FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s1_1_illumina12index_7_SL254634.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s1_1_illumina12index_7_SL254634.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11972662
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13827170 8.348609 58.450726 110-119
CACAG 18124895 7.191881 41.190796 110-119
CAGTA 15486210 6.4993267 42.27676 60-69
TCCCT 19305910 6.4490147 92.571655 30-34
ATACA 11075520 6.3224983 32.2197 110-119
GGAGA 19458590 6.192425 93.52686 35-39
AGTAA 11507380 5.8829107 43.43311 110-119
GGTGA 21064895 5.820661 92.81623 45-49
CCCTG 19469765 5.506794 55.241463 30-34
GTAAT 12342735 5.4788685 37.45553 110-119
ACAGT 11973720 5.025188 34.014935 110-119
AGACG 14096150 5.0090885 100.19013 40-44
GGCCC 20708865 4.959402 57.72178 60-69
GTAGT 15099415 4.927631 24.789171 70-79
GTGAC 15825690 4.8829756 83.39347 45-49
CCCCA 13155890 4.7852373 101.43119 60-69
GAGAC 13431575 4.772931 130.52718 40-44
ACCAG 11235095 4.458038 97.412674 45-49
GTGTC 16609880 4.4499183 25.345705 120-129
AATAC 7732960 4.4143863 32.157093 110-119
CGTGT 16017485 4.2912107 24.340319 120-129
TGACC 12317170 4.24367 152.77855 45-49
GCACA 10655000 4.227859 33.80195 110-119
CCCTT 12630170 4.219027 64.740944 20-24
ACTCC 10839085 4.1699615 91.13069 30-34
TAGTA 9351235 4.1509585 27.79802 80-89
GACCA 10231185 4.059691 109.01037 45-49
TGGTG 16665080 3.9983804 28.550621 25-29
CTCAG 11447795 3.9441419 30.17246 140-149
CCCAG 12087280 3.937341 83.909996 60-69
AGTAG 10475615 3.9372585 27.454594 70-79
CCACT 10127375 3.8961563 27.774662 240-247
TGAGG 13928470 3.8487215 69.81979 35-39
CACTC 9909765 3.8124382 78.2619 25-29
CTTGG 13884320 3.7197192 38.070427 20-24
GACGG 14209420 3.7122157 112.123764 40-44
CTCCC 11601130 3.6639304 56.90851 30-34
CAGGG 13967720 3.6490715 33.99718 50-59
AGGAG 11464545 3.6484318 93.04569 35-39
CTGAG 11802535 3.6416411 97.85595 35-39
ATGGT 10960680 3.5769718 25.417345 190-199
GCCCC 13373370 3.5762079 74.29533 60-69
ACGGT 11585435 3.5746558 93.87918 40-44
TCTTG 11292305 3.5730011 19.668331 170-179
TGTCT 11280255 3.5691884 21.061039 180-189
AGATA 6806060 3.4794579 18.445452 150-159
AGGCT 11187255 3.4517984 44.86397 30-34
GAGGA 10705450 3.4068604 129.59886 35-39
GCCCT 11895220 3.3644228 33.72995 20-24
TGTCC 11187190 3.3466887 19.28584 120-129
CCAGG 11329005 3.304893 39.559162 45-49
CTTCA 8015635 3.261622 17.07271 200-209
TCAGG 10484595 3.2349944 20.299772 140-149
CTCTC 9634565 3.2183645 13.997061 100-109
TTCCC 9618870 3.2131217 55.784954 50-59
ATGCA 7645220 3.2085824 77.32607 25-29
GATGG 11595105 3.203965 33.152184 15-19
CCGTG 12559465 3.1812692 24.361107 120-129
GGGTT 13243920 3.177556 43.01901 50-59
CCTGG 12523000 3.1720328 32.051205 50-59
ACAGA 6509015 3.1461167 14.8446245 140-149
TCGCA 8964100 3.088427 25.040232 100-109
CGCAC 9269175 3.019364 25.402525 100-109
TGGAG 10863920 3.0019236 32.085194 25-29
GACTC 8682430 2.9913826 21.355223 190-199
GGATG 10816945 2.988943 55.86359 20-24
TCTCA 7313955 2.976103 20.844257 130-139
GGTTC 11105505 2.9752524 48.588524 50-59
