FastQCFastQC Report
Sat 8 Jul 2017
HNCHNBCXY_s2_1_illumina12index_8_SL254635.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HNCHNBCXY_s2_1_illumina12index_8_SL254635.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9861787
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11302180 8.252556 58.16221 110-119
CACAG 14840410 7.1540847 41.013687 110-119
TCCCT 16095760 6.507852 95.12322 30-34
CAGTA 12752120 6.492885 43.278587 60-69
ATACA 9147360 6.3237524 32.266888 110-119
GGAGA 15936350 6.17992 92.60772 35-39
AGTAA 9392145 5.8235364 43.456894 110-119
GGTGA 17226965 5.794063 92.4015 45-49
CCCTG 16356000 5.6156373 57.066677 30-34
GTAAT 10133610 5.449626 37.326984 110-119
ACAGT 9751940 4.96531 33.831257 110-119
AGACG 11454805 4.9526634 99.71682 40-44
GTAGT 12386580 4.9060297 25.060066 70-79
GGCCC 16690920 4.86629 57.697483 60-69
GTGAC 12904065 4.8390274 82.3504 45-49
GAGAC 11045295 4.775605 129.78253 40-44
CCCCA 10746080 4.7429514 100.16865 60-69
ACCAG 9135745 4.404049 97.22004 45-49
GTGTC 13491130 4.3879366 25.392185 120-129
AATAC 6343390 4.385312 32.022114 110-119
ACTCC 9289200 4.3303604 95.769394 30-34
CGTGT 13125410 4.2689877 24.47991 120-129
GCACA 8826495 4.254969 33.677498 110-119
TGACC 10003260 4.1824446 150.87175 45-49
GACCA 8436645 4.0670357 108.532715 45-49
TAGTA 7536785 4.053112 25.990543 80-89
CCCTT 9987885 4.0383105 59.62313 20-24
CTCAG 9479280 3.9633641 30.3475 140-149
CCACT 8491145 3.9583294 28.164806 240-247
CACTC 8487590 3.9566722 81.39963 25-29
TGGTG 13450355 3.9236362 26.693678 25-29
AGTAG 8562135 3.910032 28.147436 70-79
CCCAG 9873205 3.908406 82.9809 60-69
TGAGG 11436480 3.8465102 69.657455 35-39
CTCCC 9816685 3.7578886 58.904663 30-34
CAGGG 11576945 3.6865602 34.453423 50-59
GACGG 11484800 3.6572175 111.59066 40-44
AGGAG 9425555 3.6551142 92.34064 35-39
CTGAG 9662265 3.6233516 97.923935 35-39
CTTGG 11047490 3.5931525 35.0225 20-24
ATGGT 9035805 3.5788672 25.571571 190-199
ACGGT 9477400 3.5540276 93.29797 40-44
TGTCT 9267305 3.5495212 20.885052 180-189
TCTTG 9252570 3.543877 19.629602 170-179
GCCCC 10837510 3.5229323 73.53072 60-69
AGATA 5630515 3.491163 18.913742 150-159
GAGGA 8771580 3.4015107 128.79037 35-39
AGGCT 8984690 3.3692613 41.713654 30-34
ATGCA 6590710 3.355734 80.79504 25-29
CTTCA 6734505 3.3158782 17.427418 200-209
TGTCC 9095435 3.2983186 19.585955 120-129
CCAGG 9285765 3.2968717 39.743923 50-59
GCCCT 9497650 3.2609043 31.523838 20-24
TTCCC 8026385 3.2452357 56.266136 50-59
GGGTT 11104020 3.2391808 43.750217 50-59
TCAGG 8597460 3.2240496 20.391222 140-149
CCTGG 10442915 3.2157822 32.021053 50-59
CTCTC 7866970 3.1807804 13.8242445 100-109
CCGTG 10142620 3.1233096 24.44906 120-129
GATGG 9272265 3.118605 31.158434 15-19
