FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_14_SL254624.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_14_SL254624.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4993962
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 5441255 7.6723237 53.511475 110-119
CACAG 7611360 7.0649924 37.94391 110-119
TCCCT 7963375 6.3872795 92.63555 30-34
CAGTA 6196705 6.1399245 40.994728 60-69
GGAGA 7833190 5.9591174 88.40513 35-39
ATACA 4510760 5.9583325 30.037586 110-119
GGTGA 8433745 5.6747518 89.85934 45-49
AGTAA 4600105 5.500975 39.949192 110-119
CCCTG 8066055 5.486862 54.768173 30-34
GTAAT 4914755 5.1982474 35.100685 110-119
ACAGT 5112590 5.0657434 31.862946 110-119
GTAGT 5999565 4.7599463 24.4187 70-79
GGCCC 8208535 4.735574 55.22759 60-69
GAGAC 5615305 4.7186713 121.5528 40-44
AAAAA 2603005 4.691768 29.864254 230-239
AGACG 5549535 4.663403 93.59052 40-44
GTGAC 6252430 4.6470604 79.63898 45-49
CCCCA 5388185 4.5774684 93.10026 60-69
ACTCC 5007140 4.5407267 95.786514 30-34
GTGTC 6517880 4.284685 24.587088 120-129
ACCAG 4587090 4.2578144 90.57938 45-49
GCACA 4543745 4.2175803 31.171858 110-119
CGTGT 6311005 4.148691 23.845968 120-129
AATAC 3100965 4.0961123 30.0415 110-119
CACTC 4461875 4.046253 81.264305 25-29
CCCTT 4991250 4.0033917 59.02056 20-24
TGACC 4848730 3.9807103 143.47504 45-49
TGGTG 6607390 3.9322348 26.755651 25-29
TAGTA 3693265 3.9062994 24.483423 80-89
CTCAG 4696810 3.8559868 28.837042 140-149
GACCA 4142565 3.8451984 100.66092 45-49
CCCAG 4992330 3.8395739 78.075035 60-69
CCACT 4226195 3.8325262 26.467945 240-247
TGAGG 5662610 3.810159 68.75527 35-39
AGTAG 4135470 3.7095737 26.815136 70-79
CTCCC 4886470 3.6716492 57.65723 30-34
CAGGG 5770705 3.6374953 33.190945 50-59
AGGAG 4678620 3.559271 87.88587 35-39
CTGAG 4784145 3.555771 94.61074 35-39
CTTGG 5408265 3.5552533 35.69525 20-24
TGTCT 4525790 3.508027 20.294867 170-179
GACGG 5558825 3.5039392 106.98508 40-44
ATGGT 4379960 3.474981 24.30108 190-199
TCTTG 4474645 3.4683826 18.983461 170-179
CTTCA 3551905 3.4383476 16.723553 200-209
ACGGT 4603305 3.4213634 89.35581 40-44
GCCCC 5332950 3.3984244 69.58581 60-69
AGATA 2805380 3.3547769 17.532618 150-159
ATGCA 3367670 3.3368123 81.06256 25-29
AGGCT 4432185 3.2941802 40.896038 30-34
GGGTT 5488120 3.266127 43.35533 50-59
GAGGA 4287310 3.261581 122.634995 35-39
CCTGG 5241085 3.2276094 31.438715 50-59
TGTCC 4442545 3.225875 19.070702 120-129
TTCCC 3997655 3.206447 54.802406 50-59
CCAGG 4600110 3.2029107 37.83633 50-59
GCCCT 4658040 3.1685903 30.666126 20-24
TCAGG 4262175 3.167822 19.782314 140-149
GGATG 4706375 3.1667435 59.905678 20-24
CTCTC 3897970 3.1264913 13.190113 100-109
GATGG 4617635 3.1070337 30.660147 15-19
CCAGT 3784240 3.106785 40.10066 60-69
TGCAC 3780690 3.1038709 104.93326 25-29
CCGTG 4921940 3.0310705 23.334549 120-129
GGTTC 4576620 3.008551 48.40956 50-59
GTTCC 4116915 2.9894245 54.517597 50-59
TCACA 2699240 2.9542475 11.71014 130-139
