FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_12_SL254622.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_12_SL254622.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8794390
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10339375 8.467136 59.56066 110-119
CACAG 13418625 7.290126 42.10899 110-119
TCCCT 14638905 6.681138 99.54793 30-34
CAGTA 11582850 6.6204247 44.68506 60-69
ATACA 8302965 6.462969 33.631245 110-119
GGAGA 14455975 6.2529426 93.412766 35-39
AGTAA 8533820 5.9271703 44.34872 110-119
GGTGA 15582335 5.8355203 94.14515 45-49
CCCTG 14826525 5.739086 59.480984 30-34
GTAAT 9447920 5.6813307 38.187843 110-119
ACAGT 8787760 5.0228314 34.760098 110-119
AGACG 10236695 4.9624 101.573 40-44
GTAGT 11217625 4.953205 25.577118 70-79
GTGAC 11769645 4.939755 84.919174 45-49
GGCCC 14774275 4.850329 59.087 60-69
CCCCA 9614605 4.817479 102.775375 60-69
GAGAC 9880920 4.7899327 131.96793 40-44
AATAC 5838165 4.544386 33.625957 110-119
ACCAG 8351820 4.537411 100.325455 45-49
GTGTC 12257805 4.4541483 25.613054 120-129
ACTCC 8283240 4.366487 100.00315 30-34
CGTGT 11948290 4.3416786 24.703491 120-129
TGACC 9055725 4.2595153 153.91919 45-49
GCACA 7840275 4.2594967 34.579018 110-119
GACCA 7686255 4.17582 111.05682 45-49
TAGTA 6704220 4.031458 25.526585 80-89
CCACT 7596700 4.004579 28.914888 240-247
CACTC 7591025 4.001588 83.34968 25-29
CTCAG 8482450 3.9898655 30.879305 140-149
CCCAG 8844025 3.9540613 84.6588 60-69
CCCTT 8630520 3.9389346 57.455067 20-24
AGTAG 7704570 3.9293785 29.20256 70-79
TGAGG 10449510 3.9132981 70.816536 35-39
CTCCC 9010230 3.9087179 62.448254 30-34
TGGTG 12010170 3.8940914 25.049109 25-29
CAGGG 10683565 3.802943 35.82561 50-59
GACGG 10439375 3.7160203 113.55816 40-44
AGGAG 8498340 3.6759632 93.04862 35-39
CTGAG 8723050 3.66109 100.01773 35-39
TCTTG 8473165 3.6302454 20.228716 170-179
ACGGT 8598220 3.608698 94.40702 40-44
ATGGT 8138535 3.5936158 25.893442 190-199
TGTCT 8377640 3.5893188 21.609503 180-189
GCCCC 9684945 3.563339 75.27141 60-69
CTTGG 9769465 3.5499542 33.293488 20-24
AGATA 5015360 3.4834216 18.251907 150-159
GAGGA 7936225 3.4328196 130.56686 35-39
ATGCA 5988535 3.4228747 84.081474 25-29
CCAGG 8559490 3.4146516 41.503746 50-59
GGGTT 10283680 3.3343062 44.907997 50-59
CTTCA 6007870 3.3319302 16.850563 200-209
TTCCC 7297615 3.3306024 58.414185 50-59
AGGCT 7903630 3.317176 40.8567 30-34
TGTCC 8121340 3.3073075 19.615406 120-129
TCAGG 7755330 3.2549345 20.326815 140-149
CCTGG 9329395 3.2222703 32.706425 50-59
CTCTC 7021590 3.2046258 13.95245 100-109
GCCCT 8228155 3.184974 30.047174 20-24
ACAGA 4819110 3.181466 16.037096 140-149
CCGTG 9159185 3.1634812 24.90016 120-129
TCGCA 6646015 3.126067 25.583687 100-109
GGTTC 8580790 3.1180224 50.76902 50-59
TGCAC 6609775 3.1090205 107.281654 25-29
TACAC 4823830 3.0899923 24.230272 110-119
GACTC 6566000 3.0884302 23.244558 190-199
CGCAC 6892070 3.0813649 26.20231 100-109
GGATG 8109160 3.036847 58.78906 20-24
CCAGT 6436080 3.02732 43.525295 60-69
GATGG 8032305 3.008065 29.269909 15-19
CCTGA 6298385 2.9625533 70.11074 30-34
