FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_3_SL254630.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_3_SL254630.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2898577
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3158380 7.747485 54.138268 110-119
CACAG 4199095 6.748879 37.410477 110-119
TCCCT 4534300 6.3343806 89.31659 30-34
CAGTA 3599820 6.1664777 40.662903 60-69
ATACA 2586785 5.953553 29.545036 110-119
GGAGA 4542755 5.88678 89.83081 35-39
GGTGA 4929030 5.6428633 88.199196 45-49
CCCTG 4654760 5.4783783 54.109093 30-34
AGTAA 2643085 5.462209 39.328815 110-119
GTAAT 2862800 5.2267027 34.661877 110-119
GGCCC 4816385 4.775697 55.734 60-69
AGACG 3274430 4.725556 92.61069 40-44
ACAGT 2751960 4.7140965 30.905127 110-119
GTGAC 3689160 4.7035346 78.16584 45-49
GAGAC 3248845 4.688633 120.96178 40-44
CCCCA 3138965 4.6571517 95.11928 60-69
GTAGT 3399825 4.619913 23.41864 70-79
AAAAA 1410675 4.433678 24.06018 230-239
ACTCC 2782005 4.3991847 90.646 30-34
GTGTC 3841520 4.326926 24.34976 120-129
ACCAG 2673765 4.2973347 93.91956 45-49
GCACA 2589800 4.1623845 31.11149 110-119
CGTGT 3682160 4.1474295 23.406101 120-129
AATAC 1796155 4.1338987 29.465742 110-119
CCCTT 2899635 4.0507665 64.070885 20-24
TGACC 2833660 4.0234933 144.69072 45-49
TGGTG 3920770 3.9654183 28.74486 25-29
CACTC 2483460 3.9270957 79.37631 25-29
GACCA 2427260 3.901147 103.79238 45-49
CTCAG 2720640 3.863017 28.79678 140-149
CCACT 2437500 3.854419 26.780512 240-247
CCCAG 2886825 3.8458786 79.21074 60-69
TGAGG 3342830 3.8269463 67.11506 35-39
TAGTA 2079470 3.7965524 24.037758 80-89
AGTAG 2386135 3.6702247 25.900562 70-79
CAGGG 3373560 3.6236486 32.72732 50-59
CTCCC 2739320 3.5905108 53.654663 30-34
CTTGG 3177240 3.5787098 37.644695 20-24
CTGAG 2803740 3.5746586 93.99912 35-39
AGGAG 2757540 3.573389 89.54184 35-39
GACGG 3305760 3.5508225 106.80138 40-44
TGTCT 2613650 3.4943228 20.20878 170-179
TCTTG 2583590 3.454134 18.353285 170-179
GCCCC 3126960 3.4530008 70.67769 60-69
ATGGT 2529450 3.437188 23.603754 190-199
ACGGT 2684945 3.4231997 91.05091 40-44
AGGCT 2635720 3.3604398 43.790337 30-34
CTTCA 1982995 3.3420763 15.977508 200-209
GAGGA 2548125 3.3020163 122.74098 35-39
TTCCC 2350690 3.283895 55.55575 50-59
AGATA 1573960 3.2527514 16.897047 150-159
GCCCT 2762930 3.251806 33.502987 20-24
CCTGG 3073775 3.248389 31.89374 50-59
CCAGG 2704215 3.234876 39.234783 45-49
ATGCA 1877240 3.2157044 75.17134 25-29
GGGTT 3178225 3.2144172 43.103455 50-59
TGTCC 2558980 3.209978 18.989822 120-129
TCAGG 2470660 3.1499949 19.040903 140-149
CTCTC 2232470 3.1187425 13.216091 100-109
CCAGT 2177135 3.0912986 40.604443 60-69
GATGG 2697715 3.0884042 32.844536 15-19
GGATG 2667685 3.0540252 57.06957 20-24
CCGTG 2854180 3.0163195 23.178022 120-129
GGTTC 2677275 3.01557 48.553318 50-59
TGCAC 2109845 2.995754 100.270386 25-29
ACAGA 1529090 2.9648986 13.577485 140-149
GACTC 2057395 2.9212804 20.819532 190-199
