FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_1_SL254619.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_1_SL254619.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2205065
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2458840 7.94766 56.142323 110-119
CACAG 3225465 6.8204727 38.685825 110-119
TCCCT 3488975 6.3153653 89.27187 30-34
CAGTA 2783760 6.2816896 40.98603 60-69
GGAGA 3478775 6.009486 90.28265 35-39
ATACA 1965045 5.951945 30.013285 110-119
GGTGA 3783780 5.749411 90.69319 45-49
AGTAA 2052580 5.619284 40.798428 110-119
CCCTG 3540945 5.428647 53.307156 30-34
GTAAT 2217080 5.338862 35.63778 110-119
ACAGT 2137195 4.822685 32.2479 110-119
GTAGT 2684495 4.8160195 24.468588 70-79
GGCCC 3708820 4.815928 55.77149 60-69
AGACG 2518950 4.8143334 95.53528 40-44
GTGAC 2827505 4.7534223 80.4803 45-49
GAGAC 2476360 4.732933 124.78734 40-44
CCCCA 2409065 4.6455812 95.89807 60-69
GTGTC 2955075 4.369764 25.207306 120-129
ACTCC 2091575 4.304152 89.7797 30-34
ACCAG 2012805 4.256218 92.71045 45-49
GCACA 1994085 4.2166333 31.763336 110-119
CGTGT 2829570 4.1841755 24.181757 120-129
AATAC 1376935 4.1706123 29.956053 110-119
CCCTT 2298865 4.1611567 64.361694 20-24
TGACC 2197225 4.0867977 146.60228 45-49
TGGTG 3001485 4.0116224 29.511744 25-29
TAGTA 1647035 3.9661593 26.522438 80-89
CTCAG 2102020 3.909718 28.79157 140-149
CCCAG 2228450 3.8840861 80.19019 60-69
GACCA 1836040 3.8824358 103.402145 45-49
CCACT 1870610 3.8494382 26.57696 240-247
CACTC 1866310 3.84059 76.91102 25-29
AGTAG 1876790 3.8278491 26.890017 70-79
TGAGG 2497430 3.7948167 68.08305 35-39
AAAAA 880020 3.67645 18.350708 220-229
CTTGG 2468490 3.6502354 38.44429 20-24
GACGG 2531405 3.6044292 108.967255 40-44
AGGAG 2085380 3.6024356 89.64148 35-39
CAGGG 2522435 3.591657 33.142223 50-59
CTGAG 2125680 3.5735586 95.01949 35-39
CTCCC 2096420 3.5559556 54.670483 30-34
ATGGT 1957550 3.5118706 24.433756 190-199
ACGGT 2071190 3.4819536 91.31771 40-44
GCCCC 2423125 3.4811735 71.290245 60-69
TGTCT 1987215 3.469465 20.546139 170-179
TCTTG 1977560 3.4526086 18.92412 170-179
AGGCT 2040265 3.4299643 44.089672 30-34
AGATA 1217140 3.3321261 18.357054 150-159
GCCCT 2156570 3.3062522 33.94468 20-24
GAGGA 1912175 3.3032289 124.66058 35-39
TGTCC 2011985 3.2916968 19.211649 120-129
CTTCA 1495545 3.2842505 16.489223 200-209
CCTGG 2331600 3.2308805 31.166973 50-59
CCAGG 2035740 3.207028 38.173386 45-49
TTCCC 1767490 3.1993194 54.01402 50-59
TCAGG 1900750 3.1954205 19.529509 140-149
GATGG 2098840 3.1891637 33.878056 15-19
ATGCA 1408950 3.1793642 76.122856 25-29
GGGTT 2373205 3.1718974 42.85186 50-59
CTCTC 1722120 3.1171956 13.429155 100-109
CCGTG 2237470 3.1004455 24.109941 120-129
GGATG 2002040 3.0420773 56.468803 20-24
CCAGT 1633320 3.0379448 40.33695 60-69
TGGAG 1987690 3.0202725 32.366253 25-29
TGCAC 1604275 2.9839215 99.25809 25-29
ACAGA 1151820 2.9549007 13.728286 140-149
GGTTC 1981185 2.9296415 47.86959 50-59
TCGCA 1574430 2.9284103 23.77372 100-109
