FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_9_SL254636.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_9_SL254636.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9570373
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11125415 8.35283 58.659756 110-119
CACAG 14258770 7.098704 40.92618 110-119
CAGTA 12323035 6.4639835 42.607628 60-69
TCCCT 15365150 6.427177 92.046684 30-34
ATACA 8898120 6.340596 31.561384 110-119
GGAGA 15442740 6.152885 93.94542 35-39
AGTAA 9183150 5.8539934 43.67896 110-119
GGTGA 16874685 5.829049 92.57712 45-49
CCCTG 15675505 5.5673447 55.343155 30-34
GTAAT 9925520 5.4855657 37.738213 110-119
AGACG 11276760 5.0223856 100.42643 40-44
GGCCC 16649285 5.020712 58.840725 60-69
ACAGT 9538035 5.003126 34.31511 110-119
GTGAC 12681355 4.8966494 83.21224 45-49
GAGAC 10811750 4.8152814 130.09253 40-44
CCCCA 10486945 4.802189 102.118256 60-69
GTAGT 11802665 4.8017435 24.358562 70-79
AATAC 6245275 4.4502397 31.902416 110-119
GTGTC 13226195 4.4276767 25.371271 120-129
ACCAG 8893480 4.4276032 99.28263 45-49
CGTGT 12684325 4.246277 24.454084 120-129
TGACC 9837640 4.246152 153.082 45-49
GCACA 8471030 4.2172875 34.150734 110-119
CCCTT 9997725 4.182006 65.32624 20-24
ACTCC 8624190 4.160966 90.58346 30-34
GACCA 8260590 4.11252 111.14132 45-49
TAGTA 7282450 4.024812 26.044052 80-89
TGGTG 13333395 3.9931023 28.73403 25-29
CTCAG 9168080 3.9571538 29.78328 140-149
CCCAG 9647730 3.9522474 84.26279 60-69
CCACT 8167980 3.9408553 27.585388 240-247
AGTAG 8237085 3.8653145 27.720217 70-79
CACTC 7914710 3.8186588 78.23476 25-29
TGAGG 11011135 3.8035936 69.71099 35-39
GACGG 11345895 3.7197623 113.2589 40-44
CTTGG 11009515 3.6856074 38.442898 20-24
AGGAG 9201065 3.6660006 93.39826 35-39
CTCCC 9226970 3.663169 56.512745 30-34
CAGGG 11140005 3.6522613 34.39252 50-59
CTGAG 9446515 3.6475806 97.44615 35-39
ACGGT 9319720 3.5986216 94.95863 40-44
ATGGT 8802260 3.5810723 25.423983 190-199
GCCCC 10619210 3.5795894 74.94181 60-69
TGTCT 8895380 3.507214 20.797693 180-189
AGGCT 9067350 3.5011742 45.776817 30-34
TCTTG 8819895 3.477452 19.359226 170-179
AGATA 5382045 3.4308987 18.762724 150-159
GAGGA 8555520 3.4087946 129.7806 35-39
GCCCT 9595855 3.4080837 34.099262 20-24
CCAGG 9174475 3.3622415 40.70383 45-49
TGTCC 8953675 3.3505301 19.326868 120-129
TTCCC 7750685 3.2420788 56.75164 50-59
CTTCA 6375665 3.2410588 16.931166 200-209
CCTGG 10176475 3.23335 32.7853 50-59
TCAGG 8362580 3.229041 20.040386 140-149
GATGG 9328995 3.2225294 33.455135 15-19
CTCTC 7675035 3.2104347 13.998152 100-109
ATGCA 6114805 3.207489 76.888596 25-29
GGGTT 10584635 3.1698997 43.77531 50-59
CCGTG 9967440 3.1669338 24.58811 120-129
ACAGA 5090595 3.079949 15.014035 140-149
TCGCA 7100145 3.0645857 24.836002 100-109
CGCAC 7414620 3.0374413 25.514456 100-109
GGATG 8744160 3.020509 55.6653 20-24
TGGAG 8719145 3.0118682 32.432495 25-29
GGTTC 8951585 2.9966834 49.49683 50-59
TCTCA 5891680 2.9950259 21.507917 130-139
GACTC 6877815 2.968623 21.159166 190-199
CCAGT 6868940 2.9647923 42.066143 60-69
TGCAC 6837130 2.9510622 100.11051 25-29
