FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_6_SL254633.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_6_SL254633.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 19248008
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 22761675 8.397795 58.33292 110-119
CACAG 29115070 7.2630787 41.85896 110-119
TCCCT 31364100 6.5593486 96.209305 30-34
CAGTA 25170805 6.555509 43.325417 60-69
ATACA 18241140 6.4462423 31.540812 110-119
GGAGA 31381340 6.207821 93.95881 35-39
AGTAA 18815210 5.921015 43.60074 110-119
GGTGA 33922900 5.7981496 93.8219 45-49
CCCTG 31764970 5.6663356 58.337227 30-34
GTAAT 20312780 5.5231285 37.247692 110-119
GGCCC 33517325 5.0997534 60.41304 60-69
AGACG 22705180 5.0438275 102.572914 40-44
ACAGT 19351710 5.039978 34.589504 110-119
CCCCA 21032640 4.8762565 104.82525 60-69
GTGAC 25215820 4.8399086 84.67431 45-49
GTAGT 24146990 4.8387527 24.699652 70-79
GAGAC 21442625 4.7633586 133.07707 40-44
ACCAG 18314800 4.568831 101.449036 45-49
AATAC 12493160 4.414962 31.942629 110-119
GTGTC 26521325 4.3983307 25.26145 120-129
CGTGT 25913765 4.2975726 24.120144 120-129
TGACC 19863415 4.2813973 155.1055 45-49
GACCA 16977135 4.235136 112.79616 45-49
GCACA 16934440 4.224485 35.480564 110-119
ACTCC 17112395 4.141999 95.53372 30-34
CCCTT 19643425 4.108138 61.737026 20-24
TAGTA 15021510 4.08441 25.146378 80-89
CCCAG 19243675 3.9729507 86.2532 60-69
CTCAG 18257385 3.9352307 30.77732 140-149
TGGTG 26543315 3.9199476 27.098732 25-29
AGTAG 16691340 3.8710868 28.543848 70-79
CCACT 15993090 3.871075 27.370932 240-247
CACTC 15856630 3.8380451 81.38906 25-29
TGAGG 22412350 3.8307502 71.14742 35-39
CTCCC 18790675 3.7641234 60.33164 30-34
CAGGG 22937395 3.7552087 35.926876 50-59
GACGG 22881375 3.7460375 113.86767 40-44
CTGAG 19075970 3.6614294 99.94634 35-39
AGGAG 18505970 3.6608303 93.66195 35-39
CTTGG 22042955 3.6556323 35.557785 20-24
GCCCC 21290595 3.6377697 76.99135 60-69
ACGGT 18807925 3.6099803 94.73014 40-44
TCTTG 18409115 3.579312 20.512484 170-179
TGTCT 18346380 3.5671146 22.144644 180-189
ATGGT 17720065 3.5508778 25.999256 190-199
AGATA 11259010 3.5431316 19.888567 150-159
GAGGA 17409275 3.443883 131.01395 35-39
CCAGG 18712330 3.440214 41.685997 50-59
AGGCT 17687255 3.39488 43.272125 30-34
GCCCT 18799760 3.3535604 32.883877 20-24
TGTCC 17995650 3.3514116 19.80619 120-129
CTTCA 13083010 3.3060791 18.03407 200-209
TTCCC 15745900 3.2930272 58.270355 50-59
CTCTC 15717005 3.2869847 13.992736 100-109
ATGCA 12568590 3.2733757 79.989426 25-29
GGGTT 21995870 3.2483757 44.665916 50-59
TCAGG 16774050 3.2196004 20.322456 140-149
CCTGG 20058025 3.1862102 33.007706 50-59
