FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_13_SL254623.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_13_SL254623.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4213129
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 4470600 7.3184185 50.715843 110-119
CACAG 6351420 6.822582 35.671043 110-119
AAAAA 3145500 6.2987685 38.89237 230-239
TCCCT 6468005 6.2583604 88.21061 30-34
CAGTA 5003030 5.7899413 37.41183 60-69
GGAGA 6425205 5.6847477 84.77057 35-39
ATACA 3709130 5.6358624 27.176657 110-119
GGTGA 6958065 5.566156 86.51885 45-49
CCCTG 6539365 5.330106 52.637295 30-34
AGTAA 3748095 5.168589 37.745533 110-119
GTAAT 4009570 4.999217 33.652733 110-119
ACAGT 4277765 4.950601 30.323347 110-119
GGCCC 6893420 4.7330923 53.570324 60-69
GTAGT 4897790 4.6511145 23.35629 70-79
GAGAC 4737025 4.61803 115.29122 40-44
ACTCC 4256625 4.5552564 91.58165 30-34
CCCCA 4550210 4.519765 89.42602 60-69
GTGAC 5117965 4.5111895 77.02756 45-49
AGACG 4612020 4.4961653 88.93732 40-44
GTGTC 5326425 4.2449474 24.196445 120-129
CCCTT 4284870 4.145986 63.27742 20-24
GCACA 3842000 4.1270075 29.522684 110-119
CGTGT 5139560 4.0960236 23.213598 120-129
ACCAG 3812085 4.0948734 85.97301 45-49
TGGTG 5543595 4.0096006 29.053232 25-29
CACTC 3723885 3.9851415 78.567444 25-29
TGACC 3985545 3.8708677 139.37509 45-49
AATAC 2524045 3.8351765 27.195984 110-119
TAGTA 3048175 3.800529 24.550863 80-89
CCCAG 4198355 3.784745 75.33416 60-69
CTCAG 3868080 3.7567828 27.500124 140-149
TGAGG 4696060 3.7566483 66.12498 35-39
CCACT 3445845 3.6875951 24.799747 240-247
GACCA 3429595 3.6840098 96.329765 45-49
CTTGG 4565295 3.6383572 38.511955 20-24
AGTAG 3407220 3.5786097 24.753717 70-79
CTCCC 3968430 3.5640664 53.780785 30-34
TGTCT 3732320 3.5310624 20.170341 180-189
CAGGG 4718655 3.5036604 31.254934 50-59
CTGAG 3946115 3.4782717 90.74701 35-39
TCTTG 3643320 3.4468615 18.85386 170-179
GACGG 4630125 3.4379258 103.83011 40-44
AGGAG 3876820 3.4300454 84.19311 35-39
CTTCA 2974750 3.4297392 16.391014 200-209
GCCCC 4490625 3.3973784 67.959946 60-69
ATGGT 3560385 3.3810675 23.701374 190-199
ACGGT 3776165 3.3284707 87.869194 40-44
AGGCT 3710135 3.270269 42.151672 30-34
CCTGG 4374440 3.2359054 30.864807 50-59
AGATA 2339410 3.2260253 16.46791 150-159
GGGTT 4458585 3.224829 41.892418 50-59
GCCCT 3937195 3.209129 32.88994 20-24
TGTCC 3644485 3.200365 18.495478 120-129
ATGCA 2762910 3.1974795 76.45872 25-29
TTCCC 3294700 3.1879103 53.073883 50-59
AGTCA 2741955 3.1732285 26.51381 70-79
GATGG 3914005 3.1310377 32.446045 15-19
CTCTC 3229785 3.1250992 13.07949 100-109
GAGGA 3507970 3.1037025 116.373405 35-39
CCAGT 3187310 3.0956006 37.88208 60-69
CCAGG 3778885 3.0916781 36.181774 45-49
TCAGG 3501200 3.0861049 19.01689 140-149
GGATG 3849345 3.0793123 58.419197 20-24
TGCAC 3111975 3.0224333 101.260735 25-29
CCGTG 4046305 2.993174 22.638792 120-129
TCACA 2323430 2.962767 11.334685 140-149
TCAAA 1949620 2.962363 48.502068 70-79
