FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_16_SL254626.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_16_SL254626.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4386903
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 4924545 8.041637 56.570435 110-119
CACAG 6502985 6.9395165 39.59018 110-119
TCCCT 6996830 6.321155 90.25607 30-34
CAGTA 5442310 6.1931653 39.592957 60-69
ATACA 4020695 6.156964 30.403746 110-119
GGAGA 6966370 6.0917177 91.46506 35-39
GGTGA 7545430 5.776113 92.17509 45-49
AGTAA 4083680 5.66077 41.866592 110-119
CCCTG 7007275 5.3739014 54.312122 30-34
GTAAT 4407505 5.348538 36.425625 110-119
AGACG 5093890 4.920672 97.179726 40-44
GTAGT 5442755 4.908231 24.601074 70-79
ACAGT 4270690 4.859901 32.880104 110-119
GGCCC 7373470 4.8001842 55.12956 60-69
GTGAC 5620270 4.7528105 81.49041 45-49
GAGAC 4915065 4.747928 126.908195 40-44
CCCCA 4827940 4.6722445 96.19341 60-69
GTGTC 5874985 4.349292 24.933212 120-129
ACTCC 4162975 4.2961593 91.52051 30-34
AATAC 2770640 4.2427316 30.634085 110-119
ACCAG 3969325 4.2357774 91.45565 45-49
CGTGT 5686105 4.209463 24.1324 120-129
GCACA 3922560 4.1858735 32.72499 110-119
CCCTT 4597705 4.1537104 62.326263 20-24
TAGTA 3422560 4.153301 28.783104 80-89
TGACC 4377260 4.08919 147.14456 45-49
TGGTG 5960505 3.9944148 28.658163 25-29
GACCA 3640900 3.885306 102.62151 45-49
AGTAG 3767165 3.880631 26.570963 70-79
CCCAG 4428885 3.8798637 80.69647 60-69
CTCAG 4148415 3.875406 29.061144 140-149
CACTC 3743115 3.8628669 78.28514 25-29
TGAGG 5012895 3.837428 69.235 35-39
CCACT 3677010 3.7946472 26.417135 240-247
CTTGG 4939595 3.656816 37.088696 20-24
AGGAG 4151240 3.6300368 90.924324 35-39
CTCCC 4283425 3.628886 55.32295 30-34
GACGG 5037725 3.6163728 109.87818 40-44
CAGGG 4990375 3.5823827 32.845192 50-59
CTGAG 4234600 3.5810115 96.29442 35-39
ATGGT 3935810 3.5492806 25.32186 190-199
TGTCT 4053100 3.5347087 20.817907 180-189
TCTTG 4030555 3.5150473 19.517385 170-179
ACGGT 4155740 3.5143232 92.32563 40-44
GCCCC 4825850 3.4705756 71.20116 60-69
AGATA 2492090 3.4545186 18.155586 150-159
AAAAA 1600640 3.410634 18.400549 240-247
AGGCT 3996850 3.379957 43.38005 30-34
GAGGA 3821870 3.3420203 126.23607 35-39
CTTCA 3008235 3.3105583 16.760601 200-209
TGTCC 3996520 3.2684014 18.92479 120-129
GCCCT 4246980 3.2570226 32.933147 20-24
ATGCA 2856180 3.2502363 77.8604 25-29
TCAGG 3779760 3.1963732 19.856567 140-149
GATGG 4166135 3.1892238 33.08397 15-19
CCAGG 4013760 3.1829648 37.628258 50-59
GGGTT 4718300 3.161955 42.103756 50-59
CTCTC 3482085 3.1458244 13.303969 100-109
CCTGG 4514240 3.1338887 30.7017 50-59
CCGTG 4508205 3.1296992 23.922092 120-129
TTCCC 3449200 3.1161146 52.87422 50-59
GGATG 3989835 3.0542643 57.691322 20-24
ACAGA 2345150 3.0484674 14.467591 140-149
TGCAC 3205540 2.9945815 100.77988 25-29
TGGAG 3874950 2.9663184 31.295448 25-29
CCAGT 3153625 2.9460835 39.12846 60-69
TCGCA 3147165 2.9400482 23.784777 100-109
TCTCA 2661545 2.9290264 20.46826 130-139
GGTTC 3946435 2.9215732 46.986233 50-59