CCGTG 19941440 3.167691 24.496317 120-129
GATGG 18490145 3.1603615 31.62577 15-19
ACAGA 10423020 3.141769 14.517014 140-149
TCGCA 14553295 3.1368442 25.159643 100-109
CGCAC 14951475 3.086805 25.632143 100-109
GGTTC 18464400 3.06216 50.674084 50-59
TGCAC 14030010 3.0240543 103.62966 25-29
TCTCT 13821105 3.0177069 16.994545 180-189
TACAC 10227140 2.9911013 22.599466 110-119
GGATG 17462665 2.984743 57.135597 20-24
TCTCA 11784545 2.9779568 20.905298 130-139
TGGCC 18704290 2.9711702 40.865215 60-69
CCAGT 13701680 2.9532855 42.90008 60-69
TGGAG 17269415 2.9517126 30.423721 25-29
GACTC 13604565 2.9323528 20.061407 190-199
TTCTT 12677795 2.8899107 22.935608 170-179
GTTCC 15488090 2.8844173 58.0627 50-59
CCTGA 13325820 2.8722718 68.62225 30-34
AGGGT 16655395 2.8467631 52.889587 50-59
AATAA 6654865 2.8416572 13.027196 110-119
ACCAC 10136195 2.8395233 16.377619 230-239
TCAAA 7954070 2.8108914 58.08693 70-79
GCCGT 17620840 2.7990642 23.24622 120-129
TCACA 9553590 2.7941103 12.44253 140-149
GCACT 12896480 2.779731 73.42841 25-29
CGGTG 19603340 2.7729928 68.66495 40-44
CAGAT 10640265 2.771161 16.374924 150-159
CGGAT 14295940 2.7439532 64.97816 20-24
CGGCC 18006530 2.739743 11.665246 200-209
GGTTG 18243635 2.6942413 58.550507 15-19
AGTCA 10113635 2.634005 31.182686 70-79
AGGGA 13222400 2.6156402 9.464523 150-159
GGCTG 18331910 2.5931425 24.251907 30-34
AGATG 11172465 2.591139 17.645397 180-189
TGTTC 13268095 2.579736 13.643815 140-149
CTGGC 15970215 2.536863 36.73927 60-69
GAGAT 10750480 2.4932716 17.504257 180-189
GTTGG 16858935 2.489747 45.847206 15-19
GATGC 12917025 2.4792852 75.66275 25-29
TCATT 9259155 2.4427748 18.181353 150-159
ACAGC 9703990 2.4207685 13.024414 120-129
CATTT 9149495 2.4138436 19.188139 150-159
GTCTC 12866630 2.396211 13.860928 180-189
GTTCT 12303315 2.3921523 18.756676 160-169
TTCAT 9053180 2.388434 18.158026 140-149
TTGTC 12228930 2.3776896 15.381956 170-179
ACTCT 9244185 2.3360074 13.13637 200-209
AATAT 6305815 2.3264961 13.064712 110-119
TCCTC 11108000 2.323078 16.14306 130-139
CAGAG 10408885 2.3122752 9.26874 140-149
CTGGA 12020890 2.307282 16.069399 180-189
CACTA 7876715 2.3036795 16.01584 240-247
ATATA 6221340 2.2953293 11.049799 110-119
TTCAC 9057325 2.288788 18.794127 200-209
GTGAA 9681270 2.2452986 18.428993 190-199
ACTAC 7606715 2.2247133 12.863906 230-239
GGCGG 18403370 2.2204552 37.158073 20-24
TAGTC 9860785 2.21896 26.144878 70-79
ATTTG 9436965 2.2170565 16.503822 150-159
GAATC 8481645 2.208968 16.58893 190-199
TCCAT 8667080 2.1901731 11.370088 70-79
TGAAT 8005895 2.1768358 15.955423 190-199
TTGGA 10860195 2.1762464 17.413414 170-179