TACAC 5030130 2.9493954 22.560793 110-119
TGGCC 9255495 2.940729 39.406883 60-69
TCTCT 6667725 2.9386423 15.695324 180-189
CAGAT 5509405 2.8899295 15.8100395 150-159
GTTCC 7621540 2.8520355 56.45012 50-59
AGGGT 8154050 2.8166664 51.94984 50-59
TTCTT 6003720 2.7878864 21.269688 170-179
TCAAA 3896880 2.7768273 57.619152 70-79
CCTGA 6430240 2.7754393 65.40342 30-34
CGGTG 9745620 2.7700875 68.7968 40-44
GCCGT 8715695 2.7692194 23.361094 120-129
AATAA 3184075 2.7573574 13.392571 110-119
TCACA 4631885 2.7158864 12.375154 140-149
ACCAC 4861330 2.7053494 15.620478 230-239
GCACT 6259880 2.7019083 70.98247 25-29
CGGAT 6972755 2.6923885 63.191208 20-24
CGGCC 8871970 2.6754065 11.418049 120-129
GGCTG 9327150 2.651142 25.631996 30-34
GGTTG 8831075 2.6447413 56.603992 15-19
AGTCA 5010590 2.628279 30.09858 70-79
AGGGA 6546625 2.6083863 9.676539 150-159
AGATG 5474015 2.5687232 17.235126 180-189
CTGGC 7899490 2.5098882 35.143578 60-69
TGTTC 6303450 2.4852846 13.064001 140-149
GAGAT 5226935 2.4527788 16.852987 180-189
GTTGG 8125875 2.4335473 44.286865 15-19
ACTCT 4723880 2.4013767 13.706147 200-209
GATGC 6217165 2.4006326 72.53025 25-29
GTCTC 6334275 2.3703315 12.538859 180-189
TCATT 4368435 2.3397684 16.491257 140-149
TTGTC 5932500 2.3390288 15.27796 170-179
TCCAT 4579425 2.327943 12.037573 70-79
CTGGA 6021805 2.3251982 15.332764 180-189
CAGAG 5214690 2.3224916 9.590684 140-149
TTCAT 4335830 2.3223052 17.417618 140-149
CATTT 4335470 2.3221123 17.23895 150-159
ACAGC 4660350 2.320147 12.555107 120-129
GTTCT 5839070 2.3021915 18.316988 160-169
TCCTC 5451615 2.2803874 14.824815 130-139
TGTAG 5543180 2.2551627 8.56619 210-219
TTGGT 6295175 2.2204158 41.55705 25-29
TTCAC 4323920 2.198058 17.672808 200-209
CACTA 3742720 2.194528 14.385853 230-239
GTGAA 4663625 2.1884415 17.056559 190-199
GGCGG 9015015 2.175673 35.45362 20-24
ATATA 2888940 2.168982 10.858663 110-119
TAGTC 4768050 2.1683578 24.569094 70-79
ATTTG 4453745 2.134033 15.188736 150-159
GAATC 4064820 2.1321802 15.446117 190-199
TGAAT 3855230 2.130681 14.722497 190-199
AATAT 2832155 2.1263485 12.63597 110-119
GAGTC 5497125 2.122604 12.616548 210-219
CTCTG 5669545 2.121585 15.152746 180-189
GCGGA 6445255 2.113083 75.88391 20-24
GTCAA 4006385 2.1015282 41.660713 70-79
CAGCC 5116185 2.0958743 11.518991 120-129
ACTAC 3570740 2.0936882 12.341914 230-239
TTGGA 5125680 2.0853088 17.183722 170-179
TCTGC 5527320 2.068363 10.996164 210-219
CTCGC 5820000 2.0670433 17.049042 100-109
GATAC 3938060 2.0656888 13.638394 150-159
GTTCA 4540585 2.0649142 15.761092 140-149
AATAG 3236655 2.063274 8.66211 240-247
TCGGC 6478580 2.0584254 8.754085 190-199
CCTTG 5487220 2.0533576 43.414433 20-24
TCTCG 5449965 2.039416 16.89979 100-109
CCTTC 4839770 2.0244553 13.824899 200-209
ATAGA 3171875 2.0219789 10.243071 240-247
ATATG 3650785 2.01769 9.363563 180-189
GCTGA 5194015 2.005564 40.42487 30-34
AATCG 3822860 2.0052612 14.014801 190-199
CACGG 5466485 2.0033455 7.843221 200-209
CTACT 3933265 1.9994688 8.231406 230-239
TACAG 3782725 1.9842086 11.746912 160-169
ACACA 2893245 1.9567324 13.726233 110-119