TGGAG 2549895 2.9191766 31.34505 25-29
TCGCA 2039765 2.8962479 23.501514 100-109
TCTCA 1709080 2.8804288 20.318956 130-139
TACAC 1505135 2.8713791 21.857378 110-119
TGGCC 2677930 2.8300574 37.622658 60-69
TCTCT 1899055 2.8275604 14.353523 180-189
CGCAC 2116115 2.8191257 24.097431 100-109
AGGGT 2434305 2.7868466 50.289967 50-59
GTTCC 2220825 2.7857974 55.207184 50-59
GCCGT 2628405 2.7777188 21.716475 120-129
TTCTT 1742855 2.7657695 20.76708 170-179
CGGAT 2150620 2.7419562 64.36835 20-24
GCACT 1930070 2.7404928 71.92461 25-29
CAGAT 1599070 2.7392006 14.73793 150-159
GGTTG 2698365 2.729093 58.308674 15-19
AGTCA 1588500 2.7210944 28.23779 70-79
CCTGA 1878320 2.6670132 63.495304 30-34
CGGTG 2807805 2.6644294 66.667564 40-44
AGGGA 2051710 2.6587315 9.668065 150-159
TCACA 1376760 2.6264758 12.188915 130-139
ACCAC 1455310 2.6048949 13.818221 230-239
GGCTG 2711945 2.5734642 24.560724 30-34
GTTGG 2498295 2.5267448 44.949886 15-19
AATAA 905255 2.5135512 11.558633 110-119
TCAAA 1092015 2.5133014 51.406082 70-79
CGGCC 2509690 2.4884887 10.190399 120-129
CTGGC 2339770 2.4726872 33.877773 60-69
GATGC 1918425 2.4459167 72.9394 25-29
ACTCT 1422875 2.398068 12.982596 190-199
AGATG 1553815 2.3899946 15.840607 180-189
TGTTC 1766215 2.3613434 12.169597 140-149
GAGAT 1515900 2.3316758 15.324791 180-189
GTCTC 1847300 2.3172486 11.545188 180-189
CAGAG 1580680 2.2811887 8.882706 140-149
GTTCT 1699280 2.2718546 18.09659 160-169
TCCTC 1622240 2.266256 13.936155 130-139
TCCAT 1340410 2.2590842 11.436954 70-79
TTGGT 1877465 2.253869 41.721817 25-29
TTGTC 1679095 2.2448683 14.718602 170-179
CTGGA 1750835 2.2322462 13.803575 180-189
TGTAG 1636225 2.2234137 8.827921 210-219
TCATT 1232815 2.2144866 15.314531 140-149
TTCAT 1226690 2.2034843 16.59873 140-149
GGCGG 2755355 2.202803 35.23894 20-24
CATTT 1225950 2.2021549 16.232372 150-159
ACAGC 1351500 2.172161 11.327223 120-129
GCGGA 2008930 2.157856 75.78969 20-24
TTCAC 1270780 2.141732 15.97834 200-209
ATATA 869080 2.1318474 9.976338 110-119
TCTGC 1698655 2.1307886 10.927197 210-219
TAGTC 1399150 2.117387 24.079515 70-79
GATAT 1150470 2.1004486 9.81778 180-189
TCTCG 1672005 2.097359 16.412674 100-109
GTGAA 1352855 2.0808887 15.380421 190-199
CACTA 1089415 2.0783012 13.333956 240-247
TTGGA 1519475 2.0647657 16.029251 170-179
CTCTG 1640395 2.0577073 14.272439 180-189
TGAAT 1124285 2.0526419 13.525507 190-199
GAGTC 1588720 2.0255558 11.533264 210-219
ATTTG 1255015 2.0242553 14.215303 150-159
GAATC 1180375 2.0219777 14.002449 190-199
TTGGG 1992905 2.0155995 72.27962 15-19
CCTTG 1602840 2.0105984 42.4732 20-24
CAGCC 1507725 2.0086179 10.580395 120-129
GTCAC 1405135 1.9951413 9.377895 130-139
CCTTC 1425280 1.9911046 12.60883 200-209
ATATG 1088025 1.9864409 9.895129 180-189
GTTCA 1311135 1.9841906 14.461168 140-149
AATAT 806775 1.9790137 11.758822 110-119
GAACT 1151270 1.9721209 11.482432 160-169
ACTAC 1032505 1.9697325 10.952889 230-239
CTACT 1168620 1.9695549 7.665261 230-239
