FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_12_SL254622.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_12_SL254622.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8288501
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 9768410 8.486246 59.81774 110-119
CACAG 12678475 7.3082256 42.35085 110-119
TCCCT 13817745 6.6953936 99.89038 30-34
CAGTA 10945865 6.6377106 44.787388 60-69
ATACA 7833170 6.4685364 33.77162 110-119
GGAGA 13642825 6.258698 93.745125 35-39
AGTAA 8067520 5.943275 44.64478 110-119
GGTGA 14738620 5.8552055 94.44994 45-49
CCCTG 14015140 5.75878 59.7182 30-34
GTAAT 8931070 5.6976395 38.495983 110-119
ACAGT 8308730 5.0385184 35.017418 110-119
AGACG 9665280 4.970233 101.8222 40-44
GTAGT 10591525 4.9619193 25.625435 70-79
GTGAC 11131140 4.956871 85.344406 45-49
GGCCC 13967600 4.8668613 59.358364 60-69
CCCCA 9073925 4.8261986 102.99087 60-69
GAGAC 9328140 4.796863 132.04668 40-44
ACCAG 7892920 4.5496984 100.756454 45-49
AATAC 5509010 4.5492735 33.738373 110-119
GTGTC 11584740 4.467452 25.742144 120-129
ACTCC 7809455 4.3697243 100.05578 30-34
CGTGT 11277730 4.34906 24.806477 120-129
TGACC 8568415 4.2771173 154.6508 45-49
GCACA 7407860 4.2700963 34.762714 110-119
GACCA 7272700 4.1921864 111.54566 45-49
TAGTA 6335235 4.0416083 25.605295 80-89
CCACT 7155455 4.003783 28.840782 240-247
CACTC 7147940 3.9995782 83.59788 25-29
CTCAG 7987075 3.9869282 30.905323 140-149
CCCAG 8340080 3.9572873 84.82471 60-69
CCCTT 8126035 3.9374733 57.678074 20-24
AGTAG 7275765 3.9360826 29.219675 70-79
TGAGG 9863550 3.9184885 70.96284 35-39
CTCCC 8503255 3.9165246 62.67543 30-34
TGGTG 11354060 3.9060895 25.11931 25-29
CAGGG 10087780 3.8094096 35.917587 50-59
GACGG 9856175 3.7219496 113.860725 40-44
AGGAG 8023255 3.6806986 93.27986 35-39
CTGAG 8240405 3.6695812 100.07 35-39
TCTTG 7997245 3.636805 20.229187 170-179
ACGGT 8121835 3.6167808 94.71069 40-44
ATGGT 7697165 3.6059692 25.996004 190-199
TGTCT 7908350 3.5963788 21.642908 180-189
GCCCC 9136790 3.5686457 75.43101 60-69
CTTGG 9226150 3.5579035 33.432602 20-24
AGATA 4729745 3.484364 18.310265 150-159
GAGGA 7490875 3.4364674 130.78574 35-39
CCAGG 8093180 3.4258149 41.62872 50-59
ATGCA 5646105 3.4238694 84.26648 25-29
GGGTT 9715755 3.3424704 45.079285 50-59
TTCCC 6895105 3.3410263 58.734978 50-59
CTTCA 5670640 3.3380177 16.9236 200-209
AGGCT 7450965 3.3180315 40.94589 30-34
TGTCC 7673065 3.3168447 19.739996 120-129
TCAGG 7309075 3.2548459 20.332811 140-149
CCTGG 8827185 3.2357328 33.01025 50-59
CTCTC 6623635 3.2094848 13.942945 100-109
GCCCT 7769535 3.1924791 30.093296 20-24
ACAGA 4540065 3.1792493 16.007225 140-149
CCGTG 8644725 3.1688492 24.975098 120-129
TCGCA 6271785 3.1307023 25.672411 100-109
GGTTC 8107705 3.1265948 50.981506 50-59
TGCAC 6242655 3.1161613 107.53526 25-29
GACTC 6200385 3.0950618 23.29295 190-199
TACAC 4546250 3.0903249 24.27463 110-119
CGCAC 6509300 3.0886 26.327475 100-109
GGATG 7652265 3.0400121 58.874527 20-24
CCAGT 6060565 3.0252674 43.592342 60-69
GATGG 7590760 3.015578 29.307333 15-19
CCTGA 5943890 2.9670265 70.338554 30-34
