FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_16_SL254626.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_16_SL254626.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4144682
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 4667870 8.0632305 56.69598 110-119
CACAG 6154245 6.9499006 39.85622 110-119
TCCCT 6627280 6.338161 90.65739 30-34
CAGTA 5147930 6.1992455 39.555813 60-69
ATACA 3808785 6.1698465 30.514584 110-119
GGAGA 6590355 6.0999227 91.58065 35-39
GGTGA 7146040 5.7915654 92.62542 45-49
AGTAA 3868045 5.67264 41.957485 110-119
CCCTG 6637920 5.3896847 54.505646 30-34
GTAAT 4175945 5.3624525 36.529247 110-119
GTAGT 5152980 4.919099 24.697369 70-79
AGACG 4811010 4.9186497 97.4374 40-44
ACAGT 4046135 4.8724413 32.990715 110-119
GGCCC 6982515 4.8133407 55.3269 60-69
GTGAC 5323180 4.765361 81.99061 45-49
GAGAC 4652690 4.756788 127.216095 40-44
CCCCA 4564065 4.6747923 96.29597 60-69
GTGTC 5552405 4.3523192 24.919535 120-129
ACTCC 3948995 4.313199 91.98667 30-34
AATAC 2628805 4.2583976 30.73526 110-119
ACCAG 3757270 4.243031 91.5841 45-49
CGTGT 5378655 4.2161226 24.145496 120-129
GCACA 3717285 4.1978765 32.936073 110-119
TAGTA 3247845 4.1706524 29.022135 80-89
CCCTT 4344480 4.154949 62.199867 20-24
TGACC 4145740 4.0994053 147.64116 45-49
TGGTG 5635665 3.9993613 28.645882 25-29
GACCA 3445365 3.8908012 102.67626 45-49
CTCAG 3932900 3.888944 29.139154 140-149
AGTAG 3561580 3.8828874 26.585674 70-79
CCCAG 4187015 3.8825836 80.860466 60-69
CACTC 3550795 3.8782744 78.51483 25-29
TGAGG 4738880 3.840663 69.29187 35-39
CCACT 3465850 3.785495 26.279905 240-247
CTTGG 4665235 3.6569006 37.042255 20-24
CTCCC 4053130 3.635097 55.609924 30-34
AGGAG 3924725 3.63266 90.96808 35-39
GACGG 4762395 3.619541 110.10805 40-44
CAGGG 4716695 3.5848076 32.904816 50-59
CTGAG 3999910 3.5807571 96.29112 35-39
ATGGT 3722625 3.5536642 25.390047 190-199
TGTCT 3832235 3.5382485 20.944906 180-189
TCTTG 3810310 3.5180058 19.540287 170-179
ACGGT 3929595 3.5178106 92.38893 40-44
AAAAA 1552675 3.49817 19.167051 240-247
GCCCC 4564105 3.4752376 71.31627 60-69
AGATA 2356180 3.4554305 18.073614 150-159
AGGCT 3777395 3.3815596 43.342236 30-34
GAGGA 3610015 3.3413696 126.30668 35-39
CTTCA 2847915 3.3169768 16.804487 200-209
TGTCC 3781915 3.274504 18.941732 120-129
GCCCT 4016120 3.2609036 32.880142 20-24
ATGCA 2706810 3.2595978 78.38719 25-29
TCAGG 3571070 3.1968555 19.90291 140-149
GATGG 3940100 3.1932855 33.074932 15-19
CCAGG 3797975 3.1884108 37.715797 50-59
GGGTT 4467345 3.170261 42.19638 50-59
CTCTC 3299065 3.1551414 13.345601 100-109
CCTGG 4273485 3.141372 30.939325 50-59
CCGTG 4264560 3.1348116 23.961725 120-129
TTCCC 3263605 3.1212277 52.948807 50-59
GGATG 3780300 3.063774 57.844563 20-24
ACAGA 2214265 3.0452356 14.408232 140-149
TGCAC 3038975 3.00501 101.27583 25-29
TGGAG 3669770 2.9741943 31.332933 25-29
TCGCA 2981335 2.9480143 23.728046 100-109
CCAGT 2979280 2.945982 39.059544 60-69
TCTCA 2520075 2.9351404 20.447325 130-139
