FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_14_SL254624.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_14_SL254624.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5285139
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 5758550 7.6778517 53.282345 110-119
CACAG 8051060 7.0649996 37.589447 110-119
TCCCT 8408630 6.3672814 92.64381 30-34
CAGTA 6554575 6.1392336 41.04682 60-69
ATACA 4772160 5.9611635 29.943888 110-119
GGAGA 8280040 5.9565835 88.59787 35-39
GGTGA 8900435 5.65962 89.64261 45-49
AGTAA 4863095 5.50024 39.620785 110-119
CCCTG 8505575 5.463548 54.824272 30-34
GTAAT 5191595 5.1901627 34.858128 110-119
ACAGT 5403610 5.0612016 31.444092 110-119
GTAGT 6349865 4.7599044 24.45258 70-79
GAGAC 5937975 4.717917 121.4244 40-44
GGCCC 8653280 4.715135 54.992622 60-69
AGACG 5858385 4.6546803 93.59946 40-44
GTGAC 6597590 4.6334934 79.53912 45-49
AAAAA 2695375 4.5996337 29.38742 230-239
CCCCA 5692110 4.5685697 92.941925 60-69
ACTCC 5296840 4.5376854 95.74157 30-34
GTGTC 6882565 4.272527 24.491005 120-129
ACCAG 4851800 4.2575717 90.673805 45-49
GCACA 4800450 4.2125106 30.83506 110-119
CGTGT 6659835 4.1342616 23.657713 120-129
AATAC 3281995 4.099718 29.94173 110-119
CACTC 4725730 4.048428 81.24358 25-29
CCCTT 5274250 3.9938295 58.71644 20-24
TGACC 5115595 3.9679527 143.21536 45-49
TGGTG 6974090 3.919899 26.574148 25-29
TAGTA 3905170 3.9040923 24.456667 80-89
CTCAG 4969905 3.8549476 28.71362 140-149
CCACT 4491050 3.847383 26.557947 240-247
GACCA 4374320 3.8385713 100.65129 45-49
CCCAG 5274845 3.833271 77.92834 60-69
TGAGG 5997920 3.813965 68.85044 35-39
AGTAG 4379530 3.714069 26.782944 70-79
CTCCC 5178355 3.673754 57.64419 30-34
CAGGG 6107035 3.6382785 33.16619 50-59
AGGAG 4951020 3.5617175 88.15073 35-39
CTGAG 5064965 3.5571294 94.64575 35-39
CTTGG 5710250 3.5447824 35.568066 20-24
GACGG 5879850 3.5029325 106.944016 40-44
TGTCT 4783300 3.5004172 20.303663 170-179
ATGGT 4628020 3.469197 24.057564 190-199
TCTTG 4731130 3.4622393 18.78081 170-179
CTTCA 3763785 3.4415405 16.634075 200-209
ACGGT 4864300 3.4162018 89.35686 40-44
GCCCC 5636460 3.3920867 69.4481 60-69
AGATA 2970165 3.3593051 17.531734 150-159
ATGCA 3566165 3.3401892 81.13887 25-29
AGGCT 4683570 3.2892754 40.70003 30-34
GAGGA 4543080 3.2682493 122.89259 35-39
GGGTT 5801915 3.2610595 43.26802 50-59
CCTGG 5525175 3.2134387 31.207417 50-59
TGTCC 4685460 3.212432 18.961546 120-129
CCAGG 4858345 3.196694 37.797302 50-59
TTCCC 4221500 3.1966538 54.676018 50-59
TCAGG 4512420 3.1690767 19.6894 140-149
GGATG 4973280 3.162416 60.003345 20-24
GCCCT 4910420 3.1542037 30.470451 20-24
CTCTC 4126835 3.1249704 13.141053 100-109
CCAGT 4009680 3.1101413 40.190403 60-69
TGCAC 4001545 3.1038308 105.00582 25-29
GATGG 4870935 3.0973363 30.465765 15-19
CCGTG 5197640 3.0229445 23.1906 120-129
GGTTC 4839175 3.0040407 48.281567 50-59
GTTCC 4353615 2.9849133 54.37469 50-59
TCACA 2865380 2.964142 11.81631 130-139
