FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_15_SL254625.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_15_SL254625.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6530102
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7469950 8.166805 57.37435 110-119
CACAG 9716665 7.023131 40.006344 110-119
TCCCT 10344695 6.3791103 91.497215 30-34
CAGTA 8330320 6.370202 41.845417 60-69
ATACA 5947655 6.1461363 31.717054 110-119
GGAGA 10473805 6.0727153 90.97925 35-39
GGTGA 11289255 5.7216916 91.05795 45-49
AGTAA 6173095 5.7133636 42.70028 110-119
CCCTG 10480530 5.471169 54.621063 30-34
GTAAT 6698290 5.41918 37.01456 110-119
ACAGT 6413500 4.904409 32.985226 110-119
GGCCC 11079855 4.8964953 57.013805 60-69
AGACG 7541850 4.882293 98.23813 40-44
GTAGT 8007020 4.7937527 24.389688 70-79
GTGAC 8450250 4.781853 82.030556 45-49
GAGAC 7319305 4.738227 127.08285 40-44
CCCCA 7097375 4.732399 99.339874 60-69
GTGTC 8873720 4.38948 25.522501 120-129
ACCAG 5992775 4.3315315 94.69278 45-49
AATAC 4176620 4.3159995 31.550127 110-119
ACTCC 6052805 4.269907 91.28425 30-34
CGTGT 8557665 4.2331395 24.519989 120-129
GCACA 5839690 4.2208824 32.625458 110-119
CCCTT 6745350 4.1595554 64.4659 20-24
TGACC 6567270 4.1493406 149.58978 45-49
GACCA 5497965 3.9738865 105.54973 45-49
TGGTG 8960545 3.9698517 29.040636 25-29
TAGTA 4879670 3.9478447 25.316332 80-89
CCCAG 6574745 3.926405 82.41336 60-69
CCACT 5529370 3.9006534 27.57826 240-247
CTCAG 6105840 3.8577995 28.99626 140-149
CACTC 5451675 3.845844 78.05958 25-29
TGAGG 7562050 3.832646 69.31726 35-39
AGTAG 5500420 3.7672105 27.153973 70-79
CTCCC 6293045 3.667965 56.305065 30-34
CAGGG 7652675 3.666016 33.518192 50-59
CTTGG 7394000 3.6575208 37.602123 20-24
GACGG 7580295 3.6313424 110.05828 40-44
CTGAG 6383785 3.6124759 96.50597 35-39
AGGAG 6223395 3.6083267 90.61515 35-39
GCCCC 7186785 3.54612 73.23201 60-69
TGTCT 6037080 3.5276046 20.52985 170-179
ACGGT 6224500 3.5223389 92.02301 40-44
TCTTG 6013035 3.5135543 19.233208 170-179
ATGGT 5849295 3.5019362 24.78896 190-199
AGATA 3654450 3.3822906 18.239809 150-159
AGGCT 5944770 3.3640444 44.197575 30-34
GAGGA 5759385 3.3392932 126.69105 35-39
GCCCT 6324890 3.3017926 34.26753 20-24
TGTCC 5954965 3.288926 19.699915 120-129
CTTCA 4400845 3.2845497 16.799961 200-209
CCAGG 6132050 3.2798524 39.168423 45-49
TTCCC 5261740 3.2446787 56.05726 50-59
TCAGG 5661555 3.203778 19.61272 140-149
GGGTT 7227105 3.201874 43.04387 50-59
ATGCA 4174105 3.1919415 77.05259 25-29
GATGG 6268370 3.1769748 33.57242 15-19
CCTGG 6790255 3.174793 31.763756 50-59
CTCTC 5118475 3.1563344 13.885181 100-109
CCGTG 6673355 3.1201363 24.24343 120-129
ACAGA 3488750 3.0519733 14.680477 140-149
GGATG 5956960 3.019144 55.865124 20-24
TCGCA 4774555 3.016665 24.874811 100-109
CCAGT 4771700 3.014861 41.2436 60-69
GACTC 4744360 2.9975872 21.14806 190-199
GGTTC 6055075 2.9952073 48.61647 50-59
TGCAC 4713630 2.9781713 100.39124 25-29
CGCAC 4962140 2.963365 25.320414 100-109
TGGAG 5823450 2.9514778 31.537477 25-29