TCCCA 4034160 1.9463859 11.553401 240-247
TTGGG 6467795 1.9369833 70.338715 15-19
TGGAA 4103485 1.9255916 9.715416 25-29
GATAT 3477115 1.9217072 9.164639 180-189
TTGCA 4198010 1.909122 13.465874 150-159
GCTGT 5671200 1.8985231 9.988784 140-149
GTCCT 5064715 1.8952531 12.629255 130-139
TACCA 3230690 1.8943014 12.033567 230-239
GTTGT 5367200 1.8931031 7.5167456 210-219
GGTCA 4892540 1.8891555 9.784908 130-139
ATTGT 3934590 1.8852774 8.6299715 170-179
AAAAA 1878845 1.8766925 6.862272 230-239
TACTA 3022810 1.8674557 10.821505 230-239
TTTGC 4726495 1.8635323 11.652398 150-159
AGTAT 3370120 1.8625739 11.715332 220-229
CAGGC 5052790 1.8517355 14.240494 140-149
CATAG 3524765 1.848897 8.350358 210-219
AGTCT 4024200 1.8300786 13.085579 210-219
GTCAC 4220465 1.8216497 9.435165 140-149
GAACT 3455270 1.812444 11.31169 160-169
ATGTA 3268030 1.8061514 10.404985 70-79
CTGTT 4574495 1.8036027 12.602117 140-149
ATAGT 3181080 1.7580967 7.121881 220-229
GTGGA 5036875 1.7398956 40.100212 25-29
TTGTA 3618610 1.7338742 9.679883 210-219
GTCTG 5156225 1.7261271 8.942457 210-219
TCAGC 3997415 1.7253761 11.201904 140-149
TCTGG 5145865 1.7226588 12.392238 180-189
TGGGG 6714135 1.7072728 42.25145 15-19
ATCTC 3356775 1.7064111 7.4786134 100-109
CACCA 3050795 1.6977795 11.342427 230-239
ATAAT 2248650 1.6882598 6.0536633 100-109
AGAAC 2785610 1.6853701 11.430744 160-169
GCAGA 3780925 1.6839288 13.827183 150-159
ACGGC 4592010 1.6828698 12.763751 120-129
CTCCA 3451555 1.6652929 8.610402 240-247
TGACT 3658390 1.6637199 12.522484 190-199
CAGCT 3840335 1.657577 9.532412 150-159
GGAGG 5647375 1.6563488 27.620007 30-34
CCCAC 3616890 1.6562487 10.621077 240-247
TGCAG 4250620 1.641291 12.70243 150-159
TTCAG 3590865 1.6330115 10.106314 150-159
TCAGA 3088110 1.6198518 9.506466 130-139
CAAAG 2669230 1.614957 30.130836 70-79
GACGT 4174140 1.6117599 10.286088 170-179
CCAGA 3211880 1.5990286 23.035362 60-69
GAGAA 2953510 1.5986086 11.036734 160-169
GGACG 4867380 1.5957751 8.326974 170-179
CACTG 3694210 1.5945061 6.8218107 230-239
ATAAA 1838625 1.5922195 6.4609313 110-119
AACCA 2345435 1.5862427 12.0056505 70-79
GGGGC 6568505 1.5852352 39.11281 15-19
AACTG 2996650 1.5718772 10.804547 160-169
ACGGA 3529155 1.5717969 10.4326515 200-209
CAAAA 1905805 1.5664 20.416307 70-79
GTCCC 4391300 1.5596232 16.533354 50-59
CGACT 3594015 1.5512596 9.498495 190-199
TGGTT 4396680 1.5507843 16.387594 10-14
ATGGG 4487315 1.5500603 54.877987 15-19
TCGAC 3564125 1.5383582 10.099585 190-199
AGCTC 3551725 1.5330061 9.721618 140-149
GGGCG 6302560 1.5210524 45.56813 20-24
CTCTT 3437915 1.5151799 9.466124 200-209
ACTGA 2884890 1.5132539 7.480218 180-189
CCTCG 4208280 1.4946215 9.061263 130-139
AAATA 1707390 1.4785721 5.178542 110-119
TCCAC 3061080 1.4768981 10.30299 240-247
CTGGT 4368190 1.46232 6.9805465 160-169
AGCCG 3971440 1.4554447 10.910051 120-129
GCTCT 3887535 1.4547437 9.496368 130-139
CCATC 3014740 1.4545401 11.480115 240-247
GGCCG 5349180 1.4430642 12.135032 120-129
TGGAC 3735680 1.4424574 11.5689535 170-179
ACACG 2891250 1.4394038 9.390333 110-119
