FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_11_SL254621.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_11_SL254621.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4513515
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 4785225 7.4542603 52.885406 110-119
CACAG 6367315 6.4379797 36.003513 110-119
TCCCT 6923445 6.1785607 86.99317 30-34
CAGTA 5395470 5.88137 38.46493 60-69
AAAAA 2966915 5.764835 35.884052 230-239
GGAGA 6881350 5.740693 84.3996 35-39
ATACA 3956220 5.7164536 28.744476 110-119
GGTGA 7431740 5.551259 88.09981 45-49
CCCTG 7071665 5.317157 51.16406 30-34
AGTAA 4013895 5.268184 38.795746 110-119
GTAAT 4328025 5.0862174 34.87083 110-119
GTAGT 5272845 4.6746936 23.91643 70-79
GGCCC 7360680 4.6630335 52.95051 60-69
GAGAC 5059580 4.6468267 118.21267 40-44
AGACG 4978570 4.5724254 91.01599 40-44
ACAGT 4168735 4.544159 30.835192 110-119
GTGAC 5518195 4.537841 77.739624 45-49
CCCCA 4869165 4.5014596 89.3694 60-69
ACTCC 4508225 4.4932485 91.89693 30-34
GTGTC 5757990 4.2396827 24.515936 120-129
ACCAG 4088340 4.133713 84.940605 45-49
GCACA 4083275 4.128592 29.933214 110-119
CGTGT 5552700 4.088526 23.503088 120-129
TGGTG 5984855 4.002808 28.842241 25-29
CCCTT 4480340 3.998306 61.690002 20-24
CACTC 4009300 3.9959805 76.53847 25-29
AATAC 2709840 3.9155245 28.784092 110-119
TGACC 4250545 3.848116 138.49014 45-49
TAGTA 3229305 3.7950215 24.997763 80-89
TGAGG 5072710 3.789143 67.66874 35-39
CCCAG 4451525 3.7381473 75.14165 60-69
CCACT 3745105 3.7326634 25.117655 240-247
CTCAG 4109270 3.7202165 27.160597 140-149
GACCA 3673355 3.7141225 94.955414 45-49
AGTAG 3662885 3.62678 26.090162 70-79
CTTGG 4856420 3.575845 36.706127 20-24
CTCCC 4260080 3.526362 53.880093 30-34
TGTCT 4030620 3.522424 20.169168 170-179
CTGAG 4267450 3.509302 93.261604 35-39
CAGGG 5045680 3.4959457 31.987925 50-59
AGGAG 4167920 3.477043 84.33305 35-39
GACGG 4951875 3.4309525 103.78853 40-44
TCTTG 3909930 3.4169505 18.692543 170-179
ATGGT 3850575 3.4137661 23.775948 190-199
CTTCA 3163625 3.3993492 15.933595 200-209
ACGGT 4074975 3.3510213 86.528694 40-44
GCCCC 4804400 3.3507426 67.2579 60-69
AGGCT 3961990 3.2581089 41.94315 30-34
AGATA 2478900 3.2535236 16.897402 150-159
GGGTT 4837625 3.2355144 42.159996 50-59
ATGCA 2962750 3.2295668 78.419044 25-29
CCTGG 4684315 3.199283 29.763815 50-59
GAGGA 3833015 3.197652 118.64876 35-39
TTCCC 3538225 3.157552 52.651363 50-59
GCCCT 4198420 3.1567755 32.580833 20-24
TGTCC 3871075 3.1379454 18.409079 120-129
TCAGG 3753465 3.0866306 18.936432 140-149
CCAGT 3409315 3.0865312 38.335514 60-69
CTCTC 3457395 3.0854185 13.060606 100-109
GATGG 4129000 3.0842235 32.53268 15-19
GGATG 4109565 3.0697062 56.97282 20-24
CCAGG 4000550 3.0515182 36.2971 50-59
TGCAC 3314880 3.001037 100.98418 25-29
GGTTC 4046275 2.979325 46.90062 50-59
CCGTG 4353160 2.9731114 23.078382 120-129
TGGAG 3890110 2.90578 30.845213 25-29
ACAGA 2344100 2.853745 13.191465 140-149