TCTCA 1332450 2.92609 20.784533 130-139
TCACA 1149975 2.8710363 11.7394905 130-139
GACTC 1540740 2.8657477 20.318708 190-199
TCTCT 1478115 2.8551664 14.98961 180-189
CGCAC 1635115 2.8499308 24.116293 100-109
TACAC 1138050 2.8412642 21.981966 110-119
TGGCC 2040140 2.8270068 37.98961 60-69
CAGAT 1234640 2.786025 15.165253 150-159
GCCGT 2006335 2.7801633 22.502302 120-129
AGGGT 1823880 2.7713652 50.18634 50-59
TTCTT 1344270 2.770973 20.976479 160-169
GTTCC 1680875 2.7499862 54.199615 50-59
GGTTG 2028750 2.7115176 58.636665 15-19
CGGAT 1610965 2.708252 63.270306 20-24
GCACT 1450535 2.697968 70.34844 25-29
CGGTG 2151910 2.6951652 66.895676 40-44
CCTGA 1447485 2.6922946 62.773533 30-34
AGTCA 1177770 2.6576953 28.503248 70-79
GGCTG 2102360 2.6331062 24.852182 30-34
ACCAC 1122735 2.6266665 14.823665 230-239
AGGGA 1511365 2.6108408 9.596902 150-159
AATAA 703625 2.5856144 12.33625 110-119
CGGCC 1969310 2.5571625 10.595131 120-129
TCAAA 837995 2.5382116 52.041267 70-79
AGATG 1225620 2.499741 16.92289 180-189
GTTGG 1866160 2.4942088 45.587337 15-19
CTGGC 1765310 2.446177 33.995667 60-69
TGTTC 1394085 2.4339232 12.927069 140-149
GATGC 1443595 2.4268806 73.32272 25-29
GAGAT 1180045 2.4067874 16.31248 180-189
GTCTC 1448865 2.3704076 12.165806 180-189
ACTCT 1062150 2.332505 12.852528 200-209
CAGAG 1211970 2.3163726 9.000277 140-149
TCCAT 1053955 2.3145087 12.551147 70-79
CTGGA 1369530 2.3023672 14.907774 180-189
TTGTC 1315740 2.297141 15.289892 170-179
TCCTC 1264675 2.2891781 14.400612 130-139
TCATT 971745 2.277255 15.958518 140-149
TTCAT 971710 2.277173 17.263115 140-149
GTTCT 1301415 2.2721314 18.163 160-169
TGTAG 1265635 2.270566 8.403967 210-219
CATTT 968715 2.2701542 16.782158 150-159
TTGGT 1432865 2.2610874 42.61001 25-29
GGCGG 2086085 2.212917 35.968315 20-24
ACAGC 1038715 2.196436 11.697568 110-119
GCGGA 1517415 2.1606243 75.92704 20-24
TTCAC 982460 2.157504 17.108221 200-209
ATATA 666985 2.1558824 10.30212 110-119
GTGAA 1049905 2.1413574 16.196987 190-199
TAGTC 1077045 2.13779 24.552202 70-79
CACTA 848320 2.1179223 13.784487 240-247
ATTTG 991065 2.0992103 14.931985 150-159
TGAAT 871525 2.0986845 14.244671 190-199
CTCTG 1277065 2.089335 14.498412 180-189
GAGTC 1241940 2.0878708 12.155117 210-219
TCTCG 1266165 2.0715022 16.336863 100-109
TCTGC 1265955 2.0711586 11.098762 210-219
AATAT 640015 2.0687077 12.226583 110-119
GAATC 916070 2.0671568 14.863386 190-199
TTGGA 1141580 2.0480096 16.332563 170-179
CCTTG 1247065 2.0402534 42.992264 20-24
GATAT 844280 2.033077 9.393358 180-189
CAGCC 1164355 2.0294175 10.725232 120-129
GTTCA 1019940 2.0244443 15.423408 140-149
CCTTC 1113940 2.0163336 13.181567 200-209
ACTAC 802020 2.0023293 12.21255 230-239
TCGGC 1438620 1.9934851 8.749308 190-199
GATAC 882820 1.9921262 13.133166 150-159
TTGGG 1488105 1.9889209 72.17646 15-19
ATATG 824745 1.9860355 9.37581 180-189
CTCGC 1294540 1.9846684 16.294607 100-109
CTACT 899400 1.9751024 8.2837715 230-239