CTCGA 3324615 1.4349802 9.138469 190-199
AATTG 2593625 1.4334261 9.535822 170-179
ATCGG 3705535 1.4308175 9.582123 190-199
CAATA 2001805 1.4264405 7.8374763 100-109
CCACC 3105070 1.4218757 8.217712 230-239
GAGGG 4790115 1.4049184 9.260275 200-209
TAGTT 2910700 1.3946755 8.812093 220-229
CGTAG 3539405 1.3666698 8.413003 210-219
ACTAT 2197070 1.3573233 7.3028173 230-239
GAATT 2449415 1.3537253 10.57123 170-179
CTTGA 2967265 1.3494179 9.543641 200-209
ACCAT 2286460 1.3406563 10.504735 45-49
CATGT 2946800 1.3401111 9.753 70-79
GGGAG 4565970 1.3391776 6.7722287 150-159
AAGTA 2089050 1.3317091 17.895134 70-79
GAGGC 4054430 1.3292489 24.325176 30-34
TATGG 3262980 1.3274963 6.629876 180-189
ACTCG 3057035 1.3194867 9.58045 190-199
GGCTC 4135735 1.3140383 8.563231 130-139
AAAGT 2052605 1.3084764 22.201654 70-79
CAGAC 2621995 1.3053557 11.756849 60-69
TTCCA 2561800 1.302287 7.5222116 230-239
ACTTC 2557230 1.2999637 5.8411813 220-229
ATCCA 2216970 1.299911 17.098255 240-247
GTGCT 3880040 1.2989043 7.4279823 220-229
GGTGG 5053725 1.2850629 39.774525 25-29
ACTGT 2817970 1.2815233 5.4185185 230-239
CCATG 2968085 1.2810936 9.785206 70-79
ACGTG 3314410 1.2797925 9.754189 170-179
GGGCC 4723270 1.2742106 31.178831 15-19
GTGTT 3577645 1.2618966 7.482398 160-169
TGTGC 3760310 1.2588227 6.359364 220-229
TATAC 2028375 1.2531058 6.684351 110-119
GGAGT 3619595 1.2503223 6.9762745 210-219
CCTCA 2583195 1.2463299 6.132786 130-139
TGGGC 4371815 1.2426414 33.227184 15-19
TGATA 2246935 1.24182 6.9555335 180-189
TCACG 2872220 1.239716 9.02284 200-209
TATCA 2003180 1.2375406 5.759528 70-79
GCTCA 2848035 1.2292773 9.259105 140-149
AGAGA 2255885 1.221014 12.7464485 35-39
GTCCA 2822910 1.2184328 11.210745 70-79
AGTTC 2668885 1.2137243 5.7845087 160-169
GAACC 2408290 1.198963 8.792276 70-79
GGAAT 2545475 1.1944834 8.3841305 170-179
TTGAG 2927455 1.1909927 8.625231 200-209
GCGTA 3083170 1.1905037 9.465069 210-219
CTGAT 2612645 1.188148 5.9404254 180-189
ACAAT 1656795 1.1805941 8.957215 100-109
AGATC 2225720 1.1674899 5.772931 130-139
GCATA 2221150 1.1650926 7.782126 210-219
CTGCG 3629455 1.1531789 6.5500555 210-219
ATCAT 1861615 1.1500833 5.212507 90-99
ACTGG 2978035 1.1499081 7.193677 160-169
TGCCC 3231590 1.1477382 9.151625 60-69
CTGAA 2123475 1.1138576 13.062469 35-39
TATGT 2324240 1.1136706 7.6294103 220-229
GTCTA 2407875 1.0950253 7.763306 180-189
TAGAT 1962625 1.0846895 8.078213 240-247
CGGCT 3388270 1.0765477 7.560405 130-139
CCCAA 1932695 1.0755523 9.306304 240-247
GCGGT 3781645 1.0748919 7.049273 130-139
ATAAC 1502385 1.0705651 7.338264 80-89
CTCGG 3361810 1.0681409 7.3829465 130-139
AAAAG 1438775 1.0579028 13.179565 70-79
CCAAT 1796990 1.0536575 10.7311 240-247
AGGTT 2583725 1.0511512 17.25502 10-14
GAAGA 1927235 1.0431298 17.911297 35-39
TGGTC 3104505 1.0392818 8.0290365 50-59
TCTAC 2043500 1.0388099 7.3340163 180-189
TATAA 1382800 1.0381899 7.2626433 240-247
GTGGT 3411435 1.0216609 7.0517206 50-59
TCTGT 2557135 1.0082109 5.505791 210-219
CATCA 1716375 1.0063893 5.9321656 230-239
CGGAG 3040160 0.99671936 6.596597 210-219
