FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_2_SL254629.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_2_SL254629.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2439765
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2743620 8.032567 56.028095 110-119
CACAG 3578525 6.927478 39.767292 110-119
TCCCT 3911640 6.466017 93.23722 30-34
CAGTA 3100745 6.347931 41.861694 60-69
ATACA 2231030 6.1764765 30.463566 110-119
GGAGA 3834220 5.9417076 89.47599 35-39
GGTGA 4201360 5.6907353 90.711876 45-49
AGTAA 2296985 5.6895137 42.20563 110-119
CCCTG 4000950 5.595355 56.023987 30-34
GTAAT 2482710 5.3751073 36.562706 110-119
AGACG 2793100 4.8377028 96.25433 40-44
ACAGT 2362600 4.83678 33.644367 110-119
GTAGT 3013275 4.8242598 24.807673 70-79
GGCCC 4054570 4.7972884 56.413074 60-69
GTGAC 3142360 4.7572155 80.849525 45-49
CCCCA 2642435 4.7254486 98.31655 60-69
GAGAC 2702400 4.6806087 125.61561 40-44
ACTCC 2320040 4.387622 95.21722 30-34
ACCAG 2259430 4.3739114 93.858154 45-49
GTGTC 3287670 4.350401 24.375816 120-129
AATAC 1551745 4.295915 30.754171 110-119
GCACA 2183480 4.226884 33.36238 110-119
CGTGT 3160875 4.18262 23.259676 120-129
TGACC 2436840 4.123275 146.98135 45-49
CACTC 2122770 4.014548 81.05041 25-29
CCCTT 2417440 3.9960756 60.391468 20-24
GACCA 2054645 3.9774792 104.56437 45-49
TAGTA 1832895 3.9682477 25.8799 80-89
CTCAG 2328060 3.9392133 29.97107 140-149
CCACT 2071920 3.9183815 27.687357 240-247
TGGTG 3305925 3.9139533 26.900278 25-29
CCCAG 2412750 3.8604026 81.30979 60-69
AGTAG 2080535 3.8108578 27.482027 70-79
TGAGG 2809475 3.8054292 67.91893 35-39
CTCCC 2379480 3.7193317 57.34206 30-34
CAGGG 2810590 3.5998213 33.26731 50-59
CTGAG 2371425 3.5900977 95.578636 35-39
GACGG 2801190 3.5877817 108.36822 40-44
AGGAG 2309395 3.5787594 89.239136 35-39
CTTGG 2690075 3.5596352 35.240948 20-24
AAAAA 916140 3.5107818 18.239101 230-239
ATGGT 2192615 3.5103815 25.370394 190-199
TGTCT 2241730 3.5062094 20.925516 180-189
ACGGT 2303060 3.4866 91.14633 40-44
GCCCC 2634885 3.4844248 72.45647 60-69
TCTTG 2204490 3.4479635 19.324184 170-179
AGATA 1372670 3.400033 17.81005 150-159
AGGCT 2215705 3.3543534 41.151703 30-34
GAGGA 2164520 3.3542535 124.24345 35-39
CTTCA 1660905 3.3218005 16.522581 200-209
ATGCA 1612390 3.3009293 79.37221 25-29
TGTCC 2219925 3.2832036 18.823809 120-129
CCAGG 2264945 3.2423472 38.637093 45-49
GCCCT 2316615 3.2398012 31.891647 20-24
TTCCC 1952680 3.2278178 55.435783 50-59
CCTGG 2579160 3.2271886 31.314032 50-59
GGGTT 2720910 3.2213423 42.998226 50-59
CTCTC 1909625 3.1566472 13.706186 100-109
TCAGG 2084960 3.1564186 19.813316 140-149
GATGG 2305430 3.1227012 31.177074 15-19
GGATG 2275130 3.0816598 57.719902 20-24
CCGTG 2458780 3.0765626 23.327831 120-129
TGCAC 1797020 3.0406623 103.40444 25-29
CCAGT 1781845 3.0149858 40.856 60-69
GGTTC 2278280 3.014728 48.523014 50-59
ACAGA 1281945 3.0025575 14.161215 140-149
TCGCA 1767240 2.9902728 23.871883 100-109