TACAC 2200510 2.9270072 21.906633 110-119
GGTTC 3730555 2.9242404 47.08582 50-59
GACTC 2930750 2.8979943 20.166782 190-199
TCTCT 2830740 2.8868906 15.616044 180-189
CGCAC 3111990 2.8857222 23.81492 100-109
CAGAT 2346055 2.8251688 15.533582 150-159
TTCTT 2591900 2.818714 21.703857 170-179
TGGCC 3810580 2.8010976 37.993565 60-69
GCCGT 3780250 2.7788026 22.635544 120-129
CGGAT 3079325 2.756641 64.83343 20-24
GGTTG 3871435 2.747372 60.08744 15-19
GCACT 2772550 2.7415624 71.98534 25-29
AGGGT 3379535 2.7389712 49.598476 50-59
CCTGA 2768695 2.7377505 64.30174 30-34
CGGTG 4076700 2.7130125 67.53597 40-44
GTTCC 3115055 2.6971152 53.234123 50-59
TCACA 2009260 2.6726155 11.950904 140-149
AGGGA 2866980 2.6536288 9.561385 150-159
ACCAC 2121725 2.6465921 15.068618 230-239
AATAA 1339110 2.6417491 12.517732 110-119
GGCTG 3879940 2.5820704 24.245266 30-34
AGTCA 2144180 2.5820668 28.116344 70-79
CGGCC 3720870 2.5649517 10.717879 120-129
TCAAA 1572900 2.5479386 52.294895 70-79
GTTGG 3572240 2.5350475 46.692425 15-19
AGATG 2316500 2.5254827 16.900944 180-189
TGTTC 2684250 2.4783301 13.091802 140-149
TCCAT 2127680 2.4781163 13.750287 70-79
GATGC 2755280 2.4665527 75.357056 25-29
GAGAT 2245145 2.4476905 16.562279 180-189
CTGGC 3257990 2.3948977 33.8094 60-69
GTCTC 2745305 2.3769739 12.630881 180-189
ACTCT 2032680 2.367469 12.684685 200-209
TTGTC 2517845 2.3246908 14.974774 170-179
GTTCT 2508470 2.316035 18.24744 160-169
CAGAG 2264940 2.315615 9.222014 140-149
TCATT 1860530 2.3107579 16.40777 150-159
TCCTC 2410990 2.305809 14.837919 130-139
CATTT 1852550 2.3008468 17.570486 150-159
TTCAT 1852060 2.3002381 17.066536 140-149
ACAGC 2028440 2.2906883 12.212881 120-129
CTGGA 2558545 2.290434 15.155332 180-189
TGTAG 2378855 2.2708845 8.608869 210-219
GGCGG 3931555 2.2213185 36.8919 20-24
TTGGT 2637265 2.2044277 41.28786 25-29
GTGAA 2018195 2.2002661 16.964024 190-199
TTCAC 1888755 2.1998394 17.40916 200-209
GCGGA 2860390 2.1739688 77.60526 20-24
TGAAT 1671400 2.1462934 14.88407 190-199
ATTTG 1905780 2.142873 15.534599 150-159
CACTA 1602705 2.131837 14.439371 240-247
ATATA 1224325 2.1148863 10.314955 110-119
CTCTG 2440895 2.1134057 14.905569 180-189
GAATC 1752725 2.1106684 15.330082 190-199
TTGGA 2201925 2.101985 16.661783 170-179
TCTGC 2416765 2.0925133 10.703448 210-219
TAGTC 1980625 2.088447 23.963783 70-79
GAGTC 2328515 2.0845087 12.675403 210-219
AATAT 1205760 2.0828173 12.229753 110-119
GTTCA 1973330 2.0807548 15.541115 140-149
GATAC 1726640 2.0792563 13.077788 150-159
GATAT 1604190 2.059987 9.491828 180-189
TCTCG 2372730 2.0543864 16.157427 100-109
ACTAC 1544175 2.0539832 12.314894 230-239
CAGCC 2201730 2.0416458 11.01723 120-129
TTGGG 2852115 2.0240092 73.54151 15-19
TCGGC 2739295 2.013613 8.71 190-199
CCTTC 2102770 2.011035 13.458104 200-209
ATATG 1564100 2.0085065 9.397297 180-189
CTACT 1723000 2.006784 8.320357 230-239
CCTTG 2297805 1.9895136 41.376156 20-24
CTCGC 2446810 1.9866967 16.37455 100-109
AATCG 1640110 1.9750551 13.926485 190-199
