FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_3_SL254630.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_3_SL254630.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2727945
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2975375 7.7523184 54.162346 110-119
CACAG 3956390 6.7528906 37.743793 110-119
TCCCT 4269055 6.3484526 89.364174 30-34
CAGTA 3389275 6.1665883 40.709087 60-69
ATACA 2435505 5.952938 29.408627 110-119
GGAGA 4279080 5.881687 89.35351 35-39
GGTGA 4651220 5.6530313 88.55052 45-49
CCCTG 4387265 5.4924088 53.99746 30-34
AGTAA 2493370 5.4690204 39.42024 110-119
GTAAT 2697015 5.230812 34.739 110-119
GGCCC 4550565 4.7958674 55.928295 60-69
AGACG 3088150 4.7300906 92.62823 40-44
GTGAC 3482705 4.7168355 78.46733 45-49
ACAGT 2589295 4.7110715 31.141272 110-119
GAGAC 3059495 4.6861997 121.02252 40-44
CCCCA 2957165 4.6655316 95.30803 60-69
GTAGT 3204855 4.626899 23.39469 70-79
AAAAA 1348575 4.4940615 24.06923 230-239
ACTCC 2614390 4.396869 90.709564 30-34
GTGTC 3617640 4.332338 24.368408 120-129
ACCAG 2518255 4.2982364 93.526634 45-49
GCACA 2446580 4.1758995 31.370842 110-119
CGTGT 3469920 4.1554346 23.424147 120-129
AATAC 1689430 4.1293573 29.279114 110-119
CCCTT 2734975 4.0671434 64.395744 20-24
TGACC 2676825 4.0399275 145.05682 45-49
TGGTG 3701900 3.9783418 28.938602 25-29
CACTC 2335255 3.9274209 79.382385 25-29
GACCA 2287560 3.9044795 103.61292 45-49
CTCAG 2560285 3.8640425 28.93426 140-149
CCACT 2289135 3.8498569 26.93814 240-247
CCCAG 2718775 3.8492787 79.33767 60-69
TGAGG 3145975 3.823576 67.098785 35-39
TAGTA 1958095 3.79769 24.0933 80-89
AGTAG 2248080 3.6705437 25.872591 70-79
CAGGG 3176895 3.6221776 32.82672 50-59
CTCCC 2574340 3.591323 53.673737 30-34
CTTGG 2998820 3.5912645 37.72941 20-24
CTGAG 2641270 3.5772297 94.10411 35-39
AGGAG 2601335 3.5755908 88.96249 35-39
GACGG 3115460 3.5521321 106.84329 40-44
TGTCT 2469785 3.513365 20.236916 170-179
TCTTG 2431690 3.4591734 18.4198 170-179
GCCCC 2943810 3.4572532 70.826515 60-69
ATGGT 2386770 3.4458163 23.768127 190-199
ACGGT 2531715 3.4288526 91.02839 40-44
AGGCT 2482370 3.3620222 43.844685 30-34
CTTCA 1870435 3.3532298 15.955145 200-209
GAGGA 2402850 3.302769 122.3919 35-39
TTCCC 2214095 3.2925496 55.75359 50-59
GCCCT 2607930 3.2648623 33.705757 20-24
CCTGG 2900715 3.2587757 32.04088 50-59
AGATA 1483220 3.2533317 16.709978 150-159
CCAGG 2546520 3.235442 39.090736 45-49
GGGTT 3000095 3.2241292 43.200096 50-59
ATGCA 1767150 3.2152267 75.16107 25-29
TGTCC 2405870 3.2106183 18.941494 120-129
TCAGG 2327325 3.1520352 19.14475 140-149
CTCTC 2103245 3.1277065 13.291795 100-109
GATGG 2550690 3.1000745 32.981735 15-19
CCAGT 2046030 3.0879169 40.57891 60-69
GGATG 2512965 3.054224 56.915604 20-24
GGTTC 2526815 3.0260105 48.715153 50-59
CCGTG 2693200 3.0256455 23.210953 120-129
TGCAC 1987820 3.0000646 100.31539 25-29
ACAGA 1439825 2.9626813 13.64546 140-149
TGGAG 2406430 2.924743 31.45004 25-29
