FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_6_SL254633.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_6_SL254633.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18129601
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 21468820 8.407844 58.48947 110-119
CACAG 27475520 7.277982 42.086506 110-119
TCCCT 29607820 6.575741 96.302345 30-34
CAGTA 23777175 6.574594 43.465816 60-69
ATACA 17212915 6.4578404 31.612566 110-119
GGAGA 29613570 6.219176 94.00957 35-39
AGTAA 17764505 5.934368 43.812687 110-119
GGTGA 32051055 5.815878 94.09191 45-49
CCCTG 30014235 5.6860466 58.39864 30-34
GTAAT 19169750 5.533102 37.45314 110-119
GGCCC 31677145 5.118871 60.70002 60-69
ACAGT 18275730 5.0533967 34.7482 110-119
AGACG 21418145 5.051675 102.67338 40-44
CCCCA 19832695 4.8836613 105.00421 60-69
GTGAC 23813400 4.8529496 84.948814 45-49
GTAGT 22781915 4.8463883 24.772383 70-79
GAGAC 20225265 4.7703233 133.23064 40-44
ACCAG 17282900 4.578062 101.76527 45-49
AATAC 11797645 4.4261713 32.027054 110-119
GTGTC 25017070 4.405059 25.317856 120-129
CGTGT 24458195 4.3066506 24.176619 120-129
TGACC 18761120 4.293927 155.58626 45-49
GACCA 16040405 4.2489376 113.1892 45-49
GCACA 15980670 4.2331147 35.662025 110-119
ACTCC 16126925 4.1453285 95.64314 30-34
CCCTT 18504460 4.1097426 61.895897 20-24
TAGTA 14165120 4.0885797 25.149616 80-89
CCCAG 18146985 3.9788427 86.426346 60-69
CTCAG 17207935 3.9384444 30.798409 140-149
TGGTG 25056605 3.9284933 27.18642 25-29
AGTAG 15750670 3.877896 28.614216 70-79
CCACT 15051555 3.868911 27.32077 240-247
CACTC 14938960 3.8399692 81.50067 25-29
TGAGG 21109740 3.8305032 71.11383 35-39
CTCCC 17709435 3.7679002 60.375393 30-34
CAGGG 21646160 3.762792 36.057915 50-59
GACGG 21591940 3.7533665 114.07544 40-44
AGGAG 17439170 3.662418 93.648506 35-39
CTTGG 20798545 3.6622524 35.644985 20-24
CTGAG 17965060 3.6611128 99.87392 35-39
GCCCC 20065030 3.6414924 77.141464 60-69
ACGGT 17738275 3.614896 94.868675 40-44
TCTTG 17348095 3.5811472 20.518946 170-179
TGTCT 17295630 3.5703166 22.206865 180-189
ATGGT 16733300 3.5596685 26.032433 190-199
AGATA 10613185 3.5454152 19.849094 150-159
CCAGG 17677220 3.451078 41.813393 50-59
GAGGA 16394245 3.442972 130.99272 35-39
AGGCT 16679430 3.3991134 43.409267 30-34
GCCCT 17751200 3.3628764 32.933914 20-24
TGTCC 16989445 3.3597422 19.891562 120-129
CTTCA 12339775 3.3109927 18.071295 200-209
TTCCC 14865790 3.3016136 58.489944 50-59
CTCTC 14805005 3.2881136 13.988433 100-109
ATGCA 11854205 3.2777896 80.06318 25-29
GGGTT 20754745 3.2540276 44.840355 50-59
TCAGG 15805310 3.2209759 20.345152 140-149
CCTGG 18975220 3.2007995 33.29153 50-59