TACAC 1289055 2.9495513 21.95966 110-119
CGCAC 1835425 2.936682 24.606688 100-109
GACTC 1712230 2.897193 20.503984 190-199
TCTCA 1439920 2.8798318 20.13259 130-139
TGGAG 2120580 2.872322 29.64615 25-29
TGGCC 2274885 2.8464627 38.64763 60-69
TCTCT 1621340 2.834308 15.511082 180-189
CAGAT 1376470 2.8179471 15.059049 150-159
GCACT 1662370 2.812827 73.481285 25-29
CCTGA 1657390 2.8044002 65.926315 30-34
ACCAC 1294870 2.8016617 15.45909 230-239
GTTCC 1885285 2.788281 55.349255 50-59
TTCTT 1506220 2.7845588 21.678438 170-179
AGGGT 2054370 2.7826407 50.11986 50-59
CGGAT 1830750 2.7715704 65.35852 20-24
GCCGT 2202760 2.7562163 22.36669 120-129
GGTTG 2314535 2.740226 60.01816 15-19
AGTCA 1324505 2.711563 29.932568 70-79
CGGTG 2399460 2.6862173 66.28441 40-44
TCAAA 962705 2.6651928 54.85197 70-79
TCACA 1151095 2.6338785 11.973931 140-149
AGGGA 1676790 2.5984416 9.210285 150-159
CGGCC 2179035 2.5781918 11.178909 120-129
AATAA 766740 2.5682323 12.285994 110-119
GGCTG 2293205 2.5672638 23.053728 30-34
GTTGG 2143250 2.5374382 46.57109 15-19
GATGC 1647205 2.493702 76.08637 25-29
AGATG 1349820 2.4724276 16.22138 180-189
CTGGC 1950265 2.4402802 34.54145 60-69
ACTCT 1204425 2.4088433 12.90535 190-199
TGTTC 1531250 2.3949733 12.662774 140-149
GAGAT 1298610 2.3786278 15.943325 180-189
TCCTC 1408135 2.3276744 14.705548 130-139
GTTCT 1467940 2.2959523 17.893778 160-169
GTCTC 1544080 2.2836487 12.536826 180-189
TCCAT 1133560 2.2671137 12.150919 70-79
TTGTC 1444500 2.259291 14.634488 170-179
TGTAG 1410515 2.2582376 8.624696 210-219
CTGGA 1491215 2.2575488 14.7133255 180-189
CAGAG 1303135 2.2570546 9.102196 140-149
TCATT 1063435 2.2492344 16.19887 150-159
CATTT 1058830 2.2394946 17.271776 150-159
GGCGG 2351785 2.2274697 36.902668 20-24
TTCAT 1043910 2.207938 16.392738 140-149
CACTA 964685 2.2073443 14.239511 240-247
ACAGC 1136840 2.2007487 11.501042 120-129
TTCAC 1097025 2.1940439 16.736032 200-209
GCGGA 1704840 2.1835697 78.17476 20-24
TAGTC 1215515 2.175057 25.503523 70-79
TTGGT 1545685 2.163003 39.243465 25-29
TCTGC 1444260 2.1360178 10.909098 210-219
ATATA 725470 2.1239772 10.092296 110-119
ACTAC 920540 2.1063337 12.144556 230-239
CTCTG 1423000 2.104575 14.893589 180-189
GTGAA 1137005 2.0826204 16.52998 190-199
TTGGA 1300280 2.081751 16.497778 170-179
GATAC 1011570 2.070914 12.828322 150-159
TCTCG 1397265 2.0665138 16.306341 100-109
AATAT 703860 2.0607092 12.03461 110-119
CTACT 1025235 2.0504642 8.199771 230-239
GATAT 947015 2.050303 9.435388 180-189
ATTTG 1079130 2.04211 15.081572 150-159
TTGGG 1724395 2.041547 73.34858 15-19
GAGTC 1348085 2.0408647 12.48696 210-219
GTTCA 1137420 2.035313 15.169294 140-149
GTCAA 991400 2.0296214 39.924076 70-79
CTCGC 1447450 2.024268 16.42346 100-109
ATATG 926895 2.0067427 9.621511 180-189
ATGTA 925920 2.004632 10.483509 70-79
TGAAT 924350 2.0012326 14.046673 190-199
GAATC 973470 1.9929147 14.2393875 190-199
CCTTC 1196245 1.9774162 13.558103 200-209
TCGGC 1576190 1.9722166 8.124975 190-199