ACAGA 5305540 3.1146154 14.984988 140-149
GGATG 9178320 3.0870075 58.456554 20-24
TGCAC 7346015 3.0714285 104.48503 25-29
TCGCA 7304065 3.053889 25.18132 100-109
GGTTC 9307735 3.0273042 49.241528 50-59
CCAGT 7183505 3.0034816 42.114162 60-69
CGCAC 7581850 3.00135 25.668144 100-109
TACAC 5250045 2.9804058 23.35023 110-119
GACTC 7091420 2.9649808 21.14806 190-199
TCTCA 6018460 2.963318 21.393248 130-139
TGGAG 8677120 2.9184356 29.730106 25-29
TCTCT 6787115 2.8984015 15.661586 180-189
TGGCC 9404445 2.8959968 39.27725 60-69
TCACA 5085680 2.8870974 12.6007 130-139
CCTGA 6854745 2.8660247 67.552795 30-34
TTCTT 6288285 2.8363037 21.76847 170-179
GCACT 6765135 2.8285577 74.07856 25-29
AGGGT 8388455 2.821347 51.660034 50-59
GTTCC 7751935 2.811119 56.05588 50-59
CAGAT 5490510 2.7955549 15.682274 150-159
CGGAT 7411180 2.7791944 66.017975 20-24
GCCGT 8982130 2.7659492 23.200987 120-129
GGTTG 9479470 2.7652795 60.145367 15-19
ACCAC 5124490 2.7543297 15.923189 230-239
CGGTG 9845970 2.7193575 67.50722 40-44
TCAAA 3898945 2.6954181 55.993958 70-79
AGGGA 6917610 2.682564 9.929042 150-159
AATAA 3146650 2.6490748 12.624799 110-119
CGGCC 9076760 2.6463578 11.368958 120-129
AGTCA 5161375 2.6279721 30.211103 70-79
GTTGG 8775000 2.5597768 46.626396 15-19
GGCTG 9170100 2.5326893 23.196468 30-34
GATGC 6744610 2.5292304 76.96272 25-29
AGATG 5519740 2.5206752 17.088827 180-189
CTGGC 8073980 2.4862945 35.23269 60-69
TGTTC 6465275 2.4763 13.107218 140-149
GAGAT 5321405 2.4301023 16.775043 180-189
ACTCT 4821995 2.374213 13.036036 200-209
GTTCT 6138910 2.3512974 18.820822 160-169
GTCTC 6475395 2.3482018 12.684671 180-189
AAAAA 2417285 2.3463519 10.952772 230-239
ACAGC 4861825 2.3437295 12.361027 110-119
CAGAG 5394910 2.3325732 9.793654 140-149
TTGTC 6070480 2.3250875 15.334103 170-179
TCATT 4460095 2.3194454 16.346327 150-159
TTCAT 4439460 2.3087146 17.476475 140-149
CATTT 4412360 2.2946212 17.443594 150-159
CTGGA 6108090 2.2905352 15.379782 180-189
TCCTC 5621840 2.2730274 14.862075 130-139
TGTAG 5699700 2.2575154 8.974509 210-219
TCCAT 4565735 2.2480376 12.0304575 70-79
GGCGG 9553965 2.2407181 37.281143 20-24
TGAAT 4162085 2.238275 15.175345 190-199
CACTA 3929360 2.2306643 15.031723 240-247
ATATA 3043005 2.2219226 10.603048 110-119
TAGTC 5027615 2.2202291 25.828154 70-79
TTCAC 4498045 2.2147093 17.83168 200-209
GCGGA 6925410 2.205326 79.30995 20-24
GAATC 4315280 2.1971734 15.518422 190-199
GTGAA 4762270 2.1747649 17.249422 190-199
ACTAC 3794820 2.154287 12.517709 230-239
AATAT 2942720 2.1486971 12.977607 110-119
TTGGT 6237510 2.1427448 38.568893 25-29
ATTTG 4554815 2.124485 15.292264 150-159
TTGGA 5336685 2.113734 17.31123 170-179
CTCTG 5809675 2.1067884 15.182537 180-189
GATAC 4112110 2.093727 13.800496 150-159