ACACA 1299035 1.9733598 13.37689 110-119
AATAG 1343495 1.9702882 8.22326 240-247
TTTGC 2114275 1.9520802 11.952939 150-159
ATAGA 1329740 1.9501162 9.833321 240-247
GTCAA 1611695 1.9408371 37.91986 70-79
TACAG 1603910 1.9314625 11.664542 160-169
TTGCA 1830940 1.9306134 13.646031 150-159
GTTGT 2304235 1.9260558 7.546759 210-219
CACGG 2278990 1.9132186 7.3451405 200-209
GAACT 1583785 1.9072272 11.7026205 160-169
TCCCA 1740135 1.9006225 11.18085 240-247
GTCAC 1921980 1.900499 9.232733 140-149
GCTGA 2087695 1.868924 37.620712 30-34
GCTGT 2374920 1.8616093 10.013405 140-149
GTCCT 2145205 1.8573877 12.82436 130-139
GGTCA 2061565 1.8455324 9.519662 130-139
TGGAA 1692500 1.8451886 9.2487 25-29
GTCTG 2341975 1.8357853 8.827429 210-219
AGTAT 1429025 1.8350526 11.781104 220-229
AGTCT 1737045 1.831607 12.908403 210-219
ATTGT 1628585 1.8311929 8.246154 170-179
TACTA 1285580 1.823484 10.540795 230-239
TACCA 1369860 1.8221183 11.681432 230-239
TCAGC 1839440 1.8188815 11.046504 140-149
CCAGA 1603160 1.8104256 24.147436 60-69
ATGTA 1408680 1.808927 11.853638 70-79
CAGGC 2140835 1.7972372 13.776465 140-149
CTGTT 1945465 1.7962205 12.754072 140-149
TGGGG 2979620 1.7951874 44.385723 15-19
CATAG 1469995 1.7701991 8.0060215 210-219
TTCAG 1654420 1.7444841 10.131155 150-159
CACCA 1389250 1.7329193 10.720206 230-239
ATCTC 1469515 1.7115492 6.7941446 100-109
TTGTA 1518245 1.7071257 9.787749 210-219
TCTGG 2175985 1.705672 12.23123 180-189
CTCCA 1560925 1.7048845 8.158611 240-247
GTGGA 2095665 1.6984484 38.708477 25-29
ATAGT 1312440 1.6853426 7.015976 220-229
GCAGA 1645530 1.6823466 13.729701 150-159
GACGT 1869500 1.673594 10.028698 170-179
CAGCT 1689580 1.6706963 9.216046 150-159
AGAAC 1206920 1.6598535 10.73778 160-169
CCCAC 1609320 1.648363 10.272117 240-247
TGCAG 1839685 1.6469034 12.73611 150-159
GGGGC 2909570 1.6438996 40.6319 15-19
GGAGG 2365695 1.6277688 26.960875 30-34
TGACT 1532090 1.6154946 11.793735 190-199
ACGGC 1924190 1.615363 11.999426 120-129
ATAAT 930490 1.6073189 5.5506296 100-109
GTCCC 1959580 1.5910885 17.869314 50-59
CCATC 1452590 1.5865581 10.639798 240-247
GAGAA 1270710 1.5821351 10.757188 160-169
ATAAA 800735 1.5796621 6.290907 110-119
GGACG 2077015 1.578584 7.893638 170-179
GGGCG 2777540 1.569303 47.83594 20-24
TGGTT 1873940 1.5663823 17.038898 10-14
CTCTT 1519595 1.5497378 9.087846 200-209
CACTG 1563815 1.546337 6.5920258 230-239
CAAAA 833190 1.5414054 19.435474 70-79
ATGGG 1901185 1.5408305 53.45696 15-19
CGACT 1556015 1.538624 9.173149 190-199
AACTG 1276210 1.536839 10.281463 160-169
AACCA 1008330 1.5317508 11.434869 70-79
ATCGG 1710330 1.5311036 9.682891 190-199
CAAAG 1104240 1.5186396 26.218605 70-79
TCGAC 1535800 1.5186352 9.666414 190-199
ACGGA 1473220 1.5061814 9.727118 200-209
ACACG 1331090 1.5031809 9.175596 120-129
ACTGA 1247835 1.5026693 7.489488 180-189
AGCTC 1519155 1.502176 9.224794 140-149
CCTCG 1827675 1.4839876 9.155613 130-139
GCTCT 1709210 1.4798889 9.353026 130-139
CTGGT 1874220 1.4691299 6.9686456 160-169
