FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_17_SL254627.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_17_SL254627.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3284106
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3686400 7.938581 56.051945 110-119
CACAG 4807225 6.8633165 38.92739 110-119
TCCCT 5198125 6.343357 89.981316 30-34
CAGTA 4120765 6.2381973 40.529118 60-69
ATACA 2973550 6.039124 29.43561 110-119
GGAGA 5184180 6.005238 90.16979 35-39
GGTGA 5652120 5.7448754 91.124306 45-49
AGTAA 3056665 5.5918016 41.104794 110-119
CCCTG 5269160 5.4623237 53.810158 30-34
GTAAT 3289235 5.279805 36.085365 110-119
GGCCC 5582775 4.916428 56.918373 60-69
AGACG 3765605 4.842612 96.564804 40-44
ACAGT 3188075 4.82625 32.31197 110-119
GTGAC 4210335 4.750952 80.91124 45-49
GAGAC 3691565 4.7473955 126.184105 40-44
GTAGT 3951070 4.7273793 24.336884 70-79
CCCCA 3582545 4.6989765 97.39151 60-69
GTGTC 4383005 4.339653 25.012045 120-129
ACCAG 3014020 4.303142 92.96346 45-49
ACTCC 3077715 4.2803745 91.09285 30-34
AATAC 2073275 4.210712 29.713512 110-119
CCCTT 3432495 4.18873 64.56494 20-24
CGTGT 4226995 4.1851864 24.161377 120-129
GCACA 2927850 4.180116 32.940853 110-119
TGACC 3273950 4.101387 147.69382 45-49
TGGTG 4470250 3.9867606 28.91268 25-29
GACCA 2763935 3.9460933 104.37323 45-49
TAGTA 2454385 3.9397233 25.215303 80-89
CTCAG 3106890 3.892105 29.308546 140-149
CCCAG 3283500 3.879305 80.6551 60-69
CCACT 2786405 3.875231 26.62966 240-247
CACTC 2782355 3.8695986 77.14797 25-29
TGAGG 3694760 3.7553937 68.58998 35-39
AGTAG 2747560 3.7465692 26.599283 70-79
CTTGG 3693790 3.6572552 38.426365 20-24
CAGGG 3798175 3.6408474 33.605747 50-59
GACGG 3787775 3.6308782 109.78814 40-44
CTCCC 3113765 3.5835755 55.44337 30-34
AGGAG 3085735 3.5744464 89.54333 35-39
CTGAG 3155200 3.5603347 95.55771 35-39
GCCCC 3588100 3.507994 72.42248 60-69
TGTCT 3001525 3.4983354 20.676432 180-189
ACGGT 3097755 3.4955137 91.721855 40-44
ATGGT 2919765 3.4934425 24.640932 190-199
AGGCT 3034015 3.4235892 44.82265 30-34
TCTTG 2932170 3.4175007 19.114454 170-179
AAAAA 1202165 3.3625307 14.572819 230-239
AGATA 1833060 3.3533633 18.076078 150-159
GCCCT 3230415 3.3488398 33.74163 20-24
TGTCC 3004705 3.3027792 18.97661 120-129
CTTCA 2238150 3.3005345 16.894266 200-209
GAGGA 2844690 3.2952254 125.05818 35-39
CCAGG 3052475 3.248436 38.692184 50-59
CCTGG 3467550 3.2379043 31.515606 50-59
TCAGG 2830795 3.1942754 19.339968 140-149
GATGG 3138670 3.190178 33.263546 15-19
ATGCA 2102190 3.182389 76.34956 25-29
TTCCC 2606010 3.180157 54.547333 50-59
GGGTT 3559475 3.1744924 42.9502 50-59
CTCTC 2576695 3.1443832 13.244763 100-109
CCGTG 3308090 3.089005 24.065216 120-129
GGATG 2986160 3.035165 56.038624 20-24
CCAGT 2420070 3.0317023 40.34376 60-69
TGGAG 2952630 3.0010848 32.37225 25-29
TGCAC 2386965 2.990231 99.91709 25-29
ACAGA 1724730 2.9756603 13.9290695 140-149
GGTTC 2982180 2.9526837 48.231384 50-59
TCTCA 1996930 2.9448144 20.190817 130-139
