FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_17_SL248346.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_17_SL248346.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6485244
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7304765 8.786595 61.666256 110-119
ATACA 6673945 7.5906043 37.04005 110-119
CACAG 9725320 7.5130005 42.187374 110-119
TCCCT 11523280 7.260185 106.38159 30-34
CAGTA 8298125 6.779695 39.464745 110-119
AGTAA 6386350 6.4728746 47.886143 110-119
GGAGA 10146235 6.2246633 92.97786 35-39
CCCTG 11500000 6.1051946 69.50701 30-34
GTAAT 6934200 5.9916615 39.79359 110-119
GGTGA 11302210 5.911269 101.37176 45-49
AATAC 4822560 5.484934 39.239857 110-119
AGACG 7747505 5.33362 100.05953 40-44
GTAGT 8503775 5.278362 28.019688 80-89
ACAGT 6439290 5.2609987 35.830196 110-119
GTGAC 8693840 5.1024446 83.765045 45-49
GAGAC 7096765 4.8856306 139.90688 40-44
CCCCA 6924940 4.839055 101.35202 60-69
ACTCC 6522655 4.820472 120.92147 30-34
CTCAG 7188815 4.7344894 35.552666 140-149
ACCAG 6104485 4.7158346 93.74635 45-49
TAGTA 5249860 4.536267 35.746456 80-89
GCACA 5826090 4.5007687 34.36204 110-119
GGCCC 9993495 4.4704275 59.056255 60-69
TGACC 6667885 4.3914104 143.35922 45-49
GTGTC 8764135 4.385126 23.810005 120-129
CACTC 5932400 4.384253 98.13749 25-29
CGTGT 8723675 4.3648825 23.337337 120-129
AGTAG 5834645 4.248106 28.769186 80-89
CTCCC 7092895 4.225464 69.059906 30-34
GACCA 5330885 4.118213 106.04853 45-49
ATGCA 5025870 4.1062126 94.93708 25-29
CCCAG 6493360 4.0435677 86.27648 60-69
TGAGG 7303795 3.8200233 68.07569 35-39
AGGAG 6143185 3.7688124 93.29385 35-39
TACAC 4086200 3.7462702 26.872978 110-119
ACGGT 6374720 3.7413454 95.13855 40-44
CAGGG 7519420 3.7186136 35.52648 50-59
GACGG 7468450 3.6934068 106.25511 40-44
CTGAG 6292735 3.6932275 104.573586 35-39
ATGGT 5920780 3.6750755 26.45318 190-199
TGTCC 6478290 3.63733 20.641464 120-129
CCACT 4916220 3.6332598 28.618122 240-247
CCCTT 5726785 3.6081324 35.19814 20-24
TGCAC 5474580 3.60551 114.48564 25-29
AGATA 3555500 3.6036713 19.756527 150-159
GAGGA 5779810 3.545884 122.20181 35-39
GCCCC 6959775 3.4936235 72.79342 60-69
GGGTT 7813065 3.4837358 42.22234 50-59
CCTGA 5246760 3.4554698 83.98038 30-34
TGGTG 7573130 3.3767521 18.376894 190-199
CCAGG 6078275 3.3730772 40.441875 50-59
GGATG 6442330 3.36946 69.01985 20-24
CCGTG 7074970 3.3471637 23.617405 120-129
AGGCT 5700015 3.3453584 24.501444 30-34
GCACT 5069515 3.3387377 88.6953 25-29
CTCTC 5266070 3.3178608 13.909551 130-139
CTTCA 4243580 3.3167922 17.648922 200-209
CTTGG 6611295 3.3079548 19.888378 20-24
TCTTG 5563655 3.3037195 18.426722 170-179
CAGAT 4027335 3.2903945 18.303875 150-159