GCGGA 13193305 2.1599495 78.48074 20-24
TGTAG 10751790 2.1545236 8.7071 210-219
GTCAA 8262590 2.1519172 42.50684 70-79
GAGTC 11183855 2.1466217 13.757349 210-219
TTGGT 12377955 2.1431274 38.984398 25-29
GATAC 8211610 2.1386397 14.212521 150-159
CTCTG 11447055 2.1318374 15.93913 180-189
TCGGC 13405415 2.1294456 9.055182 190-199
TCTGC 11365075 2.1165695 11.37646 210-219
GTTCA 9364350 2.107248 16.569244 140-149
CTACT 8273295 2.0906634 8.844078 230-239
CTCGC 11709305 2.0887432 17.21571 100-109
AATCG 8013455 2.087032 14.793832 190-199
CAGCC 9969825 2.058319 11.831255 120-129
CCTTC 9834150 2.0566702 14.842546 200-209
CACGG 11166245 2.0528855 8.175032 200-209
TACAG 7877300 2.0515716 13.000713 160-169
ATATG 7451910 2.0262048 9.444967 180-189
AATAG 6437805 2.025932 8.724398 240-247
TCTCG 10869565 2.0242884 16.741026 100-109
ACACA 5925485 2.0057282 13.774672 110-119
TTGCA 8862945 1.9944174 14.642679 150-159
TTGGG 13403600 1.9794592 71.794495 15-19
GATAT 7205845 1.9592987 9.277549 180-189
CCTTG 10464285 1.9488113 40.51158 20-24
ATAGA 6183905 1.9460316 9.568409 240-247
GTCCT 10375010 1.9321853 13.988314 130-139
TGGAA 8327540 1.9313389 9.778896 170-179
TACTA 6309745 1.9266158 10.981449 240-247
TACCA 6577305 1.9236449 12.784842 230-239
GCTGT 11596420 1.9231656 10.515868 140-149
TTTGC 9863685 1.9178113 12.718661 150-159
AGTAT 7047955 1.9163678 12.4917555 220-229
ATTGT 8104650 1.9040514 8.994169 170-179
GTTGT 10959070 1.8974607 7.544835 210-219
AGTCT 8406655 1.891739 14.17126 210-219
TCCCA 7808315 1.8899771 11.81092 240-247
CAGGC 10211795 1.8774122 14.293358 140-149
GCTGA 9729760 1.8675239 37.593914 30-34
GGTCA 9672860 1.8566025 9.637291 130-139
CTGTT 9526140 1.8521819 13.332741 140-149
CATAG 7018305 1.8278542 7.982474 210-219
GTCAC 8408900 1.8124698 9.593923 140-149
GTCTG 10642105 1.7649004 9.54464 210-219
AAAAA 3570905 1.7647456 6.833071 4
TCTGG 10638555 1.7643118 13.108008 180-189
GAACT 6746755 1.7571315 10.761945 160-169
GCAGA 7895465 1.7539331 14.76767 150-159
TGGGG 13891295 1.7498218 43.31892 15-19
ATAGT 6418525 1.7452233 7.431436 220-229
TTGTA 7335420 1.723334 9.7444725 210-219
AGAAC 5698445 1.7176592 11.054708 160-169
AACCA 5073140 1.7172167 13.083326 70-79
TCAGC 7966600 1.7171357 11.015482 140-149
ACGGC 9281960 1.7064644 12.474384 120-129
CAGCT 7866640 1.6955901 9.354102 150-159
ATAAT 4574680 1.6878033 5.8255553 100-109
TGCAG 8766245 1.6825875 13.492437 150-159
CACCA 5998590 1.6804271 11.324447 230-239
CAAAG 5513720 1.6619785 31.071234 70-79
ATAAA 3873405 1.6539613 7.1693397 8
GGGGC 13568465 1.6371009 40.59448 15-19
GTGGA 9556050 1.6333334 37.148224 25-29
