FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_5_SL254632.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_5_SL254632.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 28312816
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 33143395 8.302413 56.37635 110-119
CACAG 42415410 7.18074 41.03529 110-119
CAGTA 36160495 6.397124 41.513203 60-69
TCCCT 44817450 6.3649826 89.68747 30-34
ATACA 26124195 6.2624664 29.393564 110-119
GGAGA 45840685 6.170173 92.26574 35-39
AGTAA 27378720 5.8521576 42.49074 110-119
GGTGA 49591815 5.773095 93.65036 45-49
CCCTG 45036575 5.457715 53.290836 30-34
GTAAT 29460805 5.446279 36.268883 110-119
GGCCC 50090285 5.1795793 59.263336 60-69
AGACG 33607290 5.073169 102.00349 40-44
ACAGT 28528035 5.0468717 34.100086 110-119
CCCCA 31174605 4.8988447 104.643074 60-69
GTAGT 35349885 4.8227105 24.451878 70-79
GTGAC 36814520 4.806373 84.30817 45-49
GAGAC 31591555 4.768885 133.50981 40-44
GTGTC 38984640 4.4019423 24.50297 120-129
ACCAG 25952330 4.393614 96.31473 45-49
CCCTT 30470450 4.327419 68.1313 20-24
AATAC 18023380 4.320547 29.518673 110-119
TGACC 29032815 4.250961 153.64102 45-49
CGTGT 37362780 4.21881 23.322582 120-129
TAGTA 22595070 4.1770434 27.994247 80-89
GCACA 24590825 4.1631174 35.21868 110-119
GACCA 24277945 4.1101475 107.92631 45-49
TGGTG 39621175 3.989133 30.157644 25-29
ACTCC 24191365 3.9724457 89.908615 30-34
CCCAG 28303360 3.9658022 85.88359 60-69
AGTAG 24636480 3.8862383 27.483982 70-79
CTCAG 26492905 3.8790698 30.014345 140-149
TGAGG 32482265 3.7813334 70.416245 35-39
CCACT 22862420 3.754221 27.131443 240-247
CTTGG 33239260 3.7532034 39.262794 20-24
GACGG 33489505 3.730805 112.87728 40-44
GCCCC 31748400 3.6818197 76.828 60-69
CTGAG 27973520 3.6521232 99.17629 35-39
CACTC 22238620 3.6517868 74.52046 25-29
AGGAG 27065235 3.6429906 91.86348 35-39
CAGGG 32666570 3.6391282 34.590942 50-59
CTCCC 26419110 3.5905697 56.47337 30-34
ACGGT 27411435 3.5787396 92.6947 40-44
TGTCT 26810620 3.5478249 21.868221 180-189
AGGCT 27053015 3.531946 48.016697 30-34
ATGGT 25882505 3.5310962 26.028374 190-199
GCCCT 28793990 3.489373 36.441322 20-24
TCTTG 26363315 3.4886334 20.057775 170-179
AGATA 16100455 3.441446 18.86813 150-159
GAGGA 25485225 3.4303207 129.78093 35-39
TGTCC 26690755 3.379958 18.725574 120-129
CCAGG 26683365 3.3337576 40.228638 50-59
GATGG 28321310 3.2969477 35.222458 15-19
CTCTC 22810560 3.23956 13.642968 100-109
CTTCA 18821700 3.2296934 17.565023 200-209
TCAGG 24601530 3.2118883 20.248575 140-149
CCGTG 29682360 3.2073352 23.926914 120-129
TTCCC 22437960 3.1866434 55.876633 50-59
CCTGG 29114980 3.1460268 31.831413 50-59
ACAGA 15203665 3.1098988 13.943056 140-149
GGGTT 30627530 3.083636 42.881004 50-59
ATGCA 17430480 3.0836124 74.90942 25-29
TGGAG 26416400 3.0751927 33.36646 25-29
TCGCA 20964380 3.069587 23.849241 100-109
TCTCT 20503635 3.0428867 17.18778 180-189
CGCAC 21551720 3.0197778 24.012764 100-109
TCTCA 17342030 2.9757907 20.098495 130-139
GGTTC 25987575 2.9343815 48.53655 50-59
TGGCC 27151065 2.9338152 40.09612 60-69
GGATG 25167740 2.9298334 52.437836 20-24
