FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_8_SL248354.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_8_SL248354.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10183034
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11602505 8.938691 62.668777 110-119
ATACA 10673540 7.7517414 37.9714 110-119
CACAG 15515300 7.596745 42.460617 110-119
TCCCT 18103865 7.1261196 104.17233 30-34
CAGTA 13020805 6.7629557 39.58038 110-119
AGTAA 10121540 6.5759535 48.319347 110-119
GGAGA 16161635 6.332794 95.83611 35-39
GTAAT 10940525 6.0279303 40.19462 110-119
GGTGA 17949255 5.9645057 102.724594 45-49
CCCTG 17792530 5.9062185 68.07575 30-34
AATAC 7681705 5.578898 39.99623 110-119
AGACG 12598405 5.518287 103.21867 40-44
GTAGT 13632340 5.371659 30.856739 80-89
ACAGT 10322370 5.3613987 36.073154 110-119
GTGAC 13782530 5.1195955 85.74332 45-49
GAGAC 11180460 4.8972063 142.97629 40-44
CCCCA 11152715 4.879925 102.37107 60-69
TAGTA 8680280 4.782597 39.947746 80-89
CTCAG 11399830 4.7335134 34.97826 140-149
ACTCC 10023360 4.652402 117.672874 30-34
ACCAG 9286150 4.546771 92.698 45-49
GGCCC 16220540 4.5407457 58.870453 60-69
GCACA 9088645 4.450066 34.457043 110-119
TGACC 10633255 4.4152117 147.08537 45-49
GTGTC 13952380 4.395142 24.034813 120-129
AGTAG 9414305 4.3742976 32.12707 80-89
CGTGT 13878490 4.3718657 23.546562 120-129
CACTC 9241465 4.289481 96.40556 25-29
CTCCC 11142570 4.1346216 67.49857 30-34
CCCAG 10414535 4.0765595 87.43246 60-69
GACCA 8238200 4.0336637 106.12643 45-49
ATGCA 7752520 4.026629 94.63797 25-29
TGAGG 11545805 3.8366506 69.77779 35-39
CCCTT 9706480 3.8207061 39.89294 20-24
AGGAG 9742930 3.8176816 96.17171 35-39
TACAC 6502300 3.775238 27.367773 110-119
ACGGT 10150815 3.7705748 97.58581 40-44
GACGG 11961710 3.7470536 109.25056 40-44
AGATA 5733835 3.7252662 20.347368 150-159
ATGGT 9448635 3.7231205 26.280727 190-199
CTGAG 9986615 3.7095819 105.37935 35-39
TGTCC 10423040 3.6702662 20.902334 120-129
CAGGG 11630495 3.6432993 34.756256 50-59
CCACT 7780215 3.6112332 28.468454 240-247
GAGGA 9208075 3.6081035 126.4274 35-39
TGCAC 8503885 3.5310404 113.130394 25-29
GCCCC 11221675 3.5115442 73.214035 60-69
TGGTG 12298460 3.4657457 18.140963 190-199
AGGCT 9309950 3.4582314 28.635761 30-34
CTTGG 10950435 3.4494984 22.780233 20-24
CCGTG 11511355 3.4183745 23.55027 120-129
GGGTT 11981265 3.3763595 41.095154 50-59
CCTGA 8116410 3.3701499 82.938354 30-34
GGATG 10077020 3.3485758 68.577644 20-24
CTCTC 8471065 3.334416 13.9789505 130-139
CAGAT 6395715 3.3219094 18.39701 150-159
TCTTG 8885795 3.3191805 18.28309 170-179
CCAGG 9413630 3.2963428 39.49962 50-59
TCAGG 8865165 3.293013 21.095451 140-149
GCACT 7836185 3.253793 87.50385 25-29
CTTCA 6569725 3.2347627 17.456127 200-209
