FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_5_SL254632.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_5_SL254632.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 26769015
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 31393235 8.316704 56.52498 110-119
CACAG 40198160 7.1964984 41.192844 110-119
CAGTA 34248100 6.4074244 41.490707 60-69
TCCCT 42445820 6.380729 89.798836 30-34
ATACA 24712830 6.264778 29.481714 110-119
GGAGA 43410355 6.175743 92.43321 35-39
AGTAA 25955665 5.8655095 42.69376 110-119
GGTGA 47026055 5.78815 93.94718 45-49
CCCTG 42681270 5.4730716 53.40894 30-34
GTAAT 27919100 5.4585886 36.45863 110-119
GGCCC 47500450 5.1957707 59.525734 60-69
AGACG 31839200 5.0812154 102.16712 40-44
ACAGT 27035765 5.0580797 34.232555 110-119
CCCCA 29502815 4.905254 104.83387 60-69
GTAGT 33471085 4.8296156 24.453344 70-79
GTGAC 34924470 4.8221493 84.627625 45-49
GAGAC 29904415 4.7724433 133.68869 40-44
GTGTC 36945540 4.41345 24.582808 120-129
ACCAG 24586150 4.4015493 96.65188 45-49
CCCTT 28824020 4.3330126 68.49889 20-24
AATAC 17070585 4.3274455 29.589577 110-119
TGACC 27550480 4.267265 154.2807 45-49
CGTGT 35406680 4.2296205 23.399569 120-129
TAGTA 21387600 4.1815863 28.083994 80-89
GCACA 23299445 4.1711965 35.297714 110-119
GACCA 23032885 4.123475 108.37748 45-49
TGGTG 37570775 4.000891 30.35271 25-29
ACTCC 22868995 3.9735353 89.95602 30-34
CCCAG 26779775 3.9691305 86.01092 60-69
AGTAG 23321020 3.8894212 27.417173 70-79
CTCAG 25055510 3.8808215 30.025362 140-149
TGAGG 30732595 3.7826877 70.41874 35-39
CTTGG 31499490 3.7628744 39.44059 20-24
CCACT 21621750 3.756824 27.101656 240-247
GACGG 31728705 3.7369878 113.06115 40-44
GCCCC 30037075 3.6856956 76.99545 60-69
CTGAG 26451740 3.6522884 99.16521 35-39
CACTC 21019570 3.652194 74.685135 25-29
CAGGG 30963930 3.646913 34.680054 50-59
AGGAG 25611015 3.643533 91.990456 35-39
CTCCC 24983410 3.5938103 56.509388 30-34
ACGGT 25958305 3.5841584 92.90315 40-44
TGTCT 25405460 3.5578265 21.886045 180-189
ATGGT 24525530 3.5388422 26.071302 190-199
AGGCT 25586005 3.5327532 48.223503 30-34
GCCCT 27271895 3.4971085 36.630154 20-24
TCTTG 24967640 3.4965138 20.035946 170-179
AGATA 15209230 3.4370103 18.825966 150-159
GAGGA 24108305 3.4297507 129.82104 35-39
TGTCC 25272325 3.386655 18.795364 120-129
CCAGG 25303190 3.343137 40.34238 50-59
GATGG 26847275 3.3044674 35.411713 15-19
CTCTC 21559960 3.241032 13.6547165 100-109
CTTCA 17819880 3.2356887 17.612417 200-209
CCGTG 28123210 3.2147644 23.986265 120-129
TCAGG 23273455 3.213451 20.267944 140-149
TTCCC 21242045 3.193241 56.061836 50-59
CCTGG 27610410 3.156146 32.089252 50-59
ACAGA 14398410 3.1135523 13.966233 140-149
GGGTT 29020385 3.0903652 43.00656 50-59
ATGCA 16486370 3.0844097 74.90646 25-29
TGGAG 25044225 3.082541 33.55344 25-29
TCGCA 19863910 3.0767 23.872103 100-109
TCTCT 19390755 3.0462253 17.19751 180-189
CGCAC 20422665 3.0269194 24.0555 100-109
TCTCA 16384180 2.974998 20.107819 130-139
TGGCC 25756065 2.9441755 40.31913 60-69
GGTTC 24623615 2.9414942 48.70178 50-59
GGATG 23814255 2.9311514 52.45952 20-24
