FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_9_SL254636.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_9_SL254636.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10157281
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11774375 8.332003 58.458107 110-119
CACAG 15101760 7.085691 40.68428 110-119
CAGTA 13044500 6.448533 42.55267 60-69
TCCCT 16284585 6.41141 91.815346 30-34
ATACA 9433115 6.335601 31.447844 110-119
GGAGA 16364195 6.1490417 93.83166 35-39
AGTAA 9719505 5.841924 43.473396 110-119
GGTGA 17854520 5.8138204 92.35684 45-49
CCCTG 16606990 5.5535145 55.11401 30-34
GTAAT 10504105 5.4710593 37.50794 110-119
AGACG 11946655 5.01626 100.30827 40-44
GGCCC 17622440 5.005452 58.597282 60-69
ACAGT 10099900 4.9928737 34.17822 110-119
GTGAC 13414990 4.8811784 82.963 45-49
GAGAC 11463175 4.813253 130.02718 40-44
GTAGT 12512150 4.7967286 24.331545 70-79
CCCCA 11116690 4.7937217 101.92908 60-69
AATAC 6620185 4.4463415 31.792892 110-119
ACCAG 9416195 4.4180446 98.95496 45-49
GTGTC 13995160 4.4127855 25.301111 120-129
CGTGT 13431710 4.235125 24.380539 120-129
TGACC 10414640 4.2344747 152.45848 45-49
GCACA 8972860 4.2100334 34.022324 110-119
CCCTT 10614780 4.179148 65.06443 20-24
ACTCC 9152845 4.1584578 90.55601 30-34
GACCA 8751090 4.10598 110.69699 45-49
TAGTA 7722095 4.0220504 25.971292 80-89
TGGTG 14111015 3.9817348 28.623169 25-29
CTCAG 9725145 3.9541337 29.790848 140-149
CCACT 8685350 3.9460583 27.624283 240-247
CCCAG 10225195 3.9459174 84.105675 60-69
AGTAG 8722980 3.8590243 27.788895 70-79
CACTC 8401115 3.8169203 77.8598 25-29
TGAGG 11685370 3.8050108 69.77907 35-39
GACGG 12018695 3.7144275 113.03979 40-44
CTTGG 11658450 3.6760023 38.338753 20-24
AGGAG 9763225 3.668649 93.31638 35-39
CTCCC 9784570 3.6562822 56.395973 30-34
CTGAG 10024665 3.6475751 97.525986 35-39
CAGGG 11786690 3.6427252 34.319138 50-59
ACGGT 9871700 3.5919168 94.67765 40-44
GCCCC 11266475 3.5759068 74.79874 60-69
ATGGT 9320065 3.5729928 25.36654 190-199
AGGCT 9620675 3.5005789 45.656246 30-34
TGTCT 9415295 3.495173 20.706278 180-189
TCTTG 9346850 3.4697647 19.319216 170-179
AGATA 5709840 3.4319086 18.804354 150-159
GAGGA 9076990 3.4107873 129.82153 35-39
GCCCT 10162510 3.3984272 34.001217 20-24
CCAGG 9706805 3.352214 40.545525 45-49
TGTCC 9501435 3.347689 19.28537 120-129
TTCCC 8219290 3.2360191 56.5072 50-59
CTTCA 6750215 3.2312655 16.87685 200-209
TCAGG 8867620 3.2265728 19.971266 140-149
CCTGG 10766260 3.2219658 32.4777 50-59
GATGG 9880120 3.2171822 33.381325 15-19
CTCTC 8152765 3.2098277 13.9566145 100-109
ATGCA 6486000 3.2063468 76.846466 25-29
GGGTT 11206385 3.162129 43.628025 50-59
CCGTG 10556495 3.1591904 24.511683 120-129
ACAGA 5396280 3.0784166 15.020303 140-149
TCGCA 7524930 3.0595512 24.800726 100-109
CGCAC 7858325 3.032539 25.504797 100-109
GGATG 9260925 3.0155587 55.533524 20-24
TGGAG 9223510 3.0033755 32.366283 25-29
TCTCA 6253080 2.9932916 21.462416 130-139
GGTTC 9473930 2.9872055 49.30865 50-59
CCAGT 7290430 2.9642057 42.085926 60-69
GACTC 7272675 2.956987 21.098545 190-199
