FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_9_SL248355.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_9_SL248355.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12235244
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13840260 8.849661 61.871075 110-119
ATACA 12672860 7.6551166 37.99513 110-119
CACAG 18508785 7.5640945 42.271034 110-119
TCCCT 21894320 7.2458634 106.92508 30-34
CAGTA 15719910 6.8003974 39.43428 110-119
AGTAA 12081160 6.5092974 48.086132 110-119
GGAGA 19270825 6.2657905 94.28403 35-39
CCCTG 21781075 6.0740876 69.64812 30-34
GTAAT 13149170 6.0212655 39.908985 110-119
GGTGA 21411180 5.9167085 101.329994 45-49
AATAC 9194960 5.5542707 39.9926 110-119
AGACG 14754840 5.378508 102.044914 40-44
GTAGT 16221160 5.319593 29.03247 80-89
ACAGT 12225930 5.2889094 35.787342 110-119
GTGAC 16479060 5.1053286 84.28431 45-49
GAGAC 13438035 4.8985 141.78172 40-44
CCCCA 13174450 4.8464303 101.72625 60-69
ACTCC 12345450 4.8073044 121.53832 30-34
CTCAG 13660260 4.7446256 35.259468 140-149
ACCAG 11384200 4.652448 93.36726 45-49
TAGTA 10043655 4.599189 37.15314 80-89
GCACA 10953085 4.4762616 34.31892 110-119
GGCCC 18984265 4.4610653 58.962933 60-69
CACTC 11341370 4.416317 98.982956 25-29
TGACC 12642400 4.3910913 144.50923 45-49
GTGTC 16586225 4.367197 23.859016 120-129
CGTGT 16469405 4.3364377 23.28977 120-129
AGTAG 11160565 4.306443 30.018696 80-89
CTCCC 13479575 4.2143407 69.516975 30-34
ATGCA 9554250 4.133147 96.62772 25-29
GACCA 9996125 4.085175 106.12213 45-49
CCCAG 12385355 4.0639305 86.79101 60-69
TGAGG 13850510 3.827413 68.97663 35-39
AGGAG 11696890 3.8031726 94.53636 35-39
TACAC 7761250 3.7641559 27.621225 110-119
ACGGT 12113125 3.7527313 96.65759 40-44
GACGG 14219635 3.7121272 108.002716 40-44
CAGGG 14185975 3.70334 35.429012 50-59
CTGAG 11942590 3.699898 105.110435 35-39
ATGGT 11254415 3.690791 26.148039 190-199
CCCTT 11019435 3.6468508 35.912437 20-24
CCACT 9360665 3.6450324 28.505653 240-247
AGATA 6760895 3.6427524 19.696205 150-159
TGTCC 12300560 3.6310468 20.807447 120-129
TGCAC 10449065 3.6292794 115.90181 25-29
GAGGA 11009765 3.5797577 123.97281 35-39
GCCCC 13229805 3.4853787 72.84785 60-69
CCTGA 9961620 3.4599748 84.49603 30-34
GGGTT 14726220 3.4585562 42.052372 50-59
CAGAT 7903800 3.4191658 18.190573 150-159
TGGTG 14460100 3.3960562 18.091404 190-199
GGATG 12278615 3.39304 69.86225 20-24
AGGCT 10845305 3.3599515 24.972696 30-34
CCAGG 11454330 3.3523998 40.234566 50-59
CCGTG 13460415 3.348178 23.347298 120-129
GCACT 9626480 3.3435705 89.60065 25-29
CTTGG 12643405 3.329042 20.419407 20-24
CTCTC 9997645 3.3086922 13.74603 130-139
TCTTG 10574175 3.3041418 18.185072 170-179
CTTCA 7941345 3.2733614 17.210136 200-209
TCAGG 10525365 3.260832 21.056206 140-149