TCGCA 7721425 3.2061417 24.231388 100-109
TCTCA 6473450 3.1873593 22.265537 130-139
CCTGG 10647510 3.16185 28.93047 50-59
TGTCT 8428270 3.1482778 19.899527 180-189
CGCAC 7959265 3.1154933 24.700993 100-109
ACAGA 5017505 3.0730426 14.452598 140-149
CGGAT 8155130 3.0292668 77.90262 20-24
GGTTG 10582900 2.9822955 71.746635 15-19
TTCCC 7548455 2.9712543 48.656162 50-59
GCCCT 8913415 2.9588022 21.205631 20-24
TGGCC 9943635 2.9528298 43.16011 60-69
GATGC 7846380 2.9145803 93.971504 25-29
TCTCT 6936325 2.8962939 15.535233 180-189
GGTTC 9182470 2.8925717 46.41566 50-59
AGGGT 8649595 2.8742452 48.950512 50-59
GATGG 8646020 2.8730571 21.883396 15-19
TCAAA 3895280 2.828977 57.53688 70-79
ACCAC 5159735 2.8240564 19.718801 230-239
CGGTG 10622180 2.821813 68.467255 40-44
GACTC 6775045 2.8131797 19.282019 190-199
GCCGT 9330880 2.7708678 22.23489 120-129
GTTGG 9817160 2.7665071 51.76332 15-19
TTCTT 6222575 2.756225 20.695604 170-179
CGGCC 9782900 2.7386055 12.516776 120-129
TCACA 4703680 2.7309585 11.645705 140-149
AGATG 5791500 2.6909845 17.8661 180-189
AGTCA 5159570 2.6798606 30.715714 70-79
TCCAT 5420505 2.6689165 19.791252 70-79
GTTCC 7571290 2.6660793 52.55555 50-59
CCAGT 6376220 2.6475766 35.27491 60-69
TGGAG 7861910 2.6124988 19.397646 25-29
GGCTG 9810775 2.606261 14.80737 30-34
TGTTC 6834050 2.5527763 13.394905 140-149
TCCTC 6479040 2.5503068 17.231049 130-139
ACAGC 5094600 2.4944654 11.482822 120-129
AGGGA 6364370 2.4938226 9.041719 150-159
GCGGA 7957520 2.4927254 85.23132 20-24
GAGAT 5314725 2.4694538 17.384022 180-189
AATAA 2685985 2.440101 10.602787 110-119
ACTCT 4883565 2.4045413 13.71872 200-209
GGCGG 10696900 2.3964179 40.992542 20-24
GTTCT 6349980 2.3719578 17.797104 160-169
CTGGA 6377320 2.3688898 15.096458 180-189
CATTT 4506140 2.3535926 18.32689 150-159
TTGGG 8293350 2.3370926 77.76135 15-19
CACGG 6672515 2.3364947 9.537851 120-129
TCATT 4463760 2.3314574 16.961927 150-159
CTGGC 7821320 2.3225937 33.961655 60-69
CAGCT 5566040 2.3111682 11.962228 150-159
TTCAT 4409850 2.3032997 16.670109 140-149
TACCA 3950195 2.2934847 16.366758 230-239
TGTAG 5803490 2.2867951 7.9918714 210-219
GTGAA 4861450 2.2588425 18.607265 190-199
GATAC 4287170 2.2267394 15.143449 150-159
CAGAG 5078690 2.2245412 9.291088 140-149
TTGTC 5920360 2.2114785 13.6416445 170-179
TTCAC 4480665 2.2061636 19.323645 200-209
ATTTG 4689700 2.191256 16.093939 150-159
GAATC 4211640 2.1875095 15.179434 190-199
ACTAC 3706465 2.1519752 12.884908 230-239
TCAGC 5179705 2.1507516 14.081308 140-149
TGAAT 3880905 2.1382725 14.691176 190-199
CTACT 4332725 2.1333218 7.8693705 230-239
CTCGC 6363405 2.1123278 16.41616 100-109
TACAG 4063115 2.110366 14.087749 160-169
CACTA 3610745 2.0964003 15.526898 240-247
ATGTA 3804160 2.0959883 8.809697 70-79