AATAA 10053670 2.9119208 14.027004 110-119
TACAC 14608840 2.89845 21.050589 110-119
GACTC 19730680 2.8889499 18.904802 190-199
CCAGT 19641170 2.8758438 41.339817 60-69
TGCAC 19600610 2.869905 96.57162 25-29
TTCTT 18261770 2.8320618 22.539478 170-179
TCACA 14262900 2.8298137 12.1266165 140-149
GTTCC 22147605 2.8046405 55.54195 50-59
CAGAT 15751625 2.7866073 15.968159 150-159
GCCGT 25715090 2.7786505 22.795858 120-129
CGGTG 28795845 2.774445 67.2674 40-44
AGGGT 23786350 2.7690227 50.930428 50-59
TCAAA 11498985 2.7565253 57.114773 70-79
CCTGA 18609695 2.7248166 62.717064 30-34
CGGCC 26203975 2.709619 11.553678 200-209
ACCAC 14259810 2.7074406 15.37621 230-239
GGCTG 27947290 2.6926875 26.606562 30-34
AGATG 16670635 2.6296802 17.178982 180-189
GCACT 17951615 2.6284611 67.220024 25-29
CGGAT 20030360 2.615093 59.58517 20-24
AGGGA 19029110 2.5613253 9.04073 150-159
GGTTG 25359830 2.5532744 55.38742 15-19
TGTTC 19260885 2.5487754 13.226712 150-159
AGTCA 14310390 2.5316398 29.619623 70-79
GAGAT 15969025 2.5190058 16.958447 180-189
CTGGC 22921080 2.4767427 35.67894 60-69
GTCTC 19289215 2.4426708 13.865914 180-189
TCATT 13619430 2.4421163 18.142374 150-159
TTGTC 18234475 2.4129515 15.02644 170-179
CATTT 13450955 2.411907 19.366058 150-159
ACAGC 14004220 2.3708518 12.791523 120-129
TTCAT 13201815 2.3672333 17.48044 140-149
GTTGG 23413840 2.3573484 43.154816 15-19
GATGC 17928420 2.3406708 71.40121 25-29
CTGGA 17918215 2.3393383 15.962658 180-189
TCCTC 16415700 2.3313608 16.099855 130-139
GTTCT 17611805 2.330554 17.41645 160-169
ACTCT 13550620 2.3252075 12.624272 200-209
CAGAG 15285235 2.307374 8.777861 140-149
AATAT 9192795 2.3027933 13.230306 110-119
TCCAT 13408870 2.300884 13.147783 70-79
ATATA 9130875 2.2872822 10.892282 110-119
TTCAC 13170060 2.2599056 18.119877 200-209
GTGAA 14299225 2.2556062 18.03548 190-199
TTGGT 18934170 2.2340965 43.55506 25-29
CACTA 11139780 2.210175 15.536094 240-247
ATTTG 13760480 2.2000947 16.679783 150-159
TGTAG 16071865 2.192651 7.9216514 70-79
GAATC 12387495 2.1914616 16.15686 190-199
TGAAT 11783435 2.1783476 15.572108 190-199
CTCTG 17169665 2.174264 15.966286 180-189
GAGTC 16554135 2.1612492 13.811176 210-219
TAGTC 14057470 2.1508496 24.54971 70-79
GGCGG 26010530 2.1384096 34.917046 20-24
ACTAC 10758410 2.1345098 12.452801 230-239
TCGGC 19584785 2.1162386 8.814906 190-199
CCTTG 16662985 2.110101 44.586433 20-24
TTGGA 15454825 2.1084695 16.983812 170-179
TCTGC 16646535 2.1080174 11.251518 210-219
AATCG 11821555 2.0913417 14.261124 190-199
CAGCC 14913160 2.089598 11.546246 120-129
GTTCA 13619990 2.0839133 15.777067 140-149
GATAC 11759170 2.0803053 13.345915 150-159
GTCAA 11718980 2.073195 40.40326 70-79
ACACA 9016175 2.0683303 13.766959 110-119
CTCGC 17007430 2.0610297 16.21977 100-109
GCGGA 18454055 2.0558226 73.29417 20-24
CCTTC 14396825 2.0446398 14.321318 200-209
TACAG 11411925 2.0188744 12.557313 160-169
ATATG 10906490 2.016231 8.623761 180-189
AATAG 9428795 2.01539 7.948222 110-119
GCTGA 15401655 2.0107856 41.61046 30-34
CACGG 16072860 2.0081058 7.9667397 200-209
TCTCG 15854805 2.0077577 15.948096 100-109