TCGCA 2350125 2.9440796 23.882544 100-109
TCTCT 2246975 2.9074512 15.576066 180-189
CGCAC 2440980 2.8839061 23.954653 100-109
TGGCC 3084195 2.8799381 38.711246 60-69
GACTC 2291625 2.8707955 20.1552 190-199
TACAC 1706390 2.8678362 21.253878 110-119
AGGGT 2741260 2.786246 50.3354 50-59
TTCTT 2022505 2.7748885 21.388569 170-179
GCCGT 2965605 2.7692018 22.748674 120-129
GTTCC 2515140 2.7646482 54.604855 50-59
CAGAT 1814535 2.7469237 15.06803 150-159
CGGAT 2414605 2.7246456 63.046318 20-24
GCACT 2170695 2.719302 70.455154 25-29
CGGTG 3232855 2.719148 66.98551 40-44
GGTTG 3023635 2.6966074 58.562492 15-19
ACCAC 1699865 2.694317 15.1890545 230-239
CCTGA 2145755 2.6880586 62.699917 30-34
AATAA 1077540 2.6445677 12.873369 110-119
GGCTG 3129575 2.6322792 25.12906 30-34
TCACA 1559210 2.6204786 11.525153 140-149
AGTCA 1729235 2.6177928 28.483124 70-79
CGGCC 2956030 2.6032052 10.679236 120-129
AGGGA 2240860 2.595762 9.572526 150-159
GAGAT 1898910 2.5893514 17.128729 180-189
TCAAA 1262610 2.5642943 52.568684 70-79
AGATG 1837095 2.5050607 16.578138 180-189
GTTGG 2791640 2.4897041 45.384846 15-19
CTGGC 2644060 2.468952 34.54232 60-69
TGTTC 2106620 2.4553063 12.818166 140-149
GATGC 2148350 2.4242032 73.816864 25-29
GTCTC 2177430 2.3934364 12.607195 180-189
TTGTC 2005115 2.3370004 15.090094 170-179
ACTCT 1576100 2.3242288 12.659772 200-209
CATTT 1482055 2.3174007 17.534527 150-159
CTGGA 2051570 2.3149962 14.994978 180-189
TCATT 1479900 2.314031 16.203499 150-159
TCCAT 1555425 2.29374 12.045712 70-79
GTTCT 1958340 2.282483 17.79654 160-169
TTCAT 1458735 2.2809367 17.169588 140-149
CAGAG 1770205 2.2765038 9.292114 140-149
TCCTC 1857455 2.2666829 14.740589 130-139
TTGGT 2144050 2.2509181 41.688896 25-29
ACAGC 1571700 2.2439296 11.804548 120-129
GGCGG 3107275 2.2201886 36.101192 20-24
TGTAG 1846310 2.2090743 8.33842 210-219
GTGAA 1612430 2.1987076 16.821964 190-199
TTCAC 1487670 2.1938236 17.371872 200-209
CACTA 1299420 2.1838639 14.088633 240-247
GCGGA 2267940 2.1739976 76.130714 20-24
ATATA 999115 2.151572 10.573335 110-119
TGAAT 1334260 2.1417239 14.595216 190-199
TAGTC 1605070 2.1320353 24.500452 70-79
ATTTG 1513725 2.1320095 15.212544 150-159
AATAT 988365 2.1284223 12.687629 110-119
GAATC 1401715 2.1219788 15.177519 190-199
CTCTG 1928830 2.120175 14.874146 180-189
TCTGC 1927265 2.1184545 11.0203285 210-219
GAGTC 1865225 2.1047242 12.636215 210-219
CCTTG 1880405 2.0669458 43.195324 20-24
CAGCC 1746610 2.0635397 10.876203 120-129
TTGGA 1723890 2.062601 16.585222 170-179
ACTAC 1227010 2.0621684 11.59168 230-239
CCTTC 1667870 2.035329 13.543931 200-209
GTTCA 1531775 2.0346766 15.485313 140-149
TCTCG 1843295 2.0261545 16.334318 100-109
GATAC 1335970 2.0224507 13.020642 150-159
TCGGC 2159410 2.016399 8.796304 190-199
GATAT 1247330 2.0021858 9.2979 180-189
CTCGC 1927490 1.99815 16.652725 100-109
CTACT 1354120 1.9968812 7.880518 230-239
ATATG 1235035 1.9824502 9.171408 180-189
TTGGG 2214480 1.9749682 71.76943 15-19
AATAG 1077195 1.9705992 7.944158 110-119