TGGCC 8513465 2.940457 40.44883 60-69
TCTCA 5289320 2.9334266 20.812986 130-139
GCACT 6138115 2.8871675 75.45961 25-29
GTTCC 7081235 2.8837385 58.060177 50-59
TCTCT 6002320 2.8820696 15.638414 180-189
TTCTT 5695060 2.876915 22.420582 170-179
AGGGT 7657390 2.8676605 53.10974 50-59
TGGAG 7625840 2.8558457 28.515167 25-29
GGTTG 8736720 2.8327315 61.58936 15-19
CGGAT 6744135 2.8305328 66.87089 20-24
TCACA 4394870 2.8152142 13.644398 130-139
GCCGT 8102155 2.798395 23.57395 120-129
AGGGA 6428810 2.780786 10.589025 150-159
TCAAA 3555095 2.767261 57.663475 70-79
CGGTG 8881765 2.7372396 68.15825 40-44
ACCAC 4468200 2.7205367 15.942065 230-239
CAGAT 4739320 2.7088594 15.3584585 150-159
AGTCA 4683665 2.6770484 31.452211 70-79
CGGCC 8143520 2.6734815 11.681256 120-129
AATAA 2775940 2.6256838 12.522775 110-119
GTTGG 8044945 2.6084352 48.092884 15-19
GATGC 6083300 2.5531785 79.400604 25-29
CTGGC 7364735 2.5436983 36.581425 60-69
GGCTG 8088515 2.4927707 22.613953 30-34
AGATG 4871730 2.4846125 16.668648 180-189
TGTTC 5716450 2.4491577 12.8522415 150-159
ACTCT 4383325 2.4309669 14.056829 190-199
GAGAT 4689930 2.3918934 16.208735 180-189
GTTCT 5561245 2.3826616 19.028507 160-169
CAGAG 4870580 2.361091 10.437944 140-149
ACAGC 4311760 2.3425105 12.489646 120-129
GTCTC 5729650 2.3333235 12.572767 180-189
TCATT 3972985 2.3181188 16.215721 150-159
TTCAT 3943380 2.3008451 16.98917 140-149
CATTT 3932520 2.2945087 17.452562 150-159
ATATA 2783825 2.2797341 10.620847 110-119
TGTAG 5150710 2.2743251 9.580399 210-219
GGCGG 8700085 2.2740426 38.56192 20-24
CACTA 3544140 2.2702634 15.392425 240-247
TTGTC 5286170 2.2648082 14.6872225 170-179
TAGTC 4574825 2.2638876 26.886587 70-79
TCCTC 4951250 2.2597308 14.978213 130-139
CTGGA 5376955 2.2567236 15.016187 180-189
GCGGA 6296540 2.2413287 80.95335 20-24
TCCAT 3979880 2.207219 11.461252 70-79
CTACT 3956245 2.1941109 8.765052 230-239
ACTAC 3423370 2.192902 13.011258 230-239
TTCAC 3931775 2.1805396 17.328781 200-209
GTGAA 4232835 2.1587722 17.045424 190-199
TTGGA 4884000 2.1565576 17.847477 170-179
AATAT 2624670 2.1493988 12.496792 110-119
GTCAA 3740585 2.138011 42.304546 70-79
TTGGT 5577825 2.1323586 36.128242 25-29
ATATG 3538240 2.1276546 11.118337 180-189
GATAT 3537175 2.1270144 10.976624 180-189
ATTTG 4038115 2.1023376 15.285923 150-159
CTCGC 5424605 2.0997696 17.633257 100-109
TGAAT 3491350 2.0994582 14.728171 190-199
GAATC 3629885 2.074738 15.110834 190-199
GATAC 3627750 2.0735178 13.389993 150-159
TCTGC 5088955 2.0724092 10.833528 210-219
CAGCC 4634190 2.071893 11.353179 120-129
AATAG 2981395 2.0707297 9.380184 240-247
TCTCG 5075205 2.0668097 17.294855 100-109
GAGTC 4911615 2.061419 12.67341 210-219
CTCTG 5039660 2.0523343 14.832029 180-189
TTGGG 6318005 2.0485044 74.78661 15-19
CACGG 5107385 2.0374978 7.515229 200-209
GTTCA 4094965 2.0264254 15.367088 140-149
TCGGC 5857665 2.023173 8.562892 190-199
ATAGA 2910960 2.021809 11.245113 240-247
TCCCA 3791805 1.9988396 11.792152 230-239
CCTTC 4377510 1.997878 13.691286 200-209
ACACA 2683555 1.9854819 14.088908 110-119
