FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_7_SL254634.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_7_SL254634.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12707171
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14651705 8.338059 58.347538 110-119
CACAG 19189105 7.1740704 41.01641 110-119
CAGTA 16396610 6.4842763 42.22735 60-69
TCCCT 20443960 6.4320917 92.46167 30-34
ATACA 11745780 6.3192015 32.1056 110-119
GGAGA 20614140 6.1819215 93.39788 35-39
AGTAA 12182380 5.869973 43.22667 110-119
GGTGA 22295680 5.804689 92.39906 45-49
CCCTG 20592055 5.485473 55.162525 30-34
GTAAT 13070980 5.467793 37.26566 110-119
ACAGT 12672855 5.011663 33.840115 110-119
AGACG 14930485 4.999292 99.9768 40-44
GGCCC 21919795 4.943995 57.466766 60-69
GTAGT 15989535 4.9166226 24.71447 70-79
GTGAC 16743405 4.8671904 83.05518 45-49
CCCCA 13952425 4.780138 101.272705 60-69
GAGAC 14241875 4.768719 130.16429 40-44
ACCAG 11906270 4.4512978 97.134964 45-49
GTGTC 17587930 4.438634 25.262684 120-129
AATAC 8195020 4.4089003 32.075962 110-119
CGTGT 16974800 4.2838984 24.279324 120-129
TGACC 13025500 4.227711 152.22462 45-49
GCACA 11287590 4.219997 33.679523 110-119
CCCTT 13407170 4.2181726 64.600174 20-24
ACTCC 11492545 4.164889 90.99985 30-34
TAGTA 9906500 4.144043 27.641993 80-89
GACCA 10823615 4.0465345 108.650024 45-49
TGGTG 17643165 3.9878125 28.439009 25-29
CTCAG 12142585 3.9411418 30.124056 140-149
CCCAG 12818300 3.9331892 83.78254 60-69
AGTAG 11093305 3.9290907 27.383007 70-79
CCACT 10758495 3.8988698 27.802956 240-247
TGAGG 14767485 3.8447201 69.70744 35-39
CACTC 10515785 3.810912 78.16203 25-29
CTTGG 14712145 3.712877 38.011417 20-24
GACGG 15061685 3.7071083 111.96454 40-44
CTCCC 12311455 3.6618464 56.821537 30-34
AGGAG 12152445 3.6443653 92.99553 35-39
CAGGG 14803970 3.6436772 33.867405 50-59
CTGAG 12519810 3.6394207 97.67514 35-39
ACGGT 12283890 3.5708406 93.79578 40-44
GCCCC 14179155 3.5708218 74.14626 60-69
ATGGT 11610085 3.5699859 25.423439 190-199
TCTTG 11972115 3.568439 19.718943 170-179
TGTCT 11962975 3.565715 21.03974 180-189
AGATA 7230020 3.483722 18.40503 150-159
AGGCT 11857440 3.4468749 44.76011 30-34
GAGGA 11356930 3.4058003 129.50401 35-39
GCCCT 12602330 3.3571072 33.636883 20-24
TGTCC 11846745 3.3381815 19.215527 120-129
CCAGG 11991595 3.2954524 39.457905 45-49
CTTCA 8500325 3.2585115 17.058292 200-209
TCAGG 11124305 3.2337573 20.294485 140-149
CTCTC 10230570 3.2187486 13.968214 100-109
ATGCA 8109575 3.2070487 77.22189 25-29
TTCCC 10183745 3.2040164 55.618713 50-59
GATGG 12280365 3.197198 33.07384 15-19
CCGTG 13304185 3.1741416 24.27086 120-129
GGGTT 14013765 3.167474 42.861374 50-59
CCTGG 13236885 3.158085 31.831553 50-59
ACAGA 6900405 3.1432722 14.879 140-149
TCGCA 9494730 3.081722 24.993341 100-109
CGCAC 9823725 3.0143282 25.354816 100-109
TGGAG 11497550 2.9933915 32.002342 25-29
GGATG 11468850 2.9859197 55.826195 20-24
GACTC 9194135 2.9841578 21.363607 190-199
TCTCA 7768535 2.9779875 20.828213 130-139
GGTTC 11757300 2.9671683 48.40528 50-59
TACAC 6715420 2.96522 22.87317 110-119
