FastQCFastQC Report
Fri 7 Jul 2017
HNCHNBCXY_s1_1_illumina12index_1_SL254619.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_1_SL254619.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2076212
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2318590 7.9593763 56.109898 110-119
CACAG 3047300 6.8422794 38.92751 110-119
TCCCT 3295280 6.3487864 89.69312 30-34
CAGTA 2625925 6.291926 40.932377 60-69
GGAGA 3283750 6.011976 90.14532 35-39
ATACA 1852335 5.9588017 29.945923 110-119
GGTGA 3571500 5.755653 90.824425 45-49
AGTAA 1935460 5.622175 40.981586 110-119
CCCTG 3341515 5.447622 53.468246 30-34
GTAAT 2093025 5.351695 35.779575 110-119
ACAGT 2013085 4.823513 32.404057 110-119
GGCCC 3494920 4.821318 56.02005 60-69
GTAGT 2528065 4.814677 24.495415 70-79
AGACG 2372855 4.8110247 95.23402 40-44
GTGAC 2668175 4.7618694 80.46635 45-49
GAGAC 2329835 4.7238007 124.693016 40-44
CCCCA 2272835 4.6618023 96.17533 60-69
GTGTC 2789850 4.382691 25.238792 120-129
ACTCC 1973935 4.320504 90.48139 30-34
ACCAG 1905500 4.2785296 92.97677 45-49
GCACA 1882300 4.2264376 31.969204 110-119
CGTGT 2668330 4.1917896 24.148748 120-129
AATAC 1298835 4.17824 30.061941 110-119
CCCTT 2160590 4.1626587 64.4104 20-24
TGACC 2074820 4.1007457 146.96405 45-49
TGGTG 2831065 4.015971 29.323336 25-29
TAGTA 1549705 3.9624705 26.463982 80-89
CTCAG 1974865 3.903191 28.895536 140-149
GACCA 1735185 3.8961115 103.81087 45-49
CCCAG 2101750 3.8926713 80.48207 60-69
CCACT 1757175 3.8460653 26.551996 240-247
CACTC 1755540 3.8424864 77.13382 25-29
AGTAG 1764835 3.8184416 26.857813 70-79
TGAGG 2359925 3.803139 67.91633 35-39
AAAAA 849090 3.7619686 18.874514 220-229
CTTGG 2325815 3.653719 38.53477 20-24
GACGG 2392950 3.6137817 109.00306 40-44
AGGAG 1965865 3.5991578 89.479355 35-39
CAGGG 2382290 3.5976832 33.158924 50-59
CTGAG 2005695 3.5795467 95.208275 35-39
CTCCC 1978400 3.5718796 54.97953 30-34
ATGGT 1846110 3.5158997 24.551092 190-199
ACGGT 1954200 3.4876444 91.46755 40-44
GCCCC 2281075 3.4848762 71.47997 60-69
TGTCT 1876865 3.4843998 20.505367 170-179
TCTTG 1864315 3.4610999 18.914814 170-179
AGGCT 1917590 3.4223068 44.238686 30-34
AGATA 1145385 3.3271446 18.200834 150-159
GCCCT 2027380 3.305207 33.70755 20-24
GAGGA 1802995 3.300971 124.31858 35-39
CTTCA 1412140 3.2983367 16.593319 200-209
TGTCC 1892295 3.2920625 19.226995 120-129
CCTGG 2203180 3.2433534 31.455034 50-59
CCAGG 1924045 3.2178273 38.340656 45-49
TTCCC 1669480 3.216471 54.348618 50-59
GATGG 1981280 3.1929333 33.625427 15-19
TCAGG 1788870 3.192581 19.707214 140-149
ATGCA 1332315 3.1923332 76.44579 25-29
GGGTT 2246370 3.1865594 43.03611 50-59
CTCTC 1613955 3.109495 13.338822 100-109
CCGTG 2106335 3.1007855 24.017733 120-129
CCAGT 1542595 3.048838 40.364605 60-69
GGATG 1888060 3.042704 56.439125 20-24
TGGAG 1875360 3.0222378 32.56389 25-29
TGCAC 1517805 2.999842 99.572914 25-29
ACAGA 1089200 2.9649143 13.754644 140-149
GGTTC 1874530 2.9447765 48.1265 50-59
TCGCA 1484505 2.9340267 23.806631 100-109
