FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_4_SL254631.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_4_SL254631.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33401953
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 39020900 8.293398 56.61092 110-119
CACAG 49769860 7.1275697 40.545597 110-119
TCCCT 52389190 6.2927566 87.63265 30-34
CAGTA 41806125 6.267882 39.070633 60-69
ATACA 30570110 6.2062097 29.310627 110-119
GGAGA 53866205 6.1548624 91.98896 35-39
AGTAA 32006610 5.804069 42.26894 110-119
GGTGA 58329230 5.768754 93.718 45-49
GTAAT 34468335 5.410121 36.36473 110-119
CCCTG 52048475 5.334146 52.632046 30-34
AGACG 40400575 5.1680427 102.31955 40-44
GGCCC 58977545 5.157048 58.208717 60-69
ACAGT 32963450 4.942123 33.661415 110-119
CCCCA 37019600 4.907173 104.176476 60-69
GTAGT 42049235 4.87412 24.858198 80-89
GTGAC 43316735 4.7960978 84.157684 45-49
GAGAC 37297365 4.77108 133.77527 40-44
CCCTT 36964670 4.440032 70.07108 20-24
GTGTC 46160365 4.423799 24.608147 120-129
TAGTA 27371075 4.296141 30.11781 80-89
TGACC 34581045 4.2865415 153.06487 45-49
ACCAG 29914975 4.2841406 94.265465 45-49
AATAC 21004160 4.2641726 29.7183 110-119
CGTGT 44350495 4.2503486 23.622522 120-129
GCACA 29008510 4.1543255 34.77459 110-119
GACCA 28350620 4.0601087 106.61606 45-49
TGGTG 46869720 4.0121937 31.021727 25-29
CCCAG 33303375 3.943223 85.36573 60-69
ACTCC 28346315 3.9337075 88.210884 30-34
AGTAG 29247825 3.9168587 26.665335 70-79
CTCAG 31160865 3.862588 29.659134 140-149
CTTGG 39686920 3.8034136 40.497974 20-24
GACGG 39951425 3.7741768 112.99514 40-44
TGAGG 38028920 3.7610557 69.89316 35-39
CCACT 26873355 3.7293005 26.75545 240-247
GCCCC 37683520 3.6889482 76.54438 60-69
AGGAG 31618805 3.612829 91.55605 35-39
CTGAG 32619510 3.6116843 98.21082 35-39
CACTC 25858635 3.5884843 73.27952 25-29
ACGGT 32372680 3.5843546 93.18994 40-44
TGTCT 31746750 3.5658886 21.684296 180-189
CAGGG 37700335 3.5615182 33.591812 50-59
CTCCC 31015650 3.5585604 55.2821 30-34
AGGCT 31796170 3.5205226 48.6077 30-34
ATGGT 30340885 3.5169513 25.713692 190-199
GCCCT 34256240 3.5107234 37.340973 20-24
AGATA 19282360 3.4966571 18.943209 150-159
TCTTG 31066735 3.4895074 20.060202 170-179
TGTCC 31831285 3.4152057 18.67663 120-129
GAGGA 29797380 3.4047093 128.6698 35-39
GATGG 33662145 3.3291821 36.196655 15-19
CCAGG 30760430 3.253261 38.915836 50-59
CCGTG 35500580 3.2497938 24.035175 120-129
TCAGG 29172535 3.2300296 20.172325 140-149
CTCTC 26848790 3.2249575 13.514002 130-139
CTTCA 22185730 3.2231832 17.423115 200-209
ACAGA 18269780 3.1646118 14.297628 140-149
TTCCC 25797990 3.0987399 53.767067 50-59
TGGAG 31213570 3.0870183 34.09381 25-29
TCGCA 24811515 3.0755458 23.838993 100-109
CCTGG 33596530 3.075493 30.907452 50-59
CGCAC 25646660 3.0366442 24.18666 100-109
ATGCA 20234980 3.033777 73.41762 25-29
TCTCT 24037855 3.02274 16.935343 180-189
GGGTT 35248665 3.0173953 41.25551 50-59
AATAA 12050850 2.9590964 14.211451 110-119
TCTCA 20205835 2.9355404 20.133844 130-139
GACTC 23653625 2.9320178 19.48369 190-199
GGATG 29504455 2.917987 51.999256 20-24