CCTGG 12930070 3.2162585 29.328716 50-59
TCGCA 9203120 3.1965246 24.17292 100-109
TCTCA 7688700 3.169223 22.265478 130-139
TGTCT 10019185 3.1307223 19.88079 180-189
CGGAT 10017660 3.1035414 79.51559 20-24
CGCAC 9448445 3.1002598 24.656483 100-109
TTCCC 9250715 3.061498 49.854843 50-59
GGTTG 12967620 3.0455368 73.63215 15-19
ACAGA 5954665 3.0309417 14.289569 140-149
GATGC 9661400 2.9931698 96.47185 25-29
TGGCC 11954865 2.973683 43.49607 60-69
GGTTC 11245495 2.9609685 47.5977 50-59
AGGGT 10607240 2.9311767 49.724102 50-59
TCAAA 4772980 2.883147 58.497433 70-79
GCCCT 10295975 2.8712382 19.246191 20-24
TCTCT 8125035 2.8463552 15.375672 180-189
GTTGG 12112270 2.8446517 52.865604 15-19
ACCAC 6194555 2.838186 20.204329 230-239
GACTC 8101905 2.814039 19.700346 190-199
CGGTG 12682235 2.813806 67.989265 40-44
AGTCA 6486460 2.806028 31.83489 70-79
GATGG 10043930 2.775513 19.764317 15-19
CCAGT 7983050 2.7727573 36.505806 60-69
GCCGT 11146680 2.7726536 22.089615 120-129
TTCTT 7418250 2.750873 20.720673 170-179
GTTCC 9184745 2.711278 54.065125 50-59
CGGCC 11500350 2.7024388 12.570304 120-129
TCACA 5451225 2.6438086 11.58606 140-149
AGATG 6847515 2.642199 17.537989 180-189
TCCAT 6363590 2.6230228 18.171637 70-79
GCGGA 9818220 2.5631094 87.02621 20-24
GGCTG 11447490 2.5398533 12.968283 30-34
TCCTC 7655855 2.5336835 17.105616 130-139
TGGAG 9062925 2.5044246 17.04833 25-29
AGGGA 7678885 2.4967425 9.038985 150-159
TGTTC 7986005 2.4954093 13.036284 140-149
ACAGC 6013490 2.4575684 11.290721 120-129
GGCGG 13097780 2.4487202 41.965336 20-24
ACTCT 5858455 2.4148102 13.730383 200-209
GAGAT 6253590 2.4130256 17.002007 180-189
TTGGG 10226065 2.4016633 79.36673 15-19
CTGGC 9532600 2.371163 34.757557 60-69
GTTCT 7579390 2.368353 17.839643 160-169
CAGCT 6794800 2.3600416 12.302153 150-159
AATAA 3125400 2.3513863 10.114775 110-119
TACCA 4827175 2.3411486 16.893612 230-239
CTGGA 7517280 2.3289063 14.795754 180-189
CACGG 7892600 2.3099694 9.551782 120-129
CATTT 5269650 2.2992537 17.923126 150-159
TCATT 5241570 2.2870018 16.669485 150-159
TGTAG 6970735 2.285994 8.086755 210-219
TTCAT 5158395 2.2507107 16.361612 140-149
TCAGC 6416035 2.2284849 14.631923 140-149
CAGAG 6062980 2.2101078 9.384751 140-149
GTGAA 5705725 2.201625 18.232042 190-199
TTCAC 5303885 2.186221 18.864832 200-209
TTGTC 6970345 2.1780431 13.598734 170-179
GATAC 4998640 2.1624005 14.828365 150-159
CTACT 5234110 2.15746 7.6043205 230-239
ACTAC 4440425 2.1535773 12.703164 230-239
ATTTG 5520360 2.148428 15.822861 150-159
TGGGG 10692875 2.1161242 52.09174 15-19
CACTA 4349770 2.10961 15.542718 240-247
CTCGC 7560365 2.1083589 16.424917 100-109
TAGTC 5719240 2.1027446 26.334055 70-79
TCTGC 7059430 2.0838985 12.473028 210-219