AATCG 3458265 1.9766452 13.74143 190-199
TACAG 3443115 1.9679857 12.041941 160-169
AAAAA 1789295 1.9548092 7.5056005 230-239
GGTCA 4644920 1.9494864 10.799403 130-139
GTTGT 5086305 1.9444547 8.436743 210-219
TACTA 2873710 1.9366527 11.11135 230-239
GAACT 3375010 1.9290589 12.274435 160-169
GTCAC 4089865 1.9237378 10.365217 130-139
TTGTA 3661380 1.9062005 11.290076 210-219
CCTTG 4671145 1.9022613 37.631886 20-24
TACCA 2963685 1.8984425 12.070033 230-239
AGTAT 3128615 1.8813344 12.303657 220-229
TTGCA 3795310 1.8781385 13.355794 150-159
GTCCT 4583265 1.8664736 12.774291 130-139
GCTGT 5114485 1.8584626 9.916004 140-149
AGAAC 2803640 1.8508989 12.295278 160-169
ATTGT 3538590 1.8422732 8.347126 170-179
CATAG 3215230 1.8377334 8.266219 210-219
TCAGC 3877690 1.8239379 12.485613 140-149
TGGAA 3572720 1.8221095 9.354155 170-179
AGTCT 3679830 1.8209924 13.189042 210-219
TTTGC 4239320 1.8162956 11.643467 150-159
GCTGA 4311230 1.8094357 35.121265 30-34
TGGGG 6579550 1.8093175 44.60244 15-19
CAGGC 4527850 1.8063028 13.905535 140-149
CTGTT 4175480 1.7889442 12.434135 140-149
CAGCT 3765195 1.771024 10.240588 150-159
ATAGT 2902290 1.7452381 7.1976986 220-229
GTCTG 4789450 1.7403541 8.973054 210-219
CCCAC 3459130 1.7332264 11.452256 240-247
GAGAA 2936135 1.7295834 12.211265 160-169
ACGGC 4305895 1.7177579 13.125273 120-129
TGACT 3469465 1.7168918 13.350282 190-199
TTCAG 3460590 1.7124999 11.223933 150-159
AACTG 2986535 1.7070179 11.901866 160-169
ATGTA 2829990 1.7017618 9.96779 70-79
GGGGC 6434375 1.6818277 41.283916 15-19
GGACG 4692230 1.6702555 8.7739315 170-179
GACGT 3976855 1.6690974 11.029151 170-179
TCTGG 4586170 1.6664877 12.070791 180-189
ATCTC 2998020 1.6626844 7.344194 100-109
CACCA 2720765 1.6565822 11.319741 230-239
GCAGA 3415305 1.6556232 13.540377 150-159
AACCA 2227160 1.6478089 12.683288 70-79
TGGTT 4288180 1.6393377 16.943323 10-14
AGCTC 3458650 1.626835 10.459123 140-149
GGGCG 6174665 1.6139442 49.79027 20-24
CACTG 3430210 1.613458 7.810129 230-239
TGCAG 3842365 1.6126517 12.726212 150-159
CAAAG 2440995 1.611489 30.482817 70-79
CGACT 3423125 1.6101254 10.51083 190-199
CTCTT 3348605 1.6078637 10.248385 200-209
ATAAT 1960850 1.6057823 5.587684 110-119
GGAGG 5055430 1.6057086 24.622446 30-34
TCGAC 3399055 1.5988038 11.131469 190-199
ACTGA 2792320 1.59601 8.454541 180-189
CTCCA 3023205 1.5936742 8.577168 240-247
ACGGA 3212710 1.5574123 10.008039 200-209
ATAAA 1634715 1.546231 6.170624 110-119
GTGGA 4102420 1.5363394 34.55783 25-29
GAGGG 4798275 1.5240307 10.682651 200-209
CAAAA 1689220 1.518711 19.875525 70-79
CTGGT 4151380 1.508497 7.812318 160-169
CCAGA 2773780 1.5069504 21.27429 60-69
CCTCG 3883675 1.5033022 9.242877 130-139
ACACG 2765830 1.5026314 10.33363 110-119
CTCGA 3191095 1.5009862 10.267211 190-199
GTCCC 3834410 1.4842328 15.50715 50-59
GCTCT 3635935 1.4806861 9.350924 130-139
TAGTT 2834525 1.4757204 9.465862 220-229
TGGAC 3515260 1.475365 11.967784 170-179
GGCCG 5027780 1.4728087 12.31271 120-129
TCCAC 2790985 1.4712601 11.171322 240-247
AATTG 2446605 1.4712204 9.2808895 170-179
GGGAG 4597395 1.4602273 7.525164 150-159
