FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_18_SL254628.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_18_SL254628.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2971601
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3399195 8.203381 57.91601 110-119
CACAG 4454520 7.0928764 40.17677 110-119
TCCCT 4886010 6.576107 96.11325 30-34
CAGTA 3843375 6.475444 43.46601 60-69
ATACA 2756245 6.2863593 31.706753 110-119
GGAGA 4763400 6.1088223 91.995155 35-39
GGTGA 5217105 5.8273525 93.05204 45-49
AGTAA 2841385 5.8159547 42.79753 110-119
CCCTG 4987430 5.6933184 57.905457 30-34
GTAAT 3059590 5.4545064 37.263973 110-119
ACAGT 2928665 4.9343114 33.207977 110-119
AGACG 3441025 4.9172106 99.59381 40-44
GTGAC 3911280 4.868006 82.9572 45-49
GTAGT 3695730 4.8670735 24.877636 70-79
GGCCC 4987095 4.8284793 58.042923 60-69
GAGAC 3343215 4.7774396 129.2244 40-44
CCCCA 3228810 4.7154193 99.66047 60-69
ACCAG 2778485 4.4241467 97.43535 45-49
GTGTC 4039760 4.379143 25.312223 120-129
AATAC 1916780 4.371733 31.816322 110-119
ACTCC 2799395 4.3259096 96.784744 30-34
GCACA 2670705 4.2525296 33.1938 110-119
CGTGT 3902575 4.230433 24.13211 120-129
TGACC 3020715 4.1892133 150.65335 45-49
GACCA 2560145 4.0764866 108.7001 45-49
CCCTT 2959730 3.9835165 59.4912 20-24
TAGTA 2232105 3.9793015 24.512178 80-89
CTCAG 2857845 3.9633405 30.393942 140-149
CACTC 2559335 3.9549446 81.51803 25-29
CCACT 2541510 3.9273992 27.94729 240-247
CCCAG 2995175 3.9256315 82.671715 60-69
TGGTG 4011265 3.9023337 26.438793 25-29
AGTAG 2560230 3.8711975 28.610498 70-79
TGAGG 3437835 3.8399608 70.053955 35-39
CAGGG 3548075 3.745408 34.89392 50-59
CTCCC 2923320 3.7183948 59.729916 30-34
GACGG 3486155 3.6800442 112.19471 40-44
AGGAG 2855770 3.6623821 92.01416 35-39
CTGAG 2913045 3.625596 98.08038 35-39
ACGGT 2880185 3.584698 93.91615 40-44
CTTGG 3282840 3.5586336 34.468773 20-24
ATGGT 2688260 3.5402906 24.882463 190-199
GCCCC 3263860 3.521152 73.5008 60-69
TCTTG 2743190 3.506027 19.165085 170-179
TGTCT 2722165 3.4791555 20.70114 180-189
AGATA 1676675 3.4319408 18.53716 150-159
AGGCT 2724260 3.390633 41.962887 30-34
GAGGA 2643185 3.3897526 128.95207 35-39
ATGCA 1993470 3.3586633 81.716034 25-29
CCAGG 2855280 3.3585017 40.223675 50-59
GGGTT 3396430 3.3041954 44.845154 50-59
TGTCC 2735525 3.3041878 19.583714 120-129
TTCCC 2453650 3.3023806 57.158985 50-59
CCTGG 3194795 3.272963 32.24815 50-59
CTTCA 1992225 3.257519 16.34889 200-209
GCCCT 2826175 3.2261734 31.046642 20-24
TCAGG 2562215 3.1889508 20.139875 140-149
CTCTC 2363435 3.1809597 14.125183 100-109
TGCAC 2237005 3.102342 104.87266 25-29
CCGTG 3000590 3.0740066 24.118492 120-129
GATGG 2750470 3.0721943 30.654709 15-19
ACAGA 1582660 3.0615628 14.270974 140-149
GGATG 2735535 3.0555122 58.553017 20-24
CCAGT 2201905 3.0536642 42.371418 60-69
GGTTC 2813800 3.0501893 50.45722 50-59
TCGCA 2195745 3.0451214 24.838707 100-109
TACAC 1583745 2.973258 23.057022 110-119
CGCAC 2268145 2.9727483 25.383123 100-109
