FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_4_SL254631.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_4_SL254631.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 31493265
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 36854490 8.306277 56.76048 110-119
CACAG 47006725 7.1397266 40.744823 110-119
TCCCT 49495580 6.3087854 87.79911 30-34
CAGTA 39510465 6.282299 39.133068 60-69
ATACA 28856725 6.212669 29.282833 110-119
GGAGA 50886875 6.164563 92.06496 35-39
AGTAA 30249035 5.8160777 42.485294 110-119
GGTGA 55177880 5.7867436 94.10758 45-49
GTAAT 32574675 5.422148 36.554024 110-119
CCCTG 49222240 5.352336 52.69468 30-34
AGACG 38175755 5.1784134 102.53868 40-44
GGCCC 55784685 5.174872 58.463764 60-69
ACAGT 31148695 4.952749 33.852802 110-119
CCCCA 34946665 4.9150457 104.37152 60-69
GTAGT 39746555 4.8861423 24.988106 80-89
GTGAC 40953560 4.809206 84.51407 45-49
GAGAC 35204340 4.775351 134.0181 40-44
CCCTT 34877895 4.445592 70.26489 20-24
GTGTC 43609965 4.4334326 24.641459 120-129
TAGTA 25863960 4.30513 30.284317 80-89
TGACC 32716730 4.3019376 153.64006 45-49
ACCAG 28280500 4.29545 94.486885 45-49
AATAC 19824810 4.268155 29.709026 110-119
CGTGT 41888650 4.2584414 23.675575 120-129
GCACA 27414340 4.163891 34.95702 110-119
GACCA 26808110 4.0718126 106.90126 45-49
TGGTG 44293065 4.0214024 31.137733 25-29
CCCAG 31451500 3.9504879 85.53541 60-69
ACTCC 26759075 3.939833 88.37934 30-34
AGTAG 27642280 3.9252546 26.701988 70-79
CTCAG 29399040 3.865693 29.714342 140-149
CTTGG 37468055 3.8090396 40.580654 20-24
GACGG 37734445 3.7802658 113.2405 40-44
TGAGG 35872175 3.7620707 69.967026 35-39
CCACT 25326955 3.728977 26.7484 240-247
GCCCC 35548780 3.6925118 76.70702 60-69
AGGAG 29841985 3.6151328 91.56591 35-39
CTGAG 30766585 3.6129422 98.325775 35-39
CACTC 24390890 3.5911567 73.40426 25-29
ACGGT 30579775 3.5910048 93.32489 40-44
TGTCT 29973570 3.571825 21.770477 180-189
CAGGG 35614890 3.5679271 33.711872 50-59
CTCCC 29253855 3.561866 55.42557 30-34
ATGGT 28678740 3.5255477 25.769617 190-199
AGGCT 30011420 3.5242627 48.695957 30-34
GCCCT 32385660 3.5215573 37.430878 20-24
AGATA 18190075 3.4974632 18.913755 150-159
TCTTG 29293210 3.4907494 20.064865 170-179
TGTCC 30061225 3.421949 18.697147 120-129
GAGGA 28109860 3.4052987 128.70967 35-39
GATGG 31833335 3.3384998 36.29765 15-19
CCAGG 29105205 3.2648823 39.067673 50-59
CCGTG 33532495 3.256382 24.091375 120-129
CTCTC 25354345 3.2317054 13.529059 130-139
TCAGG 27502550 3.2296443 20.220348 140-149
CTTCA 20939330 3.2274117 17.490746 200-209
ACAGA 17224125 3.163523 14.254949 140-149
TTCCC 24359395 3.1048875 53.9404 50-59
TGGAG 29522510 3.0961537 34.181713 25-29
CCTGG 31804375 3.0885625 31.15291 50-59
TCGCA 23443960 3.082657 23.874763 100-109
CGCAC 24224495 3.042735 24.18456 100-109
ATGCA 19099085 3.0368197 73.58575 25-29
TCTCT 22700540 3.029008 16.998224 180-189
GGGTT 33306615 3.0239341 41.39563 50-59
AATAA 11386065 2.964265 14.257067 110-119
TCTCA 19060970 2.9378972 20.110867 130-139
GACTC 22314110 2.9340923 19.472254 190-199
GGATG 27859755 2.9217732 52.00983 20-24
