FastQCFastQC Report
Fri 7 Jul 2017
HNCHNBCXY_s1_1_illumina12index_2_SL254629.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_2_SL254629.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2303631
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2592310 8.029774 56.34945 110-119
CACAG 3384910 6.9400487 39.85103 110-119
TCCCT 3696595 6.48239 93.41867 30-34
CAGTA 2929930 6.350129 42.012684 60-69
ATACA 2106860 6.173651 30.597109 110-119
GGAGA 3627555 5.94551 89.852905 35-39
GGTGA 3978130 5.7016816 90.76001 45-49
AGTAA 2171540 5.689239 42.484352 110-119
CCCTG 3787300 5.617369 56.254288 30-34
GTAAT 2344065 5.370378 36.84179 110-119
AGACG 2643855 4.8465524 96.228355 40-44
ACAGT 2234165 4.8421755 33.517326 110-119
GTAGT 2850030 4.8295145 24.665218 70-79
GGCCC 3840645 4.818117 56.704105 60-69
GTGAC 2975215 4.769386 80.821304 45-49
CCCCA 2497715 4.7382436 98.57936 60-69
GAGAC 2553310 4.680571 125.443794 40-44
ACTCC 2190120 4.3918986 95.27524 30-34
ACCAG 2138770 4.3850994 94.59582 45-49
GTGTC 3111515 4.361791 24.580303 120-129
AATAC 1463885 4.2895665 30.899666 110-119
GCACA 2065240 4.2343416 33.216026 110-119
CGTGT 2990650 4.19236 23.449198 120-129
TGACC 2308125 4.138315 147.68904 45-49
CACTC 2001855 4.014366 81.30286 25-29
CCCTT 2282345 4.002345 60.735218 20-24
GACCA 1949255 3.9965389 105.3437 45-49
TAGTA 1734250 3.9732594 25.82174 80-89
CTCAG 2192060 3.9302185 29.894829 140-149
CCACT 1956010 3.9224324 27.72028 240-247
TGGTG 3126880 3.9190793 26.923378 25-29
CCCAG 2277140 3.862283 81.45301 60-69
AGTAG 1967275 3.8121521 27.416548 70-79
TGAGG 2655130 3.8054824 67.718925 35-39
CTCCC 2242905 3.7207823 57.422337 30-34
CAGGG 2655985 3.6011376 33.32522 50-59
GACGG 2655565 3.600568 108.63446 40-44
CTGAG 2236150 3.5846362 95.42732 35-39
AGGAG 2181735 3.5758324 89.423904 35-39
CTTGG 2546715 3.5700421 35.609234 20-24
AAAAA 876325 3.5496361 18.557562 230-239
TGTCT 2121645 3.5163794 20.808342 180-189
ATGGT 2073755 3.5140786 25.281563 190-199
GCCCC 2489980 3.4937284 72.65344 60-69
ACGGT 2178495 3.492213 91.71295 40-44
TCTTG 2085370 3.4562578 19.275034 170-179
AGATA 1296085 3.395626 17.88767 150-159
AGGCT 2091795 3.3532295 41.318962 30-34
GAGGA 2043575 3.34939 124.11339 35-39
CTTCA 1567385 3.3225381 16.646502 200-209
ATGCA 1522145 3.2989924 79.327034 25-29
TGTCC 2101015 3.2941415 18.969467 120-129
CCAGG 2142950 3.2497225 39.01106 45-49
GCCCT 2190940 3.2496285 31.934967 20-24
TTCCC 1850140 3.244426 55.73485 50-59
CCTGG 2440130 3.235907 31.735176 50-59
GGGTT 2574040 3.226177 43.111958 50-59
TCAGG 1969055 3.1564724 19.884253 140-149
CTCTC 1798530 3.153922 13.772946 100-109
GATGG 2183085 3.1289213 31.196665 15-19
CCGTG 2323455 3.0811822 23.512026 120-129
GGATG 2148065 3.0787282 57.692963 20-24
TGCAC 1696675 3.0420258 103.55075 25-29
GGTTC 2155890 3.0221746 48.67747 50-59
CCAGT 1682835 3.0172117 41.017372 60-69
ACAGA 1212930 3.0061603 14.228148 140-149
TCGCA 1672630 2.998915 24.113865 100-109
