FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_10_SL254620.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_10_SL254620.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11417693
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13083525 8.287774 57.786415 110-119
CACAG 17207500 7.1611094 40.532867 110-119
CAGTA 14570255 6.4167724 41.303528 60-69
TCCCT 18437220 6.4144506 92.775246 30-34
ATACA 10667805 6.3855934 31.697598 110-119
GGAGA 18395380 6.1789894 93.19164 35-39
AGTAA 10898115 5.860715 43.080357 110-119
GGTGA 19978805 5.81588 92.96564 45-49
GTAAT 11728300 5.4660244 36.7769 110-119
CCCTG 18467050 5.4544077 55.71254 30-34
AGACG 13524385 5.056536 100.225105 40-44
GTAGT 14578935 4.9990287 25.25968 70-79
ACAGT 11327550 4.988678 33.583035 110-119
GGCCC 19374965 4.858219 56.482365 60-69
GTGAC 14981880 4.854441 83.11556 45-49
GAGAC 12793890 4.7834163 130.30508 40-44
CCCCA 12597080 4.778699 100.35302 60-69
AATAC 7410610 4.4358835 31.587082 110-119
GTGTC 15704355 4.4099154 25.04998 120-129
ACCAG 10389865 4.323868 95.11255 45-49
CGTGT 15261650 4.2855997 24.00828 120-129
TAGTA 9150415 4.2645903 29.525982 80-89
ACTCC 10532440 4.228202 92.25231 30-34
GCACA 10146640 4.2226467 33.63838 110-119
TGACC 11610515 4.1874666 151.42444 45-49
CCCTT 12011415 4.178863 62.47406 20-24
GACCA 9598590 3.994569 106.837524 45-49
AGTAG 10084640 3.9900928 27.458097 70-79
TGGTG 15696560 3.9599319 28.126202 25-29
CTCAG 10969100 3.9561327 30.086401 140-149
CCCAG 11550805 3.9366343 83.50707 60-69
CCACT 9719385 3.901805 28.11007 240-247
CACTC 9598085 3.8531094 78.97479 25-29
TGAGG 13188385 3.8391716 69.87863 35-39
CTCCC 11268560 3.7046356 56.967037 30-34
GACGG 13438485 3.696658 112.212746 40-44
CTTGG 13031300 3.659299 36.72279 20-24
AGGAG 10827665 3.637002 92.74149 35-39
CAGGG 13157205 3.6192834 33.515064 50-59
CTGAG 11124570 3.604592 97.63948 35-39
ATGGT 10414520 3.5710762 25.251318 190-199
ACGGT 11018420 3.5701969 93.943794 40-44
TGTCT 10758945 3.558719 20.895475 180-189
TCTTG 10729845 3.5490937 19.434425 170-179
GCCCC 12705465 3.5461218 73.54859 60-69
AGATA 6555225 3.5252252 18.507723 150-159
AGGCT 10521240 3.4091008 43.840347 30-34
GAGGA 10120990 3.3996305 129.1818 35-39
TGTCC 10607985 3.3156598 19.157795 120-129
GCCCT 11169000 3.2988634 33.10167 20-24
CTTCA 7742315 3.2891564 16.732782 200-209
ATGCA 7405760 3.2615132 78.36996 25-29
CCAGG 10578480 3.2389867 38.65889 50-59
TCAGG 9974785 3.2320378 20.331083 140-149
CCGTG 12033990 3.1932492 24.06382 120-129
CTCTC 9172450 3.1911657 13.689542 100-109
GGGTT 12571260 3.1714802 42.32574 50-59
GATGG 10863620 3.1624267 32.869987 15-19
TTCCC 9027705 3.1408076 54.415874 50-59
ACAGA 6173455 3.137188 14.569563 140-149
CCTGG 11756915 3.1197267 31.012268 50-59
TCGCA 8443760 3.0453393 24.507647 100-109
GGATG 10375530 3.0203426 56.911377 20-24
TACAC 6123750 3.001884 22.57956 110-119
GACTC 8295080 2.9917161 21.24053 190-199
TGCAC 8287630 2.9890292 101.43522 25-29
CGCAC 8753385 2.9832447 24.936464 100-109
TGGAG 10191075 2.9666476 31.201952 25-29
