FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11395985
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13007090 8.937575 62.426136 110-119
ATACA 11922460 7.725616 37.432076 110-119
CACAG 17489825 7.6476107 42.33586 110-119
TCCCT 20255995 7.1170974 103.50908 30-34
CAGTA 14536345 6.7401237 39.713196 110-119
AGTAA 11321860 6.5648856 48.398075 110-119
GGAGA 18098330 6.3367 95.585014 35-39
GTAAT 12246030 6.021162 40.13952 110-119
GGTGA 20070715 5.9588585 102.17061 45-49
CCCTG 19900120 5.9003105 67.68758 30-34
AATAC 8606150 5.576686 39.43804 110-119
AGACG 14111340 5.521419 103.3989 40-44
ACAGT 11655535 5.4043674 36.068142 110-119
GTAGT 15181985 5.341448 30.56977 80-89
GTGAC 15423135 5.1171803 85.162964 45-49
GAGAC 12554820 4.912391 143.76595 40-44
CCCCA 12503725 4.885858 102.56588 60-69
TAGTA 9697830 4.7682557 39.654446 80-89
CTCAG 12712040 4.7133656 34.73579 140-149
ACTCC 11199650 4.6406436 117.295166 30-34
GGCCC 18187355 4.550494 58.982243 60-69
ACCAG 10395710 4.545634 92.178375 45-49
GCACA 10136480 4.4322834 34.548473 110-119
TGACC 11895795 4.410719 146.32027 45-49
GTGTC 15602915 4.389746 23.811052 120-129
CGTGT 15495430 4.359506 23.262028 120-129
AGTAG 10481695 4.348969 31.95609 80-89
CACTC 10323040 4.277415 95.862 25-29
CTCCC 12453160 4.1262603 66.97103 30-34
CCCAG 11677585 4.0831623 87.61905 60-69
GACCA 9210955 4.0275874 105.73548 45-49
ATGCA 8677530 4.0235443 93.96525 25-29
TGAGG 12917165 3.8350184 69.52534 35-39
AGGAG 10928090 3.826211 95.92694 35-39
CCCTT 10889260 3.8260243 40.221813 20-24
ACGGT 11363005 3.770086 97.643745 40-44
TACAC 7255965 3.7598078 27.002893 110-119
GACGG 13421165 3.7576656 109.557625 40-44
AGATA 6424090 3.7249546 20.158203 150-159
ATGGT 10557475 3.7144156 26.256659 190-199
CTGAG 11173755 3.7072954 105.28786 35-39
TGTCC 11690020 3.6754186 20.687628 120-129
CAGGG 13034140 3.6493058 34.75837 50-59
GAGGA 10308830 3.6093917 126.0401 35-39
CCACT 8673530 3.5939305 28.261164 240-247
TGCAC 9520860 3.5301416 112.5481 25-29
GCCCC 12579185 3.5172188 73.372 60-69
TGGTG 13773945 3.4676416 18.213383 25-29
CTTGG 12290820 3.4579167 23.000687 20-24
AGGCT 10397135 3.4496243 28.851414 30-34
CCGTG 12853465 3.4102135 23.394306 120-129
GGGTT 13424170 3.3795846 41.157898 50-59
CCTGA 9078770 3.3662236 82.42285 30-34
GGATG 11280625 3.3491411 68.34579 20-24
CAGAT 7168465 3.32383 18.370062 150-159
CTCTC 9457045 3.3228045 13.851055 130-139
TCTTG 9959610 3.3205206 18.370672 170-179
CCAGG 10539080 3.2975278 39.445442 50-59
TCAGG 9905425 3.2864814 21.151861 140-149
GCACT 8755760 3.2464583 87.09093 25-29
CTTCA 7331730 3.2214582 17.49175 200-209
TCGCA 8613685 3.193779 23.785353 100-109
TCTCA 7229640 3.1766014 22.04416 130-139
