FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_11_SL254621.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_11_SL254621.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4250897
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 4516005 7.4585457 52.969986 110-119
CACAG 6012450 6.453977 36.28111 110-119
TCCCT 6528270 6.1929336 87.28391 30-34
AAAAA 2871120 5.9091063 36.31567 230-239
CAGTA 5095420 5.894374 38.622818 60-69
GGAGA 6493685 5.7475247 84.50557 35-39
ATACA 3734050 5.722662 28.846329 110-119
GGTGA 7021490 5.5671577 88.29949 45-49
CCCTG 6674645 5.3352113 51.38009 30-34
AGTAA 3793570 5.279258 39.01061 110-119
GTAAT 4085530 5.0931664 35.04838 110-119
GGCCC 6957400 4.6859264 53.27259 60-69
GTAGT 4973465 4.6799183 23.967966 70-79
GAGAC 4771920 4.6513166 118.29331 40-44
AGACG 4699175 4.5804105 90.9509 40-44
GTGAC 5213515 4.552271 77.909805 45-49
ACAGT 3933560 4.550336 30.957783 110-119
CCCCA 4589590 4.509993 89.59063 60-69
ACTCC 4250465 4.501116 91.889626 30-34
GTGTC 5434360 4.2507005 24.632408 120-129
ACCAG 3856910 4.140144 85.22172 45-49
GCACA 3847325 4.1298547 30.077682 110-119
CGTGT 5241575 4.099905 23.61928 120-129
TGGTG 5648725 4.012078 28.849295 25-29
CCCTT 4221450 4.004608 61.92218 20-24
CACTC 3776410 3.9991052 76.82902 25-29
AATAC 2558915 3.9216952 28.890432 110-119
TGACC 4015980 3.8617322 139.18034 45-49
TAGTA 3046520 3.7978997 24.948435 80-89
TGAGG 4779985 3.789927 67.60061 35-39
CCCAG 4194465 3.7427034 75.33059 60-69
CCACT 3529760 3.7379105 25.011192 240-247
GACCA 3468090 3.7227705 95.40901 45-49
CTCAG 3870800 3.7221277 27.150389 140-149
AGTAG 3449300 3.6232264 26.033796 70-79
CTTGG 4583505 3.5851698 36.75492 20-24
TGTCT 3808670 3.5355768 20.234104 170-179
CTCCC 4013485 3.532956 53.94914 30-34
CTGAG 4019810 3.5099664 93.21848 35-39
CAGGG 4765210 3.5059404 32.087143 50-59
AGGAG 3929675 3.4781337 84.41137 35-39
GACGG 4676955 3.4410079 104.025894 40-44
TCTTG 3689535 3.4249842 18.72568 170-179
ATGGT 3636385 3.421756 23.804941 190-199
CTTCA 2977955 3.398471 16.000402 200-209
ACGGT 3848135 3.3600652 86.8192 40-44
GCCCC 4528615 3.358977 67.46365 60-69
AGGCT 3729910 3.2568352 42.02994 30-34
AGATA 2337415 3.2528243 16.938257 150-159
GGGTT 4574530 3.2491167 42.405556 50-59
ATGCA 2790365 3.2278898 78.420876 25-29
CCTGG 4428950 3.2146304 30.080368 50-59
GAGGA 3612045 3.197001 118.67552 35-39
TTCCC 3344850 3.1730359 52.978615 50-59
GCCCT 3964890 3.169236 32.56109 20-24
TGTCC 3650590 3.1446207 18.513062 120-129
GATGG 3900170 3.092344 32.491154 15-19
CCAGT 3214965 3.0914829 38.494007 60-69
CTCTC 3255185 3.0879767 13.088557 100-109
TCAGG 3533895 3.0856807 18.935387 140-149
GGATG 3880050 3.0763912 57.0974 20-24
CCAGG 3776980 3.0602744 36.412643 50-59
TGCAC 3123525 3.0035546 101.18786 25-29
GGTTC 3820790 2.9885824 47.17566 50-59
CCGTG 4106455 2.9805567 23.203163 120-129
TGGAG 3679485 2.917369 30.85152 25-29
ACAGA 2213715 2.858675 13.222474 140-149