GACTC 3114525 2.819651 19.776442 190-199
TACAC 2346945 2.8164592 20.597368 110-119
TCGCA 3110540 2.8160431 23.021732 100-109
GGTTG 4183880 2.7982752 62.008835 15-19
TCTCT 2905240 2.7951365 14.659439 180-189
TTCTT 2684010 2.783948 20.740917 170-179
TCTCA 2587620 2.7804255 19.328499 130-139
GCCGT 4056245 2.770325 21.813702 120-129
GCACT 3057045 2.7676134 70.434715 25-29
CGGAT 3354210 2.7583065 63.650124 20-24
TGGCC 4006600 2.7364182 36.936317 60-69
CAGAT 2509320 2.735302 14.251602 150-159
AGTCA 2505040 2.7306366 27.282167 70-79
CGCAC 3233880 2.7156355 22.7793 100-109
AGGGT 3633775 2.7143068 48.293793 50-59
GTTCC 3308660 2.6820443 52.79344 50-59
ACCAC 2351240 2.6172278 13.892637 230-239
CGGTG 4213575 2.6140027 64.10026 40-44
CCTGA 2867280 2.5958145 59.770096 30-34
GTTGG 3878155 2.5937989 48.61974 15-19
AGGGA 3083585 2.572448 9.309302 150-159
GGCTG 4089740 2.5371783 23.776052 30-34
TCACA 2110045 2.5321665 11.25976 140-149
GATGC 2993895 2.4620044 75.71772 25-29
CGGCC 3854530 2.441867 10.28222 120-129
AATAA 1402205 2.4395149 11.234182 110-119
TCAAA 1660885 2.3998594 48.862083 70-79
ACTCT 2211895 2.376705 12.386558 200-209
CTGGC 3463525 2.3655105 33.24068 60-69
TGTTC 2706115 2.3649175 12.299561 140-149
AGATG 2380440 2.356976 15.626765 180-189
GTCTC 2875945 2.3312795 11.598406 180-189
TCCTC 2591405 2.3125992 13.603631 130-139
GAGAT 2313110 2.29031 15.36456 180-189
TTGGT 2878910 2.2853172 42.497177 25-29
GTTCT 2574625 2.2500064 17.691696 160-169
GGCGG 4285475 2.2399945 37.713943 20-24
CAGAG 2426230 2.2283015 8.91949 140-149
TTGTC 2546210 2.225174 14.516933 170-179
CTGGA 2682610 2.2060218 14.13252 180-189
CATTT 1897930 2.1986034 16.210339 150-159
TCATT 1896285 2.196698 15.246904 140-149
TTCAC 2039505 2.1914701 16.252398 200-209
TCTGC 2702135 2.1903865 10.916093 210-219
GCGGA 3156660 2.1871212 77.14189 20-24
TGTAG 2460120 2.181044 8.375817 210-219
TTCAT 1868025 2.163961 16.421041 140-149
TCCAT 1989280 2.137503 11.739117 70-79
CACGG 2801795 2.1371384 7.230565 200-209
GATAT 1816915 2.135206 9.380461 180-189
TCTCG 2623395 2.1265593 16.09566 100-109
GAACT 1936675 2.1110861 13.015267 160-169
TTGGA 2350990 2.0842938 16.254196 170-179
GTGAA 2097055 2.0763843 15.737308 190-199
TTGGG 3104060 2.0760665 75.52014 15-19
CTCTG 2555115 2.0712104 13.981024 180-189
ACAGC 2046590 2.0693033 10.855441 120-129
TAGTC 2119375 2.068556 23.974926 70-79
TGAAT 1742315 2.0475373 13.900989 190-199
ATTTG 1941395 2.0428162 14.50253 150-159
CACTA 1700240 2.0403783 13.325116 240-247
ATATA 1306815 2.035712 9.759008 110-119
GTCAC 2247010 2.0342698 9.137026 140-149
GTTCA 2077375 2.027563 14.809893 140-149
GTCTG 2708065 1.9939836 8.629798 210-219
CCTTG 2456325 1.99113 40.924755 20-24
GATAC 1824570 1.9888853 12.3056135 150-159
GAGTC 2407780 1.9800178 11.58424 210-219
GAATC 1808235 1.9710792 13.962631 190-199
CCTTC 2195150 1.9589767 12.560558 200-209
TCAGC 2136160 1.9339147 10.889041 140-149
ACTAC 1609370 1.9313297 11.157168 230-239
