FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_2_SL248348.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_2_SL248348.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12890719
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14883035 9.048583 63.34363 110-119
ATACA 13815015 7.918587 37.980316 110-119
CACAG 19945485 7.7025795 43.45683 110-119
TCCCT 23231710 7.183392 106.507904 30-34
CAGTA 16768095 6.8685975 40.337307 110-119
AGTAA 12988915 6.665573 49.28249 110-119
GGAGA 20764990 6.427762 96.56623 35-39
GTAAT 14048935 6.104084 40.801056 110-119
GGTGA 22912190 6.0049205 103.97692 45-49
CCCTG 22738475 5.9345155 69.59172 30-34
AATAC 9809070 5.6224313 40.1645 110-119
AGACG 15943150 5.5123143 104.043495 40-44
GTAGT 17689660 5.492685 31.078718 80-89
ACAGT 13284955 5.4418235 36.762985 110-119
GTGAC 17468655 5.1136603 86.47486 45-49
GAGAC 14155950 4.8943934 144.10938 40-44
TAGTA 11233840 4.880961 40.238327 80-89
CCCCA 14145025 4.8702006 102.683495 60-69
CTCAG 14543520 4.7552533 35.28457 140-149
ACTCC 12916125 4.7170243 120.71269 30-34
ACCAG 11856530 4.578774 93.7 45-49
GGCCC 20574120 4.532321 59.169445 60-69
GCACA 11597910 4.4789 35.129177 110-119
CGTGT 17931340 4.444242 23.646929 120-129
AGTAG 12095135 4.4357123 32.493195 80-89
TGACC 13479625 4.4073944 147.68655 45-49
GTGTC 17665945 4.378464 23.989557 120-129
CACTC 11954170 4.3657146 98.528725 25-29
CTCCC 14402515 4.1984987 68.966 30-34
ATGCA 10039550 4.1124306 97.051834 25-29
CCCAG 13262245 4.0881653 88.03944 60-69
GACCA 10481690 4.0478363 107.155685 45-49
TGAGG 14743065 3.8639226 70.48288 35-39
AGGAG 12398795 3.8380234 97.02019 35-39
AGATA 7468330 3.8325527 21.05717 150-159
TACAC 8340880 3.816172 27.654114 110-119
CCCTT 12329060 3.812224 37.531574 20-24
ACGGT 12906055 3.7780344 98.144775 40-44
ATGGT 12124845 3.7647955 26.638191 190-199
GACGG 15157350 3.7451668 109.885155 40-44
CTGAG 12719270 3.7233562 106.618935 35-39
CCACT 10074870 3.6793861 29.341946 240-247
CAGGG 14884575 3.677768 35.391823 50-59
TGTCC 13189495 3.6512775 20.951088 120-129
GAGGA 11724530 3.629306 127.4948 35-39
TGCAC 10947925 3.5796118 115.810455 25-29
GCCCC 14211215 3.496732 73.384315 60-69
TGGTG 15566785 3.454237 18.400389 190-199
CTTGG 13880955 3.4403632 21.50895 20-24
CCTGA 10484430 3.4280648 84.90298 30-34
CCGTG 14663115 3.4262452 23.766012 120-129
GGGTT 15430300 3.4239514 41.816147 50-59
AGGCT 11673400 3.4171953 26.999617 30-34
CTCTC 11029020 3.4102433 14.435317 130-139
TCTTG 11574390 3.3986585 18.83546 170-179
GGATG 12866850 3.3721967 70.255806 20-24
CAGAT 8175060 3.348693 18.859102 150-159
TCAGG 11344340 3.3208683 21.56737 140-149
GCACT 10144000 3.3167548 89.53185 25-29
CCAGG 11972690 3.3042383 40.09162 50-59
CTTCA 8518045 3.2996557 18.114304 200-209
