2230104 7.12272 210-219
ATAAC 1572090 1.2202021 7.7259746 80-89
ACTGG 3048245 1.2130117 7.5094166 160-169
GGAGG 4065350 1.2113204 15.375147 30-34
GTCCA 2704525 1.2096032 15.447142 70-79
AGAGC 2584970 1.2089912 7.457653 140-149
TTGAG 2853920 1.2007076 8.533166 200-209
AGCTT 2529010 1.1958668 6.589175 150-159
ATAAA 1235110 1.1912221 5.178584 7
TTGGC 3473180 1.1759529 7.79956 60-69
GTGGT 3867120 1.1649665 9.911402 50-59
GCATA 2093210 1.1633155 8.237278 210-219
TGGTC 3433085 1.1623776 11.342029 50-59
TATCA 1746015 1.1530546 6.8503447 70-79
TTCCA 2158430 1.1471153 7.804684 230-239
ATATC 1728285 1.1413457 7.3700695 70-79
GAAGA 1955930 1.1367204 18.111895 35-39
ACTGT 2401275 1.135466 5.8700256 230-239
TGCCC 3149080 1.1334513 6.28273 60-69
ATACC 1809685 1.1303803 6.981969 70-79
TCTAC 2115600 1.124353 7.798977 180-189
AGGTT 2658120 1.1183301 22.910635 10-14
GTCTA 2364090 1.1178827 7.7751837 180-189
TCACG 2496885 1.116736 9.705552 200-209
AGTTC 2352840 1.1125631 5.4318104 160-169
CAATA 1421640 1.1034279 6.5517664 100-109
TCTGT 2731395 1.0989155 6.6391773 210-219
CTGAA 1973100 1.0965637 14.239721 35-39
GCTTC 2878215 1.095274 5.845156 150-159
CCATA 1751810 1.0942298 9.7921915 70-79
AAAAG 1332705 1.0816929 13.964524 70-79
GAGCT 2715915 1.0807651 6.6479654 140-149
TACTG 2285480 1.0807114 6.8585277 180-189
ATCCA 1725920 1.0780584 16.29193 240-247
TGTAA 1833090 1.077082 7.258192 230-239
CGGCT 3354910 1.0743933 7.8127956 130-139
CTCGG 3344485 1.0710547 8.38623 130-139
GTCCG 3268865 1.0468377 5.1990957 130-139
TATGT 2077045 1.0383852 7.7554154 220-229
AACTT 1564750 1.0333486 6.1368375 200-209
GCGGT 3617855 1.0308529 5.9218354 130-139
CTGCG 3214230 1.0293411 7.0183487 210-219
AGAGA 1770160 1.0287572 12.663246 35-39
CTTCT 2270530 1.0267007 5.6699038 210-219
TAGAT 1743395 1.0243794 8.46276 240-247
CTGCC 2836570 1.0209695 5.934325 200-209
GGGGT 4017660 1.0185487 7.7440343 10-14
ATGGG 2858230 1.0119882 29.05291 15-19
CATGG 2543075 1.0119855 5.4338646 190-199
CTACC 2008895 1.009825 8.866343 230-239
ATACG 1797415 0.9989255 5.1921434 120-129
AGAAA 1217445 0.98814183 5.2219152 6
AAGAG 1695650 0.9854545 13.549974 35-39
CGAAC 1872765 0.98443663 13.399124 70-79
TATAA 1197695 0.9828355 6.7164006 240-247
CAGCG 2589215 0.9745474 6.385214 160-169
AAAAT 1004675 0.96897525 5.428607 8
TGCGT 2849165 0.9646732 8.36349 210-219
AGCTG 2384440 0.9488587 5.1186595 90-99
TCCCC 2336160 0.9450585 5.0723743 80-89
ACAAT 1216620 0.94429845 7.68175 100-109
ACCCA 1590710 0.9397932 13.346406 240-247
TAGGC 2342830 0.93230045 5.848875 160-169
CGGAG 2752665 0.92183113 6.6518826 210-219
TCGAA 1654320 0.91939956 17.043242 70-79
CCATT 1725645 0.91710824 5.773485 50-59
GAGTT 2161705 0.9094773 5.115116 160-169
AGTTT 1813900 0.90683 5.0719147 220-229
CCCAA 1531245 0.90466124 10.318009 240-247
TTCTG 2245240 0.90332186 5.580399 210-219
