FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_8_SL254635.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_8_SL254635.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9285764
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10666850 8.261849 58.195618 110-119
CACAG 13998720 7.1675344 41.245945 110-119
TCCCT 15174195 6.5176687 95.29127 30-34
CAGTA 12037850 6.5073476 43.36826 60-69
ATACA 8623640 6.3264213 32.169216 110-119
GGAGA 15033300 6.1917577 92.70629 35-39
AGTAA 8862450 5.8312325 43.5558 110-119
GGTGA 16280305 5.816266 92.81186 45-49
CCCTG 15425415 5.6284513 57.177288 30-34
GTAAT 9565135 5.4590836 37.385654 110-119
ACAGT 9204240 4.9755716 33.982468 110-119
AGACG 10813875 4.965945 99.88602 40-44
GTAGT 11692030 4.917067 25.209167 70-79
GGCCC 15782055 4.8919396 57.98836 60-69
GTGAC 12192825 4.8567634 82.7164 45-49
GAGAC 10407870 4.779499 129.95906 40-44
CCCCA 10135920 4.7539444 100.40913 60-69
ACCAG 8620930 4.414033 97.39596 45-49
GTGTC 12722015 4.395624 25.430935 120-129
AATAC 5977035 4.3848352 31.937172 110-119
ACTCC 8740460 4.328115 95.764496 30-34
CGTGT 12390590 4.2811136 24.541138 120-129
GCACA 8329550 4.264843 33.821255 110-119
TGACC 9455010 4.1991906 151.5603 45-49
GACCA 7966775 4.0790973 108.85183 45-49
TAGTA 7115680 4.0611124 26.033672 80-89
CCCTT 9437710 4.053715 59.86647 20-24
CTCAG 8930300 3.9661546 30.296663 140-149
CCACT 7986900 3.954966 28.108667 240-247
CACTC 7986705 3.9548695 81.49913 25-29
TGGTG 12702135 3.9362302 26.76439 25-29
CCCAG 9309350 3.91606 83.214005 60-69
AGTAG 8074365 3.9147363 28.281796 70-79
TGAGG 10769140 3.847359 69.65507 35-39
CTCCC 9233805 3.756589 59.062008 30-34
CAGGG 10910985 3.6920884 34.571697 50-59
GACGG 10835875 3.6666722 111.96338 40-44
AGGAG 8872290 3.654225 92.38386 35-39
CTGAG 9103320 3.6261222 97.933624 35-39
CTTGG 10435615 3.6056433 35.123222 20-24
ATGGT 8532580 3.5883646 25.602049 190-199
ACGGT 8943540 3.562477 93.533195 40-44
TGTCT 8730820 3.5510297 20.956724 180-189
TCTTG 8724250 3.5483575 19.674564 170-179
GCCCC 10212980 3.5296445 73.75145 60-69
AGATA 5309420 3.4934428 18.962183 150-159
GAGGA 8259285 3.401747 128.83275 35-39
AGGCT 8479350 3.377576 41.912476 30-34
ATGCA 6206020 3.3548121 80.86893 25-29
CTTCA 6349920 3.3197577 17.482038 200-209
TGTCC 8591735 3.3098361 19.676458 120-129
CCAGG 8771285 3.3092706 39.90192 50-59
GCCCT 8985075 3.2784894 31.600035 20-24
TTCCC 7569610 3.251323 56.486122 50-59
GGGTT 10469805 3.2444599 43.92369 50-59
CCTGG 9854585 3.2249982 32.253452 50-59
TCAGG 8096185 3.22495 20.38807 140-149
CTCTC 7418625 3.1864715 13.809673 100-109
CCGTG 9580435 3.1352804 24.53155 120-129
GATGG 8758285 3.1289654 31.24157 15-19
ACAGA 4999665 3.1158311 14.920298 140-149
GGATG 8648060 3.089587 58.446457 20-24
TGCAC 6920650 3.073622 104.623055 25-29
TCGCA 6899430 3.0641978 25.17758 100-109
GGTTC 8774150 3.031585 49.429825 50-59
CGCAC 7159400 3.0116646 25.669855 100-109
CCAGT 6764975 3.0044832 42.153408 60-69
TACAC 4942625 2.9790215 23.31847 110-119
TCTCA 5676170 2.967519 21.403606 130-139