TGGCC 8056405 2.9531922 40.646805 60-69
TCTCA 4980105 2.9315348 20.87054 130-139
GTTCC 6690025 2.8919048 58.30242 50-59
GCACT 5787295 2.8888586 75.66708 25-29
TCTCT 5656740 2.8835566 15.68111 180-189
TTCTT 5372565 2.8811588 22.396606 170-179
AGGGT 7238190 2.8755128 53.292877 50-59
TGGAG 7196240 2.8588474 28.626722 25-29
GGTTG 8242615 2.8356721 61.684605 15-19
CGGAT 6364755 2.8343253 67.02487 20-24
TCACA 4140045 2.8142061 13.655301 130-139
GCCGT 7654990 2.8060477 23.662199 120-129
AGGGA 6058480 2.7793508 10.625711 200-209
TCAAA 3349975 2.7663682 57.702442 70-79
CGGTG 8398820 2.7465403 68.409096 40-44
ACCAC 4197020 2.7118685 15.892247 230-239
CAGAT 4471535 2.711595 15.390367 150-159
AGTCA 4418855 2.679649 31.592346 70-79
CGGCC 7684510 2.6775854 11.687318 120-129
AATAA 2621395 2.6297746 12.543015 110-119
GTTGG 7591335 2.6116152 48.258446 15-19
GATGC 5741360 2.5567176 79.46643 25-29
CTGGC 6972605 2.5559094 36.774593 60-69
GGCTG 7626535 2.493992 22.691536 30-34
AGATG 4608785 2.4932857 16.794079 180-189
TGTTC 5388955 2.450666 12.807906 140-149
ACTCT 4138230 2.4359658 14.094782 200-209
GAGAT 4430805 2.397001 16.346779 180-189
GTTCT 5245780 2.385556 19.115002 160-169
CAGAG 4588670 2.3596582 10.439234 140-149
ACAGC 4065645 2.343551 12.562878 120-129
GTCTC 5407310 2.337424 12.589534 180-189
TCATT 3745255 2.3193228 16.191326 150-159
TTCAT 3720645 2.3040826 17.07427 140-149
CATTT 3706405 2.2952642 17.483128 150-159
TGTAG 4862580 2.2780223 9.6260195 210-219
ATATA 2620695 2.2767127 10.644883 110-119
GGCGG 8202995 2.2747536 38.647457 20-24
CACTA 3338185 2.2691398 15.3306465 240-247
TAGTC 4315660 2.2663195 26.973894 70-79
TCCTC 4671905 2.2637734 14.975974 130-139
TTGTC 4977015 2.2633336 14.756191 170-179
CTGGA 5075805 2.2603357 15.115379 180-189
GCGGA 5936685 2.2418475 81.10568 20-24
TCCAT 3757045 2.2115812 11.4950075 70-79
CTACT 3733540 2.197745 8.7861805 230-239
ACTAC 3228730 2.1947374 13.027682 230-239
TTCAC 3711320 2.1846652 17.365545 200-209
GTGAA 3994640 2.161042 17.081099 190-199
TTGGA 4607135 2.1583514 17.86032 170-179
AATAT 2473920 2.1492026 12.518833 110-119
GTCAA 3528775 2.1398935 42.33318 70-79
TTGGT 5264205 2.1356483 36.219883 25-29
ATATG 3339425 2.13041 11.150579 180-189
GATAT 3333465 2.1266077 11.014415 180-189
ATTTG 3803870 2.1014683 15.294434 150-159
TGAAT 3293985 2.101421 14.768602 190-199
CTCGC 5112280 2.1006207 17.689585 100-109
GAATC 3425645 2.0773544 15.149513 190-199
AATAG 2818975 2.0767157 9.39014 240-247
TCTGC 4802165 2.0758379 10.856879 210-219
GATAC 3416995 2.0721087 13.420174 150-159
CAGCC 4366155 2.0716982 11.411963 120-129
TCTCG 4791175 2.0710871 17.314865 100-109
GAGTC 4642420 2.0673425 12.7416525 210-219
CTCTG 4755080 2.055484 14.900278 180-189
TTGGG 5964150 2.0518215 75.023766 15-19
CACGG 4818455 2.0396352 7.5455704 200-209
GTTCA 3861555 2.027851 15.437108 140-149
ATAGA 2752475 2.0277257 11.223658 240-247
TCGGC 5524905 2.025234 8.604239 190-199
TCCCA 3582205 2.0043967 11.833339 240-247
CCTTC 4131690 2.0020123 13.735827 200-209
ACACA 2530880 1.986629 14.130508 110-119
AATCG 3269155 1.9824567 13.814128 190-199