TGCAC 7246680 2.946418 99.89575 25-29
TACAC 5330230 2.9444242 22.465279 110-119
TCTCT 7084400 2.9387236 15.687215 180-189
TGGCC 9793225 2.9307706 39.22375 60-69
CAGAT 5837080 2.8855536 15.851225 150-159
GTTCC 8077590 2.8460186 56.24021 50-59
AGGGT 8640565 2.813556 51.818306 50-59
TTCTT 6363095 2.7810125 21.229729 170-179
TCAAA 4129550 2.7735462 57.46503 70-79
CCTGA 6821475 2.773534 65.15539 30-34
CGGTG 10322185 2.7644377 68.53554 40-44
GCCGT 9218000 2.7586257 23.262997 120-129
AATAA 3366020 2.7487018 13.364339 110-119
TCACA 4912165 2.7134848 12.347218 140-149
ACCAC 5167250 2.7091625 15.723689 230-239
GCACT 6645040 2.701798 70.71437 25-29
CGGAT 7393175 2.6900804 63.005474 20-24
CGGCC 9402455 2.6706593 11.414842 120-129
GGCTG 9903815 2.6523921 25.582468 30-34
GGTTG 9360380 2.641238 56.544083 15-19
AGTCA 5309710 2.624849 30.0358 70-79
AGGGA 6926640 2.6027677 9.719367 150-159
AGATG 5802175 2.5668676 17.194342 180-189
CTGGC 8355520 2.500516 34.98516 60-69
TGTTC 6697690 2.4863358 13.05187 140-149
GAGAT 5540430 2.451072 16.798456 180-189
GTTGG 8622355 2.432988 44.225613 15-19
GATGC 6596705 2.4002771 72.51949 25-29
ACTCT 5004795 2.395749 13.673316 200-209
GTCTC 6717345 2.3667564 12.497287 180-189
TCATT 4643395 2.3419065 16.463015 140-149
TTGTC 6306170 2.3409946 15.278899 170-179
CAGAG 5539430 2.3259416 9.596073 140-149
TTCAT 4607720 2.3239138 17.398619 140-149
TCCAT 4854540 2.3238235 11.965981 70-79
CATTT 4604765 2.3224232 17.216877 150-159
ACAGC 4946935 2.321084 12.570042 120-129
CTGGA 6374990 2.3196042 15.238017 180-189
GTTCT 6192180 2.2986789 18.277235 160-169
TCCTC 5778960 2.2752361 14.812545 130-139
TGTAG 5874795 2.2521946 8.564575 210-219
TTGGT 6669015 2.2155192 41.356728 25-29
CACTA 3979765 2.198426 14.3565445 230-239
TTCAC 4579545 2.1921859 17.593122 200-209
GTGAA 4944020 2.1872218 17.04669 190-199
GGCGG 9572335 2.1774757 35.44924 20-24
TAGTC 5064280 2.169461 24.522747 70-79
ATATA 3059860 2.165275 10.900104 110-119
ATTTG 4729095 2.1344748 15.2299385 150-159
GAATC 4308080 2.1296945 15.411886 190-199
TGAAT 4087620 2.1290355 14.662665 190-199
AATAT 2999955 2.122884 12.637698 110-119
GAGTC 5822205 2.1184676 12.60676 210-219
CTCTG 6010070 2.1175582 15.110082 180-189
GCGGA 6843830 2.115114 75.81846 20-24
GTCAA 4247270 2.099633 41.550957 70-79
ACTAC 3799060 2.0986042 12.381031 230-239
CAGCC 5427075 2.094316 11.501519 120-129
TTGGA 5434540 2.0834162 17.210926 170-179
GATAC 4185785 2.069238 13.6367445 150-159
TCTGC 5861690 2.065279 10.953524 210-219
GTTCA 4820455 2.06501 15.751816 140-149
CTCGC 6175070 2.0649946 17.010748 100-109
TCGGC 6877335 2.0581467 8.726053 190-199
AATAG 3423435 2.057661 8.641887 240-247
CCTTG 5832945 2.0551507 43.25761 20-24
TCTCG 5779735 2.0364032 16.856213 100-109
CCTTC 5143120 2.024899 13.787481 200-209
ATAGA 3362145 2.0208228 10.187219 240-247
ATATG 3867110 2.0141828 9.350046 180-189
GCTGA 5513785 2.0062459 40.294876 30-34
AATCG 4052865 2.003529 13.9685335 190-199
CTACT 4183530 2.0026174 8.265908 230-239
CACGG 5789920 1.9995306 7.8029537 200-209
TACAG 4015135 1.9848773 11.752613 160-169