TCTCA 3911035 2.9189823 20.747694 130-139
TACAC 3418170 2.918453 22.973822 110-119
TCACA 3386685 2.8915713 12.629129 130-139
TCTCT 4403090 2.872619 15.324598 180-189
TGGCC 6137925 2.869795 38.7741 60-69
TTCTT 4086390 2.8205793 21.212223 170-179
GTTCC 5069835 2.8000686 55.398674 50-59
AAAAA 1950490 2.7907233 13.257366 230-239
AGGGT 5473245 2.7739847 50.481236 50-59
GCCGT 5926805 2.7710855 23.111462 120-129
CCTGA 4352785 2.750182 64.22095 30-34
CAGAT 3575160 2.7339277 15.387121 150-159
GGTTG 6162380 2.7301614 57.648357 15-19
GCACT 4315825 2.7268298 70.81557 25-29
CGGTG 6502835 2.723106 66.91166 40-44
CGGAT 4776635 2.7030165 63.10035 20-24
AGGGA 4650930 2.6966107 10.033844 150-159
ACCAC 3304285 2.666604 15.122935 230-239
TCAAA 2564630 2.6502151 54.652325 70-79
AATAA 2109595 2.6384742 13.014817 110-119
AGTCA 3438820 2.6296685 29.516294 70-79
CGGCC 5925540 2.6186602 11.128273 120-129
GGCTG 6136330 2.5696297 24.787985 30-34
AGATG 3641125 2.4937885 17.013134 180-189
GTTGG 5595670 2.479088 45.16686 15-19
CTGGC 5286320 2.471626 34.64199 60-69
TGTTC 4185630 2.4457598 12.950261 140-149
GATGC 4289000 2.4270723 73.1641 25-29
ACTCT 3218250 2.4019256 13.136629 200-209
GAGAT 3501895 2.3984306 16.677965 180-189
GTCTC 4241945 2.3428252 12.262052 180-189
TTGTC 3955520 2.3113008 15.427885 170-179
GTTCT 3954145 2.3104978 18.661793 160-169
CAGAG 3565075 2.3078878 9.648883 140-149
CATTT 2903870 2.292952 16.888083 150-159
TCATT 2897235 2.287713 16.255028 140-149
TTCAT 2875530 2.270574 17.738192 140-149
ACAGC 3125725 2.2592497 12.15374 110-119
CTGGA 3989965 2.2578537 15.165581 180-189
TCCTC 3656105 2.2545562 14.447041 130-139
TGTAG 3750175 2.2452064 9.1099 210-219
TCCAT 2977790 2.2224593 11.894262 70-79
ATATA 2031345 2.2208447 10.312539 110-119
TTGGT 4240910 2.2194455 41.763664 25-29
GTCAA 2885855 2.2068157 40.10058 70-79
GGCGG 6199525 2.1977317 35.87788 20-24
CACTA 2544310 2.1723466 14.251795 240-247
GTGAA 3162180 2.1657615 16.926285 190-199
TAGTC 3232460 2.1607542 24.689173 70-79
TTCAC 2894200 2.160072 17.264778 200-209
GCGGA 4465875 2.1393788 76.0481 20-24
TGAAT 2616670 2.116989 14.620992 190-199
TTGGA 3526130 2.1110718 17.019285 170-179
ATTTG 2975515 2.1043231 14.841459 150-159
AATAT 1915995 2.0947337 12.37672 110-119
GAATC 2739240 2.094699 15.105033 190-199
GAGTC 3658495 2.0702803 12.359222 210-219
CAGCC 3455740 2.0637505 10.935513 120-129
CTCTG 3736335 2.0635772 14.83491 180-189
TCTGC 3728630 2.0593214 10.780078 210-219
ACTAC 2399610 2.048801 12.018819 230-239
CCTTG 3695170 2.0408416 42.439014 20-24
TCTCG 3693205 2.0397565 16.6699 100-109
AATAG 2201615 2.0376532 8.375845 240-247
GATAT 2516520 2.0359635 9.629563 180-189
CTACT 2727430 2.0356045 8.488682 230-239
CTCGC 3898010 2.0348847 17.171371 100-109
GTTCA 3028295 2.024279 15.732259 140-149
ATAGA 2181795 2.0193093 10.3402195 240-247
GATAC 2637875 2.0171852 13.361087 150-159
ATATG 2489925 2.0144472 9.684625 180-189
CCTTC 3264145 2.0128517 13.596036 200-209
TCGGC 4292120 2.0067866 8.911243 190-199