TCTCA 1807935 2.9561834 20.854488 130-139
GACTC 2107615 2.9229002 21.260828 190-199
TGGCC 2839195 2.9086628 39.763737 60-69
TGGAG 2596275 2.899963 29.182035 25-29
AGGGT 2592520 2.8957686 52.49025 50-59
CCTGA 2076375 2.8795757 68.19585 30-34
TCTCT 2018200 2.874181 15.313863 180-189
GTTCC 2372310 2.8654675 57.379272 50-59
GCACT 2048580 2.841029 74.16955 25-29
TTCTT 1873515 2.8232052 21.257206 170-179
CGGAT 2259725 2.8124692 66.12321 20-24
CAGAT 1658125 2.7936633 15.432785 150-159
GGTTG 2850475 2.7730663 61.188007 15-19
GCCGT 2700335 2.766405 22.81239 120-129
CGGTG 2975085 2.735313 68.108765 40-44
TCAAA 1194935 2.7253716 56.39946 70-79
ACCAC 1531035 2.716421 15.184915 230-239
TCACA 1444125 2.711141 12.298985 130-139
AGTCA 1606130 2.7060606 30.396727 70-79
AAAAA 845680 2.690421 11.414575 220-229
AATAA 958790 2.6566782 12.723414 110-119
AGGGA 2063545 2.6463933 9.76741 150-159
CGGCC 2692885 2.6072373 11.196662 120-129
GGCTG 2816840 2.5898216 23.121166 30-34
GTTGG 2653025 2.5809784 47.59924 15-19
GATGC 2034670 2.5323646 77.93508 25-29
CTGGC 2451585 2.511569 35.65801 60-69
AGATG 1643960 2.4857507 16.773623 180-189
TGTTC 1910980 2.4423928 12.751201 140-149
GAGAT 1574785 2.3811545 16.174671 180-189
ACTCT 1441210 2.3565457 12.961594 190-199
GTCTC 1940025 2.3433187 12.517791 180-189
CAGAG 1634640 2.3358934 9.235674 140-149
GTTCT 1824520 2.3318899 18.496008 160-169
TCATT 1342015 2.3218887 16.367586 150-159
CATTT 1332615 2.3056252 17.470655 150-159
TTCAT 1330470 2.301914 17.324642 140-149
TTGTC 1793165 2.2918153 14.98865 170-179
CTGGA 1840475 2.290668 14.573442 180-189
ACAGC 1433230 2.282114 11.996554 110-119
TCCAT 1395385 2.2816164 11.23342 70-79
TCCTC 1671330 2.2494519 14.811833 130-139
GGCGG 2881705 2.247146 37.80245 20-24
GCGGA 2108020 2.2252617 79.6167 20-24
TGTAG 1688525 2.2236943 8.900017 210-219
CACTA 1181465 2.218034 14.809568 240-247
TAGTC 1510730 2.2168941 25.904303 70-79
ATATA 918305 2.2161734 10.72968 110-119
GTGAA 1443575 2.1827586 16.521303 190-199
ACTAC 1158010 2.1740005 12.670404 230-239
TTCAC 1316085 2.1519518 16.98227 200-209
TTGGT 1874190 2.149723 37.985836 25-29
TGAAT 1185890 2.114154 14.530973 190-199
CTACT 1288315 2.1065447 8.698712 230-239
AATAT 869380 2.0981011 12.261002 110-119
GAATC 1244140 2.096168 15.064963 190-199
ATTTG 1348250 2.0934565 15.1151905 150-159
GTCAA 1238590 2.0868173 40.794006 70-79
GAGTC 1675355 2.0851586 12.6137295 210-219
TTGGA 1581075 2.0821886 16.686235 170-179
CTCTG 1719000 2.0763466 14.913242 180-189
TCTGC 1718335 2.0755436 10.767464 210-219
CAGCC 1582880 2.0746045 10.885213 120-129
GATAC 1223730 2.0617807 13.593247 150-159
TCTCG 1705220 2.0597022 16.903954 100-109
TTGGG 2108560 2.051299 74.65833 15-19
GATAT 1148360 2.0472472 9.90418 180-189
CTCGC 1778350 2.030046 17.087004 100-109
GTTCA 1381370 2.027067 15.488726 140-149
ATATG 1136760 2.0265672 9.930163 180-189
TCGGC 1969595 2.0177858 8.590583 190-199
CCTTC 1499055 2.0175862 13.161432 200-209