TCTCA 1249300 2.9179912 20.596046 130-139
TCACA 1085835 2.881272 11.862277 130-139
GACTC 1454965 2.875643 20.3105 190-199
CGCAC 1540535 2.8532398 24.109976 100-109
TACAC 1073130 2.8475597 22.007278 110-119
TCTCT 1382930 2.8432448 15.128041 180-189
TGGCC 1930600 2.8420823 38.185463 60-69
GCCGT 1891595 2.7846618 22.489885 120-129
CAGAT 1161825 2.7838256 15.111717 150-159
TTCTT 1267125 2.7800283 20.961985 160-169
AGGGT 1720505 2.7726812 50.29887 50-59
GTTCC 1586645 2.7603173 54.53872 50-59
GGTTG 1916810 2.7190661 59.01275 15-19
CGGAT 1522335 2.7168984 63.293858 20-24
GCACT 1372235 2.7121325 70.45161 25-29
CGGTG 2031355 2.700293 67.04359 40-44
CCTGA 1365825 2.6994636 62.86862 30-34
AGTCA 1118870 2.6809018 28.616137 70-79
GGCTG 1979380 2.6312025 25.004816 30-34
AGGGA 1429655 2.6174502 9.5944395 150-159
ACCAC 1050800 2.6129127 14.780341 230-239
AATAA 664140 2.5901084 12.328587 110-119
TCAAA 794790 2.5567706 52.472523 70-79
CGGCC 1850790 2.5532053 10.567756 120-129
AGATG 1158285 2.5060947 16.955566 180-189
GTTGG 1764975 2.5036829 45.773838 15-19
CTGGC 1672960 2.4628038 34.18963 60-69
GATGC 1364830 2.4358006 73.808846 25-29
TGTTC 1307765 2.4278657 12.831306 140-149
GAGAT 1115480 2.413481 16.377262 180-189
GTCTC 1362820 2.3709242 12.290129 180-189
ACTCT 1000445 2.3367407 12.803463 200-209
CAGAG 1141965 2.3153636 9.009306 140-149
TCCAT 990585 2.3137107 12.65303 70-79
TTGTC 1236860 2.29623 15.204685 170-179
CTGGA 1286500 2.2960055 14.978619 180-189
TCCTC 1187240 2.2873728 14.481 130-139
GTTCT 1223805 2.2719939 18.133528 160-169
TCATT 911245 2.2712665 15.847976 140-149
TTCAT 910820 2.2702074 17.11493 140-149
TGTAG 1189735 2.2658393 8.5109 210-219
CATTT 907535 2.2620196 16.892298 150-159
TTGGT 1348955 2.2613776 42.42853 25-29
GGCGG 1974780 2.2213082 36.04918 20-24
ACAGC 978680 2.197487 11.72802 120-129
GCGGA 1431655 2.1620548 75.98629 20-24
TTCAC 924620 2.1596363 17.18938 200-209
ATATA 625195 2.1462018 10.266477 110-119
TAGTC 1017470 2.1459506 24.59766 70-79
GTGAA 986815 2.1350982 16.351578 190-199
CACTA 798720 2.1194103 13.972394 240-247
ATTTG 931555 2.0966322 15.015834 150-159
GAGTC 1171100 2.0900524 12.246803 210-219
TGAAT 817165 2.0894246 14.270776 190-199
CTCTG 1198075 2.0843143 14.669294 180-189
TCTGC 1195285 2.0794604 11.171872 210-219
TCTCG 1192690 2.0749457 16.305092 100-109
AATAT 600195 2.0603807 12.243115 110-119
TTGGA 1081795 2.0602684 16.41262 170-179
GAATC 856905 2.053213 14.793937 190-199
GATAT 798040 2.0405235 9.449889 180-189
CCTTG 1170225 2.0358632 43.158142 20-24
CAGCC 1093675 2.025606 10.858936 120-129
CCTTC 1051355 2.0255725 13.324987 200-209
GTTCA 957830 2.0201638 15.419543 140-149
ACTAC 754330 2.0016212 12.181335 230-239
TTGGG 1406580 1.995286 72.36481 15-19
GATAC 830385 1.9896687 13.084292 150-159
TCGGC 1350235 1.9877129 8.68312 190-199
GTCAA 829030 1.9864221 38.786205 70-79
CTCGC 1218055 1.9857767 16.279995 100-109
ATATG 774370 1.9800014 9.414203 180-189
CTACT 842925 1.9688209 8.184442 230-239