CGCAC 1735785 2.9440846 24.800163 100-109
TACAC 1214085 2.9430287 21.99435 110-119
GACTC 1616915 2.8990216 20.604221 190-199
TGGAG 2009925 2.8807383 29.960485 25-29
TCTCA 1355270 2.8728974 20.191378 130-139
TGGCC 2157200 2.8607078 38.79095 60-69
TCTCT 1529880 2.8359628 15.53384 180-189
CAGAT 1298285 2.8138137 15.147241 150-159
GCACT 1569240 2.8135433 73.711464 25-29
ACCAC 1223060 2.8046796 15.517287 230-239
CCTGA 1564285 2.8046594 66.092125 30-34
GTTCC 1782435 2.794646 55.57473 50-59
TTCTT 1424480 2.7913187 21.55356 170-179
AGGGT 1938880 2.778913 50.214447 50-59
CGGAT 1731115 2.775045 65.44993 20-24
GCCGT 2084620 2.7644582 22.508663 120-129
GGTTG 2188115 2.742477 60.049812 15-19
AGTCA 1252985 2.7156336 29.940142 70-79
CGGTG 2272135 2.693997 66.72872 40-44
TCAAA 914425 2.6795049 55.08365 70-79
TCACA 1086630 2.634069 11.836135 140-149
AGGGA 1588010 2.6027255 9.242982 150-159
CGGCC 2060160 2.5844858 11.176165 120-129
AATAA 726820 2.574509 12.349637 110-119
GGCTG 2165630 2.5677173 23.291227 30-34
GTTGG 2023010 2.5355422 46.595264 15-19
GATGC 1556875 2.4957318 76.04396 25-29
AGATG 1280650 2.481622 16.331417 180-189
CTGGC 1850625 2.454152 34.751793 60-69
ACTCT 1134360 2.404613 12.924634 190-199
TGTTC 1445080 2.3950517 12.769499 140-149
GAGAT 1231345 2.3860798 16.099964 180-189
TCCTC 1329440 2.3313208 14.758568 130-139
GTTCT 1385055 2.2955673 17.935371 160-169
GTCTC 1456675 2.283893 12.574848 180-189
TCCAT 1070285 2.2687871 12.1635895 70-79
TGTAG 1333800 2.2601888 8.643701 210-219
TTGTC 1363520 2.2598755 14.674977 170-179
CTGGA 1407715 2.256622 14.816643 180-189
CAGAG 1230925 2.2564561 9.16084 140-149
TCATT 1003305 2.2482119 16.152264 150-159
CATTT 1002675 2.2468 17.359468 150-159
GGCGG 2220970 2.2272842 36.8667 20-24
CACTA 913495 2.2143772 14.175842 240-247
TTCAT 984670 2.2064543 16.49081 140-149
ACAGC 1075450 2.204985 11.43252 120-129
TTCAC 1037235 2.1987278 16.887663 200-209
TAGTC 1151940 2.1832528 25.413475 70-79
GCGGA 1607530 2.1795819 78.15733 20-24
TTGGT 1460135 2.1636941 39.300373 25-29
TCTGC 1366030 2.1417727 10.971574 210-219
ATATA 685225 2.1225092 10.15792 110-119
CTCTG 1343620 2.1066365 14.941942 180-189
ACTAC 866830 2.1012578 12.161982 230-239
TTGGA 1233795 2.0907257 16.578133 170-179
GTGAA 1078320 2.08955 16.583052 190-199
GATAC 955520 2.0709283 12.938607 150-159
TCTCG 1316850 2.0646644 16.394236 100-109
AATAT 666050 2.063114 12.113986 110-119
GATAT 892855 2.0455785 9.420766 180-189
GAGTC 1275210 2.0442116 12.440525 210-219
ATTTG 1019820 2.0431845 15.059891 150-159
TTGGG 1630035 2.0430067 73.43689 15-19
CTACT 962830 2.0410044 8.232259 230-239
GTTCA 1074070 2.035667 15.307877 140-149
GTCAA 938210 2.0334117 40.015896 70-79
CTCGC 1366870 2.027358 16.588993 100-109
TGAAT 874145 2.002713 14.078619 190-199
ATGTA 873035 2.0001698 10.441601 70-79
ATATG 872260 1.9983943 9.601874 180-189
GAATC 920330 1.99466 14.271794 190-199
CCTTC 1131595 1.9843774 13.661931 200-209