GGTTC 10506085 2.950197 47.611618 50-59
TCTCA 6942465 2.9493573 20.709206 130-139
TCTCT 7881230 2.9016497 15.556663 180-189
CCAGT 7989000 2.881325 40.438435 60-69
TGGCC 10694645 2.8378506 38.40981 60-69
CAGAT 6432200 2.832755 15.573909 150-159
TTCTT 7260905 2.8289723 21.613802 170-179
CCTGA 7746610 2.7939045 66.076126 30-34
GCCGT 10482925 2.78167 22.777103 120-129
TCACA 5631090 2.7603803 12.331279 130-139
CGGAT 8479560 2.7475536 64.245 20-24
AGGGT 9437125 2.747171 50.200882 50-59
GCACT 7607265 2.7436473 71.8569 25-29
GTTCC 8744780 2.7332914 54.180992 50-59
CGGTG 11437000 2.7265177 67.9602 40-44
GGTTG 10794485 2.7232351 58.158005 15-19
ACCAC 5849065 2.7094173 15.793264 230-239
AGGGA 7968565 2.6766331 9.884163 150-159
AATAA 3653140 2.6701903 12.987598 110-119
TCAAA 4417275 2.6441164 54.856926 70-79
CGGCC 10493305 2.6311674 10.991832 120-129
GGCTG 10784740 2.5710225 24.30674 30-34
AGTCA 5782725 2.546725 29.042679 70-79
AGATG 6427895 2.5432637 16.947933 180-189
GTTGG 9869130 2.4897864 45.31425 15-19
TGTTC 7465045 2.4692013 12.832698 140-149
GATGC 7566665 2.4517567 74.3312 25-29
GAGAT 6137170 2.428235 16.521048 180-189
CTGGC 9081900 2.4099045 34.0832 60-69
ACTCT 5635060 2.3939342 13.204797 200-209
ACAGC 5669155 2.3592873 12.391634 120-129
CACTA 4795710 2.3508742 15.288778 240-247
GTCTC 7510820 2.3476017 12.557547 180-189
CAGAG 6277250 2.3469563 9.405491 140-149
GTTCT 7065250 2.3369615 18.333885 160-169
TTGTC 7062760 2.336138 14.939898 170-179
TCATT 5195480 2.33575 16.450062 150-159
TGTAG 6796250 2.3303933 8.880875 210-219
TCCAT 5456470 2.3180645 14.051395 70-79
CATTT 5150625 2.3155844 17.55661 150-159
TTCAT 5121855 2.3026505 17.13724 140-149
CTGGA 7070105 2.2908611 15.02531 180-189
TCCTC 6579060 2.2889054 14.908552 130-139
AAAAA 2709870 2.2855308 9.034309 230-239
GGCGG 10930455 2.2121804 36.12867 20-24
GTGAA 5577425 2.2067664 17.120005 190-199
ATATA 3471560 2.1990635 10.517822 110-119
TTCAC 5131135 2.1798527 17.319723 200-209
TTGGT 7330460 2.1783533 40.516045 25-29
GCGGA 7826005 2.1527772 77.23103 20-24
TAGTC 5612550 2.1421359 24.61898 70-79
AATAT 3360660 2.128814 12.485002 110-119
ATTTG 5262620 2.125572 15.425523 150-159
TGAAT 4552620 2.121768 14.633215 190-199
TTGGA 6178555 2.1185892 16.911016 170-179
GATAC 4783770 2.106783 13.427884 150-159
GAATC 4760405 2.096493 15.165134 190-199
CTCTG 6699010 2.0938601 14.902746 180-189
GAGTC 6460510 2.09334 12.76798 210-219
ACTAC 4266085 2.09125 12.225374 230-239
CAGCC 6112250 2.083118 11.26079 120-129
TCTGC 6649620 2.078423 10.585535 210-219
ATATG 4433885 2.066431 9.979596 180-189
GATAT 4430970 2.0650725 9.797459 180-189
GTTCA 5375765 2.051762 15.457466 140-149
CTCGC 6933820 2.0479655 16.787601 100-109
TCTCG 6530130 2.0410748 16.563332 100-109
CTACT 4786380 2.0333908 8.180991 230-239
TCGGC 7652335 2.030566 8.553239 190-199
AATAG 3760795 2.0224552 8.517663 240-247
CCTTC 5768780 2.0070028 13.6025715 200-209
ATAGA 3723160 2.002216 10.195163 240-247
AATCG 4531910 1.9958634 13.725789 190-199
CCTTG 6383070 1.9951092 41.363728 20-24
ACACA 3527015 1.9950166 13.760562 110-119