GACTC 1936090 2.9219928 20.879059 190-199
TCGCA 1925425 2.9058964 23.495508 100-109
TCTCA 1608175 2.8830621 20.142061 130-139
TACAC 1417850 2.8746703 21.717493 110-119
TGGCC 2530045 2.8423507 37.889683 60-69
TCTCT 1787680 2.8338275 14.43076 180-189
CGCAC 1997065 2.8274713 24.082327 100-109
GTTCC 2096310 2.7975123 55.42687 50-59
AGGGT 2297395 2.792223 50.3247 50-59
GCCGT 2481770 2.7881165 21.742496 120-129
TTCTT 1640365 2.7718625 20.847015 170-179
CGGAT 2025920 2.7438242 64.20483 20-24
CAGAT 1506325 2.7406707 14.639295 150-159
GCACT 1815640 2.7402065 71.80553 25-29
GGTTG 2541525 2.7313151 58.385216 15-19
AGTCA 1500365 2.7298272 28.410887 70-79
CGGTG 2648235 2.6698468 66.71924 40-44
CCTGA 1765535 2.664587 63.35836 30-34
AGGGA 1932030 2.6556168 9.603236 150-159
TCACA 1294650 2.624884 12.257509 130-139
ACCAC 1369405 2.6046062 13.772758 230-239
GGCTG 2555320 2.576174 24.598824 30-34
GTTGG 2348625 2.5240102 44.973717 15-19
AATAA 856415 2.5235457 11.624467 110-119
TCAAA 1027900 2.5124254 51.592827 70-79
CGGCC 2370610 2.4983997 10.252675 120-129
CTGGC 2208285 2.480873 34.18528 60-69
GATGC 1804630 2.4441183 72.97451 25-29
ACTCT 1338765 2.4000764 12.979705 190-199
AGATG 1468990 2.3984876 15.771531 180-189
TGTTC 1667310 2.3718133 12.131086 140-149
GAGAT 1430775 2.336092 15.340357 180-189
GTCTC 1742875 2.3258557 11.651844 180-189
CAGAG 1487980 2.279125 8.855102 140-149
GTTCT 1602090 2.2790353 18.090382 160-169
TCCTC 1529920 2.275123 14.006833 130-139
TCCAT 1260440 2.2596588 11.534698 70-79
TTGGT 1768510 2.257626 42.103176 25-29
TTGTC 1576415 2.2425113 14.639017 170-179
CTGGA 1647020 2.2306576 13.822364 180-189
TGTAG 1542865 2.227458 8.850021 210-219
TCATT 1160225 2.2172284 15.335649 150-159
TTCAT 1158670 2.2142568 16.542768 140-149
CATTT 1154690 2.206651 16.37014 150-159
GGCGG 2593390 2.2010536 35.194904 20-24
ACAGC 1275330 2.1767733 11.421397 110-119
GCGGA 1889725 2.1545944 75.67007 20-24
TTCAC 1197215 2.1463118 15.90709 200-209
TCTGC 1604425 2.141095 10.962927 210-219
ATATA 818295 2.132062 10.0585375 110-119
TAGTC 1317610 2.1197665 24.117483 70-79
TCTCG 1580390 2.1090205 16.42956 100-109
GATAT 1082925 2.1003134 9.833407 180-189
CACTA 1025920 2.0800378 13.437765 240-247
GTGAA 1271965 2.0767958 15.353845 190-199
TTGGA 1429475 2.063755 16.042337 170-179
CTCTG 1541095 2.0565817 14.323615 180-189
TGAAT 1056835 2.0497124 13.444923 190-199
GAGTC 1503150 2.035806 11.635222 210-219
GAATC 1114235 2.0272858 13.993819 190-199
ATTTG 1179510 2.0227876 14.2625265 150-159
TTGGG 1874505 2.014485 72.33779 15-19
CCTTG 1508520 2.0131106 42.640667 20-24
CAGCC 1421380 2.0124087 10.66252 120-129
CCTTC 1343200 1.997454 12.6320715 200-209
GTTCA 1239490 1.9940873 14.501245 140-149
GTCAC 1319585 1.9915485 9.419171 130-139
ATATG 1024550 1.9870964 9.923004 180-189
AATAT 760140 1.9805392 11.769231 110-119
CTCGC 1579515 1.9773918 16.344734 100-109
GAACT 1084125 1.9725026 11.510121 160-169