GTCAA 872685 1.9692562 38.479485 70-79
AATAG 715010 1.9574603 8.16269 240-247
ATAGA 712080 1.9494392 9.389948 240-247
GAACT 858725 1.9377549 11.024154 160-169
GCTGA 1148290 1.9304324 38.27883 30-34
AATCG 853120 1.9251068 13.453783 190-199
GTCAC 1031645 1.9188404 9.105017 130-139
TCCCA 931645 1.9171878 11.215865 240-247
CACGG 1215955 1.9155695 7.4473195 200-209
GTTGT 1204615 1.9009048 7.4233365 210-219
TTGCA 952260 1.8901085 13.112626 150-159
ACACA 663070 1.8820157 13.2374735 110-119
GCTGT 1272230 1.8812872 9.724817 140-149
TTTGC 1070940 1.8697466 11.521606 150-159
TACAG 827745 1.8678468 11.015233 160-169
GGTCA 1106175 1.8596314 9.574579 130-139
TGGAA 911260 1.858581 9.182274 25-29
GTCCT 1130785 1.8500142 12.239605 130-139
TACCA 736700 1.8392508 11.400127 230-239
CTCCA 887665 1.8266835 8.518556 240-247
AGTCT 919275 1.8246375 12.430277 210-219
GTCTG 1229555 1.8181825 8.628445 210-219
TCAGC 974595 1.8127285 11.272689 140-149
ATTGT 852830 1.8064097 8.386126 170-179
AGTAT 749165 1.8040344 11.358511 220-229
ATGTA 745275 1.794667 10.643393 70-79
CAGGC 1134020 1.7864922 13.361778 140-149
CTGTT 1021770 1.7839012 12.477442 140-149
CATAG 789975 1.782617 8.0426445 210-219
TACTA 666235 1.7750081 10.753077 230-239
TGGGG 1566540 1.7733614 43.257812 15-19
GTGGA 1147775 1.7440312 39.75904 25-29
CCAGA 821805 1.7377645 22.284563 60-69
ATCTC 787440 1.7292358 6.653691 100-109
TTCAG 871090 1.728997 9.829995 150-159
TTGTA 812995 1.7220337 9.795412 210-219
CACCA 732455 1.7135968 10.445646 230-239
ATAGT 708820 1.7068812 7.044644 220-229
TCTGG 1136440 1.6804904 11.916566 180-189
GCAGA 868310 1.659554 13.325251 150-159
CCCAC 858945 1.6563683 10.304482 240-247
GGAGG 1285830 1.6548281 27.736418 30-34
CAGCT 886475 1.648827 8.898615 150-159
AGAAC 640090 1.642099 10.467867 160-169
GGGGC 1526245 1.6190393 39.64622 15-19
TGCAG 962335 1.6178167 12.468016 150-159
TGACT 814120 1.615919 11.801127 190-199
ACGGC 1024565 1.614061 11.81468 120-129
GACGT 959390 1.6128657 10.031603 170-179
ATAAA 429095 1.5767977 6.5880933 8
CACTG 845300 1.5722423 6.5441356 230-239
GTCCC 1024955 1.5713657 16.435755 50-59
ATGGG 1034080 1.571273 54.800903 15-19
ATAAT 484870 1.5672357 5.640952 100-109
TGGTT 988980 1.5606287 16.958271 10-14
GGACG 1093075 1.5564129 7.776887 170-179
GAGAA 669560 1.5525379 10.335522 160-169
GGGCG 1452345 1.5406464 46.622986 20-24
AACTG 677980 1.529895 10.078923 160-169
ACACG 720870 1.5243304 8.949776 110-119
CAAAG 593245 1.5219218 26.559439 70-79
ATCGG 903205 1.518411 9.603427 190-199
TCAGA 671320 1.5148662 9.0884285 130-139
CTCTT 784185 1.5147527 8.803446 200-209
CAAAA 439620 1.5138311 18.634598 70-79
ACTGA 670705 1.5134784 7.0698023 180-189
AACCA 530805 1.5066031 11.316098 70-79
CGACT 806460 1.5000006 9.153023 190-199
ACGGA 783490 1.497442 9.923763 200-209
AGCTC 804880 1.4970617 9.133803 140-149
TCGAC 794500 1.4777552 9.589789 190-199
CCTCG 961110 1.4734844 8.875221 130-139
TCCAC 708305 1.457587 10.652959 240-247
CTGGT 984600 1.4559594 6.8448095 160-169