GACTC 2938630 2.825761 19.865467 190-199
TACAC 2213735 2.8201778 20.714994 110-119
TCGCA 2928210 2.8157413 23.002241 100-109
TCTCT 2743265 2.8044512 14.707701 180-189
GGTTG 3948260 2.804301 62.064358 15-19
TTCTT 2534080 2.7917862 20.789581 170-179
GCCGT 3831935 2.7813036 21.962107 120-129
TCTCA 2437145 2.7812936 19.333698 130-139
GCACT 2879330 2.7687387 70.662155 25-29
CGGAT 3163790 2.7625177 63.8164 20-24
TGGCC 3789415 2.7504418 37.151264 60-69
AGTCA 2361015 2.7312183 27.320692 70-79
CAGAT 2359745 2.7297492 14.2687 150-159
AGGGT 3431285 2.7205772 48.53034 50-59
CGCAC 3044585 2.7166703 22.7536 100-109
GTTCC 3125925 2.6926737 53.104 50-59
CGGTG 3981170 2.6239033 64.38927 40-44
ACCAC 2205730 2.607483 13.886362 230-239
GTTGG 3656625 2.5971637 48.67532 15-19
CCTGA 2696255 2.5926955 59.940037 30-34
AGGGA 2910445 2.5760188 9.2815485 150-159
GGCTG 3847785 2.535992 23.83443 30-34
TCACA 1989150 2.5340686 11.241167 140-149
GATGC 2820940 2.4631524 75.73806 25-29
CGGCC 3637460 2.4498909 10.318573 120-129
AATAA 1327315 2.447142 11.288172 110-119
TCAAA 1566105 2.4001527 48.960533 70-79
ACTCT 2088025 2.3828747 12.386195 200-209
CTGGC 3280810 2.381285 33.507645 60-69
TGTTC 2550595 2.3677099 12.317008 140-149
AGATG 2243410 2.3565311 15.701553 180-189
GTCTC 2715525 2.339155 11.664206 180-189
TCCTC 2442035 2.3165956 13.62 130-139
GAGAT 2188250 2.29859 15.469052 180-189
TTGGT 2713160 2.287016 42.607574 25-29
GTTCT 2425780 2.2518442 17.704739 160-169
GGCGG 4038585 2.2428045 37.717216 20-24
TTGTC 2399350 2.2273097 14.518374 170-179
CAGAG 2279685 2.2220693 8.934808 140-149
CTGGA 2534505 2.2130466 14.268074 180-189
TCATT 1789535 2.2008348 15.260257 140-149
CATTT 1788365 2.1993961 16.208384 150-159
TTCAC 1922330 2.1937816 16.310667 200-209
TCTGC 2541475 2.1892283 10.967823 210-219
GCGGA 2974025 2.1880996 77.267395 20-24
TGTAG 2318620 2.1817691 8.377707 210-219
TTCAT 1764320 2.1698244 16.470737 140-149
CACGG 2641550 2.140299 7.2956824 200-209
GATAT 1715120 2.1381292 9.361823 180-189
TCCAT 1872225 2.1366014 11.751235 70-79
TCTCG 2470715 2.1282754 16.115345 100-109
GAACT 1823515 2.1094394 12.984604 160-169
TTGGA 2217870 2.0869653 16.28489 170-179
TTGGG 2928630 2.080096 75.72455 15-19
GTGAA 1979400 2.079209 15.766226 190-199
CTCTG 2413660 2.0791283 14.08825 180-189
ACAGC 1932550 2.0744677 10.889112 120-129
TAGTC 1999420 2.0719361 24.103397 70-79
TGAAT 1646210 2.0522237 13.965899 190-199
ATTTG 1828655 2.0421417 14.461107 150-159
CACTA 1602900 2.0420072 13.3137 240-247
ATATA 1230195 2.0317662 9.765053 110-119
GTTCA 1959305 2.0303662 14.847896 140-149
GTCAC 2110355 2.029299 9.135987 140-149
GTCTG 2552290 1.9963746 8.676264 210-219
GATAC 1720835 1.9906592 12.30522 150-159
CCTTG 2305795 1.9862133 41.064533 20-24
GAGTC 2273745 1.9853595 11.640853 210-219
GAATC 1709255 1.9772635 14.009258 190-199
CCTTC 2069840 1.963519 12.613007 200-209
CAGCC 2165170 1.9319719 10.135813 120-129