GTCAA 4517730 1.9896183 39.07163 70-79
CACGG 6493490 1.9882183 7.575975 200-209
TTGGG 7836350 1.9769561 71.73321 15-19
TACAG 4479550 1.972804 11.903719 160-169
TCCCA 4877300 1.9579709 12.04194 240-247
GTTGT 6536085 1.9422933 7.7491674 210-219
TTTGC 5837700 1.9309269 11.756014 150-159
TTGCA 5054450 1.9291263 13.471754 150-159
GCTGA 5863535 1.8999074 38.366043 30-34
GGTCA 5862755 1.8996546 9.826519 130-139
TACCA 3868345 1.8962766 11.937333 230-239
GAACT 4292180 1.8902856 11.4648695 160-169
GTCAC 5221530 1.8832049 9.41386 140-149
GTCCT 6021145 1.881985 12.85811 130-139
TGGAA 4734920 1.8734205 9.158557 25-29
AGTAT 3997570 1.8630846 11.990014 220-229
GCTGT 6625730 1.8605608 9.801712 140-149
ATTGT 4601455 1.8585273 8.393702 170-179
ATGTA 3967330 1.8489913 12.008993 70-79
TACTA 3546220 1.8396242 10.575519 230-239
CAGGC 5958060 1.8242769 13.979443 140-149
AGTCT 4767650 1.8196638 13.09727 210-219
CTGTT 5416955 1.7917575 12.539149 140-149
CATAG 4067885 1.7915055 8.136862 210-219
TCAGC 4945795 1.7837579 11.642458 140-149
TTGTA 4375915 1.7674317 10.143616 210-219
GTCTG 6273335 1.7616053 8.861361 210-219
AGAAC 3454035 1.75525 11.223392 160-169
TGGGG 8156445 1.7469088 43.06887 15-19
CCAGA 4146655 1.7256807 24.740625 60-69
CAGCT 4766455 1.7190768 9.426706 150-159
GACGT 5300500 1.7174721 10.287003 170-179
CACCA 3701500 1.7146173 11.280118 230-239
ATAGT 3678695 1.7144716 7.1094403 220-229
GCAGA 4561510 1.7054704 13.687257 150-159
TTCAG 4443080 1.6957855 10.237837 150-159
TCTGG 6018485 1.6900414 12.171132 180-189
GTGGA 5779320 1.6823746 38.487232 25-29
CCCAC 4433210 1.681737 11.092959 240-247
ACGGC 5471535 1.6753097 12.326172 120-129
ATCTC 3925830 1.6678047 7.1026244 100-109
TGACT 4349030 1.6598896 12.191024 190-199
GAGAA 3625770 1.6553339 10.9849825 160-169
TGCAG 5087725 1.6485288 12.607942 150-159
GGACG 5954300 1.6379086 8.112888 170-179
CTCCA 4078280 1.6372077 8.578571 240-247
GGAGG 6609115 1.6333351 26.732405 30-34
ATAAT 2563540 1.6238775 5.7690125 110-119
GTCCC 5490690 1.6217241 18.034273 50-59
AACTG 3665085 1.6141114 10.8212 160-169
GGGGC 7962525 1.6115104 39.769577 15-19
CGACT 4415675 1.5925639 9.708704 190-199
TGGTT 5320010 1.5809189 16.545801 10-14
AACCA 2794320 1.5805758 12.101852 70-79
ATAAA 2162290 1.5804831 6.557484 110-119
CACTG 4354270 1.5704176 6.840556 230-239
TCGAC 4346060 1.5674566 10.188787 190-199
CTCTT 4249960 1.5647172 9.248624 200-209
ACGGA 4183560 1.5641614 10.102414 200-209
AGCTC 4315280 1.5563555 9.616241 140-149
CAAAA 2253135 1.5562383 20.582436 70-79
GGGCG 7641815 1.546603 46.70525 20-24
CAAAG 3020890 1.535137 27.586655 70-79
ACTGA 3484370 1.5345243 7.5610676 180-189
ATGGG 5221665 1.5200397 53.141605 15-19
CCTCG 5083990 1.5016019 9.178512 130-139
CTGGT 5305070 1.4897083 7.101046 160-169
ACACG 3552185 1.4782847 9.435802 120-129
GCTCT 4716450 1.4741861 9.257748 130-139
TCAGA 3344185 1.4727865 8.986389 130-139
TGGAC 4528110 1.467202 11.249811 170-179
AAATA 2004855 1.4654089 5.458278 6
AGCCG 4777165 1.4627029 10.840619 120-129
CTCGA 4049505 1.4605008 9.341428 190-199
