FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_1_SL248338.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_1_SL248338.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6180083
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7156505 9.13313 63.077003 110-119
ATACA 6654055 7.9924545 38.71509 110-119
CACAG 9675485 7.8014503 43.18046 110-119
TCCCT 11152240 7.197572 107.164314 30-34
CAGTA 8010855 6.8628793 40.07611 110-119
AGTAA 6271255 6.7434196 49.228184 110-119
GGAGA 9962240 6.4375925 96.92953 35-39
GTAAT 6778185 6.1697235 40.769535 110-119
GGTGA 11002185 6.0182743 103.739685 45-49
CCCTG 10854760 5.902707 70.06661 30-34
AATAC 4784640 5.7470245 40.965275 110-119
GTAGT 8672930 5.6305633 33.3906 80-89
AGACG 7697500 5.5562816 103.81417 40-44
ACAGT 6401720 5.4843373 36.49903 110-119
GTGAC 8506245 5.197554 86.57291 45-49
TAGTA 5566540 5.066846 43.509968 80-89
CCCCA 6804695 4.8829365 102.53306 60-69
GAGAC 6759560 4.8792486 143.86838 40-44
CTCAG 7083435 4.8347406 35.622486 140-149
ACTCC 6201055 4.7278414 120.44354 30-34
AGTAG 5925020 4.544114 34.96616 80-89
GCACA 5588510 4.506078 35.010674 110-119
ACCAG 5587735 4.5054526 92.189316 45-49
CGTGT 8704580 4.502312 23.526701 120-129
GGCCC 9707195 4.447663 58.324623 60-69
GTGTC 8580440 4.438102 23.683008 120-129
TGACC 6467085 4.4140553 148.04567 45-49
CACTC 5738070 4.37485 99.28919 25-29
CTCCC 6993370 4.2480106 69.016365 30-34
ATGCA 4814270 4.124373 97.42814 25-29
CCCAG 6354750 4.08228 88.16278 60-69
GACCA 4943560 3.9860473 105.78811 45-49
TACAC 4108610 3.9317915 28.44457 110-119
TGAGG 7177055 3.9259007 70.448326 35-39
AGGAG 5911610 3.8200784 97.27269 35-39
AGATA 3550825 3.8181677 20.330786 150-159
CCCTT 5910815 3.8147953 37.210167 20-24
ATGGT 5867085 3.8089774 26.602589 190-199
ACGGT 6182685 3.7777944 98.48308 40-44
GACGG 7289425 3.7528527 109.73564 40-44
CCACT 4881320 3.7216418 29.76603 240-247
CTGAG 6073310 3.710963 106.352394 35-39
CAGGG 7143695 3.6778257 34.589516 50-59
TGTCC 6293345 3.636113 20.602427 120-129
GAGGA 5610560 3.62554 127.5505 35-39
TGCAC 5210995 3.5567214 116.24034 25-29
TGGTG 7570920 3.505648 18.363325 190-199
TCTTG 5686140 3.4905849 18.895134 170-179
GCCCC 6816975 3.4889748 73.08469 60-69
CTTGG 6723355 3.4775534 21.290691 20-24
CCGTG 7139640 3.4756756 23.553415 120-129
CCTGA 5058955 3.4529483 85.844215 30-34
GGGTT 7404075 3.4283924 40.789238 50-59
CTCTC 5282330 3.4091763 14.069043 130-139
AGGCT 5537380 3.3834944 26.494215 30-34
GGATG 6182285 3.3817546 70.78644 20-24
CAGAT 3920355 3.3585584 18.476143 150-159
TCAGG 5455955 3.3337414 21.266392 140-149
GCACT 4861375 3.3180916 90.12041 25-29
CTTCA 4092795 3.3154473 17.368015 200-209
TCGCA 4839410 3.3030996 24.566418 100-109
CCAGG 5628050 3.236639 39.101486 50-59