TGAAC 1616890 0.8985975 6.628401 190-199
CGTGG 3143345 0.8956485 6.910724 50-59
ACGTC 1991945 0.8909008 10.6505995 70-79
ACTAA 1144790 0.88854647 12.825381 240-247
TGCAT 1869330 0.8839307 6.3281927 210-219
TGAAG 1778465 0.87941366 9.245478 35-39
CCAGC 2036350 0.86143816 5.8296432 80-89
CGGTC 2673600 0.8562071 5.7578473 130-139
GTATG 2023880 0.8514913 5.357806 220-229
CCAAT 1362710 0.85118705 11.247206 240-247
CATCA 1360665 0.84990966 5.8612666 230-239
AGCGT 2133320 0.8489285 6.6027412 160-169
ATCAA 1084275 0.84157693 12.856969 70-79
GAGGC 2481220 0.8309278 13.044243 30-34
GGTGG 3263155 0.8272682 20.120884 25-29
CTTCC 1885415 0.8063867 5.5049334 230-239
TAACC 1275535 0.79673517 6.2598863 80-89
GGGCC 2902415 0.7822125 15.120045 15-19
GACCG 2067065 0.7780168 15.890632 45-49
AACCG 1466110 0.7706746 5.8111787 190-199
TCCAG 1717395 0.76810783 5.0370936 240-247
AAAGG 1313625 0.76343447 8.75414 10-14
AGCCA 1439555 0.75671566 6.05186 80-89
CGTCC 2100605 0.7560729 8.707507 70-79
AAAGA 930650 0.75536406 5.878745 8
TGAGA 1526460 0.75480247 5.762419 240-247
GGTGC 2627625 0.7487018 5.096121 220-229
GTCGA 1849100 0.73582655 12.168315 70-79
ATGAT 1246315 0.7323064 7.3609014 240-247
TGGGC 2557845 0.7288191 16.47332 15-19
AATCC 1160070 0.7246125 10.061241 240-247
GGTCC 2251015 0.72087634 11.516466 50-59
CTAAT 1086005 0.71718913 9.859956 240-247
AAGGT 1435820 0.7099829 9.771591 10-14
GTAGC 1764530 0.70217305 5.532837 80-89
AGACC 1318025 0.69283223 5.034472 240-247
GGAAG 1664150 0.6925054 5.9755335 30-34
TGTTA 1355825 0.6778228 5.04425 230-239
AAAGC 1031270 0.6736116 9.538327 70-79
CATAC 1076920 0.67267454 8.377327 70-79
CATTG 1387760 0.65621567 5.2194138 50-59
TATGA 1104535 0.64899975 6.039682 240-247
TAGGT 1538030 0.64708334 5.2961946 10-14
ATCCC 1265600 0.6361878 5.0765476 240-247
CAATC 1017450 0.6355279 9.7558565 240-247
AGGAT 1279390 0.63263154 5.4447837 10-14
GAGGT 1776620 0.62903214 6.767189 10-14
CCCAT 1245840 0.6262548 5.07299 240-247
AGAAG 1056185 0.61381906 5.1987658 9
ATAGG 1233200 0.6097916 5.1707883 10-14
CGATG 1495390 0.5950721 13.04638 15-19
CCGTA 1328825 0.59431916 5.0457687 80-89
GGGTC 2079670 0.59257036 5.8411117 70-79
AATGG 1170530 0.5788026 6.579079 10-14
GAAAA 705540 0.57265306 6.1951513 8
AAATG 810555 0.5597576 5.060303 9
CATCC 1101345 0.5536205 7.6202826 240-247
TAAAA 561595 0.5416395 6.2243876 8
GAAGG 1295225 0.5389841 5.430476 10-14
AAGAA 655235 0.531823 5.3301663 9
AAGGG 1242385 0.5169957 6.2119875 10-14
AAGCA 780135 0.5095737 6.306502 70-79
GGTCG 1744875 0.49717563 5.9017925 70-79
TGATC 1023460 0.48395294 5.612287 240-247
GATCC 1046810 0.46818754 5.716819 240-247
ATAAG 670370 0.46294788 5.4261184 9
ACGAT 809760 0.45002958 5.831774 10-14
CGGTT 1313390 0.44468898 8.02192 10-14
TAAGG 896880 0.4434884 5.680938 10-14
TAAAG 585750 0.40451053 5.591272 9
AAGCT 705750 0.39222533 7.5229063 30-34