FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_13_SL254623.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_13_SL254623.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4468018
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 4728595 7.307739 50.575577 110-119
CACAG 6708075 6.795094 35.409317 110-119
TCCCT 6842685 6.236033 87.82481 30-34
AAAAA 3265965 6.1793013 38.324215 230-239
CAGTA 5293520 5.779097 37.424614 60-69
GGAGA 6801200 5.678852 84.70424 35-39
ATACA 3919220 5.619952 27.036924 110-119
GGTGA 7355540 5.5507426 86.16346 45-49
CCCTG 6917640 5.310809 52.303745 30-34
AGTAA 3961815 5.1578174 37.60111 110-119
GTAAT 4236660 4.984905 33.443657 110-119
ACAGT 4523960 4.9389453 30.134031 110-119
GGCCC 7292985 4.716599 53.24685 60-69
GTAGT 5186480 4.6460977 23.379717 70-79
GAGAC 5017200 4.614213 115.04197 40-44
ACTCC 4505745 4.5434623 90.96908 30-34
CCCCA 4820690 4.510365 89.10376 60-69
GTGAC 5409545 4.4963336 76.72749 45-49
AGACG 4878350 4.4865165 88.78557 40-44
GTGTC 5636985 4.2345395 24.105259 120-129
CCCTT 4554225 4.150461 63.50508 20-24
GCACA 4068290 4.121065 29.465813 110-119
CGTGT 5440475 4.0869193 23.142345 120-129
ACCAG 4033830 4.0861583 85.56817 45-49
TGGTG 5867525 4.0017776 29.196466 25-29
CACTC 3939765 3.9727447 77.913 25-29
TGACC 4217850 3.8614478 138.84647 45-49
AATAC 2664635 3.8209448 27.004698 110-119
TAGTA 3230240 3.8007388 24.524857 80-89
CCCAG 4447320 3.7778053 75.07738 60-69
TGAGG 4976365 3.7553353 66.1373 35-39
CTCAG 4093535 3.7476373 27.417852 140-149
CCACT 3661345 3.691994 24.952745 240-247
GACCA 3626115 3.673154 95.90162 45-49
CTTGG 4840335 3.6360908 38.712563 20-24
AGTAG 3609425 3.5776029 24.89408 70-79
CTCCC 4199715 3.5512712 53.405365 30-34
TGTCT 3953880 3.5258348 20.146303 180-189
CAGGG 4995200 3.4976113 31.166986 50-59
CTGAG 4189585 3.4823208 90.71473 35-39
TCTTG 3851920 3.4349124 18.87993 170-179
GACGG 4897080 3.4289083 103.63468 40-44
AGGAG 4106400 3.4287531 84.28834 35-39
CTTCA 3152845 3.4264216 16.29279 200-209
GCCCC 4758195 3.3894334 67.666176 60-69
ATGGT 3766075 3.3736854 23.632761 190-199
ACGGT 3995680 3.3211503 87.68076 40-44
AGGCT 3940975 3.2756798 42.429207 30-34
AGATA 2479590 3.2281346 16.482315 150-159
CCTGG 4630105 3.2272472 30.580124 50-59
GGGTT 4712345 3.2139204 41.758484 50-59
GCCCT 4176680 3.2065198 33.077824 20-24
TGTCC 3857270 3.1915424 18.371063 120-129
ATGCA 2918420 3.1861277 75.97607 25-29
TTCCC 3488715 3.179416 52.894955 50-59
AGTCA 2908925 3.1757617 26.526299 70-79
GATGG 4150380 3.1320188 32.61838 15-19
CTCTC 3426735 3.122931 13.093492 100-109
GAGGA 3720920 3.1068861 116.56225 35-39
CCAGT 3383450 3.0975537 37.916138 60-69
TCAGG 3710155 3.0838256 19.033863 140-149
CCAGG 3996275 3.082019 35.974876 45-49
GGATG 4073075 3.073682 58.07367 20-24
TGCAC 3283520 3.0060673 100.729576 25-29
CCGTG 4280675 2.98369 22.55638 120-129
TCAAA 2064520 2.9604115 48.438396 70-79
TCACA 2458035 2.95573 11.316982 140-149