TGTCT 5262190 3.2303321 20.551817 180-189
CGCAC 4981600 3.2001715 24.93148 100-109
TCTCA 3927615 3.1816401 22.183193 130-139
ACAGA 3101045 3.1384046 15.1697035 140-149
CGGAT 5058025 3.0905955 80.258705 20-24
GGTTG 6643065 3.0760133 73.428635 15-19
CCTGG 6290050 3.0620835 28.323349 50-59
GATGC 4859390 2.9692235 96.35572 25-29
GACTC 4340790 2.9627707 20.444916 190-199
ACCAC 3262250 2.9382446 20.591137 230-239
GGTTC 5673755 2.9346638 45.98703 50-59
TTCCC 4532995 2.925561 47.8261 50-59
GCCCT 5325055 2.8957102 19.865694 20-24
TTCTT 3968225 2.8911452 21.801205 170-179
TGGCC 5915180 2.8795912 42.889137 60-69
AGGGT 5228230 2.8598795 48.985935 50-59
TCTCT 4170555 2.859846 15.10907 180-189
GTTGG 6144745 2.8452706 52.986477 15-19
TCAAA 2343870 2.8153172 57.51648 70-79
GCCGT 5750995 2.799664 22.25935 120-129
TCACA 2917700 2.7921336 12.383619 140-149
CGGTG 6389330 2.784519 69.030815 40-44
CGGCC 6069375 2.7808788 12.986267 120-129
GATGG 5075270 2.776209 20.673206 15-19
TCCAT 3304675 2.6770158 21.233728 70-79
AGGGA 4086590 2.6407516 9.422913 150-159
AGATG 3439820 2.6381235 17.395157 180-189
CCAGT 3845100 2.6244414 34.9053 60-69
AGTCA 3062665 2.6237774 30.472801 70-79
GTTCC 4521345 2.6123025 51.933613 50-59
TGTTC 4177095 2.5642188 13.258956 140-149
TGGAG 4676900 2.5582979 18.049997 25-29
ACAGC 3163130 2.5504668 11.46377 120-129
GCGGA 4932805 2.539582 87.52046 20-24
TCCTC 3915790 2.5272214 16.963005 130-139
GGCTG 5745345 2.5038655 13.636695 30-34
ACTCT 3085105 2.4991488 14.405025 200-209
GTTCT 4039610 2.4798198 18.387218 160-169
GGCGG 6638755 2.437747 41.891575 20-24
CAGCT 3567205 2.4347665 12.766644 150-159
GAGAT 3167740 2.4294553 16.945137 180-189
TACCA 2504190 2.3964195 17.284561 230-239
TTGGG 5147795 2.3836415 79.78788 15-19
TGTAG 3668660 2.381735 8.562925 210-219
CACGG 4136005 2.378578 10.03348 120-129
CTACT 2897435 2.3471231 8.668274 230-239
CATTT 2722325 2.343079 18.23057 150-159
CTGGA 3827105 2.3384686 14.875893 180-189
AATAA 1549200 2.3356059 9.750903 110-119
ACTAC 2424405 2.3200681 14.298258 230-239
TCATT 2688215 2.313721 17.00208 150-159
TTCAT 2684850 2.3108249 16.514717 140-149
GATAC 2661265 2.279899 15.290224 150-159
CAGAG 3145760 2.2707024 9.812306 140-149
CTGGC 4615075 2.2466824 33.27349 60-69
GTGAA 2923515 2.2421503 18.38527 190-199
TCAGC 3282160 2.2402115 14.709186 140-149
CACTA 2312490 2.2129695 16.756376 240-247
ATTTG 2842665 2.190305 16.08869 150-159
TTGTC 3564250 2.1880078 13.384858 170-179
TTCAC 2681205 2.1719618 18.904121 200-209
CTCGC 3989885 2.1696584 16.642214 100-109
ATGTA 2360270 2.1483946 9.471068 70-79
AGCTC 3129400 2.1359463 12.86782 140-149
TACAG 2490265 2.1334038 14.523751 160-169
TTGGA 3262640 2.1181424 16.358147 170-179
ACACA 1872990 2.1174073 14.269508 110-119