AATAT 1168370 1.9296571 11.315117 110-119
TCAGC 2006280 1.9292215 10.892931 140-149
ACTAC 1510510 1.9243076 11.088074 230-239
CTACT 1685520 1.9235318 7.618155 230-239
CTCCA 1813605 1.9205534 7.99835 240-247
CTCGC 2397980 1.9167656 15.985413 100-109
TCGGC 2636665 1.91375 8.232258 190-199
TTCAG 1835325 1.9018897 10.217568 150-159
GTTGT 2247315 1.8943392 7.772717 210-219
ACACA 1329940 1.891334 12.05241 110-119
ATATG 1513415 1.8866768 9.253882 180-189
ATAGA 1350685 1.8796583 9.224785 240-247
GTCAA 1618725 1.8725386 36.349316 70-79
AATAG 1339335 1.8638632 7.6952333 240-247
ACGGC 2299600 1.8632363 11.718483 120-129
CACCA 1574385 1.8611441 9.928695 230-239
TGGGG 3087275 1.8476483 44.533157 15-19
CCAGA 1719990 1.8462983 20.850014 60-69
TTGCA 1765945 1.8299932 12.726205 150-159
TTTGC 1970830 1.8295158 11.270445 150-159
AATCG 1578275 1.825746 12.685373 190-199
TCCCA 1722360 1.8239276 10.153727 240-247
TACAG 1568415 1.81434 10.565272 160-169
GCTGT 2317345 1.812603 9.560605 140-149
GCTGA 2066670 1.8045485 35.540062 30-34
GGTCA 2064240 1.8024267 9.372444 130-139
ATCTC 1577665 1.8004467 6.6434216 100-109
GTCCT 2072465 1.7852226 11.734295 130-139
AGTCT 1711060 1.7731178 12.33505 210-219
TGGAA 1676115 1.7606312 8.506729 170-179
ATTGT 1562460 1.7448698 8.084982 170-179
AGTAT 1388650 1.7311403 10.646246 220-229
TACTA 1253845 1.7213789 9.58501 230-239
CTGTT 1848090 1.7155763 12.007133 140-149
TACCA 1346445 1.7152977 10.922237 230-239
CATAG 1476790 1.7083484 7.4718065 210-219
CAGGC 2108285 1.7082245 12.687325 140-149
TTGTA 1516715 1.6937842 9.71196 210-219
GTGGA 2130185 1.6889688 37.746353 25-29
GGGGC 3013980 1.673796 40.286804 15-19
GGAGG 2502340 1.6717666 26.904684 30-34
ATGTA 1333280 1.662114 10.112297 70-79
CCCAC 1689520 1.6602187 9.564907 240-247
ACACG 1538875 1.651883 8.945787 110-119
TCTGG 2105670 1.6470331 11.691552 180-189
ATCGG 1869840 1.6326829 9.299059 190-199
ATAGT 1303945 1.6255441 6.43015 220-229
CAGCT 1685915 1.6211612 8.76393 150-159
TGGTT 1918350 1.6170434 17.224663 10-14
CTCTT 1577810 1.6130017 8.933391 200-209
AGAAC 1243065 1.6052287 10.274286 160-169
TGACT 1547775 1.6039106 11.730291 190-199
GGGCG 2857585 1.586943 48.45497 20-24
TGCAG 1806875 1.577704 12.037235 150-159
GCAGA 1612090 1.5713468 12.444734 150-159
GACGT 1796360 1.5685227 9.783485 170-179
ATGGG 1956410 1.551187 52.482132 15-19
CTTGA 1492805 1.5469468 9.3456 200-209
CACTG 1600670 1.5391904 6.4464264 230-239
CAAAG 1186660 1.5323904 24.891386 70-79
GAGAA 1305875 1.5312692 10.061118 160-169
AACTG 1318025 1.5246892 9.935825 160-169
CCACC 1542350 1.5156009 7.229908 230-239
CGACT 1573080 1.5126601 9.084563 190-199
GGACG 2053440 1.5107914 7.623015 170-179
CTGGT 1923490 1.5045338 6.932972 160-169
ACTGA 1298180 1.5017326 7.05116 180-189
ATAAA 809775 1.4929646 5.952289 110-119
GTCCC 1853775 1.4817688 15.680089 50-59
TCGAC 1523855 1.4653257 9.632547 190-199
GAGGG 2192220 1.4645813 9.315712 200-209
AGCTC 1519505 1.4611429 9.02151 140-149