AATAG 673615 1.9567347 8.183497 240-247
GAACT 813590 1.9494267 11.0260935 160-169
ATAGA 671030 1.9492257 9.355195 240-247
GTCAC 976870 1.9307195 9.19164 130-139
GCTGA 1080120 1.927681 38.44023 30-34
TCCCA 877480 1.9206084 11.245193 240-247
CACGG 1144970 1.9148802 7.474712 200-209
AATCG 798850 1.9141084 13.376646 190-199
GTTGT 1133680 1.9004925 7.409144 210-219
TTGCA 895950 1.889652 13.231994 150-159
ACACA 626525 1.8886962 13.214227 110-119
GCTGT 1198480 1.8827417 9.7786 140-149
TTTGC 1007745 1.8708782 11.641711 150-159
TACAG 777510 1.8629761 11.059539 160-169
GGTCA 1040840 1.8575783 9.606857 130-139
TGGAA 857190 1.8546382 9.058569 25-29
GTCCT 1063425 1.8500609 12.2797985 130-139
TACCA 692470 1.8374751 11.472806 230-239
AGTCT 866755 1.8280768 12.579991 210-219
GTCTG 1160995 1.823855 8.705244 210-219
CTCCA 831925 1.8208987 8.439132 240-247
TCAGC 918420 1.8151966 11.209665 140-149
AGTAT 708785 1.8123058 11.43577 220-229
ATTGT 802475 1.8061144 8.468944 170-179
ATGTA 698290 1.7854711 10.688889 70-79
CAGGC 1065715 1.782332 13.460883 140-149
CTGTT 958135 1.7787774 12.473644 140-149
TGGGG 1478835 1.7751112 43.274014 15-19
CATAG 740330 1.7738898 7.952225 210-219
TACTA 623415 1.7652793 10.555889 230-239
TTCAG 828190 1.7467393 9.851125 150-159
CCAGA 774845 1.7398044 22.285866 60-69
GTGGA 1078260 1.7376705 39.84191 25-29
TTGTA 768415 1.7294563 9.84668 210-219
ATCTC 735890 1.7188194 6.618836 100-109
CACCA 686780 1.7077428 10.481181 230-239
ATAGT 666035 1.7029974 7.037616 220-229
TCTGG 1069385 1.679941 12.048189 180-189
CCCAC 808535 1.6583827 10.253819 240-247
CAGCT 836405 1.6530997 8.89801 150-159
AGAAC 606550 1.6510913 10.418402 160-169
GCAGA 813825 1.6500511 13.302649 150-159
GGAGG 1208835 1.648451 27.466923 30-34
GGGGC 1442490 1.6225682 39.666985 15-19
TGACT 769215 1.6223547 11.775325 190-199
GACGT 905820 1.6166093 9.944626 170-179
TGCAG 904120 1.6135752 12.494104 150-159
ACGGC 964440 1.6129568 11.76511 120-129
ATAAA 404330 1.5768641 6.770037 8
CACTG 794730 1.5707318 6.491542 230-239
ATGGG 972555 1.5673218 54.698524 15-19
GGACG 1036780 1.5657227 7.7059507 170-179
GTCCC 959980 1.5650408 16.44808 50-59
TGGTT 931185 1.5610315 16.891846 10-14
GAGAA 634215 1.5589126 10.2842455 160-169
ATAAT 453405 1.5564723 5.537972 100-109
GGGCG 1372490 1.5438297 46.845104 20-24
AACTG 642980 1.5406313 10.1118145 160-169
CAAAG 562400 1.5309105 26.74412 70-79
CTCTT 742540 1.5266306 8.882579 200-209
ACACG 677895 1.5221169 8.946707 110-119
TCAGA 632510 1.5155443 8.976925 130-139
ATCGG 847935 1.5133024 9.5167465 190-199
CAAAA 414075 1.5132881 18.761852 70-79
ACTGA 630845 1.511555 7.0288467 180-189
AACCA 500695 1.5093741 11.395617 70-79
CGACT 762180 1.5063988 9.205808 190-199
ACGGA 741500 1.5034103 9.890242 200-209
AGCTC 759340 1.5007857 9.163047 140-149
TCGAC 751805 1.4858932 9.670457 190-199
CCTCG 900345 1.4678189 8.9089985 130-139
CTGGT 931480 1.4633005 6.8275414 160-169
TCCAC 666840 1.4595643 10.710672 240-247
CCACC 702360 1.4406075 7.5780373 230-239