CTACT 11659245 2.0006583 8.475117 230-239
TTGCA 13050250 1.9967408 14.391764 150-159
TTTGC 14792095 1.9574244 12.639845 150-159
ATAGA 9136950 1.9530082 9.388594 240-247
TGGAA 12314450 1.9425213 9.680114 170-179
GTCCT 15289965 1.9362296 13.891717 130-139
ATTGT 12071990 1.9301302 8.830686 170-179
GCTGT 17071685 1.9276456 10.409666 140-149
GTTGT 16073770 1.8965899 6.9922037 210-219
CAGGC 15175475 1.8959886 14.918241 140-149
TACTA 9129850 1.8928604 10.802998 230-239
AGTAT 10224575 1.8901688 12.3342495 220-229
AGTCT 12333775 1.8871174 13.893134 210-219
GATAT 10147055 1.875838 8.503724 180-189
TACCA 9442725 1.8734729 12.115916 230-239
GGTCA 14323065 1.8699685 9.761892 140-149
TTGGG 18537665 1.8664063 67.19398 15-19
TCCCA 11298355 1.8552945 11.621496 240-247
CTGTT 13867985 1.8351375 12.803076 140-149
CATAG 10216410 1.8073769 7.573988 210-219
GCAGA 11887880 1.7945284 14.431157 150-159
GTCAC 12226530 1.7901983 9.4633045 140-149
TCTGG 15716780 1.7746565 12.994466 180-189
ATAGT 9564435 1.7681316 7.476438 220-229
GTGGA 15175305 1.7665915 40.945545 25-29
AAAAA 5250705 1.75841 6.48201 6
GTCTG 15497030 1.7498436 9.237259 210-219
ATGTA 9453370 1.7475997 11.243866 70-79
ATAAT 6967075 1.7452508 6.0182104 110-119
GAACT 9823725 1.7379073 10.430341 160-169
TGCAG 13142780 1.7158746 13.5279 150-159
ATAAA 5913460 1.7127602 6.897108 110-119
AGAAC 8297020 1.6971494 10.809678 160-169
TTGTA 10567445 1.689576 9.293736 220-229
GGAGG 16955025 1.684196 29.52044 30-34
CACCA 8798800 1.6705852 11.20119 230-239
ACGGC 13353720 1.6683828 12.29762 120-129
TGGGG 19316640 1.6595037 39.874393 15-19
TCAGC 11313860 1.656566 10.449653 150-159
CAAAA 5956690 1.6510302 20.994413 70-79
AACCA 7194040 1.6503288 12.082976 70-79
ATCTC 9495390 1.6293536 7.0093093 100-109
GACGT 12408020 1.6199471 9.376374 170-179
GTCCC 13340280 1.6166292 17.842655 50-59
ACGGA 10699460 1.6151309 10.20749 200-209
CCAGA 9533240 1.6139349 24.247828 60-69
ATGGG 13818960 1.6086963 56.583374 15-19
CCCAC 10215470 1.6052809 10.997139 240-247
CAGCT 10924885 1.5996126 8.740952 150-159
GAGAA 8764405 1.5985299 10.815237 160-169
TTCAG 10433345 1.5963439 9.699227 150-159
CAAAG 7765225 1.5883713 29.454063 70-79
GGACG 14226045 1.584813 7.5099673 170-179
TGACT 10308610 1.5772588 11.0890045 190-199
AACTG 8900150 1.5745186 10.176872 160-169
CGACT 10701940 1.5669694 9.346309 200-209
AAATA 5401610 1.5645094 6.3649955 6
CCTCG 12888755 1.5619118 10.029404 130-139
GGGGC 18893850 1.5533245 37.289417 15-19
TCGAC 10562975 1.546622 9.33418 190-199
CTCTT 10297130 1.5281682 8.68971 200-209
CACTG 10405080 1.5235034 6.1530232 230-239
AGCCG 12044615 1.5048262 11.135201 120-129
GGGCG 18221710 1.4980656 45.33649 20-24
TGGTT 12665825 1.4944768 15.415469 10-14
CTCCA 9095150 1.4935079 7.8246574 240-247
GCTCT 11776845 1.4913493 9.396577 130-139
AATTG 8053820 1.4888716 9.817608 170-179
ACTGA 8360160 1.4789894 6.977112 180-189
TCAGA 8233130 1.4565163 8.082303 130-139
ATCGG 11139255 1.4543017 9.82608 190-199
AGCTC 9929975 1.4539387 8.668154 140-149
CAATA 6049290 1.45013 7.674639 100-109
GGCCG 15587060 1.4371614 11.716488 120-129
GAATT 7770990 1.436586 11.058804 170-179