AGAGC 2218680 0.9881443 6.319354 140-149
GTCAG 2551395 0.98516965 5.252743 130-139
TGTAA 1776800 0.98198915 6.6149387 230-239
GAGTT 2402895 0.9775831 5.1882377 160-169
CGGTC 3070770 0.9756692 7.1255875 130-139
CCATT 1915105 0.9735405 5.0747805 45-49
CGATG 2520315 0.97316873 22.673002 15-19
TACTG 2125945 0.9668124 5.0964475 180-189
TAGAC 1833665 0.9618393 5.310803 110-119
ACTAA 1342770 0.95682704 10.989772 240-247
AGCTT 2078740 0.94534504 5.9936137 150-159
ACCCA 1688945 0.93990463 11.526186 240-247
AAAAT 1085130 0.939705 5.520317 5
AAGAG 1727465 0.93500286 12.562262 35-39
ATATC 1509205 0.9323687 5.568365 70-79
GCTTC 2462175 0.9213637 5.576587 150-159
ACCGT 2105580 0.9088168 12.640119 45-49
TCCAG 2077195 0.8965651 5.4550343 240-247
CTTCC 2135415 0.8932349 5.250781 230-239
TGAAG 1900960 0.8920399 9.241724 35-39
CTACC 1841060 0.8882675 6.5843716 230-239
ATCAA 1240425 0.8838984 13.038514 70-79
TGCAT 1935995 0.88042915 6.400051 210-219
CAGGA 1966105 0.8756537 5.002505 60-69
CCAGC 2123285 0.86981565 5.6671786 80-89
TGCGT 2532025 0.8476351 6.970765 210-219
AGCTG 2189380 0.8453849 7.669263 30-34
GAGCT 2188660 0.84510684 5.3196096 140-149
CACAA 1245375 0.84226036 5.319602 100-109
AGCCA 1673495 0.8331466 7.713529 80-89
AATCC 1386935 0.8132235 9.973605 240-247
CCTTT 1832690 0.8077149 5.889963 50-59
TGAAC 1534245 0.8047802 5.3098207 190-199
TACAA 1101700 0.7850461 5.121501 100-109
ACGAT 1492515 0.7828909 9.064261 10-14
TGAGA 1656790 0.7774613 6.309235 240-247
TAACC 1318615 0.77316433 6.3297334 80-89
GGAAG 1940395 0.7731158 10.080331 30-34
CCCAT 1595280 0.7696845 5.1475477 240-247
AGGAT 1624465 0.7622925 9.2859535 10-14
AGACC 1516785 0.7551288 5.4337463 240-247
CTAAT 1217085 0.75190043 8.836384 240-247
AAAGG 1360120 0.73617476 7.2171125 10-14
CGGCG 2683860 0.7240329 5.1121483 130-139
TCGAA 1375465 0.7214929 12.353805 70-79
CAATC 1227075 0.7194903 9.970257 240-247
ACGTC 1664025 0.7182314 7.435635 70-79
ATACC 1223585 0.7174439 6.0544367 240-247
GCGGC 2640090 0.7122249 5.2605243 130-139
TAGGT 1746075 0.71036536 5.6339993 10-14
GAAGT 1512555 0.7097779 5.0428786 70-79
TGGAT 1725015 0.7017975 7.7226014 10-14
AATGG 1477315 0.69324124 5.546404 10-14
GTCGA 1768000 0.6826775 8.416617 70-79
GGTCC 2147330 0.68226665 9.045617 50-59
GACCG 1787830 0.6552 13.006899 45-49
AAAGC 1082105 0.6547031 9.1664095 70-79
CGAAC 1309435 0.65189993 8.6733885 70-79
CATCC 1341675 0.64732623 7.8852854 240-247
AAGGT 1338420 0.6280637 6.461476 10-14
CTTTG 1584290 0.62464374 5.1062636 50-59
GAAGC 1340845 0.59717864 9.422831 30-34
ATAGG 1271990 0.5968909 5.3246174 10-14
TAAAA 687500 0.59536386 5.158925 2
AAGCT 1099790 0.57688904 15.76704 30-34
CGTCC 1597790 0.5674744 5.8815317 70-79
TTGCC 1485255 0.55579317 6.1264687 60-69
GACGA 1209410 0.5386408 6.7044215 40-44
TCAAG 1026235 0.5383062 5.9131317 70-79
GGGAT 1421590 0.49106207 6.2375774 10-14
TAAAG 740035 0.47175092 5.029369 9
CGGTT 1401740 0.4692544 9.507827 10-14
AAGCA 768215 0.464791 5.820306 70-79
TCGAT 949245 0.4316865 6.7118306 10-14
GCGAT 843480 0.32569277 5.3263774 10-14