CCTGG 11893785 3.155596 28.829699 50-59
TGTCT 9431200 3.1443498 19.97529 180-189
CGCAC 8871670 3.1020513 24.295092 100-109
ACAGA 5644300 3.086364 14.346023 140-149
CGGAT 9139065 3.0322142 77.61295 20-24
GGTTG 11844425 2.9818778 71.62394 15-19
TTCCC 8443885 2.9668229 48.550797 50-59
GCCCT 9997115 2.964107 21.475807 20-24
TGGCC 11138985 2.9553366 43.24373 60-69
GATGC 8788075 2.9157603 93.75844 25-29
GGTTC 10300190 2.89787 46.465683 50-59
TCTCT 7772520 2.8958998 15.642999 180-189
AGGGT 9694200 2.878142 49.002674 50-59
GATGG 9691230 2.8772602 22.154188 15-19
TCAAA 4379620 2.8379426 57.62432 70-79
CGGTG 11897825 2.8246918 68.54024 40-44
ACCAC 5746570 2.808063 19.514048 230-239
GACTC 7572745 2.80782 19.026134 190-199
GTTGG 10997395 2.7686348 51.43025 15-19
GCCGT 10425505 2.76604 22.154833 120-129
TTCTT 6976110 2.7561786 20.821781 170-179
TCACA 5281480 2.7366931 11.589981 140-149
CGGCC 10921395 2.7325437 12.475924 120-129
TCCAT 6193060 2.7211428 20.102577 70-79
GTTCC 8597610 2.7031446 52.639317 50-59
AGATG 6471945 2.6852803 17.645226 180-189
AGTCA 5765330 2.673233 30.765907 70-79
CCAGT 7146625 2.6498237 35.11626 60-69
TGGAG 8825585 2.6202562 19.564243 25-29
GGCTG 10981110 2.607052 14.931336 30-34
TCCTC 7237830 2.5430665 17.244053 130-139
TGTTC 7624150 2.5418816 13.273694 140-149
AGGGA 7131320 2.4968622 8.911736 150-159
GCGGA 8908520 2.4942124 84.93896 20-24
ACAGC 5697450 2.49127 11.60491 120-129
GAGAT 5926160 2.4588282 17.192228 180-189
AATAA 3029430 2.4548497 10.857712 110-119
GGCGG 11995200 2.4031496 40.7925 20-24
ACTCT 5454820 2.396771 13.591193 200-209
GTTCT 7123860 2.3750856 17.496891 160-169
CTGGA 7140120 2.3689916 15.123495 180-189
CATTT 5035930 2.3463788 18.334824 150-159
TTGGG 9286890 2.3380086 77.445786 15-19
TCATT 4993100 2.326423 16.959547 150-159
CACGG 7425730 2.3234048 9.423517 120-129
CTGGC 8748520 2.321111 33.995766 60-69
CAGCT 6205390 2.3008325 11.958208 150-159
TTCAT 4927635 2.295921 16.463066 140-149
TGTAG 6509685 2.2902896 7.891237 210-219
TACCA 4418305 2.2894237 16.28125 230-239
GTGAA 5454590 2.2631686 18.725487 190-199
CAGAG 5719300 2.2378209 9.238237 140-149
GATAC 4776555 2.2147639 14.962404 150-159
TTGTC 6639315 2.2135386 13.511335 170-179
TTCAC 5019625 2.2055523 19.252707 200-209
ATTTG 5251790 2.189616 16.134388 150-159
ACTAC 4147125 2.148907 12.78646 230-239
TCAGC 5776065 2.1416473 13.965388 140-149
GAATC 4617790 2.1411488 15.254341 190-199
CTACT 4859940 2.1353889 7.8386064 230-239
TACAG 4543770 2.1068275 14.13645 160-169
CTCGC 7100930 2.105399 16.13146 100-109
ATGTA 4250255 2.0897772 8.854927 70-79
CACTA 4027580 2.0869627 15.348089 240-247
TGAAT 4241600 2.0855217 14.72647 190-199
GTCCT 6630165 2.0845668 14.600946 130-139
GCTGT 7390675 2.079303 10.342262 140-149