CCGTG 18820495 3.1747005 24.57284 120-129
GATGG 17463415 3.1688528 31.693806 15-19
TCGCA 13726235 3.1415744 25.149696 100-109
ACAGA 9814105 3.140715 14.50177 140-149
CGCAC 14104775 3.0925627 25.637089 100-109
GGTTC 17430635 3.0692236 50.883614 50-59
TGCAC 13235905 3.0293505 103.78011 25-29
TCTCT 13034180 3.021799 17.032347 180-189
TACAC 9652130 2.9973903 22.653072 110-119
GGATG 16456825 2.9862006 57.12563 20-24
TGGCC 17687235 2.9835386 41.0987 60-69
TCTCA 11103085 2.9791656 20.922934 130-139
TGGAG 16337530 2.9645534 30.5734 25-29
CCAGT 12913295 2.9555135 42.944565 60-69
GACTC 12813555 2.9326859 20.04878 190-199
TTCTT 11963170 2.895154 22.949934 170-179
GTTCC 14631030 2.8933547 58.276573 50-59
CCTGA 12559685 2.8745813 68.627914 30-34
AGGGT 15735305 2.855276 53.088722 50-59
AATAA 6274585 2.8440068 13.041068 110-119
ACCAC 9537890 2.8374543 16.331043 230-239
TCAAA 7508950 2.8171637 58.276134 70-79
GCCGT 16650925 2.8087306 23.331285 120-129
TCACA 8983205 2.7896612 12.422857 140-149
GCACT 12163775 2.783968 73.519745 25-29
CGGTG 18494445 2.777803 68.80611 40-44
CAGAT 10030750 2.773589 16.332685 150-159
CGGCC 17001075 2.74729 11.699548 200-209
CGGAT 13476645 2.7464151 65.00836 20-24
GGTTG 17199375 2.6965997 58.665234 15-19
AGTCA 9541925 2.6384244 31.29585 70-79
AGGGA 12448695 2.614363 9.407638 150-159
AGATG 10562750 2.6006036 17.747944 180-189
GGCTG 17279595 2.595337 24.35228 30-34
TGTTC 12521785 2.5848575 13.671788 140-149
CTGGC 15117185 2.5500145 36.965416 60-69
GAGAT 10172690 2.5045688 17.607388 180-189
GTTGG 15898410 2.4926279 45.909584 15-19
GATGC 12180175 2.4822066 75.76581 25-29
TCATT 8729805 2.445114 18.162092 150-159
ACAGC 9159080 2.426146 13.044313 120-129
CATTT 8633020 2.4180057 19.193478 150-159
GTCTC 12132800 2.399318 13.894545 180-189
GTTCT 11603935 2.3953867 18.791233 160-169
TTCAT 8543215 2.3928523 18.210121 140-149
TTGTC 11524670 2.379024 15.404106 170-179
ACTCT 8702730 2.3351054 13.137589 200-209
AATAT 5941145 2.3267334 13.047981 110-119
TCCTC 10474985 2.326439 16.129528 130-139
CTGGA 11366355 2.3163579 16.158806 180-189
CAGAG 9783675 2.3075738 9.24607 140-149
CACTA 7412020 2.3017426 15.955656 240-247
TTCAC 8543545 2.2923932 18.831915 200-209
ATATA 5852945 2.2921915 10.990235 110-119
GTGAA 9138870 2.250037 18.456991 190-199
ACTAC 7168930 2.226253 12.875998 230-239
TAGTC 9311100 2.2245414 26.251917 70-79
GGCGG 17338805 2.2213871 37.17145 20-24
ATTTG 8899410 2.2194443 16.490202 150-159
GAATC 8020070 2.2176182 16.6608 190-199
TCCAT 8185765 2.1963942 11.386811 70-79
TGAAT 7565175 2.1835907 16.001717 190-199
TTGGA 10230980 2.1764326 17.427378 170-179
GCGGA 12432995 2.1612504 78.54204 20-24