AATAA 9505985 2.9107637 14.0615425 110-119
TACAC 13832815 2.9031348 21.103962 110-119
GACTC 18711315 2.8981757 18.929976 190-199
CCAGT 18553410 2.873718 41.294964 60-69
TGCAC 18541680 2.8719013 96.6505 25-29
TTCTT 17290720 2.8386536 22.510975 170-179
TCACA 13489890 2.8311636 12.129476 140-149
GTTCC 20987850 2.8125076 55.723988 50-59
GCCGT 24369130 2.785635 22.846128 120-129
CAGAT 14887985 2.7853706 15.967833 150-159
CGGTG 27284685 2.7803123 67.44966 40-44
AGGGT 22526610 2.772663 51.078556 50-59
TCAAA 10898595 2.7628274 57.28262 70-79
CCTGA 17597205 2.7256124 62.809055 30-34
CGGCC 24829415 2.715931 11.562933 200-209
ACCAC 13468750 2.7049046 15.381251 230-239
GGCTG 26433270 2.693553 26.752832 30-34
AGATG 15796820 2.634554 17.242306 180-189
GCACT 16979465 2.6299314 67.3557 25-29
CGGAT 18957930 2.6175907 59.671078 20-24
AGGGA 18038370 2.5662158 9.020728 150-159
GGTTG 24004655 2.5562425 55.462383 15-19
TGTTC 18193310 2.547824 13.212681 150-159
AGTCA 13549735 2.5349991 29.686354 70-79
GAGAT 15138270 2.5247226 17.046091 180-189
CTGGC 21748840 2.4861097 35.881046 60-69
GTCTC 18258195 2.4467165 13.885886 180-189
TCATT 12875415 2.4431787 18.130735 150-159
TTGTC 17251150 2.4158826 15.033913 170-179
CATTT 12714210 2.412589 19.338015 150-159
ACAGC 13241520 2.3705707 12.791574 120-129
TTCAT 12484895 2.3690753 17.512184 140-149
GTTGG 22152075 2.3589623 43.173706 15-19
CTGGA 16964655 2.342372 16.035889 180-189
GATGC 16958225 2.341484 71.45964 25-29
GTTCT 16667610 2.3341622 17.458 160-169
TCCTC 15516860 2.3325942 16.075752 130-139
ACTCT 12831335 2.3298814 12.654384 200-209
CAGAG 14453805 2.3066819 8.759711 140-149
TCCAT 12697020 2.3054926 13.225813 70-79
AATAT 8694015 2.3032207 13.21597 110-119
ATATA 8632140 2.2868288 10.892152 110-119
TTCAC 12457870 2.2620685 18.157263 200-209
GTGAA 13533790 2.2571316 18.039173 190-199
TTGGT 17939155 2.2394953 43.823124 25-29
CACTA 10517630 2.2073667 15.487398 240-247
ATTTG 13007040 2.2002034 16.604174 150-159
TGTAG 15212905 2.195103 7.946224 70-79
GAATC 11732320 2.1949818 16.146511 190-199
TGAAT 11154350 2.1808372 15.583165 190-199
CTCTG 16237640 2.1759489 15.993896 180-189
GAGTC 15688270 2.1661367 13.81003 210-219
TAGTC 13302135 2.1531475 24.580097 70-79
GGCGG 24597495 2.13808 34.894176 20-24
ACTAC 10157095 2.1317003 12.436617 230-239
TCGGC 18537910 2.1190684 8.826397 190-199
TTGGA 14623635 2.1100762 16.953005 170-179
TCTGC 15745275 2.109969 11.2576 210-219
CCTTG 15738890 2.109113 44.839317 20-24
AATCG 11188845 2.093304 14.258629 190-199
CAGCC 14095910 2.0892076 11.567816 120-129
GTTCA 12866560 2.0826428 15.779019 140-149
GTCAA 11104675 2.0775566 40.48335 70-79
GATAC 11091290 2.0750525 13.320952 150-159
ACACA 8520380 2.066856 13.771459 110-119
CTCGC 16096070 2.0640192 16.222477 100-109
GCGGA 17444125 2.054558 73.29222 20-24
CCTTC 13619560 2.04738 14.35255 200-209
AATAG 8931450 2.0183458 7.9931464 110-119
ATATG 10322835 2.0182638 8.654162 180-189
TACAG 10773710 2.015637 12.533361 160-169
GCTGA 14579935 2.013105 41.800293 30-34
TCTCG 15007340 2.0110807 15.951231 100-109
CACGG 15209725 2.0095565 7.987853 200-209