TCAGG 5556420 3.2610817 21.30255 140-149
CCTGG 6817505 3.2253573 29.563162 50-59
TCGCA 4876695 3.211748 23.949295 100-109
TCTCA 4033190 3.152351 22.096321 130-139
TGTCT 5288555 3.1403642 20.243427 180-189
CGCAC 5013340 3.1219242 24.380825 100-109
CGGAT 5270705 3.0933948 78.62367 20-24
TTCCC 4884070 3.077184 49.967255 50-59
GGTTG 6815935 3.0391295 73.29575 15-19
ACAGA 3121375 2.9913673 14.372026 140-149
GATGC 5090600 2.9876907 95.85697 25-29
GGTTC 5971180 2.987674 47.82905 50-59
TGGCC 6281130 2.9715986 43.551785 60-69
AGGGT 5643920 2.9518766 49.81435 50-59
TCTCT 4303575 2.8676205 15.69137 180-189
TCAAA 2519800 2.8658917 58.097176 70-79
ACCAC 3303805 2.8640013 20.461065 230-239
GCCCT 5390235 2.8616025 18.961262 20-24
AGTCA 3484050 2.846522 32.13753 70-79
GTTGG 6359270 2.8355093 52.183567 15-19
GACTC 4288945 2.8246613 19.832829 190-199
CCAGT 4281940 2.8200479 37.000183 60-69
CGGTG 6659670 2.8077328 67.12687 40-44
GCCGT 5934260 2.8074946 22.35451 120-129
TTCTT 3898375 2.7472398 20.945297 170-179
GATGG 5236445 2.7387595 19.356 15-19
CGGCC 6110665 2.7335064 12.731186 120-129
GTTCC 4860770 2.72915 54.316658 50-59
TCACA 2852570 2.6152651 11.578477 140-149
AGATG 3591315 2.6147757 17.390768 180-189
TCCAT 3293615 2.574297 17.398207 70-79
GAGAT 3506630 2.5531175 17.04397 180-189
GCGGA 5153095 2.5483837 85.84948 20-24
TCCTC 4029280 2.5386276 17.57472 130-139
GGCTG 6009900 2.5337882 12.627477 30-34
TGTTC 4237385 2.5161753 13.31861 140-149
AGGGA 4052745 2.4863384 9.043384 150-159
TGGAG 4727955 2.4728098 16.517473 25-29
ACAGC 3180945 2.4573424 11.229261 120-129
GGCGG 6892915 2.4487026 41.424343 20-24
ACTCT 3073310 2.4021065 13.883522 200-209
TTGGG 5378335 2.398124 78.55922 15-19
CTGGC 5044150 2.386384 35.00501 60-69
AATAA 1677120 2.366313 9.938647 110-119
GTTCT 3984020 2.3657265 17.877134 160-169
CAGCT 3569285 2.3506997 12.411162 150-159
TACCA 2554675 2.342152 17.06466 230-239
CACGG 4208810 2.3356364 9.618489 120-129
CTGGA 3952805 2.3199146 14.9792795 180-189
CATTT 2789345 2.3057318 18.249134 150-159
TCATT 2781240 2.299032 17.073565 150-159
TTCAT 2733870 2.259875 16.538963 140-149
TGTAG 3638310 2.2583282 8.056619 210-219
TCAGC 3422320 2.2539098 14.416007 140-149
TTCAC 2836810 2.2172573 19.528616 200-209
GTGAA 3009955 2.1914973 18.436207 190-199
ACTAC 2386485 2.187954 12.836649 230-239
CAGAG 3160955 2.1760986 9.375083 140-149
TTGTC 3661535 2.1742334 13.473518 170-179
CTACT 2779370 2.172362 7.66782 230-239
GATAC 2645265 2.161222 14.831627 150-159
AAAAA 1304350 2.1587172 13.605094 120-129
ATTTG 2928185 2.157028 16.00771 150-159
TCTGC 3827855 2.1492047 12.699653 210-219
CACTA 2319095 2.1261702 15.903946 240-247
ATCTC 2720025 2.1259778 9.373479 100-109