TGGAG 4341790 2.9214284 30.038319 25-29
ACAGA 2606200 2.9196234 13.576171 140-149
TCGCA 3545410 2.9107106 23.53906 100-109
TCTCA 2998115 2.9022627 20.047485 130-139
TACAC 2614440 2.8614361 21.975925 110-119
GCACT 3462025 2.842253 74.44448 25-29
GGTTG 4761930 2.833952 63.17825 15-19
TCTCT 3304645 2.829413 14.989524 180-189
TTCTT 3094055 2.8278239 21.17617 170-179
CGGAT 3802235 2.8259757 67.13854 20-24
CGCAC 3673210 2.825046 23.86154 100-109
GACTC 3434955 2.8200293 20.222618 190-199
TGGCC 4560830 2.8086894 38.096775 60-69
AGGGT 4153990 2.7950642 50.208656 50-59
GCCGT 4527025 2.787871 22.197824 120-129
CAGAT 2788200 2.762652 14.949351 150-159
AGTCA 2760370 2.7350767 28.678183 70-79
CCTGA 3319400 2.7251608 64.1123 30-34
ACCAC 2613010 2.6791291 14.479099 230-239
CGGTG 4774115 2.6616414 65.93043 40-44
GTTGG 4428900 2.635757 49.152454 15-19
AGGGA 3447140 2.6224198 9.512712 150-159
GATGC 3435110 2.5531135 78.29315 25-29
GGCTG 4559200 2.541823 23.082716 30-34
CGGCC 4397165 2.536762 10.714547 120-129
TCAAA 1892495 2.499826 51.30331 70-79
TCCAT 2581685 2.4991465 11.624801 70-79
AATAA 1544845 2.4628026 11.301341 110-119
TGTTC 3159350 2.448873 13.055882 140-149
AGATG 2719010 2.4389896 16.532827 180-189
CTGGC 3952230 2.433896 34.171307 60-69
GAGAT 2656235 2.3826795 16.14061 180-189
GTCTC 3256610 2.3647292 12.195471 180-189
ACTCT 2397945 2.3212805 12.527438 190-199
GTTCT 2985920 2.314444 18.481058 160-169
TCCTC 2881100 2.3108783 14.430405 130-139
GGCGG 4853710 2.2949574 38.843685 20-24
TTGTC 2937690 2.2770598 14.969893 170-179
TTCAT 2200310 2.2736578 17.155712 140-149
CAGAG 2704480 2.2726374 9.193649 140-149
GCGGA 3600855 2.2697563 80.51146 20-24
TCATT 2191515 2.2645698 15.671963 140-149
CTGGA 3044405 2.2627256 14.537806 180-189
CATTT 2189285 2.2622654 16.831932 150-159
TTCAC 2293075 2.2197635 16.793503 200-209
ACAGC 2361630 2.1921046 11.349015 120-129
TGTAG 2751530 2.1830142 8.6848755 210-219
TTGGT 3085890 2.1654382 38.39992 25-29
GTGAA 2390390 2.144213 16.16442 190-199
TCTGC 2950540 2.1424818 10.833704 210-219
TTGGG 3568200 2.123531 77.016525 15-19
GATAT 2005750 2.1214454 9.54083 180-189
TAGTC 2416390 2.1176412 24.813992 70-79
CACTA 1931855 2.1143646 13.813086 240-247
ATATA 1490405 2.1015131 9.743528 110-119
ATTTG 2234475 2.0903242 14.822974 150-159
TTGGA 2634615 2.0902555 16.416174 170-179
TGAAT 1975565 2.0895195 14.040309 190-199
GAGTC 2804800 2.0846415 12.205143 210-219
CTCTG 2867145 2.081926 14.489449 180-189
TCTCG 2861195 2.0776055 16.005138 100-109
ACTAC 1885010 2.063094 12.379238 230-239
GTTCA 2352365 2.061532 15.550009 140-149
GATAC 2062050 2.0431557 12.844515 150-159
CTACT 2100825 2.03366 8.473418 230-239
CCTTC 2535340 2.0335505 13.228135 200-209
GAATC 2038945 2.0202622 14.310672 190-199
GAACT 2034490 2.0158482 12.098224 160-169
AATAT 1423515 2.0071962 11.457687 110-119
TCGGC 3250945 2.0020247 8.469831 190-199
CAGCC 2599160 1.9989998 10.421775 120-129
GTCAC 2399945 1.9703069 8.974069 130-139