AATCG 1296920 1.9633356 13.901274 190-199
GTCAA 1296885 1.9632822 38.48668 70-79
ATAGA 1062760 1.9441919 9.537783 240-247
ACACA 1013250 1.9407675 12.895284 110-119
CACGG 1818340 1.935073 7.5326343 200-209
GTTGT 1823955 1.9148684 7.591277 210-219
TACAG 1263335 1.9124931 11.402199 160-169
GCTGA 1694570 1.9121567 38.70814 30-34
TTGCA 1435235 1.9064411 13.310758 150-159
GAACT 1258815 1.9056504 10.99716 160-169
GTCAC 1508010 1.8891346 9.13135 140-149
TGGAA 1376325 1.8767551 9.247801 170-179
GCTGT 1891595 1.8728855 9.840001 140-149
TTTGC 1606115 1.8719583 11.594216 150-159
AGTCT 1408145 1.8704572 13.069744 210-219
GTCCT 1698445 1.866935 12.844939 130-139
TCCCA 1341770 1.866085 11.194002 240-247
TACCA 1099325 1.8475753 11.843447 230-239
GGTCA 1632010 1.8415638 9.352942 130-139
ATTGT 1301915 1.8336852 8.681463 170-179
TACTA 1026955 1.8300762 9.989429 230-239
GTCTG 1840655 1.8224493 8.9115095 210-219
AGTAT 1132790 1.8183287 11.475736 220-229
CAGGC 1694990 1.8038039 13.444176 140-149
CATAG 1189470 1.8006728 8.050298 210-219
TCAGC 1436970 1.8001405 10.856147 140-149
CTGTT 1532955 1.7866887 12.43221 140-149
CTCCA 1271605 1.7685021 8.466135 240-247
CACCA 1109800 1.7590533 10.889845 230-239
TGGGG 2321725 1.7589873 43.04669 15-19
ATGTA 1081495 1.7359914 10.184323 70-79
TTCAG 1295790 1.7212147 9.8707285 150-159
GTGGA 1689965 1.7176986 39.8274 25-29
ATAGT 1069635 1.7169539 7.073384 220-229
TTGTA 1211410 1.7062131 9.622915 210-219
TCTGG 1719495 1.7024877 12.248345 180-189
ATCTC 1152290 1.6992484 6.7108073 100-109
CCAGA 1188065 1.6962104 22.377758 60-69
CCCAC 1259690 1.6522484 10.367964 240-247
GCAGA 1282470 1.6492711 13.293485 150-159
AGAAC 953600 1.6452368 10.317836 160-169
GGAGG 1898005 1.6388159 27.04758 30-34
CAGCT 1302375 1.6315285 9.005057 150-159
ACGGC 1525540 1.6234757 11.877385 120-129
TGCAG 1437235 1.6217792 12.5197315 150-159
GGGGC 2268650 1.62098 39.581764 15-19
ATAAT 752055 1.6195338 5.7463603 110-119
TGACT 1218095 1.6180112 11.892229 190-199
GACGT 1406390 1.5869735 9.946332 170-179
CACTG 1258130 1.5761017 6.4797635 230-239
AACCA 819695 1.5700346 11.705521 70-79
GGACG 1629360 1.5618688 7.8353863 170-179
GGGCG 2177500 1.5558522 47.030956 20-24
ATGGG 1526855 1.5519118 54.443783 15-19
TGGTT 1477740 1.5513966 16.426104 10-14
GTCCC 1493580 1.5483333 16.36554 50-59
CAAAA 668755 1.5479131 19.399183 70-79
ATAAA 630680 1.5478554 6.3777323 110-119
GAGAA 991855 1.5414011 10.37189 160-169
AACTG 1016410 1.538687 10.173809 160-169
CGTAG 1359250 1.5337807 8.377032 210-219
CGACT 1217235 1.5248708 8.989295 190-199
ACTGA 1004645 1.5208763 7.4541674 180-189
ACGGA 1182360 1.5205286 9.792249 200-209
CTCTT 1175025 1.520412 8.844903 200-209
ATCGG 1339565 1.5115682 9.666877 190-199
CAAAG 871740 1.5040046 26.724566 70-79
TCGAC 1192295 1.4936279 9.783125 190-199
ACACG 1043055 1.4891785 8.95979 120-129
AAATA 602890 1.4796513 5.536449 6
AGCTC 1181040 1.4795284 9.309755 140-149
TCAGA 975680 1.4770278 8.665381 130-139
