FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_18_SL254628.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_18_SL254628.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2806238
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3203950 8.182824 57.995365 110-119
CACAG 4211925 7.0993724 40.521652 110-119
TCCCT 4620085 6.599686 96.285324 30-34
CAGTA 3631970 6.477087 43.327335 60-69
ATACA 2599020 6.2737756 31.564892 110-119
GGAGA 4505835 6.106331 91.60629 35-39
GGTGA 4943705 5.8403797 93.21506 45-49
AGTAA 2687310 5.816611 42.974384 110-119
CCCTG 4715030 5.7081127 57.94244 30-34
GTAAT 2888540 5.45022 37.46172 110-119
ACAGT 2769920 4.939747 33.624916 110-119
AGACG 3257220 4.922881 99.43253 40-44
GTAGT 3501425 4.8808856 25.046669 70-79
GTGAC 3704140 4.8802614 83.06796 45-49
GGCCC 4721035 4.843731 58.28713 60-69
GAGAC 3158805 4.774139 129.22557 40-44
CCCCA 3053575 4.729338 99.8792 60-69
ACCAG 2630105 4.43315 97.11377 45-49
GTGTC 3822715 4.39047 25.276106 120-129
AATAC 1802365 4.3507295 31.654331 110-119
ACTCC 2647275 4.337994 97.02285 30-34
GCACA 2526680 4.2588234 33.581413 110-119
CGTGT 3693465 4.242024 24.150768 120-129
TGACC 2860575 4.203162 151.00314 45-49
GACCA 2422790 4.0837126 108.63952 45-49
CCCTT 2797330 3.9959226 59.679344 20-24
TAGTA 2116205 3.9929457 24.724558 80-89
CTCAG 2695220 3.9601986 30.545336 140-149
CACTC 2416075 3.9591348 81.53755 25-29
CCCAG 2834410 3.936292 82.86946 60-69
CCACT 2401545 3.9353251 27.911135 240-247
TGGTG 3800570 3.9139996 26.423483 25-29
AGTAG 2423250 3.8749726 28.704008 70-79
TGAGG 3251670 3.8414488 69.877304 35-39
CAGGG 3356495 3.7478006 34.974686 50-59
CTCCC 2758360 3.724141 59.8464 30-34
GACGG 3302695 3.6877284 112.051216 40-44
AGGAG 2699545 3.6584382 91.60027 35-39
CTGAG 2744920 3.6164742 98.058395 35-39
ACGGT 2724615 3.589722 93.79464 40-44
CTTGG 3101820 3.5625067 34.520733 20-24
ATGGT 2546990 3.5504308 25.128124 190-199
GCCCC 3080475 3.5247445 73.67328 60-69
TCTTG 2592120 3.5128489 19.366901 170-179
TGTCT 2573040 3.4869916 20.99015 180-189
AGATA 1579995 3.419857 18.437119 150-159
AGGCT 2568400 3.3839061 41.740623 30-34
GAGGA 2495430 3.3818197 128.42076 35-39
CCAGG 2706060 3.3697288 40.364563 50-59
ATGCA 1883940 3.3597314 81.69182 25-29
TGTCC 2585180 3.3112884 19.482168 120-129
TTCCC 2316725 3.3093889 57.288933 50-59
GGGTT 3207110 3.3028276 44.897366 50-59
CCTGG 3023360 3.281942 32.377914 50-59
CTTCA 1885800 3.2695177 16.466908 200-209
GCCCT 2671560 3.2342458 31.041983 20-24
CTCTC 2230600 3.1863613 14.196179 100-109
TCAGG 2418295 3.1861408 20.273521 140-149
TGCAC 2112545 3.1040504 105.00607 25-29
CCGTG 2838290 3.0810432 24.216919 120-129
GATGG 2603555 3.0757806 30.554096 15-19
ACAGA 1497460 3.0634394 14.355249 140-149
CCAGT 2081160 3.0579352 42.16414 60-69
GGATG 2587630 3.056967 58.39642 20-24
GGTTC 2656120 3.0506108 50.58384 50-59
TCGCA 2075315 3.0493467 24.740314 100-109
CGCAC 2144720 2.9784837 25.246122 100-109
TACAC 1489250 2.9619124 22.887812 110-119