TCGCA 9962750 3.2574918 24.51954 100-109
TCTCA 8350835 3.234883 22.584496 130-139
TGTCT 10902330 3.2013175 20.489305 180-189
CGCAC 10262570 3.1634972 24.921162 100-109
CCTGG 13397685 3.1305594 29.083378 50-59
CGGAT 10543045 3.0863023 79.76283 20-24
ACAGA 6345875 3.0701768 14.452927 140-149
GGTTG 13659195 3.030947 73.61379 15-19
TTCCC 9669715 2.9899375 49.061993 50-59
GATGC 10153895 2.9723854 96.516716 25-29
TGGCC 12601565 2.9445345 43.551914 60-69
ACCAC 6802580 2.9342492 20.975895 230-239
GCCCT 11234590 2.932116 20.075909 20-24
TCTCT 8932625 2.9296808 15.919499 180-189
GGTTC 11795320 2.9234433 47.12677 50-59
AGGGT 11066455 2.900342 49.880833 50-59
TCAAA 4993410 2.8621576 58.531765 70-79
TTCTT 8175045 2.843964 21.51523 170-179
GATGG 10822555 2.8364196 20.80842 15-19
GTTGG 12725580 2.8237793 52.968006 15-19
CGGTG 13443355 2.8123417 68.88043 40-44
GACTC 8592535 2.8094766 19.310276 190-199
CGGCC 12656665 2.7881663 12.78991 120-129
GCCGT 11899265 2.7804322 22.43673 120-129
AGATG 7416610 2.7199323 18.094315 180-189
AGTCA 6581750 2.6960366 31.656704 70-79
CCAGT 8180220 2.6746633 35.690918 60-69
GTTCC 9652295 2.6720665 53.275288 50-59
TCACA 5825910 2.665507 11.657643 140-149
TGTTC 8977475 2.6361105 13.768741 140-149
TCCAT 6742175 2.6117322 19.713377 70-79
TGGAG 9957840 2.6097913 18.315287 25-29
ACAGC 6715905 2.593559 11.727124 120-129
TCCTC 8286215 2.5621505 17.565594 130-139
GGCTG 12224960 2.5574543 13.965901 30-34
GCGGA 10316495 2.5490599 87.433495 20-24
AGGGA 8147945 2.522181 9.057585 150-159
GAGAT 6807355 2.496497 17.654171 180-189
GTTCT 8354740 2.4532533 18.328613 160-169
GGCGG 13743615 2.4268162 42.033825 20-24
CATTT 5877220 2.4148679 18.94524 150-159
CTGGA 8186090 2.3963428 15.543634 180-189
AATAA 3335310 2.39504 10.236192 110-119
TACCA 5234185 2.3947773 17.415575 230-239
ACTCT 6161485 2.386789 13.567032 200-209
TCATT 5798345 2.3824592 17.560793 150-159
TTGGG 10730530 2.381082 79.64598 15-19
TTCAT 5750065 2.3626215 17.127172 140-149
CACGG 8550600 2.3598056 9.554874 120-129
CAGCT 7129060 2.3309684 12.117581 150-159
CTGGC 9941260 2.3229167 34.374 60-69
GTGAA 6333200 2.3226075 19.274399 190-199
GATAC 5649245 2.314061 15.770274 150-159
ACTAC 4989595 2.282871 14.167931 230-239
TGTAG 7328890 2.275639 8.048945 210-219
ATTTG 6125395 2.2533247 16.713835 150-159
TTCAC 5804025 2.248319 19.87149 200-209
CTACT 5782450 2.2399614 8.536439 230-239
TTGTC 7621750 2.238021 13.889329 170-179
CAGAG 6452590 2.230971 9.351838 140-149
TACAG 5328430 2.1826475 15.011774 160-169
CACTA 4763395 2.1793787 16.627867 240-247
TCAGC 6541940 2.1389995 14.032752 140-149
GAATC 5205905 2.1324587 15.287208 190-199
CTCGC 8126680 2.1209824 16.428953 100-109
ATGTA 4879775 2.1202004 8.842444 70-79
GTTCA 6072470 2.106019 15.855924 140-149