GCTCT 827665 1.4399047 9.09446 130-139
TGGAC 800535 1.4287081 10.624708 170-179
CTTGA 677265 1.4284226 9.331553 200-209
CCATC 651980 1.4270391 11.284724 240-247
GAGGG 1040775 1.4192729 9.224917 200-209
ATCCA 533560 1.415806 16.627111 240-247
GGCCG 1130995 1.4088671 11.410479 120-129
AGCCG 836150 1.3984009 10.51087 120-129
AATTG 542180 1.3863105 9.041083 170-179
TAGTT 614825 1.3837745 8.302356 220-229
CTCGA 700000 1.383504 8.818071 190-199
CGTAG 769050 1.372517 8.162036 210-219
GAGGC 900065 1.3592589 23.854816 30-34
CAGAC 604035 1.356275 11.526934 60-69
CATGT 641540 1.3530749 10.267406 70-79
TATGG 704380 1.3414851 6.916317 180-189
GGGAG 978165 1.3338934 6.8042383 150-159
CAATA 411780 1.3246607 7.5095267 100-109
TCACG 669805 1.3238255 8.717465 200-209
GAATT 510420 1.3051026 10.0956545 170-179
GGCTC 883875 1.3011732 8.313799 130-139
GTGTT 775595 1.3002015 7.4256244 160-169
GGTGG 1075850 1.2913903 40.097374 25-29
GTGCT 817530 1.2842917 7.3220863 220-229
CCATG 649140 1.2829826 10.338541 70-79
ACTAT 452300 1.2807453 6.743613 230-239
ACTGT 607085 1.2804056 5.2341514 230-239
ACGTG 715795 1.2774733 9.361321 170-179
ACCAT 480890 1.2760458 9.8298235 45-49
GGAAT 586970 1.269983 8.280877 170-179
TATAC 446450 1.2641803 6.297983 110-119
CTGAA 527385 1.2636566 12.55301 35-39
GGGCC 1014410 1.2636386 30.775719 15-19
TTCCA 539455 1.2600058 7.055086 230-239
CATCA 472150 1.2528541 5.701992 230-239
ACTTC 535160 1.249974 5.575133 220-229
ACTCG 631390 1.247901 8.954316 190-199
TGTGC 793440 1.2464478 6.3855314 220-229
AGAGA 503740 1.2382026 12.428936 35-39
GTCCA 625080 1.2354296 11.614142 70-79
GGAGT 766595 1.2354066 6.6175346 210-219
TGGGC 927205 1.2325395 33.19739 15-19
CCTCA 562960 1.2321942 5.860461 130-139
GCTCA 622785 1.2308937 9.109105 140-149
AGATC 512560 1.2281348 6.052685 130-139
TGATA 478955 1.2246492 6.8004975 180-189
AAGTA 417045 1.2114433 15.76122 70-79
TTGAG 635260 1.2098467 8.551173 200-209
AGTTC 566405 1.1946073 5.7553616 160-169
AAAGT 411070 1.1940869 19.529808 70-79
GAACC 524665 1.1780608 8.485119 70-79
CTGAT 558015 1.1769118 5.844706 180-189
GAAGA 477870 1.1746136 17.133188 35-39
TGCCC 717080 1.1690447 9.456811 60-69
GCGTA 654655 1.1683573 9.199266 210-219
TATCA 405910 1.149386 5.1913953 70-79
TCCAG 581260 1.1488223 5.6491804 240-247
TAGAT 445520 1.1391585 7.8262143 240-247
ACTGG 630585 1.1253997 6.749487 160-169
GTCTA 531830 1.1216849 7.363194 180-189
AGAGC 549705 1.114541 6.4700937 140-149
CTGCG 753815 1.1097089 6.353772 210-219
GCATA 462575 1.1083666 7.194532 210-219
AAGAG 448810 1.1031835 12.26808 35-39
ACAAT 342065 1.1003935 8.398165 100-109
CCCAA 437370 1.0875615 9.35479 240-247
TATGT 480125 1.0806078 7.0825896 220-229
AGGTT 560020 1.066553 18.43873 10-14
TGGTC 675345 1.0609274 8.5798235 50-59
GCGGT 796750 1.0591248 6.966904 130-139
AAAAG 318300 1.0504128 12.014463 70-79
TCTAC 447895 1.0461489 6.7666216 180-189
CCAAT 394150 1.0458804 10.430859 240-247
GTGGT 736495 1.0447457 7.6150393 50-59