ATGTA 5992380 1.6293527 9.646549 70-79
TTCAG 7216480 1.6239156 10.106652 150-159
ACGGA 7293540 1.6202188 10.635782 200-209
ATCTC 6410760 1.6200004 7.005089 100-109
GGAGG 11111910 1.6199853 26.197042 30-34
CCCAC 6959855 1.613589 10.754745 240-247
GAGAA 5999010 1.6102477 11.038226 160-169
GGACG 9806205 1.6054287 7.9749584 170-179
AACTG 6136855 1.5982885 10.414527 160-169
GACGT 8321150 1.5971558 9.916335 170-179
CAAAA 3903675 1.5966128 20.214 70-79
TGACT 7079245 1.5930337 11.5206 190-199
GGGCG 13089510 1.5793126 47.863617 20-24
CGACT 7286365 1.5705166 9.584127 200-209
CTCTT 7155980 1.5624402 9.344156 200-209
TCGAC 7223745 1.5570194 10.038479 190-199
CCTCG 8676320 1.5477093 9.865923 130-139
TGGTT 8917200 1.5439299 15.620012 10-14
AAATA 3615035 1.5436362 6.569346 6
AGCTC 7142880 1.5395894 9.580026 140-149
CACTG 7138185 1.5385774 6.4397097 230-239
GCTCT 8176695 1.522783 9.85602 130-139
AATTG 5579945 1.51721 10.034212 170-179
GTCCC 8495240 1.5154078 16.047897 50-59
ACTGA 5771935 1.5032483 7.4849296 180-189
CCAGA 6025150 1.5030409 22.411247 60-69
AGCCG 8157080 1.4996582 11.373268 120-129
CAATA 4234155 1.4963093 8.138138 100-109
ATGGG 8713005 1.489239 51.16029 15-19
CTCCA 6058045 1.4663299 7.9232807 240-247
ATCGG 7573355 1.4536247 10.118552 190-199
GGCCG 10722060 1.4527487 12.001208 120-129
CCACC 6236230 1.4458221 8.511472 230-239
GAATT 5310545 1.4439589 11.283537 170-179
CTCGA 6671965 1.4380878 9.018276 190-199
CTGGT 8669320 1.4377314 6.8611927 160-169
TCAGA 5505045 1.4337394 7.9828935 140-149
TGGAC 7453845 1.4306862 10.848992 170-179
CGTAG 7411145 1.4224905 8.617026 210-219
TCCAC 5868125 1.4203606 10.14756 240-247
GAGGG 9729060 1.4183822 9.292326 200-209
ACTAT 4638565 1.4163382 7.2321324 230-239
CCATC 5741005 1.3895915 10.987665 240-247
ACACG 5517730 1.3764592 9.15232 120-129
GGGAG 9422265 1.3736552 6.750048 150-159
CTTGA 6077465 1.3676044 9.552227 200-209
TATAC 4430165 1.3527055 6.931197 120-129
TAGTT 5736425 1.3476766 8.6736765 220-229
GGCTC 8464920 1.3446496 8.839449 130-139
AAGTA 4234450 1.3325517 18.507486 70-79
AAAGT 4230530 1.3313181 23.101524 70-79
TATGG 6613385 1.3252391 6.7006574 190-199
ACCAT 4474205 1.3085575 9.888352 45-49
ACTCG 6061235 1.3064497 9.4582205 190-199
GAACC 5213860 1.3006555 9.93869 70-79
GGAGT 7605705 1.2999775 7.673539 210-219
CAGAC 5165480 1.2885865 10.969663 60-69
TTCCA 5096965 1.288004 7.954058 230-239
ACGTG 6682765 1.2826855 9.490415 170-179
GTGCT 7694005 1.2759839 7.404326 220-229
TATCA 4174280 1.2745734 5.734268 90-99
GAGGC 7750750 1.2689185 23.29574 30-34
TCACG 5866420 1.264459 9.328565 200-209
ACTTC 4987560 1.2603574 5.5810494 220-229