AATAG 963265 1.9716812 8.16139 240-247
ATAGA 959555 1.9640872 9.927707 240-247
AATCG 1162115 1.9579693 13.569016 190-199
CACGG 1657840 1.950022 7.373325 200-209
TACTA 981430 1.9495844 11.176563 230-239
TACAG 1153080 1.9427471 12.093982 160-169
ACACA 898325 1.9363306 13.561619 110-119
TACCA 1030325 1.9342899 12.339786 230-239
GCTGT 1758270 1.9059834 9.806496 140-149
CCTTG 1570950 1.8975201 38.97217 20-24
TTGCA 1292290 1.8963481 13.139123 150-159
TCCCA 1223925 1.8913331 11.598525 240-247
GGTCA 1519575 1.8912735 10.0048 130-139
GAACT 1122295 1.8908795 10.853408 160-169
GTTGT 1644345 1.8860875 7.753597 210-219
GTCAC 1359480 1.8853654 9.46036 130-139
GTCCT 1547080 1.8686881 12.726849 130-139
GCTGA 1492520 1.8576008 35.873302 30-34
AGTAT 1033280 1.8420874 11.695489 220-229
TGGAA 1216495 1.8394021 8.966084 170-179
AGTCT 1251425 1.8363817 13.150076 210-219
TTTGC 1434040 1.8328236 11.628581 150-159
ATTGT 1179400 1.8312795 8.437661 170-179
CATAG 1082645 1.8240759 8.188439 210-219
TGGGG 2204750 1.8191837 44.621494 15-19
TCAGC 1309760 1.8164123 11.967475 140-149
CAGGC 1541490 1.8131659 13.851931 140-149
CTGTT 1414925 1.808393 12.463892 140-149
GTCTG 1623165 1.7595282 8.741507 210-219
ATAGT 983940 1.7541261 7.1432796 220-229
TTGTA 1116275 1.7332639 10.186356 210-219
CTCCA 1119055 1.7292773 8.238314 240-247
ATGTA 966865 1.7236856 9.349958 70-79
CAGCT 1238055 1.7169697 9.378704 140-149
ATCTC 1046655 1.7114025 6.9876294 100-109
AGAAC 876325 1.6951991 10.755542 150-159
TTCAG 1147210 1.6834531 10.0177765 150-159
GGGGC 2158150 1.6829196 41.137356 15-19
CACCA 944565 1.6758834 10.721754 230-239
GCAGA 1168735 1.6701173 13.410404 150-159
TCTGG 1539195 1.6685038 11.825882 180-189
TGACT 1135175 1.6657927 12.477227 190-199
ACGGC 1416150 1.6657356 12.529568 120-129
TGCAG 1317905 1.6402738 12.453071 150-159
ATAAT 672655 1.6233387 5.515929 100-109
CCCAC 1110245 1.6214242 10.796633 240-247
GAGAA 928160 1.6113425 10.534293 160-169
GACGT 1294180 1.6107453 10.127703 170-179
GGGCG 2060220 1.6065542 49.13725 20-24
GGAGG 1693435 1.6042957 25.280046 30-34
GTGGA 1434115 1.6018645 35.92533 25-29
AACCA 741130 1.5974983 12.332164 70-79
GGACG 1511410 1.5954701 8.007169 170-179
CACTG 1150330 1.5953102 7.1560416 230-239
CCAGA 997530 1.5883543 21.560827 60-69
ATAAA 568605 1.575528 6.3841295 8
CAAAG 813125 1.5729425 29.469812 70-79
AACTG 933570 1.5729092 10.557675 160-169
ACTGA 927995 1.5635165 7.4254026 180-189
TGGTT 1361970 1.5621995 16.388697 10-14
AGCTC 1125430 1.5607783 9.956651 140-149
CAAAA 589420 1.5434941 19.496647 70-79
CGACT 1103365 1.5301778 9.726088 190-199
CTCTT 1073145 1.528299 8.852813 200-209
ACACG 948075 1.5096078 9.602828 110-119
TCAGA 892895 1.5043789 9.14634 130-139
TCGAC 1080930 1.4990643 10.213968 190-199
TCCAC 964815 1.49093 10.738076 240-247
ACGGA 1043205 1.4907354 9.798789 200-209
GTCCC 1300475 1.4845357 15.3273535 50-59
ATCGG 1185620 1.4756309 9.417258 190-199
GCTCT 1219265 1.4727267 9.270076 130-139