TACAG 3239975 1.9647616 12.042232 160-169
AAAAA 1686480 1.9537164 7.4594045 230-239
GGTCA 4375210 1.9483498 10.799702 130-139
GTTGT 4796725 1.945995 8.473509 210-219
TACTA 2711625 1.939116 11.12738 230-239
GAACT 3180355 1.9286072 12.282164 160-169
GTCAC 3849140 1.9213846 10.371478 130-139
TTGTA 3454760 1.9086006 11.372503 210-219
CCTTG 4389770 1.8975713 37.83227 20-24
TACCA 2786325 1.8940115 12.055924 230-239
TTGCA 3584265 1.8822358 13.382359 150-159
AGTAT 2948200 1.880825 12.325699 220-229
GTCCT 4328915 1.8712653 12.752856 130-139
GCTGT 4819060 1.8583864 9.927446 140-149
AGAAC 2642160 1.8502126 12.317707 160-169
ATTGT 3336190 1.8430961 8.397077 170-179
CATAG 3033815 1.8397435 8.276913 210-219
TGGAA 3373580 1.8250576 9.363373 170-179
AGTCT 3474600 1.8246464 13.234732 210-219
TCAGC 3644790 1.819379 12.480156 140-149
TTTGC 4000470 1.8192427 11.668776 150-159
TGGGG 6202095 1.8093538 44.73688 15-19
GCTGA 4062655 1.8091642 35.217396 30-34
CAGGC 4267000 1.8062065 13.931084 140-149
CTGTT 3938115 1.790886 12.452299 140-149
CAGCT 3549675 1.7719002 10.254017 150-159
ATAGT 2737150 1.7461843 7.217286 220-229
GTCTG 4520015 1.7430646 8.995034 210-219
CCCAC 3258235 1.7329754 11.419084 240-247
GAGAA 2763635 1.726476 12.222347 160-169
ACGGC 4063890 1.7202307 13.148059 120-129
TGACT 3273770 1.7191828 13.387565 190-199
TTCAG 3267210 1.7157379 11.226367 150-159
AACTG 2817605 1.708631 11.90732 160-169
ATGTA 2671695 1.7044269 9.974704 70-79
GGGGC 6061290 1.6808422 41.425034 15-19
TCTGG 4332835 1.6708821 12.13257 180-189
GGACG 4424245 1.6707107 8.776641 170-179
GACGT 3745800 1.6680636 11.019625 170-179
ATCTC 2825090 1.6629871 7.336519 100-109
GCAGA 3216145 1.6538568 13.559572 150-159
CACCA 2554740 1.6507233 11.328452 230-239
AACCA 2097275 1.6462679 12.729196 70-79
TGGTT 4037630 1.6380359 16.944164 10-14
AGCTC 3251950 1.623284 10.421141 140-149
CGACT 3235390 1.6150178 10.552856 190-199
CACTG 3233400 1.6140244 7.8193827 230-239
GGGCG 5818240 1.6134427 49.812824 20-24
TGCAG 3622290 1.6130624 12.765238 150-159
ATAAT 1853040 1.6098169 5.5907774 100-109
CTCTT 3155695 1.608634 10.282082 200-209
CAAAG 2294960 1.6070806 30.411379 70-79
GGAGG 4766845 1.6058702 24.61688 30-34
TCGAC 3212100 1.6033918 11.173173 190-199
ACTGA 2633690 1.5971029 8.510571 180-189
CTCCA 2845430 1.5921397 8.555072 240-247
ACGGA 3027355 1.5567744 10.050077 200-209
ATAAA 1543110 1.5480427 6.1941915 110-119
GTGGA 3870660 1.5376955 34.724113 25-29
CAAAA 1598175 1.5240074 19.931908 70-79
GAGGG 4523760 1.523979 10.730768 200-209
CTGGT 3916290 1.5102489 7.835012 160-169
CCAGA 2612515 1.5059263 21.333956 60-69
CTCGA 3012360 1.503687 10.290952 190-199
CCTCG 3659325 1.5036061 9.252186 130-139
ACACG 2606615 1.5025256 10.360712 110-119
GTCCC 3612425 1.4843348 15.53566 50-59
GCTCT 3429630 1.4825304 9.384554 130-139
GGCCG 4750070 1.4765376 12.31981 120-129
TGGAC 3314975 1.4762104 11.968611 170-179
TAGTT 2667520 1.4736857 9.507191 220-229
AATTG 2304845 1.4703921 9.324555 170-179
TCCAC 2627045 1.469944 11.181123 240-247
GGGAG 4330865 1.4589958 7.51021 150-159