CCACC 750895 1.4480072 7.627807 230-239
CCATC 699645 1.4397658 11.331812 240-247
GCTCT 877775 1.4360787 9.142979 130-139
TGGAC 847605 1.4249399 10.639108 170-179
CTTGA 716185 1.4215313 9.313077 200-209
ATCCA 567805 1.4175863 16.547396 240-247
GAGGG 1098820 1.4141513 9.162556 200-209
GGCCG 1199860 1.4082161 11.450297 120-129
AGCCG 889115 1.4006782 10.4388075 120-129
TAGTT 656160 1.3898358 8.354413 220-229
AATTG 574950 1.3845141 9.010989 170-179
CTCGA 743295 1.3825148 8.78863 190-199
CGTAG 819620 1.3778932 8.185019 210-219
GAGGC 954475 1.3590624 24.005585 30-34
CAGAC 642215 1.3580089 11.592672 60-69
CATGT 682625 1.3549191 10.184091 70-79
TATGG 746070 1.3384595 6.8979254 180-189
GGGAG 1036455 1.3338891 6.804629 150-159
CAATA 439590 1.3314787 7.609614 100-109
TCACG 711115 1.3226606 8.6988535 200-209
GGCTC 940535 1.3032923 8.381063 130-139
GAATT 540665 1.3019539 10.056704 170-179
GTGTT 824940 1.3017704 7.481385 160-169
GGTGG 1145095 1.2962755 40.169086 25-29
ACTGT 647340 1.284883 5.283361 230-239
CCATG 689455 1.2823734 10.275253 70-79
GTGCT 867050 1.2821345 7.322351 220-229
ACTAT 480435 1.2799927 6.8459473 230-239
ACCAT 511420 1.276815 9.693009 45-49
ACGTG 758580 1.2752767 9.356807 170-179
GGAAT 623660 1.2719998 8.19905 170-179
GGGCC 1081145 1.2688862 30.668076 15-19
CTGAA 562265 1.2687783 12.676275 35-39
TTCCA 575640 1.2641183 6.984458 230-239
TATAC 473555 1.2616628 6.2874436 110-119
ACTTC 572430 1.257069 5.602549 220-229
TGTGC 846375 1.2515619 6.4155955 220-229
CATCA 499690 1.2475299 5.7054596 230-239
ACTCG 668625 1.24363 8.934525 190-199
AGAGA 535365 1.2413741 12.415259 35-39
TGGGC 989230 1.2389637 33.13641 15-19
CCTCA 600460 1.2356578 5.856358 130-139
GGAGT 811810 1.2335361 6.562046 210-219
GCTCA 662425 1.2320981 9.181359 140-149
GTCCA 662330 1.2319214 11.534253 70-79
AGATC 544915 1.2296274 6.0634127 130-139
TGATA 509310 1.2264491 6.779482 180-189
AAGTA 441945 1.209899 15.838538 70-79
AGTTC 606255 1.2033348 5.8302846 160-169
TTGAG 670575 1.2030205 8.58445 200-209
AAAGT 435650 1.1926653 19.488619 70-79
CTGAT 594175 1.1793576 5.8292933 180-189
TGCCC 767445 1.1765753 9.616069 60-69
GAAGA 506195 1.1737363 17.270391 35-39
GCGTA 697830 1.1731477 9.190054 210-219
GAACC 553445 1.1702985 8.398157 70-79
TCCAG 619435 1.1521375 5.717459 240-247
TAGAT 472400 1.1375676 7.8434577 240-247
TATCA 425465 1.1335397 5.1404243 70-79
ACTGG 666825 1.121024 6.7900963 160-169
GTCTA 561860 1.1152169 7.3515244 180-189
AGAGC 582965 1.1141895 6.4924755 140-149
GCATA 492750 1.1119143 7.2627687 210-219
CTGCG 800195 1.1088243 6.3378506 210-219
ACAAT 363825 1.1019933 8.434388 100-109
AAGAG 471870 1.0941455 12.25493 35-39
TATGT 513820 1.0883405 7.073104 220-229
CCCAA 463585 1.0845686 9.324486 240-247
AGGTT 594345 1.0662628 18.499752 10-14
TGGTC 718030 1.0617739 8.55313 50-59
GCGGT 840975 1.0532813 6.9302993 130-139
GTGGT 785265 1.0495428 7.5871387 50-59
TCTGT 601115 1.0494826 5.421457 210-219
CCAAT 419345 1.0469398 10.423055 240-247