TCTCA 1706730 2.9590538 20.73 130-139
GACTC 1997510 2.9350247 21.291725 190-199
TGGCC 2688440 2.9183767 39.98032 60-69
TGGAG 2456545 2.9021063 29.138752 25-29
AGGGT 2450215 2.8946278 52.510944 50-59
CCTGA 1960970 2.881335 68.118576 30-34
TCTCT 1902290 2.8750684 15.528145 180-189
GTTCC 2240545 2.8698545 57.56527 50-59
GCACT 1937575 2.8469598 74.137665 25-29
TTCTT 1766090 2.8241158 21.480436 170-179
CGGAT 2136560 2.8149505 66.05563 20-24
CAGAT 1567690 2.7957458 15.441148 150-159
GGTTG 2695840 2.7762985 61.272476 15-19
GCCGT 2553795 2.7722158 22.94924 120-129
CGGTG 2816045 2.7410278 68.06223 40-44
TCAAA 1135190 2.7402356 56.662575 70-79
AAAAA 814385 2.7370348 11.665324 220-229
ACCAC 1450960 2.727482 15.260067 230-239
TCACA 1363585 2.7119823 12.249999 130-139
AGTCA 1517965 2.7070684 30.365158 70-79
AGGGA 1956525 2.6514928 9.616561 150-159
AATAA 903920 2.6482809 12.668762 110-119
CGGCC 2546785 2.6129742 11.255777 120-129
GGCTG 2657720 2.5869203 23.081146 30-34
GTTGG 2507065 2.5818894 47.604298 15-19
GATGC 1922835 2.5333645 77.96024 25-29
CTGGC 2326120 2.525068 35.936768 60-69
AGATG 1554840 2.4863148 16.731085 180-189
TGTTC 1801205 2.4409986 12.717256 140-149
GAGAT 1490885 2.3840454 16.187912 180-189
ACTCT 1362020 2.3614106 12.954243 190-199
GTCTC 1829715 2.3436334 12.69291 180-189
GTTCT 1720820 2.3320606 18.370527 160-169
CAGAG 1540920 2.328908 9.233942 140-149
TCATT 1266115 2.3225193 16.537588 150-159
CATTT 1256220 2.3043683 17.679647 150-159
TTCAT 1252340 2.297251 17.12924 140-149
TTGTC 1694710 2.2966762 14.89526 170-179
CTGGA 1741285 2.2941697 14.729779 180-189
TCCAT 1318225 2.2854807 11.355763 70-79
ACAGC 1354070 2.2823403 11.924438 110-119
TCCTC 1579210 2.2558656 14.962046 130-139
GGCGG 2725330 2.2481625 37.790215 20-24
TGTAG 1600730 2.2313716 8.888656 210-219
GCGGA 1991470 2.2236388 79.56415 20-24
ATATA 869630 2.2210176 10.770608 110-119
CACTA 1116725 2.2210116 14.893284 240-247
TAGTC 1425235 2.215682 25.875454 70-79
GTGAA 1362290 2.1784117 16.675804 190-199
ACTAC 1091135 2.1701167 12.569847 230-239
TTCAC 1248185 2.1640487 17.038084 200-209
TTGGT 1774240 2.1560044 38.17738 25-29
AATAT 826560 2.1110177 12.312781 110-119
CTACT 1216610 2.1093054 8.681115 230-239
TGAAT 1117175 2.1079333 14.613228 190-199
GAATC 1176570 2.0982406 15.118652 190-199
ATTTG 1272125 2.0924203 15.268983 150-159
GTCAA 1171960 2.0900192 40.85761 70-79
GAGTC 1585425 2.0888216 12.723681 210-219
TTGGA 1498070 2.0882664 16.778963 170-179
TCTGC 1623395 2.0793636 10.921153 210-219
CAGCC 1496370 2.0780864 10.949925 120-129
CTCTG 1621800 2.0773206 15.071001 180-189
TCTCG 1612375 2.0652483 16.943764 100-109
GATAC 1154535 2.0589442 13.420535 150-159
TTGGG 1990765 2.0501802 74.68712 15-19
GATAT 1086560 2.0501676 9.846605 180-189
ATATG 1075305 2.0289311 9.904172 180-189
CTCGC 1675775 2.0287275 17.04282 100-109
CCTTC 1419155 2.0272307 13.299743 200-209
GTTCA 1303245 2.0260353 15.451852 140-149
TCGGC 1860260 2.019364 8.528301 190-199