ATCTC 5054565 2.083453 8.419121 100-109
GAACT 4814400 2.0826986 10.862006 160-169
TCTCG 7051375 2.081521 16.398552 100-109
ATGTA 4533905 2.0761652 7.972978 70-79
GCTGT 7877115 2.0740652 10.173063 140-149
GAATC 4786755 2.0707395 14.751905 190-199
GTCCT 7007355 2.0685265 14.367263 130-139
TTGGA 6292150 2.0634577 15.732256 170-179
TACAG 4759985 2.0591588 13.806074 160-169
CTCTG 6972695 2.0582952 14.818253 180-189
AGCTC 5906190 2.0514002 12.458378 140-149
GTCTC 6944080 2.049848 12.022165 180-189
ATATA 3198980 2.0454738 10.603142 110-119
ACACA 3581525 2.0438075 13.894759 110-119
GTTCA 5537630 2.0359733 15.226764 140-149
CAGGC 6953630 2.0351558 15.518755 140-149
GTCAA 4704325 2.0350802 40.616795 70-79
TGAAT 4421735 2.0248 14.22972 190-199
AATAT 3140430 2.0080361 13.291353 110-119
GATAT 4364845 1.9987491 8.251454 180-189
AATCG 4615025 1.9964495 13.753011 190-199
TTGCA 5427150 1.9953542 14.504454 150-159
ACACG 4878535 1.9937397 13.319367 120-129
AACCA 3462445 1.975854 15.0298605 70-79
TTTGC 6292955 1.9663771 12.310796 150-159
TGGAA 5074055 1.9578873 9.306946 170-179
ACGGC 6685550 1.9566954 14.299844 120-129
CCTTC 5840625 1.9329383 14.224573 200-209
CAGCC 5817205 1.9087635 10.242247 120-129
GAGTC 6154540 1.9067198 12.624621 210-219
GGGGC 10111300 1.8903772 47.461807 15-19
TCGGC 7592260 1.8885179 7.838023 130-139
ATATG 4122455 1.8877538 8.42169 180-189
CATAG 4329695 1.8730164 8.687771 210-219
TACTA 3645220 1.8713925 10.248248 230-239
CCAGA 4558510 1.8629532 27.368258 60-69
GGGCG 9836810 1.8390594 58.66292 20-24
GACGT 5842060 1.8099113 9.637849 170-179
CTGTT 5740120 1.7936311 12.746492 140-149
TCAGA 4135090 1.7888305 10.501374 130-139
AGTAT 3902175 1.7868834 12.196501 220-229
CTCCA 4549520 1.771578 8.202516 150-159
GTTGT 6285640 1.7519025 7.0422754 210-219
AGAAC 3438145 1.7500257 11.393469 160-169
CGTAG 5646355 1.7492803 9.976314 210-219
GCAGA 4775760 1.7408838 14.5570965 150-159
ATTGT 4460875 1.7360948 7.7600217 50-59
CCTTG 5876405 1.7346774 23.283993 20-24
TGCAG 5576585 1.7276651 13.61171 150-159
GTCAC 4958520 1.7222455 8.584843 130-139
AAAAA 1937365 1.715007 8.254124 8
AGTCT 4605245 1.6931715 13.440591 210-219
GGTCA 5446455 1.6873502 7.5409064 130-139
ATAGT 3655700 1.6740175 7.7344165 220-229
CACCA 3643450 1.6693351 13.859693 230-239
TGACT 4520655 1.6620709 11.860982 190-199
AACTG 3815475 1.6505659 10.724445 160-169
TGGTT 5913515 1.6481856 18.122152 10-14
TTCAG 4468455 1.6428791 9.2386675 150-159
CAAAA 2291200 1.6284566 21.580639 70-79
GAGAA 3565760 1.6189024 10.429863 160-169
TCCCA 4143920 1.6136377 11.060455 240-247
GTCCC 5783860 1.6129448 20.552872 50-59
CTGTG 6094300 1.6046454 5.5915565 210-219
GTCTG 6084055 1.6019478 9.273179 210-219
GCTCC 5717415 1.5944153 6.484412 150-159