TTGGG 4459240 1.9756075 71.34053 15-19
CACGG 3683795 1.9703531 7.592318 200-209
TCCCA 2753085 1.9421436 11.614897 240-247
GTTGT 3705385 1.939183 8.039344 210-219
ACACA 1983435 1.9372977 13.884544 110-119
AATCG 2530410 1.9350065 13.820556 190-199
GCTGA 3401805 1.9250237 38.57298 30-34
GTCAC 3028835 1.9136822 9.525891 130-139
GAACT 2500465 1.9121076 11.735132 160-169
GGTCA 3355205 1.8986537 10.1300335 130-139
TACAG 2480285 1.896676 11.296181 160-169
TTGCA 2833600 1.894134 13.370152 150-159
TGGAA 2720420 1.8632022 9.199185 25-29
GTCCT 3365305 1.8586571 12.347151 130-139
TACCA 2167830 1.8509057 11.54707 230-239
TTTGC 3165595 1.8497299 11.491364 150-159
ATTGT 2605115 1.8423715 8.381848 170-179
GCTGT 3722875 1.8415596 9.817112 140-149
AGTAT 2262690 1.8306051 11.642466 220-229
TACTA 1996990 1.8039023 10.743826 230-239
AGTCT 2689090 1.7975358 12.983764 210-219
CTGTT 3064245 1.7905087 12.477881 140-149
TTGTA 2525605 1.7861408 10.560156 210-219
TCAGC 2814855 1.7784851 11.382891 140-149
CATAG 2323260 1.7765988 8.290262 210-219
GTCTG 3579655 1.7707144 8.791569 210-219
CAGGC 3293630 1.7616652 13.473988 140-149
ATGTA 2155325 1.7437426 10.301411 70-79
AGAAC 1991180 1.7418926 11.261539 160-169
TGGGG 4621840 1.7334416 42.44174 15-19
TTCAG 2573605 1.7203393 10.549345 150-159
GTGGA 3387620 1.7169349 38.93467 25-29
ATAGT 2104540 1.7026556 7.051168 220-229
CACCA 2099255 1.694128 11.006335 230-239
TCTGG 3419645 1.6915638 12.284989 180-189
CTCCA 2388525 1.6849674 8.597074 240-247
CCCAC 2524320 1.6831703 10.618096 240-247
ATCTC 2242390 1.6735969 6.9521194 100-109
ACGGC 3113615 1.6653806 12.321525 120-129
CAGCT 2628225 1.6605682 8.9988575 150-159
GCAGA 2561335 1.6581062 13.50472 150-159
GGAGG 3855365 1.654166 26.853199 30-34
CCAGA 2282525 1.6497914 22.49124 60-69
TGACT 2464035 1.6470966 12.186537 190-199
GACGT 2909195 1.6462641 10.500755 170-179
GAGAA 2097580 1.6434703 11.147188 160-169
AACTG 2113200 1.6159657 11.052665 160-169
TGCAG 2852985 1.6144559 12.551676 150-159
CACTG 2553010 1.6130458 6.856378 230-239
GGACG 3356760 1.6080568 8.355685 170-179
CGACT 2543125 1.6068003 10.001909 190-199
ATAAT 1468845 1.6058701 5.5467463 110-119
GGGGC 4499560 1.5950941 39.168827 15-19
TGGTT 3018125 1.579511 16.230886 10-14
AACCA 1616740 1.5791323 12.175846 70-79
ACTGA 2058445 1.5740947 7.839643 180-189
CTCTT 2408335 1.5712212 9.339806 200-209
ATAAA 1252305 1.5662601 6.509262 110-119
TCGAC 2476465 1.5646831 10.626516 190-199
CAAAG 1786500 1.5628377 28.542595 70-79
GTCCC 2940800 1.5351906 16.286102 50-59
ATGGG 3024990 1.5331446 53.75997 15-19
ACGGA 2366765 1.5321493 10.11872 200-209
GGGCG 4317440 1.5305325 46.158768 20-24
CAAAA 1280925 1.5142564 19.201674 70-79
AGCTC 2378025 1.5024866 9.620337 140-149
ACACG 2073115 1.4984316 9.437259 110-119
CCTCG 2846730 1.486083 9.02614 130-139
CTGGT 2972225 1.4702427 7.380036 160-169
TCAGA 1915465 1.4647577 8.76624 130-139
GAGGG 3411210 1.4635988 9.773314 200-209
CTCGA 2311415 1.4604012 9.505065 190-199
TCCAC 2069425 1.4598608 10.439001 240-247
