FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_BC_X.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_BC_X.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16513022
Filtered Sequences 0
Sequence length 251
%GC 53

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14526090 6.2526608 41.946564 110-119
CACAG 18286950 5.828108 33.045467 110-119
TCCCT 19803840 5.5938773 78.67081 30-34
CAGTA 16099595 5.2915654 33.106148 60-69
GGAGA 19736750 5.1653814 77.58527 35-39
CCCTG 20055945 4.9778743 48.75646 30-34
GGTGA 21172605 4.9659204 78.46164 45-49
ATACA 11482555 4.7926064 23.110487 110-119
AGTAA 12176750 4.605585 32.21213 110-119
GGCCC 21015440 4.583282 52.991673 60-69
GTAAT 12812330 4.3429046 28.607365 110-119
AGACG 14794030 4.272607 82.46828 40-44
CCCCA 13876250 4.2408605 88.25212 60-69
GTGAC 16036490 4.1506352 70.12326 45-49
ACAGT 12424470 4.0836368 26.838385 110-119
GAGAC 14069755 4.0634327 108.23488 40-44
GTAGT 15195385 4.056018 20.371145 70-79
GTGTC 16698155 3.8732228 21.524784 120-129
ACCAG 12093250 3.8541563 78.73521 45-49
TGACC 12970605 3.7046332 127.21934 45-49
CGTGT 15961565 3.7023666 20.60914 120-129
CCCTT 12835575 3.6255918 55.45952 20-24
TGGTG 17187890 3.612824 25.35877 25-29
GACCA 11232805 3.5799294 88.07954 45-49
ACTCC 11264885 3.5505164 76.52908 30-34
GCACA 11097985 3.5369618 27.84014 110-119
CCCAG 12630805 3.4981072 72.850914 60-69
AATAC 8315605 3.47078 23.321676 110-119
CTCAG 11921745 3.4050603 25.025253 140-149
TGAGG 14448625 3.388847 60.403168 35-39
GCCCC 14061695 3.384196 68.711464 60-69
GACGG 14845985 3.3763819 97.58764 40-44
CTCCC 12180155 3.3360562 50.177002 30-34
CTTGG 14374310 3.3341951 33.215595 20-24
CACTC 10513345 3.3136425 65.14178 25-29
AGTAG 11040585 3.2883763 22.327608 70-79
TAGTA 9634130 3.2656126 21.010975 80-89
CAGGG 14333480 3.2598243 29.583189 50-59
CTGAG 12543425 3.2465444 83.86447 35-39
CCACT 10277080 3.2391756 21.862144 240-247
AGGAG 12208635 3.1951692 76.98796 35-39
ACGGT 12231345 3.1657708 78.76106 40-44
TGTCT 11727575 3.0958145 18.019547 180-189
ATGGT 11541770 3.0807793 20.602755 190-199
TCTTG 11645700 3.0742013 16.875061 170-179
AGGCT 11740990 3.0388548 38.297848 30-34
AAAAA 5664670 3.0359411 7.769208 230-239
GCCCT 12194035 3.0265524 30.43636 20-24
GAGGA 11339030 2.9675815 108.14953 35-39
TGTCC 11519325 2.9485655 16.357138 120-129
CCAGG 11657300 2.9256358 34.01145 50-59
CTCTC 10218785 2.8864417 11.995067 100-109
GATGG 12296885 2.8841681 29.156282 15-19
CCGTG 12742895 2.8660855 21.32397 120-129
TTCCC 10146540 2.8660347 48.09932 50-59
CCTGG 12721445 2.8612607 28.110348 50-59
GGGTT 13495465 2.8366914 37.60802 50-59
TCAGG 10932160 2.82951 16.953314 140-149
AGATA 7212015 2.7277844 14.192112 150-159
ATGCA 8296835 2.726978 61.484882 25-29