TCCAC 3627275 1.4561538 10.499174 240-247
CCACC 3820580 1.4493359 8.340131 230-239
ATCGG 4471050 1.4487132 9.471757 190-199
GAGGG 5848880 1.4454556 9.52622 200-209
AATTG 3088930 1.439609 9.315954 170-179
GGCCG 6345815 1.4295411 11.825834 120-129
CCATC 3522065 1.4139177 10.854701 240-247
CAGAC 3393310 1.412167 13.233916 60-69
CGTAG 4345320 1.4079741 8.397306 210-219
CTTGA 3670345 1.4008566 9.58795 200-209
GGGAG 5637000 1.3930929 7.0307446 150-159
TAGTT 3430125 1.3854274 8.531157 220-229
CAATA 2309940 1.3826964 7.7586617 100-109
TATGG 4019575 1.3782881 7.1170955 180-189
CATGT 3603045 1.3751702 11.483176 70-79
GAATT 2904190 1.3535101 10.443606 170-179
ACTAT 2606935 1.3523641 7.259966 230-239
ACCAT 2734245 1.3403366 10.788831 45-49
ACGTG 4089185 1.3249809 9.722177 170-179
GGCTC 4971080 1.3190883 8.346259 130-139
TATAC 2536845 1.3160045 6.5251474 110-119
ACTCG 3648060 1.3157148 9.477841 190-199
CCATG 3647745 1.3156013 11.520278 70-79
GTGCT 4660920 1.3088256 7.449378 220-229
GAGGC 4731000 1.3014033 23.526363 30-34
GTCCA 3582400 1.2920338 12.935073 70-79
TGATA 2769795 1.290875 7.079279 180-189
ACTGT 3341120 1.2752016 5.365757 230-239
AAGTA 2359835 1.2690563 16.86664 70-79
GCTCA 3515770 1.268003 9.473777 140-149
ACTTC 2981725 1.2667218 5.633166 210-219
TTCCA 2961860 1.2582827 7.3224516 230-239
GTGTT 4226710 1.2560288 7.369888 160-169
ATCCA 2558255 1.2540658 16.38936 240-247
GGTGG 5831470 1.2489566 38.417522 25-29
GGAGT 4258875 1.2397691 6.994056 210-219
GGGCC 5501375 1.2393115 29.614063 15-19
AAAGT 2299945 1.236849 20.573086 70-79
TGTGC 4397850 1.2349533 6.321946 220-229
TTGAG 3581685 1.2281383 8.708501 200-209
AGAGA 2676905 1.2221326 13.105092 35-39
CCTCA 3043870 1.2219484 5.8586583 130-139
TATCA 2343145 1.2155213 5.8797545 70-79
GGAAT 3070030 1.2146893 8.359775 170-179
GAACC 2917880 1.214311 9.020954 70-79
GCGTA 3713690 1.2033129 9.193604 210-219
CTGAT 3149815 1.2021865 6.1671705 180-189
TGGGC 5040070 1.2015251 31.778046 15-19
TGCCC 4029250 1.1900748 10.789704 60-69
ACTGG 3658910 1.185563 7.1818933 160-169
AGTTC 3081245 1.1760154 5.6636434 160-169
CTGCG 4404300 1.1686919 6.343525 210-219
GTCTA 3014480 1.1505332 7.678792 180-189
ACAAT 1918745 1.1485327 8.771452 100-109
TCACG 3176920 1.1457928 8.691707 200-209
CTGAA 2578225 1.1354561 13.892344 35-39
ATCAT 2151670 1.1161925 5.038332 90-99
TATGT 2755050 1.1127645 7.345756 220-229
GCATA 2526435 1.1126475 7.547074 210-219
TAGAT 2380580 1.1094795 8.260381 240-247
CCCAA 2382060 1.1034234 9.99644 240-247
AGCTT 2873790 1.0968362 6.0674276 150-159
GCGGT 4590020 1.0942354 7.088002 130-139
TCTAC 2575600 1.0941885 7.289283 180-189
GAAGA 2382475 1.0877115 18.483997 35-39
AGAGC 2896945 1.0831181 6.448894 140-149
TGGTC 3845215 1.0797688 9.398358 50-59
TTGGC 3841970 1.0788575 5.0011845 170-179
AGATC 2438720 1.0740178 5.3925514 130-139
CTCGG 4027505 1.0687084 7.6331987 130-139
AAAAG 1719475 1.0669837 13.533054 70-79
AGGTT 3111605 1.0669507 18.243681 10-14
CGGCT 4007930 1.0635141 7.4862404 130-139
GTGGT 4208160 1.0616356 8.287458 50-59