ACTAC 972275 1.9712734 11.020278 230-239
CTACT 1098215 1.9688293 7.708069 230-239
AATAG 889970 1.9520823 7.7099676 240-247
GATAC 1072385 1.9511423 12.385887 150-159
GTCAA 1068990 1.9449651 38.45846 70-79
TCCCA 1149410 1.9330726 12.031871 240-247
ATAGA 879810 1.9297974 9.268825 240-247
TCGGC 1716550 1.9284389 8.154898 190-199
ACACA 837230 1.9197272 12.616817 110-119
GTTGT 1491240 1.9036719 7.815321 210-219
GCTGA 1403580 1.9009523 37.82611 30-34
CTCCA 1128290 1.8975531 8.483266 240-247
TCAGC 1246970 1.8819563 11.537508 140-149
AATCG 1033045 1.8795652 12.621095 190-199
CACGG 1472985 1.8714783 7.0033693 200-209
GTCTG 1557525 1.8652284 8.338871 210-219
GGTCA 1372325 1.8586217 9.840103 130-139
TACAG 1019045 1.854093 10.573587 160-169
GCTGT 1534125 1.8372055 9.154347 140-149
TTGCA 1141550 1.8365215 12.289335 150-159
GTCCT 1368505 1.8262613 11.85256 130-139
TTCAG 1127570 1.8140306 10.086395 150-159
ATCTC 1002625 1.79746 6.8309836 100-109
TTTGC 1257300 1.7885579 11.121822 150-159
CACCA 938885 1.7857577 10.354409 230-239
TGGAA 1092490 1.7837588 9.071426 25-29
TGGGG 1968610 1.781025 43.484398 15-19
AGTCT 1102235 1.773272 12.147369 210-219
CATAG 974210 1.7725183 8.158663 210-219
ATTGT 1029520 1.765564 7.991178 170-179
AGTAT 907845 1.760749 10.91776 220-229
CAGGC 1384170 1.7586358 12.76851 140-149
TACCA 866925 1.7576778 10.740327 230-239
CTGTT 1227500 1.7461663 11.725276 140-149
TTGTA 1015485 1.7414948 10.189784 210-219
TACTA 802255 1.7338748 9.552167 230-239
CCAGA 1004855 1.7151182 21.154163 60-69
GTGGA 1411095 1.7150261 38.822388 25-29
ATGTA 878750 1.7043197 9.396484 70-79
ATAGT 874245 1.6955824 6.727484 220-229
CCCAC 1062710 1.6766421 10.324221 240-247
CAGCT 1107940 1.672129 9.030691 140-149
GGAGG 1628460 1.6661896 26.974136 30-34
TGACT 1035175 1.6653861 11.952727 190-199
TCTGG 1374895 1.6465181 11.328803 180-189
AGAAC 798405 1.6428521 10.499548 150-159
GGGGC 1918870 1.628577 40.05784 15-19
ACGGC 1262515 1.6040689 11.479126 120-129
CACTG 1055985 1.5937173 6.2185497 230-239
GACGT 1174700 1.5909665 10.081024 170-179
GCAGA 1036475 1.587559 12.396303 150-159
TGGTT 1241910 1.5853847 16.743063 10-14
TGCAG 1170400 1.5851427 11.441028 150-159
CTCTT 995020 1.5773042 8.99788 200-209
GAGAA 852650 1.5744413 10.146154 160-169
ACACG 918230 1.5672637 9.364748 110-119
GGACG 1372445 1.564811 7.7402287 170-179
ATAAT 596440 1.5540202 5.053339 100-109
CGACT 1026700 1.5495197 9.736599 190-199
ATGGG 1272500 1.5465794 53.408154 15-19
GGGCG 1818520 1.5434083 46.130432 20-24
CAAAG 748145 1.5394336 26.798462 70-79
AACTG 843165 1.5340896 10.167015 160-169
ACTGA 842705 1.5332527 7.434352 180-189
ATCGG 1127110 1.5265124 8.921565 190-199
AGCTC 1010660 1.525312 9.567654 140-149
ATAAA 511980 1.5086203 7.6981397 8
TCGAC 998465 1.5069067 10.339216 190-199
CTTGA 930740 1.4973713 9.542592 200-209
GTCCC 1194210 1.4950291 15.166443 50-59
AACCA 650605 1.4918052 10.990643 70-79
CTGGT 1241315 1.4865482 7.125973 160-169