TCGGC 1489495 1.9752502 8.120726 190-199
CAGCC 1160495 1.9683287 10.621706 120-129
CCTTG 1243640 1.9498795 40.3331 20-24
AATAG 740800 1.9408293 8.369498 240-247
CACGG 1273475 1.9311885 7.201692 200-209
GAACT 885505 1.9191828 11.643489 160-169
TCCCA 956960 1.9190143 11.917399 240-247
ACACA 692160 1.9186823 12.788654 110-119
GTCAC 1064060 1.907789 9.224075 140-149
ATAGA 728165 1.9077265 9.980307 240-247
TACAG 875945 1.8984629 11.638937 160-169
AATCG 875400 1.8972819 12.888339 190-199
TACCA 782050 1.895745 12.067077 230-239
GCTGT 1340110 1.8785961 9.673153 140-149
TCAGC 1042850 1.869761 11.889513 140-149
TTGCA 983070 1.8631963 13.230946 150-159
GTTGT 1253320 1.8572263 7.1150312 210-219
GTCCT 1184000 1.856371 12.610791 130-139
TGGAA 951365 1.8435389 8.812959 170-179
GGTCA 1149090 1.8420361 9.571329 130-139
TACTA 718470 1.841043 10.526343 230-239
TTTGC 1109370 1.8386518 11.580611 150-159
GTCTG 1311380 1.8383217 8.737526 210-219
GCTGA 1137790 1.8239218 35.932266 30-34
CACCA 795340 1.8238468 11.172425 230-239
ATTGT 907290 1.8177332 8.482632 170-179
CAGGC 1195775 1.8133588 13.860358 140-149
AGTAT 791335 1.8129908 11.6774845 220-229
TGGGG 1698705 1.8007805 44.068935 15-19
CATAG 823665 1.7851548 7.86093 210-219
AGTCT 938315 1.778373 12.575758 210-219
CTGTT 1067830 1.769804 12.382043 140-149
ATCTC 832955 1.7656956 7.0905185 100-109
TTCAG 925830 1.7547103 9.95054 150-159
CTCCA 872930 1.750507 8.727168 240-247
CAGCT 966540 1.7329422 9.269252 150-159
TTGTA 861055 1.7251025 9.459504 210-219
ATAGT 734335 1.6824007 7.05533 220-229
CCCAC 877490 1.6646259 10.674692 240-247
AGAAC 670985 1.6629885 10.549574 160-169
GTGGA 1158175 1.659962 36.774452 25-29
GGGGC 1654460 1.6591635 40.664654 15-19
ACGGC 1093765 1.6586635 12.464086 120-129
TCTGG 1182430 1.6575569 12.149095 180-189
GGAGG 1365560 1.6554067 25.65151 30-34
TGACT 867245 1.6436752 12.079053 190-199
CCAGA 801530 1.6433693 21.809055 60-69
GCAGA 895835 1.6421897 12.945862 150-159
GACGT 1020125 1.6353004 10.007339 170-179
CAAAG 659220 1.6338296 28.591318 70-79
TGCAG 995490 1.5958093 12.461272 150-159
GGGCG 1590840 1.5953627 47.959198 20-24
AACCA 573855 1.5907383 12.217434 70-79
GAGAA 717700 1.590374 10.541399 160-169
TGGTT 1070170 1.5858263 16.920979 10-14
GGACG 1167685 1.5832145 7.8897853 170-179
CACTG 880020 1.5778176 6.7556157 230-239
CTCTT 849550 1.5748245 9.167335 200-209
AACTG 726300 1.5741327 10.383025 160-169
CAAAA 462450 1.5496099 19.78574 70-79
AGCTC 862580 1.5465488 9.6573925 140-149
ATAAA 436105 1.5447513 6.386777 110-119
ATAAT 496900 1.5391656 5.23543 110-119
ACACG 750650 1.5390505 9.521655 120-129
CGACT 852535 1.5285388 9.397854 190-199
GTCCC 1029295 1.5266627 16.07456 50-59
CTGGT 1078320 1.511613 7.082469 160-169
ACTGA 696900 1.510413 7.535774 180-189
TCGAC 840250 1.5065126 9.963333 190-199
CCACC 793565 1.5054177 8.28675 230-239
TCAGA 694305 1.504789 8.87128 130-139
ATGGG 1041615 1.4929016 50.549515 15-19