TGGCC 31676255 2.8997073 39.32906 60-69
GGTTC 29888235 2.8643522 46.657223 50-59
TACAC 17030345 2.858518 20.62348 110-119
TTCTT 21472125 2.8267403 22.320642 170-179
TGCAC 22630695 2.8052192 94.34768 25-29
CAGAT 18709890 2.8051243 15.957633 150-159
GCCGT 30360495 2.7792602 23.008255 120-129
CGGTG 33889285 2.7710617 67.6341 40-44
CCAGT 22337295 2.7688503 39.253086 60-69
TCACA 16463205 2.7633243 12.092102 140-149
TCAAA 13526360 2.7460623 56.27667 70-79
GTTCC 25382345 2.7232935 53.58251 50-59
GGCTG 32948780 2.694158 27.085806 30-34
ACCAC 16797325 2.6930952 15.600165 230-239
CGGCC 30785465 2.6919084 11.349916 200-209
AGGGT 27103805 2.680563 49.2669 50-59
CCTGA 21612835 2.6790488 61.9997 30-34
AGATG 19730580 2.6423125 17.18017 180-189
CGGAT 23539115 2.6062884 58.920242 20-24
GCACT 20864870 2.5863338 66.29679 25-29
AGGGA 22567510 2.5786097 9.11793 150-159
TGTTC 22607000 2.5392852 13.020483 150-159
GGTTG 29593835 2.5333242 54.55032 15-19
GAGAT 18845340 2.5237615 16.985994 180-189
AGTCA 16429530 2.463236 28.646652 70-79
GTCTC 22698055 2.4352937 13.650164 180-189
TTGTC 21619260 2.428339 15.1046915 170-179
CTGGC 26521550 2.4278357 34.535618 60-69
TCATT 15869470 2.4136786 17.584902 150-159
CATTT 15858410 2.4119966 18.768312 150-159
ACAGC 16764250 2.4008179 12.796555 120-129
TTCAT 15410595 2.343886 16.980764 140-149
TCCTC 19385175 2.3284612 15.922362 130-139
CTGGA 21002930 2.325478 15.7600975 180-189
ACTCT 16005785 2.3253493 12.607098 200-209
TCCAT 15994340 2.3236866 14.587665 70-79
GTTCT 20684210 2.3233116 17.349539 160-169
CAGAG 18125930 2.3186693 8.958925 140-149
GTTGG 26981200 2.309675 42.371624 15-19
AATAT 10861710 2.3085191 13.364331 110-119
GATGC 20802165 2.303249 70.20669 25-29
ATATA 10776405 2.2903883 11.040188 110-119
TTCAC 15654475 2.2743104 18.196695 200-209
TTGGT 22469850 2.2544057 44.68514 25-29
GTGAA 16794800 2.2491536 17.857225 190-199
TGTAG 19314330 2.2388127 8.681357 70-79
CACTA 13133320 2.204408 15.487188 240-247
ATTTG 16189090 2.1993933 16.27251 150-159
GAATC 14614370 2.191094 15.918558 190-199
CCTTG 20350745 2.1834488 45.92954 20-24
TGAAT 13888705 2.1799595 15.3710575 190-199
CTCTG 20189175 2.1661139 15.701755 180-189
GAGTC 19371655 2.1448605 13.620521 210-219
GGCGG 30517645 2.129082 34.260494 20-24
TTGGA 18299665 2.1211982 17.06381 170-179
TCTGC 19729095 2.1167517 11.179112 210-219
TAGTC 16217015 2.1044838 23.456175 70-79
TCGGC 22907495 2.096998 8.818674 190-199
GTTCA 16095280 2.0886865 15.713545 140-149
CAGCC 17588970 2.0825887 11.576162 120-129
GATAC 13873545 2.080024 13.352588 150-159
AATCG 13774070 2.06511 14.186641 190-199
ACTAC 12254400 2.0568826 11.767644 230-239
CCTTC 17101715 2.054182 14.412673 200-209
AATAG 11324350 2.053554 8.351392 240-247
CTCGC 20022250 2.051964 16.310947 100-109
GCTGA 18402095 2.0375092 42.661045 30-34
GTCAA 13586585 2.0370007 39.263206 70-79
GCGGA 21404765 2.0220897 71.76208 20-24
ATAGA 11090150 2.011084 9.857544 240-247
TACAG 13357330 2.0026293 12.390991 160-169
ATATG 12753930 2.001846 8.787963 180-189
TCTCG 18637115 1.9995921 15.906704 100-109
CACGG 18897000 1.9985701 8.042592 200-209