GTCCT 5921425 2.0851123 14.558715 130-139
GCTGT 6606365 2.081072 10.375549 140-149
CTCTG 5899100 2.0772507 14.845005 180-189
GTCTC 5883445 2.0717385 12.167446 180-189
TTGGA 5244810 2.066654 15.7488 170-179
GTTCA 4681970 2.0622687 15.508311 140-149
CAGGC 5885505 2.0609097 15.527412 140-149
ATATA 2674820 2.0607092 10.938475 110-119
TCTGC 5842190 2.057211 12.367008 210-219
TAGTC 4666925 2.055642 25.294996 70-79
TGGGG 8641765 2.053707 51.0785 15-19
TCTCG 5827705 2.0521104 15.971743 100-109
ACACA 2992280 2.0486212 14.104235 110-119
ATCTC 4138270 2.0375772 8.092191 100-109
TTGCA 4599385 2.0258925 14.517076 150-159
AATCG 3892530 2.0217652 13.829706 190-199
AATAT 2621205 2.0194037 13.603429 110-119
GAACT 3883860 2.0172617 10.81882 160-169
AGCTC 4849735 2.0137396 12.165807 140-149
TTTGC 5352400 1.9993238 12.305453 150-159
ACGGC 5670915 1.9857675 14.390111 120-129
GTCAA 3823130 1.9857188 39.430225 70-79
TGGAA 4262255 1.9804302 9.330167 170-179
GATAT 3555240 1.9588401 8.196492 180-189
AACCA 2858200 1.9568251 14.794265 70-79
CCAGA 3975230 1.9463888 29.695883 60-69
CCTTC 4926705 1.9392704 14.314403 200-209
ACACG 3958790 1.9383394 13.338613 120-129
GAGTC 5202225 1.9323946 12.709294 210-219
TCGGC 6461405 1.9187578 7.883874 130-139
CAGCC 4897085 1.9168651 10.387219 120-129
ATATG 3441960 1.896426 8.423572 180-189
GACGT 5059375 1.8793321 9.635788 170-179
TACTA 3047030 1.8766593 10.518585 230-239
CATAG 3600485 1.870078 8.574155 210-219
CCTTG 5239530 1.8449965 25.916697 20-24
CTGTT 4908750 1.8336039 13.054817 140-149
GGGGC 8175885 1.8316369 46.474255 15-19
AGTAT 3278530 1.8063805 12.43186 220-229
GGGCG 7986145 1.7891296 57.072792 20-24
GTTGT 5329310 1.7808481 7.067588 210-219
GCAGA 4052290 1.7749627 14.789356 150-159
CGTAG 4758455 1.7675537 10.034694 210-219
AGAAC 2871465 1.7586697 11.4892025 160-169
ATTGT 3744975 1.7498345 8.199877 50-59
TGCAG 4693065 1.7432644 13.691358 150-159
AGTCT 3885255 1.7113396 13.457475 210-219
GGTCA 4567630 1.6966709 7.5713487 130-139
GTCCC 5110675 1.6964854 22.190142 50-59
CTCCA 3624515 1.68234 7.6383176 150-159
ATAGT 3045070 1.6777505 7.6230135 220-229
GTCAC 4035955 1.6758364 8.41814 130-139
TCAGA 3209660 1.6670849 10.231416 130-139
CAAAA 1944970 1.6656576 22.079206 70-79
CACCA 3037225 1.6623517 13.672008 230-239
TGACT 3748045 1.6509026 11.622208 190-199
TTGGT 4933715 1.6486557 27.25772 25-29
AACTG 3168505 1.6457092 10.710708 160-169
TCCCA 3513280 1.6307096 11.165412 240-247
GAGAA 2966755 1.6254884 10.605192 160-169
AATAG 2501100 1.624962 9.825548 240-247
TGGTT 4851710 1.621253 17.639671 10-14
TCTGG 5117020 1.6119133 12.142153 180-189
TTCAG 3623700 1.5961323 9.3346 150-159
CAGAC 3234145 1.5835321 17.951107 60-69
CTGTG 5011415 1.5786468 5.3487387 210-219