TGGCC 29972545 2.910671 39.570667 60-69
GGTTC 28228740 2.869762 46.83425 50-59
TACAC 16068215 2.860794 20.60753 110-119
TTCTT 20256470 2.8295202 22.341064 170-179
TGCAC 21372185 2.8102381 94.55753 25-29
CAGAT 17669330 2.8094838 15.9717 150-159
GCCGT 28700275 2.7871192 23.068165 120-129
CGGTG 32014930 2.7765756 67.77681 40-44
CCAGT 21077945 2.7715485 39.25544 60-69
TCACA 15490620 2.7579587 12.065139 140-149
TCAAA 12784860 2.7524989 56.442947 70-79
GTTCC 23990145 2.730862 53.76047 50-59
CGGCC 29113040 2.700674 11.403345 200-209
ACCAC 15860025 2.6973543 15.626748 230-239
GGCTG 31092830 2.6966045 27.131866 30-34
AGGGT 25625125 2.6874177 49.44182 50-59
CCTGA 20384440 2.6803594 62.009872 30-34
AGATG 18662400 2.650095 17.24088 180-189
CGGAT 22220530 2.6093724 58.982925 20-24
GCACT 19693225 2.5894709 66.3983 25-29
AGGGA 21260150 2.5755076 9.06821 150-159
TGTTC 21326050 2.5413363 13.052918 150-159
GGTTG 27923605 2.5352063 54.678185 15-19
GAGAT 17832105 2.5321915 17.079916 180-189
AGTCA 15529940 2.4693134 28.740484 70-79
GTCTC 21428910 2.4393096 13.7220125 180-189
CTGGC 25114055 2.438857 34.780945 60-69
TTGTC 20391340 2.4299507 15.07945 170-179
TCATT 14978900 2.416887 17.622482 150-159
CATTT 14964650 2.4145877 18.817308 150-159
ACAGC 15818070 2.4025648 12.838674 120-129
TTCAT 14550475 2.3477595 17.004505 140-149
TCCTC 18307125 2.3334556 15.946362 130-139
CTGGA 19868120 2.3331275 15.850442 180-189
TCCAT 15111405 2.3291445 14.668334 70-79
ACTCT 15107365 2.3285217 12.614766 200-209
GTTCT 19500585 2.3238032 17.352884 160-169
CAGAG 17055505 2.313522 8.941222 140-149
GTTGG 25465365 2.3120208 42.47096 15-19
AATAT 10246275 2.3093088 13.394398 110-119
GATGC 19639710 2.3063052 70.36031 25-29
ATATA 10168810 2.2918499 11.091637 110-119
TTCAC 14785555 2.2789207 18.271423 200-209
TTGGT 21230480 2.2594304 44.88781 25-29
GTGAA 15877850 2.2546837 17.906965 190-199
TGTAG 18220510 2.2398922 8.712201 70-79
CACTA 12375585 2.2033563 15.479762 240-247
ATTTG 15265850 2.1998086 16.285982 150-159
GAATC 13807205 2.1953928 15.955794 190-199
TGAAT 13123510 2.184446 15.392786 190-199
CCTTG 19160270 2.1810644 46.06772 20-24
CTCTG 19079850 2.17191 15.78391 180-189
GAGTC 18322210 2.1515899 13.684913 210-219
GGCGG 28752370 2.1273224 34.33058 20-24
TCTGC 18641440 2.1220045 11.238769 210-219
TTGGA 17255115 2.1212134 17.058311 170-179
TAGTC 15307195 2.1070492 23.532728 70-79
TCGGC 21634520 2.100955 8.826197 190-199
GTTCA 15186500 2.0904355 15.748922 140-149
CAGCC 16585560 2.083241 11.610294 120-129
GATAC 13085175 2.0805874 13.317658 150-159
AATCG 13006125 2.0680182 14.208239 190-199
CCTTC 16144670 2.0578256 14.48419 200-209
ACTAC 11548590 2.0561175 11.769877 230-239
CTCGC 18891025 2.0541756 16.329142 100-109
AATAG 10678470 2.0531833 8.327846 240-247
GTCAA 12849455 2.0431073 39.407516 70-79
GCTGA 17356460 2.0381813 42.72287 30-34
GCGGA 20183175 2.021966 71.875984 20-24
ATAGA 10453710 2.0099678 9.816916 240-247
TCTCG 17607750 2.0043368 15.927645 100-109
TACAG 12598170 2.0031521 12.428871 160-169
CACGG 17855215 2.0029123 8.089955 200-209
ATATG 12022005 2.0010972 8.789589 180-189