GAACT 2593880 2.1192396 10.924392 160-169
TAGTC 3040050 2.1174703 26.49607 70-79
TGGGG 5622285 2.1123538 51.717148 15-19
TCTCG 3759935 2.1110702 17.0448 100-109
CTCGC 3975205 2.110382 16.26617 100-109
GCTGT 4165735 2.0843215 10.381902 140-149
GTCCT 3706860 2.0812705 14.844984 130-139
TTGGA 3352980 2.0812216 16.174547 170-179
TACAG 2535165 2.0712686 14.0273905 160-169
CTCTG 3675120 2.0634494 15.040278 180-189
GTCTC 3665230 2.0578966 12.27926 180-189
ATATA 1705245 2.0511677 10.832017 110-119
GTTCA 2943520 2.0502346 15.408164 140-149
GTCAA 2508215 2.0492501 40.64044 70-79
GAATC 2507810 2.0489192 14.958695 190-199
AGCTC 3096485 2.0393176 12.371728 140-149
CAGGC 3673170 2.0383887 15.838158 140-149
ATGTA 2349585 2.030215 7.5667844 70-79
ACACG 2623875 2.026995 13.366016 120-129
GATAT 2334695 2.017349 8.20103 180-189
ACACA 1872160 2.013335 13.684613 110-119
AATAT 1669260 2.007883 13.544096 110-119
AACCA 1854685 1.994542 15.279097 70-79
TGAAT 2303605 1.9904851 14.231998 190-199
TTGCA 2853805 1.987746 14.590337 150-159
AATCG 2423990 1.9804368 13.775855 190-199
ACGGC 3564715 1.9782027 14.509823 120-129
TTTGC 3303805 1.9618121 12.406999 150-159
TGGAA 2694440 1.9617761 9.637411 170-179
CCTTC 3056630 1.9258145 14.603093 200-209
GAGTC 3239530 1.9012913 12.778438 210-219
CAGCC 3048810 1.8985654 10.344928 120-129
GGGGC 5312805 1.8873698 47.125824 15-19
TACTA 1945235 1.8861326 10.165792 230-239
TCGGC 3984525 1.885076 8.004733 130-139
CGTAG 3206925 1.8821553 10.119714 210-219
ATATG 2163055 1.8690392 8.468893 180-189
CATAG 2274335 1.8581666 8.537353 210-219
GGGCG 5181180 1.8406101 58.43292 20-24
CCAGA 2365680 1.8275342 26.285135 60-69
GACGT 3075430 1.8049804 9.750579 170-179
CTGTT 3037360 1.8035959 13.167352 140-149
AGTAT 2058460 1.7786615 12.336057 220-229
CTCCA 2396310 1.7709575 8.121264 150-159
GTCAC 2656255 1.7493861 8.538709 130-139
AGAAC 1824270 1.7482878 11.4286785 160-169
ATTGT 2365650 1.7426405 7.7545657 170-179
GTTGT 3292200 1.7421277 7.1116953 210-219
GCAGA 2518960 1.7341295 14.792435 150-159
TGCAG 2943265 1.7274123 13.762686 150-159
AGTCT 2459925 1.7133988 13.701455 210-219
CCTTG 3027760 1.6999799 22.704737 20-24
GGTCA 2881705 1.6912825 7.6434274 130-139
CACCA 1943365 1.6846635 14.04135 230-239
TTCAG 2401225 1.6725128 9.41373 150-159
ATAGT 1933055 1.6703024 7.817213 220-229
TGACT 2394840 1.6680654 12.057723 190-199
TCAGA 2035585 1.663104 10.412044 130-139
GTCTG 3290275 1.6462859 9.406632 210-219
AACTG 2013025 1.6446722 10.844175 160-169
TGGTT 3105735 1.6434561 17.870289 10-14
CAAAA 1219460 1.6268777 21.146553 70-79
GAGAA 1886305 1.6109662 10.40784 160-169
GTCCC 3011570 1.5988016 19.998457 50-59
TCCCA 2163170 1.5986588 11.187373 240-247