CTACT 11021790 2.0013087 8.460512 230-239
TTGCA 12333805 1.9964088 14.367772 150-159
TTTGC 13979205 1.9576731 12.616989 150-159
ATAGA 8648115 1.9543172 9.38051 240-247
TGGAA 11653840 1.9435982 9.665752 170-179
GTCCT 14459640 1.9376854 13.877891 130-139
ATTGT 11403985 1.9290389 8.817135 170-179
GCTGT 16129500 1.9268019 10.3951 140-149
GTTGT 15228295 1.9010757 7.023541 210-219
CAGGC 14326120 1.892812 14.900217 140-149
AGTCT 11681360 1.890801 13.914665 210-219
TACTA 8619460 1.890465 10.777654 230-239
AGTAT 9655300 1.8877511 12.277149 220-229
GATAT 9620475 1.8809423 8.523031 180-189
GGTCA 13558150 1.8720233 9.750679 140-149
TACCA 8912045 1.8703977 12.080755 230-239
TTGGG 17544220 1.8682746 67.29598 15-19
TCCCA 10675950 1.8549685 11.599224 240-247
CTGTT 13103635 1.835057 12.795439 140-149
CATAG 9670735 1.8092829 7.5856633 210-219
GTCAC 11568325 1.7918056 9.460172 140-149
GCAGA 11213400 1.7895455 14.372075 150-159
TCTGG 14878615 1.7773736 13.039934 180-189
GTGGA 14388755 1.771024 41.179245 25-29
ATAGT 9046815 1.7687835 7.464289 220-229
AAAAA 4987590 1.7652051 6.473746 8
GTCTG 14670535 1.7525166 9.251983 210-219
ATGTA 8946005 1.7490739 11.273201 70-79
ATAAT 6591945 1.7463398 6.0341964 110-119
GAACT 9293765 1.7387562 10.44111 160-169
TGCAG 12401485 1.7123184 13.509263 150-159
ATAAA 5587880 1.7110271 6.9142337 110-119
AGAAC 7861070 1.6998998 10.819581 160-169
TTGTA 10009330 1.6931263 9.286495 220-229
GGAGG 16049050 1.6850353 29.621178 30-34
ACGGC 12659825 1.6726558 12.287901 120-129
CACCA 8307590 1.6683981 11.233159 230-239
TGGGG 18252220 1.6579853 39.95493 15-19
TCAGC 10689120 1.6556265 10.417318 150-159
CAAAA 5637400 1.651797 21.066833 70-79
AACCA 6786205 1.6461835 12.075554 70-79
ATCTC 8981995 1.6309279 6.996266 100-109
GACGT 11761205 1.6239127 9.389899 170-179
GTCCC 12622420 1.6185883 17.925474 50-59
ACGGA 10128985 1.616484 10.253978 200-209
CCAGA 9027560 1.6161641 24.339844 60-69
ATGGG 13089725 1.6111344 56.882957 15-19
CCCAC 9644710 1.6035674 10.992285 240-247
CAGCT 10323400 1.5989805 8.718753 150-159
GAGAA 8294090 1.5988246 10.800199 160-169
TTCAG 9873485 1.5981697 9.691012 150-159
CAAAG 7350170 1.5894213 29.520462 70-79
GGACG 13477955 1.5874251 7.5028644 170-179
TGACT 9764640 1.5805513 11.088569 190-199
AACTG 8429000 1.5769687 10.182117 160-169
CGACT 10144530 1.5712756 9.343442 200-209
AAATA 5106670 1.5636791 6.337022 6
CCTCG 12183810 1.5623449 10.002387 130-139
GGGGC 17867585 1.5530982 37.377235 15-19
TCGAC 10013335 1.5509549 9.360867 190-199
CTCTT 9745160 1.5309334 8.726891 200-209
CACTG 9841910 1.5244031 6.173439 230-239
AGCCG 11401735 1.506433 11.160905 120-129
GGGCG 17231285 1.4977893 45.324657 20-24
TGGTT 11979290 1.4954752 15.461708 10-14
CTCCA 8595860 1.4935485 7.835355 240-247
GCTCT 11120305 1.4901928 9.383568 130-139
AATTG 7614125 1.4886718 9.817303 170-179
ACTGA 7917050 1.4811888 6.9991508 180-189
TCAGA 7794110 1.458188 8.093194 130-139
ATCGG 10541290 1.4554745 9.834459 190-199
AGCTC 9386735 1.4539014 8.680527 140-149
CAATA 5723840 1.4510111 7.6825695 100-109
GGCCG 14770575 1.4402572 11.726095 120-129