ACGGA 810030 1.4848973 9.337972 200-209
GCTCT 939460 1.4729617 9.049816 130-139
GAGGG 1208250 1.464707 9.016255 200-209
ATCGG 911330 1.4608976 8.995026 190-199
CCTCG 982675 1.4575154 9.0273485 130-139
TGGAC 908620 1.4565533 10.94435 170-179
TCCAC 724625 1.4531075 10.417624 240-247
GGCCG 1279925 1.4356136 11.889803 120-129
CTTGA 755865 1.4325784 9.343637 200-209
AATTG 622325 1.4257799 9.485142 170-179
CGTAG 883405 1.4161329 8.033561 210-219
TAGTT 703790 1.4100261 8.772576 220-229
CTCGA 773870 1.3874977 8.925181 190-199
GGGAG 1143605 1.3863407 6.652446 150-159
CCATC 690030 1.3837332 11.419641 240-247
AGCCG 899500 1.364066 10.194976 120-129
CAGAC 660500 1.3542169 11.371243 60-69
CATGT 712280 1.3499725 9.945787 70-79
GAATT 584110 1.3382274 10.459523 170-179
ACTAT 521985 1.3375602 7.034652 230-239
TATGG 787415 1.334313 6.956438 180-189
GGCTC 991355 1.3146565 8.094332 130-139
TATAC 510510 1.308156 6.4609427 110-119
GTGCT 931640 1.3059937 7.322196 220-229
CAATA 444415 1.3022523 7.281519 100-109
ACCAT 536705 1.3010112 9.902801 45-49
GAGGC 956290 1.2965931 22.27823 30-34
ACGTG 803395 1.2878737 9.466557 170-179
ACTCG 715800 1.283382 9.205374 190-199
CCATG 714695 1.2814008 9.99336 70-79
ACTGT 674735 1.2788141 5.4458313 230-239
GGAAT 659190 1.2773671 8.293297 170-179
CCTCA 635395 1.2741723 5.7089734 130-139
GCTCA 709910 1.2728217 9.210569 140-149
TGATA 555170 1.2719241 7.2245398 180-189
TTCCA 599320 1.2704368 7.341853 230-239
AAAGT 483450 1.2665954 21.267277 70-79
ACTTC 594135 1.2594455 5.4110665 220-229
TGTGC 896530 1.2567757 6.4989424 220-229
AAGTA 479110 1.2552251 16.940908 70-79
GTGTT 843970 1.2506329 7.227572 160-169
GGTGG 1168040 1.2382277 36.676098 25-29
ATCCA 509695 1.235537 16.510044 240-247
GTCCA 682120 1.222996 11.340463 70-79
TATCA 476875 1.221968 5.4768195 70-79
GGAGT 849010 1.2168493 6.774094 210-219
GAACC 592200 1.2141821 9.135202 70-79
CTGAA 560160 1.2140523 12.674766 35-39
CTGAT 639895 1.2127825 6.2501926 180-189
TTGAG 710030 1.2031803 8.432728 200-209
GCGTA 748150 1.1993136 8.939665 210-219
AGAGA 540425 1.1975447 12.020522 35-39
GGGCC 1065575 1.1951903 28.703585 15-19
AGATC 544015 1.1790608 5.91026 130-139
TGGGC 990565 1.1744809 30.672678 15-19
AGTTC 618465 1.1721665 5.620338 160-169
ACTGG 721320 1.1563041 7.048776 160-169
GAAGA 519750 1.1517304 16.774118 35-39
CTGCG 865635 1.1479367 6.27543 210-219
TGCCC 773525 1.1473017 9.21017 60-69
GCATA 522870 1.1332326 7.817293 210-219
CCCAA 491680 1.1275039 9.8018055 240-247
ATCAT 439900 1.1272215 5.023013 90-99
AGAGC 613445 1.1245297 6.621948 140-149
GTCTA 588685 1.1157249 7.4621186 180-189
ACAAT 379205 1.11117 8.532437 100-109
TCACG 615910 1.1042858 8.345405 200-209
AGGTT 643875 1.0910776 18.549757 10-14
TAGAT 475160 1.088617 7.793196 240-247
AAGAG 490825 1.0876346 11.821885 35-39
CGATG 678300 1.0873415 21.23014 15-19
GCGGT 916180 1.086285 6.794032 130-139
TATGT 539085 1.0800436 7.1451473 220-229
AAAAG 356555 1.0682281 12.783492 70-79