CTGTG 3192640 1.5974342 5.5113125 210-219
GAACC 2061285 1.592383 12.634181 70-79
GCTCC 2988490 1.5865488 6.533513 150-159
TGCTC 2825350 1.5863339 5.55091 150-159
TGTGC 3167945 1.5850782 8.551531 220-229
CAAAG 1651355 1.5825748 29.065874 70-79
TCTGG 3155820 1.5790113 12.285175 180-189
TTGGT 2982825 1.5784162 24.142262 25-29
AATAG 1554930 1.5759968 9.733268 240-247
GGCCG 3917575 1.5617075 13.0694475 120-129
ACTGA 1909405 1.5600132 8.801593 180-189
AGATC 1906520 1.557656 7.1661468 130-139
CTGGT 3108155 1.5551622 7.0541387 160-169
GCTCA 2348800 1.546899 11.165034 140-149
TAGTT 2099860 1.5468478 10.3088 220-229
TTGTA 2095385 1.5435514 9.132487 210-219
GTGCT 3074505 1.5383255 8.294595 220-229
ATAGA 1505620 1.5260187 10.231621 240-247
ACCAT 1653865 1.5162804 11.507439 45-49
GGACG 3056105 1.5113496 7.2890058 170-179
CCTCG 2804525 1.4888844 9.871392 130-139
GCTCT 2649475 1.4875863 10.216751 130-139
CAGAC 1915000 1.4793751 15.51578 60-69
CCACC 2099030 1.4667737 10.092442 230-239
CATGT 2073600 1.4443138 7.62981 70-79
CCCAC 2060585 1.4399089 10.430001 240-247
GCTGA 2432520 1.4276544 20.872883 30-34
AAATA 1009375 1.4241657 5.330191 9
TATAC 1465985 1.4214438 6.8676147 110-119
AATTG 1638645 1.4159102 9.635079 170-179
CGACT 2148360 1.4148909 8.668445 190-199
CTGCT 2476435 1.3904303 5.8069797 90-99
TCGAC 2101695 1.3841578 9.39094 190-199
TGGAC 2346875 1.377389 10.43623 170-179
CCTCA 1858665 1.3736191 7.6779633 130-139
CTCTT 2060050 1.3726822 8.300745 200-209
TGATA 1587460 1.3716828 7.759912 180-189
GATCT 1968520 1.371123 5.6273108 130-139
GCGTA 2329910 1.3674322 10.59463 210-219
CACTG 2073845 1.365816 7.359327 230-239
GGGAG 3017865 1.3299865 6.461653 150-159
ACGGA 1927755 1.3271258 9.652451 200-209
GAATT 1531975 1.3237396 10.798525 170-179
GAGGG 3000820 1.3224746 8.116819 200-209
GTGGA 2524510 1.3203664 21.141811 25-29
ACTTC 1684430 1.3165543 7.016296 220-229
CTTGA 1889720 1.3162369 8.4330015 200-209
CCATC 1776120 1.3126153 11.420064 240-247
CCATG 1992930 1.3125261 7.703485 70-79
GGAAT 1799945 1.3105093 8.969354 170-179
GTAGG 2501710 1.3084418 5.2705164 160-169
ATCGG 2216325 1.3007687 8.015651 190-199
CTCGA 1966995 1.2954454 8.420602 190-199
CTGAT 1858325 1.2943695 7.441397 180-189
ACCGT 1965035 1.2941548 18.247807 45-49
TATGG 2065255 1.2819203 6.137787 180-189
GTGTT 2404630 1.2724537 7.7780323 160-169
AGCCG 2291955 1.2718972 9.15518 120-129
TCACG 1928230 1.2699151 10.004279 200-209
AAGTA 1237410 1.254175 15.943861 70-79
ACGTG 2135340 1.2532383 9.350983 170-179
GGCTC 2644520 1.2511207 8.506584 130-139
GTCCA 1894140 1.247464 16.289068 70-79
TCCAC 1686855 1.2466452 9.704559 240-247
GGAGT 2383460 1.2465945 7.287914 210-219