TACAC 6340760 2.9714835 22.978624 110-119
TGCAC 8555065 2.9475012 100.406784 25-29
CCAGT 8540850 2.9426036 41.391666 60-69
TCTCT 8293620 2.9302418 15.904139 180-189
TGGCC 11444855 2.8989425 39.044952 60-69
TTCTT 7695620 2.8758032 21.835678 170-179
CAGAT 6840310 2.8707743 15.713579 150-159
AGGGT 10093145 2.7889423 51.066498 50-59
GTTCC 9311505 2.7855709 55.33134 50-59
CCTGA 8079495 2.7836516 65.548134 30-34
GCCGT 10970675 2.778834 23.164562 120-129
TCACA 5889020 2.759784 12.543971 130-139
CGGTG 12093195 2.7432258 67.983406 40-44
AATAA 3920410 2.726143 13.6017885 110-119
TCAAA 4770515 2.7232647 56.309666 70-79
CGGAT 8777605 2.7083073 63.168285 20-24
GCACT 7846550 2.7033947 70.869514 25-29
AGGGA 8439175 2.6856499 10.063602 150-159
ACCAC 6060210 2.6851187 15.551308 230-239
GGTTG 11185585 2.6837087 57.094875 15-19
CGGCC 11023340 2.6398926 11.011811 120-129
GGCTG 11513370 2.611698 24.94483 30-34
AGTCA 6169325 2.589172 29.802078 70-79
AGATG 6848110 2.5738611 17.323593 180-189
CTGGC 9784710 2.4784331 34.9207 60-69
TGTTC 7788515 2.4643657 12.830855 140-149
GAGAT 6552885 2.462901 16.809675 180-189
GTTGG 10250495 2.4593568 44.631943 15-19
GATGC 7817990 2.4122205 73.03337 25-29
ACTCT 5867490 2.3875256 13.264254 200-209
GTCTC 7928920 2.3719654 12.724188 180-189
CAGAG 6648980 2.3627255 9.61619 140-149
ACAGC 5946575 2.3595755 12.644759 120-129
GTTCT 7438270 2.353545 18.528858 160-169
TTGTC 7418875 2.3474078 15.268632 170-179
TCATT 5419685 2.332523 16.427523 150-159
CATTT 5373460 2.3126287 17.675339 150-159
CTGGA 7463930 2.302976 15.404363 180-189
TTCAT 5331875 2.2947316 17.221481 140-149
TCCAT 5632850 2.292049 13.022577 70-79
TGTAG 6984600 2.279395 8.956795 210-219
TCCTC 6796310 2.2702637 14.96306 130-139
ATATA 3720350 2.2462838 10.526211 110-119
TTGGT 7891985 2.2362893 41.527477 25-29
CACTA 4719300 2.2116156 15.060654 240-247
GTGAA 5846005 2.1972203 17.3164 190-199
AATAT 3623290 2.1876807 12.713183 110-119
GGCGG 11385835 2.1868565 35.423916 20-24
TAGTC 5993105 2.1839302 25.146782 70-79
TTCAC 5360075 2.1810548 17.481403 200-209
ATTTG 5533765 2.132867 15.593848 150-159
TGAAT 4804005 2.13247 14.913943 190-199
ACTAC 4544075 2.1294992 12.322089 230-239
TTGGA 6509920 2.1244853 17.211103 170-179
GCGGA 8095815 2.1150343 75.95412 20-24
GAATC 5025810 2.109256 15.233597 190-199
CAGCC 6472235 2.108282 11.619533 120-129
GAGTC 6822845 2.105171 12.649706 210-219
CTCTG 7009315 2.0968623 15.27167 180-189
CTCGC 7347580 2.0781763 17.380941 100-109
GATAC 4947935 2.0765731 13.314641 150-159
ATATG 4660705 2.0688598 9.927445 180-189
GTCAA 4925025 2.066958 40.48245 70-79
TCTGC 6879930 2.0581563 10.765579 210-219
TCGGC 8106080 2.053242 8.726261 190-199
CTACT 5040260 2.0509195 8.241582 230-239
CCTTG 6846185 2.0480614 42.99733 20-24
TCTCG 6828355 2.0427275 16.838722 100-109
AATAG 3962875 2.0259383 8.219112 240-247
GTTCA 5555165 2.024342 15.297698 140-149
CCTTC 6041350 2.018074 13.911352 200-209
AATCG 4788470 2.009648 13.921316 190-199
ACACA 3721650 2.0086484 14.25131 110-119
ATAGA 3926705 2.007447 10.2677145 240-247
GATAT 4517545 2.005312 9.838732 180-189