GGTTC 3708950 2.9558847 46.671528 50-59
TGGAG 3671675 2.9371839 31.820328 25-29
TCTCA 2459770 2.8359928 19.16578 130-139
TCTCT 2709335 2.824334 14.937986 180-189
TTCTT 2503165 2.8112917 21.009829 170-179
GCCGT 3799865 2.8108752 21.632421 120-129
GGTTG 3881830 2.8076706 62.24028 15-19
TCGCA 2878670 2.7958417 22.700068 100-109
CGGAT 3154885 2.7808483 65.028915 20-24
ACAGA 2166260 2.7727382 12.634755 140-149
TGGCC 3733390 2.7617016 36.80521 60-69
GACTC 2840525 2.7587943 19.048098 190-199
GCACT 2838690 2.7570117 72.05593 25-29
TACAC 2159095 2.753212 19.967768 110-119
CAGAT 2372425 2.7455766 14.267977 150-159
AGGGT 3374150 2.699177 47.74711 50-59
CGCAC 2986510 2.692288 22.73426 100-109
GTTCC 3037530 2.6673741 52.499847 50-59
CGGTG 3924105 2.6344328 65.912346 40-44
GGCTG 3872940 2.6000834 24.522928 30-34
GTTGG 3581650 2.590555 48.407227 15-19
AGGGA 2914930 2.579006 8.909547 150-159
ACCAC 2174055 2.5732105 13.322166 230-239
CCTGA 2645825 2.569696 60.808228 30-34
GATGC 2800410 2.4683993 75.278206 25-29
CGGCC 3584115 2.4608896 10.064514 120-129
TGTTC 2545580 2.408315 12.643106 140-149
AATAA 1328470 2.405251 11.278821 110-119
CTGGC 3249270 2.4035835 32.776665 60-69
GTCTC 2727845 2.3954275 11.9425955 180-189
TCCTC 2461155 2.3813827 14.01884 130-139
AGATG 2260775 2.3744967 15.741645 180-189
GAGAT 2223300 2.3351367 15.247241 180-189
ACTCT 2008045 2.315176 12.136316 200-209
TTGTC 2430090 2.2990525 14.706937 170-179
GTCAA 1975190 2.2858615 35.984535 70-79
GGCGG 4037920 2.2835696 37.55052 20-24
GTTCT 2410780 2.2807837 18.044397 160-169
TTGGT 2619940 2.249526 42.08481 25-29
TCTGC 2550415 2.2396193 11.120822 210-219
CTGGA 2525305 2.2259102 14.094326 180-189
CATTT 1789225 2.2224844 16.280716 150-159
CAGAG 2271820 2.2147515 8.585178 140-149
TCATT 1777895 2.208411 15.101907 150-159
GCGGA 2973140 2.2075934 77.20176 20-24
TTCAT 1773500 2.2029517 16.471796 140-149
TTCAC 1902590 2.1935918 16.26113 200-209
TCTCG 2462045 2.1620183 15.918565 100-109
TGTAG 2267925 2.153702 8.427822 210-219
GATAT 1720580 2.1452556 9.311708 180-189
TCCAT 1844585 2.1267147 11.706604 70-79
TTGGG 2923120 2.11425 76.40769 15-19
ACAGC 1958200 2.1034634 11.003046 110-119
CTCTG 2394685 2.1028666 14.310397 180-189
TTGGA 2187055 2.0769053 15.826285 170-179
GTGAA 1975135 2.0744882 15.665381 190-199
TGAAT 1658010 2.0672421 13.768578 190-199
ATTTG 1829720 2.0626783 14.5748825 150-159
GAGTC 2327165 2.0512612 11.83791 210-219
GAACT 1772115 2.0508454 12.400157 160-169
GTCTG 2571255 2.0491872 8.810457 210-219
TAGTC 1954040 2.0446417 22.955002 70-79
CCTTG 2327840 2.0441675 42.935516 20-24
GTCAC 2101810 2.0413342 8.948232 130-139
CCTTC 2108255 2.039921 13.125916 200-209
GTTCA 1934565 2.0242639 14.6236315 140-149
GATAC 1731375 2.0036976 12.243231 150-159
CACTA 1569495 2.001372 12.782604 240-247
GAATC 1725755 1.9971938 13.892584 190-199
CTCCA 1861645 1.9922522 7.6857524 240-247
ATATA 1213420 1.986381 9.33912 110-119