GGTTC 3921095 2.945552 46.486668 50-59
TGGAG 3890420 2.9358442 31.97676 25-29
TCTCA 2611870 2.8385055 19.157621 130-139
TCTCT 2874760 2.823588 14.910138 180-189
TTCTT 2651550 2.806838 21.028835 170-179
GCCGT 4016340 2.7994447 21.524755 120-129
GGTTG 4093725 2.7920084 61.81833 15-19
TCGCA 3049300 2.7916386 22.670572 100-109
CGGAT 3334265 2.7713919 64.597336 20-24
ACAGA 2290450 2.7667806 12.606406 140-149
TGGCC 3942665 2.7480924 36.558746 60-69
GACTC 2999320 2.7458823 18.98452 190-199
CAGAT 2514095 2.7447143 14.292313 150-159
GCACT 2997765 2.7444584 71.56297 25-29
TACAC 2276830 2.737835 19.777716 110-119
AGGGT 3568650 2.6930254 47.56114 50-59
CGCAC 3163730 2.6874514 22.660435 100-109
GTTCC 3214775 2.659936 52.30058 50-59
CGGTG 4156920 2.6305819 65.67919 40-44
GGCTG 4111120 2.6015987 24.667055 30-34
AGGGA 3085705 2.5764956 8.936006 150-159
GTTGG 3777520 2.5763497 48.12082 15-19
ACCAC 2306235 2.5731342 13.366028 230-239
CCTGA 2802575 2.5657613 60.430115 30-34
GATGC 2958175 2.458791 74.77815 25-29
CGGCC 3791800 2.4522743 10.045604 120-129
AATAA 1406270 2.4046845 11.275836 110-119
TGTTC 2696340 2.4044356 12.635262 140-149
CTGGC 3437045 2.395668 32.571842 60-69
GTCTC 2893345 2.3939815 11.903242 180-189
TCCTC 2604250 2.373365 14.024125 130-139
AGATG 2392075 2.3709857 15.672851 180-189
GAGAT 2353410 2.3326614 15.208488 180-189
ACTCT 2120705 2.3047216 12.025483 200-209
TTGTC 2578310 2.2991834 14.722743 170-179
GTCAA 2096685 2.2890146 35.94989 70-79
GGCGG 4272040 2.2773843 37.317417 20-24
GTTCT 2545635 2.2700458 17.912994 160-169
TTGGT 2783025 2.2531714 42.189087 25-29
TCTGC 2698690 2.232922 11.051746 210-219
CTGGA 2675720 2.2240186 14.043275 180-189
CATTT 1894210 2.2186265 16.228033 150-159
CAGAG 2410015 2.2164402 8.598437 140-149
TCATT 1881000 2.2031538 15.070995 150-159
GCGGA 3144675 2.2018838 76.85522 20-24
TTCAT 1874590 2.1956458 16.426529 140-149
TTCAC 2009940 2.1843452 16.136133 200-209
TCTCG 2604030 2.1545997 15.954901 100-109
TGTAG 2397090 2.147336 8.39594 210-219
GATAT 1816880 2.1377628 9.265314 180-189
TCCAT 1953470 2.1229753 11.608197 70-79
TTGGG 3084595 2.10376 76.027 15-19
CTCTG 2535005 2.0974874 14.291698 180-189
ACAGC 2066135 2.0929377 10.89836 110-119
TTGGA 2316910 2.07551 15.856567 170-179
GTGAA 2093510 2.0750527 15.638855 190-199
ATTTG 1939370 2.0623171 14.598435 150-159
TGAAT 1751945 2.0613594 13.693292 190-199
CCTTG 2485840 2.0568078 43.140614 20-24
GAGTC 2467295 2.0507789 11.792713 210-219
GAACT 1876740 2.0488942 12.362715 160-169
GTCTG 2725145 2.0471463 8.7872925 210-219
TAGTC 2070475 2.0428991 22.969011 70-79
GTCAC 2230815 2.0423143 8.905298 130-139
CCTTC 2234825 2.0366921 13.035994 200-209
GTTCA 2045145 2.0179067 14.550977 140-149
GATAC 1835630 2.004013 12.227609 150-159
CACTA 1663360 2.0001519 12.846421 240-247
GAATC 1827905 1.9955795 13.83716 190-199
ATATA 1289420 1.9927155 9.377808 110-119
CTCCA 1975085 1.9916184 7.720826 240-247