TACAG 4695240 1.9705207 11.888093 160-169
CACGG 6723300 1.9613185 7.46161 200-209
TCCCA 5073500 1.951853 12.057637 240-247
TTGGG 8091750 1.9414184 70.805374 15-19
GCTGA 6286765 1.9397649 39.378128 30-34
GTTGT 6803175 1.9277619 7.8767624 210-219
GGTCA 6230860 1.9225155 10.1956 130-139
TTGCA 5271395 1.920934 13.7451725 150-159
TTTGC 5990330 1.8954017 11.953287 150-159
TACTA 3821495 1.8941813 10.717215 230-239
TGGAA 5022465 1.887693 9.565425 25-29
AGTAT 4251055 1.8870187 12.102392 220-229
GCTGT 7040465 1.8861961 10.104902 140-149
ATTGT 4883380 1.8821907 8.628537 170-179
GTCCT 6267275 1.8748783 12.815256 130-139
AAAAA 2340985 1.8747879 6.3780165 230-239
GTCAC 5420830 1.8676542 9.510024 130-139
TACCA 3965330 1.8582808 11.90571 230-239
GAACT 4423040 1.8562826 11.139188 160-169
CAGGC 6279895 1.8319685 14.145824 140-149
AGTCT 5021025 1.8296975 13.092416 210-219
CATAG 4310505 1.8090533 8.089206 210-219
ATGTA 4033690 1.7905315 10.985698 70-79
CTGTT 5637540 1.7837756 12.526223 140-149
CACCA 3987695 1.7668421 11.531204 230-239
TTGTA 4580580 1.7654829 10.439843 210-219
ATAGT 3952060 1.7542965 7.4286485 220-229
AGAAC 3621310 1.7503514 11.178945 160-169
TCAGC 5073125 1.7478584 11.565651 140-149
GTCTG 6451705 1.7284628 8.96223 210-219
CCAGA 4350235 1.7261547 23.723906 60-69
GTGGA 6206370 1.7149471 39.672577 25-29
TCTGG 6391285 1.7122757 12.394981 180-189
TGGGG 8421395 1.7107862 42.25957 15-19
GCAGA 4785230 1.7004389 13.707397 150-159
CAGCT 4904615 1.6898013 9.414835 150-159
GACGT 5470135 1.6877959 10.388229 170-179
CCCAC 4593245 1.6707169 10.8976145 240-247
ATAAT 2763995 1.668853 5.8423433 110-119
ACGGC 5692150 1.6605119 12.370168 120-129
TGACT 4549215 1.6577666 12.191523 190-199
TTCAG 4539150 1.6540987 10.33783 150-159
GGACG 6319060 1.6508561 8.17158 170-179
ATCTC 4053790 1.6495175 7.162672 100-109
GAGAA 3810660 1.6494949 10.980367 160-169
GGAGG 7045005 1.6482736 27.280674 30-34
TGCAG 5317500 1.6407006 12.934268 150-159
CTCCA 4241675 1.6318372 8.517798 240-247
AACTG 3851430 1.6163867 10.860902 160-169
AACCA 2994530 1.6162072 12.3126335 70-79
CGACT 4620515 1.5919195 9.989885 190-199
GTCCC 5627340 1.5916268 17.178076 50-59
GGGGC 8248045 1.584187 39.037884 15-19
ATAAA 2277780 1.5839043 6.8909864 110-119
CAAAA 2405210 1.5812987 20.595135 70-79
ACGGA 4425485 1.572603 10.009577 200-209
CACTG 4546580 1.5664463 6.806112 230-239
TCGAC 4533730 1.562019 10.350443 190-199
CAAAG 3216040 1.5544653 28.620739 70-79
TGGTT 5479565 1.5527011 15.994233 10-14
ATGGG 5598195 1.5468957 54.38134 15-19
AGCTC 4484080 1.5449129 9.6995535 140-149
CTCTT 4360350 1.5405673 9.366022 200-209
ACTGA 3662970 1.5372927 7.5470796 180-189
CCTCG 5377295 1.5209045 9.284165 130-139
GGGCG 7897840 1.5169237 45.737343 20-24
AAATA 2133730 1.4837359 5.182325 110-119
TCAGA 3528635 1.4809145 8.870314 130-139
AGCCG 5061760 1.4766148 11.193816 120-129
GCTCT 4902215 1.4665155 9.421731 130-139
TGGAC 4749660 1.4654952 11.365747 170-179
CTGGT 5470130 1.4654909 7.131506 160-169
CTCGA 4246485 1.4630537 9.570842 190-199
TCCAC 3796690 1.4606447 10.483222 240-247