TTTGC 16714095 1.9917488 12.247961 150-159
ACACA 9679845 1.9907408 13.147553 110-119
TTGCA 14406640 1.9830868 14.060268 150-159
TGGAA 13874670 1.9702286 10.205417 25-29
GTCCT 17123225 1.9491822 13.80559 130-139
ATTGT 13375130 1.927356 8.836063 170-179
GCTGT 18819115 1.9131697 10.22311 140-149
CTACT 12315600 1.8982227 7.4746895 230-239
GTTGT 17834025 1.8979667 7.084955 210-219
GATAT 11352505 1.8896571 8.632965 180-189
CAGGC 16818565 1.8866259 14.60148 140-149
AGTAT 11272650 1.8763651 12.1799 220-229
AGTCT 13626695 1.875727 13.942871 210-219
TCCCA 12702890 1.8702914 11.701052 240-247
GGTCA 15918155 1.8692803 9.579105 140-149
TTGGG 20345445 1.8471792 66.186485 15-19
CTGTT 15433235 1.8391141 12.803954 140-149
TACCA 10265905 1.8277476 11.614613 230-239
GTGGA 17268590 1.8110319 42.38584 25-29
ATAAT 7956215 1.7931744 6.479936 110-119
GTCAC 13625665 1.7916448 9.326075 140-149
TACTA 9582395 1.7859851 10.325314 240-247
CATAG 11199135 1.7807007 7.756872 210-219
TCTGG 17509065 1.7799889 13.02903 180-189
ATGTA 10678555 1.7774761 12.213579 70-79
GCAGA 13098135 1.776718 14.155611 150-159
GTCTG 17255720 1.7542336 9.344574 210-219
ATAGT 10496070 1.7471008 7.107083 220-229
ATAAA 6662430 1.734507 6.8474884 110-119
GAACT 10827735 1.7216468 10.4383545 160-169
CCAGA 11271640 1.7120194 26.405748 60-69
AAAAA 5691950 1.7117165 7.356421 7
GGAGG 19011225 1.7009124 29.7355 30-34
TGCAG 14471410 1.699388 13.21718 150-159
GTCCC 15617955 1.6982679 19.463243 50-59
TTGTA 11775370 1.6968306 9.249898 220-229
CAAAA 6812045 1.6940883 22.204126 70-79
GACGT 14335020 1.6833717 9.589419 170-179
AGAAC 9159855 1.6823735 10.76308 160-169
CACCA 9856280 1.6762823 11.642448 230-239
ACGGC 14860755 1.6670082 12.14292 120-129
ATGGG 15746190 1.6513711 58.632557 15-19
ACGGA 12162775 1.6498398 10.400373 200-209
TGGGG 21105925 1.6347407 39.573082 15-19
TCAGC 12418720 1.632943 10.380844 140-149
AACCA 7931395 1.6311574 12.33363 70-79
AAATA 6217755 1.6187396 5.7317734 6
TTCAG 11699520 1.6104496 9.787933 150-159
GGACG 16059765 1.6088796 7.7498293 170-179
CCCAC 11407080 1.6043397 10.805062 240-247
CAGCT 12138810 1.5961376 8.686582 150-159
GAGAA 9716055 1.5937172 10.733303 160-169
ATCTC 10333655 1.5927424 6.9428587 100-109
TGACT 11547335 1.5895013 11.258626 190-199
CGACT 12070570 1.5871645 9.301278 200-209
TCGAC 11946390 1.5708362 9.527534 190-199
CCTCG 14423225 1.568355 9.996327 130-139
AACTG 9773185 1.5539697 10.174815 160-169
CACTG 11734350 1.5429549 6.2462764 230-239
CTCTT 11517765 1.5368533 8.866198 200-209
CAAAG 8344260 1.5325747 28.001125 70-79
GGGGC 20665725 1.5290099 36.96104 15-19
AGCCG 13495990 1.5139154 11.223396 120-129
AATTG 9069520 1.5096476 9.91114 170-179
CTCCA 10162270 1.4962269 8.041677 240-247
ACTGA 9392315 1.4934101 7.0855093 180-189
TGGTT 13949925 1.4846057 14.794623 10-14
GCTCT 13018155 1.481891 9.26633 130-139
GGGCG 19898635 1.4722546 44.628227 20-24
CAATA 6837610 1.4720938 8.006012 100-109
CAGAC 9644895 1.4649373 14.0767765 60-69
TCAGA 9199125 1.4626923 8.22066 130-139
CTCGA 11066445 1.455132 8.6521 190-199
AGCTC 11049695 1.4529294 8.90381 140-149
GAATT 8652145 1.4401743 11.009529 170-179
