FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_10_SL254620.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_10_SL254620.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10761616
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12350185 8.297859 57.9715 110-119
CACAG 16265035 7.1803865 40.8112 110-119
TCCCT 17419890 6.4334073 93.10386 30-34
CAGTA 13759315 6.4281297 41.357662 60-69
ATACA 10073725 6.3956943 31.7325 110-119
GGAGA 17365170 6.1860995 93.30378 35-39
AGTAA 10297185 5.872711 43.38181 110-119
GGTGA 18891530 5.8340173 93.250885 45-49
GTAAT 11089900 5.4829006 37.040592 110-119
CCCTG 17471550 5.4771504 55.942436 30-34
AGACG 12765780 5.0624723 100.398346 40-44
GTAGT 13777760 5.012468 25.334278 70-79
ACAGT 10701980 4.9997916 33.837948 110-119
GGCCC 18307300 4.871638 56.75993 60-69
GTGAC 14169105 4.871026 83.40453 45-49
CCCCA 11888885 4.7860703 100.52378 60-69
GAGAC 12065465 4.7847514 130.42699 40-44
AATAC 6997965 4.4429283 31.635735 110-119
GTGTC 14840375 4.422681 25.089033 120-129
ACCAG 9819045 4.33473 95.54139 45-49
CGTGT 14421840 4.2979503 24.07125 120-129
TAGTA 8641925 4.27261 29.676975 80-89
ACTCC 9945340 4.2369423 92.43578 30-34
GCACA 9589195 4.23326 33.86077 110-119
TGACC 10980695 4.202283 152.08853 45-49
CCCTT 11310730 4.1772103 62.63495 20-24
GACCA 9081545 4.009152 107.35028 45-49
AGTAG 9526855 3.998157 27.51648 70-79
TGGTG 14844845 3.974101 28.172026 25-29
CTCAG 10356320 3.9633365 30.143358 140-149
CCCAG 10893770 3.9394782 83.62839 60-69
CCACT 9153330 3.8995283 28.060719 240-247
CACTC 9056130 3.858119 79.31686 25-29
TGAGG 12452530 3.845547 69.90107 35-39
CTCCC 10634035 3.7110662 57.1634 30-34
GACGG 12681090 3.7005146 112.47049 40-44
CTTGG 12296030 3.6644235 36.774506 20-24
AGGAG 10211150 3.6375797 92.808334 35-39
CAGGG 12433085 3.6281438 33.594887 50-59
CTGAG 10488510 3.6057184 97.59556 35-39
ATGGT 9843750 3.5812414 25.387226 190-199
ACGGT 10405260 3.5770993 94.13609 40-44
TGTCT 10152710 3.5644715 20.993097 180-189
TCTTG 10125340 3.554862 19.468542 170-179
GCCCC 11989480 3.5516367 73.69285 60-69
AGATA 6180280 3.5247495 18.535122 150-159
AGGCT 9917105 3.409282 43.853806 30-34
GAGGA 9549110 3.4017372 129.32214 35-39
TGTCC 10005525 3.3193882 19.155478 120-129
GCCCT 10538260 3.3036354 33.099358 20-24
CTTCA 7306535 3.2941198 16.78649 200-209
ATGCA 6993325 3.2671683 78.646965 25-29
CCAGG 9991450 3.245724 38.731274 50-59
TCAGG 9406355 3.2336974 20.411755 140-149
CCGTG 11363970 3.2001903 24.150408 120-129
CTCTC 8641305 3.191354 13.793208 100-109
GGGTT 11896630 3.1848366 42.515976 50-59
GATGG 10249445 3.1651978 32.881542 15-19
TTCCC 8529895 3.1502087 54.719494 50-59
ACAGA 5825290 3.139371 14.54981 140-149
CCTGG 11140705 3.137317 31.309288 50-59
TCGCA 7978955 3.053525 24.55145 100-109
GGATG 9794775 3.0247884 57.083847 20-24
TACAC 5794255 3.013442 22.594494 110-119
GACTC 7846050 3.0026627 21.285885 190-199
TGCAC 7827820 2.995686 101.82589 25-29
CGCAC 8266780 2.9894881 24.959564 100-109
TGGAG 9620280 2.9709015 31.25067 25-29
GGTTC 9930965 2.9595942 47.828705 50-59