CTCGG 709495 1.0444643 7.226481 130-139
CGGCT 706850 1.0405706 7.3975787 130-139
TCTGT 560020 1.0396769 5.3434467 210-219
ACCCA 416770 1.0363377 11.18076 240-247
ATATC 361565 1.0238175 5.3106213 70-79
TATAA 294775 1.011919 6.551941 240-247
ATAAC 312685 1.0058806 6.764483 80-89
CTTCT 479535 0.9859034 5.1526036 210-219
GCCAC 529490 0.98067355 5.050459 80-89
CGATG 547615 0.9773239 22.99075 15-19
GTCAG 545985 0.97441477 5.2729483 130-139
CGGTC 661320 0.9735449 6.9999266 130-139
CGGAG 638210 0.96381104 6.085984 210-219
GAGTT 506010 0.9636914 5.3541584 160-169
TGTAA 373190 0.95421666 6.350859 230-239
GCTTC 547640 0.95273983 5.351031 150-159
AGCTT 449105 0.94720936 5.532238 150-159
TGAAC 391895 0.9390117 5.3852916 190-199
CCAGC 506405 0.9379176 5.793974 80-89
ACTAA 290000 0.9329049 11.191579 240-247
ACCGT 467650 0.9242796 13.059936 45-49
ACGAT 382840 0.9173153 9.389194 10-14
TAGAC 376760 0.90274704 5.0732694 110-119
TGAAG 413760 0.89522165 9.087383 35-39
GGAAG 488010 0.8934615 9.963483 30-34
AATCC 332320 0.88181394 10.147814 240-247
CTACC 397135 0.86924005 6.3304296 230-239
ACGTC 438715 0.8670914 7.743656 70-79
TGCAT 409600 0.8638891 5.9079347 210-219
AGACC 384100 0.862442 5.1707325 240-247
GTATG 449670 0.8563924 5.0003133 220-229
AGCTG 471450 0.8413929 7.041699 30-34
TTCTG 453110 0.84119856 5.3106537 210-219
GAGCT 470575 0.83983123 5.162468 140-149
ATCAA 257475 0.8282748 11.831671 70-79
TGCGT 522285 0.8204791 6.8945704 210-219
CCTTT 395130 0.8123704 6.290167 50-59
CACAA 268590 0.8096802 5.024356 100-109
AAAGG 315045 0.7743866 7.2204385 10-14
AGCCA 344855 0.7743229 7.1980205 80-89
CCCAT 353420 0.7735578 5.27999 240-247
TGAGA 354335 0.76664823 5.685237 240-247
AGGAT 347755 0.7524116 8.750248 10-14
CTAAT 264010 0.7475781 8.837954 240-247
GACCG 443100 0.741053 13.050873 45-49
TAACC 279180 0.7408065 6.284892 80-89
CAATC 277415 0.7361231 10.180583 240-247
TCGAA 299170 0.7168352 12.396094 70-79
TAGGT 375800 0.71570766 6.2147474 10-14
GGTCC 478520 0.7044406 9.448126 50-59
GCGGC 559625 0.6971183 5.013928 130-139
TGGAT 362260 0.68992084 6.9417996 10-14
ATACC 259770 0.6893019 5.6645727 240-247
AATGG 309600 0.6698584 5.3880963 10-14
GTCGA 375090 0.66941994 8.657048 70-79
CATCC 305385 0.6684198 7.9181266 240-247
AAGGT 306295 0.6627076 6.6585865 10-14
CGAAC 289345 0.6496832 8.615425 70-79
CGTGG 483955 0.64332443 5.036384 50-59
CTTTG 344840 0.6401953 5.529782 50-59
AAAGC 230650 0.6278529 8.41121 70-79
TAAAA 154475 0.60244375 5.1136413 8
GAAGC 292045 0.59212875 9.027218 30-34
CGTCC 356935 0.5819057 6.0253067 70-79
TTGCC 329095 0.5725329 6.338485 60-69
ATAGG 262400 0.5677353 5.112414 10-14
AAGCT 228620 0.5477918 14.725944 30-34
TCAAG 226645 0.54305947 5.9168353 70-79
GACGA 248950 0.5047525 5.908722 40-44
CGGTT 305205 0.47945917 9.653283 10-14
TAAAG 162760 0.47278956 5.099965 9
AAGCA 172800 0.47037932 5.2897353 70-79
GGGAT 291650 0.47000873 6.4510612 10-14
TCGAT 201625 0.42524815 6.789219 10-14
GCGAT 176745 0.31543532 5.1028366 10-14