CAGCC 1230020 1.9680333 10.57233 120-129
CCTTG 1320540 1.9530396 39.897667 20-24
AATAG 781475 1.9356732 8.4706135 240-247
CACGG 1348080 1.9298233 7.084144 200-209
GAACT 941135 1.9267175 11.5576 160-169
TCCCA 1013965 1.9175941 11.872766 240-247
ACACA 732495 1.9175427 12.693691 110-119
GTCAC 1129795 1.9116788 9.360146 140-149
ATAGA 769385 1.9057271 10.090328 240-247
AATCG 929745 1.9033996 12.879607 190-199
TACAG 927355 1.8985068 11.6337185 160-169
TACCA 826255 1.8905957 12.065434 230-239
GCTGT 1419235 1.8779993 9.609515 140-149
TCAGC 1106950 1.873024 11.861271 140-149
TTGCA 1042475 1.8654172 13.24902 150-159
GTCCT 1254920 1.8559897 12.599853 130-139
GTTGT 1324785 1.8538797 7.0600905 210-219
TACTA 763395 1.8472637 10.573603 230-239
TGGAA 1006145 1.8429277 8.778301 170-179
GGTCA 1216300 1.8413552 9.493747 130-139
TTTGC 1176140 1.8395585 11.600609 150-159
GTCTG 1385355 1.8331677 8.68414 210-219
CACCA 843000 1.8239675 11.147411 230-239
GCTGA 1204510 1.8235064 35.592632 30-34
ATTGT 959610 1.8159344 8.411298 170-179
CAGGC 1265275 1.811285 13.912719 140-149
AGTAT 835575 1.8090335 11.674177 220-229
TGGGG 1798650 1.8015897 43.965866 15-19
CATAG 870275 1.781651 7.878432 210-219
AGTCT 990695 1.7727616 12.5816145 210-219
CTGTT 1131135 1.7691677 12.289333 140-149
ATCTC 883955 1.767905 7.082973 100-109
TTCAG 976980 1.7482197 9.87676 150-159
CTCCA 924125 1.7476901 8.635403 240-247
CAGCT 1024925 1.7342329 9.407124 150-159
TTGTA 909105 1.7203604 9.555426 220-229
ATAGT 774465 1.6767294 7.0445085 220-229
AGAAC 710895 1.6650504 10.620944 160-169
CCCAC 929600 1.6623974 10.639919 240-247
GGAGG 1448490 1.6598946 25.664236 30-34
GTGGA 1224785 1.6589693 36.430786 25-29
GGGGC 1751045 1.6584847 40.55062 15-19
ACGGC 1158390 1.6582755 12.540536 120-129
TCTGG 1249965 1.6540132 12.063128 180-189
CCAGA 852230 1.6497872 21.88355 60-69
GCAGA 950535 1.6463448 12.916707 150-159
TGACT 918815 1.6441386 12.046964 190-199
GACGT 1079090 1.6336331 9.964353 170-179
CAAAG 694250 1.6260647 28.399675 70-79
AACCA 614145 1.6077232 12.302129 70-79
TGCAG 1057750 1.6013266 12.450304 150-159
GGGCG 1685850 1.5967361 48.02827 20-24
CACTG 939890 1.5903487 6.7533746 230-239
GAGAA 757670 1.5877556 10.635927 160-169
GGACG 1235555 1.5825064 7.8527546 170-179
TGGTT 1129930 1.5812031 16.813148 10-14
CTCTT 900790 1.5746952 9.139508 200-209
AACTG 767090 1.5704079 10.25676 160-169
CAAAA 489685 1.5509863 19.808443 70-79
AGCTC 915395 1.5489018 9.66717 140-149
ACACG 799465 1.5476424 9.698655 120-129
ATAAA 459695 1.5397704 6.2875695 110-119
ATAAT 524470 1.5355043 5.2531424 110-119
CGACT 904100 1.5297899 9.309333 190-199
GTCCC 1091680 1.5267214 16.063778 50-59
ACTGA 743585 1.5222877 7.5262027 180-189
CTGGT 1144690 1.5147082 7.0205507 160-169
CCACC 841145 1.5042139 8.322997 230-239
TCGAC 888950 1.504155 9.88308 190-199
TCAGA 734720 1.5041391 8.856804 130-139
ATGGG 1100195 1.4902123 50.33554 15-19
ACGGA 852790 1.4770486 9.189439 200-209