ACACA 3063230 1.9526906 13.711022 110-119
TCCCA 4288300 1.9483248 11.618391 240-247
TTGGG 6861260 1.9360561 70.15341 15-19
TGGAA 4353305 1.9258909 9.624777 25-29
GATAT 3685520 1.9196018 9.159623 180-189
TTGCA 4455665 1.9087396 13.483456 150-159
GCTGT 6023445 1.8992401 9.986844 140-149
GTCCT 5372585 1.89295 12.653092 130-139
TACCA 3425580 1.8922937 12.044093 230-239
GTTGT 5690735 1.8905239 7.4915104 210-219
GGTCA 5190775 1.8887155 9.796189 130-139
ATTGT 4174970 1.8843709 8.660585 170-179
TACTA 3217580 1.8726761 10.878806 230-239
AGTAT 3582110 1.8657408 11.75814 220-229
TTTGC 5022440 1.8644447 11.661031 150-159
CAGGC 5368230 1.8539013 14.233866 140-149
CATAG 3734050 1.8459232 8.373504 210-219
AAAAA 1957965 1.8450801 6.557146 230-239
AGTCT 4270165 1.8292742 13.063751 210-219
GTCAC 4475655 1.8197505 9.400499 140-149
CTGTT 4871485 1.808407 12.579695 140-149
ATGTA 3467505 1.8060488 10.373696 70-79
GAACT 3649990 1.8043684 11.277592 160-169
ATAGT 3377580 1.7592114 7.1696444 220-229
GTGGA 5325120 1.7339749 39.959583 25-29
TTGTA 3832165 1.729646 9.660676 210-219
GTCTG 5464515 1.723005 8.949414 210-219
TCAGC 4236790 1.7226309 11.197607 140-149
TCTGG 5456390 1.7204432 12.322689 180-189
TGGGG 7126470 1.7080033 42.081703 15-19
ATCTC 3563945 1.7060276 7.4435406 100-109
CACCA 3243670 1.7006395 11.381692 230-239
GCAGA 4022845 1.6891454 13.89913 150-159
ATAAT 2381130 1.6849797 5.990573 100-109
AGAAC 2946790 1.6810558 11.406926 160-169
ACGGC 4865785 1.6803834 12.781301 120-129
CTCCA 3663725 1.6645585 8.611307 240-247
CCCAC 3847680 1.6591905 10.638959 240-247
TGACT 3871860 1.6586463 12.467149 190-199
GGAGG 5983630 1.6549251 27.545197 30-34
CAGCT 4059685 1.6506219 9.5174885 150-159
TGCAG 4518470 1.6440905 12.73742 150-159
TTCAG 3800250 1.6279696 10.087486 150-159
CAAAG 2837220 1.6185491 30.161114 70-79
TCAGA 3270855 1.6169435 9.447189 130-139
GACGT 4427805 1.6111012 10.30207 170-179
CACTG 3933490 1.5993124 6.866815 230-239
GAGAA 3131710 1.5987977 11.021539 160-169
CCAGA 3404335 1.5973015 22.968164 60-69
GGACG 5165565 1.5964392 8.351957 170-179
ATAAA 1953250 1.5950297 6.4322686 110-119
GGGGC 6970640 1.5856525 38.925453 15-19
AACCA 2482715 1.5826348 11.934152 70-79
ACGGA 3734905 1.5682428 10.375723 200-209
AACTG 3169230 1.5667051 10.801105 160-169
CAAAA 2018465 1.5644196 20.361614 70-79
GTCCC 4662990 1.5593423 16.440731 50-59
ATGGG 4755295 1.5484276 54.681236 15-19
TGGTT 4658390 1.5475676 16.313557 10-14
CGACT 3805175 1.5471411 9.487963 190-199
TCGAC 3776295 1.5353987 10.109271 190-199
AGCTC 3762420 1.5297571 9.721602 140-149
GGGCG 6692400 1.5223596 45.61252 20-24
ACTGA 3058035 1.5117362 7.4849186 180-189
CTCTT 3642400 1.5109264 9.461273 200-209
CCTCG 4472345 1.495589 9.086072 130-139
TCCAC 3264710 1.483272 10.311615 240-247
AAATA 1809345 1.4775163 5.184278 110-119
CTGGT 4630730 1.4601063 6.9510636 160-169
CCATC 3204400 1.4558709 11.579254 240-247
GCTCT 4131040 1.4555103 9.502874 130-139
AGCCG 4204635 1.4520574 10.888152 120-129
TGGAC 3960345 1.4410112 11.5914135 170-179
GGCCG 5660125 1.4387401 12.108091 120-129