ACACA 3887915 2.1009722 14.483097 110-119
ATATA 3454425 2.1002204 11.414496 110-119
GTCTC 7580950 2.0986514 12.593084 180-189
TGGGG 11200720 2.097851 52.338783 15-19
TTGGA 6756125 2.0977938 16.271042 170-179
GTCCT 7564155 2.094002 14.89148 130-139
CTCTG 7559040 2.092586 15.257784 180-189
TGAAT 4808635 2.0892909 14.808143 190-199
GCTGT 8418090 2.0864046 10.726203 140-149
TAGTC 6010885 2.0846603 26.174665 70-79
CAGGC 7522935 2.0761893 15.906892 140-149
TCTGC 7446670 2.0614784 12.763012 210-219
AATAT 3385815 2.058507 14.039197 110-119
TTGCA 5934285 2.0580945 15.101159 150-159
TCTCG 7384570 2.0442872 15.751648 100-109
AATCG 4987245 2.0428905 14.001627 190-199
AGCTC 6227005 2.036026 12.129576 140-149
TTTGC 6907760 2.0283675 12.7985525 150-159
GTCAA 4908305 2.0105548 40.23893 70-79
GAACT 4889010 2.0026512 10.8938875 160-169
ACGGC 7226390 1.9943484 14.674087 120-129
AACCA 3688720 1.9933301 15.049411 70-79
TGGAA 5414360 1.9856366 9.89989 170-179
CCTTC 6414740 1.9834787 14.894905 200-209
ATCTC 5104870 1.9774855 7.813389 100-109
TCGGC 8451730 1.9748667 8.43177 130-139
TACTA 4057945 1.9693143 11.307883 230-239
GATAT 4532025 1.9691076 8.303507 180-189
GAGTC 6715970 1.9659895 13.261988 210-219
CAGCC 6304880 1.9435164 10.585626 120-129
ATATG 4460505 1.9380329 8.56958 180-189
CCAGA 4977290 1.922138 29.455153 60-69
ACACG 4962445 1.916405 13.439188 120-129
AGTAT 4362260 1.8953469 13.438287 220-229
GACGT 6421060 1.8796593 9.619132 170-179
GGGGC 10596320 1.871074 47.583553 15-19
CATAG 4559700 1.867758 8.565512 210-219
CTGTT 6348055 1.8640181 13.52966 140-149
GGGCG 10341945 1.826157 58.615303 20-24
GCAGA 5273895 1.8234394 15.381828 150-159
CGTAG 6177135 1.8082542 10.295898 210-219
ATTGT 4872895 1.7925725 8.156245 50-59
GTTGT 6816890 1.7921071 7.0076084 210-219
AGAAC 3691520 1.785982 11.564028 160-169
AGTCT 5081880 1.7624685 14.153114 210-219
CCTTG 6361985 1.7612029 24.443972 20-24
TGCAG 6015295 1.7608783 13.999782 150-159
ATAGT 3857065 1.6758459 7.6888504 220-229
GGTCA 5715660 1.6731651 7.5785527 130-139
CAAAA 2464870 1.6686983 22.194052 70-79
AACTG 4071180 1.6676491 10.752533 160-169
CACCA 3852800 1.6618805 13.874504 230-239
GTCCC 6363100 1.6607057 21.914831 50-59
GTCAC 5074955 1.6593436 8.4962 130-139
GAGAA 3815655 1.6527578 10.672684 160-169
TGACT 4726075 1.63907 11.617449 190-199
TGGTT 6229770 1.637758 18.118816 10-14
TCTGG 6594530 1.6344393 12.555905 180-189
CTCCA 4472950 1.6335405 7.4734325 150-159
GAACC 4221490 1.6302619 12.401261 70-79
TCCCA 4438360 1.6209081 10.932245 240-247
AATAG 3146410 1.6146557 10.40922 240-247
TTGGT 6113640 1.6072283 25.704838 25-29
TCAGA 3903695 1.5990434 9.930887 130-139
ACTGA 3900980 1.5979311 8.835839 180-189
GTCTG 6409890 1.5886767 9.705774 210-219