GCTCT 995855 1.4728403 8.979332 130-139
GAGGG 1277540 1.4639949 9.003455 200-209
ATCGG 966495 1.4631757 9.001993 190-199
TGGAC 963615 1.4588157 10.880251 170-179
CCTCG 1038660 1.4525728 9.040612 130-139
TCCAC 764825 1.4464245 10.361958 240-247
GGCCG 1352800 1.4320763 11.83333 120-129
CTTGA 796805 1.4258124 9.2815275 200-209
AATTG 656200 1.4206836 9.381922 170-179
CGTAG 933610 1.4133914 7.994459 210-219
TAGTT 746800 1.4132198 8.90919 220-229
GGGAG 1212780 1.3897833 6.6430235 150-159
CTCGA 817870 1.3838836 8.85347 190-199
CCATC 728360 1.3774626 11.434851 240-247
AGCCG 950815 1.3611246 10.102059 120-129
CATGT 757665 1.3557749 9.973174 70-79
CAGAC 698990 1.3531379 11.379664 60-69
TATGG 837500 1.3408394 6.9344373 180-189
ACTAT 551715 1.3350403 6.9659905 230-239
GAATT 616340 1.3343862 10.393777 170-179
GGCTC 1051210 1.3153324 8.0497675 130-139
GTGCT 987530 1.3067465 7.265944 220-229
TATAC 539540 1.3055792 6.4022574 110-119
CAATA 469410 1.2995343 7.2112694 100-109
ACCAT 567885 1.2994062 9.839034 45-49
GAGGC 1013805 1.2984878 22.29647 30-34
ACGTG 851070 1.2884339 9.425698 170-179
CCATG 760800 1.287318 10.005327 70-79
ACTGT 718685 1.2860236 5.4591002 230-239
ACTCG 755805 1.2788662 9.158006 190-199
TGATA 590150 1.2776843 7.264737 180-189
GGAAT 696170 1.2751551 8.260772 170-179
GCTCA 753160 1.2743907 9.218443 140-149
TTCCA 636770 1.2735363 7.3234286 230-239
CCTCA 669885 1.2668755 5.66743 130-139
ACTTC 633195 1.2663864 5.3919864 210-219
AAAGT 507940 1.2581412 21.106634 70-79
TGTGC 950490 1.2577335 6.478374 220-229
GTGTT 892915 1.2495288 7.1491184 160-169
AAGTA 503665 1.2475524 16.791906 70-79
GGTGG 1233270 1.2352856 36.521515 25-29
ATCCA 536410 1.2273867 16.393822 240-247
TATCA 506885 1.2265606 5.5133767 70-79
GTCCA 721600 1.2209891 11.361028 70-79
CTGAT 681175 1.2189027 6.2444267 180-189
CTGAA 594905 1.2179059 12.809013 35-39
GAACC 626065 1.2119663 9.195269 70-79
GGAGT 894295 1.211321 6.7543426 210-219
GCGTA 792840 1.2002796 8.941803 210-219
TTGAG 748735 1.1987264 8.375061 200-209
GGGCC 1127265 1.1933247 28.343166 15-19
AGAGA 566310 1.186746 11.896983 35-39
AGTTC 659310 1.1797771 5.678248 160-169
AGATC 575900 1.1789984 5.8953485 130-139
TGGGC 1048405 1.173699 30.354034 15-19
GAAGA 552600 1.1580156 16.843107 35-39
ACTGG 764235 1.1569746 6.952106 160-169
TGCCC 825460 1.1544111 9.428149 60-69
CTGCG 916290 1.146513 6.268106 210-219
ATCAT 467360 1.130918 5.039739 90-99
AGAGC 651950 1.1291898 6.605328 140-149
GCATA 551375 1.1287901 7.712796 210-219
CCCAA 520680 1.1265758 9.797079 240-247
GTCTA 622820 1.1144816 7.5175366 180-189
ACAAT 399655 1.1064215 8.437592 100-109
TCACG 647860 1.0962169 8.203246 200-209
AGGTT 683860 1.0948615 18.596668 10-14
GCGGT 970640 1.0866404 6.758895 130-139
AAGAG 517625 1.0847228 11.785206 35-39
CGATG 715220 1.0827708 21.116245 15-19
TAGAT 499970 1.0824432 7.847714 240-247
TATGT 569945 1.0785452 7.108608 220-229
TCTAC 534065 1.068127 7.1240497 180-189
AAAAG 376840 1.0678979 12.778697 70-79