TCAGA 816065 1.4847828 8.445267 130-139
TCCAC 880155 1.4802406 10.836108 240-247
CCACC 932210 1.4707518 7.692831 230-239
CAAAA 531690 1.469731 17.97249 70-79
GAGGG 1428420 1.4615148 9.430554 200-209
ACGGA 945425 1.4480985 9.320603 200-209
TGGAC 1068485 1.4471132 10.715585 170-179
CCATC 847615 1.425515 11.155429 240-247
AAATA 483760 1.425466 5.0496297 6
GCTCT 1066435 1.4231508 8.717503 130-139
CTCGA 936385 1.4132143 9.285382 190-199
CCTCG 1121380 1.4038535 8.396435 120-129
ATCCA 688080 1.3950722 16.907373 240-247
AGCCG 1095330 1.3916547 10.040136 120-129
TAGTT 807360 1.3845731 8.351991 220-229
GGCCG 1455435 1.3764966 11.047184 120-129
GGGAG 1333695 1.3645952 6.9435077 150-159
GAGGC 1195995 1.3636291 23.617825 30-34
AATTG 700220 1.3580639 8.828101 170-179
CGTAG 990935 1.3420825 7.726422 210-219
CAATA 549000 1.3418831 7.1639824 100-109
TATGG 917080 1.3240026 7.1605687 180-189
GGTGG 1440955 1.3036493 39.14832 25-29
CAGAC 763655 1.3034303 10.487509 60-69
GGCTC 1155115 1.297701 7.889264 130-139
ACTAT 600325 1.2974533 7.0129123 230-239
CATGT 803960 1.2934082 9.360668 70-79
GAATT 665505 1.2907349 9.824255 170-179
AGATC 707415 1.2871004 6.2654076 130-139
ACTCG 852745 1.2869829 9.378983 190-199
GGAAT 783835 1.2798034 7.8276134 170-179
CTGAA 703400 1.2797954 11.757734 35-39
ACTTC 709670 1.2722638 5.414117 220-229
TTCCA 709575 1.2720933 7.238451 230-239
ACGTG 938135 1.2705722 9.530447 170-179
GTGTT 992870 1.2674677 7.129511 160-169
GTGCT 1054975 1.263395 7.1535354 220-229
ACTGT 784020 1.2613287 5.146769 230-239
CCTCA 748290 1.2584708 5.9318333 130-139
GGGCC 1326200 1.2542708 29.956476 15-19
CCATG 826595 1.2475166 9.333835 70-79
TGGGC 1232050 1.2421048 32.421326 15-19
TATAC 574165 1.240915 6.050579 110-119
GCTCA 819480 1.2367784 9.379036 140-149
ACCAT 609070 1.2348804 9.268975 45-49
TGATA 631750 1.2252678 7.1045938 180-189
TTGAG 841115 1.2143306 8.629572 200-209
AAGTA 551295 1.2092241 16.08563 70-79
GAAGA 654775 1.2090597 15.606666 35-39
TCCAG 801000 1.208888 5.5367823 240-247
TGTGC 1002265 1.2002716 5.751966 220-229
GTCCA 794690 1.1993648 10.502239 70-79
AAAGT 546335 1.1983447 19.66166 70-79
AGAGA 647615 1.1958386 11.049972 35-39
CTGAT 741800 1.1934053 6.0192313 180-189
GGAGT 970840 1.1799458 6.2844243 210-219
AGAGC 761305 1.1660837 6.906238 140-149
AGTTC 716895 1.1533383 5.2981324 160-169
TATCA 531705 1.1491482 5.2501383 70-79
ACTGG 845395 1.144969 6.9852705 160-169
GCGTA 845200 1.1447047 8.475591 210-219
TAGAT 583665 1.1320076 7.794849 240-247
GTCTA 702845 1.1307347 7.4746094 180-189
AAGAG 611075 1.1283665 10.964702 35-39
GAACC 656920 1.1212518 8.285557 70-79
GCATA 611515 1.112616 7.463734 210-219
CCCAA 583125 1.109103 9.457778 240-247
TGCCC 883235 1.1057202 8.6367035 60-69
CTGCG 984210 1.1056997 5.8375754 210-219
ACAAT 448625 1.0965432 8.041408 100-109
AGGTT 754620 1.0894566 18.643677 10-14
ATATC 503355 1.0878768 5.4401407 70-79
GCGGT 1067880 1.0765948 6.900795 130-139