CCATC 8678280 1.4250537 11.20813 240-247
CCACC 9050075 1.4221483 8.349369 230-239
TCCAC 8647755 1.4200413 9.675306 240-247
CTCGA 9688460 1.4185765 8.41145 190-199
CTGGT 12407500 1.40099 6.5138574 160-169
ACCAT 7021650 1.3931223 10.641295 45-49
CGTAG 10661010 1.3918637 8.230132 210-219
TGGAC 10599605 1.3838469 10.473278 170-179
CAGAC 8137785 1.3776906 12.284256 60-69
GAGGC 12356240 1.3765124 26.124989 30-34
GAGGG 13844365 1.3752043 8.434384 200-209
ACTAT 6575080 1.3631887 7.0439425 230-239
AAGTA 6294375 1.3454126 17.778938 70-79
ACACG 7926370 1.341899 9.0249815 120-129
GGCTC 12411430 1.3411202 8.579698 130-139
GGGCC 14530695 1.3397623 32.244267 15-19
TTCCA 7750445 1.3299311 8.060823 230-239
GGGAG 13346420 1.3257418 6.36131 150-159
CTTGA 8664415 1.3256903 8.744305 200-209
TAGTT 8287435 1.3250366 8.319548 220-229
GGTGG 15400910 1.323101 41.182423 25-29
CATGT 8614965 1.3181245 10.68869 70-79
TATAC 6266130 1.2991352 6.510902 120-129
GTCCA 8866520 1.2982285 12.270163 70-79
TGGGC 13446320 1.2955366 34.0965 15-19
TATGG 9494595 1.2953278 6.4761696 190-199
GGAGT 11106755 1.2929624 7.559864 210-219
TATCA 6183850 1.2820764 5.87453 70-79
AAAGT 5989210 1.280184 21.479935 70-79
CCATG 8737820 1.2793846 10.863242 70-79
CCTCA 7773330 1.2764528 6.124981 130-139
GTGTT 10706770 1.2633222 7.527294 160-169
AGAGA 6924710 1.2629899 13.1109085 35-39
ACTTC 7342765 1.2599757 5.766542 220-229
AGAGT 7985100 1.2595956 5.1859255 210-219
ACTCG 8602295 1.2595412 8.618196 190-199
ACGTG 9646110 1.2593619 8.872487 170-179
GAACC 7436675 1.2589958 8.989829 70-79
GGAAT 7813510 1.2325284 8.851262 170-179
ATCAT 5932890 1.2300457 5.41055 90-99
GTGCT 10881475 1.2286793 6.8234024 220-229
GCGTA 9311820 1.2157183 9.39164 210-219
CAGTG 9274145 1.2107995 5.1624365 160-169
ACAAT 5049945 1.210568 8.628641 100-109
TCACG 8233760 1.2055802 9.026569 200-209
TGATA 6498795 1.2014014 6.1734767 180-189
TTGAG 8763740 1.1956187 7.8676267 200-209
AGTTC 7764875 1.1880572 5.952285 160-169
CTGCG 10987480 1.1872549 6.5766296 210-219
GCTCA 8079600 1.1830086 8.3398905 140-149
AGTGA 7405145 1.1681116 5.031858 160-169
TGCCC 9605345 1.1640147 10.276376 60-69
TGTGC 10302450 1.163299 6.0656495 220-229
CTGAT 7475775 1.1438236 5.6436496 180-189
GCATA 6379065 1.1285154 7.3848186 210-219
ACTGG 8500795 1.1098337 6.5812273 160-169
TATGT 6934970 1.1087978 7.3285737 220-229
CGGCT 10205350 1.1027418 7.7855783 130-139
CTCGG 10191655 1.101262 7.938682 130-139
AAAAG 4455265 1.1010929 13.51791 70-79
ATCCA 5502215 1.0916606 14.306122 240-247
GTCTA 7046575 1.0781542 7.2221146 180-189
GCGGT 11141150 1.0734364 6.5955176 130-139
TTGGC 9501180 1.0728236 5.1962304 170-179
ATAAC 4474775 1.0726887 7.234458 80-89
TATAA 4254990 1.0658742 7.687048 240-247
TGGTC 9339745 1.0545952 8.725152 50-59
CTGAA 5946420 1.0519762 14.641384 35-39
CCCAA 5539265 1.0517131 8.763397 240-247
GTGGT 10351970 1.0422554 7.6072764 50-59
CGATG 7981320 1.0420129 24.416807 15-19
CATCA 5237290 1.0390985 6.5254416 240-247
TCTAC 6036105 1.0357603 6.7770505 180-189
AGGTT 7540395 1.0287204 16.705008 15-19
CGGAG 9207435 1.0257287 7.2040453 210-219