TACAC 2323175 2.9204714 21.85574 110-119
GACTC 3096985 2.8931706 20.218227 190-199
CGCAC 3286965 2.8795004 23.894474 100-109
TCTCT 2985640 2.8763742 15.588033 180-189
CAGAT 2480085 2.8222532 15.554916 150-159
TTCTT 2744105 2.8191721 21.664907 170-179
TGGCC 4026405 2.795222 37.784782 60-69
GCCGT 3990320 2.7701716 22.628786 120-129
CGGAT 3250505 2.7488065 64.642334 20-24
GGTTG 4089535 2.7405903 59.67012 15-19
AGGGT 3571230 2.733817 49.50543 50-59
GCACT 2925385 2.7328641 71.794754 25-29
CCTGA 2924685 2.7322102 64.0817 30-34
CGGTG 4308020 2.7072914 67.38458 40-44
GTTCC 3290880 2.6913207 53.173958 50-59
TCACA 2131085 2.6789942 11.90206 140-149
ACCAC 2254315 2.6574965 15.267214 230-239
AGGGA 3034870 2.653831 9.636716 150-159
AATAA 1411230 2.632435 12.513657 110-119
AGTCA 2269100 2.582159 28.12468 70-79
GGCTG 4104355 2.579302 24.235678 30-34
CGGCC 3932960 2.5603864 10.7847595 120-129
TCAAA 1662870 2.5463827 52.319233 70-79
AGATG 2451420 2.5252562 16.916397 180-189
GTTGG 3766030 2.523794 46.29095 15-19
TCCAT 2246915 2.4727268 13.621487 70-79
TGTTC 2833120 2.4707642 13.011041 140-149
GATGC 2906100 2.4575586 74.89457 25-29
GAGAT 2368565 2.4399056 16.536217 180-189
CTGGC 3440490 2.3884664 33.66775 60-69
GTCTC 2898325 2.3702846 12.591418 180-189
ACTCT 2145020 2.3605914 12.611198 200-209
TTGTC 2663535 2.322869 15.026841 170-179
CAGAG 2398215 2.3166635 9.308875 140-149
GTTCT 2654320 2.3148327 18.296227 160-169
TCATT 1966450 2.3077314 16.385876 150-159
TCCTC 2545495 2.2996798 14.85063 130-139
CATTT 1957750 2.2975216 17.56806 150-159
TTCAT 1953290 2.2922876 17.023748 140-149
CTGGA 2699085 2.2824953 15.078222 180-189
ACAGC 2134830 2.2781367 12.06752 120-129
TGTAG 2515255 2.2682357 8.621206 210-219
GGCGG 4157420 2.2178216 36.629253 20-24
TTGGT 2799315 2.2099197 41.278404 25-29
TTCAC 1996565 2.197217 17.334791 200-209
GTGAA 2130240 2.1944025 16.961329 190-199
GCGGA 3020550 2.168327 77.24543 20-24
CACTA 1705780 2.144342 14.585933 240-247
ATTTG 2012995 2.1384714 15.578631 150-159
TGAAT 1761470 2.137556 14.821252 190-199
ATATA 1304925 2.1309042 10.351974 110-119
CTCTG 2575380 2.1061766 14.893295 180-189
TTGGA 2335035 2.105715 16.686716 170-179
GAATC 1850380 2.10567 15.202522 190-199
TAGTC 2099410 2.0914388 23.941404 70-79
AATAT 1279715 2.089737 12.207511 110-119
TCTGC 2552900 2.087792 10.688792 210-219
GAGTC 2458635 2.0791574 12.644689 210-219
GATAC 1824865 2.0766346 13.175388 150-159
GTTCA 2083245 2.0753348 15.468194 140-149
GATAT 1697585 2.0600312 9.508838 180-189
ACTAC 1636265 2.0569544 12.437799 230-239
TCTCG 2501725 2.0459404 16.115482 100-109
CAGCC 2323050 2.035076 10.927682 120-129
TTGGG 3004155 2.0132256 73.095276 15-19
ATATG 1656205 2.0098164 9.399272 180-189
CCTTC 2219605 2.0052605 13.42683 200-209
CTACT 1822055 2.005169 8.361392 230-239
TCGGC 2885685 2.0033083 8.674198 190-199
CCTTG 2443610 1.9984136 41.422203 20-24
CTCGC 2584680 1.9821993 16.387518 100-109
AATAG 1421080 1.9698917 8.2895565 240-247
AATCG 1727660 1.9660189 13.866863 190-199