GTGTT 7237470 1.2531004 7.767869 160-169
GGAAT 5389655 1.249979 8.934728 170-179
ATCAT 4089130 1.2485738 5.9273095 90-99
GCGTA 6485155 1.2447563 9.760539 210-219
CATGT 5522440 1.2427077 9.115302 70-79
ACTGT 5522430 1.2427055 5.1357517 230-239
GCTCA 5763970 1.2423767 9.348486 140-149
AGAGT 5351430 1.2411137 5.1338644 210-219
TGATA 4554470 1.2383791 6.8847547 180-189
GGGCC 9133475 1.2375089 29.214394 15-19
GGTGG 9817225 1.2366302 37.143013 25-29
CCTCA 5096155 1.2335075 6.0909276 130-139
TTGAG 6104555 1.223276 8.673231 200-209
CTGCG 7633545 1.2125859 7.0370636 210-219
GTCCA 5580925 1.2029229 10.7793665 70-79
CCATG 5555615 1.1974676 9.2741 70-79
TGGGC 8406665 1.189166 30.93626 15-19
AGAGA 4412500 1.1843983 12.195412 35-39
AGTTC 5249755 1.1813457 6.0703835 160-169
ACAAT 3331075 1.1771697 9.059702 100-109
TGTGC 7056890 1.1703237 6.1962295 220-229
CTGAT 5188345 1.1675268 6.1167116 180-189
ACTGG 5971710 1.146206 6.9608397 160-169
GTCTA 5068000 1.1404457 7.7592635 180-189
CAGTG 5937645 1.1396676 5.0320916 160-169
TATGT 4772995 1.121335 7.6706605 220-229
AGTGA 4816695 1.117097 5.130622 160-169
GCATA 4287170 1.1165547 7.5070105 210-219
CGGCT 6998920 1.111776 7.950878 130-139
CTCGG 6990385 1.1104202 8.10868 130-139
ATCCA 3760915 1.0999436 15.153694 240-247
TATAA 2973570 1.0970824 7.709149 240-247
ATAAC 3096975 1.094441 7.868331 80-89
GCGGT 7729625 1.093395 7.066063 130-139
TCTAC 4307050 1.0883925 7.3690863 180-189
CCAAT 3721020 1.0882758 10.8808 240-247
TGCCC 6092170 1.0867406 9.112015 60-69
AAAAG 2937905 1.0700307 13.153426 70-79
AGGTT 5325935 1.0672503 18.251589 10-14
CCCAA 3800365 1.064623 9.757367 240-247
TTGGC 6282105 1.0418324 5.416867 170-179
CTGAA 3979730 1.0364848 13.254434 35-39
CGGAG 6330550 1.0364097 7.364877 210-219
TAGAT 3798720 1.0328875 7.8060145 240-247
TCATA 3360115 1.0259767 5.114048 90-99
ATATC 3346215 1.0217324 5.3981566 70-79
CATCA 3451490 1.0094471 6.4860945 240-247
GAAGA 3744275 1.0050341 17.589964 35-39
CGGTC 6308755 1.0021435 7.120479 130-139
AGAGC 4487305 0.9968295 6.322974 140-149
TGGTC 5959415 0.98831713 7.553263 50-59
GTGGT 6683165 0.9869776 6.5677896 50-59
ATACG 3779480 0.9843315 5.3590937 120-129
AAAAT 2296600 0.9806586 5.2871118 5
GAGTT 4860330 0.9739489 5.632001 160-169
TGTAA 3576860 0.9725629 6.568869 230-239
CAGCG 5250430 0.9652781 5.2387404 160-169
GCTTC 5182680 0.96519405 5.567458 150-159
TACTG 4275705 0.9621565 5.1009283 180-189
GCCAC 4638375 0.9576152 5.0956125 80-89
AGCTT 4254085 0.95729136 6.015601 150-159
CTACC 3945885 0.95508856 7.2899714 230-239
CGATG 4960195 0.9520565 21.799574 15-19