CTCTG 6611320 2.0786417 15.005696 180-189
TTGGA 5889930 2.072242 15.758125 170-179
GTCTC 6578740 2.0683987 12.22875 180-189
ATATA 3005680 2.0652962 10.959599 110-119
GTTCA 5239370 2.0600007 15.402821 140-149
ACACA 3369050 2.0587437 14.000028 110-119
TCTGC 6547535 2.0585876 12.390346 210-219
TGGGG 9659195 2.0520437 50.891148 15-19
CAGGC 6549055 2.0491054 15.534433 140-149
TAGTC 5198770 2.0440376 25.119879 70-79
TCTCG 6485490 2.03908 15.742029 100-109
AATCG 4393115 2.0369728 13.847715 190-199
AATAT 2957630 2.0322795 13.660536 110-119
ATCTC 4616205 2.0282953 8.091846 100-109
TTGCA 5150865 2.0252023 14.578824 150-159
GAACT 4351870 2.0178485 10.709824 160-169
AGCTC 5414195 2.0074735 11.956198 140-149
TTTGC 5998685 1.9999535 12.406369 150-159
TGGAA 4775065 1.9812262 9.313491 170-179
GTCAA 4267125 1.9785545 39.25424 70-79
ACGGC 6302850 1.9720714 14.28961 120-129
AACCA 3219805 1.9675441 14.919063 70-79
CCAGA 4463360 1.9516515 29.784885 60-69
GATAT 3965320 1.9496795 7.978331 180-189
GAGTC 5849305 1.9407177 12.854506 210-219
CCTTC 5500875 1.9327743 14.338838 200-209
CAGCC 5495495 1.9215442 10.545324 120-129
TCGGC 7234830 1.9195067 7.899453 130-139
ACACG 4386125 1.9178795 13.232735 120-129
ATATG 3858130 1.8969761 8.214011 180-189
TACTA 3424385 1.8815918 10.438303 240-247
CATAG 4037570 1.8721157 8.49451 210-219
GACGT 5639315 1.871046 9.488158 170-179
CCTTG 5906300 1.8569793 26.174925 20-24
GGGGC 9159725 1.8350831 46.293423 15-19
CTGTT 5473690 1.8249209 12.994856 140-149
AGTAT 3659915 1.7995172 12.414042 220-229
GGGCG 8934550 1.7899711 57.028496 20-24
GTTGT 5965455 1.7797115 6.915595 210-219
GCAGA 4545120 1.7783933 14.778079 150-159
AGAAC 3226035 1.7640303 11.39712 160-169
CGTAG 5290500 1.7553139 9.927553 210-219
ATTGT 4201670 1.751792 8.229159 50-59
TGCAG 5266650 1.7474008 13.685541 150-159
AGTCT 4363400 1.7155892 13.6129875 210-219
GTCCC 5734605 1.7002888 22.247414 50-59
GGTCA 5112330 1.6961995 7.5151196 130-139
CAAAA 2204115 1.6843249 22.42393 70-79
ATAGT 3422985 1.6830227 7.6290383 220-229
CTCCA 4048110 1.677359 7.6926703 150-159
GTCAC 4515785 1.6743613 8.443494 140-149
TCAGA 3582680 1.661195 10.199774 130-139
TTGGT 5561070 1.6590686 27.484892 25-29
CACCA 3390815 1.6569226 13.646915 230-239
TGACT 4193575 1.6488179 11.573873 190-199
AACTG 3545250 1.64384 10.602223 160-169
TCCCA 3943115 1.6338537 11.075742 240-247
GAGAA 3330040 1.6294035 10.543456 160-169
TGGTT 5432570 1.6207325 17.618528 10-14
AATAG 2790790 1.6182163 9.64264 240-247
TCTGG 5726055 1.6109763 12.22479 180-189
TTCAG 4048940 1.5919505 9.228171 150-159
CTGTG 5617885 1.5805436 5.3828444 210-219
CAGAC 3614375 1.5804235 17.94187 60-69
GAACC 3595400 1.5721266 12.208388 70-79