CTCGC 1673270 1.9693401 16.327522 100-109
GATAC 1142070 1.9563614 12.571154 150-159
AATAG 943695 1.9502436 7.7369714 240-247
GTCAA 1136090 1.9461179 38.366425 70-79
TCCCA 1224375 1.9361042 12.022766 240-247
ATAGA 932310 1.9267151 9.189806 240-247
TCGGC 1817060 1.9202832 8.178838 190-199
ACACA 887440 1.9163485 12.645748 110-119
CTCCA 1205505 1.9062651 8.503962 240-247
GTTGT 1584970 1.9027331 7.7712536 210-219
GCTGA 1492130 1.9024072 37.858955 30-34
TCAGC 1326055 1.8828561 11.448912 140-149
AATCG 1094230 1.8744117 12.652672 190-199
CACGG 1565765 1.8730224 6.9818816 200-209
GGTCA 1459405 1.8606843 9.870962 130-139
GTCTG 1649575 1.858012 8.332767 210-219
TACAG 1084445 1.8576499 10.577194 160-169
GCTGT 1632195 1.8384355 9.173478 140-149
TTGCA 1214035 1.8372455 12.31435 150-159
GTCCT 1450320 1.8192778 11.797883 130-139
TTCAG 1194975 1.8084012 9.994324 150-159
ATCTC 1064280 1.7937033 6.8157635 100-109
TTTGC 1336060 1.7862471 11.086294 150-159
TGGAA 1160120 1.7844342 9.113841 25-29
TGGGG 2093905 1.7841668 43.563145 15-19
CACCA 995120 1.7811896 10.380024 230-239
CATAG 1038170 1.778381 8.260011 210-219
AGTCT 1172195 1.7739273 12.075315 210-219
ATTGT 1095505 1.7669764 8.005185 170-179
AGTAT 965870 1.7634188 10.944763 220-229
CAGGC 1472605 1.7615813 12.689868 140-149
TACCA 922125 1.7591583 10.745329 230-239
CTGTT 1303085 1.7421612 11.700442 140-149
TACTA 856815 1.742138 9.550149 230-239
TTGTA 1078450 1.7394679 10.160293 210-219
CCAGA 1067765 1.7161359 21.11332 60-69
GTGGA 1492970 1.7091852 38.714703 25-29
ATGTA 933120 1.7036259 9.382663 70-79
ATAGT 932625 1.7027222 6.7576656 220-229
CAGCT 1178630 1.6735284 8.989535 140-149
CCCAC 1127890 1.6734034 10.279737 240-247
TGACT 1100820 1.6659129 11.925119 190-199
GGAGG 1719640 1.6585801 26.842564 30-34
AGAAC 848250 1.6447529 10.495285 150-159
TCTGG 1459290 1.643683 11.312928 180-189
GGGGC 2038450 1.6296643 40.08609 15-19
ACGGC 1340355 1.6033792 11.425038 120-129
CACTG 1128820 1.6028033 6.215336 230-239
TGGTT 1325010 1.590655 16.865295 10-14
TGCAG 1247015 1.5898952 11.524666 150-159
GACGT 1245375 1.5878043 10.082813 170-179
GCAGA 1099095 1.5861801 12.500403 150-159
CTCTT 1060290 1.5786977 8.909252 200-209
GAGAA 905280 1.5761623 10.157633 160-169
ACACG 975640 1.5680703 9.384278 110-119
GGACG 1458140 1.5662348 7.734891 170-179
CGACT 1090620 1.5485637 9.65111 190-199
GGGCG 1933930 1.5461044 46.167725 20-24
ATGGG 1346665 1.5416919 53.251297 15-19
AACTG 899335 1.5405573 10.160971 160-169
ACTGA 897710 1.5377736 7.3874803 180-189
CAAAG 792060 1.5358006 26.731539 70-79
AGCTC 1073910 1.5248371 9.536549 140-149
ATCGG 1193220 1.5213087 8.934987 190-199
ATAAA 543490 1.5090663 7.868114 8
TCGAC 1062745 1.5089842 10.276031 190-199
AACCA 693665 1.4979086 11.033234 70-79
CTTGA 988605 1.4960936 9.44953 200-209
GTCCC 1267195 1.491414 15.053191 50-59
CTGGT 1320040 1.4868375 7.0970917 160-169
TCCAC 937805 1.4829512 10.815658 240-247
TCAGA 861540 1.4758147 8.476366 130-139
