FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_15_SL254625.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_15_SL254625.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6153206
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7054120 8.1802 57.557796 110-119
CACAG 9171820 7.0311103 40.278526 110-119
TCCCT 9772745 6.402019 91.847145 30-34
CAGTA 7857875 6.3744073 41.904526 60-69
ATACA 5611265 6.1491547 31.721457 110-119
GGAGA 9878305 6.072367 90.991035 35-39
GGTGA 10671305 5.738336 91.30581 45-49
AGTAA 5830860 5.721908 43.00827 110-119
CCCTG 9904705 5.4907203 54.738335 30-34
GTAAT 6328745 5.432738 37.270477 110-119
GGCCC 10463745 4.90865 57.270794 60-69
ACAGT 6041430 4.9008846 33.245266 110-119
AGACG 7113775 4.883398 98.19211 40-44
GTAGT 7562090 4.8053284 24.424452 70-79
GTGAC 7988135 4.796896 82.211235 45-49
CCCCA 6696420 4.73897 99.48469 60-69
GAGAC 6894820 4.7330914 127.08276 40-44
GTGTC 8384620 4.4044437 25.575283 120-129
ACCAG 5665485 4.3431563 94.98306 45-49
AATAC 3942095 4.3199797 31.561611 110-119
ACTCC 5708170 4.2746887 91.40329 30-34
CGTGT 8080930 4.244915 24.573019 120-129
GCACA 5514855 4.227683 32.92196 110-119
CCCTT 6357280 4.164586 64.52798 20-24
TGACC 6207735 4.1628847 150.13568 45-49
GACCA 5196825 3.9838815 105.888535 45-49
TGGTG 8465585 3.9821563 29.0563 25-29
TAGTA 4606070 3.9539545 25.488892 80-89
CCCAG 6205045 3.9322379 82.55585 60-69
CCACT 5211060 3.9024172 27.535145 240-247
CTCAG 5761755 3.863812 29.162153 140-149
CACTC 5144720 3.8527367 78.26458 25-29
TGAGG 7132600 3.8354504 69.31618 35-39
AGTAG 5190770 3.770684 27.180542 70-79
CAGGG 7236435 3.6772804 33.61645 50-59
CTCCC 5929295 3.6706 56.446026 30-34
CTTGG 6977500 3.6652827 37.68178 20-24
GACGG 7155845 3.6363275 110.195724 40-44
CTGAG 6018605 3.6141872 96.519745 35-39
AGGAG 5868225 3.6073008 90.599236 35-39
GCCCC 6778575 3.5510738 73.39212 60-69
TGTCT 5697520 3.5367656 20.679428 180-189
ACGGT 5870665 3.525349 92.15181 40-44
TCTTG 5669650 3.5194647 19.31528 170-179
ATGGT 5522780 3.5094495 24.969353 190-199
AGATA 3442500 3.3781755 18.189003 150-159
AGGCT 5607830 3.3675158 44.337517 30-34
GAGGA 5429390 3.337541 126.63761 35-39
GCCCT 5963225 3.3057413 34.22759 20-24
TGTCC 5625495 3.3000066 19.705011 120-129
CTTCA 4154565 3.2923007 16.833757 200-209
CCAGG 5798110 3.2902958 39.311832 45-49
TTCCC 4973825 3.258299 56.28158 50-59
GGGTT 6835235 3.21525 43.221054 50-59
TCAGG 5345170 3.2097878 19.698864 140-149
ATGCA 3935420 3.1924627 77.12555 25-29
CCTGG 6428395 3.1911256 32.04889 50-59
GATGG 5918530 3.182602 33.52213 15-19
CTCTC 4826970 3.1620955 13.9888 100-109
CCGTG 6293960 3.1243906 24.286585 120-129
ACAGA 3293685 3.0543861 14.713797 140-149
TCGCA 4509280 3.0239067 24.937979 100-109
GGATG 5616925 3.0204184 55.90669 20-24
CCAGT 4496775 3.0155213 41.23811 60-69
GGTTC 5722030 3.005784 48.84603 50-59
GACTC 4479120 3.0036817 21.136206 190-199
TGCAC 4446610 2.9818804 100.68074 25-29
CGCAC 4686675 2.970022 25.346596 100-109
TGGAG 5502275 2.9587672 31.658976 25-29