CCAAT 2162315 1.0599746 11.254381 240-247
TATAA 1639310 1.0384228 7.517648 240-247
ATAAC 1720985 1.0301566 7.039316 80-89
CGGTC 3806400 1.0100377 7.2135944 130-139
AAGAG 2209360 1.0086763 12.968785 35-39
CATCA 2043720 1.0018388 5.728781 230-239
TACTG 2614705 0.9979516 5.254918 180-189
ACCGT 2747190 0.99080575 14.707448 45-49
TCTGT 2972940 0.9833547 5.393912 210-219
ATATC 1888835 0.97984517 5.793155 70-79
CGGAG 3550400 0.9766439 6.5145025 210-219
GCTTC 3097820 0.9682628 5.5145965 150-159
CCATT 2273700 0.9659327 5.0503306 50-59
TGTAA 2064590 0.962211 6.5537143 230-239
CGATG 2965515 0.96088845 22.381287 15-19
GAGTT 2797865 0.95937115 5.215494 160-169
ACCCA 2033590 0.9420042 11.77354 240-247
ACTAA 1564145 0.9362744 11.554843 240-247
TAGAC 2106335 0.92763466 5.00455 110-119
TGAAG 2307330 0.91291916 9.746575 35-39
TCCAG 2528850 0.912059 5.544683 240-247
CTACC 2254480 0.9050512 6.8572474 230-239
ATCAA 1493020 0.8937001 13.239786 70-79
CTTCC 2561650 0.89121765 5.1190085 230-239
CCAGC 2601230 0.8865262 5.708502 80-89
GAGCT 2726870 0.8835626 5.509607 140-149
CCTTT 2339135 0.8612045 6.9799747 50-59
AGCTG 2632485 0.85297984 7.2583146 30-34
TGCGT 3024815 0.8493935 6.782629 210-219
AATCC 1725405 0.8457997 10.614797 240-247
TGCAT 2213235 0.844723 6.1392393 210-219
TGAAC 1865570 0.8216012 5.262712 190-199
CACAA 1441055 0.81511664 5.043749 100-109
ACGTC 2246955 0.81039023 8.405366 70-79
AGCCA 1937565 0.80634105 7.464659 80-89
GTATG 2332250 0.79971457 5.0243177 220-229
GGAAG 2320820 0.77956104 10.113042 30-34
AGACC 1839655 0.76559466 5.2859526 240-247
TAACC 1546435 0.7580679 6.428095 80-89
TAGGT 2203330 0.75550854 6.1460032 10-14
CAATC 1524635 0.74738145 10.61805 240-247
CTAAT 1434120 0.74395883 9.122483 240-247
AAAGG 1629100 0.7437604 7.218379 10-14
AGGAT 1873425 0.7412401 8.532066 10-14
CGGCG 3282300 0.73941374 5.0444245 130-139
TCGAA 1675700 0.73798203 12.800563 70-79
GGTCC 2773810 0.7360373 10.297635 50-59
TGAGA 1853680 0.73342776 5.995829 240-247
GACCG 2381490 0.7291798 14.727863 45-49
GCGGC 3234790 0.728711 5.2452555 130-139
AAATG 1346230 0.72396654 5.406942 9
ATACC 1470290 0.7207414 5.864612 240-247
CTTTG 2072735 0.6855952 6.1083508 50-59
GTCGA 2109620 0.6835608 8.643104 70-79
ACGAT 1542080 0.67913544 8.826629 10-14
TGGAT 1959050 0.6717465 6.8872533 10-14
CGAAC 1612735 0.67115915 8.886632 70-79
CGTGG 2812680 0.6705274 5.5162945 50-59
AAAGC 1280615 0.65077496 9.19505 70-79
AATGG 1640110 0.6489266 5.5774417 10-14
CGTCC 2146200 0.6338993 6.687731 70-79
AAGGT 1599690 0.632934 6.5393553 10-14
TTGCC 1993055 0.62295455 7.2015696 60-69
CATCC 1476415 0.59270036 7.1251054 240-247
GAAGC 1575535 0.58906555 9.2714 30-34
ATAGG 1439285 0.5694681 5.433912 10-14
AAGCT 1261100 0.55539125 14.971487 30-34
TCAAG 1200160 0.52855307 5.7743855 70-79
GACGA 1409080 0.5268309 6.4253025 40-44
AAGCA 957280 0.4864646 5.950432 70-79
CGGTT 1689580 0.47444826 9.599828 10-14
GGGAT 1608445 0.46822238 5.983139 10-14
TAAAG 840830 0.45217595 5.2196946 9
TCGAT 1127690 0.4304042 6.753443 10-14
GCGAT 990535 0.3209539 5.184165 10-14