CTACT 1795835 1.9296441 7.651684 230-239
AATAT 1238500 1.9292932 11.3397875 110-119
CAGCC 2296835 1.9287564 10.105533 120-129
CTCCA 1927955 1.92155 7.982526 240-247
CTCGC 2547495 1.9154514 15.959214 100-109
TTCAG 1952115 1.9053067 10.165512 150-159
TCGGC 2786460 1.9030901 8.186622 190-199
GTTGT 2379440 1.8888311 7.7581477 210-219
ATATG 1604430 1.8854976 9.260421 180-189
ACACA 1405540 1.8837945 11.949264 110-119
ATAGA 1432880 1.8806361 9.16049 240-247
CACCA 1682145 1.8724406 9.922566 230-239
AATAG 1424845 1.8700902 7.7199516 240-247
GTCAA 1709960 1.8639541 36.133636 70-79
ACGGC 2442760 1.8632755 11.775208 120-129
CCAGA 1834400 1.8547584 20.839397 60-69
TGGGG 3278445 1.8474463 44.34598 15-19
TTTGC 2091150 1.8274895 11.265772 150-159
TTGCA 1871945 1.827059 12.749353 150-159
TCCCA 1831245 1.8251613 10.246059 240-247
AATCG 1666575 1.816662 12.659909 190-199
TACAG 1666175 1.8162259 10.562374 160-169
GCTGT 2465980 1.8157333 9.551687 140-149
GGTCA 2197235 1.8068779 9.388251 130-139
GCTGA 2190500 1.8013394 35.470505 30-34
ATCTC 1669420 1.7938098 6.5788994 100-109
GTCCT 2195055 1.779341 11.6760235 130-139
AGTCT 1813035 1.7695615 12.249754 210-219
TGGAA 1778325 1.760796 8.470228 170-179
ATTGT 1659175 1.7458526 8.100722 170-179
TACTA 1338975 1.7323232 9.625418 230-239
AGTAT 1469950 1.727459 10.694005 220-229
TACCA 1434640 1.7216444 10.91708 230-239
CTGTT 1958235 1.7113332 11.99905 140-149
CAGGC 2242930 1.7108502 12.711777 140-149
CATAG 1566220 1.7072692 7.4343944 210-219
TTGTA 1605680 1.6895632 9.678819 210-219
GTGGA 2254310 1.6838931 37.64873 25-29
GGGGC 3199360 1.6722882 40.08693 15-19
GGAGG 2653585 1.6700395 26.894257 30-34
ATGTA 1417710 1.6660674 10.136044 70-79
CCCAC 1792940 1.6575425 9.552688 240-247
ACACG 1637735 1.6559108 8.886812 110-119
TCTGG 2221765 1.6359144 11.586904 180-189
ATAGT 1385560 1.6282854 6.4348364 220-229
CAGCT 1796890 1.6267657 8.802545 150-159
ATCGG 1975570 1.6245935 9.25893 190-199
TGGTT 2039480 1.6189663 17.133701 10-14
AGAAC 1321070 1.6082919 10.314149 160-169
CTCTT 1670650 1.6073353 8.900786 200-209
TGACT 1639540 1.6002266 11.70563 190-199
GGGCG 3034285 1.5860044 48.471104 20-24
TGCAG 1921210 1.579891 12.030281 150-159
GCAGA 1713395 1.5736189 12.478816 150-159
GACGT 1908105 1.5691142 9.769934 170-179
ATGGG 2073710 1.5489913 52.31275 15-19
CTTGA 1585245 1.5472335 9.323969 200-209
CACTG 1703635 1.5423399 6.4543734 230-239
GAGAA 1385355 1.5319648 10.087258 160-169
CAAAG 1258365 1.5319537 24.889166 70-79
AACTG 1401415 1.5276225 9.918081 160-169
CCACC 1645960 1.521662 7.1973333 230-239
CGACT 1667910 1.5099974 9.023055 190-199
CTGGT 2047755 1.5077888 6.926495 160-169
GGACG 2173505 1.5059329 7.595623 170-179
ACTGA 1376740 1.5007253 7.068765 180-189
GTCCC 1980160 1.4888744 15.661279 50-59
ATAAA 852225 1.4826759 5.910907 110-119
GAGGG 2326910 1.4644458 9.310239 200-209
TCGAC 1616150 1.4631377 9.608256 190-199
AGCTC 1614550 1.4616891 9.054613 140-149