TTCAG 4521240 1.5680304 9.300699 150-159
CAAAG 3224435 1.5600029 28.874474 70-79
GGCCG 7906365 1.5593532 13.088912 120-129
TTGTA 4220210 1.5524719 9.0193405 210-219
ACCAT 3392160 1.5520027 12.479792 45-49
GCTCT 5602090 1.5508392 10.637072 130-139
GCTCC 5937705 1.5496818 6.0197163 150-159
CCTCG 5923275 1.5459158 10.206236 130-139
TGTGC 6230115 1.5441197 8.408237 220-229
CAGAC 3995755 1.5430871 17.80751 60-69
ATAGA 2998115 1.5385544 10.413757 240-247
GTGCT 6204915 1.537874 8.409809 220-229
TAGTT 4179220 1.5373931 9.907186 220-229
GCTCA 4695700 1.5353396 10.861833 140-149
CTGGT 6193845 1.5351304 6.8907156 160-169
TGCTC 5484505 1.5182879 5.12254 150-159
GGACG 6137040 1.5163758 7.0947056 170-179
CTGTG 6102355 1.5124549 5.172622 210-219
CCACC 4358745 1.5007371 10.189752 230-239
CCCAC 4305630 1.4824493 10.887449 240-247
GCTGA 5023570 1.4705671 22.952206 30-34
AATTG 3380380 1.4687322 10.061977 170-179
TATAC 2976620 1.444549 7.290076 110-119
AAATA 2006680 1.4409692 5.1545243 9
CATGT 4145215 1.4376194 8.687514 70-79
CGACT 4344400 1.4204761 8.566759 190-199
ACCGT 4314820 1.4108044 21.08113 45-49
TGATA 3242930 1.409012 7.8030562 180-189
TCGAC 4253325 1.3906976 9.2488785 190-199
GCGTA 4722250 1.3823607 10.943156 210-219
ACGGA 3993810 1.3808523 10.109124 200-209
GAATT 3167775 1.3763583 11.071939 170-179
CTCTT 4170735 1.3678982 8.122622 200-209
CACTG 4177145 1.3657892 7.3898935 230-239
TGGAC 4650660 1.361404 10.076326 170-179
GTGGA 5128805 1.3441782 23.120756 25-29
GGGAG 6066345 1.3419713 6.4762344 150-159
CCTCA 3649510 1.3328168 7.256277 130-139
GTCCA 4060200 1.327552 18.34328 70-79
TATGG 4257000 1.3218094 6.195148 180-189
AGCCG 4785720 1.3207692 9.435018 120-129
CTCGA 4021840 1.3150096 8.293297 190-199
GAGGG 5938120 1.3136059 8.117089 200-209
CCATC 3590370 1.3112187 10.956865 240-247
GTGTT 4969870 1.3065401 8.05432 160-169
CCATG 3991325 1.3050324 8.750006 70-79
GGCTC 5573105 1.3022351 8.895683 130-139
CTGAT 3733830 1.2949454 7.353202 180-189
GTAGG 4928800 1.29176 5.0006514 160-169
ACTTC 3321440 1.2866341 6.8714085 220-229
GGAGT 4892420 1.2822254 7.610471 210-219
TTGGC 5150480 1.2765347 8.802415 60-69
ATCGG 4359115 1.2760589 8.374009 190-199
CTGCT 4608210 1.2757012 5.683178 90-99
AGATC 3083990 1.2632734 6.757971 130-139
ACGTG 4313405 1.262678 9.192975 170-179
AAGTA 2457685 1.26122 16.061869 70-79
GGAAT 3438850 1.2611475 9.262134 170-179
CTTGA 3621755 1.2560761 8.370704 200-209
GGAGG 5666465 1.2535115 17.325968 30-34
ATAAC 2185885 1.2529209 7.6338735 80-89
TCCAC 3428440 1.252081 9.662978 240-247
ACTAT 2544140 1.2346671 6.516981 230-239
GTGGT 5563595 1.2345501 11.931354 50-59
TGGTC 4955340 1.2281698 13.496778 50-59
AAAGT 2379375 1.2210333 21.28428 70-79
ATAAA 1694345 1.2166858 5.917665 7