TGCAC 9071715 2.9444234 100.23589 25-29
CCAGT 9070210 2.9439352 41.41226 60-69
TCTCT 8814965 2.933623 15.898641 180-189
TGGCC 12121340 2.891936 38.798897 60-69
TTCTT 8161645 2.8731475 21.867392 170-179
CAGAT 7252795 2.8682225 15.708453 150-159
AGGGT 10688405 2.7827303 50.873177 50-59
CCTGA 8570680 2.7818017 65.49476 30-34
GTTCC 9848445 2.7750995 55.12664 50-59
GCCGT 11616790 2.7715592 23.099285 120-129
TCACA 6243305 2.7567558 12.555828 130-139
CGGTG 12820015 2.7393672 67.850876 40-44
AATAA 4155865 2.7241952 13.615551 110-119
TCAAA 5045435 2.7144318 56.09269 70-79
CGGAT 9311710 2.7068486 63.096043 20-24
GCACT 8323910 2.7017071 70.78463 25-29
ACCAC 6444625 2.690202 15.604043 230-239
AGGGA 8953210 2.6849551 10.103772 150-159
GGTTG 11853240 2.6791394 56.954597 15-19
CGGCC 11673570 2.6329656 10.984376 120-129
GGCTG 12211815 2.609408 24.870337 30-34
AGTCA 6528435 2.581764 29.650944 70-79
AGATG 7247590 2.5669932 17.1779 180-189
CTGGC 10341850 2.4673812 34.67265 60-69
TGTTC 8266480 2.463928 12.798795 140-149
GAGAT 6937090 2.4570186 16.684824 180-189
GTTGG 10868435 2.456548 44.54775 15-19
GATGC 8290860 2.410095 72.89647 25-29
ACTCT 6214150 2.3821301 13.244437 200-209
GTCTC 8432135 2.376011 12.729474 180-189
CAGAG 7069205 2.3670375 9.635369 140-149
ACAGC 6312245 2.359906 12.611865 120-129
GTTCT 7892245 2.352383 18.466911 160-169
TTGTC 7873245 2.3467193 15.245507 170-179
TCATT 5755295 2.3337152 16.465076 150-159
CATTT 5708385 2.3146935 17.72234 150-159
CTGGA 7906195 2.2982752 15.33667 180-189
TTCAT 5662585 2.2961223 17.189255 140-149
TCCAT 5958915 2.2842886 12.933429 70-79
TGTAG 7404865 2.2769222 8.949512 210-219
TCCTC 7200960 2.2655706 15.017571 130-139
ATATA 3953155 2.2496796 10.53182 110-119
TTGGT 8358280 2.2312486 41.309124 25-29
CACTA 5018440 2.2159119 15.088359 240-247
GTGAA 6198825 2.1955354 17.285852 190-199
AATAT 3844180 2.1876636 12.734902 110-119
GGCGG 12087510 2.1868837 35.371254 20-24
TAGTC 6349725 2.1800294 25.025814 70-79
TTCAC 5684440 2.1790712 17.415411 200-209
ATTTG 5882125 2.1361816 15.654216 150-159
TGAAT 5091310 2.1297736 14.883068 190-199
ACTAC 4818740 2.1277335 12.269877 230-239
TTGGA 6915325 2.1263938 17.26743 170-179
GCGGA 8591255 2.1145518 75.86715 20-24
GAATC 5331715 2.1085038 15.1776285 190-199
CAGCC 6862330 2.105649 11.585966 120-129
GAGTC 7220735 2.0990171 12.60569 210-219
CTCTG 7433705 2.0946727 15.235936 180-189
GATAC 5255515 2.0783694 13.2830105 150-159
CTCGC 7788190 2.074679 17.364893 100-109
ATATG 4939920 2.0664454 9.919871 180-189
GTCAA 5207575 2.0594106 40.31097 70-79
TCTGC 7300280 2.0570765 10.722955 210-219
CCTTG 7286000 2.0530524 42.89564 20-24
TCGGC 8604865 2.0529675 8.703639 190-199
CTACT 5337990 2.0462635 8.175492 230-239
TCTCG 7238320 2.039617 16.838572 100-109
GTTCA 5903770 2.026921 15.257203 140-149
AATAG 4205175 2.0262265 8.247906 240-247
CCTTC 6417065 2.0189412 13.887886 200-209
AATCG 5079860 2.0089037 13.852532 190-199
ATAGA 4166250 2.007471 10.306647 240-247
GATAT 4796980 2.006651 9.836685 180-189
ACACA 3942830 2.0053573 14.2094345 110-119