CTTCA 8384825 2.725472 13.647337 200-209
GGATG 11602080 2.7212055 47.358616 20-24
TGGCC 12015840 2.7025585 35.078514 60-69
GGTTC 11539165 2.6765687 42.217377 50-59
TGGAG 11403260 2.6745734 27.031807 25-29
TCTCT 9152185 2.666068 13.750872 180-189
TCGCA 9323075 2.6628344 20.387009 100-109
TGCAC 9207170 2.62973 84.803505 25-29
GCCGT 11689725 2.62921 20.431019 120-129
CGCAC 9476405 2.6244946 21.075342 100-109
ACAGA 7047010 2.5844932 11.049487 140-149
CCAGT 9038505 2.581556 34.69161 60-69
CGGTG 12633600 2.5749474 60.862095 40-44
AGGGT 10946195 2.5673711 43.162155 50-59
TCTCA 7875745 2.5599964 16.656176 130-139
GTTCC 9844560 2.5198812 47.933876 50-59
CCTGA 8802495 2.5141475 54.814827 30-34
GACTC 8739650 2.4961982 16.177979 190-199
TTCTT 8305965 2.495281 18.020521 170-179
GGTTG 11867035 2.494402 50.978085 15-19
GGCTG 12205360 2.4876645 22.898054 30-34
TCAAA 5888800 2.4578762 42.49974 70-79
CGGCC 11189560 2.440344 10.038583 120-129
GCACT 8535710 2.4379492 59.784733 25-29
TACAC 6650365 2.4120955 17.404854 110-119
AATAA 5016225 2.4093199 10.149372 110-119
CAGAT 7318680 2.4054813 12.730188 150-159
CGGAT 9121435 2.36085 52.863396 20-24
CTGGC 10471395 2.3551877 31.353271 60-69
ACCAC 6659820 2.3422246 12.509196 230-239
TCACA 6411480 2.3254516 9.568391 140-149
GTTGG 11050585 2.3227875 39.893135 15-19
TGTTC 8642980 2.2815511 11.136199 140-149
AGTCA 6908410 2.270635 23.676796 70-79
GATGC 8590385 2.2234013 62.20927 25-29
AGATG 7392395 2.2017834 13.527423 180-189
GGCGG 12292075 2.2014244 33.83454 20-24
GTCTC 8432480 2.1584356 11.377297 180-189
AGGGA 8240980 2.1567788 7.6589255 150-159
TTGGT 8935665 2.1375377 35.17473 25-29
GAGAT 7170135 2.1355848 13.328678 180-189
CATTT 6292830 2.109486 14.824654 150-159
TTGTC 7970140 2.1039367 12.996331 170-179
TCATT 6227175 2.0874772 13.79288 150-159
GTTCT 7889905 2.0827565 14.93356 160-169
TTCAT 6177435 2.0708032 13.901054 140-149
TCCTC 7293555 2.0601687 13.305298 130-139
ACAGC 6400835 2.0399659 10.366449 120-129
CTGGA 7816955 2.0232186 12.942658 180-189
TCCAT 6185815 2.0106878 10.460022 70-79
TCTGC 7848630 2.0089893 9.472793 210-219
GTCAA 6088260 2.0010705 32.590633 70-79
CAGAG 6890220 1.9899381 7.571935 140-149
GCGGA 8746050 1.9890903 66.13308 20-24
ACTCT 6089630 1.979423 10.084134 200-209
TGTAG 7371035 1.9675087 6.9407187 210-219
CCTTG 7658230 1.9602531 36.998745 20-24
CTCGC 7851260 1.9486783 14.823272 100-109
AATAT 4526985 1.9486113 9.853304 110-119
TCGGC 8658235 1.9473784 7.671181 190-199
CTCTG 7579665 1.9401431 13.224633 180-189
TGAAT 5718465 1.9383476 11.852629 190-199
ATTTG 6356665 1.9309908 12.962288 150-159
TCTCG 7458215 1.909056 14.227433 100-109
GCTGA 7341525 1.9001658 34.53116 30-34
CAGCC 6859610 1.8997719 9.885355 120-129
TAGTC 6442350 1.8976331 20.241753 70-79