GAACT 2461360 2.108641 11.521237 160-169
GATAT 2306405 2.0993648 9.051291 180-189
TGGGG 5361135 2.0916233 52.50207 15-19
TCTCG 3613350 2.0876892 16.013681 100-109
GTTCA 2837810 2.0579622 15.201892 140-149
CTCTG 3555855 2.0544703 14.673293 180-189
TAGTC 2826075 2.049452 25.42977 70-79
GCTGT 3959710 2.0481 10.389708 140-149
TCTGC 3543515 2.0473406 12.329992 210-219
GTCCT 3535780 2.0428712 14.387971 130-139
GAATC 2383670 2.042084 14.617929 190-199
ATATA 1598855 2.0404584 10.707859 110-119
ACGGC 3542500 2.0372586 14.901604 120-129
GTCTC 3520335 2.0339477 12.028441 180-189
ATATG 2231355 2.0310519 9.345223 180-189
CAGGC 3525795 2.0276518 15.359547 140-149
TTTGC 3287925 2.0183785 12.387501 150-159
AACCA 1780500 2.0128477 15.253305 70-79
ACACG 2492895 2.0100489 14.1159115 120-129
GACGT 3288190 2.0091765 10.205804 170-179
TACTA 1975965 2.0090911 11.568246 230-239
TGAAT 2207150 2.0090194 14.1746025 190-199
TTGCA 2762315 2.0032136 14.58118 150-159
AATCG 2335950 2.0012023 13.573385 190-199
ATCTC 2465295 1.9970597 7.791045 100-109
AATAT 1559445 1.9901633 13.421998 110-119
CCAGA 2455530 1.9799211 31.000635 60-69
GTCAA 2272805 1.9471061 38.71057 70-79
TGGAA 2534280 1.9436318 9.428374 170-179
CAGCC 3009615 1.9333717 10.377187 120-129
TCGGC 3927780 1.9120977 8.1012 130-139
CCTTC 2955880 1.9077028 14.214923 200-209
AGAAC 1874820 1.8974067 12.267407 160-169
GAGTC 3079885 1.8818961 12.713925 210-219
CATAG 2191835 1.8777395 8.593326 210-219
GGGGC 5078120 1.8646824 47.7352 15-19
AGTAT 2048370 1.8644929 13.03873 220-229
CTGTT 2983240 1.8313395 13.028587 140-149
GTTGT 3308585 1.8182555 7.442837 210-219
GGGCG 4947630 1.8167666 58.23272 20-24
CGTAG 2942490 1.7979441 9.959571 210-219
GCAGA 2475330 1.7867659 14.844615 150-159
CCTTG 3063255 1.7698603 24.188606 20-24
ATTGT 2294125 1.7676488 8.491419 50-59
GAGAA 1933780 1.7520232 11.374009 160-169
GGTCA 2865495 1.7508978 7.9594135 130-139
AACTG 2032610 1.7413318 11.322757 160-169
TGACT 2388235 1.731933 12.29532 190-199
GTCAC 2520120 1.720087 9.017174 140-149
AGTCT 2358700 1.7105144 13.5672865 210-219
TGCAG 2795955 1.7084069 13.358951 150-159
GTCCC 3123690 1.6986305 23.161734 50-59
TGGTT 3076655 1.6907967 18.3084 10-14
CAAAA 1187065 1.6843864 22.656599 70-79
ACTGA 1961045 1.6800222 9.416196 180-189
ATAGT 1842550 1.6771488 7.647056 220-229
TCCCA 2172675 1.6565026 11.640097 240-247
CTCCA 2153625 1.6419784 7.513066 150-159
GAACC 2024850 1.6326591 12.593313 70-79
CACCA 1811450 1.6315374 13.216472 230-239
TTGGT 2967225 1.6306589 25.622427 25-29
GCTCA 2381630 1.6255621 11.47166 140-149
TTGTA 2101785 1.6194488 9.630748 210-219
TTCAG 2222360 1.6116419 9.770942 150-159
AATAG 1496415 1.6090806 10.500565 240-247
CTGGT 3099195 1.6030116 7.235141 160-169