TACAG 4991290 1.9738774 11.919422 160-169
CACGG 7137545 1.9614938 7.4277105 200-209
TCCCA 5384615 1.9513803 12.046891 240-247
TTGGG 8576305 1.9384671 70.63824 15-19
GCTGA 6666090 1.9377854 39.304485 30-34
GTTGT 7202885 1.9228152 7.87592 210-219
TTGCA 5593145 1.9202753 13.759073 150-159
GGTCA 6602895 1.9194152 10.185887 130-139
TTTGC 6357525 1.89494 11.99202 150-159
TACTA 4053615 1.8933145 10.701064 240-247
TGGAA 5334315 1.8893383 9.545093 25-29
AGTAT 4514930 1.8886651 12.141477 220-229
ATTGT 5199215 1.8881726 8.611354 170-179
GCTGT 7467715 1.8846132 10.055394 140-149
GTCCT 6646465 1.872844 12.856228 130-139
GTCAC 5753370 1.8673819 9.486783 130-139
TACCA 4218280 1.8625981 11.92186 230-239
GAACT 4687525 1.8537495 11.179643 160-169
AAAAA 2447755 1.848182 6.2252035 230-239
CAGGC 6669720 1.832929 14.136402 140-149
AGTCT 5326195 1.8286242 13.063819 210-219
CATAG 4566935 1.8060602 8.047593 210-219
CTGTT 5982115 1.7830443 12.471762 140-149
ATGTA 4260785 1.7823526 10.923526 70-79
CACCA 4240195 1.7699991 11.557501 230-239
TTGTA 4856945 1.7638721 10.420779 210-219
ATAGT 4192960 1.75398 7.433454 220-229
AGAAC 3841095 1.7496954 11.193065 160-169
TCAGC 5381130 1.7465636 11.556725 140-149
GTCTG 6846540 1.7278484 8.94776 210-219
CCAGA 4614295 1.7251079 23.662169 60-69
TCTGG 6784515 1.7121955 12.342986 180-189
GTGGA 6571290 1.710838 39.55959 25-29
TGGGG 8935180 1.7099708 42.17926 15-19
GCAGA 5097545 1.7068511 13.738109 150-159
CAGCT 5193355 1.6856171 9.41923 150-159
GACGT 5793825 1.6842242 10.406041 170-179
CCCAC 4880445 1.6720535 10.926102 240-247
ATAAT 2926005 1.6651444 5.873837 110-119
TTCAG 4818240 1.6542298 10.341662 150-159
ACGGC 6017585 1.6537136 12.34843 120-129
TGACT 4816725 1.6537097 12.195816 190-199
GAGAA 4048640 1.6517347 10.974874 160-169
GGACG 6704385 1.6501396 8.176458 170-179
GGAGG 7482755 1.6494795 27.189724 30-34
TGCAG 5661280 1.6456944 12.986146 150-159
ATCTC 4282640 1.6417058 7.1302533 100-109
CTCCA 4500245 1.6308852 8.514614 240-247
AACCA 3180550 1.6176552 12.386141 70-79
AACTG 4084785 1.6153871 10.870082 160-169
GTCCC 5966865 1.5895001 17.108488 50-59
CGACT 4892570 1.5879906 9.9724045 190-199
ATAAA 2417285 1.5845453 6.892696 110-119
GGGGC 8746650 1.5824523 38.95127 15-19
CAAAA 2543825 1.5764037 20.501198 70-79
ACGGA 4705965 1.5757353 9.963114 200-209
CACTG 4836655 1.5698422 6.783019 230-239
TCGAC 4803755 1.5591637 10.338008 190-199
CAAAG 3410790 1.5536827 28.571629 70-79
TGGTT 5818930 1.5533675 16.064066 10-14
ATGGG 5924785 1.54252 54.211555 15-19
AGCTC 4751735 1.5422796 9.700682 140-149
CTCTT 4625300 1.5393013 9.385431 200-209
ACTGA 3882265 1.5352976 7.5324373 180-189
CCTCG 5711170 1.5213863 9.3284445 130-139
GGGCG 8382560 1.5165806 45.657555 20-24
AAATA 2265155 1.4848231 5.173812 110-119
TCAGA 3735625 1.4773068 8.810864 130-139
AGCCG 5363420 1.4739403 11.163464 120-129
GCTCT 5204955 1.4666545 9.409496 130-139
TGGAC 5043920 1.4662321 11.385267 170-179
CTGGT 5808135 1.4657881 7.1479206 160-169
TCCAC 4031320 1.460947 10.483324 240-247
CTCGA 4494735 1.4588646 9.577573 190-199