CAGCC 2315450 1.9668742 10.070974 120-129
TCGGC 2799680 1.9514158 8.268132 190-199
AATAT 1259450 1.9463987 11.085953 110-119
TCAGC 2121020 1.9417969 10.506369 140-149
CTCGC 2497730 1.9175568 15.932295 100-109
CTACT 1763215 1.9162116 7.6637864 230-239
TTCAG 1927350 1.9016802 9.824324 150-159
ACTAC 1581080 1.9012119 11.039297 230-239
ATATG 1611065 1.8955983 8.999216 180-189
GTTGT 2332560 1.8884695 7.542359 210-219
CACCA 1683025 1.8778005 9.705816 230-239
TGGGG 3259030 1.8724371 45.32298 15-19
CCAGA 1847635 1.871603 21.212496 60-69
TTTGC 2091360 1.8649501 11.485264 150-159
ATAGA 1419550 1.8480872 9.047113 240-247
TTGCA 1865100 1.8402596 12.901226 150-159
AGTCT 1863635 1.8388139 12.306046 210-219
GCTGT 2447470 1.838555 9.61965 140-149
ACACA 1372480 1.826086 11.5586 110-119
AATAG 1400775 1.8236444 7.606353 240-247
AATCG 1669480 1.822622 12.547936 190-199
GCTGA 2192640 1.8224897 36.799377 30-34
TCCCA 1801420 1.8164996 10.594188 240-247
ATCTC 1667710 1.8124197 6.472135 100-109
GTCCT 2187440 1.8099089 12.161878 130-139
TACAG 1654930 1.8067373 10.51244 160-169
CACGG 2330735 1.7975163 6.8957143 200-209
ATTGT 1683905 1.7906567 8.089792 170-179
GGTCA 2119210 1.761456 9.264792 130-139
TGGAA 1775680 1.7600249 8.657992 25-29
CTGTT 1965340 1.752573 12.153491 140-149
CAGGC 2244725 1.7311835 12.847549 140-149
AGTAT 1470530 1.7302431 10.935582 220-229
TACCA 1408020 1.6931114 10.477665 230-239
TACTA 1305760 1.6922243 9.776811 230-239
GTGGA 2241420 1.6914524 39.16185 25-29
GGGGC 3168825 1.6892709 41.207005 15-19
TCTGG 2235355 1.679213 11.861104 180-189
TTGTA 1571710 1.6713493 9.507437 210-219
ATCGG 1993500 1.6569675 9.059886 190-199
CATAG 1516360 1.6554562 7.0654087 210-219
GGAGG 2591435 1.647395 26.312113 30-34
CCCAC 1756345 1.6432828 9.697905 240-247
ATGTA 1394220 1.6404558 9.965368 70-79
ACACG 1603910 1.6247164 8.416802 110-119
TGCAG 1942100 1.6142447 12.043221 150-159
CAGCT 1762720 1.6137729 8.50781 150-159
ATAGT 1365370 1.6065106 6.4657593 220-229
GGGCG 3010690 1.6049707 48.457092 20-24
CTCTT 1622970 1.5940803 8.781142 200-209
TGGTT 1950280 1.5789708 17.17829 10-14
GCAGA 1707695 1.5705312 12.317077 150-159
CTTGA 1580185 1.559139 9.342959 200-209
TGACT 1577085 1.5560803 11.325429 190-199
ATGGG 2060135 1.5546484 53.423557 15-19
GACGT 1848150 1.536155 9.348303 170-179
ACGGC 1973160 1.5217463 11.093824 120-129
AGAAC 1257745 1.5193105 9.478756 160-169
CCACC 1621985 1.517572 7.1098 230-239
CAAAG 1254815 1.5157712 24.36591 70-79
GTCCC 1969100 1.5117171 15.959574 50-59
CACTG 1648290 1.5090119 6.095798 230-239
ATAAT 970765 1.5002548 5.126342 110-119
GGACG 2140585 1.4988258 7.340288 170-179
ACTGA 1365060 1.4902774 6.8142567 180-189
GCTCT 1795050 1.4852417 8.931253 130-139
CTGGT 1971620 1.4810934 6.666967 160-169
CTGAA 1348035 1.4716907 12.871497 35-39
CGACT 1600115 1.4649078 8.815716 190-199
AGCTC 1591525 1.4570436 8.774682 140-149
AACTG 1332395 1.4546161 9.558983 160-169
GAGAA 1326215 1.4544759 9.427432 160-169