CAGCC 2187300 1.9718137 10.17944 120-129
TCGGC 2644580 1.9562759 8.332746 190-199
AATAT 1185395 1.9405038 11.062342 110-119
TCAGC 1996615 1.9391658 10.572459 140-149
CTACT 1662370 1.91663 7.590438 230-239
CTCGC 2350280 1.9156662 15.934982 100-109
ACTAC 1490215 1.9002765 11.009371 230-239
TTCAG 1813780 1.8978784 9.775012 150-159
ATATG 1519950 1.8951057 9.051595 180-189
GTTGT 2206295 1.8943633 7.558966 210-219
TGGGG 3089770 1.8825468 45.650032 15-19
CCAGA 1746235 1.8757743 21.34164 60-69
CACCA 1578620 1.8684539 9.684941 230-239
TTTGC 1972610 1.8662411 11.49178 150-159
TTGCA 1761630 1.8433104 12.888697 150-159
ATAGA 1336440 1.8429388 9.0120535 240-247
ACACA 1306205 1.842199 11.711641 110-119
AGTCT 1758170 1.83969 12.334242 210-219
GCTGT 2306690 1.8383396 9.642623 140-149
AATCG 1580570 1.8291728 12.590707 190-199
ATCTC 1576795 1.8179665 6.4479704 100-109
GTCCT 2068865 1.8167517 12.147053 130-139
AATAG 1317420 1.8167104 7.557089 240-247
TCCCA 1696245 1.8152483 10.530266 240-247
GCTGA 2056940 1.8130735 36.55296 30-34
TACAG 1562190 1.807902 10.553493 160-169
CACGG 2202465 1.8019371 6.932435 200-209
ATTGT 1583185 1.784755 8.047568 170-179
GGTCA 1995565 1.758975 9.292563 130-139
CTGTT 1852510 1.7526172 12.212334 140-149
TGGAA 1667500 1.7513784 8.593253 25-29
CAGGC 2114595 1.7300469 12.824164 140-149
AGTAT 1384190 1.7258375 10.946858 220-229
TACCA 1331655 1.6980857 10.503834 230-239
GGGGC 3001190 1.6972666 41.553703 15-19
TACTA 1227880 1.6868907 9.650966 230-239
GTGGA 2106565 1.6851624 38.970592 25-29
TCTGG 2108485 1.6803781 11.870792 180-189
TTGTA 1480795 1.6693286 9.5195875 210-219
ATCGG 1885980 1.6623821 9.127081 190-199
CATAG 1432470 1.6577787 7.0824685 210-219
GGAGG 2440980 1.6449063 26.19485 30-34
CCCAC 1649195 1.6381602 9.629647 240-247
ATGTA 1312860 1.6369015 10.044867 70-79
ACACG 1512745 1.6249636 8.484823 110-119
CAGCT 1665300 1.6173841 8.5108595 150-159
TGCAG 1831860 1.6146784 11.996577 150-159
GGGCG 2845745 1.6093575 48.72845 20-24
ATAGT 1290345 1.6088295 6.4007583 220-229
CTCTT 1535215 1.6003779 8.869189 200-209
TGGTT 1838530 1.5785936 17.09301 10-14
GCAGA 1609180 1.5687569 12.285199 150-159
CTTGA 1496640 1.5660337 9.368887 200-209
TGACT 1495105 1.5644275 11.363134 190-199
ATGGG 1938745 1.5509135 53.208958 15-19
GACGT 1746215 1.5391873 9.329237 170-179
ACGGC 1863750 1.524819 11.106499 120-129
AGAAC 1190315 1.5235622 9.524026 160-169
CAAAG 1188045 1.5206567 24.419367 70-79
CCACC 1526140 1.5159285 7.0803256 230-239
GTCCC 1854350 1.5114437 16.029348 50-59
CACTG 1547635 1.5031044 6.0550833 230-239
GGACG 2019960 1.4998456 7.3177643 170-179
ATAAT 915280 1.4983228 5.1205335 110-119
GCTCT 1689650 1.4837481 8.914896 130-139
ACTGA 1280735 1.4821777 6.7633057 180-189
CTGGT 1857775 1.4805722 6.6612964 160-169
CGACT 1511415 1.4679266 8.861499 190-199
CTGAA 1265650 1.4647201 12.894424 35-39
GAGAA 1255635 1.4585956 9.438807 160-169
AACTG 1259475 1.4575739 9.577983 160-169