GGCCG 17355585 1.4378427 11.83497 120-129
CCACC 10189440 1.4330857 8.354322 230-239
ATCGG 12178485 1.4301282 9.7396965 190-199
TCCAC 9629750 1.4178221 9.310826 240-247
CTGGT 13860120 1.4090335 6.611279 160-169
TGGAC 11960390 1.4045172 10.688978 170-179
GAGGC 13976730 1.4001995 26.226034 30-34
CCATC 9459625 1.392774 10.732638 240-247
CGTAG 11842420 1.3906637 8.011824 210-219
ACCAT 7741630 1.3783242 11.370368 45-49
GAGGG 15404550 1.3782274 8.568715 200-209
GGGCC 16592420 1.3746175 33.26551 15-19
GGTGG 17636075 1.3659866 42.682396 25-29
CATGT 9913680 1.3646271 11.856607 70-79
GTCCA 10352430 1.3612458 13.642976 70-79
ACTAT 7289670 1.3586628 7.3897595 230-239
GGCTC 13698315 1.3302603 8.395306 130-139
TGGGC 15334190 1.3298965 35.255024 15-19
GGGAG 14826395 1.3265004 6.3957567 150-159
CTTGA 9596330 1.3209435 9.0117445 200-209
AAGTA 6853620 1.3177673 17.472425 70-79
CCATG 9982440 1.3125955 12.009547 70-79
TTCCA 8413110 1.2967257 7.8577514 230-239
ACACG 8536080 1.2965225 8.653983 120-129
TATGG 10545315 1.2963614 6.487981 190-199
TAGTT 8977835 1.2937058 7.8634653 220-229
GGAGT 12320920 1.2921482 7.6177855 210-219
TATCA 6909360 1.2877797 6.3221087 70-79
TATAC 6901805 1.2863716 6.498924 110-119
AGAGA 7837285 1.2855439 14.192908 35-39
ACTCG 9762110 1.2836243 8.919446 190-199
AGAGT 8908795 1.2650651 5.082844 210-219
CCTCA 8582870 1.2636862 5.849822 130-139
ACGTG 10722990 1.2592084 9.021392 170-179
GTGTT 11825830 1.2585511 7.602287 160-169
ACTTC 8154340 1.2568412 5.6238685 220-229
GAACC 8267590 1.2557422 9.085612 70-79
AAAGT 6528890 1.2553302 20.848087 70-79
ACAAT 5764465 1.2410525 8.862832 100-109
TCACG 9321960 1.2257488 9.227019 200-209
GGAAT 8547180 1.2137152 8.821243 170-179
TGCCC 11145640 1.2119565 11.251947 60-69
ATCAT 6497245 1.2109691 5.174444 90-99
GCGTA 10281540 1.2073685 9.221981 210-219
CTGCG 12393775 1.2035749 6.5687337 210-219
GTGCT 11832440 1.2028975 6.7309675 220-229
TTGAG 9750390 1.1986393 8.154436 200-209
AGTTC 8693090 1.1966118 5.803648 160-169
GCTCA 8984060 1.181318 8.43861 140-149
TGATA 7074575 1.1775833 6.163038 180-189
CAGTG 9817945 1.1529284 5.0819626 160-169
AAAAG 5151725 1.1441914 14.527699 70-79
TGTGC 11179780 1.1365477 5.901892 220-229
GCATA 7061055 1.1227317 7.4005866 210-219
TATAA 4951400 1.1159482 8.170177 240-247
ACTGG 9478420 1.1130576 6.747548 160-169
TATGT 7689775 1.1080964 7.3807826 220-229
CTCGG 11390470 1.1061428 7.886613 130-139
CTGAT 7991355 1.1000173 5.596218 180-189
CGGCT 11320540 1.0993515 7.7337275 130-139
TGGTC 10728105 1.0906299 9.806378 50-59
GCGGT 12565460 1.0897713 6.805509 130-139
CTGAA 6826505 1.0854377 15.48315 35-39
GTCTA 7877815 1.0843884 7.348655 180-189
ATAAC 5000860 1.0766532 7.1559525 80-89
TTGGC 10586400 1.0762239 5.037268 170-179
GTGGT 11826375 1.073726 8.593076 50-59
CCCAA 6307190 1.0726796 9.035501 240-247
CCAAT 5965940 1.0621793 10.280604 240-247
ATCCA 5948215 1.0590236 14.309598 240-247
GAAGA 6416665 1.0525208 20.138441 35-39
CGATG 8948120 1.050784 24.80079 15-19
CATCA 5895175 1.0495803 6.5504103 240-247
ACCGT 7901045 1.0389119 15.620194 45-49
TCTAC 6700915 1.0328225 6.817445 180-189