ACACG 3062305 1.4368224 9.34709 110-119
AATTG 2757190 1.4360814 9.570513 170-179
CTCGA 3521235 1.4316943 9.136051 190-199
ATCGG 3926145 1.4285673 9.536019 190-199
CCACC 3303835 1.4246745 8.25233 230-239
CAATA 2118835 1.4230815 7.7709093 100-109
GAGGG 5079420 1.4048429 9.257166 200-209
TAGTT 3089640 1.3945075 8.795076 220-229
CGTAG 3764865 1.3698838 8.37892 210-219
GAATT 2606400 1.3575425 10.620576 170-179
ACTAT 2331145 1.3567586 7.2544775 230-239
CTTGA 3143250 1.3465208 9.512798 200-209
CATGT 3125625 1.3389705 9.730644 70-79
GGGAG 4835810 1.3374662 6.8001757 150-159
ACCAT 2418495 1.335979 10.418004 45-49
AAGTA 2214120 1.3308003 17.849173 70-79
GAGGC 4305070 1.3304996 24.272932 30-34
TATGG 3457655 1.3255463 6.604654 190-199
ACTCG 3234785 1.3152269 9.580875 190-199
GGCTC 4388840 1.3134269 8.5723915 130-139
AAAGT 2174935 1.3072482 22.160143 70-79
CAGAC 2780390 1.3045489 11.719008 60-69
ATCCA 2360460 1.3039204 17.1361 240-247
TTCCA 2722160 1.3030728 7.5183144 230-239
GTGCT 4126585 1.3011451 7.4126506 220-229
ACTTC 2710155 1.2973261 5.79885 210-219
GGTGG 5351985 1.2827119 39.602802 25-29
ACTGT 2992025 1.2817383 5.4748015 230-239
CCATG 3148790 1.2802621 9.743916 70-79
ACGTG 3515755 1.2792426 9.781712 170-179
GGGCC 5005335 1.2722998 31.06367 15-19
GTGTT 3801935 1.263044 7.442247 160-169
TGTGC 3994330 1.2594441 6.3398314 220-229
TATAC 2153160 1.2531688 6.685831 110-119
GGAGT 3837210 1.249479 6.9746695 210-219
CCTCA 2734750 1.2424926 6.127777 130-139
TGATA 2382035 1.2406821 6.9748363 180-189
TGGGC 4632055 1.2405345 33.123817 15-19
TCACG 3043875 1.2376051 8.997701 200-209
TATCA 2121230 1.2345853 5.737773 70-79
GCTCA 3027215 1.2308311 9.2720375 140-149
AGAGA 2384300 1.2172307 12.6433 35-39
GTCCA 2992845 1.2168567 11.14235 70-79
AGTTC 2834550 1.2142783 5.7657676 160-169
GAACC 2552685 1.1977106 8.775659 70-79
GGAAT 2703945 1.1962184 8.42093 170-179
GCGTA 3276565 1.192211 9.402228 210-219
TTGAG 3108405 1.1916556 8.606331 200-209
CTGAT 2777305 1.1897554 5.9516487 180-189
ACAAT 1756810 1.1799333 8.940875 100-109
AGATC 2353805 1.1636008 5.7668715 130-139
GCATA 2346885 1.1601799 7.7726045 210-219
TGCCC 3459295 1.1568167 9.336247 60-69
CTGCG 3855605 1.153848 6.540385 210-219
ATCAT 1975950 1.1500303 5.178576 90-99
ACTGG 3153395 1.1473943 7.1770544 160-169
TATGT 2470120 1.1148876 7.588552 220-229
CTGAA 2245140 1.1098824 12.961148 35-39
GTCTA 2548520 1.0917474 7.7019086 180-189
TAGAT 2084610 1.0857683 8.060661 240-247
CGGCT 3606220 1.0792159 7.565212 130-139
CCCAA 2056055 1.077979 9.3341675 240-247
GCGGT 4012985 1.0747383 7.0677795 130-139
CTCGG 3572595 1.0691531 7.3850217 130-139
ATAAC 1590225 1.0680492 7.278528 80-89
CCAAT 1911815 1.0560883 10.748265 240-247
AAAAG 1520905 1.0549047 13.130373 70-79
AGGTT 2745930 1.0526953 17.210182 10-14
GAAGA 2038105 1.0404915 17.774069 35-39
TGGTC 3297315 1.0396696 7.996686 50-59
TATAA 1468355 1.0390648 7.2923436 240-247
TCTAC 2164800 1.0362698 7.31879 180-189
GTGGT 3622080 1.0220498 7.0235295 50-59
CATCA 1823795 1.0074661 5.956351 230-239
TCTGT 2709370 1.0057802 5.4865303 210-219