ACACA 1364915 1.9600093 13.270072 110-119
ATAGA 1409880 1.9543666 9.797508 240-247
TTTGC 2234235 1.9484764 11.9546 150-159
GTCAA 1707895 1.9435266 38.014503 70-79
TTGCA 1939395 1.932031 13.648275 150-159
TACAG 1695810 1.9297745 11.676797 160-169
GTTGT 2432290 1.9201716 7.5608635 210-219
CACGG 2411910 1.9126766 7.357012 200-209
GTCAC 2039620 1.9053915 9.1715355 140-149
GAACT 1674220 1.9052058 11.718686 160-169
TCCCA 1844230 1.9032319 11.189108 240-247
GCTGA 2215930 1.8739128 37.703762 30-34
GCTGT 2511030 1.8589329 10.002109 140-149
GGTCA 2192980 1.8545051 9.583186 130-139
GTCCT 2264625 1.8520373 12.823231 130-139
TGGAA 1796645 1.8507596 9.324521 25-29
ATTGT 1726865 1.8345059 8.294884 170-179
AGTAT 1509570 1.8318738 11.771193 220-229
TACCA 1457135 1.8317695 11.774574 230-239
GTCTG 2470685 1.829065 8.806559 210-219
AGTCT 1833045 1.8260849 12.921185 210-219
TACTA 1358540 1.8211945 10.593043 230-239
TCAGC 1941250 1.8134949 11.05742 140-149
ATGTA 1489790 1.8078707 11.772924 70-79
CCAGA 1690565 1.8040491 23.950014 60-69
CAGGC 2263540 1.7950172 13.744084 140-149
CTGTT 2055780 1.7928458 12.690796 140-149
TGGGG 3143085 1.7880174 44.256516 15-19
CATAG 1550380 1.7642801 7.9823976 210-219
TTCAG 1752980 1.746324 10.093724 150-159
CACCA 1478960 1.7434703 10.803722 230-239
TTGTA 1606250 1.7063725 9.813125 210-219
CTCCA 1649010 1.7017663 8.133435 240-247
ATCTC 1546300 1.7017009 6.757627 100-109
GTGGA 2220115 1.6995233 38.753513 25-29
TCTGG 2294290 1.6984786 12.172515 180-189
ATAGT 1386275 1.6822546 7.002077 220-229
GCAGA 1739545 1.6803918 13.672288 150-159
GACGT 1977690 1.6724436 10.067824 170-179
CAGCT 1783870 1.6664727 9.154898 150-159
AGAAC 1275615 1.6581757 10.679268 160-169
CCCAC 1710905 1.6557304 10.323328 240-247
TGCAG 1948055 1.6473829 12.759715 150-159
GGGGC 3070585 1.6380376 40.444492 15-19
GGAGG 2508990 1.6304073 26.926313 30-34
ACGGC 2040855 1.618425 12.072374 120-129
TGACT 1623935 1.6177689 11.7848625 190-199
ATAAT 980280 1.6007683 5.5111313 100-109
GTCCC 2075525 1.5917265 17.752687 50-59
GAGAA 1348150 1.586381 10.686966 160-169
CCATC 1536650 1.5858115 10.6947775 240-247
ATAAA 850070 1.5856763 6.3242097 110-119
GGACG 2199965 1.5792634 7.9218497 170-179
TGGTT 1989940 1.5709584 17.11981 10-14
GGGCG 2933955 1.5651507 47.570683 20-24
CACTG 1658025 1.5489094 6.5563726 230-239
CTCTT 1607350 1.5485257 9.0457 200-209
ATGGG 2016575 1.5437111 53.48798 15-19
AACTG 1353945 1.5407437 10.2714205 160-169
CGACT 1647500 1.5390773 9.235518 190-199
CAAAA 873905 1.528661 19.356314 70-79
AACCA 1063670 1.5274234 11.441758 70-79
ATCGG 1798780 1.5211477 9.660142 190-199
TCGAC 1626520 1.5194777 9.699288 190-199
CAAAG 1168805 1.5193329 26.319267 70-79
ACGGA 1560545 1.5074787 9.798848 200-209
ACTGA 1324220 1.5069176 7.477754 180-189
ACACG 1407460 1.5019398 9.111797 120-129
AGCTC 1607015 1.5012565 9.269384 140-149
CCTCG 1934375 1.4834783 9.204983 130-139
GCTCT 1804965 1.476122 9.341496 130-139
CTGGT 1986600 1.4706936 6.977559 160-169
AAATA 784700 1.4637386 5.0302496 110-119