GTCAA 7798720 2.156413 42.63909 70-79
TGTAG 10125120 2.153913 8.728351 210-219
GAGTC 10551190 2.1502347 13.799253 210-219
TTGGT 11670440 2.1450927 39.103085 25-29
GATAC 7740195 2.1402302 14.193238 150-159
CTCTG 10806195 2.1369755 16.010881 180-189
TCGGC 12666435 2.136614 9.118769 190-199
TCTGC 10722080 2.1203418 11.431483 210-219
GTTCA 8831095 2.109862 16.604641 140-149
AATCG 7585540 2.097467 14.873701 190-199
CTCGC 11030425 2.0896595 17.19915 100-109
CTACT 7786285 2.089206 8.863011 230-239
CAGCC 9407535 2.0626626 11.854451 120-129
CCTTC 9272290 2.0593264 14.870038 200-209
CACGG 10528325 2.0554178 8.227487 200-209
TACAG 7426645 2.0535312 12.989773 160-169
ATATG 7019850 2.0261896 9.413334 180-189
TCTCG 10242815 2.0255647 16.733826 100-109
AATAG 6062440 2.0252042 8.708418 240-247
ACACA 5593065 2.0101995 13.836278 110-119
TTGCA 8355505 1.9962374 14.621531 150-159
TTGGG 12644700 1.9824958 71.948074 15-19
GATAT 6789655 1.9597465 9.242596 180-189
ATAGA 5818710 1.9437844 9.565936 240-247
CCTTG 9827210 1.9433765 40.653324 20-24
GTCCT 9795630 1.9371314 13.989377 130-139
TGGAA 7850955 1.9329456 9.813602 170-179
TACTA 5946235 1.9275503 10.904604 240-247
TACCA 6197330 1.9245301 12.774896 230-239
GCTGT 10929285 1.9244516 10.514918 140-149
TTTGC 9294035 1.9185568 12.710931 150-159
AGTAT 6639870 1.916513 12.499253 220-229
ATTGT 7632055 1.9033759 9.006401 170-179
GTTGT 10335420 1.8997085 7.549584 210-219
AGTCT 7933270 1.8953599 14.204132 210-219
TCCCA 7354315 1.8903823 11.789989 240-247
CAGGC 9619030 1.8778986 14.316837 140-149
GCTGA 9151075 1.864904 37.692913 30-34
CTGTT 8980670 1.8538693 13.356797 140-149
GGTCA 9095030 1.8534828 9.619442 130-139
CATAG 6628395 1.8328084 7.9773283 210-219
GTCAC 7897515 1.8075335 9.59602 140-149
AAAAA 3396235 1.7816094 6.654208 4
TCTGG 10049410 1.7695217 13.175065 180-189
GTCTG 10038500 1.7676004 9.567659 210-219
GAACT 6354490 1.7570711 10.740617 160-169
GCAGA 7430595 1.7525771 14.729964 150-159
TGGGG 13089915 1.7505944 43.373688 15-19
ATAGT 6047350 1.7454898 7.4358053 220-229
TTGTA 6914680 1.724468 9.763579 210-219
TCAGC 7499525 1.7164443 10.98106 140-149
AGAAC 5362900 1.7162378 11.053811 160-169
AACCA 4768410 1.7138107 13.090783 70-79
ACGGC 8754590 1.7091361 12.491749 120-129
CAGCT 7407540 1.6953913 9.3428755 150-159
ATAAT 4308215 1.6872281 5.8196483 100-109
TGCAG 8248450 1.6809574 13.437276 150-159
CACCA 5629870 1.6748463 11.27428 230-239
CAAAG 5204575 1.665571 31.161991 70-79
ATAAA 3646485 1.652799 7.0830197 8
GGGGC 12797470 1.6395674 40.68434 15-19
GTGGA 9021275 1.6369703 37.298843 25-29
ATGTA 5646950 1.6299194 9.6580925 70-79