ACAGA 2764830 2.9297245 13.449888 140-149
TGGAG 4577820 2.91095 29.902351 25-29
TCGCA 3744465 2.904425 23.470215 100-109
TCTCA 3169515 2.8981502 20.020563 130-139
TACAC 2759175 2.8542764 21.850784 110-119
GCACT 3663110 2.841321 74.48212 25-29
GGTTG 5039815 2.8327086 63.072273 15-19
TCTCT 3499175 2.8281667 14.824766 180-189
CGGAT 4023940 2.8260164 67.22419 20-24
TTCTT 3274340 2.8247087 20.932604 170-179
CGCAC 3880070 2.8196769 23.856693 100-109
GACTC 3632160 2.8173144 20.22714 190-199
AGGGT 4394955 2.7946696 50.142735 50-59
TGGCC 4803090 2.7934742 37.822323 60-69
GCCGT 4774585 2.7768955 22.06343 120-129
CAGAT 2947960 2.7611575 14.921446 150-159
AGTCA 2913825 2.7291858 28.57746 70-79
CCTGA 3512435 2.724449 64.181946 30-34
ACCAC 2766515 2.6812623 14.433018 230-239
CGGTG 5047905 2.658202 65.85474 40-44
GTTGG 4687410 2.6346338 49.128124 15-19
AGGGA 3648645 2.6248012 9.56728 150-159
GATGC 3639880 2.556291 78.25671 25-29
GGCTG 4819400 2.5378723 23.012085 30-34
CGGCC 4637380 2.5268884 10.595443 120-129
TCCAT 2729710 2.496 11.58266 70-79
TCAAA 1997090 2.4946733 51.12205 70-79
AATAA 1635270 2.4666345 11.200632 110-119
TGTTC 3338530 2.443135 12.970195 140-149
AGATG 2880770 2.4430425 16.501114 180-189
CTGGC 4163160 2.4212914 33.91161 60-69
GAGAT 2802735 2.376865 16.040329 180-189
GTCTC 3442785 2.3604324 12.064654 180-189
ACTCT 2534815 2.3177915 12.5648155 190-199
GTTCT 3158180 2.311155 18.434923 160-169
TCCTC 3039485 2.3015945 14.303855 130-139
GGCGG 5142645 2.2972362 38.8886 20-24
CAGAG 2866675 2.2776678 9.140341 140-149
TTGTC 3107735 2.2742393 14.989407 170-179
GCGGA 3809515 2.2695262 80.634445 20-24
TTCAT 2323070 2.267258 17.1173 140-149
TCATT 2318235 2.262539 15.691933 140-149
CATTT 2315815 2.2601771 16.681793 150-159
CTGGA 3213945 2.2571564 14.390241 180-189
TTCAC 2426800 2.2190244 16.695406 200-209
ACAGC 2495230 2.1896245 11.425542 110-119
TGTAG 2917155 2.1867204 8.684486 210-219
TTGGT 3262730 2.1618533 38.084984 25-29
TCTGC 3121305 2.1400201 10.793589 210-219
GTGAA 2522995 2.1396308 16.048265 190-199
CACTA 2053480 2.124258 13.803527 240-247
TTGGG 3775085 2.121847 76.93814 15-19
GATAT 2118885 2.1183002 9.559697 180-189
TAGTC 2556860 2.1168392 24.799152 70-79
ATATA 1575110 2.100088 9.69954 110-119
TTGGA 2787045 2.089189 16.264414 170-179
ATTTG 2362805 2.0879464 14.711863 150-159
TGAAT 2086030 2.0854545 13.962269 190-199
GAGTC 2960415 2.0791023 12.084711 210-219
CTCTG 3028800 2.0765972 14.37256 180-189
TCTCG 3027750 2.0758772 15.9896965 100-109
ACTAC 1992470 2.0611453 12.309351 230-239
GTTCA 2486830 2.058861 15.48038 140-149
GATAC 2187515 2.0488997 12.851604 150-159
CCTTC 2682965 2.0316265 13.148013 200-209
CTACT 2217540 2.0276804 8.375674 230-239
GAATC 2154195 2.017691 14.249337 190-199
GAACT 2149975 2.0137384 12.009836 160-169
AATAT 1504825 2.0063772 11.39289 110-119
TCGGC 3433380 1.9968517 8.44016 190-199
CAGCC 2746455 1.99587 10.380049 120-129
GTCAC 2542380 1.9720176 9.031328 130-139