CCTCG 1288485 1.4708488 8.958552 130-139
CTGGT 1350740 1.4642166 6.990645 160-169
ATGGG 1298535 1.4504255 50.01957 15-19
GAGGG 1527200 1.446811 9.533037 200-209
TGGAC 1161220 1.4452626 10.972908 170-179
CCATC 933885 1.4431338 11.395758 240-247
AAATA 518285 1.4360981 5.600654 6
TAGTT 922855 1.4329365 8.732192 220-229
AGCCG 1214350 1.42837 10.4885 120-129
GGCCG 1642060 1.426794 11.888672 120-129
AATTG 797610 1.421945 9.389498 170-179
CCACC 973070 1.4210911 8.057917 230-239
CTCGA 1019020 1.4132059 9.303475 190-199
CTTGA 954830 1.4011484 9.438464 200-209
CGTAG 1113955 1.3864362 8.066945 210-219
ACTAT 689950 1.3705672 6.772961 230-239
TATGG 1040700 1.3705446 7.169292 180-189
GGGAG 1446365 1.3702309 7.0787582 150-159
ATCCA 728345 1.367365 17.09211 240-247
CAATA 596905 1.3614028 7.1687875 100-109
GAATT 759495 1.3539951 10.6028 170-179
GTGCT 1229195 1.3324605 7.3456326 220-229
ACCAT 707620 1.3284568 9.791921 45-49
GGCTC 1290355 1.3219266 8.297205 130-139
AAGTA 643960 1.3181044 17.826046 70-79
TATAC 661210 1.3134758 6.6413817 110-119
CATGT 889890 1.3058535 9.019985 70-79
TTCCA 797410 1.3038579 7.339494 230-239
ACTGT 887095 1.3017521 5.746657 230-239
ACGTG 1041715 1.2965257 9.732239 170-179
AAAGT 631820 1.2932552 22.38397 70-79
TGATA 721500 1.2862593 7.144847 180-189
ACTCG 925065 1.2829064 9.602405 190-199
AGAGT 844930 1.2775769 5.0007453 200-209
CAGAC 797885 1.270462 10.778939 60-69
TGTGC 1170555 1.2688941 6.1124573 220-229
ACTTC 774410 1.2662503 5.4967403 220-229
GAGGC 1196675 1.2632304 22.30006 30-34
GCTCA 905615 1.2559326 9.667849 140-149
CTGAT 853090 1.251852 6.322635 180-189
GTGTT 1083395 1.2426698 7.3744216 160-169
CCATG 895440 1.2418215 9.055205 70-79
GGAAT 819500 1.2391255 8.583336 170-179
TCACG 892565 1.2378345 8.365862 200-209
TATCA 621765 1.2351195 5.5522175 70-79
TTGAG 931575 1.226833 8.595628 200-209
CCTCA 786280 1.2150396 5.8685675 130-139
GGAGT 1079340 1.2055911 6.900821 210-219
AGTTC 813590 1.1938884 5.9178147 160-169
AGAGA 687505 1.1935507 12.318786 35-39
GAACC 747355 1.1900039 9.092622 70-79
GGTGG 1439075 1.1874095 36.426003 25-29
CTGAA 704105 1.1862993 13.165618 35-39
GGGCC 1358715 1.1805942 27.76586 15-19
GTCCA 848980 1.1773895 10.373415 70-79
GCGTA 941525 1.1718286 8.9539795 210-219
GTCTA 781930 1.1474293 7.5123773 180-189
TGGGC 1246310 1.1458658 29.8351 15-19
ACTGG 919095 1.1439121 6.951226 160-169
ATCAT 575825 1.1438609 5.3892756 90-99
AGATC 677540 1.1415417 5.9073625 130-139
GCATA 676635 1.1400168 7.9249735 210-219
CTGCG 1108120 1.1352328 6.332991 210-219
TGCCC 973230 1.1109747 8.691549 60-69
TAGAT 619015 1.1035534 7.9980354 240-247
ACAAT 483280 1.1022503 8.514274 100-109
GAAGA 627785 1.0898732 17.353222 35-39
TCTAC 664455 1.0864611 6.9590864 180-189
CCAAT 578025 1.0851604 11.324315 240-247
TATGT 697340 1.0827746 7.006273 220-229
AGAGC 757600 1.0826072 6.4340487 140-149
CCCAA 606795 1.0765989 9.800911 240-247
AGGTT 812195 1.0696162 18.768557 10-14
GCGGT 1159420 1.0659785 7.0112815 130-139