TTCAG 6795715 1.6235836 10.135601 150-159
ATCTC 6047425 1.6226373 6.993051 100-109
ACGGA 6878270 1.6223061 10.689161 200-209
GGAGG 10464950 1.6197762 26.256844 30-34
CCCAC 6551870 1.6133518 10.761951 240-247
GAGAA 5637520 1.6064026 11.003653 160-169
GGACG 9231485 1.6047262 7.9574437 170-179
CAAAA 3680065 1.5979292 20.266603 70-79
AACTG 5774660 1.5967431 10.371769 160-169
GACGT 7834195 1.5965362 9.887241 170-179
TGACT 6670345 1.593631 11.532735 190-199
GGGCG 12339595 1.580906 47.902023 20-24
CGACT 6868345 1.5719835 9.5809 200-209
CTCTT 6731485 1.5606041 9.338025 200-209
TCGAC 6801835 1.5567614 10.008459 190-199
CCTCG 8183605 1.550343 9.868918 130-139
TGGTT 8394745 1.5430017 15.735425 10-14
AAATA 3404205 1.5429837 6.4892707 6
AGCTC 6724255 1.5390052 9.547573 140-149
CACTG 6714560 1.5367863 6.4120636 230-239
GCTCT 7701850 1.5230767 9.863239 130-139
AATTG 5253285 1.5162932 10.039824 170-179
GTCCC 7999240 1.515416 16.066978 50-59
AGCCG 7701980 1.5036376 11.398228 120-129
CCAGA 5671390 1.5022926 22.439426 60-69
ACTGA 5433070 1.5022907 7.486194 180-189
CAATA 3994690 1.4987043 8.139975 100-109
ATGGG 8214390 1.4905556 51.309013 15-19
CTCCA 5703750 1.4661145 7.9218264 240-247
ATCGG 7165500 1.4602623 10.187731 190-199
GGCCG 10112170 1.4549934 12.029969 120-129
CCACC 5875400 1.4467758 8.485957 230-239
GAATT 4996910 1.4422938 11.295384 170-179
CTCGA 6280735 1.4374954 8.999157 190-199
CTGGT 8162710 1.4373075 6.854733 160-169
TCAGA 5192790 1.4358512 8.000398 140-149
TGGAC 7013720 1.429331 10.820017 170-179
CGTAG 6988555 1.4242026 8.652031 210-219
TCCAC 5523055 1.419668 10.100054 240-247
GAGGG 9151740 1.4165162 9.265971 200-209
ACTAT 4367910 1.4159158 7.238495 230-239
CCATC 5411080 1.3908852 10.965018 240-247
ACACG 5192905 1.375547 9.17162 120-129
GGGAG 8850050 1.3698204 6.707814 150-159
CTTGA 5717580 1.3660035 9.551158 200-209
TATAC 4160580 1.3487067 6.9203334 120-129
TAGTT 5392980 1.3449678 8.655885 220-229
GGCTC 7970950 1.3445649 8.839336 130-139
AAAGT 3991160 1.3332773 23.14538 70-79
AAGTA 3987390 1.332018 18.496647 70-79
TATGG 6225435 1.3243345 6.714306 190-199
ACCAT 4217120 1.3095924 9.940133 45-49
ACTCG 5699690 1.3045093 9.431056 190-199
GGAGT 7176760 1.302271 7.7119203 210-219
GAACC 4894255 1.2964377 9.8900585 70-79
CAGAC 4874710 1.2912605 10.980812 60-69
TTCCA 4807095 1.2898337 7.9599457 230-239
ACGTG 6290805 1.2820078 9.457393 170-179
TATCA 3935035 1.2755934 5.727999 90-99
GTGCT 7242715 1.2753127 7.4144607 220-229
TCACG 5538775 1.26768 9.358122 200-209
GAGGC 7291590 1.2675107 23.363947 30-34
ACTTC 4697535 1.2604367 5.5855675 220-229
GTGTT 6829945 1.2553825 7.798193 160-169
ATCAT 3859170 1.2510009 5.890362 90-99