ATATG 1961630 1.9610887 9.3718815 180-189
CTCGC 3034950 1.9494973 16.223726 100-109
GTCAA 2057860 1.9274604 38.143642 70-79
GTCTG 3102840 1.9261665 8.690036 210-219
CCTTG 2796805 1.9175376 39.698063 20-24
ACACA 1624950 1.9017153 12.777182 110-119
CACGG 2883320 1.897167 7.30539 200-209
GTTGT 2859935 1.8949652 7.583226 210-219
TACAG 2022075 1.893943 11.280112 160-169
AATAG 1668195 1.886756 8.16809 240-247
TGGGG 3949140 1.8829212 46.16605 15-19
TCAGC 2427325 1.8827744 11.225142 140-149
TTGCA 2269905 1.8792676 13.058938 150-159
TCCCA 2192145 1.8779621 10.849821 240-247
CTCCA 2187375 1.8738757 8.269314 240-247
ATAGA 1656670 1.8737208 9.399509 240-247
AATCG 1997295 1.8707331 13.076162 190-199
TACTA 1668375 1.842133 10.572077 230-239
TACCA 1777405 1.8386674 11.370996 230-239
GCTGT 2961750 1.8385816 9.776389 140-149
TTTGC 2511415 1.8378526 11.473776 150-159
GTCCT 2677795 1.8359421 12.17066 130-139
TTCAG 2207635 1.8277138 9.912514 150-159
AGTAT 1825650 1.8251462 11.213646 220-229
AGTCT 2200015 1.821405 12.467809 210-219
ATTGT 2057100 1.8178031 8.448513 170-179
CACCA 1860355 1.8030263 9.994971 230-239
GGTCA 2561995 1.7992914 9.435469 130-139
GCTGA 2539265 1.783328 35.11318 30-34
TGGAA 2095005 1.7766731 8.644698 170-179
CTGTT 2419525 1.7706074 12.32111 140-149
CATAG 1888940 1.7692444 7.652743 210-219
CAGGC 2684760 1.7665182 13.156331 140-149
ATCTC 1914695 1.7507643 6.528452 100-109
CCAGA 1965310 1.7246071 20.516363 60-69
GGGGC 3855355 1.7221996 42.210274 15-19
TTGTA 1935610 1.7104459 9.787337 210-219
ATAGT 1704490 1.7040197 6.674798 220-229
CAGCT 2179740 1.690733 8.938517 150-159
ATGTA 1690035 1.6895686 9.7523365 70-79
CCATC 1969440 1.6871756 11.014467 240-247
TCTGG 2711375 1.6831547 11.802886 180-189
CCCAC 2070395 1.6617291 10.050873 240-247
GGGCG 3672240 1.6404015 50.223022 20-24
TGCAG 2320410 1.629626 12.312407 150-159
GCAGA 2049455 1.6283594 12.95706 150-159
AGAAC 1524360 1.615273 10.232317 150-159
GGAGG 2993610 1.6147808 24.85628 30-34
GTGGA 2539270 1.6146742 36.78202 25-29
ACACG 1829545 1.60547 9.216814 110-119
TGGTT 2422285 1.6049825 17.312918 10-14
ATCGG 2270460 1.5945461 9.386633 190-199
TTCCA 1739725 1.5907747 7.271054 230-239
ACGGC 2417015 1.5903474 11.753217 120-129
TGACT 1918140 1.588039 11.798972 190-199
CACTG 2003745 1.5542213 6.502247 230-239
GAGAA 1619310 1.5536071 9.985687 160-169
CAAAG 1466150 1.5535913 26.655474 70-79
GACGT 2202195 1.5466033 9.537369 170-179
CTCTT 1908660 1.5426519 8.944517 200-209
ACTGA 1636765 1.5330487 7.25994 180-189
AACTG 1633370 1.5298688 9.97177 160-169
AGCTC 1968075 1.5265533 9.497754 140-149
GGACG 2548240 1.5181192 7.5222144 170-179
AACCA 1283730 1.5023779 11.152515 70-79
ATAAT 1119890 1.493145 5.121869 110-119
CGACT 1922675 1.4913385 9.373317 190-199
ATAAA 987410 1.4894052 6.7755804 8
CTGGT 2398835 1.4891378 7.0429716 160-169
GTCCC 2307845 1.4824421 15.392752 50-59
GCTCT 2159945 1.4808953 9.199381 130-139