TCTAC 597475 1.0711257 7.023266 180-189
TCACG 707240 1.0673833 8.114177 200-209
TATGT 620170 1.0635537 7.0809345 220-229
CCAAT 521235 1.0567964 10.820068 240-247
TCTGT 739710 1.0522662 5.2751765 210-219
CTTCT 663105 1.051153 5.1583533 210-219
AAAAG 422600 1.048309 11.586944 70-79
ACCCA 542385 1.0316154 11.204411 240-247
TGGTC 854805 1.0236796 7.7645082 50-59
TATAA 391355 1.0196726 6.8920283 240-247
CGGCT 902130 1.0134878 6.898926 130-139
GTGGT 941875 1.0122102 6.828849 50-59
TAGGC 744980 1.0089709 5.264089 160-169
CTCGG 894910 1.0053766 7.0561423 130-139
TACTG 619855 0.99722064 5.296908 180-189
TGTAA 509170 0.987526 6.540986 230-239
CGGTC 869475 0.9768019 6.99703 130-139
ATAAC 397370 0.97126424 6.862921 80-89
GCTTC 722650 0.96437186 5.4764013 150-159
CTTCC 642145 0.9549249 5.0361986 230-239
AAAAT 323405 0.9529577 5.486197 5
CGATG 701425 0.9499817 22.524965 15-19
CATCA 468110 0.9490862 5.3989596 230-239
AGCTT 589390 0.9482087 5.5189757 150-159
CCAGC 662300 0.93769324 5.4704213 80-89
GGAAG 675930 0.92908025 9.511175 30-34
TTCTG 646815 0.92011935 5.131787 210-219
CGGAG 798495 0.9104143 5.8036346 210-219
ACTAA 360950 0.88224536 10.292822 240-247
ACCGT 583135 0.880081 12.144934 45-49
ACGTC 579950 0.8752741 6.8827887 70-79
TGAAG 532635 0.8696577 8.634054 35-39
AATCC 425180 0.86204624 10.525249 240-247
TGCAT 528440 0.8501525 6.261584 210-219
GAGCT 617875 0.836825 5.3098655 140-149
AGCTG 614740 0.83257914 6.9964743 30-34
CTACC 491420 0.8264679 5.9336405 230-239
ATCAA 336630 0.82280165 11.599573 70-79
TGCGT 674740 0.8080411 6.379229 210-219
AGCCA 466850 0.7968343 7.3280873 80-89
CCTTT 499120 0.7912043 5.94923 50-59
AAAGG 417435 0.77080494 6.828033 10-14
CCCAT 456610 0.76792467 5.1732426 240-247
AGGAT 464960 0.75916153 8.47672 10-14
TGAGA 463075 0.7560839 5.8631644 240-247
CAATC 366505 0.74308354 10.536202 240-247
TAGGT 511445 0.73838097 5.8561606 10-14
GACCG 578965 0.7355951 12.344493 45-49
TAACC 361300 0.73253053 6.086336 80-89
TGGAT 497720 0.71856606 7.275372 10-14
TCGAA 392095 0.71339405 12.130328 70-79
ATACC 342795 0.6950119 5.7978067 240-247
CTAAT 320370 0.69240016 8.155692 240-247
GGTCC 603995 0.67855144 8.622128 50-59
CATCC 397455 0.66843796 7.923028 240-247
AAGGT 402665 0.6574496 6.371751 10-14
GTCGA 485145 0.65706086 8.330087 70-79
ACGAT 357425 0.650314 8.044701 10-14
CTTTG 441330 0.62780905 5.1323853 50-59
AAAGC 304445 0.62644655 8.171298 70-79
CGAAC 360125 0.6146726 8.256051 70-79
GAAGC 394450 0.6041754 8.799871 30-34
ATAGG 354540 0.5788738 5.542827 10-14
AAGCT 309440 0.56300807 14.147079 30-34
CGTCC 448135 0.5610193 5.474923 70-79
TTGCC 413190 0.55139935 5.917807 60-69
TCAAG 285675 0.519769 5.517462 70-79
GACGA 331040 0.5070509 5.8865905 40-44
CGGTT 406325 0.4865982 9.841026 10-14
GGGAT 395170 0.4802843 6.2694006 10-14
TAAAG 210620 0.46197915 5.575994 9
AAGCA 220155 0.45300582 5.035859 70-79
TCGAT 275045 0.44249147 6.9822335 10-14
GCGAT 243380 0.32962406 5.37699 10-14