TGTGC 6041565 1.59076 8.414775 220-229
CAAAG 3118295 1.5872213 29.075586 70-79
TTGGT 5690710 1.5860865 24.533474 25-29
AATAG 2938640 1.5833315 9.72847 240-247
TCTGG 6009275 1.582258 12.067827 180-189
TGCTC 5355065 1.580781 5.4379973 150-159
ACTGA 3631425 1.5709462 8.743879 180-189
GAACC 3832455 1.566232 12.384988 70-79
TAGTT 4017185 1.5634185 10.185456 220-229
CTGGT 5919070 1.5585068 6.958476 160-169
GCTCA 4463140 1.5501848 11.14561 140-149
TTGTA 3971705 1.5457183 9.103246 210-219
GGCCG 7365990 1.543918 12.950668 120-129
ACCAT 3182040 1.5432687 11.837825 45-49
GTGCT 5855675 1.5418147 8.15238 220-229
ATAGA 2842735 1.5316582 10.109792 240-247
GGACG 5787865 1.5109593 7.141088 170-179
CAGAC 3654540 1.4935224 16.256401 60-69
AGATC 3426405 1.4822551 7.273485 130-139
CCACC 4009505 1.47496 9.919487 230-239
CCTCG 5287910 1.4746393 9.58983 130-139
CATGT 4006090 1.4728853 7.9388075 70-79
GCTCT 4988720 1.4726384 10.01958 130-139
CCCAC 3974460 1.4620682 10.511092 240-247
GCTGA 4714955 1.4607263 21.445652 30-34
AAATA 1896185 1.4265897 5.3071456 9
CGACT 4065130 1.4119438 8.678208 190-199
TATAC 2742725 1.4080672 6.7194953 110-119
AATTG 3051385 1.3972896 9.284153 170-179
TGATA 3034440 1.3895302 7.6908846 180-189
TCGAC 3983510 1.3835946 9.310432 190-199
CCTCA 3551845 1.3830844 7.5451674 130-139
TGGAC 4458470 1.3812654 10.344576 170-179
CACTG 3956100 1.3740743 7.343147 230-239
CTGCT 4630545 1.3669075 5.732607 90-99
CTCTT 3897520 1.3653759 8.107205 200-209
GTGGA 4870795 1.3459826 21.72463 25-29
GGGAG 5749605 1.3388153 6.4430785 150-159
GCGTA 4316905 1.3374076 10.422214 210-219
GATCT 3634520 1.3362732 5.7352247 130-139
CCATG 3846175 1.335894 8.069519 70-79
ACCGT 3830825 1.3305626 19.22136 45-49
CCATC 3413765 1.3293163 11.463269 240-247
GTAGG 4781730 1.3213706 5.29467 160-169
ACTTC 3203945 1.3206416 7.0337605 220-229
CTGAT 3583215 1.3174102 7.3677955 180-189
GAGGG 5633475 1.3117741 8.0956335 200-209
GAATT 2841240 1.3010602 10.307298 170-179
ACGGA 3565400 1.2996775 9.450617 200-209
CTCGA 3734490 1.2971025 8.311555 190-199
ATCGG 4161455 1.2892481 8.153812 190-199
CTTGA 3505070 1.2886794 8.247576 200-209
TATGG 3920860 1.2858131 6.0874796 180-189
AGCCG 4386890 1.2839342 9.140156 120-129
TCCAC 3292450 1.2820764 9.824201 240-247
GTCCA 3667025 1.2736697 16.859514 70-79
GGAAT 3273465 1.2631073 8.633518 170-179
GTGTT 4524560 1.261063 7.5155168 160-169
ACGTG 4033845 1.2497135 9.259496 170-179
AAAGT 2317560 1.2486953 21.396107 70-79
AAGTA 2312885 1.2461762 15.7562475 70-79
GGCTC 4996910 1.2429439 8.281716 130-139
GGAGT 4490485 1.2408887 7.0600038 210-219
ATAAC 2039830 1.2321715 7.608453 80-89
ACTAT 2399685 1.2319565 6.364849 230-239
GGAGG 5257725 1.2242794 16.177916 30-34
TTGGC 4645280 1.2231145 8.312443 60-69