CTTGA 2770710 1.4550066 10.746875 200-209
AGCCG 3430455 1.4520997 11.025403 120-129
AAATA 1437120 1.4417139 5.423821 6
TCAGA 2373650 1.4394112 8.522076 130-139
GCTCC 3502975 1.4393623 5.305825 220-229
CCATC 2549500 1.4265543 10.836746 240-247
TATGG 3032955 1.4208795 7.751076 180-189
CCACC 2661475 1.4155735 8.213227 230-239
ATCGG 3153210 1.4041739 9.461214 190-199
ACTAT 1951835 1.3957808 7.412983 230-239
TATAC 1949455 1.3940789 6.7325726 110-119
ATGGG 3505770 1.3927358 48.06522 15-19
GAATT 2174415 1.3871835 10.450339 170-179
ACTCG 2757635 1.3765355 10.563648 190-199
CGTAG 3084275 1.373476 8.00353 210-219
GTGCT 3538055 1.3643892 8.1485405 220-229
TGATA 2129560 1.3585678 8.368036 180-189
CAATA 1644865 1.3583093 7.96569 100-109
ACGTG 3039730 1.3536395 10.436326 170-179
GGCTC 3636460 1.3329972 8.487977 130-139
ACTGT 2533925 1.3306618 6.452476 230-239
GCTCA 2631925 1.3137846 10.354256 140-149
ACCAT 1922030 1.3065047 9.425214 45-49
AAAGT 1762700 1.2985666 22.725185 70-79
AAGTA 1757070 1.2944189 17.749735 70-79
ATCCA 1902225 1.2930423 17.63871 240-247
TGCTC 2988385 1.2917926 5.3232636 220-229
CATGT 2446830 1.2849249 9.446351 70-79
TTGAG 2742430 1.2847743 9.742056 200-209
CTGAT 2427165 1.2745979 7.0874 180-189
GAACC 2178805 1.2559239 9.778215 70-79
TTCCA 2132240 1.2551414 7.81569 230-239
ACTTC 2129245 1.2533785 5.6650376 220-229
CAGAC 2152080 1.2405188 10.913677 60-69
ACTGG 2766980 1.2321796 8.081769 160-169
GTGTT 3030910 1.2296172 7.028459 160-169
GGAAT 2266365 1.2260704 8.532138 170-179
TGTGC 3162590 1.2195975 6.0968065 220-229
GTCTA 2322380 1.2195711 8.694463 180-189
GGAGT 3069865 1.219564 7.016594 210-219
TATCA 1695065 1.2121615 5.2968917 70-79
CCATG 2427100 1.2115415 9.491265 70-79
CCTCA 2147495 1.2016153 5.8369927 130-139
GAGGC 3171885 1.1977868 21.787313 30-34
GTCCA 2356920 1.1765095 10.802853 70-79
CTGCG 3198350 1.1724015 6.319377 210-219
AGAGA 1876040 1.1719847 11.522495 35-39
TCACG 2335865 1.1659995 8.690382 200-209
TCTAC 1977960 1.1643244 8.210232 180-189
AGTTC 2216985 1.1642243 5.623755 160-169
TGTAA 1819820 1.160967 7.714365 230-239
GCGTA 2599100 1.15742 8.915334 210-219
ATCAT 1618415 1.1573482 5.5424123 90-99
GGTGG 3949275 1.1521326 34.698982 25-29
GGGCC 3690095 1.1470492 27.136034 15-19
GCATA 1862175 1.1292464 7.7429013 210-219
TAGAT 1769145 1.1286386 8.948321 240-247
GCGGT 3431195 1.1220524 7.639401 130-139
ACAAT 1357190 1.1207508 9.19857 100-109
CCCAA 1729200 1.1173078 10.823078 240-247
AGTTG 2378310 1.114191 5.372588 220-229
AGAGC 2147070 1.1041002 7.526742 140-149
TGGGC 3360190 1.0988326 28.801638 15-19
TATGT 1986420 1.0974084 7.40107 220-229
CCAAT 1612365 1.0960093 12.331085 240-247
TGCCC 2653350 1.0902537 8.837151 60-69
AGGTT 2324115 1.0888019 18.692928 10-14
ATAAC 1310585 1.0822651 7.704198 80-89
CTCGG 2940550 1.0779012 7.6668553 130-139
TATAA 1236635 1.0743191 7.565403 240-247
CGGCT 2923825 1.0717704 7.672696 130-139
TACTG 2015250 1.0582855 5.9390526 180-189
CGGTC 2880315 1.0558213 7.98451 130-139
CTGAA 1734185 1.0516316 12.919075 35-39
AGCTT 1979720 1.0396273 6.5408726 150-159