ACTAA 2638505 0.93242216 12.413354 240-247
TAGAC 3571640 0.9302014 5.1734324 110-119
AAGAG 3414160 0.9164251 12.190905 35-39
ACCGT 4234660 0.9127464 11.984273 45-49
TGAAG 3823540 0.8867626 9.3423815 35-39
TGCGT 5308180 0.88031554 7.2246456 210-219
GAGCT 4555025 0.8742885 5.442351 140-149
ATCAA 2468295 0.8722716 12.569527 70-79
CTTCC 4127990 0.8633094 5.7567816 230-239
AGCTG 4384970 0.8416482 6.6190796 30-34
CACAA 2480775 0.8397221 5.0090523 100-109
ACCCA 2959000 0.82892543 11.273868 240-247
TGCAT 3674855 0.82694805 5.9777384 210-219
TAACC 2818770 0.8243973 7.312125 80-89
TACAA 2315650 0.8183283 5.5270653 100-109
CCAGC 3920345 0.8093744 5.6994815 80-89
AAAGA 2212865 0.80595976 5.4540644 70-79
AGCCA 3227500 0.8051359 7.8218765 80-89
CCTTT 3620835 0.7905749 5.638282 50-59
TCGAA 3005065 0.7826421 13.756778 70-79
AAATG 2463035 0.7750998 5.251008 9
TGAAC 2951760 0.7687593 5.438182 190-199
CGGCG 5634720 0.76345706 5.226792 130-139
AATCC 2590925 0.75776017 9.641035 240-247
GTATG 3776550 0.7567731 5.017315 220-229
AAAGG 2797770 0.75097436 7.8421035 10-14
CTAAT 2455920 0.74989 10.038841 240-247
CGTTG 4519570 0.7495313 5.115527 170-179
GCGGC 5467335 0.7407778 5.2899985 130-139
AGCGT 3826505 0.7344568 5.2318206 160-169
TGAGA 3162465 0.73344487 6.0878215 240-247
TAGGT 3643025 0.7300163 6.0187907 10-14
AGGAT 3115850 0.72263384 8.564477 10-14
ATACC 2453430 0.7175474 6.3336296 240-247
GTCGA 3721840 0.71436757 9.44876 70-79
CGAAC 2851760 0.7114033 9.897846 70-79
GGAAG 3586165 0.70941114 9.712912 30-34
GAAGT 2992805 0.6940969 5.056651 70-79
CAATC 2367120 0.6923045 9.890431 240-247
GCGTT 4110540 0.68169725 5.161892 170-179
GGTCC 4261635 0.6769593 8.730295 50-59
AATGG 2872595 0.6662177 5.531058 10-14
ACGTC 3069780 0.66166604 7.014991 70-79
ACGAT 2536410 0.6605851 7.9776735 10-14
TTTCG 3376475 0.6564932 5.12979 100-109
AAAGC 2169750 0.654019 8.960014 70-79
TGGAT 3223440 0.64593685 6.409722 10-14
GACCG 3397365 0.62459683 12.552219 45-49
CTATC 2467870 0.6236313 5.2363434 90-99
AAGGT 2618490 0.60728514 6.690933 10-14
AAGTC 2305185 0.6003647 5.0171638 70-79
ATAGG 2572580 0.5966376 5.3805466 10-14
GAAGC 2548275 0.5660849 8.806081 30-34
CGTCC 3154965 0.56279266 5.580372 70-79
CATCC 2308135 0.5586765 6.7813797 240-247
AAGCT 2078005 0.5411977 14.13377 30-34
TCAAG 2053035 0.53469455 5.9711313 70-79
TTGCC 2870605 0.5346058 5.973158 60-69
GACGA 2328730 0.51731426 7.01883 40-44
CGGTT 3011245 0.49938878 9.619888 10-14
GGGAT 2850060 0.48713624 5.98016 10-14
TAAAG 1523540 0.47944736 5.686196 9
AAGCA 1577285 0.4754347 5.6614385 70-79
TCGAT 1841355 0.4143578 6.1905994 10-14
GCGAT 1722185 0.33055505 5.104547 10-14