CTTGA 2942810 1.4562724 10.715284 200-209
AGCCG 3635675 1.4503859 10.989875 120-129
AAATA 1522115 1.4397259 5.3338675 6
TCAGA 2513785 1.4368074 8.523555 130-139
GCTCC 3708745 1.43559 5.2922854 220-229
CCATC 2702500 1.4246151 10.836217 240-247
CCACC 2836755 1.4213803 8.225745 230-239
TATGG 3210700 1.4177026 7.7283945 180-189
ATCGG 3342370 1.4028023 9.407666 190-199
TATAC 2069530 1.3946991 6.731927 110-119
ACTAT 2069015 1.3943522 7.3557444 230-239
ATGGG 3716470 1.3918025 47.92495 15-19
GAATT 2309840 1.3889793 10.426774 170-179
ACTCG 2924550 1.3756121 10.5499325 190-199
CGTAG 3275140 1.3745857 8.005116 210-219
GTGCT 3755350 1.3645906 8.146397 220-229
TGATA 2258805 1.3582903 8.336135 180-189
CAATA 1743975 1.3574979 7.961607 100-109
ACGTG 3226050 1.3539826 10.423603 170-179
GGCTC 3858420 1.3326557 8.490461 130-139
ACTGT 2687775 1.3300663 6.4361267 230-239
GCTCA 2800480 1.3172537 10.34415 140-149
AAAGT 1878010 1.304373 22.788034 70-79
ACCAT 2035390 1.3038062 9.42597 45-49
ATCCA 2026215 1.297929 17.658638 240-247
AAGTA 1866895 1.2966532 17.78078 70-79
TGCTC 3167230 1.2898122 5.3162594 220-229
TTGAG 2914625 1.286969 9.709469 200-209
CATGT 2596415 1.2848561 9.432755 70-79
CTGAT 2576715 1.2751074 7.064017 180-189
TTCCA 2266415 1.2569407 7.8019404 230-239
ACTTC 2262660 1.2548581 5.6868076 220-229
GAACC 2309635 1.2547878 9.793545 70-79
CAGAC 2288265 1.2431779 10.914399 60-69
ACTGG 2936725 1.232552 8.078704 160-169
GGAAT 2410680 1.2294618 8.493123 170-179
GTGTT 3215190 1.229142 7.0098624 160-169
TGTGC 3361490 1.2214727 6.0895348 220-229
GTCTA 2464350 1.2195027 8.6770315 180-189
GGAGT 3254905 1.2189484 7.01668 210-219
CCATG 2575525 1.2114421 9.463494 70-79
TATCA 1791825 1.207548 5.2985597 70-79
CCTCA 2282760 1.2033504 5.842959 130-139
GAGGC 3365205 1.1978848 21.764969 30-34
AGAGA 1992855 1.1739272 11.575839 35-39
GTCCA 2493920 1.173058 10.772842 70-79
CTGCG 3392190 1.1716253 6.3121123 210-219
TCTAC 2105640 1.1677759 8.222943 180-189
TCACG 2477610 1.1653863 8.656506 200-209
TGTAA 1933745 1.1628215 7.734335 230-239
AGTTC 2349350 1.162594 5.5868077 160-169
GCGTA 2757540 1.1573476 8.896048 210-219
ATCAT 1715625 1.1561953 5.5338173 90-99
GGTGG 4194605 1.1534789 34.573772 25-29
GGGCC 3913865 1.1465049 26.988924 15-19
GCATA 1973390 1.1279331 7.7029786 210-219
TAGAT 1868645 1.1236749 8.920645 240-247
GCGGT 3635925 1.1205428 7.642433 130-139
ACAAT 1439110 1.120193 9.2230625 100-109
CCCAA 1835765 1.1177353 10.861183 240-247
AGTTG 2527860 1.1161908 5.361376 220-229
AGAGC 2285330 1.10785 7.574391 140-149
CCAAT 1717845 1.1003969 12.367474 240-247
TGCCC 2838100 1.0985787 9.052504 60-69
TGGGC 3561945 1.0977432 28.675983 15-19
TATGT 2104785 1.0958006 7.392008 220-229
AGGTT 2467685 1.0896201 18.68622 10-14
ATAAC 1384930 1.0780196 7.67179 80-89
TATAA 1314610 1.0765624 7.6107354 240-247
CTCGG 3116315 1.076341 7.6695023 130-139
CGGCT 3100775 1.0709734 7.651457 130-139
TACTG 2141140 1.0595597 5.9237814 180-189
CGGTC 3059140 1.0565932 7.974252 130-139
CTGAA 1842435 1.0530831 12.989468 35-39
AGCTT 2105085 1.0417176 6.563725 150-159