GTCCA 1479530 1.4629941 12.765532 70-79
AAATA 739450 1.458761 5.058991 110-119
TCAGA 1207235 1.4537779 8.780861 130-139
CCACC 1417200 1.4515821 7.979427 230-239
TGGAC 1614080 1.4449396 10.890879 170-179
GAGGG 2092375 1.4397048 9.306631 200-209
AGCCG 1713755 1.4387022 10.684877 120-129
CAGAC 1272200 1.4366771 13.006187 60-69
CTTGA 1356830 1.4306939 9.455714 200-209
TCCAC 1295015 1.4144504 9.772159 240-247
CTCGA 1427770 1.4118125 8.961576 190-199
GGCCG 2238030 1.3967109 11.576789 120-129
CGTAG 1555375 1.3923864 8.0653105 210-219
AATTG 1081205 1.3884069 9.11408 170-179
TAGTT 1229545 1.3825094 8.715799 220-229
GGGAG 1973275 1.3577554 6.728512 150-159
CATGT 1283645 1.3535246 11.290025 70-79
TATGG 1403010 1.3393308 6.694176 180-189
CAATA 823570 1.3340999 7.4422703 100-109
GAGGC 1753920 1.3330237 23.571798 30-34
GGCTC 1805435 1.3271471 8.322335 130-139
GAATT 1027820 1.3198535 10.087207 170-179
ACTAT 926440 1.314075 7.1981735 230-239
GTGCT 1641260 1.2865213 7.3236065 220-229
ACCAT 966590 1.285709 10.313473 45-49
GGAAT 1173660 1.2795416 8.24722 170-179
ACGTG 1429195 1.2794288 9.457228 170-179
ATCCA 961815 1.2793576 15.185959 240-247
CCATG 1293675 1.2792163 11.198686 70-79
GGTGG 2121920 1.2784328 39.11794 25-29
GTGTT 1529045 1.2780927 7.428376 160-169
ACTCG 1287380 1.2729915 9.078845 190-199
ACTGT 1197395 1.2625793 5.2669435 230-239
TATAC 887715 1.2591469 6.243832 110-119
CTGAA 1043905 1.2570925 13.620572 35-39
TTCCA 1076165 1.2534131 7.4217496 230-239
ACTTC 1071480 1.2479563 5.5507293 220-229
TGATA 967215 1.242029 6.860813 180-189
GGGCC 1987955 1.240644 29.69641 15-19
AAGTA 845825 1.2404357 15.984937 70-79
CCTCA 1134195 1.2387984 5.765284 130-139
AGAGA 992780 1.2360902 12.613314 35-39
GGAGT 1516495 1.2290555 6.9597783 210-219
GCTCA 1226490 1.212782 8.952112 140-149
TTGAG 1266935 1.2094319 8.639406 200-209
TGGGC 1813700 1.2070034 32.020927 15-19
GCGTA 1347040 1.2058829 9.001019 210-219
TGTGC 1532505 1.2012725 6.151202 220-229
AAAGT 817865 1.1994313 19.63517 70-79
GAAGA 962555 1.1984576 18.086882 35-39
AGTTC 1132645 1.1943045 5.967461 160-169
TATCA 833420 1.1821342 5.593582 70-79
GAACC 1044675 1.1797364 8.5760355 70-79
AGATC 979155 1.1791191 5.611267 130-139
TGCCC 1432185 1.1628681 10.424411 60-69
CTGAT 1101170 1.161116 6.010019 180-189
CTGCG 1563545 1.1493375 6.268654 210-219
AGTGA 1050205 1.1449491 5.0527306 160-169
AGAGC 1113020 1.1379224 6.5615053 140-149
ACTGG 1258395 1.1265271 6.942532 160-169
TCACG 1134920 1.1222355 8.5361805 200-209
GTCTA 1053625 1.1109828 7.6110497 180-189
GCATA 920465 1.1084433 7.3342786 210-219
AAGAG 888735 1.1065459 12.483562 35-39
TAGAT 857680 1.1013719 7.769751 240-247
TATGT 979470 1.1013232 7.27916 220-229
ACAAT 679420 1.1005915 8.34905 100-109
CCCAA 879125 1.0966008 9.224536 240-247
TTGGC 1386750 1.0870206 5.1222153 170-179
AGGTT 1132525 1.0811224 18.423494 10-14
TGGTC 1379150 1.0810633 9.487722 50-59
TCCAG 1089685 1.0775062 5.3711843 240-247
GTGGT 1512450 1.0733132 8.415827 50-59
AAAAG 637760 1.0681592 13.053033 70-79