TTGGA 7083600 1.8907853 14.166595 170-179
TTCAC 5812405 1.8893116 14.081839 200-209
GTTCA 6381225 1.8796285 13.013148 140-149
CACTA 5162330 1.8723834 11.866078 240-247
GAGTC 7189930 1.8609295 10.749229 210-219
TTGGG 8846275 1.8594507 62.860645 15-19
CCTTC 6572150 1.8563976 11.701582 200-209
GAATC 5594600 1.8388158 12.31679 190-199
GTGAA 6133960 1.8269658 13.809214 190-199
ATATA 4214830 1.8142462 8.125604 110-119
CACGG 7107670 1.7838138 6.5667343 200-209
CTACT 5479085 1.7809665 6.969064 230-239
GGTCA 6875510 1.7795497 8.212575 140-149
GCTGT 7666880 1.7783719 8.95059 140-149
TTTGC 6735395 1.7779917 10.45212 150-159
AATAG 4699670 1.7775458 6.3919086 110-119
TTGCA 6033020 1.7770627 11.585241 150-159
GATAC 5394455 1.7730328 10.641694 150-159
ACTAC 4867375 1.7654028 9.780881 230-239
CAAAA 3751370 1.7471268 15.79819 70-79
GTCCT 6817370 1.7450207 11.573754 130-139
GTTGT 7263305 1.7374855 6.2510495 210-219
AACCA 4281885 1.7329469 9.910734 70-79
CTGTT 6546625 1.7281609 10.895884 140-149
TACCA 4761145 1.7268729 9.666897 230-239
TGGGG 9318765 1.7211545 39.40348 15-19
CAGGC 6847415 1.7184976 12.302595 140-149
ACACA 4225320 1.7100542 10.870677 110-119
TCAGC 5983990 1.709133 9.517761 140-149
TACAG 5184625 1.7040666 9.722968 160-169
AATCG 5145045 1.6910577 10.918096 190-199
GATAT 4975415 1.6864811 7.1888943 180-189
TGGAA 5652745 1.6836386 7.658526 170-179
ATTGT 5540290 1.6829972 7.244232 170-179
GTCAC 5864775 1.675083 7.8359594 140-149
CACCA 4758960 1.6737019 9.220703 230-239
ATATG 4930490 1.6712531 7.194926 180-189
ATAAA 3469380 1.6663619 5.3752375 110-119
GCAGA 5749120 1.6603816 11.667088 150-159
TCTGG 7115375 1.6504478 11.091701 180-189
ATAGA 4363095 1.6502438 7.3543005 240-247
TCCCA 5201870 1.6395484 9.478225 240-247
GTCTG 7037530 1.6323912 7.8051543 210-219
GGAGG 7919110 1.6320714 24.507315 30-34
GGGGC 8947555 1.602444 37.108746 15-19
AGTCT 5431300 1.5998223 10.731789 210-219
AGTAT 4716140 1.5985966 9.3404875 220-229
GTGGA 6798890 1.594643 33.75065 25-29
ACGGC 6326125 1.5876694 10.721417 120-129
CCAGA 4961125 1.5811257 19.975414 60-69
TACTA 4219945 1.5784786 8.399461 230-239
TTCAG 5333440 1.570997 8.484089 150-159
ATCTC 4831125 1.5703483 5.9389486 100-109
TGCAG 6063925 1.5694917 11.245528 150-159
CATAG 4771015 1.5681226 6.263026 210-219
TGGTT 6525495 1.5609908 14.111792 10-14
GGGCG 8589065 1.5382413 44.140915 20-24
CAGCT 5370245 1.5338365 7.7402334 150-159
ATAGT 4496435 1.5241246 5.7604833 220-229
GTCCC 6109295 1.5163236 15.752138 50-59
GAACT 4575900 1.5039927 8.3763685 160-169
CTCTT 5139135 1.4970505 7.46828 200-209
GGACG 6562800 1.4925597 6.8180118 170-179
CAAAG 4041075 1.4820656 22.735302 70-79
ATGGG 6282570 1.4735429 47.457535 15-19
CTGGT 6351485 1.4732593 6.014526 160-169
CAATA 3523955 1.4708337 6.3525352 100-109