GGACG 3112720 1.6025379 7.516765 170-179
TAGTT 2073760 1.5978552 10.459768 220-229
TCAGA 1862960 1.5959932 10.0764475 130-139
GCTCC 2928635 1.5925616 6.12034 150-159
CAGAC 1973055 1.5908962 19.197754 60-69
GTGCT 3066500 1.5861003 8.772731 220-229
GGCCG 3845740 1.5774294 13.07263 120-129
TGTGC 3037260 1.5709766 8.440478 220-229
TCTGG 3035395 1.570012 11.909414 180-189
GTCTG 3034765 1.5696859 9.294744 210-219
ACCAT 1636460 1.566033 13.012598 45-49
TGCTC 2699945 1.5599501 5.1552105 150-159
CTGTG 2986335 1.5446365 5.37983 210-219
ATAGA 1436050 1.5441706 10.959317 240-247
GCTCT 2634775 1.5222969 10.353797 130-139
CAAAG 1495750 1.5137699 27.889902 70-79
CCTCG 2777005 1.5101069 9.72277 130-139
TGATA 1654540 1.506016 8.498517 180-189
CCCAC 2087310 1.4978192 11.027161 240-247
ACCGT 2175720 1.4850198 22.825678 45-49
CATGT 2039610 1.4791124 9.407545 70-79
CCACC 2053625 1.4736475 9.765479 230-239
CGACT 2158185 1.4730515 9.014893 190-199
GCTGA 2367945 1.4468808 22.570831 30-34
TCGAC 2116645 1.4446987 9.701947 190-199
AATTG 1569130 1.4282732 9.586214 170-179
CTCTT 2078395 1.4252036 8.794299 200-209
TATAC 1394400 1.4177765 7.0202127 110-119
TGGAC 2319860 1.4174995 10.493701 170-179
TATGG 2158120 1.4010757 6.7401066 180-189
GGGAG 3032735 1.3977665 6.759131 150-159
AAATA 925490 1.3952879 5.238295 9
CACTG 2020420 1.3790212 7.706279 230-239
CTCGA 2014070 1.374687 8.795888 190-199
GAGGG 2979270 1.3731247 8.526847 200-209
CTGAT 1883485 1.3658917 7.8807206 180-189
GTCCA 1988710 1.3573775 19.675795 70-79
CCATG 1970015 1.3446176 9.457423 70-79
GCGTA 2194825 1.3410997 10.472416 210-219
GTGGA 2450910 1.3406653 22.870228 25-29
CCTCA 1752980 1.3365165 7.129057 130-139
GAATT 1467185 1.3354794 10.613059 170-179
ACGGA 1845205 1.3319232 9.542539 200-209
ACGTG 2179805 1.331922 9.775996 170-179
GTAGG 2420360 1.3239543 5.018465 160-169
CTTGA 1825115 1.3235621 8.98401 200-209
AGCCG 2278460 1.3103211 9.191232 120-129
AGATC 1521410 1.3033881 7.0009546 130-139
CCATC 1696465 1.2934278 11.169915 240-247
TTGGC 2495905 1.290969 9.283219 60-69
ACTCG 1886150 1.2873763 9.066585 190-199
ACTTC 1585795 1.2846038 6.7991266 220-229
GTGTT 2336405 1.2839873 7.6214385 160-169
ACTAT 1259745 1.280864 6.9416823 230-239
CTGCT 2214755 1.2796215 5.7128983 90-99
TGGTC 2472975 1.2791086 14.577626 50-59
GGCTC 2622705 1.2767692 8.631449 130-139
GTGGT 2749125 1.2729582 12.907769 50-59
GGAGG 2741560 1.2635659 17.122702 30-34
GATCT 1740515 1.2622106 5.588853 130-139
AGCTT 1737445 1.2599843 6.878548 150-159
ACTGG 2061195 1.2594479 7.501052 160-169
AAAAA 704770 1.2551998 8.607896 8
TCCAC 1644140 1.253534 10.120874 240-247
ATAAC 1040805 1.250153 7.443038 80-89
AAGTA 1157515 1.2446647 15.883495 70-79
ATCGG 2033280 1.2423911 8.0997715 190-199