AACCA 1021770 1.4530792 10.652346 70-79
CAAAA 849255 1.4529192 16.637886 70-79
GCTCT 1670965 1.4393703 8.665334 130-139
TGGAC 1643050 1.4346575 10.520896 170-179
ACGGA 1458040 1.4211903 9.1743 200-209
TCACG 1475865 1.419179 8.392162 200-209
TCCAC 1337710 1.4165949 9.728045 240-247
CTGAA 1209340 1.3989626 11.551488 35-39
GGAAT 1331595 1.3987391 8.074658 170-179
CCTCG 1743715 1.3937951 8.504965 120-129
TCAGA 1202945 1.3915648 8.320225 130-139
CAGAC 1294745 1.3898251 10.901053 60-69
TAGTT 1238225 1.3827819 8.587784 220-229
CGTAG 1577115 1.3770851 7.8474293 210-219
GAAGA 1174100 1.3767498 15.899911 35-39
CTCGA 1429910 1.374989 8.790318 190-199
GAGGC 1858500 1.3673668 22.546257 30-34
GGCCG 2232650 1.3654506 11.207131 120-129
GGGAG 2029125 1.3556205 6.660939 150-159
AGCCG 1652485 1.3389155 9.779679 120-129
TCCAG 1381410 1.3283519 5.1631746 240-247
AGATC 1141960 1.3210175 6.3794026 140-149
GGCTC 1815770 1.3179263 7.8420553 130-139
AATTG 1055970 1.3164096 8.69644 170-179
TATGG 1395270 1.3129176 6.796089 180-189
GGTGG 2193480 1.3127366 39.05422 25-29
CCATC 1237280 1.3102427 9.709738 240-247
ATCCA 1023580 1.3039852 15.257462 240-247
GTGTT 1533855 1.2929393 7.253874 160-169
AAGAG 1100600 1.2905636 11.08352 35-39
CGGCT 1762955 1.2795919 7.217829 130-139
AGAGC 1309785 1.2766821 7.0846496 140-149
GAATT 1022210 1.2743232 9.750638 170-179
ACTAT 927190 1.2729207 6.7275987 230-239
CATGT 1220645 1.2649161 9.630508 70-79
CCTCA 1191820 1.2621018 5.670986 130-139
TTCCA 1099410 1.2546574 6.8576083 230-239
ACTCG 1303695 1.253622 9.058786 190-199
GTGCT 1595975 1.2483552 7.332071 220-229
GGGCC 2038915 1.2469656 28.993074 15-19
CAATA 812715 1.245536 6.7189026 100-109
ACTGT 1194565 1.2378902 5.218355 230-239
ACGTG 1410085 1.2312398 9.299751 170-179
TGGGC 1865375 1.2294285 31.665054 15-19
ACTTC 1072300 1.2237192 5.1881175 210-219
TATAC 887570 1.2185272 5.903774 110-119
TGATA 974960 1.2154197 6.9956145 180-189
GCTCA 1263505 1.2149755 8.844094 140-149
AAGTA 869515 1.2100459 15.565015 70-79
ACCAT 949155 1.2091717 9.115401 45-49
CCATG 1250265 1.2022442 9.622536 70-79
AGAGA 1024410 1.2012233 11.15736 35-39
TTGAG 1274580 1.199351 8.456693 200-209
GTCCA 1244100 1.1963158 11.089828 70-79
GCGTA 1365190 1.1920391 8.904747 210-219
GGAGT 1489920 1.181319 6.5198646 210-219
AAAGT 847485 1.1793883 18.720186 70-79
CTGAT 1133610 1.1747245 5.890638 180-189
ATATC 855215 1.1741077 5.1292143 70-79
TGTGC 1496815 1.1707932 5.9566975 220-229
CTTCT 1126090 1.1512065 5.049624 210-219
AGTTC 1110330 1.1506001 5.5485134 160-169
TATCA 832560 1.1430051 5.0775123 70-79
TAGAT 913050 1.1382405 7.662058 240-247
CCCAA 958750 1.1333772 8.892154 240-247
ACTGG 1289095 1.1255955 6.786215 160-169
CTGCG 1531350 1.1114879 6.20608 210-219
GTCTA 1071520 1.1103824 7.522972 180-189
GAACC 1022560 1.0976521 7.954781 70-79
TGCCC 1367940 1.0934287 9.118409 60-69
TGAAC 943880 1.0918789 5.0348787 190-199