CCTCG 1423185 1.4753579 8.883674 130-139
CCACC 1111655 1.458081 7.9492435 230-239
CTGGT 1461465 1.4470098 6.9221272 160-169
GCTCT 1310680 1.4407028 9.120826 130-139
TCCAC 1035505 1.4401429 9.988726 240-247
AGAGA 921250 1.431677 12.947607 35-39
AGCCG 1336995 1.4228265 10.591472 120-129
CCATC 1020030 1.4186207 10.843841 240-247
GGCCG 1782475 1.4139314 11.622282 120-129
AATTG 873740 1.4025077 9.616813 170-179
CTTGA 1055395 1.4018948 9.283314 200-209
TGGAC 1241210 1.4005842 10.672628 170-179
CTCGA 1115855 1.3978686 8.847706 190-199
GAGGG 1616685 1.3959126 8.948709 200-209
CAATA 684365 1.3899091 7.600977 100-109
ACTAT 768805 1.3700422 7.452347 230-239
TAGTT 970320 1.3666494 8.205395 220-229
AGATC 898690 1.3604771 5.6279645 130-139
GAGGC 1412410 1.3539053 23.704594 30-34
CAGAC 941415 1.3440661 11.242294 60-69
GAATT 831885 1.335323 10.524654 170-179
TATGG 1110370 1.3285363 6.6602488 180-189
GGGAG 1537025 1.3271309 6.6948094 150-159
ATCCA 784260 1.3180628 15.8393345 240-247
CATGT 989410 1.314246 9.666018 70-79
ACCAT 780550 1.3118275 10.381528 45-49
TTCCA 887025 1.3080701 7.303562 230-239
TCACG 1040485 1.3034505 8.849805 200-209
GGCTC 1389255 1.2972488 8.291584 130-139
GGTGG 1700560 1.2883798 39.99411 25-29
GTGCT 1298940 1.2860923 7.3366337 220-229
GGAAT 940380 1.2823011 8.690197 170-179
TATAC 717920 1.279363 6.3223867 110-119
ACTTC 865250 1.275959 5.551388 220-229
ACTGT 955485 1.269183 5.299475 230-239
GTGTT 1207195 1.2673665 7.6231065 160-169
GGGCC 1594380 1.2647268 30.912586 15-19
ACTCG 1009410 1.2645217 9.222029 190-199
CCATG 1003030 1.2565291 9.684178 70-79
ACGTG 1109080 1.2514883 9.340901 170-179
AAGTA 680035 1.2440424 16.350603 70-79
TATCA 695895 1.2401135 5.5165896 70-79
TGGGC 1472255 1.2383107 33.1174 15-19
CTGAA 814815 1.2335032 13.299676 35-39
TGTGC 1245755 1.2334334 6.319464 220-229
CCTCA 886790 1.2333153 5.723331 130-139
GTCCA 981420 1.2294575 11.21522 70-79
GGAGT 1197185 1.2168317 6.956061 210-219
AAAGT 664235 1.2151382 19.834185 70-79
GCTCA 963550 1.2070712 9.181172 140-149
TGATA 750505 1.2046936 6.7658405 180-189
TTGAG 1002855 1.1998966 8.3708315 200-209
GCGTA 1049515 1.1842752 8.94668 210-219
GAAGA 757775 1.1776271 17.842201 35-39
AGTTC 882565 1.1723224 5.5687137 160-169
GAACC 819720 1.1703212 8.609339 70-79
CTGAT 873445 1.1602082 6.210995 180-189
GCATA 764875 1.1579018 7.911551 210-219
ACAAT 560590 1.1385287 8.430649 100-109
CTGCG 1217560 1.1369246 6.3865867 210-219
TGCCC 1087395 1.127258 9.241569 60-69
ACTGG 991020 1.1182692 6.867273 160-169
TAGAT 691860 1.110558 8.02964 240-247
TATGT 786010 1.1070576 7.454785 220-229
TCCAG 881440 1.1042094 5.69128 240-247
AAAAG 524435 1.0933934 12.654945 70-79
CCCAA 685405 1.0863794 8.953753 240-247
GTCTA 816770 1.0849261 7.262185 180-189
AAGAG 695910 1.0814853 12.665537 35-39
AGAGC 839480 1.079581 6.3811717 140-149
AGGTT 890255 1.0651729 18.029322 10-14
GCGGT 1254210 1.0549135 6.954746 130-139
ATATC 591350 1.05381 5.663135 70-79
CGGCT 1121265 1.047007 7.280825 130-139