TCTAC 501675 1.0634493 7.052638 180-189
CGGCT 796415 1.0561424 7.328255 130-139
TCTGT 628515 1.0416905 5.3509026 210-219
CTCGG 785260 1.0413496 7.3433256 130-139
ATATC 405855 1.039983 5.4227166 70-79
TGGTC 737095 1.0332762 8.127365 50-59
CCAAT 423255 1.0260004 10.850035 240-247
ATAAC 346685 1.0158778 6.7988653 80-89
GTGGT 807465 1.0120375 7.1088767 50-59
TGTAA 432380 0.9906056 6.547367 230-239
TATAA 319695 0.99026674 6.8547554 240-247
TACTG 521055 0.98754686 5.234673 180-189
TCCAG 545625 0.9782695 5.548041 240-247
CGGTC 735735 0.97567344 6.9231863 130-139
AGCTT 514120 0.97440314 5.7492576 150-159
GCTTC 619125 0.97071445 5.481295 150-159
CATCA 398165 0.9651804 5.734451 230-239
GTCAG 598610 0.9595952 5.228987 130-139
ACTAA 323325 0.94742686 11.129545 240-247
ACCCA 411495 0.94362646 11.388007 240-247
CGGAG 694150 0.9411685 6.3742785 210-219
GAGTT 551430 0.934425 5.0667276 160-169
ACCGT 513530 0.9207253 12.769471 45-49
TGAAC 418095 0.90615034 5.492859 190-199
CTACC 451615 0.9056341 6.7009277 230-239
CTTCC 507695 0.89029956 5.039838 230-239
TGAAG 452870 0.87756383 8.9517 35-39
ATCAA 295515 0.8659363 12.193051 70-79
TGCAT 455315 0.8629509 6.331306 210-219
CCAGC 508160 0.8618959 5.457143 80-89
GAGCT 537015 0.8608561 5.2952347 140-149
GGAAG 525170 0.86074597 9.229729 30-34
AACTT 334305 0.8566397 5.094112 200-209
GTATG 501440 0.84971446 5.129019 220-229
TGCGT 601565 0.8432872 6.7874255 210-219
AGCTG 525525 0.8424372 6.463399 30-34
ACGTC 460185 0.8250812 7.3319173 70-79
CCTTT 434010 0.8045312 6.104603 50-59
AATCC 327835 0.7946955 10.309704 240-247
AAAGG 358530 0.7944778 7.1943674 10-14
AGCCA 377985 0.774979 7.035697 80-89
CTAAT 300410 0.7697855 8.989697 240-247
TAACC 316880 0.7681397 6.515791 80-89
TGAGA 389760 0.7552703 5.7455935 240-247
CCCAT 365500 0.7329456 5.0029607 240-247
ATACC 302225 0.732615 6.522893 240-247
TGGAT 430685 0.7298167 7.0293326 10-14
CAATC 299610 0.72627604 10.213536 240-247
TCGAA 333785 0.7234227 12.699137 70-79
GACCG 476990 0.7233417 12.909191 45-49
TAGGT 426325 0.72242844 6.0644155 10-14
AGGAT 372580 0.72197926 7.9885554 10-14
GGTCC 524280 0.6952586 8.915218 50-59
GTCGA 425200 0.6816122 8.834779 70-79
AATGG 345235 0.6689906 5.458248 10-14
CGAAC 325775 0.6679334 9.0346985 70-79
ACGAT 300055 0.6503186 8.070018 10-14
GTAGC 397140 0.63663095 5.002498 80-89
CTTTG 383565 0.6357143 5.3019037 50-59
AAGGT 325935 0.6315914 6.809053 10-14
AAAGC 249960 0.61950797 8.634713 70-79
CATCC 307615 0.6168675 7.5883293 240-247
ATAGG 304825 0.5906847 5.2457933 10-14
CGTCC 396590 0.5882271 5.891561 70-79
GAAGC 309620 0.56757635 8.385208 30-34
TTGCC 351430 0.5510004 6.141948 60-69
TCAAG 249415 0.54056495 5.688841 70-79
AAGCT 240050 0.5202679 13.205313 30-34
GACGA 277275 0.5082835 6.161508 40-44
CGGTT 354270 0.49662358 10.050934 10-14
AAGCA 193720 0.48012123 5.4970713 70-79
GGGAT 333035 0.47732463 5.755034 10-14
GAATA 168625 0.44178233 5.0525894 6
TCGAT 219645 0.41628954 6.76923 10-14