TCTCA 6543500 2.9501088 20.69362 130-139
TCTCT 7415530 2.89823 15.626044 180-189
CCAGT 7534510 2.8834374 40.45637 60-69
TGGCC 10114980 2.8484643 38.633976 60-69
CAGAT 6071850 2.8366702 15.565863 150-159
TTCTT 6852765 2.834337 21.657013 170-179
CCTGA 7314565 2.7992647 66.32339 30-34
GCCGT 9914975 2.7921412 22.873888 120-129
TCACA 5314500 2.7639337 12.2711935 130-139
AGGGT 8927990 2.7571108 50.435345 50-59
CGGAT 8013060 2.7547133 64.4719 20-24
GCACT 7182515 2.7487295 72.14712 25-29
GTTCC 8271530 2.7441258 54.419285 50-59
CGGTG 10795940 2.731044 68.165535 40-44
GGTTG 10196640 2.729734 58.32904 15-19
ACCAC 5508035 2.7068675 15.824839 230-239
AGGGA 7524925 2.6806495 9.838236 150-159
AATAA 3442760 2.668312 12.973944 110-119
TCAAA 4173015 2.6493995 54.973007 70-79
CGGCC 9918270 2.639287 11.055979 120-129
GGCTG 10157585 2.5695596 24.316889 30-34
AGTCA 5458480 2.5501134 29.057888 70-79
AGATG 6066805 2.54607 17.030252 180-189
GTTGG 9319175 2.4948287 45.485405 15-19
TGTTC 7031195 2.468552 12.803117 140-149
GATGC 7145990 2.4566338 74.54799 25-29
GAGAT 5796525 2.432641 16.572636 180-189
CTGGC 8601640 2.4222949 34.26666 60-69
ACTCT 5325845 2.4011345 13.232829 200-209
ACAGC 5342185 2.3583686 12.422445 120-129
CACTA 4510360 2.3457217 15.295326 240-247
GTCTC 7070255 2.345596 12.604363 180-189
CAGAG 5908215 2.3429966 9.395462 140-149
GTTCT 6667095 2.3407218 18.354914 160-169
TGTAG 6418345 2.3350494 8.877659 210-219
TTGTC 6650385 2.3348553 14.918587 170-179
TCATT 4887050 2.331684 16.490849 150-159
TCCAT 5154440 2.3238573 14.08968 70-79
AAAAA 2593250 2.318524 9.238151 230-239
CATTT 4850125 2.314067 17.58913 150-159
TTCAT 4825325 2.3022344 17.099432 140-149
CTGGA 6674685 2.2946095 15.1366205 180-189
TCCTC 6200940 2.2900932 14.942426 130-139
GGCGG 10306440 2.2131174 36.24605 20-24
GTGAA 5258380 2.2067964 17.169477 190-199
ATATA 3270005 2.1970553 10.522258 110-119
TTGGT 6921455 2.1828964 40.56924 25-29
TTCAC 4837990 2.1811872 17.363277 200-209
GCGGA 7385305 2.1551325 77.47453 20-24
TAGTC 5293060 2.143667 24.623598 70-79
AATAT 3172860 2.1317854 12.464311 110-119
ATTTG 4956495 2.1243212 15.429288 150-159
TGAAT 4291855 2.1219141 14.706182 190-199
TTGGA 5822510 2.1182795 16.88535 170-179
GATAC 4514455 2.1090803 13.455936 150-159
GAATC 4487425 2.0964522 15.208194 190-199
CTCTG 6316225 2.0954428 14.981848 180-189
GAGTC 6088130 2.092965 12.832531 210-219
ACTAC 4014355 2.087762 12.125235 230-239
CAGCC 5765645 2.0850112 11.285238 120-129
TCTGC 6274650 2.0816498 10.626868 210-219
ATATG 4187230 2.0701873 9.915327 180-189
GATAT 4183760 2.0684714 9.744641 180-189
CTCGC 6542460 2.0509934 16.833204 100-109
GTTCA 5059570 2.0491045 15.41186 140-149
TCTCG 6168310 2.0463712 16.617218 100-109
CTACT 4512985 2.0346599 8.1115265 230-239
TCGGC 7210190 2.0304506 8.555952 190-199
AATAG 3538130 2.0178733 8.496692 240-247
CCTTC 5431695 2.0060005 13.638018 200-209
ATAGA 3500370 1.9963381 10.140654 240-247
AATCG 4272875 1.996218 13.737074 190-199
ACACA 3327360 1.9961921 13.761485 110-119
GTCAA 4267400 1.99366 39.17558 70-79
CCTTG 5995845 1.9891549 41.440445 20-24