ATGTA 4334585 1.4692636 8.600096 70-79
ACCAT 4042130 1.4660854 9.036925 45-49
CACTG 5129990 1.4652154 5.403085 230-239
AGAAC 3993995 1.4647988 8.144932 160-169
GACGT 5656780 1.4641128 8.2760935 170-179
TTGTA 4817760 1.4635113 7.430526 210-219
GAGAA 4399055 1.4620093 8.234908 160-169
CCTCG 5865995 1.4559367 8.507558 130-139
CTCCA 4615055 1.4545934 6.7568316 240-247
GCTCT 5681805 1.4543537 8.251226 130-139
CCCAC 4743145 1.4496002 9.079545 240-247
TGACT 4909725 1.4461892 9.422721 190-199
ACTGA 4363900 1.4343133 5.900493 180-189
GGCCG 7134705 1.4100494 10.894035 120-129
ACGGA 4854345 1.401965 8.160052 200-209
TCAGA 4233935 1.3915968 6.8807864 130-139
AACTG 4228995 1.389973 8.020092 160-169
CCATC 4383495 1.3816093 9.19744 240-247
CGACT 4830175 1.379583 7.4971266 200-209
AGCTC 4820560 1.3768369 7.8169065 140-149
CCACC 4499150 1.3750304 7.211555 240-247
AGCCG 5463990 1.371299 9.583501 120-129
GAGGC 5980110 1.3600401 21.13318 30-34
GGTGG 7332605 1.3543153 36.01675 25-29
GAGGG 6524165 1.3445833 7.7088246 200-209
CTTGA 4545370 1.3388664 7.6452003 200-209
TCCAC 4216565 1.3289956 8.393444 240-247
TGGAC 5112395 1.3232126 9.162835 170-179
TCGAC 4609130 1.3164487 7.9424357 190-199
ATCGG 5084085 1.3158852 8.029365 190-199
GGCTC 5784140 1.3009475 7.6197066 130-139
GGGCC 6537055 1.2919343 29.371096 15-19
TATGG 4810175 1.2839528 5.6223655 180-189
GGGAG 6188475 1.2753999 5.837873 150-159
ACACG 3993250 1.2726612 7.5056252 120-129
AATTG 3740115 1.2677602 7.5909376 170-179
CTCGA 4377865 1.2503953 7.2112517 190-199
CAGAC 3906590 1.2450423 9.989166 60-69
TGGGC 6061960 1.2355329 30.67042 15-19
TTCCA 3800630 1.2353878 6.3795943 230-239
CCATG 4319620 1.2337595 8.8701935 70-79
CCTCA 3910400 1.2324972 5.2056975 130-139
GTGCT 5309165 1.231488 6.159548 220-229
GTGTT 5100890 1.2202052 6.5221877 160-169
GCTCA 4261845 1.217258 7.861471 140-149
ACTAT 3246805 1.2144738 5.7584167 230-239
CGTAG 4686020 1.2128563 6.9060464 210-219
AGAGA 3637305 1.2088444 10.336584 35-39
GGAGT 5121585 1.2012403 6.1878343 210-219
GAATT 3536745 1.1988254 8.458772 170-179
AAAGT 3166865 1.1977965 16.598665 70-79
CTGAT 4034165 1.1882877 5.020796 180-189
AAAAG 2811320 1.1864924 10.482235 70-79
CATCA 3261360 1.1828992 5.148166 240-247
TAGTT 3893705 1.1828071 6.8440456 220-229
AAGTA 3120360 1.1802069 13.67654 70-79
TGATA 3476780 1.1784995 5.3065467 180-189
ACGTG 4539705 1.1749865 7.854291 170-179
GTCCA 4109805 1.1738327 10.110258 70-79
TGCCC 4713365 1.1698545 9.725516 60-69
TCACG 4045250 1.1553947 7.385003 200-209
CTGCG 5136120 1.1551973 5.7818427 210-219
CATGT 3903860 1.1499057 8.491454 70-79
GCGGT 5640650 1.1496626 6.372514 130-139
ACTCG 4009290 1.1451238 7.4873023 190-199
TGTGC 4935210 1.1447474 5.374492 220-229
TTGAG 4286680 1.1442192 6.7959175 200-209