ATATG 1858925 1.9661512 9.398954 180-189
CTCGC 2866255 1.9497445 16.279873 100-109
GTCAA 1949350 1.9314883 38.235947 70-79
GTCTG 2932660 1.9278547 8.730162 210-219
CCTTG 2636740 1.9146216 39.872036 20-24
CACGG 2735250 1.9044678 7.368814 200-209
ACACA 1536660 1.9015161 12.716045 110-119
GTTGT 2701445 1.8956646 7.630979 210-219
AATAG 1580785 1.8903608 8.209996 240-247
TACAG 1906570 1.8891002 11.349184 160-169
TCAGC 2293840 1.8831967 11.240733 140-149
TGGGG 3730865 1.8830557 46.1783 15-19
TTGCA 2143130 1.8781655 13.116505 150-159
ATAGA 1564835 1.8712873 9.397146 240-247
TCCCA 2063105 1.8709277 10.753597 240-247
CTCCA 2062805 1.8706557 8.285189 240-247
AATCG 1887785 1.8704872 13.160925 190-199
GCTGT 2803560 1.8429877 9.856984 140-149
GTCCT 2537970 1.8429016 12.28684 130-139
TACTA 1577015 1.8424426 10.629682 230-239
TTTGC 2372945 1.8393152 11.536442 150-159
TACCA 1676730 1.835137 11.40086 230-239
AGTAT 1730820 1.8306569 11.31347 220-229
AGTCT 2084650 1.8269156 12.550567 210-219
TTCAG 2081600 1.8242427 9.944099 150-159
ATTGT 1947070 1.8214604 8.5127535 170-179
CACCA 1755880 1.8003104 10.030216 230-239
GGTCA 2420985 1.7993745 9.419971 130-139
GCTGA 2399250 1.7832202 35.174908 30-34
TGGAA 1984210 1.779864 8.741246 170-179
CTGTT 2289405 1.7745616 12.401607 140-149
CATAG 1781335 1.7650126 7.6679955 210-219
CAGGC 2533395 1.7639228 13.275406 140-149
ATCTC 1811410 1.7534978 6.545968 100-109
CCAGA 1861140 1.7275414 20.625076 60-69
GGGGC 3640155 1.7211576 42.259377 15-19
TTGTA 1830235 1.7121626 9.847233 210-219
ATAGT 1611305 1.7042481 6.678404 220-229
ATGTA 1600335 1.6926453 9.728749 70-79
CAGCT 2059130 1.6905044 9.016725 150-159
CCATC 1862325 1.6888502 11.050091 240-247
TCTGG 2567845 1.6880348 11.936268 180-189
CCCAC 1951775 1.6581072 9.937721 240-247
GGGCG 3466585 1.6390893 50.21992 20-24
GCAGA 1937550 1.6281682 12.984311 150-159
TGCAG 2189910 1.6276301 12.351877 150-159
GTGGA 2412345 1.6231769 36.96022 25-29
GGAGG 2833690 1.6170477 24.99472 30-34
AGAAC 1441215 1.6145366 10.289042 160-169
ACACG 1735985 1.6113706 9.308854 110-119
TGGTT 2284625 1.6031727 17.33683 10-14
ACGGC 2291895 1.595774 11.8977585 120-129
ATCGG 2146440 1.5953215 9.434409 190-199
TGACT 1815020 1.5906212 11.818429 190-199
TTCCA 1639375 1.5869628 7.2431855 230-239
CAAAG 1393345 1.5609097 26.809406 70-79
CACTG 1892090 1.553368 6.550511 230-239
GACGT 2087745 1.551697 9.540838 170-179
GAGAA 1528945 1.550629 10.1062565 160-169
CTCTT 1799170 1.5404363 9.007827 200-209
ACTGA 1550390 1.5361838 7.2933764 180-189
AACTG 1545720 1.5315566 10.029918 160-169
AGCTC 1854185 1.5222486 9.454994 140-149
GGACG 2406025 1.5166093 7.5406694 170-179
AACCA 1211570 1.4992385 11.151124 70-79
CGACT 1821915 1.4957557 9.362873 190-199
ATAAA 936100 1.4923371 6.8221583 8
CTGGT 2264825 1.4888372 7.0219 160-169
GTCCC 2187975 1.4883505 15.488672 50-59
ATAAT 1053375 1.4852885 5.2037816 110-119
GCTCT 2043760 1.4840398 9.272642 130-139