GACTC 6680010 2.9667482 21.13046 190-199
TGGAG 8212770 2.9340758 29.926311 25-29
TGGCC 8877085 2.9051027 39.49803 60-69
TCTCT 6401405 2.9029229 15.743877 180-189
TCACA 4781950 2.8821795 12.565333 130-139
CCTGA 6454630 2.8666518 67.56102 30-34
TTCTT 5935395 2.8417342 21.796118 170-179
GCACT 6370795 2.8294187 74.18463 25-29
AGGGT 7919130 2.8291705 51.860893 50-59
GTTCC 7312670 2.8170958 56.247753 50-59
CAGAT 5182165 2.801343 15.7145 150-159
CGGAT 6979340 2.7800782 66.06963 20-24
GCCGT 8488070 2.7777944 23.2555 120-129
GGTTG 8920675 2.764404 60.20357 15-19
ACCAC 4833410 2.7592814 15.935973 230-239
CGGTG 9290235 2.7268226 67.74373 40-44
TCAAA 3683530 2.7022884 56.1975 70-79
AGGGA 6501955 2.6779566 9.835303 150-159
CGGCC 8587420 2.6618292 11.404202 120-129
AATAA 2970850 2.6527705 12.679635 110-119
AGTCA 4870120 2.63266 30.296171 70-79
GTTGG 8257970 2.5590403 46.70322 15-19
GGCTG 8641035 2.536273 23.346525 30-34
GATGC 6352120 2.5302374 77.01671 25-29
AGATG 5215875 2.5288398 17.20092 180-189
CTGGC 7625580 2.495537 35.454422 60-69
TGTTC 6105855 2.4833944 13.180151 140-149
GAGAT 5028350 2.4379208 16.884422 180-189
AAAAA 2310320 2.3783133 11.015337 230-239
ACTCT 4544470 2.3758626 13.040573 200-209
GTTCT 5793995 2.3565538 18.85246 160-169
GTCTC 6111545 2.3543804 12.750529 180-189
ACAGC 4589665 2.3499708 12.52154 110-119
TTGTC 5726090 2.3289351 15.3728 170-179
TCATT 4217260 2.3277807 16.35528 150-159
CAGAG 5065710 2.3262742 9.75695 140-149
TTCAT 4193905 2.3148897 17.576658 140-149
CATTT 4169615 2.3014824 17.480587 150-159
CTGGA 5776750 2.301051 15.4847555 180-189
TCCTC 5311750 2.28152 14.8752775 130-139
TGTAG 5368190 2.2575848 8.968438 210-219
TCCAT 4301990 2.2490935 12.086719 70-79
TGAAT 3927975 2.2418025 15.219292 190-199
GGCGG 8985665 2.240505 37.355553 20-24
CACTA 3700210 2.2301924 15.010611 240-247
ATATA 2875035 2.2268152 10.657638 110-119
TAGTC 4742325 2.2236602 25.914307 70-79
TTCAC 4250880 2.2223732 17.93325 200-209
GCGGA 6517760 2.2054968 79.4237 20-24
GAATC 4079035 2.2050197 15.603724 190-199
GTGAA 4502205 2.1828275 17.319168 190-199
AATAT 2782795 2.1553721 12.948824 110-119
ACTAC 3570550 2.1520438 12.533751 230-239
TTGGT 5882480 2.1458468 38.76181 25-29
ATTTG 4304105 2.1307557 15.304397 150-159
CTCTG 5493165 2.1161592 15.306651 180-189
TTGGA 5027255 2.1142051 17.2853 170-179
GAGTC 5279525 2.102991 12.791062 210-219
GATAC 3875635 2.095067 13.855843 150-159
CAGCC 4968200 2.089917 11.284373 120-129
CTACT 3989285 2.0856104 8.381989 230-239
GTCAA 3844915 2.0784607 41.272373 70-79
TCTGC 5370955 2.0690794 10.979644 210-219
GTTCA 4411040 2.068322 15.953672 140-149
TCGGC 6285965 2.0571365 8.811174 190-199
CTCGC 5605535 2.045357 17.155304 100-109
CCTTC 4749435 2.0399926 14.01639 200-209
TTGGG 6552820 2.0306358 73.781815 15-19
ATATG 3556865 2.0299997 9.8407755 180-189
GATAT 3553525 2.0280936 9.56493 180-189
TCTCG 5256220 2.0248797 16.743683 100-109
AATAG 3066045 2.0173678 8.9049425 240-247
ACACA 2855800 1.9843656 13.862283 110-119