GAATT 7342720 1.4356081 11.028342 170-179
CCATC 8197850 1.4243934 11.1647415 240-247
CTCGA 9195840 1.4243339 8.434326 190-199
TCCAC 8185155 1.4221877 9.664773 240-247
CCACC 8539460 1.4198041 8.330469 230-239
CTGGT 11742300 1.4027147 6.5251184 160-169
ACCAT 6640500 1.3936619 10.689273 45-49
CGTAG 10067080 1.3899986 8.242414 210-219
TGGAC 10040135 1.3862782 10.479206 170-179
CAGAC 7716220 1.3814007 12.342339 60-69
GAGGG 13109510 1.3764048 8.452239 200-209
GAGGC 11683795 1.3761101 26.23599 30-34
ACTAT 6218950 1.3639727 7.058838 230-239
AAGTA 5967355 1.3485141 17.813177 70-79
ACACG 7501480 1.3429567 9.03724 120-129
GGGCC 13767730 1.3424712 32.43664 15-19
GGCTC 11724600 1.3402389 8.5415945 130-139
TTCCA 7331295 1.331198 8.059922 230-239
CTTGA 8207240 1.3284632 8.769924 200-209
TAGTT 7846530 1.3272783 8.312877 220-229
GGGAG 12632095 1.3262795 6.345005 150-159
GGTGG 14577655 1.3241972 41.409637 25-29
CATGT 8156720 1.3202857 10.738358 70-79
GTCCA 8410925 1.3027593 12.336161 70-79
TGGGC 12731800 1.2973716 34.284233 15-19
TATAC 5914280 1.2971509 6.468136 120-129
TATGG 8987425 1.2968152 6.4674997 190-199
GGAGT 10509440 1.2935429 7.550666 210-219
TATCA 5857335 1.2846613 5.9149 70-79
AAAGT 5674690 1.2823771 21.54636 70-79
CCATG 8273480 1.2814705 10.923769 70-79
CCTCA 7338340 1.2750518 6.1089454 130-139
AGAGA 6558815 1.2643213 13.149437 35-39
ACTCG 8154790 1.2630868 8.640292 190-199
ACGTG 9140780 1.2621009 8.887319 170-179
GTGTT 10097990 1.2606168 7.5272894 160-169
ACTTC 6941295 1.2603828 5.781817 220-229
GAACC 7038450 1.2600625 8.995777 70-79
AGAGT 7551020 1.2593402 5.1993856 210-219
ATCAT 5615060 1.2315243 5.424487 90-99
GGAAT 7381030 1.2309896 8.830212 170-179
GTGCT 10287790 1.2289615 6.8374023 220-229
GCGTA 8790450 1.2137296 9.402761 210-219
ACAAT 4785040 1.2130222 8.633827 100-109
CAGTG 8743260 1.2072139 5.155823 160-169
TCACG 7781585 1.2052815 9.046813 200-209
TGATA 6152790 1.2029597 6.1941576 180-189
TTGAG 8310940 1.1992036 7.902688 200-209
AGTTC 7359940 1.1913152 5.965595 160-169
CTGCG 10389810 1.187659 6.5689573 210-219
GCTCA 7640380 1.1834104 8.343298 140-149
AGTGA 7000045 1.16745 5.04172 160-169
TGTGC 9726785 1.1619447 6.0646963 220-229
TGCCC 9004500 1.154658 10.089831 60-69
CTGAT 7079590 1.1459363 5.664525 180-189
GCATA 6033655 1.1288272 7.3974323 210-219
ACTGG 8039420 1.1100321 6.575786 160-169
TATGT 6550120 1.1079843 7.315064 220-229
CGGCT 9657155 1.1039095 7.7714524 130-139
AAAAG 4220780 1.1024553 13.533303 70-79
CTCGG 9624065 1.1001269 7.9143777 130-139
ATCCA 5200120 1.0913649 14.290993 240-247
GTCTA 6677535 1.0808579 7.235763 180-189
GCGGT 10537900 1.073813 6.597837 130-139
TTGGC 8975690 1.0722203 5.1958146 170-179
ATAAC 4228710 1.0719908 7.245748 80-89
TATAA 4017065 1.064202 7.6466107 240-247
CTGAA 5640375 1.0552491 14.652028 35-39
TGGTC 8825050 1.0542251 8.774937 50-59
CCCAA 5229460 1.0502229 8.75533 240-247
CGATG 7560175 1.043861 24.517046 15-19
GTGGT 9774545 1.0408859 7.6520605 50-59
CATCA 4949670 1.0388021 6.4962683 240-247
TCTAC 5715465 1.0377997 6.7871485 180-189
AGGTT 7124060 1.0279461 16.695719 15-19