ATCGG 2578330 1.4590333 9.81718 190-199
TGGAC 2576685 1.4581023 11.34373 170-179
AAATA 1165730 1.4579805 5.7848196 6
GCTCT 2611590 1.4423804 9.149617 130-139
CATGT 2150450 1.4374794 9.776411 70-79
GGCCG 3623125 1.4340593 11.96328 120-129
CCACC 2150685 1.4340373 8.25042 230-239
AATTG 1759050 1.4231405 9.263948 170-179
CTTGA 2125365 1.420711 9.791122 200-209
AGCCG 2639055 1.4115525 10.761854 120-129
CCATC 2000330 1.411118 10.592657 240-247
GGGAG 3257290 1.3975588 7.158071 150-159
TAGTT 1973115 1.3954128 8.559675 220-229
GAATT 1678035 1.3575963 10.302228 170-179
CGTAG 2396740 1.3562745 8.150151 210-219
CAATA 1299480 1.3428454 7.3013887 100-109
CAGAC 1846570 1.3346865 11.50595 60-69
GAGGC 2781130 1.332301 23.61111 30-34
ACTCG 2106490 1.330925 9.86923 190-199
TATGG 2221155 1.3297915 6.8766503 180-189
ATCCA 1555695 1.3282613 16.46146 240-247
ACTAT 1462615 1.3211956 7.0731945 230-239
TATAC 1462335 1.3209426 6.456407 110-119
ACTGT 1973195 1.3189924 5.5303197 230-239
ACGTG 2325385 1.315896 10.136387 170-179
ACCAT 1518335 1.2963632 9.394915 45-49
GTGCT 2615565 1.293817 7.566162 220-229
GGCTC 2762705 1.2917064 8.196703 130-139
GGTGG 3412055 1.2797064 39.149097 25-29
TTCCA 1710110 1.2763323 7.45959 230-239
AAAGT 1368100 1.2662128 21.23021 70-79
TGATA 1561610 1.2634039 7.2143526 180-189
GGGCC 3187670 1.261703 30.229118 15-19
AAGTA 1354890 1.2539867 17.128523 70-79
ACTTC 1680145 1.2539682 5.545734 220-229
GTGTT 2393190 1.2524564 7.258843 160-169
GGAAT 1827075 1.2513547 8.328107 170-179
GCTCA 1971055 1.2453542 9.479549 140-149
TTGAG 2059150 1.2328001 8.896349 200-209
CCATG 1947560 1.2305096 9.74826 70-79
TGGGC 2927820 1.2260443 32.417507 15-19
AGAGA 1562030 1.2238626 11.958432 35-39
GGAGT 2403200 1.2180051 6.8975444 210-219
CCTCA 1725265 1.2170753 5.5176783 130-139
TGTGC 2450835 1.2123317 6.1453032 220-229
GTCCA 1915470 1.2102345 11.103433 70-79
CTGAT 1798370 1.2021296 6.3230357 180-189
GAACC 1658475 1.1987329 8.882753 70-79
CTGAA 1558305 1.1916372 13.432312 35-39
GCGTA 2083155 1.178822 9.160812 210-219
TATCA 1295620 1.1703473 5.4849787 70-79
ACTGG 2062995 1.167414 7.4972296 160-169
AGTTC 1729090 1.1558189 5.818658 160-169
GTCTA 1719150 1.1491746 7.694036 180-189
TCACG 1812690 1.145296 8.721262 200-209
CTGCG 2429260 1.1358038 6.2743907 210-219
AGATC 1476495 1.1290768 5.4658656 130-139
TAGAT 1391195 1.1255314 8.337651 240-247
GCATA 1467630 1.1222978 7.694618 210-219
TGCCC 2145700 1.1201233 9.449438 60-69
GAAGA 1411160 1.1056548 17.40557 35-39
TATGT 1557495 1.1014808 7.4402566 220-229
ACAAT 1062145 1.0975902 8.392609 100-109
ATCAT 1214615 1.0971745 5.1935635 90-99
AGGTT 1826595 1.093571 18.050245 10-14
AGAGC 1682915 1.0894523 6.765996 140-149
CCCAA 1347210 1.0872172 9.736482 240-247
TCTAC 1455230 1.086104 7.292305 180-189
GCGGT 2588105 1.0837864 7.3882914 130-139
CCAAT 1245690 1.0635773 10.954362 240-247
CGGCT 2251310 1.052603 7.34176 130-139
TATAA 958490 1.0479053 7.339191 240-247
AAGAG 1334315 1.0454462 11.763401 35-39
AAAAG 985730 1.0436772 12.373974 70-79