AATCG 3664905 1.9811518 14.142602 190-199
ATAGA 3009860 1.9803997 10.288512 240-247
CACGG 5241790 1.9776464 7.7509856 200-209
TACAG 3647500 1.9717432 11.991253 160-169
GTTGT 5287790 1.9289123 7.5849743 210-219
TCCCA 3894585 1.928527 11.41243 240-247
TTGCA 4103730 1.9242254 13.586655 150-159
CCTTG 4955605 1.9090723 39.654655 20-24
TACCA 3163085 1.9064562 12.176441 230-239
TACTA 2982890 1.8981338 10.962058 230-239
GGTCA 4760860 1.8963917 10.085478 130-139
AGTAT 3303505 1.8854004 12.176203 220-229
GTCCT 4878950 1.8795419 12.685657 130-139
GAACT 3467160 1.8742561 11.659854 160-169
ATTGT 3766150 1.8644397 8.881993 170-179
GTCAC 4197255 1.8640988 9.509528 130-139
GCTGT 5385860 1.8608862 10.204483 140-149
TGGAA 3824095 1.8540558 9.315357 170-179
TTTGC 4553305 1.8519361 11.738722 150-159
GCTGA 4646455 1.8508208 36.39289 30-34
CAGGC 4850085 1.8298624 14.169383 140-149
AGTCT 3896175 1.8269036 13.369662 210-219
CATAG 3341545 1.8063519 8.540131 210-219
CTGTT 4431855 1.8025396 12.786759 140-149
TGGGG 6809315 1.7925557 44.408936 15-19
TCAGC 4029195 1.7894595 11.78313 140-149
GTCTG 5103055 1.7631731 9.109569 210-219
TTGTA 3556955 1.7608774 10.095088 210-219
ATGTA 3071135 1.7527804 10.216191 70-79
ATAGT 3069450 1.7518188 7.28549 210-219
AGAAC 2782415 1.7340231 11.381974 160-169
TCTGG 4945295 1.708665 12.51149 180-189
CAGCT 3844330 1.7073566 9.552516 150-159
CACCA 2990005 1.7069246 11.363338 230-239
GCAGA 3708670 1.7030945 13.975232 150-159
ACGGC 4490145 1.6940625 12.741532 120-129
TTCAG 3591210 1.6839064 10.208538 150-159
GGGGC 6684560 1.6667426 41.02016 15-19
ATCTC 3180025 1.6625267 6.834586 100-109
TGACT 3534565 1.6573458 12.338851 190-199
CCCAC 3528905 1.6551253 10.971361 240-247
TGCAG 4142175 1.6499511 12.814285 150-159
GACGT 4140890 1.6494393 10.248535 170-179
GAGAA 2925745 1.6353416 11.206056 160-169
GGAGG 5372880 1.6306258 25.597527 30-34
CTCCA 3291840 1.6300588 8.749657 240-247
AACTG 3005965 1.6249461 10.873526 160-169
GTGGA 4542120 1.6227075 36.79102 25-29
TGGTT 4426015 1.6145486 17.412537 10-14
GGACG 4744190 1.6053517 8.114481 170-179
CACTG 3605185 1.6011467 6.915991 230-239
CCAGA 3108385 1.5915354 22.167294 60-69
GGGCG 6382185 1.5913477 48.443024 20-24
AACCA 2289125 1.5906088 12.305553 70-79
CAAAG 2548765 1.5884105 28.89038 70-79
ATAAA 1767130 1.577929 6.2741985 110-119
ATAAT 2036005 1.5769572 5.525594 110-119
CGACT 3521735 1.5640846 9.602186 190-199
CAAAA 1847080 1.56218 20.142189 70-79
TCGAC 3484540 1.5475655 10.325655 190-199
AGCTC 3468520 1.5404506 9.61089 140-149
CTCTT 3394975 1.5395606 9.300323 200-209
ACGGA 3351270 1.5389694 10.145091 200-209
ACTGA 2835955 1.5330431 7.5909057 180-189
GTCCC 4187475 1.527933 16.10965 50-59
CCTCG 4137025 1.5095247 9.145123 130-139
CTGGT 4327160 1.4950913 7.257257 160-169
CTTGA 3183500 1.4927326 9.768437 200-209
GCTCT 3836880 1.4781001 9.603138 130-139
ATCGG 3707430 1.4767791 9.775505 190-199
TCCAC 2979410 1.4753492 10.662863 240-247
TCAGA 2728400 1.4749018 8.981549 130-139
ATGGG 4122335 1.4727362 50.640648 15-19
GAGGG 4842655 1.4697067 9.679688 200-209