CCACC 4229885 1.4491699 8.383545 230-239
AATTG 3460465 1.447566 9.383273 170-179
GAGGG 6554185 1.4447879 9.71123 200-209
ATCGG 4928025 1.4325422 9.501482 190-199
GGCCG 7031705 1.4204496 11.7747755 120-129
CCATC 3917750 1.4197894 11.062284 240-247
ACACG 3783030 1.4143296 9.114116 120-129
TAGTT 3861205 1.4022543 8.882426 220-229
CGTAG 4793650 1.3934804 8.189863 210-219
CAATA 2579560 1.3877969 7.75633 100-109
CTTGA 4020085 1.3802019 9.792378 200-209
ACTAT 2953130 1.379313 7.5239897 230-239
GGGAG 6233170 1.3740242 7.103695 150-159
GAATT 3269390 1.3676364 10.410499 170-179
TATGG 4436515 1.3641841 7.1721754 180-189
CAGAC 3585670 1.3405445 12.202807 60-69
CATGT 3881095 1.3324829 10.463853 70-79
GGCTC 5550585 1.3242708 8.397761 130-139
ACTCG 4077550 1.3234581 9.751695 190-199
GAGGC 5373850 1.322657 23.859316 30-34
ACGTG 4522045 1.3145267 9.859238 170-179
ACCAT 2974830 1.3135478 10.361837 45-49
TATAC 2805125 1.3101846 6.440515 110-119
AAGTA 2695295 1.2987041 17.392878 70-79
GTGCT 5116045 1.2911268 7.52651 220-229
ACTTC 3347665 1.2832927 5.726166 220-229
ATCCA 2900555 1.2807515 16.48733 240-247
TTCCA 3335905 1.2787846 7.5898266 230-239
GGTGG 6663505 1.2752289 39.232933 25-29
GGGCC 6281725 1.2689488 30.549603 15-19
ACTGT 3689990 1.2668715 5.2987094 230-239
CCATG 3893615 1.263758 10.554597 70-79
TGATA 3005975 1.2574458 7.0736136 180-189
GCTCA 3870650 1.256304 9.577324 140-149
AAAGT 2596660 1.2511779 21.032747 70-79
GTGTT 4680530 1.2494707 7.2923 160-169
GTCCA 3833415 1.2442186 11.929622 70-79
GAACC 3305955 1.2359698 9.237412 70-79
TTGAG 4015125 1.2346109 8.912487 200-209
TGTGC 4879685 1.2314773 6.368322 220-229
TATCA 2635450 1.2309347 5.708072 70-79
AGAGA 3009920 1.2279652 12.64803 35-39
TGGGC 5742830 1.2271218 32.685062 15-19
CCTCA 3375475 1.2232695 5.765857 130-139
GGAGT 4695520 1.2224804 6.853207 210-219
AGTTC 3483545 1.1959935 5.8414664 160-169
GGAAT 3373305 1.1947767 8.33298 170-179
GCGTA 4083975 1.1871828 9.052404 210-219
CTGAT 3441300 1.1814896 6.059793 180-189
ACTGG 4022315 1.1692587 7.213664 160-169
TGCCC 4372840 1.1648712 10.059204 60-69
CTGCG 4881170 1.1645602 6.2904577 210-219
GTCTA 3388925 1.1635078 7.7829237 180-189
ACAAT 2153130 1.1583786 8.9461155 100-109
TCACG 3542505 1.1497976 8.594182 200-209
AGATC 2871330 1.1355089 5.284331 130-139
AGTGA 3202160 1.1341594 5.040743 160-169
TATGT 3100530 1.1260039 7.530811 220-229
GCATA 2835015 1.1211476 7.4481854 210-219
CCCAA 2667790 1.1136248 9.9942665 240-247
TAGAT 2656385 1.1112069 8.337198 240-247
CTGAA 2808030 1.1104759 13.6634035 35-39
TCTAC 2860160 1.0964128 7.383409 180-189
GCGGT 5115885 1.0931568 7.264542 130-139
CCAAT 2452770 1.0830303 11.21924 240-247
CGGCT 4521215 1.0786813 7.6923404 130-139
TATAA 1889440 1.0752511 7.2967887 240-247
CTCGG 4488375 1.0708464 7.628309 130-139
TTGGC 4227635 1.0669205 5.0588183 170-179
AGGTT 3467615 1.0662572 17.882664 10-14
TGGTC 4221700 1.0654228 8.648876 50-59
AAAAG 1894730 1.0516016 13.280504 70-79
ATAAC 1940185 1.0438148 7.2195773 80-89
GAAGA 2551755 1.0410464 17.92105 35-39