GTGGT 5197440 1.2206553 10.912863 50-59
TCACG 3491935 1.2128557 9.612144 200-209
TGGTC 4602750 1.2119162 12.384248 50-59
ACTCG 3451820 1.1989225 8.500723 190-199
GCATA 2752505 1.1907274 8.37542 210-219
ATAAA 1571835 1.1825659 5.664916 7
ACTGG 3804775 1.1787461 7.0923376 160-169
TGCCC 4217790 1.1762149 6.8579335 60-69
TTGAG 3551315 1.1646239 8.0021 200-209
ATACC 2399615 1.1637977 7.778466 70-79
AGCTT 3164295 1.1633896 6.0787697 150-159
TTCCA 2821000 1.1627945 7.9171524 230-239
AGAGC 3178700 1.1587157 6.950333 140-149
TATCA 2228140 1.1438882 6.988927 70-79
CCATA 2343835 1.1367447 10.718179 70-79
AGTTC 3088925 1.1356789 5.587208 160-169
ACTGT 3076975 1.1312854 5.668976 230-239
GAAGA 2479980 1.1259439 18.38576 35-39
ATATC 2185630 1.1220644 7.5053425 70-79
TCTGT 3589435 1.1216006 6.8473983 210-219
AGGTT 3409565 1.1181382 23.247948 10-14
CAATA 1843275 1.1134412 6.629636 100-109
CTGAA 2528260 1.0937195 14.674095 35-39
AAAAG 1724710 1.0933963 14.401203 70-79
GTCTA 2971695 1.0925778 7.317608 180-189
TCTAC 2641620 1.0888555 7.2603207 180-189
CGGCT 4351255 1.082342 7.753227 130-139
ATCCA 2224005 1.078628 16.052652 240-247
CTCGG 4324300 1.0756375 8.27238 130-139
GCTTC 3600570 1.0628653 5.424479 150-159
ATGGG 3843270 1.062039 30.99658 15-19
GAGCT 3408165 1.0558734 6.211561 140-149
TACTG 2862710 1.0525084 6.412674 180-189
TATGT 2694055 1.0484792 7.6593657 220-229
AGAGA 2301385 1.0448594 13.251705 35-39
AACTT 2027600 1.0409346 6.2624435 200-209
CTGCC 3709845 1.0345644 5.993307 200-209
GTCCG 4156210 1.0338262 5.012957 130-139
CATGG 3294315 1.020602 5.4732275 190-199
CTTCT 2911140 1.0198281 5.7850566 210-219
GCGGT 4579385 1.0160276 5.7017207 130-139
CTGCG 4070845 1.0125923 6.8822346 210-219
TAGAT 2193410 1.004406 7.819239 240-247
CTACC 2578120 1.0039171 8.639298 230-239
ATACG 2289230 0.99031574 5.2123833 120-129
GGGGT 4977480 0.9850453 7.619995 10-14
CGAAC 2399365 0.98056257 13.023919 70-79
AAGAG 2144550 0.97365415 13.707035 35-39
AAAAT 1288500 0.9693996 5.4760494 8
TGCGT 3675380 0.96773726 8.333558 210-219
TATAA 1511615 0.9665483 6.544783 240-247
AGCTG 3107770 0.962809 5.0644727 90-99
TAGGC 3102695 0.9612368 5.9775243 160-169
TCCCC 3072455 0.960592 5.050346 80-89
CAGCG 3273895 0.9581881 6.209517 160-169
ACAAT 1571320 0.9491652 7.630283 100-109
CCATT 2291800 0.94466233 5.9179993 50-59
ACCCA 2060750 0.9441827 13.387955 240-247
CGTGG 4252505 0.9435028 7.555259 50-59
GAGTT 2837455 0.9305195 5.321533 160-169
ACGTC 2674010 0.92876536 11.571922 70-79
AGTTT 2375265 0.9244118 5.1445074 220-229
CGGAG 3540440 0.92425454 6.63021 210-219
TGTAA 2005965 0.9185711 6.9929943 230-239
TGCAT 2451700 0.90139574 6.414237 210-219
TCGAA 2076180 0.89815074 16.523806 70-79
CCCAA 1958560 0.8973619 9.956897 240-247