AAAGT 1227515 1.2441458 21.399446 70-79
ATAAC 1087105 1.2364178 7.627819 80-89
ACTAT 1271185 1.2325624 6.374026 230-239
GGAGG 2739590 1.2073494 15.934595 30-34
ACTCG 1829780 1.2050769 8.641992 190-199
AGAGA 1402710 1.1979604 12.83371 35-39
TGGTC 2388270 1.1949685 11.9349165 50-59
TTGGC 2373420 1.1875384 8.102354 60-69
GTGGT 2661990 1.1869439 10.505261 50-59
ATAAA 840995 1.1865921 5.360046 8
AGAGC 1723315 1.1863829 7.0135665 140-149
ACTGG 2015210 1.1827337 7.2387404 160-169
TGCCC 2203660 1.1698931 6.6307263 60-69
TTGAG 1879675 1.1667293 8.126244 200-209
GCATA 1420025 1.1601821 8.272415 210-219
AGCTT 1664590 1.1594282 6.1244683 150-159
ATATC 1189530 1.1533883 7.24303 70-79
TTCCA 1472630 1.1510111 7.9166527 230-239
GAAGA 1345845 1.1493957 18.601435 35-39
TATCA 1178695 1.1428826 6.782903 70-79
ATACC 1245565 1.1419467 7.407594 70-79
CCATA 1225555 1.1236013 10.286915 70-79
TCTGT 1880145 1.1164372 6.8462806 210-219
CAATA 976230 1.1103142 6.645829 100-109
ACTGT 1593765 1.1100968 5.6539736 230-239
AGTTC 1592110 1.108944 5.390867 160-169
CTGAA 1354775 1.1068718 14.381949 35-39
AGGTT 1775995 1.1023743 22.77172 10-14
CGGCT 2305420 1.0906926 7.9512362 130-139
TCTAC 1394360 1.0898349 7.2447624 180-189
GTCTA 1564170 1.0894831 7.3699484 180-189
CTCGG 2302000 1.0890746 8.489352 130-139
AAAAG 896460 1.0657834 14.028356 70-79
GCTTC 1888295 1.0602107 5.4915566 150-159
GAGCT 1791775 1.0515989 6.3203664 140-149
TACTG 1509255 1.0512335 6.4613347 180-189
CTTCT 1574115 1.0488871 5.842456 210-219
ATGGG 2001490 1.0468171 30.302843 15-19
AACTT 1074640 1.0419891 6.3131905 210-219
CTGCG 2196465 1.0391461 7.1110697 210-219
ATCCA 1133375 1.0390898 15.640496 240-247
CTGCC 1954780 1.037766 6.128365 200-209
TATGT 1396250 1.0285383 7.7415376 220-229
GTCCG 2160535 1.0221477 5.001322 130-139
GCGGT 2420825 1.0206256 5.8038354 130-139
CTACC 1373680 1.0151979 8.833278 230-239
CATGG 1720400 1.0097086 5.4397225 190-199
ATACG 1227680 1.0030333 5.319095 120-129
GGGGT 2654095 0.9971724 7.5714498 10-14
TAGAT 1143645 0.9881938 7.8540297 240-247
AAAAT 698815 0.98598474 5.4763117 8
AAGAG 1153580 0.9851951 13.851148 35-39
TGCGT 1965235 0.9833034 8.484259 210-219
CGAAC 1271380 0.982166 13.254683 70-79
CAGCG 1769305 0.9818579 6.479688 160-169
TATAA 812590 0.9774305 6.6515083 240-247
AGCTG 1626425 0.9545543 5.138016 90-99
ACAAT 838970 0.9542017 7.6986837 100-109
TCCCC 1595750 0.9506391 5.004178 80-89
TAGGC 1615230 0.94798404 6.0144877 160-169
CGGAG 1898750 0.9389975 6.847099 210-219
ACGTC 1423625 0.93758684 11.185541 70-79
CGTGG 2210630 0.93200696 7.30118 50-59
TGAAC 1138790 0.93040895 6.7082143 190-199
TTCTG 1553870 0.92269397 5.743511 210-219
CCATT 1178435 0.9210676 5.8245115 50-59