ATAGA 2706700 1.5694573 10.257598 240-247
GTCTG 5569295 1.5668734 9.293426 210-219
ACCAT 3019715 1.5647192 12.577934 45-49
GCTCC 5259170 1.5593241 6.1890545 150-159
GGCCG 6935000 1.5526645 12.880353 120-129
ACTGA 3345245 1.5511028 8.523785 180-189
GCTGA 4658215 1.5455306 24.494816 30-34
TGTGC 5486635 1.5436176 8.141112 220-229
TGCTC 4906410 1.5426073 5.329163 150-159
CTGGT 5478305 1.541274 6.7691555 160-169
TTGTA 3685985 1.5367886 8.907607 210-219
CAAAG 2802170 1.5322567 28.024666 70-79
GCTCA 4116410 1.5262812 10.718572 140-149
GGACG 5422855 1.5182941 7.116617 170-179
CCTCG 5100190 1.5121871 9.828185 130-139
TAGTT 3626540 1.5120044 9.675071 220-229
GTGCT 5343425 1.5033268 7.853988 220-229
GCTCT 4712380 1.4816029 9.964182 130-139
CATGT 3766385 1.4808564 8.815498 70-79
AAATA 1827235 1.4806701 5.0354624 9
CCCAC 3775910 1.4754453 10.646211 240-247
CCACC 3763235 1.4704925 9.750679 230-239
CGACT 3832375 1.420967 8.392368 190-199
ACCGT 3811335 1.4131656 21.018505 45-49
GTGGA 4755685 1.4119304 24.636793 25-29
AATTG 2855270 1.4038872 9.3148155 170-179
TCGAC 3766295 1.3964655 9.096104 190-199
TATAC 2536555 1.3937571 6.842709 110-119
CCATC 3348140 1.3873224 11.378791 240-247
CCTCA 3334310 1.3815918 7.4390283 130-139
TGGAC 4154495 1.3784033 10.223708 170-179
CTCTT 3685095 1.3729994 8.025145 200-209
TGATA 2790430 1.3720063 7.450945 180-189
CACTG 3691285 1.3686535 7.211629 230-239
GTCCA 3656840 1.355882 18.408144 70-79
CCATG 3638420 1.3490524 8.917331 70-79
GCGTA 4062790 1.3479768 10.567895 210-219
ACGGA 3427420 1.3410648 9.654075 200-209
GGGAG 5307630 1.3297521 6.352949 150-159
AAAAA 1388940 1.3273082 8.051476 8
GTAGG 4434730 1.3166411 5.131947 160-169
GAATT 2671730 1.3136436 10.359422 170-179
ACTTC 2986155 1.3120743 6.9816656 220-229
AGCCG 4190750 1.3112259 9.3902235 120-129
CTCGA 3519275 1.3048755 8.098495 190-199
CTGCT 4136585 1.300569 5.4694495 90-99
AGATC 2803550 1.299933 6.8490167 130-139
GAGGG 5174860 1.2964885 7.9422536 200-209
TATGG 3683020 1.2957896 6.028891 190-199
GGAGG 5162440 1.2933768 18.573912 30-34
CTGAT 3257710 1.280857 7.0403485 180-189
GTGTT 4278345 1.2763854 7.697942 160-169
ATCGG 3842330 1.2748313 8.196163 190-199
TTGGC 4522125 1.2722609 8.844471 60-69
TCCAC 3063460 1.2693633 9.558842 240-247
CTTGA 3202415 1.2591163 8.181534 200-209
TGGTC 4472450 1.2582854 13.557937 50-59
GGAGT 4236890 1.2579039 7.221566 210-219
ACGTG 3783515 1.2553173 9.106801 170-179
AAGTA 2156035 1.2501588 15.644722 70-79
GGCTC 4706325 1.2486572 8.347492 130-139
GTGGT 4944710 1.2448491 11.959595 50-59
ATAAA 1532100 1.2415124 5.723568 7
ACTAT 2246205 1.234219 6.5191584 230-239
ATAAC 1897000 1.2292341 7.456313 80-89
GATCT 3097320 1.2177955 5.3995237 130-139