GAAGA 3436300 1.1420412 15.551155 35-39
CTGAA 3468720 1.1400882 12.114614 35-39
TGGTC 4912655 1.1395156 7.5767183 50-59
ATAAC 2717055 1.1340486 5.7486944 80-89
ATCCA 3125600 1.1336588 11.951937 240-247
TATAC 3006405 1.1245517 5.245816 110-119
GCGTA 4326060 1.1196898 7.7357016 210-219
ACAAT 2679995 1.1185805 6.835718 100-109
AAGAG 3347875 1.1126535 10.327672 35-39
GAACC 3481175 1.1094612 7.4428062 70-79
ACTGG 4238445 1.097013 5.797369 160-169
GGAAT 3642840 1.0849996 7.0116563 170-179
GTGGT 5156830 1.0839444 6.6108828 50-59
CGGCT 4775615 1.074114 6.97709 130-139
GCATA 3223410 1.0594605 5.9440827 210-219
CTCGG 4709110 1.0591557 6.9908643 130-139
AGAGC 3655210 1.0556473 5.2727604 140-149
GTCTA 3562710 1.0494179 6.1415224 180-189
TATGT 3433190 1.0429145 6.0597 220-229
AGGTT 3897605 1.0403657 14.984563 10-14
CCAAT 2815390 1.0211453 8.34897 240-247
TATAA 2312225 0.99528223 5.729106 240-247
CGGAG 4373465 0.99464524 5.921037 210-219
CGGTC 4404760 0.99070245 6.26135 130-139
CCAGC 3577045 0.9906642 5.0743017 80-89
CCCAA 2790110 0.9812674 7.375206 240-247
TCTAC 3013925 0.97967076 5.6618147 180-189
ATCAA 2344835 0.9786908 10.216258 70-79
CGATG 3695855 0.95657754 19.51062 15-19
TAGAT 2771655 0.93948823 6.081289 240-247
ACCGT 3252515 0.9289756 11.47795 45-49
TAACC 2555475 0.92687386 5.5215087 80-89
TGAAG 3093670 0.92143226 8.666464 35-39
CCTTT 3111635 0.9064317 5.689372 50-59
ACTAA 2124135 0.8865747 8.767846 240-247
AGCTG 3421865 0.8856622 6.2774777 30-34
TGCGT 3774595 0.87553656 5.9991384 210-219
CTACC 2774570 0.8745012 5.768882 230-239
GGAAG 3236135 0.8469415 8.4974375 30-34
AGCCA 2653530 0.84568816 6.329461 80-89
AAAGG 2510725 0.83443 6.3052244 10-14
AAAGC 2265045 0.830706 7.3626504 70-79
TGCAT 2814835 0.82912666 5.008073 210-219
CAATC 2252675 0.81704795 7.7748065 240-247
AATCC 2228575 0.8083069 7.672225 240-247
ACCCA 2237600 0.7869525 8.898791 240-247
ATACC 2156020 0.78199106 5.0084815 240-247
CTAAT 2050535 0.76700664 7.279086 240-247
AGGAT 2565275 0.76405287 7.507072 10-14
ACGTC 2604720 0.74395394 6.5431614 70-79
TGGAT 2730215 0.7287609 6.1185207 10-14
GGTCC 3227260 0.72586346 9.101165 50-59
AAGCT 2197995 0.72243017 12.048089 30-34
GACCG 2868255 0.7198467 12.420664 45-49
GAAGC 2485510 0.71783066 7.679401 30-34
TCGAA 2172025 0.7138945 10.481185 70-79
AAGGT 2336030 0.6957734 5.5446424 10-14
CGAAC 2172770 0.6924685 7.446149 70-79
TAGGT 2583420 0.6895777 5.01771 10-14
ACGAT 2086345 0.6857335 7.328934 10-14
CGTCC 2743690 0.6809823 5.535033 70-79
GACGA 2321400 0.6704348 6.077601 40-44
TTGCC 2607090 0.66732866 6.050526 60-69
GTCGA 2536060 0.6563942 7.5514245 70-79
CATCC 2054805 0.64764255 5.933021 240-247
CGGTT 2584475 0.5994822 8.539519 10-14
GGGAT 2271345 0.5327318 5.6157722 10-14
TCGAT 1528075 0.45010373 5.7423286 10-14