CACGG 6122810 1.9889957 7.571267 200-209
TTGGG 7398280 1.9805874 72.03627 15-19
TACAG 4214250 1.9688294 11.937533 160-169
TCCCA 4594145 1.957211 12.049735 240-247
GTTGT 6167180 1.9450123 7.765002 210-219
TTTGC 5492580 1.9283664 11.7516775 150-159
TTGCA 4760075 1.9278102 13.451683 150-159
GGTCA 5534305 1.902572 9.793424 130-139
GCTGA 5521220 1.8980737 38.40266 30-34
TACCA 3644060 1.8951813 11.967031 230-239
GAACT 4054245 1.8940777 11.444236 160-169
GTCAC 4922575 1.8838565 9.43723 140-149
GTCCT 5675985 1.8830396 12.887946 130-139
TGGAA 4467370 1.8748314 9.224016 25-29
AGTAT 3759845 1.8588859 12.008516 220-229
GCTGT 6234045 1.8578501 9.806859 140-149
ATTGT 4330285 1.8559315 8.350842 170-179
ATGTA 3745095 1.8515934 12.041132 70-79
TACTA 3334380 1.8351659 10.51976 230-239
CAGGC 5610385 1.8225343 14.043781 140-149
AGTCT 4494650 1.8203143 13.139906 210-219
CATAG 3833800 1.7910893 8.139049 210-219
CTGTT 5095600 1.7889924 12.541367 140-149
TCAGC 4667030 1.7860601 11.618255 140-149
TTGTA 4134660 1.772088 10.190705 210-219
GTCTG 5920505 1.7644099 8.901704 210-219
AGAAC 3260530 1.7571679 11.203745 160-169
TGGGG 7697650 1.7492381 43.23658 15-19
CCAGA 3907800 1.7251433 24.758621 60-69
CAGCT 4500395 1.7222896 9.469674 150-159
GACGT 4996480 1.7176796 10.281473 170-179
ATAGT 3462215 1.7117363 7.1599355 220-229
CACCA 3479190 1.7098122 11.289452 230-239
GCAGA 4289045 1.7008888 13.662893 150-159
TTCAG 4191300 1.6974587 10.271685 150-159
TCTGG 5675685 1.6914494 12.234064 180-189
GTGGA 5451315 1.6834563 38.583637 25-29
ACGGC 5173425 1.6805879 12.39456 120-129
CCCAC 4173010 1.6799154 11.0358095 240-247
ATCTC 3713185 1.6740736 7.152745 100-109
TGACT 4112130 1.6653951 12.240516 190-199
GAGAA 3421560 1.6564249 11.009735 160-169
TGCAG 4782255 1.6440338 12.583739 150-159
GGACG 5609595 1.6369561 8.112095 170-179
CTCCA 3838935 1.6354743 8.512864 240-247
GGAGG 6226395 1.6321675 26.66515 30-34
ATAAT 2413590 1.621646 5.7341323 110-119
GTCCC 5165165 1.6192257 18.079943 50-59
GGGGC 7524605 1.6157697 39.96743 15-19
AACTG 3457595 1.6153324 10.79068 160-169
CGACT 4169260 1.5955648 9.7082 190-199
TGGTT 5018980 1.5828917 16.610403 10-14
ATAAA 2038625 1.5800369 6.5456996 110-119
AACCA 2630340 1.578027 12.145255 70-79
TCGAC 4101530 1.5696447 10.161649 190-199
CACTG 4100400 1.5692124 6.8523307 230-239
CTCTT 4002155 1.5641721 9.24429 200-209
CAAAA 2132705 1.5619447 20.640299 70-79
ACGGA 3935670 1.5607525 10.10141 200-209
AGCTC 4069860 1.5575247 9.603685 140-149
GGGCG 7210685 1.5483613 46.803654 20-24
CAAAG 2852030 1.5370188 27.62985 70-79
ACTGA 3286540 1.5354183 7.5534716 180-189
ATGGG 4923995 1.520611 53.293728 15-19
CCTCG 4782165 1.4991592 9.199534 130-139
CTGGT 5015930 1.4948313 7.128884 160-169
ACACG 3360660 1.4836018 9.504428 120-129
TCAGA 3160575 1.4765693 9.0220585 130-139
GCTCT 4435440 1.4714819 9.235355 130-139
TGGAC 4266790 1.4668283 11.241979 170-179
AAATA 1887910 1.463225 5.503172 6
CTCGA 3822985 1.4630463 9.329831 190-199
AGCCG 4501120 1.4621892 10.854742 120-129
TCCAC 3407535 1.4516879 10.45975 240-247