ATAGA 907355 1.9639456 9.908094 240-247
AATAG 906295 1.9616513 8.08211 240-247
AATCG 1095565 1.9537799 13.519806 190-199
TACAG 1092530 1.9483675 12.165376 160-169
CACGG 1561430 1.9443752 7.333753 200-209
TACTA 921245 1.9385539 11.071732 230-239
TACCA 971110 1.9314036 12.286135 230-239
ACACA 844160 1.925954 13.4563265 110-119
GCTGT 1657485 1.9036572 9.823887 140-149
TTGCA 1219615 1.8960233 13.244744 150-159
CCTTG 1477375 1.8923305 39.09088 20-24
GGTCA 1436170 1.892176 9.956002 130-139
GTCAC 1286550 1.8903816 9.41364 130-139
GAACT 1059535 1.8895257 10.873366 160-169
GTTGT 1553985 1.8883569 7.704704 210-219
TCCCA 1149680 1.883939 11.592708 240-247
GTCCT 1460020 1.8701009 12.878587 130-139
GCTGA 1407640 1.8545873 35.829594 30-34
AGTCT 1186185 1.8440529 13.321167 210-219
TGGAA 1151245 1.8409337 9.019282 170-179
ATTGT 1117305 1.8377688 8.4093075 170-179
AGTAT 973965 1.8377186 11.829078 220-229
TTTGC 1354945 1.8362259 11.7079 150-159
CATAG 1023285 1.8248792 8.249614 210-219
TGGGG 2083670 1.8185937 44.582256 15-19
TCAGC 1236610 1.8170027 12.006592 140-149
CAGGC 1453950 1.8105353 13.9484215 140-149
CTGTT 1331390 1.8043038 12.466713 140-149
GTCTG 1540905 1.7697624 8.871505 210-219
ATAGT 930580 1.755858 7.158037 220-229
TTGTA 1054985 1.7352632 10.155202 210-219
CTCCA 1055015 1.728815 8.231937 240-247
ATGTA 913595 1.7238098 9.450497 70-79
CAGCT 1169475 1.7183584 9.404652 140-149
ATCTC 988665 1.7141042 7.036272 100-109
AGAAC 828245 1.694388 10.6414175 160-169
TTCAG 1083455 1.684348 9.99164 150-159
GGGGC 2039835 1.682688 41.10573 15-19
CACCA 894670 1.6817805 10.797829 230-239
TCTGG 1456780 1.673143 11.986551 180-189
TGACT 1074550 1.6705042 12.502231 190-199
ACGGC 1338650 1.6669577 12.620458 120-129
GCAGA 1102170 1.6657922 13.42315 150-159
TGCAG 1244230 1.6392921 12.482298 150-159
CCCAC 1044495 1.6177005 10.829206 240-247
GACGT 1226980 1.616565 10.082639 170-179
ATAAT 632855 1.616299 5.5472736 100-109
GAGAA 877950 1.6104852 10.567861 160-169
GGGCG 1947285 1.6063423 49.154266 20-24
GTGGA 1357875 1.6041625 35.954945 25-29
CACTG 1090080 1.6016998 7.1575384 230-239
GGAGG 1599080 1.6010079 25.242357 30-34
AACCA 701075 1.5995051 12.631479 70-79
GGACG 1432415 1.599408 7.9915786 170-179
CCAGA 944095 1.591311 21.640045 60-69
ATAAA 538705 1.5782835 6.3237066 110-119
CAAAG 770560 1.5763785 29.587997 70-79
AACTG 878330 1.566373 10.555926 160-169
ACTGA 875990 1.5622 7.441004 180-189
AGCTC 1060660 1.558472 9.990401 140-149
TGGTT 1280155 1.5556067 16.377178 10-14
CAAAA 557735 1.5444139 19.65431 70-79
CTCTT 1016070 1.5356601 8.948449 200-209
CGACT 1044705 1.5350287 9.752762 190-199
ACACG 892605 1.5045223 9.579938 110-119
TCGAC 1023015 1.5031586 10.173535 190-199
TCAGA 841765 1.5011648 9.025091 130-139
ACGGA 987560 1.4925735 9.764133 200-209
GTCCC 1228830 1.4876468 15.461059 50-59
TCCAC 905160 1.483253 10.642353 240-247
GCTCT 1151430 1.4748361 9.28153 130-139
ATCGG 1117835 1.4727647 9.383552 190-199
CCTCG 1214435 1.4702201 9.000199 130-139