CCACC 1744780 1.4822569 7.686996 230-239
CTTGA 1690145 1.481185 9.39099 200-209
ATGGG 2184240 1.4696935 50.42673 15-19
TCGAC 1783055 1.4638525 9.828397 190-199
AGTTC 1666745 1.460678 6.471967 160-169
ACGGA 1737185 1.4597969 9.588946 200-209
CCTCG 2143880 1.4583553 8.821216 130-139
GAGGG 2553980 1.4574308 9.383756 200-209
CAAAA 972680 1.4526532 17.742155 70-79
TCCAC 1579770 1.4326152 10.243621 240-247
TGGAC 1925135 1.4308386 10.437874 170-179
AAATA 887465 1.4148029 5.570314 6
TCAGA 1427125 1.4140483 8.390475 130-139
TAGTT 1502415 1.405491 8.538263 220-229
CGTAG 1888455 1.4035765 8.140496 210-219
GGCCG 2674610 1.3968968 11.55525 120-129
AATTG 1309095 1.3846059 9.160769 170-179
AGCCG 1972250 1.3732152 10.044465 120-129
GGAAT 1522320 1.365542 8.560641 170-179
GGGAG 2389630 1.3636444 6.7409363 150-159
CTCGA 1658550 1.3616363 8.844207 190-199
CTGAA 1359730 1.3472708 12.224121 35-39
GGCTC 2184285 1.3451449 8.399101 130-139
ATCCA 1226610 1.3424925 15.685737 240-247
TATGG 1670515 1.3253564 6.865173 180-189
GAGGC 2099755 1.3235556 21.869131 30-34
CAGAC 1414175 1.312661 10.55352 60-69
GAATT 1228700 1.2995737 10.141529 170-179
GAAGA 1274950 1.2930318 15.923991 35-39
GTGTT 1840300 1.2913798 7.5294228 160-169
AGATC 1300230 1.2883161 5.983476 130-139
GTGCT 1959230 1.2879469 7.609745 220-229
ACTAT 1096230 1.2807364 6.5574803 230-239
CAATA 967850 1.2784479 6.7758055 100-109
CATGT 1455765 1.2757823 9.443782 70-79
TATAC 1077755 1.259152 6.027099 110-119
TCCAG 1530965 1.2568916 5.673429 240-247
ACTTC 1288915 1.2477074 5.319205 210-219
TGATA 1178430 1.246404 7.07407 180-189
ACCAT 1133585 1.2406789 9.133445 45-49
ACGTG 1668730 1.240268 9.127619 170-179
AAAGT 1037075 1.2401724 20.333925 70-79
ACTCG 1503295 1.2341751 9.039848 190-199
GGTGG 2444970 1.2340341 37.170307 25-29
ACTGT 1406290 1.2324243 5.243563 230-239
AAGTA 1028230 1.2295952 16.320473 70-79
GCTCA 1486530 1.2204113 9.015682 140-149
AGAGC 1447670 1.2165108 7.2294745 140-149
TGTGC 1837595 1.2079873 6.097977 220-229
AGAGA 1190135 1.207014 11.319503 35-39
GGAGT 1791055 1.2051338 6.891264 210-219
CCATG 1462770 1.2009048 9.329264 70-79
GCGTA 1612835 1.1987245 9.061959 210-219
TTGAG 1506370 1.1951265 8.441087 200-209
GGGCC 2285290 1.1935625 28.492027 15-19
CTGAT 1357570 1.1897278 6.148985 180-189
CCTCA 1310250 1.1882008 5.612386 130-139
AAGAG 1165240 1.1817658 11.239107 35-39
GTCCA 1434850 1.177983 10.604509 70-79
TATCA 996670 1.1644197 5.6779804 70-79
TGGGC 2085180 1.1625196 30.84316 15-19
GAACC 1251670 1.1618212 8.312466 70-79
TAGAT 1085130 1.1477224 7.73629 240-247
CTGCG 1853865 1.1416628 6.387923 210-219
ACTGG 1519305 1.1292093 6.944088 160-169
CCCAA 1089235 1.1167967 9.557965 240-247
TCACG 1354235 1.1117997 8.515973 200-209
AGGTT 1397110 1.1084417 19.145096 10-14
TATGT 1181495 1.1052742 7.2747216 220-229
ATATC 942015 1.1005657 5.5566597 70-79
GTCTA 1252910 1.0980072 7.1748257 180-189
GCATA 1104655 1.0945331 6.9987655 210-219