GAGGG 5529525 1.4494922 9.529973 200-209
ATCGG 4216335 1.449483 9.483666 190-199
CCACC 3594110 1.4468694 8.318934 230-239
AATTG 2908960 1.4382042 9.302742 170-179
GGCCG 6008780 1.4363476 11.891865 120-129
CCATC 3323020 1.4156827 10.850917 240-247
CAGAC 3201510 1.4133433 13.2396345 60-69
CGTAG 4102255 1.4102648 8.3846655 210-219
CTTGA 3463530 1.402715 9.603604 200-209
GGGAG 5320035 1.3945771 6.9910307 150-159
TAGTT 3240465 1.3888419 8.560546 220-229
TATGG 3796715 1.3812777 7.104193 180-189
CAATA 2174755 1.3807273 7.7825127 100-109
CATGT 3405275 1.379122 11.510198 70-79
GAATT 2732600 1.3510109 10.42021 170-179
ACTAT 2449415 1.3481015 7.2516522 230-239
ACCAT 2579555 1.3415599 10.870311 45-49
ACGTG 3851305 1.3239939 9.70298 170-179
CCATG 3444210 1.3180902 11.545805 70-79
ACTCG 3442815 1.3175563 9.489207 190-199
GGCTC 4677645 1.3172646 8.338434 130-139
TATAC 2390670 1.3157697 6.533675 110-119
GTGCT 4394355 1.3095915 7.4337416 220-229
GAGGC 4440685 1.2958523 23.461683 30-34
GTCCA 3381545 1.2941084 12.973791 70-79
TGATA 2611430 1.2911037 7.057538 180-189
ACTGT 3154220 1.2774458 5.394713 230-239
AGAGT 3037045 1.2745637 5.0002055 210-219
GCTCA 3317760 1.269698 9.463412 140-149
ACTTC 2814830 1.269054 5.670235 210-219
AAGTA 2224850 1.2688808 16.871857 70-79
GTGTT 3986675 1.2573222 7.3953233 160-169
TTCCA 2783065 1.2547328 7.348678 230-239
ATCCA 2412270 1.2545593 16.351767 240-247
GGTGG 5489060 1.2473513 38.55649 25-29
GGGCC 5187360 1.2399942 29.692657 15-19
GGAGT 4012125 1.2390105 7.022481 210-219
AAAGT 2170995 1.2381662 20.599806 70-79
TGTGC 4151045 1.2370812 6.347959 220-229
TTGAG 3379995 1.2296714 8.721807 200-209
CCTCA 2870460 1.2228818 5.8601604 130-139
AGAGA 2522480 1.2211678 13.163465 35-39
TATCA 2210310 1.2165036 5.947327 70-79
GAACC 2754935 1.2161976 9.039145 70-79
GGAAT 2885075 1.2107861 8.374113 170-179
CTGAT 2972675 1.2039207 6.178381 180-189
GCGTA 3500295 1.2033242 9.194048 210-219
TGGGC 4743845 1.2000483 31.821081 15-19
ACTGG 3445965 1.1846468 7.1810584 160-169
TGCCC 3774660 1.1833168 10.579239 60-69
AGTTC 2906670 1.1771891 5.673346 160-169
CTGCG 4152910 1.1694947 6.356219 210-219
GTCTA 2849235 1.153928 7.734532 180-189
ACAAT 1808935 1.1484723 8.781111 100-109
TCACG 2987800 1.1434231 8.695957 200-209
CTGAA 2435870 1.1379989 13.984275 35-39
ATCAT 2029590 1.1170396 5.0389185 90-99
GCATA 2390095 1.1166136 7.5717726 210-219
TATGT 2595725 1.1125106 7.3612576 220-229
TAGAT 2234575 1.1047848 8.213136 240-247
CCCAA 2243385 1.1024886 9.9825325 240-247
TCTAC 2434815 1.0977259 7.3113675 180-189
AGCTT 2710350 1.0976802 6.038272 150-159
GCGGT 4320060 1.0928437 7.090242 130-139
GAAGA 2246020 1.0873296 18.527956 35-39
AGAGC 2727135 1.0814886 6.434186 140-149
TGGTC 3625415 1.0804346 9.4089775 50-59
TTGGC 3618370 1.078335 5.026308 170-179
AGATC 2303405 1.0761135 5.412024 130-139
CTCGG 3794695 1.0686183 7.636793 130-139
AAAAG 1620765 1.0662951 13.521527 70-79
AGGTT 2926310 1.0646168 18.28077 10-14
CGGCT 3770065 1.0616823 7.48427 130-139
GTGGT 3958700 1.0597802 8.306729 50-59
CCAAT 2032760 1.057186 11.187494 240-247