TACAC 3228475 2.9246812 22.975328 110-119
TCTCA 3683990 2.9193916 20.722565 130-139
TCACA 3192155 2.8917787 12.59551 130-139
TGGCC 5805620 2.8819733 39.035675 60-69
TCTCT 4144485 2.8730114 15.386303 180-189
AAAAA 1879330 2.8479896 13.696298 230-239
TTCTT 3855690 2.8283637 21.324757 170-179
GTTCC 4794200 2.8123555 55.655388 50-59
AGGGT 5173800 2.782134 50.661694 50-59
GCCGT 5597480 2.77865 23.177454 120-129
CCTGA 4105410 2.7530732 64.342445 30-34
GGTTG 5812795 2.7343009 57.81986 15-19
GCACT 4074365 2.7322543 70.97108 25-29
CAGAT 3367875 2.7320628 15.335271 150-159
CGGTG 6139610 2.7292023 67.0483 40-44
CGGAT 4507515 2.706774 63.224396 20-24
AGGGA 4391115 2.6992953 10.008214 150-159
ACCAC 3117695 2.6689987 15.182857 230-239
TCAAA 2422740 2.654981 54.78958 70-79
AATAA 1992335 2.641135 13.001528 110-119
AGTCA 3243750 2.631371 29.582136 70-79
CGGCC 5589435 2.6220613 11.20543 120-129
GGCTG 5785210 2.5716631 24.889004 30-34
AGATG 3438610 2.4978783 17.04331 180-189
CTGGC 5008435 2.4862418 34.89004 60-69
GTTGG 5281345 2.4843102 45.284336 15-19
TGTTC 3944355 2.448479 12.978401 140-149
GATGC 4046265 2.4297922 73.27452 25-29
ACTCT 3039490 2.4086554 13.205611 200-209
GAGAT 3304810 2.4006832 16.725777 180-189
GTCTC 4002050 2.3476675 12.352275 180-189
TTGTC 3726395 2.3131793 15.38823 170-179
GTTCT 3723960 2.311668 18.668867 160-169
CAGAG 3364320 2.3095071 9.650462 140-149
CATTT 2732925 2.2917545 16.942429 150-159
TCATT 2725935 2.2858925 16.164148 140-149
TTCAT 2706335 2.2694564 17.745312 140-149
ACAGC 2948870 2.2606013 12.096121 110-119
CTGGA 3764415 2.2605405 15.200609 180-189
TCCTC 3447375 2.258338 14.476481 130-139
TGTAG 3532870 2.2449613 9.164845 210-219
ATATA 1918075 2.2242656 10.3971 110-119
TTGGT 3999015 2.2229364 41.850876 25-29
TCCAT 2798365 2.2175748 11.917505 70-79
GTCAA 2728340 2.213264 40.20887 70-79
GGCGG 5848210 2.199911 35.93219 20-24
CACTA 2399970 2.174137 14.264494 240-247
GTGAA 2985815 2.1689587 16.97833 190-199
TAGTC 3047460 2.1625473 24.747375 70-79
TTCAC 2727040 2.1610532 17.295975 200-209
GCGGA 4215480 2.142146 76.25801 20-24
TGAAT 2465040 2.116046 14.635401 190-199
TTGGA 3327875 2.1146972 17.073275 170-179
ATTTG 2797300 2.10055 14.845163 150-159
AATAT 1809145 2.0979464 12.505652 110-119
GAATC 2583700 2.09593 15.062558 190-199
GAGTC 3456550 2.075667 12.439692 210-219
CTCTG 3520555 2.0652144 14.893807 180-189
TCTGC 3519270 2.0644608 10.865207 210-219
CAGCC 3256495 2.0636935 10.970742 120-129
ACTAC 2266480 2.0532084 12.072521 230-239
TCTCG 3484610 2.0441287 16.715496 100-109
CTACT 2579075 2.0437977 8.492507 230-239
AATAG 2081800 2.0429008 8.482186 240-247
GATAT 2378405 2.0416765 9.677387 180-189
CCTTG 3476480 2.0393596 42.575466 20-24
CTCGC 3677490 2.0386336 17.221338 100-109
GTTCA 2852540 2.0242276 15.757186 140-149
ATAGA 2059540 2.0210567 10.3908825 240-247
CCTTC 3081840 2.0188801 13.658217 200-209
ATATG 2349690 2.017027 9.7096195 180-189
GATAC 2481000 2.0126188 13.315254 150-159
TCGGC 4044785 2.0078754 8.885256 190-199