GGAAT 2923055 1.2128073 8.655925 170-179
TTCCA 2744115 1.2057252 7.826697 230-239
AAAGT 2077195 1.2044439 20.519278 70-79
TGCCC 4052920 1.2016755 7.5704985 60-69
AGTTC 3041980 1.196037 5.657425 160-169
ACTCG 3223095 1.1950581 8.323972 190-199
CCATA 2276235 1.179472 11.349413 70-79
ACTGG 3549435 1.1776528 6.9893064 160-169
GCATA 2529415 1.1728237 8.117768 210-219
ATACC 2257280 1.1696501 8.23343 70-79
TTGAG 3322885 1.1690838 7.8743997 200-209
TATCA 2122235 1.1661013 7.368807 70-79
CAATA 1779230 1.1529202 6.795592 100-109
AGAGT 2760175 1.1452266 5.0186405 210-219
ACTGT 2902450 1.1411769 5.501857 230-239
ATGGG 3841560 1.140533 34.931187 15-19
TCACG 3074830 1.1400844 9.722631 200-209
AGCTT 2893565 1.1376836 5.983439 150-159
AAAAG 1639200 1.1208969 15.100405 70-79
AGGTT 3152795 1.1092417 22.88412 10-14
TCTGT 3312600 1.1044164 6.6134253 210-219
AGAGC 2816795 1.1021423 6.7304354 140-149
GAAGA 2242135 1.0970867 19.311066 35-39
CTCGG 4131010 1.0960176 8.396305 130-139
CGGCT 4127760 1.0951556 7.880995 130-139
AGAGA 2233580 1.0929008 14.292815 35-39
ATATC 1972025 1.0835657 7.8455153 70-79
GTCTA 2746425 1.0798316 7.316017 180-189
TCTAC 2444510 1.0740831 7.111893 180-189
CTGAA 2268440 1.0518163 15.809429 35-39
ATCCA 2027890 1.0507874 15.591362 240-247
AACTT 1898900 1.0433859 6.0086856 210-219
TATGT 2485935 1.0364548 7.704211 220-229
GAGCT 3122905 1.0361363 5.9937186 140-149
GTCCG 3897785 1.0341398 5.016133 130-139
TACTG 2628235 1.0333619 6.1690817 180-189
CTGCC 3469715 1.0287573 5.860327 200-209
GCTTC 3270175 1.0281644 5.306375 150-159
CATGG 3072380 1.0193727 5.3417993 190-199
CTGCG 3834815 1.0174328 7.017556 210-219
GCGGT 4274355 1.0147852 5.6658564 130-139
ATACG 2160515 1.0017744 5.1830516 120-129
AAAAT 1234265 1.0001665 5.2668 8
TATAA 1436415 0.9870053 6.7451963 240-247
TCCCC 2976300 0.9861744 5.290274 80-89
ACAAT 1518685 0.98409027 7.8254 100-109
TAGAT 2000785 0.9837515 7.8563104 240-247
CGAAC 2240975 0.97989 12.791829 70-79
CTACC 2360350 0.9780254 8.315727 230-239
TGCGT 3458585 0.9730432 8.347861 210-219
CGTGG 4081620 0.9690274 8.191166 50-59
CCATT 2190175 0.96233207 6.21328 50-59
CTTCT 2572155 0.95833826 5.3752565 210-219
GGGGT 4506845 0.9574548 7.29409 10-14
TAGGC 2860970 0.9492298 5.769593 160-169
GAGTT 2696720 0.9487817 5.3608646 160-169
CAGCG 3024795 0.9464151 6.274322 160-169
ACGTC 2545405 0.9437845 12.555276 70-79
CGGAG 3368410 0.9430894 6.7285957 210-219
AAGAG 1925315 0.9420652 14.382092 35-39
TGAAG 2269100 0.94147426 10.471803 35-39
GAGGC 3354070 0.93907446 15.879232 30-34
ACCCA 1874065 0.91576236 12.943532 240-247
CCCAA 1869020 0.9132972 9.546872 240-247
GGTGG 4297060 0.9128871 24.306183 25-29
TCGAA 1944790 0.9017483 16.552023 70-79
ATCAA 1380535 0.8945706 13.993824 70-79