CTTGA 1788510 1.4807177 9.346451 200-209
CCACC 1844785 1.4806513 7.596813 230-239
ATGGG 2302040 1.4638242 50.10247 15-19
AGTTC 1766755 1.4627067 6.4932036 160-169
TCGAC 1882160 1.4599127 9.786272 190-199
GAGGG 2704820 1.4590048 9.36477 200-209
ACGGA 1833735 1.4569628 9.538521 200-209
CAAAA 1027855 1.4525684 17.683023 70-79
CCTCG 2259405 1.4513268 8.704271 130-139
TCCAC 1690595 1.4482952 10.391061 240-247
TGGAC 2036505 1.4302393 10.378296 170-179
TCAGA 1509975 1.414293 8.40883 130-139
AAATA 934040 1.408902 5.5668526 6
TAGTT 1589945 1.404991 8.415409 220-229
CGTAG 1990885 1.3982003 8.096782 210-219
GGCCG 2814500 1.3885691 11.446472 120-129
AATTG 1383055 1.3826734 9.0913 170-179
AGCCG 2084865 1.3717993 9.994066 120-129
GGGAG 2533535 1.3666121 6.766745 150-159
GGAAT 1609640 1.3650583 8.477378 170-179
CTCGA 1750515 1.3578013 8.815781 190-199
ATCCA 1306405 1.3514333 15.766767 240-247
GGCTC 2311620 1.3444368 8.338076 130-139
CTGAA 1432005 1.3412637 12.17551 35-39
TATGG 1766890 1.3244735 6.877083 180-189
GAGGC 2219180 1.3220811 21.740452 30-34
CAGAC 1494635 1.3115783 10.485448 60-69
GAATT 1297680 1.2973219 10.069867 170-179
GTGTT 1949920 1.291998 7.493398 160-169
GTGCT 2081235 1.2919794 7.5962925 220-229
GAAGA 1345570 1.290974 15.8418255 35-39
ACTAT 1165430 1.2868074 6.584112 230-239
AGATC 1373305 1.2862833 6.010356 130-139
CAATA 1026620 1.2824066 6.7825704 100-109
CATGT 1540110 1.2750659 9.4333935 70-79
TATAC 1141810 1.2607273 6.0480833 110-119
TCCAG 1619780 1.2563956 5.667703 240-247
ACTTC 1361800 1.2452066 5.3095107 220-229
TGATA 1243635 1.2432919 7.0254846 180-189
AAAGT 1097980 1.2418334 20.288837 70-79
ACGTG 1762345 1.2376965 9.104066 170-179
ACCAT 1195115 1.2363074 8.961898 45-49
ACTCG 1592345 1.2351154 9.000125 190-199
AAGTA 1087880 1.23041 16.313631 70-79
GGTGG 2580030 1.2301395 36.945274 25-29
ACTGT 1485530 1.2298788 5.236128 230-239
GCTCA 1576780 1.2230424 9.049094 140-149
AGAGC 1538730 1.2225717 7.264441 140-149
TGTGC 1940420 1.2045649 6.075317 220-229
GGAGT 1892155 1.2031859 6.808489 210-219
CCATG 1547265 1.2001488 9.295968 70-79
AGAGA 1249560 1.1988596 11.173908 35-39
TTGAG 1599020 1.1986369 8.424369 200-209
GCGTA 1703075 1.1960711 9.024887 210-219
GGGCC 2417305 1.1926079 28.356617 15-19
CTGAT 1436080 1.1889389 6.1272764 180-189
CCTCA 1385755 1.1871457 5.623407 130-139
GTCCA 1521695 1.1803153 10.559809 70-79
AAGAG 1225580 1.1758525 11.128655 35-39
TGGGC 2201895 1.1595069 30.716497 15-19
TATCA 1049715 1.1590409 5.640717 70-79
TAGAT 1157785 1.1574656 7.752957 240-247
GAACC 1318510 1.1570244 8.324716 70-79
CTGCG 1956375 1.1378266 6.310975 210-219
ACTGG 1611385 1.1316772 6.9492493 160-169
CCCAA 1153805 1.1182494 9.661419 240-247
AGGTT 1477870 1.1078221 19.132704 10-14
TCACG 1425730 1.1058792 8.414574 200-209
TATGT 1249550 1.1041932 7.204617 220-229
GTCTA 1325380 1.0972897 7.1238794 170-179
ATATC 993050 1.0964743 5.5351934 70-79
GCATA 1169590 1.095477 6.984394 210-219