CTGGT 1267850 1.456153 6.921977 160-169
TGGAC 1101505 1.4512497 10.9456835 170-179
GAGGG 1447920 1.4496657 9.449043 200-209
ATGGG 1223290 1.445167 50.046406 15-19
TAGTT 874210 1.4379205 8.791233 220-229
CCATC 875430 1.4345356 11.272213 240-247
AAATA 489560 1.4342997 5.5126095 6
GGCCG 1556665 1.4320918 11.946099 120-129
AGCCG 1145595 1.4265554 10.50782 120-129
AATTG 754245 1.4231415 9.379321 170-179
CTCGA 966490 1.4201039 9.250301 190-199
CCACC 915865 1.4184799 8.085497 230-239
CTTGA 904335 1.4058865 9.497675 200-209
CGTAG 1051065 1.3847942 8.0151005 210-219
ACTAT 652075 1.3721459 6.8676915 230-239
TATGG 983265 1.3706434 7.1836143 180-189
GGGAG 1366145 1.3677921 6.968306 150-159
ATCCA 687315 1.3669746 17.058968 240-247
CAATA 562410 1.3576015 7.2515154 100-109
GAATT 718800 1.3562624 10.632554 170-179
GTGCT 1157665 1.3296032 7.294268 220-229
ACCAT 667055 1.3266802 9.94979 45-49
GGCTC 1218895 1.3231444 8.321213 130-139
AAGTA 609665 1.3196034 17.830198 70-79
TATAC 625555 1.3163406 6.606018 110-119
CATGT 842730 1.3101149 9.1421995 70-79
TTCCA 749495 1.2994417 7.3976088 230-239
ACGTG 985985 1.2990503 9.700022 170-179
ACTGT 834855 1.2978723 5.7165833 230-239
AAAGT 599055 1.2966384 22.491325 70-79
TGATA 685530 1.2934872 7.148222 180-189
ACTCG 877785 1.289766 9.588482 190-199
CAGAC 755890 1.2740836 10.837401 60-69
TGTGC 1101215 1.264769 6.078178 220-229
ACTTC 727835 1.2618884 5.4941993 220-229
GAGGC 1128335 1.2598779 22.148802 30-34
CTGAT 807380 1.2551595 6.3782673 180-189
GCTCA 852045 1.2519451 9.649982 140-149
CCATG 847335 1.2450247 9.164177 70-79
TCACG 846340 1.2435627 8.352564 200-209
GTGTT 1022260 1.2422204 7.3583364 160-169
GGAAT 776800 1.2421659 8.644573 170-179
TATCA 590275 1.2421018 5.5169888 70-79
TTGAG 883775 1.231957 8.615468 200-209
CCTCA 742720 1.2170686 5.9656243 130-139
GGAGT 1018415 1.2031322 6.9572096 210-219
GAACC 711265 1.1988664 9.257236 70-79
AGTTC 767095 1.1925322 5.88089 160-169
CTGAA 668330 1.1918688 13.024327 35-39
AGAGA 649600 1.1916069 12.480987 35-39
GGTGG 1359660 1.1866895 36.432022 25-29
GGGCC 1284825 1.182006 27.815838 15-19
GTCCA 803450 1.1805425 10.456807 70-79
GCGTA 888410 1.1704938 8.9165535 210-219
GTCTA 738510 1.1480936 7.562286 180-189
GCATA 643430 1.1474633 7.996501 210-219
ATCAT 543860 1.1444317 5.474355 90-99
TGGGC 1175540 1.1442245 29.84292 15-19
ACTGG 866830 1.1420618 6.925907 160-169
AGATC 640190 1.1416852 5.8681154 130-139
CTGCG 1046585 1.1360972 6.3606997 210-219
TGCCC 909890 1.1015315 8.54143 60-69
ACAAT 455110 1.0985901 8.544907 100-109
TAGAT 579075 1.0926234 7.958561 240-247
GAAGA 593620 1.0889188 17.33966 35-39
TCTAC 624630 1.0829562 6.971081 180-189
AGAGC 714695 1.0801722 6.4508066 140-149
TATGT 655615 1.0783706 7.082149 220-229
CCAAT 540570 1.0751191 11.229985 240-247
AGGTT 770390 1.0739015 18.838554 10-14
AAAAG 430500 1.0689119 12.92304 70-79
CGGCT 983270 1.067367 7.337191 130-139
CCCAA 567090 1.0660031 9.699414 240-247
CTCGG 980730 1.0646098 7.4515624 130-139