CAAAA 916020 1.453371 17.37252 70-79
GAGGG 2272500 1.4446453 8.742028 200-209
TCCAG 1567710 1.4352407 5.1668634 240-247
AACCA 1078320 1.4347057 10.610616 70-79
GGAAT 1446205 1.4334549 8.215895 170-179
ATAAA 834255 1.426554 5.6797304 110-119
GAAGA 1300715 1.4265099 16.646559 35-39
TCGAC 1552280 1.4211146 9.212538 190-199
ACGGA 1545065 1.4209639 8.995802 200-209
TGGAC 1704790 1.4169961 10.170927 170-179
CCTCG 1833085 1.4072956 8.497165 130-139
GAGGC 2005670 1.404359 22.726017 30-34
CGTAG 1676745 1.3936855 7.8147874 210-219
TCAGA 1276340 1.3934191 7.791944 130-139
TCCAC 1376470 1.3879924 9.541914 240-247
CAGAC 1360720 1.3783717 10.968596 60-69
AGATC 1257030 1.3723379 6.4302535 140-149
GGCCG 2281815 1.3398086 10.996879 120-129
AGCCG 1734445 1.3376439 9.827203 120-129
CCATC 1323560 1.3346394 9.935738 240-247
CTCGA 1453240 1.3304433 8.395709 190-199
ATCCA 1105450 1.329278 14.864546 240-247
AATTG 1129000 1.3283949 8.574601 170-179
GGGAG 2088935 1.3279519 6.4430013 150-159
TAGTT 1248050 1.3271705 7.8224277 220-229
GGCTC 1898905 1.3235632 7.840793 130-139
GGTGG 2284660 1.3126245 39.879883 25-29
AAGAG 1187620 1.3024771 11.616705 35-39
GTGTT 1597620 1.293453 7.1882215 160-169
TATGG 1436150 1.2865167 6.592389 180-189
AGAGC 1382655 1.2715988 7.136928 140-149
GAATT 1080550 1.2713882 9.501865 170-179
CATGT 1270065 1.2531494 9.552512 70-79
GGGCC 2133775 1.2528843 30.4671 15-19
GTGCT 1666355 1.2517766 7.1504784 220-229
CAATA 872025 1.2504375 6.7538414 100-109
CCTCA 1234650 1.2449852 5.4466553 130-139
TGGGC 1967030 1.2447759 33.26534 15-19
ACTAT 960210 1.2444022 6.557619 230-239
TTCCA 1138635 1.2374359 7.1700196 230-239
ACCAT 1020365 1.2269652 9.630612 45-49
ATATC 946750 1.2269585 5.547901 70-79
ACTCG 1326965 1.2148384 8.644614 190-199
ACTTC 1115450 1.2122393 5.0082183 220-229
AGAGA 1103725 1.2104685 11.782184 35-39
ACGTG 1454695 1.209121 8.867113 170-179
GCGTA 1438815 1.1959217 8.560867 210-219
GGAGT 1580170 1.1924505 6.4338164 210-219
GTCCA 1296075 1.1865585 10.715804 70-79
TGATA 1007070 1.1849306 6.328212 180-189
CCATG 1289160 1.1802279 9.533048 70-79
TGTGC 1569890 1.1793116 5.855672 220-229
TATAC 909455 1.1786253 5.910824 110-119
GCTCA 1286740 1.1780124 8.542644 140-149
TTGAG 1307250 1.171047 8.194353 200-209
AAGTA 895460 1.1657836 15.05958 70-79
TATCA 890375 1.1538982 5.429806 70-79
AAAGT 882390 1.148768 18.376823 70-79
CTGAT 1161875 1.1464003 5.674383 180-189
TAGAT 972230 1.1439375 7.549398 240-247
CTTCT 1152630 1.1321126 5.142262 210-219
AGTTC 1141580 1.1263757 5.430744 160-169
CTGCG 1612060 1.1236281 6.045959 210-219
TGAAC 1020560 1.1141763 5.2774506 190-199
CCCAA 994990 1.1101395 8.293099 240-247
GAACC 1089860 1.103998 8.015443 70-79
AGGTT 1227150 1.0992925 18.882444 10-14
TGCCC 1428280 1.0965188 9.198252 60-69
GTCTA 1111180 1.0963806 7.252232 180-189
ACCCA 976410 1.0894094 10.267081 240-247
ACTGG 1310595 1.0893471 6.4890523 160-169
AAAAG 752995 1.0846816 11.488657 70-79