ACACG 2869975 1.4694662 9.620124 110-119
AAATA 1643380 1.4674288 5.1650534 6
CCACC 3109700 1.4585099 8.450049 230-239
AATTG 2546820 1.4535395 9.831262 170-179
TGGAC 3623365 1.4432936 11.10845 170-179
GGCCG 5183815 1.4411398 12.137363 120-129
CTCGA 3239970 1.4389462 9.232221 190-199
AGCCG 3807585 1.4365431 10.802713 120-129
CCATC 2883675 1.427943 10.97489 240-247
TAGTT 2873500 1.4225316 8.868656 220-229
GGGAG 4609255 1.3988719 7.0870066 150-159
CGTAG 3494850 1.3921024 8.406631 210-219
GAATT 2398155 1.3686925 10.80547 170-179
TATAC 2142660 1.3634614 6.7644434 110-119
TATGG 3228045 1.3575498 6.9111814 180-189
CAATA 1842210 1.3514707 7.5696564 100-109
ACTAT 2113560 1.3449439 7.156016 230-239
GGCTC 4094845 1.3400735 8.71091 130-139
GTGCT 3819995 1.3198589 7.756282 220-229
ACGTG 3309800 1.3183914 9.680834 170-179
ACTCG 2966800 1.317625 9.342447 190-199
CATGT 2804810 1.315166 9.715174 70-79
CAGAC 2561665 1.3116074 11.29323 60-69
ATCCA 2171590 1.3088619 16.72385 240-247
AAGTA 1978160 1.3015712 17.581835 70-79
ACCAT 2152215 1.297184 9.812171 45-49
ACTGT 2755040 1.291829 5.5821447 230-239
ACTTC 2450260 1.2810034 5.78286 220-229
TGATA 2236000 1.2761462 7.0558834 180-189
GAGGC 3759795 1.2722493 22.478872 30-34
AAAGT 1932720 1.2716731 21.57417 70-79
AGAGT 2620605 1.2705616 5.0099354 200-209
GCTCA 2860405 1.2703725 9.694862 140-149
GTGTT 3482285 1.2702891 7.432353 160-169
TTCCA 2424680 1.2676302 7.366703 230-239
CCATG 2800430 1.2437363 9.82523 70-79
TGTGC 3591960 1.2410698 6.3182645 220-229
GGAGT 3451080 1.2329252 6.9900093 210-219
GGAAT 2523095 1.2232853 8.619279 170-179
CCTCA 2457650 1.2169832 5.9503546 130-139
TTGAG 2892515 1.2164432 8.865678 200-209
GAACC 2375485 1.2162806 9.007148 70-79
GGTGG 4598535 1.2105669 36.662804 25-29
GGGCC 4319175 1.2007633 28.456633 15-19
GTCCA 2700315 1.1992729 11.22979 70-79
AGTTC 2553010 1.1970979 5.8647494 160-169
CTGAT 2546955 1.1942588 6.282472 180-189
TATCA 1873785 1.1923653 5.6429377 70-79
GCGTA 2987065 1.1898364 9.433941 210-219
ACTGG 2969680 1.1829115 7.272099 160-169
AGAGA 2103965 1.1760087 12.352238 35-39
CTGCG 3531300 1.1556485 6.666216 210-219
TGGGC 3934615 1.1548681 30.380539 15-19
TCACG 2598045 1.1538523 8.943642 200-209
GTCTA 2443960 1.1459647 7.898468 180-189
CTGAA 2096190 1.1331457 13.27428 35-39
TATGT 2281095 1.1292604 7.7483354 220-229
GCATA 2072675 1.120434 7.660124 210-219
TGCCC 3068150 1.1195117 9.16448 60-69
TAGAT 1953680 1.1150185 8.207167 240-247
ACAAT 1519375 1.1146345 8.438169 100-109
TCTAC 2128565 1.1128204 7.582938 180-189
CCCAA 1935920 1.1051718 9.777843 240-247
AGATC 2040635 1.103114 5.572731 130-139
GAAGA 1957640 1.0942205 17.659462 35-39
AGGTT 2564545 1.0785159 18.533922 10-14
AGAGC 2345360 1.0770357 6.729893 140-149
GCGGT 3659465 1.0741075 7.124961 130-139
CGGCT 3280990 1.0737323 7.706238 130-139
CTCGG 3263635 1.0680528 7.5780625 130-139
CCAAT 1764450 1.0634702 11.04813 240-247
TTGGC 3060565 1.0574658 5.039331 170-179
AAAAG 1389260 1.0538238 13.309664 70-79
TATAA 1360205 1.0535265 7.498002 240-247