ACTCG 3717735 1.2155772 8.501333 190-199
AAAAA 1417255 1.2020209 8.910334 8
GATCT 3459390 1.1997658 5.315576 130-139
ACTGG 4077625 1.1936574 7.126299 160-169
CCATA 2593050 1.1863889 11.580568 70-79
ATACC 2578580 1.1797683 8.435966 70-79
TCACG 3606740 1.1792855 10.199594 200-209
TTGAG 3791210 1.1771804 8.078663 200-209
AGCTT 3380330 1.1723465 6.275368 150-159
TGCCC 4491235 1.1721675 7.4184966 60-69
AGTTC 3312770 1.1489158 5.739106 160-169
GCATA 2804735 1.148884 7.969546 210-219
TTCCA 2951760 1.1434304 7.7701902 230-239
CGGCT 4868610 1.1376199 8.36302 130-139
CTCGG 4855825 1.1346325 8.800398 130-139
TATCA 2333135 1.1322668 7.28367 70-79
AGAGC 3255330 1.1255243 7.0677414 140-149
AGGTT 3602635 1.1186274 23.516834 10-14
AAAAG 1841815 1.116344 15.126983 70-79
ACTGT 3201170 1.1102113 5.569286 230-239
TCTAC 2863415 1.1092079 7.3660645 180-189
CAATA 1929110 1.1057408 6.6399264 100-109
GTCTA 3174050 1.1008056 7.490581 180-189
TATGT 2961630 1.089483 8.36325 220-229
ATGGG 4149665 1.0875611 32.833763 15-19
GAAGA 2510320 1.0873495 18.761734 35-39
CTACC 2919775 1.0663143 9.299946 230-239
TACTG 3073680 1.0659959 6.48836 180-189
GAGCT 3639815 1.0654958 6.3001676 140-149
AGAGA 2455515 1.0636107 14.085015 35-39
TCTGT 3614490 1.0613447 6.3125405 210-219
GCTTC 3825025 1.0588902 5.5299892 150-159
CTGCG 4529365 1.0583504 7.460526 210-219
ATATC 2174505 1.0552838 7.713393 70-79
ATACG 2559895 1.048592 5.678176 120-129
ATCCA 2284675 1.0452989 15.546783 240-247
CTGAA 2485000 1.0179132 15.357195 35-39
TGCGT 4080320 1.0112981 8.704208 210-219
AACTT 2070975 1.005041 5.823932 210-219
GCGGT 4783860 1.0007806 5.6444063 130-139
CTGCC 3833325 1.0004596 5.7103295 200-209
CAGCG 3598975 0.9932498 6.76977 160-169
TATAA 1630690 0.99142647 6.8846765 240-247
CGAAC 2561725 0.98929113 12.982396 70-79
CATGG 3362930 0.98444223 5.239482 190-199
TCCCC 3369885 0.9823602 5.346135 80-89
AAAAT 1366440 0.98122174 5.505587 8
CGGAG 3954030 0.97698486 7.0949383 210-219
TAGAT 2235870 0.9714572 7.955417 240-247
GGGGT 5183720 0.97089046 7.539255 10-14
TGCTA 2774685 0.9623002 5.1004324 230-239
GAGTT 3080460 0.95649076 5.5053697 160-169
AGCTG 3260565 0.95447654 5.099813 90-99
ATGTG 3073480 0.9543234 5.2087464 220-229
CGTGG 4552100 0.9522966 8.213618 50-59
ACAAT 1650170 0.945856 7.6469154 100-109
ACCCA 2190045 0.94466186 13.502927 240-247
CCATT 2436980 0.9440189 6.1363745 50-59
ACTAA 1641285 0.9407632 13.797866 240-247
TGAAG 2558130 0.9381564 10.237496 35-39
ACGTC 2826595 0.9242037 12.524778 70-79
AAGAG 2128525 0.9219744 14.005602 35-39
CTTCT 2806205 0.92036605 5.325241 210-219
CCCAA 2109220 0.90979856 9.95572 240-247
TAGGC 3100405 0.90759236 5.5930896 160-169
TGTAA 2083690 0.9053369 6.9017696 230-239
TCGAA 2203195 0.9024794 16.695257 70-79
GAGGC 3560750 0.8798109 14.781266 30-34