AAAAG 453415 1.0655786 12.865604 70-79
CGGCT 1039420 1.0648519 7.269639 130-139
CTCGG 1038765 1.0641809 7.3899703 130-139
ATAAC 462230 1.0542401 7.135346 80-89
TATAA 428130 1.0332191 7.3302574 240-247
TCTGT 792280 1.0126004 5.7081647 210-219
AAGAG 583225 1.0125142 12.278914 35-39
TGGTC 930980 1.0091923 7.530403 50-59
TCCAG 725690 1.0064075 5.434788 240-247
TACTG 680850 0.9991014 5.164431 180-189
ATATC 502555 0.99831194 5.539953 70-79
TGTAA 553410 0.9865957 6.879792 230-239
CGGTC 961410 0.9849332 7.2516713 130-139
GTGGT 1010975 0.9835206 6.65052 50-59
GCCAC 744655 0.97598344 5.0985727 80-89
GTCAG 778175 0.968522 5.4171405 130-139
ACTAA 422955 0.96466297 11.187841 240-247
ACCCA 543160 0.9636953 11.429467 240-247
GCTTC 796240 0.9617629 5.283525 150-159
AGCTT 653510 0.9589817 5.5923963 150-159
GAGTT 727500 0.95807755 5.301824 160-169
CGGAG 901750 0.95190257 6.4035053 210-219
CATCA 506565 0.9510044 5.5542135 230-239
TAGAC 558275 0.9406001 5.0359507 110-119
CTTCC 687690 0.92556566 5.255449 230-239
CTACC 596890 0.9223751 6.842834 230-239
CGATG 732560 0.9117493 20.947306 15-19
TGAAG 588795 0.8902879 9.452222 35-39
ACCGT 639665 0.88710564 12.156835 45-49
CCAGC 671695 0.8803583 5.817785 80-89
AATCC 467880 0.8783787 10.740639 240-247
TGCAT 597165 0.8762993 6.51613 210-219
ACGTC 625965 0.868106 6.913841 70-79
GAGCT 692710 0.8621517 5.43689 140-149
TGCGT 788325 0.8545527 6.8567924 210-219
TGAAC 504655 0.8502594 5.133414 190-199
AGCCA 527555 0.84001917 7.8958683 80-89
ATCAA 365255 0.8330625 12.201807 70-79
AGCTG 667240 0.83045155 6.557066 30-34
AAAGA 347010 0.8155143 5.13771 70-79
AGACC 506270 0.8061273 5.565962 240-247
TAACC 423845 0.7957092 6.525844 80-89
GGAAG 605210 0.77615154 8.711415 30-34
CTAAT 388835 0.77241033 9.012862 240-247
CCTTT 536950 0.76468706 5.7627535 50-59
AAAGG 433595 0.7527474 7.3036046 10-14
CAATC 400230 0.7513754 10.61544 240-247
TGAGA 486935 0.73627037 5.935554 240-247
TCGAA 430810 0.7258429 12.6865225 70-79
ATACC 383550 0.720061 6.236995 240-247
TAGGT 542500 0.7144427 5.9722714 10-14
AGGAT 464800 0.7028011 7.836858 10-14
TGGAT 518860 0.6833101 6.8545833 10-14
GTCGA 544135 0.67723423 9.031137 70-79
AATGG 442660 0.66932434 5.3519 10-14
GACCG 564925 0.6644888 12.508847 45-49
ACGAT 389280 0.6558717 8.114103 10-14
CGAAC 411200 0.6547485 9.055461 70-79
AAGGT 432920 0.654597 6.8200974 10-14
GGTCC 637960 0.65356916 8.392982 50-59
CGTCC 569525 0.6501319 5.4590898 70-79
CATCC 419315 0.6479682 7.590413 240-247
AAAGC 324365 0.62746495 8.706847 70-79
ATAGG 374010 0.5655221 5.1943264 10-14
GAAGC 381720 0.5454762 8.222273 30-34
TTGCC 432965 0.5229701 5.949831 60-69
TCAAG 309905 0.5221381 5.3430185 70-79
AAGCT 305310 0.5143963 13.391906 30-34
CGGTT 462320 0.5011598 10.160582 10-14
GACGA 350345 0.50064147 6.2937565 40-44
TAAAG 242445 0.49625415 5.3504014 9
GGGAT 420780 0.46999887 5.5428653 10-14
AAGCA 238185 0.46075487 5.394367 70-79
TCGAT 283370 0.41582635 6.1115174 10-14
GCGAT 244480 0.30428147 5.009231 10-14