ATAAC 1410790 1.034975 7.0872903 80-89
TGGTC 2961785 1.0233358 8.042172 50-59
GTGGT 3286540 1.0184569 7.0615726 50-59
AAGAG 1803185 1.0078881 12.267625 35-39
TGTAA 1765145 1.0074164 6.9797363 230-239
TACTG 2145360 1.0059521 5.293889 180-189
CGGTC 3066340 1.0034863 7.2325406 130-139
ATATC 1568190 0.99790275 5.7029653 70-79
TCTGT 2439190 0.99207586 5.431029 210-219
CGGAG 2928020 0.99079126 6.5790734 210-219
CATCA 1639355 0.98807293 5.718517 230-239
GCCAC 2344220 0.98611677 5.0629244 80-89
ATACG 1812255 0.9796577 5.0391126 120-129
GCTTC 2538980 0.97810376 5.717582 150-159
AGCTT 2084645 0.97748315 6.0838776 150-159
GAGTT 2291295 0.96360093 5.483773 160-169
ACCCA 1669975 0.95335 12.268855 240-247
ACTAA 1298310 0.95245814 11.849452 240-247
CTACC 1867835 0.9249175 6.806681 230-239
CGATG 2312495 0.9211353 21.454199 15-19
TCCAG 2066640 0.91784304 5.6431355 240-247
ACCGT 2051105 0.91094345 12.929762 45-49
TAGAC 1682820 0.90968853 5.096195 110-119
CTTCC 2111590 0.9069769 5.1986055 230-239
TGAAG 1836505 0.89040226 9.411763 35-39
GAGCT 2207065 0.8791393 5.5523777 140-149
CCAGC 2074245 0.8725494 5.8643074 80-89
ATCAA 1170830 0.8589371 12.38986 70-79
TGCAT 1821000 0.85386086 6.2541113 210-219
TGCGT 2462120 0.8506952 7.2284937 210-219
AGCTG 2127930 0.8476176 6.818755 30-34
AATCC 1401380 0.84464043 10.660617 240-247
TGAAC 1554045 0.84007615 5.4549456 190-199
AGCCA 1600415 0.8194342 7.762075 80-89
GTATG 1937075 0.8146342 5.261846 220-229
AGACC 1554955 0.796158 5.4400973 240-247
CCTTT 1738090 0.7881928 6.177751 50-59
CTAAT 1231410 0.7835961 9.515228 240-247
AAAGG 1387705 0.77565604 7.3896956 10-14
GGAAG 1861390 0.7666497 9.57385 30-34
TAACC 1259515 0.7591355 6.5536814 80-89
ACGTC 1686640 0.7490761 7.3878007 70-79
CAATC 1242225 0.7487145 10.548225 240-247
TAGGT 1745610 0.7341139 6.266521 10-14
TCGAA 1351605 0.7306424 12.862204 70-79
TGAGA 1501000 0.7277376 5.933808 240-247
ATACC 1199310 0.7228487 6.4899125 240-247
AGGAT 1482925 0.7189742 8.128336 10-14
GCGGC 2534285 0.7045503 5.0945992 130-139
TGGAT 1661660 0.6988088 6.588549 10-14
AGCGT 1740855 0.6934342 5.0839796 160-169
GTCGA 1702155 0.6780188 8.801918 70-79
GACCG 1787485 0.67439055 13.13533 45-49
GGTCC 2053565 0.67204696 8.890495 50-59
AATGG 1359505 0.6591358 5.334647 10-14
CGAAC 1282970 0.6568981 9.018148 70-79
ACGAT 1189810 0.6431802 8.190617 10-14
AAGGT 1318770 0.6393861 6.7817154 10-14
CATCC 1237550 0.612812 7.302349 240-247
CTTTG 1500170 0.6101544 5.34862 50-59
AAAGC 976545 0.60859054 8.418343 70-79
CGTCC 1558095 0.5685203 5.8306417 70-79
ATAGG 1169895 0.5672062 5.3447037 10-14
GAAGC 1227655 0.56376344 8.755239 30-34
TCAAG 1025600 0.55441254 5.909841 70-79
TTGCC 1414140 0.5447761 6.111433 60-69
AAGCT 995685 0.53824127 14.17302 30-34
GAAAA 686250 0.52055526 5.6127396 6
GACGA 1117910 0.51336634 6.4620275 40-44
GGGAT 1342405 0.47958466 5.950137 10-14
CGGTT 1358500 0.46937975 9.612784 10-14
TAAAG 703345 0.4627804 5.697948 9
AAGCA 738455 0.460211 5.5100126 70-79
TCGAT 902555 0.42320505 6.56132 10-14