AGCTC 1497310 1.4542277 8.764706 140-149
CAAAA 864580 1.4529502 17.242697 70-79
GAGGG 2152650 1.450609 8.806024 200-209
TCCAG 1475345 1.4328945 5.09654 240-247
AACCA 1015205 1.4317888 10.605275 70-79
ATAAA 789845 1.4300476 5.680332 110-119
GGAAT 1360660 1.4291041 8.1975565 170-179
TCGAC 1466295 1.4241049 9.268243 190-199
GAAGA 1225150 1.4231828 16.624796 35-39
ACGGA 1457960 1.4213357 9.015778 200-209
TGGAC 1606280 1.4158428 10.125891 170-179
CCTCG 1724830 1.4058745 8.464585 130-139
GAGGC 1876130 1.3930502 22.527239 30-34
CGTAG 1579020 1.3918146 7.828282 210-219
TCAGA 1200955 1.3898494 7.8025227 130-139
TCCAC 1298675 1.389786 9.542062 240-247
CAGAC 1290845 1.3866026 11.033526 60-69
AGATC 1185020 1.3714082 6.445631 140-149
AGCCG 1644110 1.3451216 9.892597 120-129
GGCCG 2150890 1.3402987 11.03926 120-129
CCATC 1248720 1.3363265 9.880714 240-247
CTCGA 1374595 1.3350434 8.449476 190-199
GGGAG 1978390 1.3331802 6.441858 150-159
AATTG 1064050 1.32668 8.512549 170-179
TAGTT 1176425 1.3262064 7.858197 220-229
GGCTC 1788540 1.3230373 7.8446207 130-139
ATCCA 1037475 1.3229563 14.856347 240-247
GGTGG 2149390 1.3095886 39.73673 25-29
AAGAG 1118010 1.2987247 11.533282 35-39
GTGTT 1509945 1.2964648 7.1643486 160-169
TATGG 1357665 1.2892867 6.657283 180-189
GAATT 1017620 1.2687904 9.435221 170-179
AGAGC 1301005 1.2683234 7.1251802 140-149
CATGT 1198490 1.2540597 9.637173 70-79
GTGCT 1569895 1.2511435 7.1626205 220-229
ACTAT 910440 1.2507842 6.64488 230-239
CCTCA 1168615 1.2506014 5.46901 130-139
CAATA 822385 1.2495784 6.6721697 100-109
GGGCC 2002110 1.2475884 30.340086 15-19
TTCCA 1075515 1.2400155 7.157989 230-239
TGGGC 1845070 1.2386806 33.09186 15-19
ATATC 898925 1.2349645 5.6023016 70-79
ACCAT 963620 1.2287787 9.6365185 45-49
ACTCG 1253665 1.217593 8.723039 190-199
AGAGA 1044035 1.2127925 11.7317915 35-39
ACTTC 1049385 1.2098888 5.003532 220-229
ACGTG 1370950 1.208413 8.885129 170-179
GGAGT 1497435 1.1978846 6.4484863 210-219
GCGTA 1357210 1.1963019 8.623196 210-219
GTCCA 1226005 1.190729 10.810401 70-79
TGATA 952380 1.1874474 6.3486676 180-189
CCATG 1215905 1.1809194 9.612736 70-79
GCTCA 1213640 1.1787198 8.563444 140-149
TTGAG 1238280 1.1759145 8.21293 200-209
TGTGC 1475205 1.1756794 5.848488 220-229
TATAC 852950 1.1718031 5.828136 110-119
AAGTA 845240 1.1655784 15.025231 70-79
TATCA 839220 1.1529405 5.45065 70-79
AAAGT 832240 1.1476514 18.382126 70-79
CTGAT 1092330 1.1429774 5.615932 180-189
TAGAT 915685 1.1416955 7.494095 240-247
AGTTC 1081815 1.1319748 5.50663 160-169
CTTCT 1082960 1.1289268 5.1776323 210-219
CTGCG 1519025 1.1236688 6.070081 210-219
CCCAA 938420 1.1107135 8.309372 240-247
TGAAC 957480 1.1080791 5.217939 190-199
AGGTT 1164795 1.1061306 19.17323 10-14
GAACC 1024215 1.1001934 8.01618 70-79
GTCTA 1050205 1.0988992 7.2754316 180-189
ACTGG 1238975 1.0920846 6.4895353 160-169
TGCCC 1335655 1.0886657 9.029966 60-69
AAAAG 713550 1.088286 11.503197 70-79