GAACC 3216925 1.5751007 12.167915 70-79
ATAGA 2420975 1.5729048 10.418304 240-247
GTCTG 4987525 1.5711213 9.232824 210-219
GCTCC 4718410 1.566273 6.1506286 150-159
ACCAT 2689175 1.5613362 12.547385 45-49
GGCCG 6210105 1.5551854 12.942784 120-129
ACTGA 2987855 1.5518802 8.628578 180-189
TGTGC 4921225 1.550236 8.25333 220-229
TGCTC 4386290 1.5445449 5.310706 150-159
CTGGT 4898470 1.5430679 6.8938937 160-169
GCTGA 4153460 1.542825 24.351515 30-34
CAAAG 2505245 1.534373 28.16283 70-79
TTGTA 3281750 1.5333931 9.029704 210-219
GCTCA 3679275 1.5277331 10.892202 140-149
GGACG 4844500 1.517559 7.12521 170-179
TAGTT 3246750 1.5170394 9.749905 220-229
GTGCT 4804645 1.5135123 8.063858 220-229
CCTCG 4554675 1.5119214 9.756913 130-139
GCTCT 4215275 1.4843253 10.082795 130-139
CCACC 3386545 1.4817994 9.763318 230-239
CCCAC 3384840 1.4810532 10.720688 240-247
CATGT 3358240 1.479205 8.763418 70-79
AAATA 1609500 1.4621611 5.101941 9
ACCGT 3421035 1.4205049 21.192644 45-49
CGACT 3420455 1.4202641 8.529765 190-199
GTGGA 4244550 1.4104564 24.42293 25-29
AATTG 2558630 1.4097352 9.481033 170-179
TATAC 2271300 1.3988888 6.9055777 110-119
TCGAC 3361730 1.39588 9.265568 190-199
CCTCA 2985455 1.3857166 7.4209275 130-139
TGATA 2501630 1.3783298 7.6406384 180-189
TGGAC 3706180 1.3766806 10.203364 170-179
CACTG 3301190 1.3707423 7.335247 230-239
CTCTT 3281685 1.3702824 8.104973 200-209
AAAAA 1275980 1.3668793 8.108999 8
GTCCA 3250830 1.3498315 18.445545 70-79
CCATG 3250555 1.3497173 8.8825 70-79
GCGTA 3633055 1.349518 10.673255 210-219
ACGGA 3052665 1.3371122 9.778656 200-209
CCATC 2877460 1.3355902 11.344016 240-247
GGGAG 4754505 1.3323655 6.4343843 150-159
GTAGG 3980310 1.32265 5.1139364 160-169
GAATT 2393510 1.3187587 10.454117 170-179
AGATC 2533445 1.3158615 6.9347258 130-139
CTTGA 2981785 1.3133878 8.352583 200-209
ACTTC 2661055 1.3102348 7.033325 220-229
CTGCT 3718685 1.309461 5.542081 90-99
CTCGA 3146830 1.3066478 8.260047 190-199
AGCCG 3727555 1.3052669 9.275723 120-129
GAGGG 4636925 1.2994158 8.043586 200-209
TATGG 3297645 1.2993971 6.142175 180-189
GGAGG 4598710 1.2887065 18.345848 30-34
GTGTT 3846615 1.2853893 7.8499064 160-169
CTGAT 2914245 1.2836382 7.251478 180-189
ATCGG 3428125 1.2733954 8.265638 190-199
TTGGC 4031510 1.2699667 8.831935 60-69
TCCAC 2732410 1.2682642 9.580819 240-247
TGGTC 4017330 1.2655 13.607651 50-59
GGCTC 4221600 1.2536328 8.39053 130-139
GGAGT 3770495 1.2529289 7.2009206 210-219
GTGGT 4443790 1.2522745 12.023521 50-59
ACGTG 3370715 1.2520703 9.255765 170-179
AAGTA 1924565 1.2503878 15.688685 70-79
GATCT 2789075 1.2285048 5.508804 130-139
ATAAA 1349595 1.2260486 5.6595807 7
ATAAC 1687690 1.225698 7.3759003 80-89
ACTAT 1987660 1.2241956 6.437777 230-239