TTGGG 4212095 1.981342 71.66178 15-19
CACGG 3474615 1.9717652 7.6386786 200-209
ACACA 1879790 1.9466937 13.813139 110-119
TCCCA 2588250 1.9382677 11.497204 240-247
GTTGT 3485095 1.9372631 8.076506 210-219
AATCG 2385185 1.9348923 13.75074 190-199
GCTGA 3203815 1.9238989 38.636623 30-34
GAACT 2361380 1.9155815 11.798619 160-169
GTCAC 2848520 1.9102072 9.485129 130-139
GGTCA 3163045 1.8994164 10.103865 130-139
TACAG 2335255 1.8943884 11.334273 160-169
TTGCA 2665170 1.8912656 13.3637085 150-159
TGGAA 2564095 1.8626125 9.209972 25-29
GTCCT 3173070 1.8613741 12.380864 130-139
TACCA 2045040 1.8526053 11.657095 230-239
ATTGT 2462920 1.8494571 8.449737 170-179
TTTGC 2978545 1.8489475 11.489046 150-159
GCTGT 3512285 1.8450044 9.856105 140-149
AGTAT 2130260 1.8286631 11.674293 220-229
AGTCT 2541435 1.8034602 13.072952 210-219
TACTA 1881015 1.8031828 10.695723 230-239
CTGTT 2887090 1.7921764 12.498352 140-149
TTGTA 2385335 1.791197 10.619773 210-219
TCAGC 2651505 1.7780896 11.391535 140-149
GTCTG 3378585 1.7747719 8.852455 210-219
CATAG 2184270 1.7719076 8.223263 210-219
CAGGC 3103235 1.7610152 13.522901 140-149
AGAAC 1881335 1.7446488 11.292835 160-169
ATGTA 2027690 1.7406149 10.311584 70-79
TGGGG 4365630 1.737783 42.562553 15-19
TTCAG 2430525 1.724756 10.575365 150-159
GTGGA 3193965 1.7175075 39.07687 25-29
ATAGT 1978520 1.6984061 7.0578837 220-229
TCTGG 3223995 1.6935655 12.322081 180-189
CACCA 1977965 1.6932979 11.056707 230-239
CTCCA 2248330 1.6837112 8.638596 240-247
CCCAC 2369350 1.6767584 10.533785 240-247
ATCTC 2109565 1.6717329 6.937624 100-109
ACGGC 2937950 1.6672196 12.396507 120-129
CAGCT 2479165 1.662519 9.057372 150-159
GGAGG 3635015 1.6541005 26.866709 30-34
TGACT 2330630 1.6538683 12.246378 190-199
CCAGA 2156760 1.6533705 22.501942 60-69
GCAGA 2403035 1.6496131 13.427499 150-159
GACGT 2738960 1.6447523 10.490811 170-179
GAGAA 1978350 1.6428528 11.211147 160-169
AACTG 1998400 1.6211274 11.087671 160-169
CGACT 2403575 1.6118287 9.975562 190-199
ATAAT 1388395 1.6100304 5.554821 110-119
TGCAG 2679975 1.6093316 12.497611 150-159
GGACG 3166550 1.6091201 8.32318 170-179
CACTG 2396975 1.6074027 6.880565 230-239
GGGGC 4252305 1.5995823 39.297073 15-19
TGGTT 2841665 1.5795994 16.262087 10-14
AACCA 1524580 1.5788413 12.181607 70-79
ACTGA 1944380 1.5773057 7.8677588 180-189
CTCTT 2269070 1.572949 9.4144945 200-209
TCGAC 2341705 1.5703388 10.646846 190-199
CAAAG 1689405 1.5666633 28.605825 70-79
ATAAA 1178070 1.5617062 6.487747 110-119
ATGGG 2856960 1.536288 53.8306 15-19
GTCCC 2770685 1.535942 16.334991 50-59
GGGCG 4076795 1.533561 46.2259 20-24
ACGGA 2230735 1.5313343 10.1473 200-209
CAAAA 1211255 1.5173906 19.19791 70-79
AGCTC 2241895 1.5034066 9.610241 140-149
ACACG 1952115 1.4964898 9.445892 110-119
CCTCG 2676980 1.4839962 9.059949 130-139
CTGGT 2801930 1.4718547 7.3807707 160-169
TCAGA 1805910 1.464977 8.764349 130-139
GAGGG 3215860 1.4633654 9.789285 200-209
CTCGA 2178220 1.4607064 9.51901 190-199
AAATA 1100465 1.4588293 5.9102364 6