ACACA 10268460 1.9912645 13.185898 110-119
TTTGC 17724345 1.9908507 12.234958 150-159
TTGCA 15268985 1.9814576 14.052023 150-159
TGGAA 14689455 1.9672066 10.136626 25-29
GTCCT 18121920 1.944316 13.791901 130-139
ATTGT 14169695 1.9250454 8.819517 170-179
GCTGT 19931565 1.91015 10.199697 140-149
CTACT 13069460 1.8987547 7.483289 230-239
GTTGT 18921360 1.8983852 7.0749764 210-219
GATAT 12056640 1.8924001 8.635078 180-189
CAGGC 17840725 1.8868572 14.575377 140-149
AGTAT 11937555 1.8737087 12.152115 220-229
TCCCA 13485295 1.8713969 11.70995 240-247
GGTCA 16895240 1.870668 9.587943 140-149
AGTCT 14411805 1.8702216 13.884285 210-219
TTGGG 21553020 1.8450055 66.00487 15-19
CTGTT 16374240 1.8392031 12.766286 140-149
TACCA 10854105 1.8218452 11.606497 230-239
GTGGA 18265350 1.806441 42.24303 25-29
GTCAC 14478190 1.794664 9.323183 140-149
ATAAT 8441890 1.794217 6.4853463 110-119
TACTA 10164990 1.7862005 10.316052 240-247
CATAG 11875265 1.7804271 7.77714 210-219
GCAGA 13911100 1.7795081 14.175153 150-159
TCTGG 18526905 1.7755343 12.944044 180-189
ATGTA 11301545 1.7738811 12.157399 70-79
GTCTG 18251905 1.7491794 9.303374 210-219
ATAGT 11135295 1.7477864 7.1203623 220-229
ATAAA 7059790 1.7335374 6.8207464 110-119
GAACT 11496900 1.7236998 10.46382 160-169
CCAGA 11934775 1.7091861 26.324987 60-69
GGAGG 20163280 1.7014321 29.673502 30-34
TGCAG 15360095 1.7006942 13.235087 150-159
AAAAA 5994320 1.7005427 7.376637 7
GTCCC 16559825 1.6971203 19.39883 50-59
TTGTA 12485030 1.6961727 9.234093 220-229
CAAAA 7215395 1.6923717 22.122416 70-79
AGAAC 9731545 1.6856558 10.794019 160-169
GACGT 15193530 1.6822518 9.610626 170-179
CACCA 10467765 1.6782844 11.640911 230-239
ACGGC 15717490 1.6623012 12.118509 120-129
ATGGG 16665360 1.6482021 58.449783 15-19
ACGGA 12866720 1.645911 10.358827 200-209
TGGGG 22379575 1.6345532 39.50967 15-19
TCAGC 13184620 1.6343179 10.382678 140-149
AACCA 8411865 1.6312327 12.328238 70-79
AAATA 6592695 1.6188418 5.824274 6
TTCAG 12421000 1.6118746 9.776502 150-159
GGACG 17055520 1.6112202 7.7671757 170-179
CCCAC 12131605 1.608118 10.804717 240-247
GAGAA 10317505 1.5963393 10.757915 160-169
CAGCT 12872265 1.5955994 8.69802 150-159
TGACT 12250710 1.589776 11.2617445 190-199
ATCTC 10933590 1.5884517 6.9100447 100-109
CGACT 12785655 1.5848635 9.297733 200-209
TCGAC 12668745 1.5703719 9.530423 190-199
CCTCG 15268300 1.5647595 9.970959 130-139
AACTG 10379070 1.5561066 10.192283 160-169
CACTG 12454410 1.5438035 6.237896 230-239
CTCTT 12237695 1.5388798 8.875254 200-209
CAAAG 8836195 1.530567 27.93572 70-79
GGGGC 21892150 1.5273193 36.872784 15-19
AGCCG 14301940 1.5125909 11.207548 120-129
AATTG 9603190 1.5073086 9.888492 170-179
CTCCA 10774965 1.4952759 8.014059 240-247
ACTGA 9958740 1.4930875 7.092904 180-189
TGGTT 14814930 1.4863858 14.765088 10-14
GCTCT 13797505 1.480346 9.236502 130-139
CAATA 7249435 1.4717485 7.9833574 100-109
GGGCG 21088100 1.4712243 44.57003 20-24
CAGAC 10206800 1.4617217 13.997075 60-69
TCAGA 9739250 1.46018 8.191375 130-139
AGCTC 11734100 1.4545166 8.908434 140-149
CTCGA 11730795 1.4541068 8.643377 190-199
GAATT 9157235 1.437312 10.984478 170-179