TGCCC 1687045 1.0836718 8.883792 60-69
ACCCA 1116510 1.0821036 11.422814 240-247
AAAAG 828415 1.0599992 11.920076 70-79
CGGCT 1817010 1.0567719 7.3034573 130-139
GCGGT 2003315 1.0549358 6.83485 130-139
CCAAT 1012755 1.0476619 10.70648 240-247
TGAAC 1116895 1.0461211 5.438605 190-199
ACAAT 833860 1.0416197 7.6866975 100-109
CTCGG 1786965 1.0392978 7.2639494 130-139
TCTAC 1131635 1.0347476 6.7697725 180-189
TGGTC 1632265 1.0132699 7.8775845 50-59
GTGGT 1798115 1.0106593 6.998487 50-59
TCTGT 1367435 1.0006883 5.031136 210-219
ATAAC 797665 0.9964066 6.4075866 80-89
AGACC 1131735 0.9931248 5.6278353 240-247
TACTG 1198510 0.99225324 5.0741076 180-189
TATAA 738365 0.9844591 6.652552 240-247
CCAGC 1349515 0.98070306 5.7042193 80-89
GCTTC 1420000 0.9735763 5.3762827 150-159
CGGAG 1621575 0.9660566 6.3215675 210-219
AGCTT 1166395 0.96566504 5.699966 150-159
CGGTC 1645025 0.95674556 6.8583055 130-139
CATCA 920390 0.95211345 5.3582573 230-239
TGTAA 944665 0.9444043 6.547134 230-239
GTATG 1258225 0.9431745 5.236496 220-229
ACTAA 751915 0.9392578 11.256967 240-247
GAGTT 1247205 0.93491393 5.2575064 160-169
GGAAG 1287990 0.926568 8.579581 30-34
CGATG 1272705 0.8938219 20.095564 15-19
ACGTC 1149095 0.89130485 6.9348383 70-79
ACCGT 1143795 0.887194 12.07438 45-49
CTACC 1032565 0.88457555 6.50432 230-239
TGAAG 1037720 0.88004047 8.598695 35-39
GAGCT 1237060 0.86878836 5.5063424 140-149
AATCC 838235 0.8671267 10.061808 240-247
TGCAT 1034415 0.85639805 5.7695866 210-219
TGCGT 1358725 0.84346306 6.8967147 210-219
AGCTG 1188975 0.8350182 6.5119214 30-34
ATCAA 660930 0.8256034 11.739936 70-79
AGCCA 904190 0.7934485 6.9909735 80-89
AAAGG 826055 0.7925381 7.2373276 10-14
CCTTT 979185 0.79141474 5.884435 50-59
GACCG 1190095 0.78305864 12.276994 45-49
TGAGA 903440 0.76616406 5.8998995 240-247
TGGAT 1006950 0.75481695 6.584769 10-14
TAACC 718645 0.74341476 6.160629 80-89
CTAAT 661875 0.730808 9.0523 240-247
CAATC 695905 0.71989095 9.976692 240-247
TAGGT 953990 0.71511775 6.3245025 10-14
AGGAT 834335 0.7075594 7.978325 10-14
ATACC 672725 0.695912 5.808918 240-247
TCGAA 728190 0.68204707 11.878737 70-79
GGTCC 1158670 0.67388177 8.705011 50-59
AAGGT 793090 0.6725815 6.9083915 10-14
AATGG 771465 0.6542424 5.4537506 10-14
GTCGA 925860 0.6502323 8.256383 70-79
CATCC 741475 0.6352052 7.247188 240-247
CGAAC 714860 0.6273069 8.3362 70-79
ACGAT 669535 0.6271088 8.359036 10-14
CTTTG 853840 0.62483984 5.1669016 50-59
ATAGG 710620 0.60264266 5.7806573 10-14
AAAGC 552920 0.5858962 7.7871556 70-79
GAAGC 705570 0.5605987 7.691646 30-34
CGTCC 865015 0.5556416 5.5344605 70-79
TTGCC 809215 0.5548117 6.008236 60-69
TCAAG 573105 0.5367893 5.3908367 70-79
AAGCT 542995 0.50858724 12.736564 30-34
CGGTT 812480 0.5043676 10.565673 10-14
GACGA 615225 0.48881653 5.786701 40-44
GGGAT 750435 0.47718757 5.674958 10-14
TAAAG 411925 0.46589392 5.939266 9
AAGCA 433390 0.4592374 5.086242 70-79
TCGAT 510000 0.4222319 6.632853 10-14