ATCGG 2428245 1.4581672 9.76626 190-199
TGGAC 2425360 1.456435 11.353908 170-179
TCCAC 1943805 1.4556609 10.365575 240-247
GCTCT 2457430 1.4415681 9.108567 130-139
CCACC 2029565 1.4362969 8.230871 230-239
GGCCG 3418780 1.4361495 12.029421 120-129
CATGT 2022515 1.4352229 9.785289 70-79
AATTG 1665325 1.4295524 9.311403 170-179
CTTGA 2006515 1.4238687 9.821954 200-209
AGCCG 2488455 1.4121413 10.792028 120-129
CCATC 1873425 1.4029557 10.514472 240-247
GGGAG 3071300 1.3975841 7.128617 150-159
TAGTT 1860890 1.3973805 8.645747 220-229
GAATT 1584405 1.360089 10.353073 170-179
CGTAG 2260760 1.357592 8.164358 210-219
CAATA 1231150 1.3491665 7.352679 100-109
GAGGC 2625070 1.3339604 23.670906 30-34
CAGAC 1738690 1.3328784 11.527849 60-69
ACTCG 1987415 1.3327533 9.899843 190-199
TATGG 2096435 1.3321791 6.897955 180-189
TATAC 1378795 1.3217435 6.5175667 110-119
ATCCA 1458045 1.3208456 16.37178 240-247
ACTAT 1376740 1.3197737 7.0402646 230-239
ACTGT 1858055 1.3185183 5.522444 230-239
ACGTG 2192230 1.3164396 10.156951 170-179
GTGCT 2468535 1.2967223 7.5886087 220-229
GGCTC 2603605 1.2924579 8.177831 130-139
ACCAT 1426495 1.2922643 9.405607 45-49
TTCCA 1613465 1.2785965 7.4322524 230-239
GGTGG 3209290 1.2774899 39.226246 25-29
AAAGT 1293155 1.268992 21.236328 70-79
TGATA 1474345 1.265611 7.2552843 180-189
GGGCC 3003195 1.2615721 30.326841 15-19
AAGTA 1281660 1.2577118 17.16861 70-79
GGAAT 1727640 1.2549939 8.364281 170-179
ACTTC 1583080 1.2545179 5.5082693 220-229
GTGTT 2255475 1.2537533 7.2866125 160-169
GCTCA 1855985 1.2446167 9.478811 140-149
TTGAG 1941615 1.2337987 8.945553 200-209
CCATG 1830310 1.2273993 9.754381 70-79
TGGGC 2756760 1.2254453 32.515465 15-19
AGAGA 1469410 1.220221 11.940013 35-39
GGAGT 2267615 1.2193763 6.9771423 210-219
CCTCA 1628025 1.2191825 5.46208 130-139
TGTGC 2309260 1.213055 6.1565394 220-229
CTGAT 1703630 1.2089347 6.3614774 180-189
GTCCA 1802140 1.2085085 11.106863 70-79
GAACC 1565840 1.2003716 8.892335 70-79
CTGAA 1465765 1.1890471 13.349437 35-39
GCGTA 1967165 1.1812875 9.21016 210-219
TATCA 1224775 1.1740965 5.51525 70-79
ACTGG 1944445 1.167644 7.470591 160-169
AGTTC 1626925 1.1545032 5.7675395 160-169
GTCTA 1625485 1.1534814 7.7445793 180-189
TCACG 1713580 1.1491206 8.786936 200-209
CTGCG 2291185 1.1373693 6.299436 210-219
AGATC 1392630 1.1297191 5.5016646 130-139
TAGAT 1310960 1.1253576 8.337599 240-247
GCATA 1383015 1.1219193 7.6836686 210-219
TGCCC 2000760 1.1091305 9.258372 60-69
GAAGA 1328365 1.103095 17.305994 35-39
ACAAT 1004840 1.1011628 8.405518 100-109
TATGT 1464010 1.0993551 7.463531 220-229
ATCAT 1145575 1.0981737 5.1285157 90-99
AGGTT 1719595 1.0927162 18.082712 10-14
CCCAA 1272480 1.0893457 9.684342 240-247
TCTAC 1374135 1.0889385 7.3303213 180-189
AGAGC 1584645 1.0878123 6.762885 140-149
GCGGT 2437375 1.083471 7.391402 130-139
CCAAT 1172855 1.0624914 10.876809 240-247
CGGCT 2117340 1.0510706 7.3306594 130-139
AAAAG 932110 1.045641 12.410582 70-79
TATAA 899715 1.0433403 7.2644343 240-247