CAAAA 898795 1.4504297 16.60893 70-79
AACCA 1080440 1.4480748 10.657135 70-79
GCTCT 1774050 1.4380687 8.6953945 130-139
TGGAC 1745560 1.4354465 10.462285 170-179
ACGGA 1543935 1.4179829 9.124705 200-209
TCCAC 1419700 1.4149836 9.740642 240-247
TCACG 1562700 1.4147482 8.320598 200-209
CTGAA 1288845 1.4049146 11.649155 35-39
GGAAT 1414110 1.4001712 8.053476 170-179
TCAGA 1280670 1.3960035 8.297671 130-139
CCTCG 1849675 1.3907633 8.412567 120-129
CAGAC 1373995 1.3892438 10.9154 60-69
TAGTT 1317020 1.385823 8.6627 220-229
GAAGA 1250205 1.3825121 15.827931 35-39
CGTAG 1673050 1.3758187 7.8280234 210-219
CTCGA 1514480 1.3710935 8.803981 190-199
GAGGC 1976310 1.3693047 22.617287 30-34
GGCCG 2369525 1.3635172 11.137871 120-129
GGGAG 2157890 1.3580728 6.691513 150-159
TCCAG 1471430 1.3321193 5.1425247 240-247
AGCCG 1742220 1.3289214 9.718258 120-129
AGATC 1212445 1.3216342 6.43048 140-149
AATTG 1123005 1.3197355 8.719075 170-179
GGCTC 1925125 1.3148175 7.871522 130-139
TATGG 1481575 1.3135053 6.7750697 180-189
GGTGG 2327710 1.311695 38.92423 25-29
CCATC 1315210 1.3108407 9.776182 240-247
ATCCA 1092115 1.3105962 15.253212 240-247
AAGAG 1169120 1.2928461 11.059072 35-39
GTGTT 1627770 1.2921455 7.242657 160-169
AGAGC 1392075 1.2785115 7.0369678 140-149
GAATT 1086860 1.2772584 9.792698 170-179
CGGCT 1868635 1.276236 7.2231746 130-139
ACTAT 985545 1.2750667 6.7098155 230-239
CATGT 1296830 1.2657343 9.609085 70-79
CCTCA 1265805 1.2615998 5.655058 130-139
ACTCG 1383675 1.2526728 9.04117 190-199
TTCCA 1165200 1.25202 6.8375764 230-239
GTGCT 1699080 1.2510546 7.3470173 220-229
GGGCC 2162745 1.2445278 28.88175 15-19
CAATA 859760 1.2422915 6.7259617 100-109
ACTGT 1261925 1.2316662 5.1847267 230-239
ACGTG 1495185 1.2295527 9.306164 170-179
TGGGC 1980145 1.228435 31.566458 15-19
ACTTC 1141240 1.2262747 5.189554 210-219
TATAC 945250 1.2229344 5.9746733 110-119
GCTCA 1343430 1.216238 8.849086 140-149
TGATA 1033230 1.2142334 6.986611 180-189
AAGTA 922415 1.2106575 15.530662 70-79
ACCAT 1007265 1.2087716 9.027466 45-49
AGAGA 1089145 1.2044073 11.118876 35-39
CCATG 1328100 1.2023594 9.600753 70-79
TTGAG 1352595 1.1991568 8.438129 200-209
GTCCA 1321500 1.1963843 11.0336275 70-79
GCGTA 1448025 1.1907712 8.879012 210-219
GGAGT 1579720 1.1799973 6.4733353 210-219
AAAGT 895990 1.1759751 18.726673 70-79
CTGAT 1203410 1.1745543 5.8855166 180-189
TGTGC 1593780 1.1735209 5.9776645 220-229
ATATC 905565 1.1715913 5.129893 70-79
AGTTC 1179305 1.1510273 5.5626307 160-169
CTTCT 1194675 1.1493989 5.0518885 210-219
TAGAT 973510 1.1440516 7.677531 240-247
TATCA 883160 1.1426042 5.1172724 70-79
ACTGG 1378430 1.1335404 6.8168793 160-169
CCCAA 1016665 1.131677 8.899715 240-247
CTGCG 1625305 1.1100471 6.209759 210-219
GTCTA 1135630 1.1083995 7.5155134 180-189
TGCCC 1467770 1.1036104 9.298838 60-69
GAACC 1084160 1.0961921 7.9136705 70-79
TGAAC 1004815 1.0953057 5.041504 190-199