GGAAT 2623110 1.2188116 8.695851 170-179
AAAGT 1855920 1.2057893 20.609861 70-79
TGCCC 3615300 1.2000966 7.550871 60-69
ACTCG 2888755 1.1994882 8.483699 190-199
CCATA 2025345 1.1759162 11.463805 70-79
GCATA 2263005 1.175396 8.19704 210-219
ACTGG 3152975 1.1711895 7.064598 160-169
TTGAG 2971165 1.1707517 8.052535 200-209
ATACC 2013240 1.1688881 8.3051195 70-79
TATCA 1876205 1.1555507 7.2314463 70-79
TTCCA 2345050 1.154642 7.722308 230-239
CAATA 1586090 1.1519101 6.792973 100-109
AGCTT 2603480 1.1467557 6.0779886 150-159
TCACG 2753625 1.1433785 9.9210825 200-209
AGTTC 2588660 1.1402278 5.608187 160-169
ACTGT 2583925 1.1381422 5.6463056 230-239
ATGGG 3407750 1.1323893 34.649456 15-19
AAAAG 1452540 1.1128135 15.002853 70-79
AGAGC 2533575 1.1097432 6.8352113 140-149
AGGTT 2810120 1.1072938 22.912298 10-14
TCTGT 2958160 1.1049846 6.5341268 210-219
CGGCT 3698430 1.0982739 7.8711495 130-139
CTCGG 3695255 1.0973308 8.317125 130-139
GAAGA 2001480 1.0966132 19.236828 35-39
AGAGA 1976890 1.0831403 14.175018 35-39
GTCTA 2443385 1.0762386 7.338213 180-189
ATATC 1746480 1.0756533 7.68164 70-79
TCTAC 2174755 1.0707932 7.15251 180-189
ATCCA 1808525 1.0500304 15.723001 240-247
CTGAA 2018580 1.0484426 15.467065 35-39
GAGCT 2810615 1.0440181 6.1191497 140-149
TATGT 2232865 1.0433031 7.7228622 220-229
AACTT 1689180 1.0403624 6.1091924 200-209
GCTTC 2938755 1.0348241 5.365729 150-159
TACTG 2347065 1.0338124 6.235652 180-189
GTCCG 3476165 1.0322708 5.0099444 130-139
CTGCC 3101575 1.0295657 5.879825 200-209
CTGCG 3430935 1.0188392 7.0287037 210-219
CATGG 2739945 1.0177673 5.375253 190-199
GCGGT 3807535 1.0114827 5.717447 130-139
ATACG 1942000 1.0086673 5.2004104 120-129
AAAAT 1095835 0.9955187 5.098567 8
TCCCC 2677110 0.99338275 5.2840815 80-89
CTACC 2134920 0.99093574 8.484892 230-239
TAGAT 1789365 0.9858912 8.021527 240-247
TATAA 1276045 0.9830784 6.846934 240-247
CGAAC 1994595 0.97661203 12.761533 70-79
ACAAT 1343720 0.975887 7.7873373 100-109
CGTGG 3662590 0.97297764 8.258914 50-59
TGCGT 3085525 0.97197187 8.42617 210-219
CTTCT 2301870 0.9611562 5.413667 210-219
GGGGT 4031605 0.9581069 7.4171515 10-14
CAGCG 2736035 0.95806926 6.278567 160-169
CCATT 1944240 0.9572936 6.1531854 50-59
ACGTC 2289790 0.950782 12.622743 70-79
TAGGC 2549665 0.9470869 5.733003 160-169
CGGAG 3007295 0.94204724 6.7198615 210-219
GAGTT 2390620 0.9419949 5.344927 160-169
AAGAG 1716020 0.9402092 14.333973 35-39
TGAAG 2013955 0.9357716 10.338131 35-39
GAGGC 2976355 0.9323552 15.671746 30-34
ACCCA 1698590 0.92968225 13.228292 240-247
CCCAA 1663700 0.91058594 9.685443 240-247
GGTGG 3823865 0.9087377 24.117893 25-29
TCGAA 1740265 0.9038869 16.535215 70-79
TGTAA 1626685 0.896259 6.8388257 230-239
TGCAT 2027195 0.89291924 6.238158 210-219