GTGGT 4584030 1.0361096 7.5997257 50-59
AGAGC 3088570 1.0341702 6.5487833 140-149
CGGTC 4261840 1.0167992 7.4369473 130-139
TACTG 2887805 0.9914602 5.2219334 180-189
CGGAG 3994980 0.983278 6.496504 210-219
CGATG 3379070 0.9822719 22.650343 15-19
CATCA 2221030 0.9807045 5.711859 230-239
GCTTC 3446525 0.9711634 5.691022 150-159
AGCTT 2823520 0.96938944 5.930845 150-159
ATATC 2070490 0.9670601 5.6956735 70-79
GAGTT 3143840 0.9666996 5.3116865 160-169
TGTAA 2307695 0.96534455 6.5401516 230-239
TCTGT 3231095 0.9630685 5.3100166 210-219
GTCAG 3305190 0.9607955 5.0877557 130-139
ACCGT 2943880 0.95550066 13.544663 45-49
AAGAG 2341970 0.9554598 12.404522 35-39
ACCCA 2274935 0.9496339 11.654156 240-247
ACTAA 1739005 0.93558043 11.564894 240-247
TAGAC 2339415 0.92515546 5.007597 110-119
TCCAG 2819420 0.91510445 5.6880765 240-247
TGAAG 2580025 0.91380805 9.641955 35-39
CTTCC 2895075 0.91085035 5.3945208 230-239
CTACC 2499090 0.9056682 6.7552357 230-239
ATCAA 1608745 0.86550087 12.60788 70-79
CCAGC 2817745 0.8646017 5.8809342 80-89
GAGCT 2963495 0.86146724 5.600371 140-149
TGCAT 2486530 0.8536917 6.0910397 210-219
AGCTG 2918895 0.8485023 7.5544753 30-34
TGCGT 3355170 0.8467379 6.8037834 210-219
AATCC 1902255 0.8399482 10.621657 240-247
CCTTT 2466295 0.82078373 6.3912344 50-59
CACAA 1607240 0.8174561 5.198552 100-109
AGCCA 2150975 0.80416703 7.4240947 80-89
TGAAC 2012335 0.7958068 5.469253 190-199
GTATG 2546055 0.78288656 5.127535 220-229
GGAAG 2584410 0.77503204 10.086115 30-34
TAACC 1752390 0.77377474 6.366506 80-89
AGACC 2044920 0.7645172 5.3942065 240-247
CAATC 1721735 0.76023877 10.595073 240-247
ACGTC 2310515 0.74992824 7.9374385 70-79
CTAAT 1605240 0.7497565 9.00041 240-247
TCGAA 1894755 0.7493081 13.28757 70-79
CGGCG 3697765 0.74697226 5.2221365 130-139
TGAGA 2108575 0.7468272 5.95161 240-247
AGGAT 2105410 0.74570626 8.582896 10-14
AAATG 1546360 0.7450999 5.3753037 9
AAAGG 1809265 0.73813075 7.2451186 10-14
TAGGT 2400130 0.7380161 6.051957 10-14
GCGGC 3619375 0.731137 5.394091 130-139
GAAGT 2041130 0.7229392 5.1857853 70-79
ATACC 1620655 0.71560663 6.0232654 240-247
GGTCC 2977735 0.71043456 9.638122 50-59
GTCGA 2397045 0.69680405 8.869961 70-79
CGAAC 1840300 0.6880176 9.128145 70-79
ACGAT 1734255 0.6858362 8.940113 10-14
TGGAT 2215005 0.68109196 7.1188183 10-14
GACCG 2456795 0.67516047 13.653018 45-49
AATGG 1869610 0.6621892 5.630792 10-14
CTTTG 2186615 0.651748 5.568367 50-59
CGTGG 3002970 0.6416714 5.128978 50-59
AAAGC 1403880 0.6394954 9.053703 70-79
AAGGT 1777475 0.62955624 6.5301023 10-14
CGTCC 2261715 0.6024933 6.257126 70-79
CATCC 1662185 0.6023745 7.310595 240-247
GAAGC 1781965 0.5966694 9.193938 30-34
TTGCC 2078275 0.5856173 6.718374 60-69
ATAGG 1651085 0.5847908 5.404784 10-14
AAGCT 1427605 0.56456697 15.272081 30-34
TCAAG 1363750 0.53931457 5.776784 70-79
GACGA 1555090 0.520703 6.666465 40-44
GGGAT 1844605 0.48024362 6.08932 10-14
CGGTT 1875875 0.4734111 9.766224 10-14
AAGCA 1021135 0.46514735 5.636235 70-79
TCGAT 1271550 0.43655685 7.085817 10-14
GCGAT 1132675 0.32926068 5.6542406 10-14