CGGAG 3228355 0.9977364 6.5951757 210-219
GCCAC 2584950 0.9975359 5.02993 80-89
AGAGC 2353585 0.98824275 6.3299956 140-149
GTCAG 2708170 0.9853948 5.2566495 130-139
TGTAA 1886995 0.9828407 6.612964 230-239
GAGTT 2557305 0.98038286 5.192941 160-169
CGGTC 3259825 0.9755519 7.1298723 130-139
CGATG 2676720 0.9739514 22.66642 15-19
TACTG 2252195 0.9648063 5.1020484 180-189
TAGAC 1940855 0.9594595 5.302046 110-119
ACTAA 1427820 0.9589724 10.99793 240-247
AGCTT 2200415 0.94262445 6.0192423 150-159
ACCCA 1792480 0.93978804 11.545335 240-247
AAAAT 1147895 0.9373744 5.591001 5
AAGAG 1827280 0.93286127 12.480162 35-39
ATATC 1597790 0.9299358 5.5609846 70-79
GCTTC 2614770 0.92127526 5.584448 150-159
ACCGT 2238115 0.9099922 12.560673 45-49
CTTCC 2276365 0.8962284 5.2704887 230-239
TCCAG 2203905 0.8960828 5.445466 240-247
TGAAG 2017690 0.8926209 9.143883 35-39
CTACC 1954320 0.88791597 6.599268 230-239
ATCAA 1312275 0.8813685 12.948676 70-79
TGCAT 2044940 0.8760214 6.4035883 210-219
CCAGC 2251345 0.8687972 5.660494 80-89
TGCGT 2696085 0.85009706 6.9401484 210-219
AGCTG 2319760 0.84406793 7.6036086 30-34
GAGCT 2319500 0.8439733 5.33897 140-149
CACAA 1320035 0.84147125 5.292176 100-109
AGCCA 1781365 0.83581 7.745532 80-89
AATCC 1475270 0.8149406 10.003819 240-247
CCTTT 1945520 0.807033 5.8493576 50-59
TGAAC 1628760 0.80517566 5.354945 190-199
TACAA 1169285 0.7853316 5.1508865 100-109
ACGAT 1584240 0.7831672 9.071194 10-14
TGAGA 1763135 0.7800064 6.3050385 240-247
TAACC 1401915 0.7744192 6.276082 80-89
GGAAG 2051615 0.7709189 10.078825 30-34
CCCAT 1693325 0.7693368 5.2217007 240-247
AGGAT 1725130 0.7631931 9.215746 10-14
AGACC 1612825 0.7567317 5.444219 240-247
CTAAT 1294295 0.7532976 8.822425 240-247
AAATG 1249080 0.7507615 5.045102 9
AAAGG 1452790 0.741677 7.2817993 10-14
CGGCG 2853045 0.7252119 5.1117806 130-139
CAATC 1309020 0.7231039 9.995291 240-247
TCGAA 1455690 0.7196187 12.320584 70-79
ACGTC 1766055 0.7180579 7.406713 70-79
ATACC 1297850 0.7169336 6.053303 240-247
GCGGC 2810720 0.71445334 5.269979 130-139
GAAGT 1609110 0.71186614 5.0687695 70-79
TAGGT 1848685 0.7087223 5.6199026 10-14
TGGAT 1829680 0.70143646 7.7479334 10-14
AATGG 1566175 0.69287187 5.573912 10-14
GTCGA 1875180 0.682303 8.408212 70-79
GGTCC 2277215 0.681491 9.025501 50-59
GACCG 1895680 0.6546671 12.934284 45-49
CATCC 1438175 0.6534132 7.995091 240-247
AAAGC 1141855 0.6513942 9.0912895 70-79
CGAAC 1385355 0.65000355 8.668073 70-79
AAGGT 1421910 0.6290493 6.4530425 10-14
CTTTG 1681680 0.6242781 5.0740805 50-59
GAAGC 1420550 0.59647226 9.418046 30-34
TAAAA 728430 0.5948381 5.0908866 2
ATAGG 1339460 0.5925737 5.266921 10-14
AAGCT 1165165 0.57599795 15.677257 30-34
CGTCC 1698790 0.56808937 5.8412666 70-79
TTGCC 1605295 0.56560177 6.316752 60-69
GACGA 1287125 0.54044867 6.7050805 40-44
TCAAG 1082045 0.5349077 5.8520684 70-79
GGGAT 1513595 0.49285948 6.276312 10-14
TAAAG 793130 0.47671208 5.062172 9
CGGTT 1491815 0.47038114 9.436297 10-14
AAGCA 817060 0.46610832 5.777457 70-79
TCGAT 1005050 0.43054822 6.692858 10-14
GCGAT 900035 0.32748672 5.2928977 10-14