CCACC 10823530 1.4347247 8.35816 230-239
GGCCG 18344805 1.4328179 11.789306 120-129
ATCGG 12894410 1.4276897 9.713065 190-199
TCCAC 10240245 1.421071 9.332948 240-247
CTGGT 14724940 1.4111712 6.6116934 160-169
TGGAC 12696380 1.4057634 10.708481 170-179
GAGGC 14828205 1.4008077 26.193722 30-34
CCATC 10039380 1.3931965 10.738469 240-247
CGTAG 12548990 1.3894442 7.990986 210-219
GAGGG 16359940 1.3804959 8.576399 200-209
ACCAT 8215000 1.3788753 11.392684 45-49
GGGCC 17580120 1.3730927 33.149006 15-19
GGTGG 18701785 1.3659358 42.54118 25-29
CATGT 10493195 1.3617032 11.809318 70-79
GTCCA 10956730 1.3581566 13.567816 70-79
ACTAT 7727530 1.357888 7.3723803 230-239
GGGAG 15756960 1.3296149 6.433822 150-159
GGCTC 14517885 1.3289961 8.366529 130-139
TGGGC 16251880 1.328885 35.158028 15-19
CTTGA 10183430 1.321505 9.012816 200-209
AAGTA 7254175 1.3154697 17.42453 70-79
CCATG 10562515 1.3092911 11.9440155 70-79
TTCCA 8936915 1.2983713 7.8716626 230-239
TATGG 11199450 1.2981797 6.5071125 190-199
ACACG 9040800 1.2947382 8.649361 120-129
TAGTT 9511800 1.29224 7.8718095 220-229
GGAGT 13040430 1.2896969 7.5873017 210-219
AGAGA 8320840 1.2874124 14.174803 35-39
TATCA 7320460 1.2863573 6.3358135 70-79
ACTCG 10376395 1.2862203 8.921589 190-199
TATAC 7319225 1.2861403 6.4737635 110-119
AGAGT 9453700 1.2660363 5.070005 210-219
CCTCA 9099200 1.2627246 5.8450212 130-139
ACGTG 11374565 1.25941 9.030299 170-179
GTGTT 12549315 1.2590761 7.576307 160-169
ACTTC 8653105 1.2571387 5.640244 220-229
AAAGT 6907925 1.2526809 20.804995 70-79
GAACC 8738535 1.2514507 9.050985 70-79
ACAAT 6119315 1.2423165 8.843591 100-109
TCACG 9855750 1.221683 9.174952 200-209
TGCCC 11900440 1.219607 11.42666 60-69
GGAAT 9055725 1.2127396 8.802924 170-179
ATCAT 6883865 1.2096385 5.1574845 90-99
GCGTA 10873955 1.2039818 9.182988 210-219
CTGCG 13135030 1.2024068 6.5360775 210-219
GTGCT 12542290 1.2019958 6.6997004 220-229
TTGAG 10349970 1.1997125 8.156442 200-209
AGTTC 9231330 1.1979507 5.8163133 160-169
GCTCA 9557435 1.184705 8.458485 140-149
TGATA 7515385 1.1796086 6.1686444 180-189
CAGTG 10428580 1.154669 5.06847 160-169
AAAAG 5453555 1.1425593 14.478494 70-79
TGTGC 11838385 1.1345369 5.8706627 220-229
GCATA 7479870 1.1214371 7.3884125 210-219
TATAA 5259595 1.1178602 8.199503 240-247
ACTGG 10076770 1.115716 6.7585387 160-169
TATGT 8150330 1.1072755 7.3455644 220-229
CTCGG 12052590 1.1033181 7.861542 130-139
CTGAT 8464060 1.098382 5.5922627 180-189
CGGCT 11988050 1.09741 7.7026324 130-139
GCGGT 13373040 1.0934877 6.821598 130-139
TGGTC 11360290 1.0887184 9.763213 50-59
GTCTA 8361125 1.0850241 7.3468165 180-189
CTGAA 7234125 1.0845932 15.512714 35-39
TTGGC 11245680 1.0777346 5.0370903 170-179
ATAAC 5302735 1.0765381 7.128039 80-89
CCCAA 6700975 1.0743595 9.073237 240-247
GTGGT 12547925 1.0741414 8.545319 50-59
CCAAT 6327470 1.0620563 10.283102 240-247
ATCCA 6303865 1.0580943 14.294648 240-247
GAAGA 6797630 1.0517391 20.1821 35-39
CATCA 6255020 1.0498956 6.550743 240-247
CGATG 9472530 1.0488137 24.715519 15-19
ACCGT 8377795 1.0384811 15.586507 45-49
TCTAC 7115510 1.0337542 6.823333 180-189