GCGGT 1093020 1.0639029 6.985891 130-139
ATAAC 434180 1.0480672 7.0254564 80-89
TATAA 403495 1.0305182 7.388252 240-247
AAGAG 553830 1.0159291 12.412132 35-39
TCCAG 690120 1.014022 5.509634 240-247
TCTGT 748045 1.0137528 5.7194514 210-219
TGGTC 879205 1.0097858 7.592015 50-59
ATATC 476815 1.0033505 5.468402 70-79
TACTG 643555 1.0004759 5.174469 180-189
CGGTC 906310 0.9838248 7.1824613 130-139
GTGGT 955245 0.9837547 6.707082 50-59
TGTAA 520345 0.981809 6.923356 230-239
GCCAC 702920 0.9761814 5.0885773 80-89
GTCAG 734430 0.96762276 5.416167 130-139
ATACG 541805 0.96623 5.0231347 120-129
ACTAA 398605 0.9621928 11.248109 240-247
GCTTC 750540 0.96134675 5.336238 150-159
ACCCA 509605 0.957944 11.32991 240-247
AGCTT 615910 0.9574987 5.5980372 150-159
CATCA 478600 0.95186925 5.5197573 230-239
GAGTT 682530 0.95142716 5.2454977 160-169
CGGAG 849875 0.94895476 6.487628 210-219
TAGAC 531025 0.9470054 5.0507526 110-119
CTTCC 645640 0.92228204 5.2915907 230-239
CTACC 560045 0.91772556 6.810327 230-239
CGATG 692855 0.9128471 20.89724 15-19
TGAAG 559360 0.89446187 9.299783 35-39
ACCGT 603140 0.8862188 12.238202 45-49
CCAGC 636590 0.8840655 5.7777658 80-89
TGCAT 568565 0.8838958 6.5387383 210-219
AATCC 440905 0.87689906 10.64727 240-247
ACGTC 595100 0.87440526 6.9979963 70-79
GAGCT 653090 0.860456 5.426339 140-149
TGAAC 478355 0.85307616 5.1909094 190-199
TGCGT 740515 0.8504974 6.854093 210-219
ATCAA 347510 0.8388545 12.346952 70-79
AGCCA 497635 0.8387843 7.892295 80-89
AGCTG 628650 0.828256 6.606601 30-34
AAAGA 328075 0.81459516 5.1323657 70-79
AGACC 481975 0.8123886 5.5225754 240-247
TAACC 398385 0.7923327 6.506996 80-89
GGAAG 577755 0.78297675 8.792745 30-34
CTAAT 365580 0.7692813 8.964034 240-247
CCTTT 507795 0.76746744 5.812084 50-59
AAAGG 413785 0.7590348 7.3867927 10-14
CAATC 375955 0.74772245 10.536744 240-247
TCGAA 410850 0.7326909 12.807129 70-79
TGAGA 456735 0.73035616 5.8536916 240-247
ATACC 359975 0.71594054 6.1820483 240-247
TAGGT 513210 0.7154 5.891824 10-14
AGGAT 440675 0.7046749 7.8197265 10-14
TGGAT 491160 0.6846629 6.9206643 10-14
GTCGA 516760 0.68083924 9.149542 70-79
AATGG 419450 0.67073447 5.382282 10-14
GACCG 536170 0.6676672 12.566403 45-49
CGAAC 393610 0.6634458 9.228516 70-79
ACGAT 367975 0.6562296 8.092457 10-14
AAGGT 409680 0.6551114 6.8720727 10-14
CGTCC 538615 0.65205836 5.537689 70-79
GGTCC 600440 0.6517944 8.436598 50-59
CATCC 390260 0.63950497 7.551234 240-247
AAAGC 306780 0.6275973 8.663185 70-79
CTTTG 441740 0.5986474 5.0117307 50-59
ATAGG 353205 0.56480336 5.15445 10-14
GAAGC 363435 0.5492866 8.32138 30-34
TCAAG 290630 0.5182961 5.275622 70-79
AAGCT 289445 0.5161828 13.593668 30-34
TTGCC 401660 0.51447564 5.799404 60-69
GACGA 332330 0.5022753 6.320108 40-44
CGGTT 435660 0.5003649 10.216719 10-14
TAAAG 227495 0.49240685 5.165619 9
GGGAT 398720 0.47103867 5.5860257 10-14
AAGCA 227310 0.46502104 5.43151 70-79
TCGAT 268175 0.41690704 6.0590744 10-14
GCGAT 230725 0.30398372 5.089786 10-14