ACCCA 1053870 1.0805368 11.338857 240-247
TGCCC 1583685 1.0772876 8.704946 60-69
AAAAG 788515 1.0661019 12.009414 70-79
CGGCT 1715575 1.0565 7.3627434 130-139
GCGGT 1887160 1.0521203 6.858437 130-139
TGAAC 1058095 1.0483998 5.493145 190-199
CTCGG 1692270 1.0421481 7.317714 130-139
ACAAT 787635 1.0403992 7.7122726 100-109
CCAAT 949405 1.0390987 10.551656 240-247
TCTAC 1065900 1.0318223 6.811269 180-189
TGGTC 1539395 1.0119584 7.9282436 50-59
GTGGT 1695985 1.0093262 7.0428166 50-59
TCTGT 1291470 1.0010431 5.0579824 210-219
ATAAC 753660 0.9955212 6.383971 80-89
TACTG 1131810 0.99187946 5.0915365 180-189
AGACC 1064480 0.9880683 5.5563545 240-247
CCAGC 1274570 0.98026484 5.7181363 80-89
TATAA 692525 0.9764798 6.632166 240-247
GCTTC 1334625 0.96911407 5.3978434 150-159
CGGAG 1535060 0.96760684 6.407857 210-219
AGCTT 1096995 0.9613687 5.733492 150-159
CATCA 871095 0.95339054 5.3324223 230-239
CGGTC 1547035 0.95270824 6.8581414 130-139
TGTAA 895370 0.94701666 6.573885 230-239
GTATG 1188925 0.9432716 5.286577 220-229
ACTAA 709010 0.9365422 11.332283 240-247
GGAAG 1222670 0.930149 8.641496 30-34
GAGTT 1170850 0.9289311 5.252304 160-169
CGATG 1207260 0.89728475 20.178616 15-19
ACGTC 1088640 0.8937516 6.949177 70-79
ACCGT 1081245 0.8876805 12.145423 45-49
TGAAG 985220 0.88375604 8.64635 35-39
CTACC 972940 0.88231105 6.532189 230-239
GAGCT 1163545 0.86479396 5.43618 140-149
AATCC 786885 0.86122495 9.951713 240-247
TGCAT 980565 0.8593335 5.7872906 210-219
TGCGT 1288675 0.8471415 6.968442 210-219
AGCTG 1129425 0.83943456 6.5503397 30-34
ATCAA 627910 0.82941604 11.854645 70-79
AAAGG 787615 0.7987852 7.253341 10-14
AGCCA 855840 0.79440504 7.0067573 80-89
CCTTT 926470 0.79323685 5.874798 50-59
GACCG 1128545 0.7857701 12.310108 45-49
TGAGA 844945 0.7579274 5.8971415 240-247
TGGAT 952825 0.7559541 6.6410217 10-14
TAACC 672520 0.7360555 6.071415 80-89
CTAAT 625760 0.7310817 9.158473 240-247
TAGGT 900820 0.71469426 6.4041643 10-14
CAATC 651395 0.7129347 9.830575 240-247
AGGAT 785790 0.70486444 7.9173675 10-14
ATACC 630885 0.6904871 5.775079 240-247
TCGAA 687715 0.68141353 11.862359 70-79
GGTCC 1093200 0.6732237 8.721533 50-59
AAGGT 746915 0.66999304 6.8654547 10-14
AATGG 728400 0.65338486 5.4792953 10-14
GTCGA 873375 0.64912784 8.226534 70-79
CATCC 695370 0.6305966 7.2685957 240-247
ACGAT 636415 0.6305836 8.39365 10-14
CGAAC 676365 0.6278134 8.3153305 70-79
CTTTG 802215 0.62181216 5.164705 50-59
ATAGG 670235 0.60121006 5.764812 10-14
AAAGC 527565 0.59101033 7.8575354 70-79
GAAGC 668815 0.56202084 7.731737 30-34
CGTCC 818295 0.5566379 5.5513554 70-79
TTGCC 753650 0.5472495 5.8139167 60-69
TCAAG 539870 0.5349232 5.3523083 70-79
AAGCT 515515 0.51079136 12.803612 30-34
CGGTT 763555 0.5019412 10.523505 10-14
GACGA 584745 0.4913748 5.911118 40-44
GGGAT 711770 0.47892338 5.7446966 10-14
TAAAG 389810 0.46614918 5.7803245 9
AAGCA 411540 0.4610321 5.1034904 70-79
TCGAT 481980 0.42239073 6.5689225 10-14