GCTTC 2379770 1.0287061 6.1485686 150-159
AAAAG 1196420 1.0178031 12.774775 70-79
AGATC 1677285 1.0171268 5.583514 130-139
TGGTC 2620035 1.0103706 7.586289 50-59
ATACG 1643435 0.99659973 5.236744 120-129
CGGAG 2609640 0.9854683 6.549117 210-219
CATCA 1449010 0.98496825 5.555053 240-247
GAAGA 1567465 0.9792142 16.538546 35-39
GTGGT 2843855 0.97835946 6.6324096 50-59
TCTGT 2114165 0.9614318 5.3391438 210-219
ACCCA 1470705 0.95028347 12.887054 240-247
GTCCG 2585335 0.94769204 5.2026095 120-129
GCCAC 1991670 0.9450281 5.0103855 80-89
ACTAA 1142075 0.9431115 11.503898 240-247
GAGTT 2009970 0.9416311 5.194715 160-169
GAGCT 2111910 0.94046664 6.287055 140-149
ATATC 1298495 0.92856944 5.374328 70-79
AAGAG 1470965 0.9189296 11.416359 35-39
CTACC 1639130 0.9171633 6.9713335 230-239
TCCGC 2221655 0.91287154 5.055957 130-139
ACCGT 1821260 0.90912294 12.175265 45-49
TAGAC 1495950 0.90716296 5.112641 110-119
CTTCC 1822630 0.8831562 5.3511863 230-239
CGATG 1971000 0.8777172 20.021757 15-19
TCCAG 1731805 0.86446947 5.3762445 240-247
CCAGC 1808975 0.85834116 5.769311 80-89
AATCC 1259485 0.8561381 11.347775 240-247
TGCAT 1629425 0.85567385 6.3400245 210-219
TGAAG 1573605 0.8512973 9.244507 35-39
AGCTG 1879240 0.83685505 6.1393266 30-34
TGCGT 2164205 0.8345879 6.639431 210-219
ATCAA 999225 0.8251477 11.776554 70-79
AGCCA 1386905 0.7994506 7.4670815 80-89
TAACC 1166380 0.79284984 7.085273 80-89
CACAA 1006630 0.79016006 5.210144 100-109
GGTGC 2396845 0.7838044 5.337321 220-229
TGAAC 1286055 0.7798799 5.13267 190-199
CAATC 1136300 0.7724028 11.635287 240-247
ATACC 1125530 0.76508194 6.896913 240-247
CTAAT 1062665 0.7599246 9.213655 240-247
AGACC 1308100 0.7540253 5.3925295 240-247
CCTTT 1477820 0.7533274 5.809911 50-59
AAAGG 1202730 0.75135994 7.5527396 10-14
TCGAA 1230550 0.746221 13.253677 70-79
TGAGA 1376080 0.74443924 6.0342727 240-247
CGGCG 2384660 0.7412607 5.2822 130-139
TAGGT 1556225 0.7290606 5.8683133 10-14
GCGGC 2344500 0.72877717 5.426944 130-139
AGGAT 1331615 0.7203843 8.2836485 10-14
CGAAC 1218270 0.7022447 9.708607 70-79
GGAAG 1521890 0.6981728 8.166913 30-34
GTCGA 1552315 0.69127023 9.253461 70-79
ACGTC 1355845 0.67680055 6.92997 70-79
GGTCC 1820280 0.66725004 8.559649 50-59
AATGG 1210540 0.65488446 5.5940337 10-14
TGGAT 1381835 0.6473623 5.993944 10-14
ACGAT 1050710 0.63716376 8.135463 10-14
GTAGC 1429910 0.63676137 5.153109 80-89
GACCG 1473280 0.6236343 12.42596 45-49
AAGGT 1151010 0.62267965 6.7244363 10-14
AAAGC 888720 0.62233967 8.741648 70-79
TATGA 953295 0.60816133 5.0453897 240-247
CATCC 1081690 0.60525185 7.478238 240-247
CTTTG 1291875 0.5874895 5.015232 50-59
ATAGG 1083325 0.586063 5.552222 10-14
CGTCC 1329795 0.54640895 5.4637628 70-79
GAAGC 1052145 0.5410506 7.6402187 30-34
TTGCC 1225460 0.5297311 5.8062906 60-69
TCAAG 873525 0.52971655 5.611818 70-79
AAGCT 847275 0.51379824 12.741384 30-34
CGGTT 1283090 0.49480125 10.263661 10-14
GACGA 945700 0.4863128 5.898632 40-44
TAAAG 638645 0.4704845 5.145366 9
GGGAT 1166530 0.4634269 5.5061364 10-14
AAGCA 649765 0.4550078 5.6721582 70-79
TCGAT 782105 0.4107135 6.322706 10-14