CTCGG 2099975 1.0424505 7.439185 130-139
AAGAG 1252970 1.0404859 11.719566 35-39
TCCAG 1540150 1.032819 5.6164126 240-247
ATAAC 941400 1.0316416 7.0427995 80-89
GTGGT 2189470 1.0299125 7.1529784 50-59
TGGTC 1955180 1.0270567 8.115754 50-59
CGGTC 2044170 1.0147483 7.5141106 130-139
TGTAA 1171895 1.0059811 6.640997 230-239
TACTG 1416715 1.0053334 5.393745 180-189
ATATC 1044520 1.0013001 5.665575 70-79
ACCCA 1163045 0.9956605 11.277034 240-247
GCCAC 1564630 0.9915314 5.0310874 80-89
TCTGT 1593080 0.9889127 5.2918806 210-219
CGATG 1640535 0.98514533 23.425 15-19
CATCA 1087145 0.9848466 5.787318 230-239
GTCAG 1618560 0.9719493 5.043811 130-139
AGCTT 1366145 0.9694476 5.7567883 150-159
CGGAG 1894940 0.9629363 6.513647 210-219
GCTTC 1640735 0.9624818 5.4665246 150-159
ACTAA 865205 0.9481426 10.928959 240-247
GAGTT 1476990 0.9385529 5.1570754 160-169
TAGAC 1146420 0.9299906 5.0188966 110-119
ACCGT 1383230 0.9275891 13.006302 45-49
CCAGC 1451450 0.9198075 5.973482 80-89
CAATC 1006970 0.9122158 10.682427 240-247
CTTCC 1389895 0.9105051 5.1086493 230-239
TGAAG 1235635 0.89759123 9.531846 35-39
CTACC 1176435 0.8809991 6.434161 230-239
TGCAT 1227935 0.8713707 6.329875 210-219
AATCC 956595 0.86658114 10.478398 240-247
TGAAC 1061925 0.86144716 5.262105 190-199
GAGCT 1432710 0.8603459 5.5912833 140-149
AGCTG 1414565 0.8494498 7.346287 30-34
ATCAA 774945 0.84923035 12.193685 70-79
TGCGT 1591020 0.8357633 6.85564 210-219
AGACC 1083615 0.8306984 5.5000224 240-247
GGAAG 1339945 0.82368773 10.405095 30-34
AGCCA 1059540 0.81224245 7.629592 80-89
GTATG 1276190 0.8109547 5.0414395 220-229
CCTTT 1161055 0.8048586 6.102175 50-59
ACGTC 1168325 0.7834745 7.586298 70-79
AAAGG 935200 0.7766047 7.7023525 10-14
TGAGA 1051155 0.76358104 6.2199793 240-247
TAACC 829285 0.75125074 6.17548 80-89
TCGAA 912140 0.7399395 12.955155 70-79
CGGCG 1759690 0.73920465 5.2270813 130-139
CTAAT 768345 0.7365526 8.699055 240-247
ATACC 812885 0.736394 6.2333393 240-247
TAGGT 1140590 0.7247877 5.7116528 10-14
GCGGC 1707760 0.71739006 5.245293 130-139
AGGAT 985870 0.7161566 8.357906 10-14
TGGAT 1122070 0.71301913 7.146784 10-14
GACCG 1243020 0.70538545 13.413268 45-49
ACGAT 858520 0.6964423 8.784623 10-14
GTCGA 1157900 0.69532186 8.849733 70-79
GGTCC 1383055 0.6865636 9.15341 50-59
AATGG 916335 0.6656449 5.641416 10-14
CGAAC 864045 0.66237617 8.919501 70-79
AAGGT 902180 0.6553625 6.878582 10-14
CTTTG 1019170 0.6326551 5.3068366 50-59
CATCC 834695 0.6250797 6.8197126 240-247
AAAGC 669195 0.6205754 8.620313 70-79
GAAGC 866100 0.5945522 9.647314 30-34
CGTCC 1055890 0.58533746 5.9662743 70-79
ATAGG 802215 0.58274573 5.4900217 10-14
TTGCC 969305 0.56861 6.200181 60-69
AAGCT 689735 0.55952185 15.023556 30-34
TCAAG 676615 0.5488786 5.789499 70-79
GACGA 739995 0.5079849 6.452903 40-44
GGGAT 899595 0.48374388 6.1108785 10-14
TAAAG 488695 0.47956353 5.1807795 9
CGGTT 896590 0.470979 9.418403 10-14
AAGCA 483120 0.44801953 5.1755767 70-79
TCGAT 606675 0.4305104 6.7538624 10-14
GCGAT 558950 0.33565086 5.2319007 10-14