AGCGT 2992080 0.875882 6.9910197 160-169
TGCAT 2516020 0.8725915 6.1091833 210-219
GGTGG 4649370 0.8708088 22.755142 25-29
ATCAA 1509180 0.8650423 13.761095 70-79
TGAAC 2092760 0.85724264 7.0076137 190-199
CATCA 1864920 0.85324997 5.810481 230-239
CCAAT 1841125 0.8423633 10.811508 240-247
CGGTC 3589235 0.83867574 5.4345627 130-139
CGTCC 3198775 0.83484834 10.19137 70-79
GGGCC 4216260 0.83156276 17.214714 15-19
CCAGC 2691190 0.8295751 5.6834583 80-89
AACCG 2137190 0.82534343 6.2301836 190-199
GTATG 2644760 0.8212048 5.811265 220-229
GACCG 2942410 0.8120501 19.252882 45-49
TTCTG 2747685 0.8068195 5.2796245 210-219
GGTCC 3404965 0.7956184 13.559567 50-59
CTTCC 2572325 0.7953793 5.5152054 230-239
TAACC 1735350 0.7939684 6.123265 80-89
TGGGC 3752995 0.78512424 18.71468 15-19
AAAGG 1746945 0.75669235 9.012371 10-14
CTAAT 1547150 0.75082946 10.545775 240-247
AGCCA 1926580 0.7440098 5.9374905 80-89
CATAC 1625220 0.7435809 10.028126 70-79
AAAGA 1210055 0.7334275 5.830226 8
TGAGA 1995630 0.73186785 6.269276 240-247
AATCC 1559700 0.71360385 9.557663 240-247
GTCGA 2437575 0.7135598 11.650358 70-79
GTAGC 2433865 0.7124738 5.6275463 80-89
CCTTT 2137565 0.7010687 5.1280828 50-59
AAGGT 1908050 0.6997492 10.051465 10-14
AAAGC 1443245 0.6982516 10.278023 70-79
ATGAT 1602750 0.69637454 6.8700504 240-247
TCCAG 2054130 0.671633 5.037274 240-247
CATTG 1936265 0.6715242 5.6303678 50-59
CGATG 2282625 0.6682008 14.445831 15-19
TAGGT 2095680 0.65071404 5.475771 10-14
CCGTA 1955365 0.6393402 5.399394 80-89
AGGAT 1734705 0.6361774 6.1807127 10-14
TATGA 1456625 0.63288516 5.9433823 240-247
GAGGT 2356965 0.61772305 6.8618207 10-14
CAATC 1344870 0.6153135 9.255881 240-247
GGAAG 1984195 0.6142037 6.2232633 30-34
AGAAG 1413010 0.6120478 5.619698 9
ATAGG 1647815 0.6043118 5.3623624 10-14
AGACC 1548520 0.59800994 5.3936954 240-247
AATGG 1565855 0.5742542 6.717816 10-14
CATCC 1494770 0.5458964 7.2076244 240-247
TAAAA 758705 0.5448156 5.844326 8
GGGTC 2590525 0.5419362 5.5504827 70-79
AAATG 1049365 0.5385068 5.4471507 9
AAGCA 1101475 0.532901 6.9140716 70-79
AAGAA 868325 0.5263012 5.421074 9
GAAGG 1653360 0.51179445 5.525026 10-14
AAGGG 1642260 0.5083585 6.2872295 10-14
GAAAA 788770 0.4780821 5.9048133 8
ACGAT 1167050 0.47805056 6.6797953 10-14
GGTCG 2278720 0.47670683 5.6264997 70-79
TTGCC 1708535 0.47297758 5.2907305 60-69
ATAAG 917645 0.47091153 5.7987394 9
TGATC 1352625 0.4691096 5.183378 240-247
GATCC 1372575 0.44878703 5.2148037 240-247
TACCG 1345830 0.4400422 5.3096495 70-79
TAAGG 1177940 0.4319921 5.98422 10-14
CGGTT 1740675 0.43142235 7.996638 10-14
GAAGC 1217530 0.42095873 5.120034 30-34
AAGCT 999370 0.40936497 8.581847 30-34
TAAAG 782330 0.4014714 5.714556 9
GAAAG 853095 0.36951962 5.190227 9