GTCCA 1562225 1.4594141 12.683847 70-79
CCACC 1505605 1.457051 8.057701 230-239
TCAGA 1276235 1.4523122 8.771577 130-139
TGGAC 1712175 1.4479097 10.917075 170-179
GAGGG 2213890 1.4386433 9.326638 200-209
CTTGA 1436300 1.4308463 9.404736 200-209
AGCCG 1803540 1.4302309 10.661824 120-129
CAGAC 1339775 1.4297115 12.878385 60-69
TCCAC 1374505 1.4184791 9.785452 240-247
CTCGA 1511015 1.4115745 8.978418 190-199
GGCCG 2372630 1.3982164 11.595547 120-129
CGTAG 1648425 1.3939992 8.137496 210-219
AATTG 1148585 1.393816 9.135267 170-179
TAGTT 1296650 1.3774744 8.652945 220-229
GGGAG 2096585 1.3624158 6.7872252 150-159
CATGT 1354595 1.3494517 11.194829 70-79
TATGG 1485965 1.3400308 6.70061 180-189
GAGGC 1864380 1.3383608 23.51697 30-34
CAATA 868640 1.3301642 7.456807 100-109
GGCTC 1912205 1.3274965 8.366484 130-139
GAATT 1091705 1.3247917 10.128908 170-179
ACTAT 978505 1.3117375 7.189065 230-239
ACCAT 1027280 1.2913973 10.388884 45-49
GTGCT 1738265 1.2868495 7.3868937 220-229
GGTGG 2255445 1.2830627 39.161934 25-29
ATCCA 1020330 1.2826604 15.175091 240-247
GGAAT 1244855 1.2823498 8.270516 170-179
CCATG 1369945 1.2797883 11.108389 70-79
ACGTG 1512385 1.2789563 9.515694 170-179
GTGTT 1618060 1.2773777 7.432164 160-169
ACTCG 1364675 1.2748649 9.119313 190-199
TATAC 947345 1.269966 6.2690454 110-119
ACTGT 1263560 1.2587624 5.2315507 230-239
TTCCA 1141450 1.2561641 7.350773 230-239
CTGAA 1102015 1.2540557 13.4945545 35-39
ACTTC 1131380 1.245082 5.5099072 220-229
TGATA 1024935 1.2437658 6.85089 180-189
GGGCC 2107705 1.2420932 29.679937 15-19
AAGTA 893170 1.2381064 15.953047 70-79
CCTCA 1196035 1.2342993 5.7759004 130-139
AGAGA 1045245 1.2299497 12.563241 35-39
GGAGT 1606065 1.2294612 6.938624 210-219
GCTCA 1300415 1.2148341 9.03665 140-149
TTGAG 1343805 1.211832 8.621597 200-209
TGGGC 1925230 1.2098732 32.037975 15-19
GCGTA 1425350 1.2053547 9.034652 210-219
AAAGT 868545 1.2039713 19.670187 70-79
TGTGC 1622680 1.2012811 6.2191377 220-229
GAAGA 1015160 1.1945484 18.039936 35-39
AGTTC 1195370 1.1908312 5.9516606 160-169
GAACC 1102605 1.1766206 8.554612 70-79
TATCA 876445 1.1749208 5.5575733 70-79
AGATC 1029215 1.171212 5.5940256 130-139
TGCCC 1524145 1.1688715 10.569068 60-69
CTGAT 1163620 1.1592017 5.998894 180-189
CTGCG 1657225 1.1504834 6.300693 210-219
AGTGA 1110525 1.1439738 5.065463 160-169
AGAGC 1177930 1.1378744 6.5698686 140-149
ACTGG 1336495 1.130214 6.9435606 160-169
TCACG 1200035 1.1210599 8.493309 200-209
GTCTA 1119110 1.1148608 7.543991 180-189
GCATA 970635 1.10455 7.279458 210-219
TAGAT 909970 1.1042551 7.769031 240-247
TATGT 1037525 1.1021972 7.36742 220-229
AAGAG 935740 1.1010942 12.455726 35-39
CCCAA 932245 1.098976 9.314327 240-247
ACAAT 715110 1.095061 8.353545 100-109
TTGGC 1463280 1.0832762 5.1266203 170-179
TGGTC 1461570 1.0820103 9.398142 50-59
TCCAG 1155725 1.0796663 5.395313 240-247
AGGTT 1194075 1.0768069 18.189562 10-14
GTGGT 1599730 1.0720543 8.317235 50-59
GCGGT 1698070 1.067119 6.9305234 130-139
CTCGG 1529705 1.0619563 7.5915365 130-139