AGTTT 1243040 0.91567713 5.185095 220-229
TCGAA 1116040 0.9118217 16.762365 70-79
GAGTT 1468090 0.91125524 5.151494 160-169
ACCCA 1034750 0.8970037 12.868874 240-247
TGTAA 1038055 0.8969562 6.9291797 230-239
TGCAT 1278470 0.89048606 6.3259597 210-219
ACTAA 781795 0.8891738 12.79787 240-247
CCCAA 1023125 0.88692623 9.834898 240-247
TGAAG 1211925 0.88238215 9.345175 35-39
AGCGT 1473475 0.8647875 6.7056723 160-169
GTATG 1380690 0.85700536 5.4119024 220-229
CCAGC 1365980 0.8506277 5.727061 80-89
CATCA 927575 0.8504102 5.9278226 230-239
GAGGC 1716930 0.8490813 13.679745 30-34
CGGTC 1789070 0.84640783 5.540136 130-139
GGTGG 2242425 0.8425035 21.023722 25-29
ATCAA 730280 0.8305832 12.724896 70-79
CCAAT 899530 0.82469827 10.666985 240-247
GACCG 1475275 0.8186889 16.860535 45-49
TAACC 881840 0.8084799 6.1724052 80-89
GGGCC 2027580 0.8082773 15.973812 15-19
AACCG 1023875 0.7909635 5.957311 190-199
CTTCC 1254050 0.79010797 5.6580267 230-239
CGTCC 1484650 0.78818053 9.214693 70-79
TGAGA 1041550 0.758335 5.9047785 240-247
TGGGC 1793155 0.7559985 17.32247 15-19
GGTCC 1589520 0.75200087 12.120873 50-59
AAAGG 871855 0.74459267 8.568665 10-14
AGCCA 963240 0.7441218 5.9234166 80-89
AAAGA 619240 0.7362021 5.568554 8
GTCGA 1246520 0.7315869 11.895421 70-79
CTAAT 732115 0.70987105 9.87076 240-247
GGAAG 1149190 0.70502216 6.380695 30-34
ATGAT 815110 0.70431525 7.2189307 240-247
AGACC 910830 0.703634 5.1039987 240-247
GTAGC 1196135 0.7020158 5.4000278 80-89
CATAC 765335 0.701667 8.818188 70-79
AAGGT 956240 0.6962221 9.557025 10-14
AATCC 747575 0.6853844 9.388877 240-247
AAAGC 693710 0.6648164 9.572794 70-79
CATTG 949340 0.66123885 5.311946 50-59
AGGAT 870045 0.63346493 5.6684256 10-14
TAGGT 1010915 0.62748307 5.347833 10-14
ATAGG 856610 0.6236832 5.1854367 10-14
TATGA 713895 0.616858 5.9307995 240-247
GAGGT 1174040 0.6140451 6.714048 10-14
CAATC 661755 0.6067038 9.126356 240-247
CGATG 1029585 0.604267 13.257465 15-19
GAAAA 505125 0.60053307 6.1563616 8
GGGTC 1384030 0.5835104 5.6874065 70-79
AATGG 794700 0.5786076 6.5299125 10-14
AAATG 547390 0.5548062 5.1492305 9
CATCC 740045 0.54691935 7.579915 240-247
TAAAA 384000 0.5418003 5.6043735 8
AAGAA 444260 0.52817184 5.2961664 9
GAAGG 857580 0.52612096 5.397759 10-14
AAGGG 837105 0.51355964 6.1980834 10-14
AAGCA 524365 0.5025248 6.269288 70-79
GGTCG 1173695 0.49483263 5.772407 70-79
TGATC 692830 0.48257333 5.601594 240-247
GATCC 694610 0.457464 5.5248895 240-247
ATAAG 449045 0.45512882 5.3271246 9
ACGAT 556515 0.4546813 5.966506 10-14
CGGTT 895345 0.447985 7.958596 10-14
TAAGG 596590 0.43436706 5.701112 10-14
AAGCT 494810 0.40426734 8.1733675 30-34
TAAAG 394775 0.40012357 5.799087 9