ACTAA 1217960 0.88455284 12.635197 240-247
ATCAA 1216255 0.88331455 13.801013 70-79
CATCA 1513535 0.878759 5.9955587 230-239
GGGCC 3500865 0.87671536 18.220343 15-19
CGTCC 2559015 0.8494633 10.274363 70-79
TTCTG 2269135 0.8476078 5.405524 210-219
AGCGT 2265055 0.8413668 6.4982553 160-169
CCAAT 1445595 0.8393131 10.598841 240-247
CGGTC 2819465 0.83725923 5.4471235 130-139
GACCG 2377135 0.8323943 19.446339 45-49
TGAAC 1598690 0.8303534 6.520434 190-199
TGGGC 3125450 0.83028495 19.79343 15-19
CCAGC 2101310 0.82251537 5.605851 80-89
GGTCC 2737955 0.8130543 13.722353 50-59
GTATG 2012360 0.79294616 5.3560743 220-229
CTTCC 2001270 0.7877483 5.3777947 230-239
TAACC 1356775 0.78774416 5.918514 80-89
AACCG 1592440 0.77970517 5.857364 190-199
TGAGA 1638805 0.76146054 6.0351243 240-247
AAAGG 1363135 0.7468632 8.718128 10-14
CATAC 1278370 0.74222213 9.905218 70-79
AGCCA 1505395 0.7370854 5.888735 80-89
AAAGA 955050 0.7316786 5.6721015 8
GTCGA 1946830 0.72316056 11.586103 70-79
CCTTT 1716710 0.7168201 5.1722236 50-59
CTAAT 1155030 0.71138054 9.789281 240-247
AATCC 1220755 0.70877093 9.377905 240-247
GTAGC 1907670 0.70861435 5.473159 80-89
AAAGC 1142270 0.6995996 10.211447 70-79
ATGAT 1269235 0.6993138 6.899321 240-247
AAGGT 1477025 0.68629044 9.744456 10-14
TCCAG 1641530 0.68160707 5.033677 240-247
TGTTA 1457975 0.6812368 5.011985 230-239
CATTG 1540915 0.67872727 5.6300535 50-59
AGGAT 1438970 0.6686084 6.6625533 10-14
CGATG 1798810 0.66817766 15.104397 15-19
GGAAG 1661080 0.65087956 6.5909514 30-34
TAGGT 1641895 0.6469689 5.3452835 10-14
CCCAT 1386965 0.643768 5.0143437 240-247
CCGTA 1538125 0.6386705 5.412989 80-89
AGAAG 1152760 0.6315984 5.964281 9
GAGGT 1892460 0.62886107 6.7133565 10-14
TATGA 1126765 0.62081677 5.78228 240-247
AGACC 1253210 0.6136083 5.1658716 240-247
ATAGG 1311585 0.6094198 5.193027 10-14
CAATC 1048775 0.6089192 9.078112 240-247
AATGG 1227450 0.570327 6.5204854 10-14
CATCC 1219910 0.56622845 7.5975327 240-247
AAATG 862800 0.5605602 5.2789173 9
TAAAA 610755 0.55484444 5.9281087 8
GGGTC 2058530 0.54685456 5.4816213 70-79
AAGAA 695800 0.5330631 5.442599 8
GAAGG 1352775 0.53007305 5.5691457 10-14
AAGCA 863265 0.52871895 6.836505 70-79
GAAAA 667605 0.5114625 5.9965367 8
AAGGG 1296745 0.50811815 6.270179 10-14
ACGAT 965245 0.5013445 6.938363 10-14
TTGCC 1368495 0.4818883 5.342779 60-69
TGATC 1091040 0.4805707 5.4203515 240-247
GGTCG 1797200 0.47743142 5.5627003 70-79
ATAAG 734120 0.47695696 5.4422474 9
GATCC 1114575 0.46280134 5.441562 240-247
GAAGC 1001895 0.4388448 5.344211 30-34
CGGTT 1393055 0.43882653 7.899622 10-14
TAAGG 934475 0.434198 5.8743052 10-14
TACCG 1045090 0.43394923 5.1942625 70-79
AAGCT 813150 0.42234695 9.02561 30-34
TAAAG 616290 0.40040293 5.561033 9