CGGCT 842640 1.054358 7.2793627 130-139
CTCGG 833395 1.0427902 7.3577166 130-139
TCTGT 666140 1.0418856 5.368172 210-219
ATATC 430450 1.0416032 5.4864864 70-79
TGGTC 783530 1.0368041 8.158676 50-59
TTGGC 779700 1.031736 5.0323706 170-179
CCAAT 450300 1.0303541 10.906137 240-247
ATAAC 368865 1.0211812 6.790894 80-89
GTGGT 853375 1.0103285 7.1153054 50-59
TGTAA 459185 0.994143 6.5576606 230-239
TACTG 555340 0.99373204 5.253125 180-189
TATAA 338510 0.9910645 6.8742447 240-247
TCCAG 580345 0.9819776 5.524737 240-247
AGCTT 546070 0.97714424 5.8414507 150-159
CGGTC 779150 0.97491586 6.869201 130-139
GCTTC 657205 0.9719869 5.471767 150-159
CATCA 423855 0.969844 5.7109966 230-239
GTCAG 630815 0.95499015 5.196664 130-139
ACCCA 437545 0.9466997 11.373255 240-247
ACTAA 340490 0.9426268 11.126067 240-247
CGGAG 733075 0.93892705 6.346685 210-219
GAGTT 583110 0.93356055 5.0447803 160-169
ACCGT 543895 0.9203021 12.766673 45-49
TGAAC 440895 0.9026123 5.4100413 190-199
CTACC 475530 0.8993146 6.684897 230-239
CTTCC 539495 0.8917957 5.0052295 230-239
TGAAG 478440 0.8763451 8.985259 35-39
ATCAA 313280 0.8672975 12.220661 70-79
GGAAG 558825 0.8659844 9.29897 30-34
CCAGC 540230 0.8643686 5.5220304 80-89
GAGCT 570245 0.8632933 5.265986 140-149
AACTT 355610 0.8605053 5.143848 200-209
TGCAT 479525 0.85806775 6.20151 210-219
GTATG 528355 0.84589756 5.0918703 220-229
TGCGT 636755 0.84258443 6.7933407 210-219
AGCTG 556430 0.84237874 6.435363 30-34
ACGTC 489370 0.82804257 7.345736 70-79
CCTTT 461410 0.8066032 6.127781 50-59
AATCC 349030 0.79863304 10.297532 240-247
AAAGG 380155 0.79664385 7.3053374 10-14
AGCCA 402515 0.7792076 6.980869 80-89
TAACC 339550 0.77694136 6.5592337 80-89
CTAAT 317140 0.7674156 8.900498 240-247
TGAGA 411380 0.7535133 5.6913295 240-247
CCCAT 387405 0.73265404 5.0115056 240-247
TGGAT 457550 0.7325386 7.10056 10-14
ATACC 319640 0.73138434 6.6480246 240-247
CAATC 319280 0.7305606 10.275986 240-247
TCGAA 354890 0.72654057 12.701782 70-79
AAATG 292715 0.72503996 5.033976 9
GACCG 506020 0.7243852 12.904013 45-49
TAGGT 452085 0.7237891 6.091616 10-14
AGGAT 393465 0.7206989 7.8567314 10-14
GGTCC 558715 0.6990953 8.932211 50-59
GTCGA 454350 0.68784 8.873529 70-79
CGAAC 348295 0.6742459 9.073604 70-79
AATGG 366035 0.67045605 5.4743824 10-14
ACGAT 317470 0.6499333 7.9871173 10-14
CTTTG 409120 0.6398899 5.3228703 50-59
AAGGT 345950 0.633667 6.9082246 10-14
AAAGC 263890 0.6180802 8.595788 70-79
CATCC 325795 0.61613816 7.554919 240-247
CGTCC 423685 0.59252626 5.8834662 70-79
ATAGG 319015 0.58433086 5.117505 10-14
GAAGC 326740 0.56591994 8.420281 30-34
TTGCC 379230 0.56087 6.342656 60-69
TCAAG 264730 0.54196256 5.6510787 70-79
AAGCT 253685 0.51935095 13.246817 30-34
GACGA 293980 0.509179 6.1354666 40-44
CGGTT 376915 0.49875182 10.052923 10-14
AAGCA 204520 0.4790245 5.455114 70-79
GGGAT 353330 0.47858492 5.674154 10-14
TCGAT 234960 0.42044023 6.719674 10-14
Produced by FastQC (version 0.10.1)