TGCAT 2264990 0.89054227 6.149299 210-219
TGTAA 1808245 0.8890829 6.656741 230-239
GGGCC 3962060 0.8870585 18.375677 15-19
CATCA 1699460 0.88060564 5.962444 230-239
ACTAA 1354080 0.87742805 12.624613 240-247
CGTCC 2867555 0.85021925 10.221835 70-79
TTCTG 2542700 0.8477328 5.3936396 210-219
CGGTC 3180375 0.84380025 5.3916316 130-139
CCAAT 1626985 0.8430514 10.529797 240-247
TGGGC 3524620 0.8367887 19.974762 15-19
AGCGT 2507815 0.832058 6.459515 160-169
GACCG 2652335 0.8298778 19.324821 45-49
CCAGC 2347625 0.8208661 5.5346494 80-89
TGAAC 1770315 0.8208489 6.511034 190-199
GGTCC 3069735 0.81444585 13.675285 50-59
TAACC 1519595 0.7874054 5.92578 80-89
CTTCC 2232500 0.78440577 5.439559 230-239
GTATG 2225295 0.78292114 5.3279266 220-229
AACCG 1783085 0.7796728 5.800263 190-199
TGAGA 1818225 0.75440127 5.8791256 240-247
AAAGG 1529160 0.7482248 8.816271 10-14
CATAC 1422570 0.73713005 9.771125 70-79
AGCCA 1684280 0.73646927 5.7340193 80-89
AAAGA 1075695 0.7355681 5.892169 8
GTCGA 2180185 0.7233549 11.569736 70-79
CCTTT 1935725 0.7212161 5.20605 50-59
AATCC 1372320 0.7110922 9.279421 240-247
CTAAT 1293635 0.71081173 9.834358 240-247
GTAGC 2139910 0.7099922 5.475218 80-89
AAAGC 1284600 0.702433 10.266032 70-79
ATGAT 1422370 0.6993548 6.922199 240-247
AAGGT 1670380 0.6930588 9.883339 10-14
CATTG 1738930 0.6837075 5.6885347 50-59
TGTTA 1615575 0.6735778 5.0049696 230-239
CGATG 2025605 0.67206746 15.185908 15-19
AGGAT 1604970 0.6659195 6.6096334 10-14
GGAAG 1845355 0.6461072 6.6161013 30-34
TAGGT 1827205 0.6428619 5.323621 10-14
CCGTA 1708870 0.6336143 5.3688097 80-89
AGAAG 1288860 0.6306449 5.572901 9
GAGGT 2114670 0.62783116 6.6822624 10-14
ATAGG 1491795 0.61896193 5.2089853 10-14
TATGA 1258710 0.618886 5.816046 240-247
CAATC 1179315 0.61108315 9.003694 240-247
AGACC 1390980 0.6082207 5.052086 240-247
AATGG 1380845 0.5729276 6.6335034 10-14
CATCC 1356225 0.5619601 7.4764705 240-247
TAAAA 692650 0.56127775 5.598414 8
AAATG 965735 0.5599733 5.497923 9
GGGTC 2311785 0.5488466 5.5145216 70-79
AAGAA 782620 0.53516126 5.5604825 8
AAGCA 975380 0.53334826 6.8842773 70-79
GAAGG 1499255 0.5249285 5.480928 10-14
AAGGG 1448930 0.50730836 6.1894736 10-14
GAAAA 739435 0.505631 5.9744735 8
ACGAT 1070935 0.49656457 6.7858496 10-14
TGATC 1226020 0.48204306 5.3993154 240-247
GGTCG 2028245 0.4815306 5.580329 70-79
TTGCC 1530335 0.48114726 5.3486075 60-69
ATAAG 828660 0.48049158 5.945965 9
GATCC 1248045 0.46274975 5.4082494 240-247
CGGTT 1575310 0.44319993 7.8257966 10-14
TAAGG 1058850 0.43932837 5.9621434 10-14
GAAGC 1120545 0.4384416 5.4132266 30-34
TACCG 1167935 0.43304658 5.1442924 70-79
AAGCT 910880 0.42235127 9.0691185 30-34
TAAAG 693945 0.40237823 5.698914 9