TTGAG 1904740 1.2365786 8.692043 200-209
GGAGT 2231410 1.2205974 7.1715536 210-219
CCATA 1274945 1.2200763 12.403525 70-79
GGAAT 1589495 1.2190418 8.805479 170-179
ATACC 1269995 1.2153394 9.092754 70-79
TGCCC 2221345 1.2079445 8.072331 60-69
TCTAC 1487520 1.2049943 8.052456 180-189
AGAGC 1667270 1.2034845 7.5833697 140-149
GTCTA 1631765 1.1833458 8.064762 180-189
AAAGT 1093210 1.1755183 20.431246 70-79
ATAAA 771895 1.1637248 5.7127957 7
TCACG 1702945 1.1623311 9.609311 200-209
GCATA 1339170 1.1472635 7.933525 210-219
GCTTC 1977795 1.1427128 6.034428 150-159
GAGCT 1865925 1.1401325 6.7960916 140-149
TACTG 1571460 1.139613 7.0759306 180-189
AAAAG 888735 1.1289414 15.61128 70-79
AGTTC 1556250 1.1285828 5.6098776 160-169
TATCA 1108335 1.1269157 7.5364914 70-79
AGGTT 1723115 1.1186656 23.772356 10-14
TTCCA 1379610 1.1175797 7.779297 230-239
ACTGT 1537030 1.1146445 5.830126 230-239
CTCGG 2261975 1.1011608 8.412302 130-139
CGGCT 2254520 1.0975317 8.033291 130-139
ATGGG 1976410 1.0811105 32.55826 15-19
CAATA 895885 1.0760837 6.498225 100-109
ATATC 1056530 1.0742422 7.9817023 70-79
ATCCA 1118825 1.0706751 16.321848 240-247
TCTGT 1741155 1.0688533 6.413597 210-219
AGAGA 1177520 1.0668445 14.315897 35-39
GAAGA 1173535 1.063234 18.993849 35-39
CTACC 1387275 1.0576936 9.095598 230-239
TATGT 1368565 1.0544946 8.024411 220-229
CTGCG 2165330 1.0541127 7.164084 210-219
GTCCG 2142160 1.0428331 5.2210546 130-139
GCGGT 2361100 1.0289855 5.955506 130-139
AACTT 1008415 1.0253206 6.0765066 200-209
ATACG 1192815 1.0218816 5.422822 120-129
CTGAA 1191360 1.0206351 15.566632 35-39
CGAAC 1259605 1.0156336 13.224433 70-79
TAGAT 1100240 1.0014741 8.360129 240-247
CTGCC 1834325 0.99748707 5.653114 200-209
TATAA 778905 0.99403834 7.0387735 240-247
CATGG 1623170 0.9918024 5.3951745 190-199
TCCCC 1628915 0.9894584 5.409481 80-89
CGTGG 2267060 0.988002 8.844873 50-59
TGCGT 1905255 0.9854641 8.405145 210-219
ACGTC 1441350 0.98378164 13.520417 70-79
AAAAT 651560 0.9823052 5.3373137 8
GGGGT 2508610 0.9787233 7.5064163 10-14
CAGCG 1675425 0.9635213 6.4958515 160-169
AGCTG 1568715 0.9585289 5.176534 90-99
CTTCT 1373145 0.9415973 5.496537 210-219
ACTAA 782030 0.93932784 13.578751 240-247
CCCAA 1041415 0.937982 10.649373 240-247
GAGTT 1444320 0.93766874 5.3799815 160-169
CGGAG 1814650 0.9342457 6.7077856 210-219
ACAAT 776715 0.9329438 7.518874 100-109
CCATT 1151650 0.93291634 6.3808784 50-59
AAGAG 1016135 0.92062813 14.28992 35-39
ACCCA 1020600 0.9192344 13.291561 240-247
TGTAA 1009885 0.9192301 7.1271763 230-239
TAGGC 1497965 0.91529864 5.62372 160-169
TGAAG 1190660 0.91316056 10.315392 35-39
TCGAA 1061370 0.90927297 17.113878 70-79
CGGTC 1825855 0.8888516 5.7679687 130-139
ATCAA 734480 0.88221365 14.189736 70-79