CCACC 990540 1.469623 7.74791 230-239
CAAAA 563025 1.4667766 17.886887 70-79
GAGGG 1513685 1.4599379 9.420977 200-209
ACGGA 1003055 1.4475781 9.364337 200-209
TGGAC 1135150 1.4472718 10.678357 170-179
AAATA 513090 1.4246571 5.2899785 6
CCATC 900885 1.4245696 11.185236 240-247
GCTCT 1132345 1.4204108 8.801701 130-139
CTCGA 994835 1.412559 9.258407 190-199
ATCCA 734675 1.4015558 16.85202 240-247
CCTCG 1187665 1.3978117 8.413931 120-129
AGCCG 1158400 1.3857183 10.00884 120-129
TAGTT 858950 1.385429 8.369074 220-229
GGCCG 1542930 1.3737353 11.029729 120-129
GAGGC 1269940 1.3640832 23.52574 30-34
GGGAG 1413750 1.3635513 6.936591 150-159
AATTG 745950 1.3619043 8.944492 170-179
CGTAG 1048735 1.337096 7.75005 210-219
CAATA 579750 1.3343098 7.073891 100-109
TATGG 973530 1.3228985 7.0808234 180-189
ACTAT 639015 1.2992914 6.968824 230-239
GGTGG 1523980 1.2985473 39.00153 25-29
GAATT 710335 1.2968805 9.932633 170-179
CAGAC 806185 1.2957187 10.397205 60-69
GGCTC 1225505 1.2951232 7.9607434 130-139
CATGT 853550 1.2917098 9.315829 70-79
ACTCG 907715 1.288858 9.349786 190-199
GGAAT 835030 1.2843982 7.9408965 170-179
CTGAA 748580 1.2823147 11.866044 35-39
AGATC 748510 1.2821946 6.320461 130-139
TTCCA 755395 1.2731185 7.265846 230-239
ACGTG 995680 1.2694529 9.526804 170-179
ACTTC 752790 1.2687281 5.407187 220-229
GTGTT 1056615 1.2684507 7.144788 160-169
GTGCT 1124845 1.266978 7.1495137 220-229
CCTCA 799055 1.2635458 5.975551 130-139
ACTGT 831195 1.2578791 5.132046 230-239
GGGCC 1405450 1.2513311 29.861536 15-19
CCATG 876895 1.2450969 9.273395 70-79
TGGGC 1307805 1.2410243 32.316532 15-19
ACCAT 648060 1.2363183 9.054608 45-49
GCTCA 870505 1.2360238 9.354503 140-149
TATAC 606555 1.2332915 6.076195 110-119
TGATA 673225 1.2291278 7.1193337 180-189
TCCAG 857365 1.2173665 5.536314 240-247
TTGAG 894960 1.2161324 8.578315 200-209
GAAGA 695395 1.2107363 15.632167 35-39
AAGTA 585085 1.2091389 16.216473 70-79
TGTGC 1070290 1.2055296 5.777454 220-229
AAAGT 579885 1.1983924 19.660025 70-79
GTCCA 843415 1.197559 10.403687 70-79
CTGAT 790630 1.1964906 6.010339 180-189
AGAGA 685470 1.193456 10.865297 35-39
GGAGT 1026180 1.1747937 6.2431746 210-219
AGAGC 810150 1.1691834 6.9382825 140-149
AGTTC 761530 1.1524523 5.3597503 160-169
TATCA 565460 1.1497341 5.2907753 70-79
ACTGG 901675 1.1496001 6.963573 160-169
GCGTA 894735 1.1407521 8.426598 210-219
GTCTA 746165 1.1292 7.4324527 180-189
TAGAT 617160 1.1267681 7.792037 240-247
AAGAG 646130 1.1249623 10.821444 35-39
GAACC 699225 1.12381 8.277778 70-79
GCATA 653630 1.1196656 7.525586 210-219
CCCAA 622675 1.1145412 9.473935 240-247
TGCCC 945010 1.1122211 8.787893 60-69
CTGCG 1042195 1.1013999 5.8022275 210-219
AGGTT 801040 1.0885075 18.608496 10-14
ACAAT 472810 1.0881846 7.934786 100-109
ATATC 535030 1.0878617 5.474298 70-79
GCGGT 1136200 1.078182 6.903804 130-139
TCACG 753155 1.0693995 8.14451 200-209
TCTAC 633315 1.0673689 6.938795 180-189