GGAAT 5079915 1.2507013 8.959942 170-179
GCGTA 6119445 1.247086 9.804072 210-219
ACTGT 5204475 1.243416 5.1238084 230-239
CATGT 5196900 1.2416061 9.10785 70-79
GCTCA 5417595 1.2399449 9.303985 140-149
TGATA 4293455 1.2392507 6.851428 180-189
AGAGT 5031600 1.2388059 5.13026 210-219
GGTGG 9246760 1.2366258 37.27095 25-29
GGGCC 8594410 1.2366099 29.340021 15-19
CCTCA 4798865 1.2335192 6.0939956 130-139
TTGAG 5745895 1.2223221 8.684941 200-209
CTGCG 7195595 1.2137756 7.059528 210-219
GTCCA 5266035 1.2052568 10.802566 70-79
CCATG 5235895 1.1983587 9.272903 70-79
TGGGC 7923010 1.1900094 31.042978 15-19
AGAGA 4152695 1.183304 12.282953 35-39
AGTTC 4950660 1.1827762 6.080685 160-169
ACAAT 3146805 1.1805998 9.086216 100-109
TGTGC 6639295 1.1690611 6.2015405 220-229
CTGAT 4891325 1.1686004 6.109426 180-189
ACTGG 5618195 1.1449361 6.9443984 160-169
GTCTA 4772035 1.1401005 7.771458 180-189
CAGTG 5577885 1.1367213 5.0279827 160-169
TATGT 4484605 1.1184261 7.6639442 220-229
GCATA 4040475 1.1172261 7.5151367 210-219
AGTGA 4530090 1.1153315 5.118882 160-169
CGGCT 6590600 1.1117232 7.9610863 130-139
CTCGG 6585785 1.1109111 8.13435 130-139
ATCCA 3543120 1.100287 15.135445 240-247
TATAA 2804905 1.0984861 7.7142267 240-247
ATAAC 2915355 1.0937657 7.897655 80-89
GCGGT 7263790 1.0909967 7.042802 130-139
CCAAT 3505500 1.0886045 10.858847 240-247
TCTAC 4045520 1.0854889 7.3752565 180-189
TGCCC 5684560 1.0769114 8.896171 60-69
AAAAG 2769075 1.070595 13.174431 70-79
AGGTT 5024170 1.0687898 18.295313 10-14
CCCAA 3570625 1.0622354 9.727809 240-247
TTGGC 5924600 1.0432161 5.4152718 170-179
CGGAG 5967360 1.0373172 7.404655 210-219
CTGAA 3743960 1.0352372 13.227641 35-39
TAGAT 3568265 1.0299337 7.8041368 240-247
TCATA 3164545 1.0258291 5.0890427 90-99
ATATC 3153750 1.0223297 5.425357 70-79
CATCA 3267660 1.0147452 6.5244837 240-247
GAAGA 3520620 1.0031952 17.57669 35-39
CGGTC 5921685 0.9988884 7.0993786 130-139
AGAGC 4221470 0.9956742 6.3185296 140-149
TGGTC 5607895 0.98745006 7.569647 50-59
GTGGT 6285080 0.9854046 6.5836263 50-59
ATACG 3557075 0.9835618 5.3351545 120-129
AAAAT 2169735 0.98345006 5.2418933 5
GAGTT 4582600 0.9748548 5.6380625 160-169
TGTAA 3357245 0.96902555 6.5219197 230-239
CAGCG 4936405 0.9637217 5.244925 160-169
GCTTC 4872340 0.96352804 5.5747323 150-159
GCCAC 4379530 0.9602401 5.1215334 80-89
TACTG 4016850 0.959677 5.076103 180-189
AGCTT 4002725 0.95630234 6.0026107 150-159
CTACC 3709775 0.9535752 7.267655 230-239
CGATG 4676490 0.9530252 21.874046 15-19
TAGAC 3366585 0.9308896 5.199247 110-119
ACTAA 2474990 0.9285522 12.34079 240-247
AAGAG 3207420 0.9139494 12.277043 35-39