CATCA 916540 0.8770957 5.6094465 230-239
TGCAT 1206990 0.8753016 6.128948 210-219
CGTCC 1602695 0.8715291 10.965665 70-79
TACAA 720755 0.86572796 5.008057 100-109
GGTGG 2217460 0.86513233 22.525719 25-29
GACCG 1489635 0.8566752 20.625593 45-49
GAGGC 1660710 0.8549919 14.58299 30-34
CCAAT 885185 0.84709007 11.269862 240-247
AGCGT 1374995 0.8401606 6.6798043 160-169
TGAAC 974145 0.8345475 6.6946874 190-199
GGTCC 1693765 0.82454824 14.506836 50-59
GGGCC 1998235 0.8196276 16.98037 15-19
TTCTG 1330380 0.81668836 5.3420243 210-219
CCAGC 1267735 0.81439084 5.6057477 80-89
AACCG 998485 0.80508953 5.8845053 190-199
GTATG 1236380 0.80267173 5.569554 220-229
TAACC 826025 0.79047614 5.9126887 80-89
CTTCC 1215520 0.7844875 5.589578 230-239
CATAC 814695 0.7796337 10.909384 70-79
TGGGC 1781645 0.7764544 18.49869 15-19
GGTGC 1758080 0.7661846 5.22065 220-229
CTAAT 731145 0.7434023 10.294439 240-247
AAAGG 812985 0.7365723 9.044702 10-14
AATCC 763070 0.73023045 10.087319 240-247
ATGAT 796665 0.72515035 7.087615 240-247
GTAGC 1181375 0.72185326 5.699989 80-89
AGCCA 893755 0.7206446 5.7645235 80-89
AAAGC 707220 0.71574026 10.598896 70-79
AAAGA 562685 0.714767 5.86701 8
CCTTT 1040440 0.71345377 5.612406 50-59
GTCGA 1162765 0.710482 11.748969 70-79
TGAGA 918375 0.7043353 5.996078 240-247
AAGGT 897375 0.6882296 10.121838 10-14
TGTTA 892765 0.6878854 5.2187123 230-239
TAGGT 1041200 0.6759587 5.5534687 10-14
CATTG 931975 0.6758625 5.837354 50-59
CCGTA 977600 0.6672529 5.7200513 80-89
TATGA 731755 0.6660672 6.2785115 240-247
TCCAG 957895 0.6538034 5.037189 240-247
CAATC 668740 0.63996005 9.855326 240-247
AGGAT 818410 0.62766844 6.260778 10-14
GAGGT 1141035 0.6241543 6.9165187 10-14
CGATG 1019285 0.6228118 14.347768 15-19
GGAAG 938670 0.606568 6.270147 30-34
ATAGG 775830 0.59501237 5.3501196 10-14
AGAAG 655610 0.59398896 5.496942 9
AGACC 720210 0.58071333 5.1841054 240-247
AATGG 734725 0.5634874 6.7162924 10-14
CATCC 738380 0.5629596 7.589731 240-247
AAGCA 541805 0.54833233 7.20834 70-79
TAAAA 360780 0.5439193 6.276521 8
GGGTC 1226820 0.53465754 5.566148 70-79
ACGAT 606030 0.5191844 6.5800695 10-14
AAATG 482450 0.51877385 5.3878727 9
AAGAA 404275 0.51354206 5.3334045 9
AAGGG 775865 0.50136346 6.306366 10-14
GAAGG 772300 0.49905974 5.5703664 10-14
TTGCC 851740 0.49211076 5.746031 60-69
GAAAA 378585 0.4809086 6.234368 8
GGTCG 1089900 0.47498673 5.6547613 70-79
TGATC 651725 0.4726269 5.26776 240-247
TACCG 681785 0.46534675 5.7893105 70-79
ATAAG 424400 0.45635322 5.6689897 9
GATCC 656555 0.44812623 5.2422805 240-247
TAAGG 558115 0.42803878 5.9555006 10-14
CGGTT 819985 0.42412484 8.127198 10-14
GAAGC 577740 0.4170297 5.0706825 30-34
AAGCT 477025 0.4086661 8.601 30-34
TAAAG 356085 0.38289475 5.6456714 9