CTCGG 2232105 1.0436238 7.427747 130-139
TCCAG 1634580 1.0327624 5.6165276 240-247
ATAAC 999180 1.032524 7.08168 80-89
GTGGT 2324625 1.0298946 7.130899 50-59
TGGTC 2078740 1.0282708 8.093125 50-59
CGGTC 2176355 1.0175576 7.521295 130-139
TGTAA 1239095 1.0024766 6.601591 230-239
TACTG 1497030 1.0006973 5.3523116 180-189
ACCCA 1234945 0.9966178 11.349657 240-247
ATATC 1098280 0.99208796 5.6516123 70-79
GCCAC 1657700 0.9899701 5.0293617 80-89
TCTGT 1694060 0.9898782 5.2640915 210-219
CATCA 1153595 0.9849461 5.780653 230-239
CGATG 1737200 0.9830521 23.360565 15-19
CGGAG 2016580 0.9660432 6.4568095 210-219
AGCTT 1444425 0.9655332 5.7370605 150-159
GCTTC 1734590 0.9580136 5.4469285 150-159
ACTAA 919270 0.9499473 11.009892 240-247
GAGTT 1574825 0.94283783 5.222208 160-169
TAGAC 1216855 0.93052995 5.027967 110-119
ACCGT 1469070 0.9281896 12.989186 45-49
CCAGC 1544700 0.9224871 5.9820824 80-89
CAATC 1069955 0.91353375 10.742819 240-247
CTTCC 1475490 0.9098686 5.1117396 230-239
TGAAG 1311460 0.8982125 9.618673 35-39
CTACC 1249700 0.8815917 6.368891 230-239
TGCAT 1302520 0.87067616 6.345943 210-219
AATCC 1014905 0.86653167 10.488703 240-247
TGAAC 1123700 0.8592943 5.2315207 190-199
GAGCT 1518340 0.8592028 5.588356 140-149
AGCTG 1504870 0.85158044 7.34104 30-34
ATCAA 819385 0.846729 12.148024 70-79
AGACC 1155875 0.8354575 5.5836873 240-247
TGCGT 1684480 0.83324593 6.813487 210-219
GGAAG 1418720 0.8225744 10.479076 30-34
AGCCA 1122945 0.81165594 7.643053 80-89
GTATG 1355420 0.8114815 5.0173726 220-229
CCTTT 1234275 0.80525297 6.1152244 50-59
ACGTC 1243960 0.7859603 7.578781 70-79
AAAGG 988405 0.774423 7.7549706 10-14
TGAGA 1119550 0.7667743 6.2670026 240-247
TAACC 879255 0.750713 6.1676073 80-89
CGGCG 1874155 0.7418041 5.2407036 130-139
TCGAA 968020 0.7402457 12.95849 70-79
ATACC 866535 0.7398526 6.2650957 240-247
CTAAT 817955 0.73886746 8.734664 240-247
CGTTG 1491820 0.7379446 5.0235114 170-179
TAGGT 1212870 0.72613764 5.6662602 10-14
GCGGC 1822540 0.7213746 5.246228 130-139
AGGAT 1050275 0.71932817 8.351334 10-14
TGGAT 1196780 0.7165047 7.1381927 10-14
GACCG 1321120 0.7066281 13.427161 45-49
GTCGA 1228340 0.6950968 8.839213 70-79
ACGAT 906175 0.69295275 8.71866 10-14
GGTCC 1474460 0.6893858 9.160725 50-59
AATGG 972640 0.6661563 5.644904 10-14
CGAAC 917525 0.66317993 8.899979 70-79
AAGGT 956650 0.6552049 6.881926 10-14
CTTTG 1086345 0.63477635 5.3179417 50-59
CATCC 893100 0.6300308 6.91316 240-247
AAAGC 704315 0.6161377 8.587026 70-79
GAAGC 924020 0.5981738 9.7853 30-34
CGTCC 1126025 0.5878206 5.976874 70-79
ATAGG 857155 0.5870612 5.5468087 10-14
TTGCC 1045190 0.57725817 6.401564 60-69
AAGCT 734800 0.56190217 15.145403 30-34
TCAAG 720305 0.55081785 5.8168836 70-79
GACGA 783650 0.5073038 6.4003983 40-44
GGGAT 959050 0.48607183 6.1299725 10-14
TAAAG 517685 0.47913122 5.155016 9
CGGTT 957295 0.47353613 9.513309 10-14
AAGCA 509290 0.445529 5.1916637 70-79
TCGAT 649335 0.4340513 6.7934933 10-14
GCGAT 594855 0.33661833 5.277891 10-14