AATTG 3259665 1.4469464 9.382139 170-179
GAGGG 6173345 1.4443371 9.721029 200-209
CCACC 3969535 1.4438527 8.343979 230-239
ATCGG 4641520 1.4321288 9.549118 190-199
GGCCG 6641595 1.4244153 11.791438 120-129
CCATC 3691920 1.4203382 11.003752 240-247
ACACG 3568920 1.4161323 9.152189 120-129
TAGTT 3644420 1.404661 8.937031 220-229
CGTAG 4521490 1.3950938 8.232875 210-219
CAATA 2429700 1.3870025 7.7429323 100-109
CTTGA 3787230 1.3800938 9.802322 200-209
ACTAT 2776045 1.3759882 7.487076 230-239
GGGAG 5861550 1.3713883 7.0713067 150-159
GAATT 3073165 1.3641602 10.408856 170-179
TATGG 4179640 1.3640081 7.184304 180-189
CAGAC 3387365 1.3440921 12.283222 60-69
CATGT 3675425 1.339351 10.520214 70-79
ACTCG 3844195 1.3244516 9.721007 190-199
GGCTC 5227105 1.3240076 8.411477 130-139
GAGGC 5060615 1.3220874 23.937592 30-34
ACCAT 2806970 1.3154362 10.414189 45-49
ACGTG 4263055 1.3153546 9.821758 170-179
TATAC 2641375 1.309237 6.435847 110-119
AAGTA 2539310 1.2981699 17.435127 70-79
GTGCT 4811485 1.2890348 7.5092607 220-229
ACTTC 3160320 1.2859579 5.7393203 220-229
ATCCA 2735620 1.2819992 16.469643 240-247
TTCCA 3141780 1.2784138 7.600825 230-239
GGTGG 6282090 1.2761914 39.36958 25-29
GGGCC 5926745 1.2711022 30.645374 15-19
ACTGT 3483250 1.2693211 5.3161497 230-239
CCATG 3683985 1.269254 10.629831 70-79
TGATA 2836150 1.2589506 7.090085 180-189
GCTCA 3641580 1.254644 9.582247 140-149
AAAGT 2450230 1.2526295 21.086311 70-79
GTCCA 3627595 1.2498257 12.008578 70-79
GTGTT 4398850 1.2464674 7.304974 160-169
TTGAG 3784720 1.2351274 8.914509 200-209
GAACC 3112575 1.2350564 9.200312 70-79
TGTGC 4604895 1.2336878 6.371018 220-229
TATCA 2484500 1.2314795 5.7284093 70-79
TGGGC 5417595 1.2289298 32.76648 15-19
AGAGA 2832680 1.2261631 12.660565 35-39
CCTCA 3184020 1.2249411 5.786998 130-139
GGAGT 4424005 1.222443 6.8506064 210-219
AGTTC 3277025 1.1941714 5.859956 160-169
GGAAT 3163895 1.1891495 8.314809 170-179
GCGTA 3850215 1.1879739 9.096872 210-219
CTGAT 3244350 1.1822642 6.096894 180-189
ACTGG 3783190 1.1672933 7.1984506 160-169
CTGCG 4598135 1.1646918 6.3063254 210-219
GTCTA 3195650 1.1645178 7.8075423 180-189
ACAAT 2032815 1.1604394 8.963456 100-109
TGCCC 4095545 1.1583766 9.892795 60-69
TCACG 3332500 1.1481558 8.61006 200-209
AGATC 2710415 1.13752 5.3088765 130-139
AGTGA 3018555 1.1345236 5.0442514 160-169
GCATA 2678595 1.1241655 7.4831223 210-219
TATGT 2916625 1.1241485 7.518843 220-229
CTGAA 2653360 1.1135747 13.616411 35-39
TAGAT 2497960 1.1088299 8.331792 240-247
CCCAA 2502065 1.1085988 9.944826 240-247
TCTAC 2698450 1.0980196 7.375562 180-189
GCGGT 4807870 1.0906193 7.2524037 130-139
CCAAT 2302335 1.0789481 11.166524 240-247
CGGCT 4254730 1.0777085 7.6893673 130-139
TATAA 1782675 1.0763487 7.352028 240-247
CTCGG 4220950 1.0691521 7.5903473 130-139
TTGGC 3986260 1.0679505 5.051237 170-179
TGGTC 3979320 1.0660913 8.693948 50-59
AGGTT 3265515 1.0656872 17.885595 10-14
AAAAG 1791110 1.0545676 13.349594 70-79
ATAAC 1830670 1.0450442 7.275445 80-89
GAAGA 2397210 1.0376642 17.865314 35-39
GTGGT 4314305 1.0351125 7.655629 50-59