TCACG 1164745 1.0663257 8.015784 200-209
TATGT 997350 1.0605773 6.904267 220-229
GCGGT 1665250 1.0538033 6.6986394 130-139
GGAAG 1257835 1.0502646 9.403739 30-34
ACAAT 725090 1.0397404 7.5570774 100-109
GCATA 948860 1.0358993 6.5826497 210-219
GTGGT 1517310 1.034838 7.305703 50-59
CGGCT 1484445 1.0346787 7.1298795 130-139
CCAAT 854160 1.0271075 9.778584 240-247
TCTAC 937830 1.0192068 6.7618275 180-189
CTCGG 1459505 1.0172952 7.096994 130-139
ACGTC 1108920 1.015218 7.0447702 70-79
TGGTC 1350250 1.0143164 8.192796 50-59
CCAGC 1184900 1.0065211 5.455823 80-89
GTATG 1119440 1.0028049 5.419001 220-229
TCCCC 1168770 0.98830974 5.6155424 90-99
CTTCC 1075080 0.9797666 5.019954 230-239
TTCTG 1087935 0.97015554 5.113328 210-219
TATAA 623390 0.96340907 6.5747023 240-247
ATAAC 665055 0.9536535 6.0082192 80-89
CGATG 1140520 0.9479834 22.031223 15-19
CATCA 786385 0.94560975 5.0557303 230-239
CGGTC 1347700 0.9393657 6.681707 130-139
CGGAG 1334295 0.934266 5.9032545 210-219
GCTTC 1125305 0.93108815 5.227692 150-159
GAGTT 1033185 0.9255368 5.0177784 160-169
TGTAA 786490 0.9253936 5.922722 230-239
TGAAG 925895 0.91773194 9.34556 35-39
AGCTT 927775 0.9154183 5.497429 150-159
ACCGT 983840 0.90070707 12.624545 45-49
GACCG 1137140 0.8769885 12.689755 45-49
ACTAA 609440 0.87390447 9.890707 240-247
AATCC 725635 0.8725592 9.4836235 240-247
AGCTG 1010780 0.8401454 6.8115067 30-34
TGCGT 1115575 0.838027 6.7445655 210-219
TGCAT 847155 0.83587205 5.489178 210-219
CTACC 827380 0.834306 6.087652 230-239
AAAGG 757290 0.83052903 7.413146 10-14
CCTTT 845080 0.8300371 6.2277555 50-59
GAGCT 995075 0.8270915 5.11566 140-149
ATCAA 574525 0.8238382 11.345465 70-79
AGCCA 757440 0.76726574 6.463386 80-89
TGAGA 769980 0.7631915 5.3190384 240-247
TAGGT 816180 0.7311417 5.9664516 10-14
CAATC 589825 0.70925087 9.318232 240-247
TGGAT 790805 0.70841056 7.0141363 10-14
AGGAT 713725 0.7074325 8.10824 10-14
TAACC 586390 0.7051203 5.6998396 80-89
CTAAT 542565 0.7031473 8.128363 240-247
GGTCC 997605 0.6953446 8.955534 50-59
AAGGT 686230 0.6801799 6.619306 10-14
TCGAA 620305 0.6772058 11.547932 70-79
ATACC 553765 0.6658895 5.4002266 240-247
CTTTG 737510 0.6576674 5.503893 50-59
GTCGA 783755 0.6514455 8.139348 70-79
AATGG 641400 0.63574517 5.4713364 10-14
ACGAT 582300 0.6357147 8.40557 10-14
CATCC 605250 0.6103165 6.4152904 240-247
CGAAC 599925 0.60770744 7.8847375 70-79
AAAGC 497155 0.60054517 8.030886 70-79
TTGCC 704640 0.58302593 6.234066 60-69
CGTCC 756980 0.5811485 5.6913657 70-79
GAAGC 628055 0.577609 8.4567375 30-34
ATAGG 562720 0.5577588 5.0596337 10-14
AAGCT 484420 0.52885604 13.403674 30-34
TCAAG 478055 0.52190715 5.233141 70-79
CGGTT 668610 0.50226414 10.36081 10-14
GGGAT 663560 0.5007451 6.477498 10-14
TGGGT 706745 0.48201525 5.0819964 10-14
GACGA 523300 0.48126805 5.672803 40-44
AAGCA 376035 0.45423663 5.009776 70-79
TCGAT 436645 0.43082947 6.822906 10-14
GCGAT 382085 0.3175834 5.202073 10-14