CTCGG 1117530 1.0435193 7.1338935 130-139
ATAAC 510630 1.0370625 6.957058 80-89
TGGTC 1044740 1.0344065 8.044922 50-59
CCAAT 609705 1.0246978 10.081322 240-247
GTGGT 1142865 1.019256 7.1282125 50-59
TCTGT 873155 1.017679 5.1733813 210-219
ACCCA 641030 1.0160443 10.930999 240-247
TCTAC 686875 1.0129145 6.950898 180-189
TATAA 468030 1.0078923 7.2340293 240-247
CATCA 598295 1.0055215 5.926186 230-239
CGGAG 1016525 0.97441864 6.4771495 210-219
TACTG 732890 0.97350734 5.041184 180-189
CGGTC 1030545 0.9622951 6.9753146 130-139
GTCAG 848855 0.95784986 5.056601 130-139
CGATG 841590 0.949652 22.201763 15-19
TGTAA 591440 0.94936603 6.2940397 230-239
GAGTT 785395 0.93970996 5.10929 160-169
ACTAA 462025 0.93834835 10.909414 240-247
GCTTC 851725 0.93621826 5.132398 150-159
TAGAC 615995 0.93252075 5.0863233 110-119
CTTCC 760715 0.928313 5.084121 230-239
TGAAG 673120 0.9178656 9.466652 35-39
CCAGC 775620 0.9163595 5.892525 80-89
ACCGT 731040 0.9157983 12.675619 45-49
AGCTT 688795 0.91493523 5.4758625 150-159
TGAAC 598440 0.90594506 5.209143 190-199
TGCAT 668330 0.88775134 6.6787643 210-219
GGAAG 755075 0.874662 10.39272 30-34
CTACC 622135 0.86524284 6.4415994 230-239
AGACC 605175 0.86401355 5.514268 240-247
ATCAA 425100 0.8633558 12.398946 70-79
TGCGT 862605 0.85407305 6.8096914 210-219
GTATG 705210 0.8437701 5.362264 220-229
AGCTG 743490 0.83895576 7.1842637 30-34
AATCC 498660 0.8380706 9.500176 240-247
GAGCT 727175 0.82054585 5.284825 140-149
ACGTC 650385 0.8147591 7.155128 70-79
CCTTT 627605 0.8120833 6.122596 50-59
AGCCA 562190 0.8026434 7.3900943 80-89
TGAGA 571515 0.7793172 5.909516 240-247
TAACC 459515 0.7722817 6.521603 80-89
CTAAT 420950 0.75015026 8.663487 240-247
AAAGG 480555 0.74681085 7.2394037 10-14
AAATG 407210 0.7449419 5.1568127 9
TCGAA 488920 0.74014884 12.66197 70-79
TAGGT 613765 0.734358 6.2355094 10-14
GACCG 686940 0.73103976 13.224327 45-49
AGGAT 527290 0.719012 8.456085 10-14
ATACC 422200 0.70956844 5.817165 240-247
TGGAT 591845 0.70813113 7.2486615 10-14
GGTCC 744855 0.6955255 9.023648 50-59
CAATC 412005 0.6924342 9.489538 240-247
GAAGT 507170 0.6915764 5.116297 70-79
AATGG 493905 0.67348826 5.6315536 10-14
GTCGA 593365 0.6695544 8.560865 70-79
CATCC 465345 0.64718497 7.3220453 240-247
AAGGT 474385 0.64687085 6.965698 10-14
CGAAC 452935 0.64665914 8.785802 70-79
ACGAT 421200 0.6376313 8.330593 10-14
CTTTG 544765 0.6349341 5.387149 50-59
TTTCG 535900 0.6246018 5.112515 100-109
AAAGC 361605 0.62387365 8.673144 70-79
GAAGC 460415 0.592099 9.401815 30-34
ATAGG 427800 0.58334756 5.593166 10-14
CGTCC 550940 0.57113695 5.766113 70-79
TTGCC 513735 0.5646988 6.0568137 60-69
AAGCT 366090 0.5542034 14.522845 30-34
TCAAG 358625 0.5429025 5.565443 70-79
GACGA 392615 0.50490737 6.331181 40-44
GGGAT 467155 0.47482136 6.22551 10-14
CGGTT 477715 0.47298998 9.577435 10-14
AAGCA 273390 0.47167715 5.5230503 70-79
TAAAG 254605 0.46576932 5.109378 9
TCGAT 326515 0.43371406 6.625699 10-14
GCGAT 293385 0.3310563 5.6285334 10-14