ACCGT 3984730 0.912 11.982351 45-49
CTATA 2754975 0.8930615 5.0031686 230-239
TGAAG 3597510 0.88572556 9.327844 35-39
TGCGT 5007465 0.881725 7.2515984 210-219
ATCAA 2332710 0.87517244 12.6554365 70-79
GAGCT 4281380 0.8725056 5.4235115 140-149
CTTCC 3878840 0.8614699 5.749638 230-239
CACAA 2338205 0.8403726 5.0005994 100-109
AGCTG 4121685 0.8399611 6.612951 30-34
TGCAT 3463890 0.82756776 5.991729 210-219
ACCCA 2780405 0.8271507 11.282859 240-247
TAACC 2645130 0.8214236 7.308941 80-89
TACAA 2188835 0.82119423 5.5479565 100-109
CCAGC 3681415 0.80717385 5.7234483 80-89
AGCCA 3046700 0.8070394 7.8761077 80-89
AAAGA 2082495 0.8051457 5.470255 70-79
CCTTT 3401265 0.7885374 5.615548 50-59
TCGAA 2821420 0.78014696 13.730744 70-79
AAATG 2318400 0.77447915 5.231421 9
TGAAC 2767650 0.765279 5.4194098 190-199
CGGCG 5295180 0.7618989 5.215492 130-139
AATCC 2435475 0.75631696 9.640708 240-247
GTATG 3550710 0.75534123 5.021187 220-229
AAAGG 2636080 0.7511469 7.8461404 10-14
CGTTG 4265605 0.7510968 5.123734 170-179
CTAAT 2306175 0.74757713 9.992873 240-247
GCGGC 5133930 0.73869735 5.276698 130-139
AGCGT 3600115 0.73367 5.244447 160-169
TGAGA 2979910 0.7336692 6.115737 240-247
TAGGT 3438135 0.73139316 6.0315495 10-14
AGGAT 2924530 0.7200344 8.575283 10-14
ATACC 2305225 0.71586883 6.295486 240-247
GTCGA 3494020 0.7120488 9.401925 70-79
CGAAC 2679550 0.70978516 9.870957 70-79
GGAAG 3375500 0.70889217 9.692924 30-34
GAAGT 2816315 0.69339126 5.0623765 70-79
CAATC 2231680 0.6930301 9.875698 240-247
GCGTT 3881165 0.68340373 5.162318 170-179
GGTCC 4002980 0.6752353 8.708266 50-59
AATGG 2701550 0.66513556 5.5130296 10-14
ACGTC 2884835 0.66026294 7.040497 70-79
ACGAT 2384585 0.6593583 7.9988346 10-14
TTTCG 3181840 0.6568235 5.140833 100-109
AAAGC 2045490 0.6545988 8.997989 70-79
TGGAT 3025740 0.64366454 6.444481 10-14
GACCG 3193795 0.6235163 12.529354 45-49
CTATC 2313420 0.6207339 5.2089396 90-99
GTAGC 3036135 0.6187362 5.002862 80-89
AAGGT 2469600 0.6080283 6.7223673 10-14
AAGTC 2167975 0.5994637 5.0284057 70-79
ATAGG 2421660 0.5962252 5.40147 10-14
TATGA 2045900 0.5905227 5.017687 240-247
GAAGC 2387965 0.56322443 8.782714 30-34
CGTCC 2965635 0.56182474 5.5920806 70-79
CATCC 2167020 0.55701935 6.7615676 240-247
AAGCT 1953805 0.5402439 14.1093025 30-34
TCAAG 1936940 0.53558064 6.0059204 70-79
TTGCC 2650770 0.52420217 5.7705407 60-69
GACGA 2187215 0.51587564 7.0314426 40-44
CGGTT 2827445 0.4978625 9.553724 10-14
GGGAT 2675770 0.4855363 5.9916697 10-14
TAAAG 1427730 0.4769441 5.624891 9
AAGCA 1483460 0.47473764 5.6754575 70-79
TCGAT 1733310 0.41411003 6.2472234 10-14
GCGAT 1621900 0.33052814 5.1504397 10-14