GCTTC 2534240 1.0320355 6.166913 150-159
AGATC 1781155 1.0180571 5.653567 130-139
AAAAG 1264130 1.0141114 12.754353 70-79
TGGTC 2785965 1.0123428 7.5700145 50-59
ATACG 1745765 0.9978292 5.216924 120-129
CATCA 1544065 0.989079 5.630533 240-247
CGGAG 2776265 0.9882447 6.544731 210-219
GTGGT 3035520 0.98421526 6.6097064 50-59
GAAGA 1670275 0.9839057 16.640404 35-39
TCTGT 2242810 0.96091026 5.343046 210-219
ACCCA 1568200 0.9548242 12.933003 240-247
ACTAA 1217175 0.94744045 11.558497 240-247
GTCCG 2741765 0.94697547 5.1735806 120-129
GCCAC 2115730 0.94591844 5.009385 80-89
GAGCT 2250625 0.9445938 6.316427 140-149
GAGTT 2131700 0.9412641 5.167869 160-169
ATATC 1377055 0.9280259 5.357974 70-79
AAGAG 1567495 0.9233613 11.478229 35-39
CTACC 1747565 0.921224 7.002235 230-239
TCCGC 2354555 0.911407 5.085749 130-139
ACCGT 1935225 0.9102662 12.108689 45-49
TAGAC 1583825 0.9052689 5.0697336 110-119
CTTCC 1944265 0.88735485 5.3514066 230-239
CGATG 2088850 0.8766964 20.002253 15-19
TCCAG 1849205 0.8698052 5.377273 240-247
CCAGC 1924025 0.86020935 5.7473855 80-89
AATCC 1342765 0.86013263 11.362225 240-247
TGAAG 1681140 0.8573919 9.301017 35-39
TGCAT 1728700 0.8554606 6.3017087 210-219
AGCTG 1990865 0.8355718 6.0945725 30-34
TGCGT 2295555 0.8341413 6.628422 210-219
ATCAA 1061275 0.8260889 11.751161 70-79
AGCCA 1476735 0.8022866 7.44319 80-89
TAACC 1242595 0.7959669 7.1097517 80-89
CACAA 1068095 0.79025155 5.218687 100-109
GGTGC 2541080 0.7831264 5.3345804 220-229
TGAAC 1369340 0.78267545 5.1132426 190-199
CAATC 1209805 0.77496266 11.625057 240-247
ATACC 1196855 0.7666673 6.894068 240-247
CTAAT 1129310 0.7610655 9.244199 240-247
AGACC 1392145 0.75633013 5.387049 240-247
CCTTT 1571540 0.7545894 5.7831182 50-59
AAAGG 1271605 0.74906194 7.5286703 10-14
TCGAA 1304310 0.7455062 13.267308 70-79
CGGCG 2537060 0.74319166 5.2911077 130-139
TGAGA 1457065 0.7431122 6.0055227 240-247
GCGGC 2492335 0.73009014 5.451095 130-139
TAGGT 1649700 0.72843426 5.8614497 10-14
AGGAT 1404275 0.71618897 8.237496 10-14
CGAAC 1287020 0.69921744 9.722302 70-79
GGAAG 1609895 0.6963613 8.161142 30-34
GTCGA 1646365 0.6909842 9.283707 70-79
ACGTC 1442570 0.6785375 6.8968983 70-79
GGTCC 1935040 0.6683415 8.552018 50-59
AATGG 1282670 0.6541697 5.5486765 10-14
TGGAT 1464435 0.6466296 5.9367986 10-14
GTAGC 1521985 0.63878155 5.1834803 80-89
ACGAT 1113565 0.6364818 8.091818 10-14
GACCG 1564970 0.6243161 12.377871 45-49
AAGGT 1222375 0.6234188 6.6840796 10-14
AAAGC 942825 0.6224315 8.726705 70-79
TATGA 1016455 0.6112263 5.0491223 240-247
CATCC 1146415 0.60432947 7.410901 240-247
ATAGG 1151965 0.5875093 5.5516987 10-14
CGTCC 1418320 0.54900676 5.4469123 70-79
GAAGC 1113585 0.539828 7.6475034 30-34
TTGCC 1320390 0.53771114 6.0042934 60-69
TCAAG 931140 0.532213 5.6439667 70-79
AAGCT 896335 0.5123194 12.695559 30-34
CGGTT 1359815 0.49411923 10.120378 10-14
GACGA 1004695 0.48704183 5.871929 40-44
TAAAG 682355 0.4739301 5.098408 9
GGGAT 1245690 0.46650574 5.548348 10-14
AAGCA 689160 0.45496768 5.683925 70-79
TCGAT 829830 0.4106478 6.306882 10-14