GAAGA 5619385 1.0249132 18.718983 35-39
TAGAT 5514290 1.0194007 7.430304 240-247
CCAAT 5127010 1.0172185 10.066873 240-247
AAAAT 3458550 1.0017262 5.949436 5
CCATT 5803630 0.9958691 5.0611286 50-59
CGGTC 9145205 0.98818755 6.6960998 130-139
GAGTT 7098335 0.968411 5.273032 160-169
ACCGT 6587855 0.9645882 13.597818 45-49
ATACG 5427595 0.96019137 5.105784 120-129
ATATC 4606570 0.9550643 5.9446473 70-79
TGTAA 5157450 0.9534333 6.1509347 230-239
AAGAG 5199030 0.9482452 12.958041 35-39
AGAGC 6279020 0.9478459 5.4883165 140-149
TAGAC 5325200 0.94207686 5.009248 110-119
ACTAA 3881355 0.93043464 11.588818 240-247
GCTTC 7330465 0.9282864 5.1102023 150-159
ATCAA 3812580 0.913948 13.42435 70-79
TGAAG 5751975 0.9073352 10.350887 35-39
CTACC 5462145 0.8969347 6.728265 230-239
AGCTT 5844515 0.89423436 5.3504963 150-159
CACAA 3874390 0.8887937 5.4245124 110-119
CTTCC 6250910 0.88775533 5.599751 230-239
TGCGT 7586145 0.8565878 6.8214755 210-219
CCTTT 5694650 0.84512705 6.34289 50-59
TGCAT 5506590 0.84253037 6.0284753 210-219
AGCTG 6379065 0.8328281 7.634113 30-34
ACCCA 4384645 0.83249116 11.219176 240-247
TACAA 3446640 0.8262252 5.165479 100-109
CCAGC 5848835 0.8195254 5.6074224 80-89
AGCCA 4833785 0.81833816 7.8153186 80-89
TAACC 4028660 0.7993015 6.624082 80-89
AAAGA 3192605 0.78903383 5.240222 70-79
TGAAC 4350755 0.7696885 5.413774 190-199
CGTTG 6812335 0.7692133 5.0087204 170-179
AAAGG 4181880 0.7627284 7.5295486 10-14
TCGAA 4247005 0.75133413 13.14471 70-79
TGAGA 4756675 0.7503333 5.676798 240-247
AGGAT 4747530 0.7488908 9.112795 10-14
GGAAG 5525530 0.7437384 10.360945 30-34
CTAAT 3546015 0.73518306 9.259443 240-247
AATCC 3684860 0.73109037 8.96909 240-247
GGTCC 6738000 0.72807634 9.918368 50-59
ACGAT 4112245 0.72749394 9.229885 10-14
GAAGT 4553315 0.7182547 5.4319634 70-79
ACGTC 4903240 0.71792835 7.8635235 70-79
ATACC 3590460 0.712361 5.6709023 240-247
TAGGT 5201540 0.70963526 5.5447865 10-14
GTCGA 5318635 0.6943821 8.848573 70-79
GCGTT 6148800 0.6942904 5.07519 170-179
GACCG 5497865 0.6868905 14.1183195 45-49
CGAAC 4004885 0.6780092 8.923627 70-79
CAATC 3398940 0.6743628 8.991724 240-247
TGGAT 4913690 0.6703645 6.927673 10-14
AATGG 4225380 0.66652524 5.462567 10-14
CTTTG 4998705 0.66147405 5.480216 50-59
AAAGC 3202870 0.6551448 9.008077 70-79
TTTCG 4907365 0.6493872 5.1032953 100-109
CGTGG 6704475 0.64596814 5.2457647 50-59
CGTCC 5156550 0.62489176 6.3693695 70-79
AAGTC 3479465 0.6155493 5.0070066 70-79
GAAGC 4049640 0.61131114 9.434855 30-34
AAGAA 2463880 0.6089337 5.170873 7
GATGA 3850580 0.607403 5.1845593 45-49
ATAGG 3803405 0.59996146 5.4373627 10-14
AAGGT 3803055 0.59990627 6.0057025 15-19
TTGCC 4708940 0.59631205 6.791285 60-69
TATGA 3223785 0.59596586 5.0185065 240-247
AAGCT 3199790 0.56607234 15.353949 30-34
CATCC 3432310 0.563617 6.56337 240-247
GACGA 3679635 0.55545723 7.4972153 40-44
TCAAG 3027825 0.53565 5.7154274 70-79
AAGCA 2419960 0.4950011 5.8102083 70-79
GGGAT 4226945 0.49206817 6.2568145 10-14
CGGTT 4155235 0.46918747 8.742088 10-14
TCGAT 2891690 0.44244018 6.8650002 10-14
GCGAT 2705150 0.3531748 5.98243 10-14