TGAAG 2323020 0.8963662 9.652509 35-39
TTCTG 2866170 0.8956001 5.7486587 210-219
ACTAA 1476635 0.89197016 12.7502 240-247
TGAAC 2030180 0.87825125 6.5405064 190-199
GAGGC 3304455 0.86264914 13.713585 30-34
CATCA 1768660 0.8577886 5.79799 230-239
GGTGG 4334040 0.8577082 21.463593 25-29
ATCAA 1417720 0.8563822 13.123157 70-79
CCAAT 1742225 0.8449678 10.867806 240-247
CCAGC 2574750 0.8448369 5.6408954 80-89
AGCGT 2699820 0.8364232 6.4534225 160-169
CGGTC 3347015 0.8325449 5.455398 130-139
GTATG 2512525 0.82396144 5.3142767 220-229
GGGCC 3881175 0.8134978 16.368225 15-19
CTTCC 2446105 0.8095315 5.5508404 230-239
GACCG 2762450 0.8085008 17.687778 45-49
CGTCC 2881370 0.8035276 9.495348 70-79
TAACC 1646065 0.79833084 6.0994844 80-89
AACCG 1889840 0.7723321 5.7145176 190-199
TGGGC 3440130 0.76326126 17.764767 15-19
TGAGA 1969655 0.7600159 5.922544 240-247
GGTCC 3054695 0.7598325 12.514782 50-59
AAAGG 1654870 0.75133294 8.698191 10-14
AAAGA 1168600 0.740845 5.9667253 8
AGCCA 1807455 0.7386632 5.7415133 80-89
GTCGA 2336825 0.7239648 11.763943 70-79
CATAC 1484135 0.7197958 9.262429 70-79
AATCC 1483175 0.71933025 9.729992 240-247
ATGAT 1569700 0.7187968 7.213864 240-247
CTAAT 1390345 0.7137789 9.778822 240-247
GTAGC 2299485 0.7123966 5.5250416 80-89
AAGGT 1827255 0.70506907 9.775301 10-14
AAAGC 1345950 0.68509245 9.82481 70-79
GGAAG 2105510 0.68459374 6.2917886 30-34
CATTG 1839075 0.6761572 5.3829675 50-59
TGTTA 1736930 0.6759829 5.024926 230-239
AGACC 1629895 0.6660988 5.196414 240-247
AGGAT 1668095 0.64365524 5.850061 10-14
ATCCC 1650255 0.64260745 5.1246657 240-247
TAGGT 1950985 0.63980913 5.5932164 10-14
CCCAT 1637120 0.6374927 5.1189065 240-247
ATAGG 1611970 0.6219987 5.2863383 10-14
CAATC 1280365 0.6209687 9.294967 240-247
GAGGT 2245115 0.62040913 6.7908897 10-14
TATGA 1354745 0.6203646 5.7868524 240-247
CCGTA 1783340 0.6194084 5.2155375 80-89
CGATG 1988810 0.6161473 13.568802 15-19
AGAAG 1338685 0.60778075 5.657329 9
AATGG 1526290 0.58893806 6.683431 10-14
ACGAT 1345135 0.5819023 6.125177 10-14
GGGTC 2556100 0.5671216 5.6402316 70-79
CATCC 1449360 0.56437916 7.675251 240-247
AAATG 1032155 0.5561224 5.324782 9
TAAAA 735060 0.5530204 6.4810443 8
GAAAA 865970 0.54898983 5.772877 8
GAAGG 1635675 0.5318298 5.4343877 10-14
AAGCA 1027735 0.52312005 6.5936646 70-79
AAGAA 812735 0.515241 5.317322 9
AAGGG 1563540 0.50837547 6.2634835 10-14
TGATC 1343890 0.4940966 5.788391 240-247
GGTCG 2185270 0.4848456 5.711338 70-79
GATCC 1366280 0.47455075 5.867178 240-247
ATAAG 863385 0.46518958 5.5644684 9
CGGTT 1686130 0.44396248 8.016888 10-14
TAAGG 1144175 0.4414942 5.9924726 10-14
GAAGC 1141355 0.41605246 5.052322 30-34
AAGCT 940850 0.4070095 8.330644 30-34
TAAAG 745450 0.4016465 5.5812654 9