AGAGC 2902860 1.0315356 6.5305243 140-149
CGGTC 4009085 1.0154874 7.4403934 130-139
TACTG 2722930 0.9922552 5.244878 180-189
CGATG 3194160 0.98554975 22.772701 15-19
CGGAG 3755445 0.9811113 6.489377 210-219
CATCA 2091615 0.9801979 5.7356653 230-239
GCTTC 3242615 0.97004014 5.6612344 150-159
ATATC 1955780 0.9694117 5.7066836 70-79
AGCTT 2658265 0.9686908 5.902078 150-159
TGTAA 2180155 0.9677582 6.5452075 230-239
TCTGT 3055420 0.96676624 5.340436 210-219
GAGTT 2957815 0.9652706 5.3071184 160-169
GTCAG 3121120 0.96301335 5.135243 130-139
ACCGT 2773780 0.9556584 13.600112 45-49
AAGAG 2200740 0.9526196 12.374305 35-39
ACCCA 2137375 0.9470143 11.632084 240-247
ACTAA 1636650 0.93428713 11.541272 240-247
TAGAC 2209810 0.9274236 5.028301 110-119
TCCAG 2654530 0.91457283 5.6776524 240-247
TGAAG 2425105 0.91147536 9.591518 35-39
CTTCC 2720805 0.90886736 5.4021378 230-239
CTACC 2348375 0.9034558 6.7314773 230-239
ATCAA 1521255 0.8684135 12.65636 70-79
CCAGC 2650615 0.8634179 5.911943 80-89
GAGCT 2788885 0.8605032 5.582048 140-149
TGCAT 2352270 0.85718393 6.1198826 210-219
AGCTG 2753460 0.8495729 7.5720344 30-34
TGCGT 3163600 0.8475534 6.819124 210-219
AATCC 1790355 0.83901787 10.603637 240-247
CCTTT 2327065 0.82218176 6.4037614 50-59
CCTCT 2444765 0.816658 5.0079136 100-109
CACAA 1512090 0.81610495 5.2032094 100-109
AGCCA 2024105 0.8031563 7.439819 80-89
TGAAC 1889550 0.7930154 5.443655 190-199
GTATG 2390260 0.78005135 5.077058 220-229
GGAAG 2434185 0.7746454 10.0795355 30-34
TAACC 1647510 0.7720761 6.4104724 80-89
AGACC 1921570 0.7624708 5.3783016 240-247
CAATC 1620005 0.7591864 10.56327 240-247
ACGTC 2181805 0.75170356 8.006896 70-79
CTAAT 1511355 0.7491258 9.007256 240-247
TCGAA 1780550 0.7472697 13.224789 70-79
AGGAT 1986580 0.7466559 8.616148 10-14
CGGCG 3476685 0.7456407 5.218188 130-139
TGAGA 1979815 0.74411327 5.931606 240-247
AAATG 1446920 0.739708 5.3751388 9
AAAGG 1706455 0.73866177 7.2473745 10-14
TAGGT 2261055 0.7378858 6.107828 10-14
GCGGC 3408715 0.73106325 5.401844 130-139
GAAGT 1923625 0.7229942 5.185724 70-79
ATACC 1527850 0.71599954 6.0041795 240-247
GGTCC 2798490 0.7088478 9.663191 50-59
GTCGA 2255405 0.6958993 8.839953 70-79
ACGAT 1640155 0.6883482 8.946191 10-14
CGAAC 1734390 0.6881986 9.09963 70-79
TGGAT 2085935 0.6807362 7.1299505 10-14
GACCG 2316515 0.67577296 13.663331 45-49
AATGG 1758745 0.6610241 5.620045 10-14
CTTTG 2059245 0.65156627 5.578867 50-59
AAAGC 1325140 0.6405032 9.0823345 70-79
CGTGG 2817995 0.63923526 5.15628 50-59
AAGGT 1674725 0.62944514 6.529836 10-14
CGTCC 2132745 0.6032218 6.313746 70-79
CATCC 1565260 0.60217947 7.2905607 240-247
GAAGC 1682950 0.5980388 9.174312 30-34
ATAGG 1558095 0.5856098 5.36928 10-14
TTGCC 1927650 0.5766635 6.5568104 60-69
AAGCT 1345515 0.5646922 15.261593 30-34
TCAAG 1287905 0.5405141 5.8047276 70-79
GACGA 1463670 0.5201174 6.7217617 40-44
GGGAT 1731420 0.47842672 6.065865 10-14
CGGTT 1760495 0.47165054 9.809923 10-14
AAGCA 964095 0.465993 5.6496177 70-79
TCGAT 1203270 0.43848017 7.1229663 10-14
GCGAT 1067205 0.32928333 5.6948705 10-14