GCGGT 1598790 1.0639824 6.8992205 130-139
CTCGG 1446840 1.0635495 7.5697546 130-139
CGGCT 1433700 1.0538905 7.5234494 130-139
ATATC 739330 1.0486758 5.548397 70-79
TCTAC 897815 1.0456883 7.158693 180-189
TATAA 597585 1.0322621 7.4441595 240-247
CCAAT 770680 1.0251194 10.35634 240-247
ATAAC 631225 1.0225207 6.680211 80-89
TCTGT 1083115 1.0000249 5.2484965 210-219
CATCA 741225 0.9859398 5.7069097 230-239
CGGTC 1327695 0.9759678 6.9358387 130-139
GAGTT 1021435 0.9750745 5.5721917 160-169
TACTG 923155 0.9734102 5.071385 180-189
ACCCA 777955 0.9704036 10.958685 240-247
ACCGT 975305 0.96440446 14.714666 45-49
CGGAG 1260880 0.9583007 6.447643 210-219
CGATG 1069410 0.9573459 22.301647 15-19
TGTAA 742065 0.9529072 6.379679 230-239
AGCTT 902655 0.951794 5.763408 150-159
GCTTC 1090870 0.94451046 5.394442 150-159
AAAAT 477360 0.94171906 5.1807175 5
ACTAA 575590 0.9323974 11.319974 240-247
TGAAC 762085 0.9177189 5.229535 190-199
TGAAG 834545 0.9098334 9.892434 35-39
CTTCC 949745 0.9083118 5.2087097 230-239
CCAGC 967045 0.8967327 5.8272705 80-89
ACGTC 901340 0.8912661 8.401852 70-79
ATCAA 537395 0.87052554 12.633887 70-79
AGACC 770380 0.869979 5.1077313 240-247
CTACC 796190 0.8696202 6.510443 230-239
GGAAG 938180 0.86836374 9.925541 30-34
CCTTT 850460 0.8673297 7.0210657 50-59
GTATG 899460 0.8586357 5.2718654 220-229
AGCTG 958390 0.85795975 7.1811886 30-34
GAGCT 947890 0.8485601 5.3520255 140-149
TGCAT 801250 0.8448688 6.0456204 210-219
AATCC 631985 0.8406344 9.728229 240-247
TGCGT 1068875 0.8378506 6.662932 210-219
AGCCA 709170 0.8008555 7.1864424 80-89
GACCG 943960 0.7924571 14.680602 45-49
AAAGG 629530 0.783815 7.2333717 10-14
CGTCC 944195 0.76664263 6.67896 70-79
CGTTG 976735 0.7656254 5.187475 170-179
TGAGA 689640 0.7518558 5.8290324 240-247
GGTCC 1014320 0.7456107 10.310719 50-59
CTAAT 523110 0.7419863 8.974404 240-247
TAGGT 772670 0.7376004 6.3442807 10-14
TAACC 548145 0.7291148 5.993687 80-89
TCGAA 595050 0.7165718 12.407401 70-79
AGGAT 654315 0.7133439 8.305629 10-14
CAATC 535755 0.71263415 9.753896 240-247
ATACC 532280 0.7080119 5.7281528 240-247
GCGGC 1125635 0.70248675 5.004335 130-139
GCGTT 889840 0.69751173 5.201321 170-179
CTTTG 752960 0.6951974 6.1779513 50-59
TGGAT 725525 0.6925952 6.931941 10-14
ACGAT 556855 0.6705765 8.879482 10-14
CGTGG 1003400 0.66775495 5.550064 50-59
GTCGA 744830 0.6667789 8.518244 70-79
AAGGT 601855 0.6561513 6.6001916 10-14
AATGG 598755 0.6527716 5.4864745 10-14
CGAAC 577580 0.65225273 8.623585 70-79
AAAGC 470715 0.64736515 8.927236 70-79
TTGCC 709125 0.6139833 7.0355625 60-69
CATCC 554280 0.60539955 6.830849 240-247
GAAGC 574990 0.5878546 8.856922 30-34
ATAGG 532745 0.58080655 5.576929 10-14
AAGCT 466730 0.5620461 14.607934 30-34
TCAAG 446620 0.5378292 5.670493 70-79
GACGA 500630 0.5118309 6.26537 40-44
CGGTT 624395 0.48943952 10.465235 10-14
AAGCA 347615 0.47806814 5.6719217 70-79
GGGAT 585255 0.47432458 6.0956955 10-14
TCGAT 405695 0.4277804 6.601012 10-14
GCGAT 358095 0.32057002 5.130703 10-14