CGGAG 10282220 1.0300806 7.18151 210-219
TAGAT 6169640 1.0269542 7.767797 240-247
AGGTT 8297305 1.020008 16.70375 10-14
CGGTC 10354305 1.0055193 6.7537036 130-139
CCATT 6523135 1.005421 5.389839 50-59
AAAAT 3827630 0.9964909 5.3984485 5
TAGAC 6185580 0.9835283 5.3620825 110-119
GAGTT 7927765 0.9745796 5.3147273 160-169
AAGAG 5894525 0.9668745 14.071429 35-39
ATATC 5172960 0.9641462 6.2631946 70-79
TCTGT 8079325 0.9627794 5.0564213 210-219
AGAGC 7042125 0.95524067 5.8521686 140-149
TGAAG 6680175 0.94859713 10.921772 35-39
ATCAA 4350860 0.93671244 14.190792 70-79
TGTAA 5581010 0.92897516 6.1669025 230-239
CCTTT 6758130 0.90175956 7.2325697 50-59
GCTTC 7906800 0.90005195 5.020789 150-159
CTACC 6065655 0.8930678 6.8082337 230-239
ACTAA 4144175 0.8922145 11.545489 240-247
CACAA 4323430 0.88914937 5.432888 110-119
AGCTT 6375660 0.87761533 5.317234 150-159
CTTCC 6792740 0.86581355 5.4557605 230-239
TGCGT 8485900 0.86268497 6.80144 210-219
AGCTG 7239800 0.8501749 8.143058 30-34
TGCAT 6078925 0.8367696 6.0346146 210-219
GAGCT 6874765 0.8073086 5.0293894 140-149
CCAGC 6375080 0.8007465 5.5879526 80-89
TAACC 4495350 0.80035466 6.516487 80-89
AGCCA 5203095 0.7902843 7.209344 80-89
ACCCA 4645865 0.79013395 10.615381 240-247
AAAGA 3529400 0.7838751 5.0225263 8
AAATG 4059685 0.7805685 5.245154 9
GGTCC 8015105 0.7783568 10.933693 50-59
ACGTC 5855750 0.7699752 8.922714 70-79
TGAAC 4816250 0.7658002 5.532644 190-199
GGAAG 6318425 0.7654298 11.5663595 30-34
TCGAA 4787580 0.76124156 13.0829935 70-79
CGGCG 9181425 0.7606453 5.0497923 130-139
AGGAT 5351210 0.75988156 9.504238 10-14
TGAGA 5260330 0.7469765 5.41848 240-247
GAAGT 5245530 0.74487484 5.435506 70-79
AAAGG 4531915 0.7433666 7.544216 10-14
AATCC 4173465 0.74304605 9.220321 240-247
GCGGC 8964585 0.742681 5.2140684 130-139
GTATG 6025335 0.7407093 5.113463 220-229
ACGAT 4656870 0.74045825 9.664908 10-14
GACCG 6494220 0.7284904 15.704504 45-49
CAATC 4081000 0.72658354 9.385963 240-247
TAGGT 5887715 0.72379124 5.5438247 10-14
CTAAT 3823980 0.7127208 9.24775 240-247
CTTTG 5951845 0.7092565 6.284241 50-59
GTCGA 5965400 0.70052123 8.688033 70-79
ATACC 3918405 0.69763505 5.720905 240-247
CGAAC 4544855 0.6903059 9.028655 70-79
AAAGC 3750265 0.6888042 9.532952 70-79
CGTGG 7840180 0.6799594 5.9265976 50-59
CGTCC 6174345 0.67138696 7.235685 70-79
TTTCG 5576415 0.66451806 5.169446 100-109
AATGG 4668295 0.66290647 5.382108 10-14
TGGAT 5382505 0.66168463 7.508728 10-14
TTGCC 5608220 0.6383985 7.420908 60-69
GAAGC 4700170 0.63756233 10.653242 30-34
GATGA 4285850 0.6085986 5.2582774 45-49
ATAGG 4199045 0.5962721 5.3188167 10-14
AAGGT 4169575 0.59208727 6.1939597 10-14
AAGCT 3718410 0.59123987 16.790953 30-34
GACGA 4163805 0.5648062 7.5212097 40-44
CATCC 3715155 0.5469954 6.393836 240-247
TCAAG 3307050 0.52583224 5.737354 70-79
AAGCA 2751095 0.5052885 6.2253847 70-79
GAAAA 2245135 0.49864155 5.111098 6
GGGAT 4723975 0.49542373 6.5731835 10-14
CGGTT 4724800 0.4803278 9.235609 10-14
TAAAG 2469770 0.4748705 5.337736 9
TCGAT 3247130 0.4469704 7.3254137 10-14
GCGAT 2861235 0.33599684 5.55959 10-14