CTCGG 754440 1.045422 7.1974363 130-139
AAAAG 335130 1.0430562 12.017509 70-79
ACCCA 445485 1.0422233 11.302494 240-247
TCTAC 474145 1.041233 6.7760134 180-189
CGGCT 749110 1.0380361 7.4230027 130-139
TATAA 315515 1.019833 6.6855817 240-247
ATATC 379920 1.0121969 5.2016115 70-79
ATAAC 331540 1.0042049 6.647116 80-89
GCCAC 565930 0.9863901 5.077778 80-89
GTCAG 585355 0.9840618 5.3617063 130-139
CTTCT 507395 0.9800978 5.0939445 210-219
CGATG 582645 0.9795059 23.035353 15-19
CGGTC 701685 0.9723196 6.985047 130-139
GAGTT 540215 0.96915287 5.3835874 160-169
CGGAG 679140 0.9670172 6.0417113 210-219
TGTAA 396820 0.95556647 6.3369703 230-239
GCTTC 580815 0.9502391 5.329841 150-159
ACTAA 311610 0.94383866 11.148209 240-247
AGCTT 475105 0.9430197 5.513299 150-159
CCAGC 538010 0.93772686 5.7998238 80-89
TGAAC 413600 0.9333085 5.2908106 190-199
ACCGT 496775 0.9239922 12.997191 45-49
ACGAT 405815 0.91574126 9.243561 10-14
TAGAC 402990 0.90936655 5.134147 110-119
TGAAG 442835 0.90319407 9.231699 35-39
GGAAG 516060 0.8914792 10.066279 30-34
AATCC 352285 0.87951744 10.016077 240-247
ACGTC 467195 0.868974 7.696622 70-79
CTACC 420655 0.8656458 6.21768 230-239
TGCAT 435225 0.8638632 5.930866 210-219
AGACC 408445 0.8636857 5.1884837 240-247
TTCTG 482065 0.841634 5.326355 210-219
AGCTG 500175 0.8408626 7.079954 30-34
GAGCT 498790 0.8385342 5.193541 140-149
ATCAA 273510 0.8284372 11.738924 70-79
TGCGT 555420 0.8213173 6.8706083 210-219
CCTTT 420115 0.8115053 6.2581043 50-59
CACAA 284920 0.8086988 5.0799794 100-109
AAAGG 336250 0.7796775 7.2062035 10-14
AGCCA 368120 0.7784157 7.297246 80-89
CCCAT 376630 0.77504873 5.2656455 240-247
TGAGA 375675 0.76621646 5.7770934 240-247
AGGAT 368300 0.75117457 8.728474 10-14
CTAAT 281550 0.75011593 8.870756 240-247
TAACC 296875 0.7411804 6.282617 80-89
GACCG 470180 0.7407038 13.053061 45-49
CAATC 295415 0.7375354 10.104334 240-247
TAGGT 397805 0.71366745 6.239092 10-14
TCGAA 315695 0.7123811 12.305276 70-79
GGTCC 509135 0.7055046 9.460398 50-59
TGGAT 390585 0.70071465 7.1189356 10-14
GCGGC 592460 0.6953408 5.0199227 130-139
ATACC 276190 0.68953806 5.684988 240-247
AATGG 332435 0.6780253 5.4914336 10-14
CATCC 327540 0.6740289 7.9477077 240-247
GTCGA 396180 0.66603273 8.568611 70-79
AAGGT 324345 0.6615252 6.636259 10-14
CGAAC 305295 0.6455678 8.4890175 70-79
CGTGG 514255 0.64408004 5.0370717 50-59
CTTTG 365760 0.6385779 5.509344 50-59
AAAGC 243845 0.6255646 8.321088 70-79
TAAAA 163430 0.6005571 5.4855404 8
GAAGC 313155 0.5985163 9.201127 30-34
CGTCC 379185 0.5813312 5.98226 70-79
TTGCC 355315 0.5813111 6.5101867 60-69
ATAGG 279200 0.56944865 5.1323223 10-14
AAGCT 243320 0.54906344 14.917996 30-34
TCAAG 241290 0.5444826 5.8394613 70-79
GACGA 268800 0.5137429 6.10358 40-44
CGGTT 322935 0.4775343 9.608971 10-14
GGGAT 309600 0.47043365 6.456589 10-14
AAGCA 182815 0.46899706 5.2573414 70-79
TCGAT 216455 0.42963415 6.8444977 10-14
GCGAT 187520 0.3152467 5.0174856 10-14