AGAAA 699200 1.0862045 5.3647695 5
AAAAG 697070 1.0828955 11.1609 70-79
AGGTT 1150290 1.0823967 18.884195 10-14
ACCCA 901940 1.0662197 10.684342 240-247
AGACC 979365 1.051285 5.427496 240-247
GCGGT 1594390 1.050828 6.915707 130-139
TCTGT 1127835 1.0469658 5.1765003 210-219
GTGGT 1470520 1.0444552 7.392755 50-59
GCATA 900235 1.0413904 6.946679 210-219
TATGT 925250 1.0332686 6.944679 220-229
TGGTC 1315240 1.0287671 8.317669 50-59
CCAAT 806050 1.0268637 10.083014 240-247
TCTAC 897735 1.024504 6.998851 180-189
GGAAG 1153015 1.0205272 9.600821 30-34
ACAAT 658580 1.0093145 7.547079 100-109
CTCGG 1375865 0.9986334 6.782492 130-139
ACGTC 1032045 0.99240553 7.256676 70-79
ATAAC 642950 0.98536056 6.286531 80-89
TATAA 591670 0.9771906 6.605815 240-247
GTATG 1038140 0.9768663 5.234408 220-229
CCAGC 1089000 0.9717101 5.784728 80-89
TACTG 931955 0.9657557 5.107686 180-189
GCTTC 1120870 0.9655181 5.428552 150-159
CGGTC 1324915 0.96165276 6.8866644 130-139
TGTAA 766990 0.9561569 6.488638 230-239
TCCCC 1085625 0.95564455 5.474281 90-99
AGCTT 915900 0.9491183 5.694316 150-159
CGATG 1073050 0.93695194 22.512196 15-19
CGGAG 1248115 0.9182841 5.934965 210-219
CATCA 712755 0.9080111 5.1914377 230-239
ACTAA 592415 0.9079126 10.333086 240-247
ACCGT 936875 0.9008909 12.322082 45-49
TGAAG 835005 0.8771091 8.379059 35-39
AATCC 675780 0.86090684 9.977408 240-247
GACCG 1053090 0.8532595 12.495182 45-49
AGCTG 961910 0.8399082 6.379267 30-34
TGCAT 808365 0.83768326 5.8103313 210-219
GAGCT 954720 0.8336302 5.259854 140-149
CTACC 783600 0.82980895 6.0802655 230-239
TGCGT 1054685 0.82496357 6.7689137 210-219
AAAGG 701655 0.8227607 7.009213 10-14
CCTTT 802725 0.82062906 6.140304 50-59
ATCAA 517210 0.7926563 11.298324 70-79
AGCCA 729215 0.7827652 6.9426146 80-89
TGAGA 718320 0.7545405 5.660746 240-247
TAACC 573470 0.7305695 5.820169 80-89
TAGGT 767585 0.72228014 6.198149 10-14
CTAAT 522765 0.7176936 8.405344 240-247
AGGAT 683135 0.7175813 8.189786 10-14
CAATC 562740 0.7169001 9.916642 240-247
GGTCC 975370 0.7079452 9.043764 50-59
TGGAT 741105 0.6973631 6.6468325 10-14
ATACC 536345 0.68327427 5.662747 240-247
TCGAA 586315 0.6782483 11.391977 70-79
AAGGT 637135 0.6692617 6.9673095 10-14
ACGAT 563930 0.65235335 8.534973 10-14
CTTTG 699270 0.6491302 5.3430977 50-59
AATGG 616820 0.6479224 5.5700088 10-14
GTCGA 736650 0.64321864 7.9222856 70-79
CGAAC 568495 0.61024266 7.920983 70-79
AAAGC 460720 0.59494954 7.6793704 70-79
CATCC 561315 0.59441584 6.4818225 240-247
CGTCC 738105 0.58998585 5.7909145 70-79
GAAGC 595785 0.5807274 8.544248 30-34
TTGCC 641990 0.5530106 6.1393566 60-69
ATAGG 523860 0.550275 5.0831456 10-14
AAGCT 469505 0.54312265 13.301046 30-34
TCAAG 456605 0.5282 5.2689104 70-79
CGGTT 640440 0.50094545 9.965282 10-14
GGGAT 627505 0.4975325 6.1052094 10-14
TAAAG 356800 0.49653476 6.4638615 9
GACGA 496450 0.48390296 5.6142054 40-44
TCGAT 424660 0.4400618 6.505197 10-14
GCGAT 372310 0.3250889 5.195421 10-14