AGGTT 1224965 1.0860051 18.821379 10-14
AGAAA 735390 1.0779594 5.2578397 5
AAAAG 730040 1.0701172 11.143386 70-79
ACCCA 956945 1.065201 10.669416 240-247
AGACC 1040845 1.0523964 5.4173055 240-247
GCGGT 1692815 1.0501825 6.9091215 130-139
TCTGT 1201045 1.0496125 5.1523056 210-219
GTGGT 1561025 1.0440493 7.3738017 50-59
GCATA 952510 1.0382903 6.85301 210-219
TGGTC 1398630 1.0298294 8.304624 50-59
TATGT 977800 1.0288817 6.9050264 220-229
CCAAT 855775 1.0269755 10.131572 240-247
TCTAC 954805 1.0259483 7.042538 180-189
GGAAG 1225520 1.022377 9.586671 30-34
ACAAT 702450 1.0149897 7.5455174 100-109
CTCGG 1461520 0.99818563 6.787569 130-139
ACGTC 1099240 0.9951672 7.2070765 70-79
ATAAC 678375 0.9802032 6.231233 80-89
GTATG 1101345 0.9764085 5.254768 220-229
TATAA 626790 0.9763921 6.5485053 240-247
CCAGC 1162110 0.9758764 5.750363 80-89
TACTG 991405 0.96763283 5.1096377 180-189
GCTTC 1190870 0.9653352 5.427956 150-159
CGGTC 1410415 0.96328205 6.898411 130-139
TGTAA 817280 0.96045285 6.505955 230-239
TCCCC 1156690 0.9574721 5.4550314 90-99
AGCTT 974755 0.95138204 5.7278724 150-159
CGATG 1142130 0.939221 22.58165 15-19
CGGAG 1325000 0.91803855 5.903108 210-219
ACTAA 628090 0.9075449 10.360109 240-247
CATCA 756115 0.9073783 5.1510925 230-239
ACCGT 997025 0.9026296 12.345673 45-49
TGAAG 889615 0.8808461 8.392987 35-39
AATCC 719120 0.86298233 9.937429 240-247
GACCG 1118885 0.8534573 12.514305 45-49
AGCTG 1022015 0.84044546 6.267134 30-34
TGCAT 855910 0.83538675 5.7439184 210-219
CTACC 835775 0.8329985 6.0881176 230-239
GAGCT 1010150 0.8306883 5.255323 140-149
TGCGT 1119460 0.82427305 6.7820954 210-219
AAAGG 744155 0.8229076 6.9570546 10-14
CCTTT 851975 0.81968665 6.1461015 50-59
ATCAA 550870 0.7959676 11.278309 70-79
AGCCA 775345 0.7839499 6.929737 80-89
TGAGA 761060 0.75355834 5.674464 240-247
TAACC 603780 0.7245682 5.8014255 80-89
TAGGT 816425 0.7238098 6.1380534 10-14
AGGAT 727985 0.7208093 8.251382 10-14
CAATC 600080 0.720128 9.964476 240-247
CTAAT 555380 0.718533 8.411918 240-247
GGTCC 1042385 0.71192575 9.043421 50-59
TGGAT 787605 0.6982591 6.6142364 10-14
ATACC 574060 0.6889026 5.712961 240-247
TCGAA 622825 0.67891484 11.422935 70-79
AAGGT 681190 0.6744756 6.9642587 10-14
ACGAT 599700 0.6537072 8.523838 10-14
CTTTG 743735 0.64996195 5.3463297 50-59
AATGG 655550 0.6490883 5.5191784 10-14
GTCGA 787875 0.6479024 7.963774 70-79
CGAAC 601025 0.6076953 7.896442 70-79
CATCC 601105 0.5991081 6.505506 240-247
CGTCC 788195 0.5926407 5.7753 70-79
AAAGC 486695 0.5925103 7.621792 70-79
GAAGC 631505 0.5799877 8.527565 30-34
TTGCC 693475 0.5621401 6.3522696 60-69
ATAGG 561180 0.5556485 5.1284447 10-14
AAGCT 497315 0.54210174 13.192374 30-34
TCAAG 483735 0.52729875 5.1969047 70-79
CGGTT 681695 0.5019409 9.95018 10-14
TAAAG 379860 0.49856123 6.271658 9
GGGAT 665545 0.49713957 6.0730495 10-14
GACGA 522065 0.47947577 5.565757 40-44
TCGAT 449925 0.4391366 6.4937534 10-14
GCGAT 395495 0.325232 5.18047 10-14