TATAA 1542110 1.0361149 7.552569 240-247
ATAAC 1625675 1.0321226 7.037299 80-89
CGGTC 3587435 1.0102521 7.1953287 130-139
AAGAG 2085140 1.0094453 13.011398 35-39
CATCA 1928155 1.0027835 5.7504153 230-239
TACTG 2471110 1.0007892 5.2527847 180-189
ACCGT 2589065 0.99082845 14.689649 45-49
TCTGT 2805960 0.9851325 5.4386945 210-219
ATATC 1786480 0.9832375 5.872643 70-79
CGGAG 3333105 0.9726454 6.5313735 210-219
CCATT 2149800 0.9692282 5.130901 50-59
GCTTC 2920830 0.9690015 5.5015783 150-159
TGTAA 1954815 0.9664702 6.56785 230-239
CGATG 2798520 0.96206945 22.354403 15-19
GAGTT 2635160 0.958694 5.240636 160-169
GTCAG 2758045 0.94815505 5.0118895 130-139
ACCCA 1913060 0.9401537 11.712806 240-247
ACTAA 1465125 0.9301912 11.468136 240-247
TAGAC 1985230 0.9274673 5.0343146 110-119
TGAAG 2173355 0.9120968 9.845422 35-39
TCCAG 2377075 0.90970033 5.5258746 240-247
CTACC 2119015 0.9027488 6.872093 230-239
ATCAA 1414350 0.89795476 13.318564 70-79
CTTCC 2402245 0.8871825 5.150443 230-239
CCAGC 2447785 0.8851844 5.7031846 80-89
GAGCT 2566145 0.8821841 5.490384 140-149
CCTTT 2196900 0.85861987 6.948772 50-59
AGCTG 2482470 0.85341847 7.3072696 30-34
TGCGT 2849955 0.8493344 6.7871294 210-219
TGCAT 2093670 0.84792745 6.181569 210-219
AATCC 1619545 0.8422834 10.572928 240-247
TGAAC 1756645 0.820676 5.28487 190-199
CACAA 1355140 0.8129929 5.0265746 100-109
ACGTC 2117555 0.81038284 8.443314 70-79
AGCCA 1827245 0.8066582 7.442831 80-89
GTATG 2191485 0.79728115 5.031941 220-229
GGAAG 2192065 0.78089255 10.140525 30-34
AGACC 1730475 0.763938 5.3044686 240-247
TAACC 1453725 0.7560448 6.406213 80-89
TAGGT 2074575 0.7547483 6.1489873 10-14
CAATC 1432265 0.74488395 10.534794 240-247
AAAGG 1530320 0.74084926 7.2269807 10-14
CTAAT 1344125 0.73977536 9.02851 240-247
AGGAT 1760920 0.7390093 8.546469 10-14
CGGCG 3085280 0.7375099 5.021651 130-139
TCGAA 1576245 0.7363961 12.810135 70-79
GGTCC 2605875 0.73383653 10.28697 50-59
TGAGA 1736210 0.72863925 5.9589615 240-247
GACCG 2240845 0.72793865 14.680052 45-49
AAATG 1275155 0.7272489 5.341617 9
GCGGC 3042290 0.72723347 5.2209454 130-139
ATACC 1383775 0.71966565 5.894607 240-247
GTCGA 1985555 0.68259 8.664469 70-79
CTTTG 1944020 0.6825176 6.079492 50-59
ACGAT 1453245 0.6789326 8.845623 10-14
CGAAC 1521565 0.6717122 8.9061985 70-79
TGGAT 1841625 0.66999906 6.845384 10-14
CGTGG 2639190 0.6676347 5.517948 50-59
AATGG 1551485 0.6511153 5.5838265 10-14
AAAGC 1204610 0.6491896 9.2279825 70-79
CGTCC 2016655 0.6322004 6.718456 70-79
AAGGT 1497895 0.62862504 6.519905 10-14
TTGCC 1851360 0.6141989 7.0189176 60-69
CATCC 1391115 0.59264684 7.1535144 240-247
GAAGC 1488325 0.5902189 9.3289585 30-34
ATAGG 1351775 0.5673026 5.4235134 10-14
AAGCT 1187450 0.5547574 15.093097 30-34
TCAAG 1134685 0.53010637 5.81648 70-79
GACGA 1327315 0.5263678 6.450957 40-44
AAGCA 901425 0.4857968 5.970394 70-79
CGGTT 1585050 0.47237152 9.659608 10-14
GGGAT 1509235 0.4660767 5.985436 10-14
TAAAG 793315 0.452445 5.319789 9
TCGAT 1069185 0.43301538 6.83562 10-14
GCGAT 930020 0.31972036 5.1872697 10-14