AAAAG 670200 1.0612307 12.984076 70-79
CGGCT 1513735 1.0508693 7.5479784 130-139
TCTAC 953590 1.0494244 7.1422434 180-189
ATATC 779005 1.0442975 5.491694 70-79
TATAA 636030 1.0386182 7.424748 240-247
ATAAC 669385 1.0250415 6.6822824 80-89
CCAAT 814675 1.0241308 10.394101 240-247
TCTGT 1148615 1.0017072 5.2856307 210-219
CATCA 780505 0.98117554 5.6631846 230-239
CGGTC 1412685 0.98071826 6.976329 130-139
GAGTT 1082845 0.9765006 5.5666227 160-169
ACCCA 825475 0.97311026 11.002529 240-247
TACTG 975345 0.9716416 5.077767 180-189
ACCGT 1030325 0.9625189 14.629654 45-49
CGATG 1137175 0.961658 22.379368 15-19
CGGAG 1336970 0.95975506 6.416873 210-219
TGTAA 786675 0.9546356 6.3691235 230-239
AGCTT 957085 0.9534509 5.7551517 150-159
CCATT 860750 0.94725424 5.051875 45-49
GCTTC 1154520 0.9441802 5.4166813 150-159
AAAAT 505255 0.9424765 5.4517226 5
ACTAA 615130 0.94195974 11.467395 240-247
TGAAC 803855 0.9147598 5.2379208 190-199
CTTCC 1008115 0.9107625 5.1758075 230-239
TGAAG 882420 0.90899825 9.768967 35-39
CCAGC 1032065 0.90412635 5.871793 80-89
ACGTC 951705 0.8890729 8.3466625 70-79
CTACC 851175 0.8784063 6.5806837 230-239
AGACC 815415 0.8701521 5.110288 240-247
GGAAG 994185 0.86936146 10.038324 30-34
CCTTT 900885 0.8679153 7.013401 50-59
ATCAA 565700 0.86626667 12.5508995 70-79
AGCTG 1019745 0.86235267 7.258827 30-34
GTATG 953555 0.85990787 5.268597 220-229
GAGCT 1003570 0.8486742 5.370381 140-149
AATCC 673350 0.84647065 9.775834 240-247
TGCAT 846710 0.843495 6.0211825 210-219
TGCGT 1130535 0.8369428 6.718398 210-219
AGCCA 750850 0.80125296 7.193731 80-89
GACCG 994495 0.7886476 14.59059 45-49
AAAGG 665390 0.7829708 7.17922 10-14
CGTCC 998305 0.7656033 6.6219077 70-79
CGTTG 1027685 0.7608023 5.1515884 170-179
TGAGA 733420 0.75551045 5.8044825 240-247
CTAAT 557815 0.7477804 9.091671 240-247
GGTCC 1074005 0.74559885 10.27033 50-59
TAGGT 818155 0.73780537 6.261312 10-14
TAACC 579980 0.72909486 5.992646 80-89
AGGAT 694660 0.71558297 8.275687 10-14
TCGAA 628655 0.7153881 12.348651 70-79
CAATC 566490 0.71213657 9.792328 240-247
ATACC 563920 0.7089058 5.7008233 240-247
GCGGC 1196105 0.7048775 5.0284657 130-139
CTTTG 796845 0.6949286 6.1535044 50-59
TGGAT 769490 0.69391966 6.979951 10-14
GCGTT 934740 0.6919944 5.152685 170-179
ACGAT 593295 0.67514974 8.945944 10-14
GTCGA 789610 0.6677378 8.489141 70-79
CGTGG 1058710 0.66532576 5.5019703 50-59
AATGG 638350 0.65757704 5.572327 10-14
AAGGT 637290 0.656485 6.4672565 10-14
CGAAC 614035 0.65525395 8.634858 70-79
AAAGC 496260 0.64508986 8.887799 70-79
TTGCC 760460 0.6219132 7.179063 60-69
CATCC 589370 0.60822546 6.8505325 240-247
GAAGC 612635 0.5918023 8.993838 30-34
ATAGG 562240 0.57917464 5.5481606 10-14
AAGCT 497740 0.5664113 14.800132 30-34
TCAAG 474125 0.5395382 5.7219453 70-79
GACGA 533920 0.51576394 6.2512293 40-44
CGGTT 665525 0.4926927 10.416201 10-14
GGGAT 619990 0.47460946 6.0843024 10-14
AAGCA 365110 0.47460756 5.6299725 70-79
TCGAT 430435 0.42880064 6.6100526 10-14
GCGAT 379245 0.3207105 5.080695 10-14