CGGAG 8692725 1.0238237 7.190384 210-219
GAAGA 5307025 1.0230179 18.703932 35-39
TAGAT 5202705 1.0172043 7.4310336 240-247
CCAAT 4840995 1.0159943 10.050073 240-247
AAAAT 3263660 0.9993434 5.9808903 5
CCATT 5485820 0.9961013 5.0778837 50-59
CGGTC 8643130 0.9879961 6.7070165 130-139
GAGTT 6711920 0.96847755 5.277756 160-169
ACCGT 6237905 0.96618253 13.668003 45-49
ATATC 4374980 0.9595434 5.9894 70-79
ATACG 5120215 0.9579332 5.0789824 120-129
TGTAA 4876685 0.95346254 6.1685586 230-239
AGAGC 5939775 0.9479282 5.48436 140-149
AAGAG 4912005 0.94687116 12.986064 35-39
TAGAC 5034240 0.9418482 5.0332747 110-119
GCTTC 6918465 0.92711914 5.096777 150-159
ACTAA 3652835 0.9260049 11.564818 240-247
ATCAA 3616730 0.9168522 13.510555 70-79
TGAAG 5438505 0.9070202 10.343435 35-39
CTACC 5157905 0.8961967 6.7297773 230-239
AGCTT 5523430 0.89404887 5.331534 150-159
CACAA 3665170 0.8890893 5.4431143 110-119
CTTCC 5896990 0.8864735 5.5901923 230-239
TGCGT 7155125 0.8547388 6.8163652 210-219
CCTTT 5390535 0.84683573 6.3641405 50-59
TGCAT 5207815 0.84296185 6.0428987 210-219
AGCTG 6036120 0.83342916 7.668756 30-34
ACCCA 4136635 0.83075285 11.231707 240-247
TACAA 3261755 0.8268649 5.1720824 100-109
AGCCA 4560880 0.81651413 7.844731 80-89
CCAGC 5505020 0.81591964 5.616231 80-89
TAACC 3788890 0.79518574 6.617348 80-89
AAAGA 3014490 0.78737587 5.225477 70-79
CGTTG 6439480 0.76924914 5.0171084 170-179
TGAAC 4108215 0.7685996 5.406238 190-199
AAAGG 3957660 0.7629052 7.5473466 10-14
TCGAA 4017405 0.75161016 13.164171 70-79
TGAGA 4496970 0.7499934 5.6656055 240-247
AGGAT 4495465 0.7497423 9.158336 10-14
GGAAG 5234605 0.7446974 10.385338 30-34
CTAAT 3338970 0.73232037 9.246593 240-247
AATCC 3486685 0.73176104 8.965632 240-247
ACGAT 3892680 0.7282756 9.229093 10-14
GGTCC 6360785 0.72710127 9.957921 50-59
GAAGT 4315385 0.71970904 5.4476447 70-79
ACGTC 4638415 0.7184392 7.9040647 70-79
ATACC 3392080 0.711906 5.664885 240-247
TAGGT 4928730 0.7111772 5.568767 10-14
GCGTT 5813475 0.6944676 5.078049 170-179
GTCGA 5023815 0.6936565 8.846851 70-79
GACCG 5209210 0.68825716 14.150446 45-49
CGAAC 3784285 0.67748374 8.929793 70-79
CAATC 3206545 0.6729673 8.973863 240-247
TGGAT 4623350 0.66711324 6.961957 10-14
AATGG 3993295 0.6659917 5.460688 10-14
CTTTG 4718550 0.6607944 5.5004315 50-59
AAAGC 3031960 0.6556395 9.018721 70-79
TTTCG 4636580 0.6493151 5.0954704 100-109
CGTGG 6313835 0.64338046 5.2616944 50-59
CGTCC 4875445 0.62518424 6.407699 70-79
AAGTC 3298550 0.6171206 5.0367866 70-79
GAAGC 3828045 0.6109174 9.490511 30-34
AAGAA 2329805 0.6085382 5.1875925 7
GATGA 3641495 0.60731936 5.171973 45-49
ATAGG 3594065 0.5994091 5.4364724 10-14
AAGGT 3587125 0.5982517 5.986017 15-19
TTGCC 4384225 0.5875146 6.5998483 60-69
AAGCT 3028700 0.56663483 15.439745 30-34
CATCC 3232600 0.56167096 6.5344024 240-247
GACGA 3481380 0.5555932 7.529591 40-44
TCAAG 2870460 0.53703 5.7400184 70-79
AAGCA 2288255 0.49481866 5.815671 70-79
GGGAT 3984605 0.49044073 6.3117576 10-14
CGGTT 3913110 0.4674533 8.778845 10-14
TCGAT 2740650 0.44361472 6.9005446 10-14
GCGAT 2558180 0.35321727 6.0072613 10-14