TAGAT 6563565 1.0302117 7.8151274 240-247
CGGAG 10895600 1.029298 7.160679 210-219
AGGTT 8811825 1.0214192 16.703781 10-14
CGGTC 11026595 1.0093964 6.777434 130-139
CCATT 6918735 1.0051664 5.395918 50-59
AAAAT 4058270 0.99651164 5.435889 5
TAGAC 6552700 0.98242897 5.3443627 110-119
GAGTT 8425330 0.9766187 5.3320475 160-169
AAGAG 6252520 0.96739894 14.074468 35-39
ATATC 5490640 0.9648198 6.2617607 70-79
TCTGT 8567045 0.96227586 5.044159 210-219
AGAGC 7494355 0.95867807 5.8769584 140-149
TGAAG 7083950 0.94868016 10.937538 35-39
ATCAA 4612710 0.9364521 14.162406 70-79
TGTAA 5922375 0.92957115 6.1628532 230-239
GCTTC 8425480 0.903977 5.042283 150-159
CCTTT 7160105 0.9003771 7.2273955 50-59
ACTAA 4396890 0.8926373 11.538114 240-247
CTACC 6426745 0.89185965 6.7875495 230-239
CACAA 4592015 0.8904856 5.452834 110-119
AGCTT 6779250 0.87974405 5.3414516 150-159
CTTCC 7246305 0.870394 5.460908 230-239
TGCGT 8978250 0.8604345 6.7617188 210-219
AGCTG 7673835 0.8496593 8.099648 30-34
TGCAT 6434675 0.8350285 6.0215564 210-219
GAGCT 7311710 0.8095642 5.051509 140-149
CCAGC 6777935 0.80252856 5.5695386 80-89
TAACC 4775200 0.80151016 6.4950624 80-89
AGCCA 5519675 0.79047585 7.1953893 80-89
ACCCA 4929945 0.79041225 10.621664 240-247
AAAGA 3744500 0.7844999 5.042815 8
AAATG 4304215 0.7805249 5.2342525 9
GGTCC 8507310 0.7787761 10.902944 50-59
ACGTC 6201195 0.7686777 8.860152 70-79
GGAAG 6696665 0.76517457 11.51236 30-34
TGAAC 5100185 0.76465726 5.5010614 190-199
CGGCG 9785880 0.76432467 5.0726166 130-139
TCGAA 5069935 0.76012194 13.038124 70-79
AGGAT 5674920 0.75998336 9.4674015 10-14
TGAGA 5591620 0.74882776 5.419955 240-247
GCGGC 9550285 0.7459236 5.231903 130-139
AATCC 4437170 0.7447723 9.234937 240-247
GAAGT 5559390 0.7445116 5.42995 70-79
AAAGG 4799800 0.742632 7.5005608 10-14
GTATG 6385960 0.740226 5.0881796 220-229
ACGAT 4934230 0.7397761 9.625031 10-14
GACCG 6885950 0.7282666 15.700163 45-49
CAATC 4335180 0.72765344 9.413332 240-247
TAGGT 6263845 0.72607106 5.5760946 10-14
CTAAT 4062430 0.71385354 9.258005 240-247
CTTTG 6330490 0.71105933 6.283906 50-59
GTCGA 6321845 0.69996476 8.665496 70-79
ATACC 4163410 0.6988221 5.7220674 240-247
CGAAC 4810695 0.68894243 8.981597 70-79
AAAGC 3975275 0.68857974 9.522585 70-79
CGTGG 8327905 0.6809567 5.9078703 50-59
CGTCC 6555875 0.67187357 7.1806984 70-79
TTTCG 5911705 0.66402006 5.1558414 100-109
AATGG 4954875 0.6635551 5.3718224 10-14
TGGAT 5716210 0.6625922 7.487825 10-14
TTGCC 6024520 0.64637583 7.5881786 60-69
GAAGC 4987920 0.63805485 10.578606 30-34
GATGA 4547120 0.6089487 5.2415266 45-49
ATAGG 4455960 0.5967406 5.3132205 10-14
AAGGT 4420180 0.5919489 6.171446 10-14
AAGCT 3947430 0.59182775 16.709 30-34
GACGA 4434395 0.56724787 7.507805 40-44
CATCC 3944670 0.5474143 6.3977213 240-247
TCAAG 3508200 0.52597517 5.718364 70-79
AAGCA 2920450 0.5058676 6.223841 70-79
GAAAA 2382270 0.4991029 5.025225 6
GGGAT 5023615 0.49683493 6.5568156 10-14
CGGTT 5032525 0.48229426 9.231015 10-14
TAAAG 2610840 0.47344893 5.294036 9
TCGAT 3446855 0.44729877 7.322545 10-14
GCGAT 3039510 0.3365394 5.561264 10-14