CCAAT 558570 1.0655962 10.950883 240-247
TATGT 657835 1.0610439 7.1196494 220-229
TCTGT 787620 1.0530096 5.249635 210-219
CTTCT 705075 1.0498075 5.1648126 210-219
AAAAG 446740 1.0450389 11.517707 70-79
ACCCA 575200 1.0295645 11.219955 240-247
TATAA 417640 1.0244682 6.955438 240-247
TGGTC 905680 1.0201198 7.676942 50-59
CGGCT 958990 1.0134681 6.955698 130-139
GTGGT 999595 1.0109781 6.7555947 50-59
TAGGC 791880 1.0096159 5.2640615 160-169
CTCGG 946565 1.0003372 7.05568 130-139
TACTG 655890 0.99258333 5.2559147 180-189
TGTAA 543070 0.9914997 6.581726 230-239
CGGTC 923010 0.97544414 6.9871545 130-139
ATAAC 423385 0.97443175 6.8446074 80-89
GCTTC 771315 0.9675355 5.428469 150-159
CTTCC 683835 0.9553121 5.007319 230-239
AAAAT 343660 0.9542139 5.3105483 5
CATCA 499380 0.95267826 5.473853 230-239
AGCTT 627790 0.9500585 5.465859 150-159
CGATG 741845 0.94582325 22.250786 15-19
CCAGC 706120 0.9407055 5.4539375 80-89
GGAAG 717455 0.92972213 9.507412 30-34
TTCTG 685730 0.9167876 5.1285877 210-219
CGGAG 847955 0.9108156 5.779924 210-219
ACTAA 384640 0.88525903 10.270348 240-247
ACCGT 618075 0.8776004 12.043302 45-49
ACGTC 614780 0.8729217 6.8515196 70-79
TGAAG 566545 0.8714291 8.663733 35-39
AATCC 455715 0.8693776 10.558318 240-247
TGCAT 564365 0.8540752 6.2975807 210-219
AGCTG 658850 0.84000784 7.011932 30-34
GAGCT 656995 0.8376429 5.284713 140-149
CTACC 522170 0.82570755 5.9393916 230-239
ATCAA 357685 0.8232214 11.519784 70-79
TGCGT 715900 0.80635965 6.352146 210-219
AGCCA 497170 0.7990628 7.3182244 80-89
CCTTT 527650 0.785634 5.968095 50-59
AAAGG 443325 0.771863 6.9501615 10-14
CCCAT 485710 0.7680533 5.2348785 240-247
AGGAT 496505 0.7636973 8.422535 10-14
TGAGA 489830 0.7534302 5.861341 240-247
CAATC 393275 0.75025934 10.614567 240-247
TAGGT 543575 0.7386465 5.8700614 10-14
TAACC 384180 0.73290867 6.070845 80-89
GACCG 610020 0.7297271 12.257527 45-49
TGGAT 530225 0.7205057 7.215683 10-14
TCGAA 418725 0.7172743 12.22915 70-79
CGGCG 795515 0.7082804 5.0014434 130-139
ATACC 367420 0.70093524 5.829574 240-247
CTAAT 341755 0.69488096 8.181247 240-247
CATCC 427750 0.67640114 8.067498 240-247
GGTCC 639170 0.6754798 8.550842 50-59
GTCGA 519620 0.66249514 8.367453 70-79
AAGGT 425355 0.6542582 6.373307 10-14
ACGAT 379645 0.65033036 8.0741205 10-14
AATGG 416705 0.64095324 5.049951 10-14
CTTTG 469705 0.6279727 5.1479473 50-59
AAAGC 321675 0.62372637 8.083889 70-79
CGAAC 384475 0.61793685 8.253369 70-79
GAAGC 422575 0.6098471 8.763486 30-34
ATAGG 376820 0.5796042 5.5038576 10-14
AAGCT 329485 0.5644065 14.156255 30-34
CGTCC 476120 0.56036514 5.45321 70-79
TTGCC 444750 0.5578933 6.0877757 60-69
TCAAG 301990 0.5173077 5.4711576 70-79
GACGA 352380 0.50854397 5.9124136 40-44
CGGTT 435645 0.4906922 9.868728 10-14
GGGAT 419245 0.47996095 6.2789607 10-14
TAAAG 224310 0.46355984 5.805101 9
AAGCA 233565 0.45288146 5.005715 70-79
TCGAT 290770 0.44003338 6.988043 10-14
GCGAT 257880 0.3287869 5.390476 10-14