ACCCA 916060 1.0842482 10.321898 240-247
TCACG 1097945 1.0663536 8.014199 200-209
TATGT 938065 1.0574987 6.874113 220-229
GCGGT 1571060 1.054725 6.7285423 130-139
GGAAG 1180185 1.0441774 9.366294 30-34
ACAAT 684710 1.0403872 7.501148 100-109
GCATA 896205 1.0371662 6.6008687 210-219
CGGCT 1397155 1.0335177 7.1112227 130-139
GTGGT 1423140 1.0293361 7.33019 50-59
CCAAT 802610 1.0234637 9.789013 240-247
CTCGG 1378680 1.0198512 7.0988884 130-139
TCTAC 884520 1.0198077 6.787094 180-189
ACGTC 1048740 1.0185645 7.145454 70-79
TGGTC 1260765 1.0047792 8.218162 50-59
CCAGC 1113945 1.0042024 5.4263873 80-89
GTATG 1052510 0.99950075 5.3563313 220-229
TCCCC 1099370 0.98734957 5.5711207 90-99
TTCTG 1025190 0.9699088 5.1609406 210-219
TATAA 587570 0.9618582 6.542637 240-247
ATAAC 625415 0.95029086 6.055263 80-89
CATCA 742585 0.94692165 5.096791 230-239
CGATG 1071835 0.94476044 21.922113 15-19
CGGTC 1271155 0.94031173 6.705648 130-139
CGGAG 1255000 0.93185323 5.9099374 210-219
GCTTC 1058045 0.92911077 5.2295413 150-159
GAGTT 978375 0.92909956 5.079449 160-169
TGTAA 737940 0.92007923 5.892932 230-239
AGCTT 872815 0.91328424 5.4893336 150-159
TGAAG 869415 0.9131482 9.38314 35-39
ACCGT 924920 0.898307 12.648965 45-49
GACCG 1070110 0.875506 12.6415205 45-49
ACTAA 571365 0.86816436 9.835273 240-247
AATCC 679015 0.86585915 9.484496 240-247
AGCTG 953830 0.8407459 6.7736616 30-34
TGCGT 1050495 0.8372024 6.7595177 210-219
TGCAT 798780 0.83581656 5.4704685 210-219
CTACC 778660 0.8332884 6.104462 230-239
AAAGG 717305 0.8332499 7.5553093 10-14
CCTTT 796240 0.83003676 6.2153044 50-59
ATCAA 544335 0.8270934 11.338518 70-79
GAGCT 935255 0.82437307 5.095314 140-149
AGCCA 714400 0.76739573 6.4793305 80-89
TGAGA 724065 0.76048684 5.3668685 240-247
TAGGT 774565 0.7355543 6.04441 10-14
CAATC 555360 0.7081781 9.316588 240-247
TGGAT 742750 0.70534164 6.973255 10-14
TAACC 552900 0.70504117 5.7467003 80-89
AGGAT 669415 0.703088 8.103309 10-14
CTAAT 510535 0.70138514 8.081644 240-247
GGTCC 938840 0.6944884 8.976897 50-59
AAGGT 646555 0.6790779 6.707715 10-14
TCGAA 586730 0.6790149 11.626459 70-79
ATACC 522695 0.66652465 5.390945 240-247
CTTTG 690830 0.6535784 5.499736 50-59
GTCGA 736320 0.6490235 8.168046 70-79
AATGG 606385 0.6368873 5.463586 10-14
ACGAT 548700 0.6350033 8.433475 10-14
CGAAC 565710 0.6076755 7.9230704 70-79
CATCC 565575 0.605254 6.3935876 240-247
AAAGC 469930 0.6014942 8.057869 70-79
CGTCC 713865 0.58185714 5.752804 70-79
GAAGC 590010 0.5751888 8.44141 30-34
TTGCC 653445 0.57381564 6.080467 60-69
ATAGG 533105 0.5599212 5.065994 10-14
AAGCT 453835 0.5252173 13.42135 30-34
TCAAG 450455 0.5213057 5.232714 70-79
CGGTT 635520 0.506484 10.541616 10-14
GGGAT 621730 0.49735764 6.473881 10-14
TGGGT 667335 0.48267353 5.092 10-14
GACGA 493510 0.48111293 5.6088834 40-44
AAGCA 357415 0.45747888 5.0556664 70-79
TCGAT 411705 0.43079427 6.800662 10-14
GCGAT 358000 0.31555626 5.1802683 10-14