GAGTC 5578965 2.0921133 12.687053 210-219
CAGCC 5273930 2.0877373 11.245816 120-129
CTACT 4238605 2.0869682 8.336138 230-239
GTCAA 4070250 2.0724134 41.12521 70-79
TCTGC 5692000 2.0641155 10.919321 210-219
GTTCA 4673760 2.0639644 15.867031 140-149
TCGGC 6646190 2.046622 8.755619 190-199
CTCGC 5958620 2.045821 17.188818 100-109
CCTTC 5024450 2.03149 13.949301 200-209
ATATG 3775915 2.0306015 9.886691 180-189
GATAT 3775440 2.0303462 9.570977 180-189
TTGGG 6959065 2.030046 73.634445 15-19
TCTCG 5578585 2.0229871 16.735926 100-109
AATAG 3251720 2.0162072 8.933532 240-247
ACACA 3029585 1.9829651 13.82849 110-119
ATAGA 3194305 1.9806074 10.299683 240-247
CACGG 5555150 1.9723328 7.683776 200-209
TACAG 3869670 1.9702857 11.97641 160-169
AATCG 3865130 1.9679744 14.059531 190-199
TCCCA 4149210 1.9342434 11.4578905 240-247
GTTGT 5612245 1.9279503 7.607613 210-219
TTGCA 4344870 1.9187243 13.59002 150-159
CCTTG 5268540 1.9105542 39.49624 20-24
TACCA 3357020 1.9057517 12.178944 230-239
GGTCA 5068625 1.9007357 10.144811 130-139
TACTA 3169070 1.9001652 10.912014 230-239
GAACT 3688450 1.8780158 11.71125 160-169
AGTAT 3491860 1.8778433 12.133325 220-229
GTCCT 5158650 1.8707045 12.664062 130-139
GTCAC 4474070 1.870645 9.524234 130-139
ATTGT 3994475 1.8631278 8.858846 170-179
GCTGT 5723340 1.8614937 10.196458 140-149
GCTGA 4935760 1.8509111 36.278038 30-34
TGGAA 4051295 1.8500869 9.282025 170-179
TTTGC 4820380 1.8462801 11.713743 150-159
CAGGC 5146650 1.8272966 14.16266 140-149
AGTCT 4116470 1.8178613 13.281282 210-219
CATAG 3547515 1.8062571 8.562343 210-219
CTGTT 4699540 1.7999965 12.716544 140-149
TCAGC 4294295 1.7954797 11.83855 140-149
TGGGG 7244225 1.7944968 44.306763 15-19
GTCTG 5411475 1.7600609 9.056679 210-219
TTGTA 3770780 1.7587906 10.0996275 210-219
ATGTA 3256115 1.7510649 10.180871 70-79
ATAGT 3255385 1.7506721 7.3008804 210-219
AGAAC 2958290 1.7366631 11.411646 160-169
CAGCT 4096190 1.7126503 9.5972595 150-159
CACCA 3180575 1.7095071 11.3839855 230-239
TCTGG 5239235 1.7040405 12.43043 180-189
GCAGA 3930820 1.6995511 13.960765 150-159
TTCAG 3830275 1.6914757 10.278518 150-159
ACGGC 4751205 1.6868955 12.690476 120-129
ATCTC 3385630 1.6669875 6.825055 100-109
GGGGC 7097715 1.6646469 40.886925 15-19
CCCAC 3761325 1.6601199 11.040672 240-247
TGACT 3750360 1.6561847 12.337337 190-199
TGCAG 4398905 1.6495904 12.856501 150-159
GACGT 4397725 1.6491481 10.289061 170-179
GAGAA 3114135 1.6396652 11.211636 160-169
CTCCA 3511935 1.637164 8.779441 240-247
GGAGG 5699920 1.6279424 25.449045 30-34
AACTG 3196205 1.6273835 10.900985 160-169
TGGTT 4714845 1.6196703 17.403296 10-14
GTGGA 4808935 1.6174221 36.615513 25-29
GGACG 5047105 1.6071991 8.148303 170-179
CACTG 3836295 1.6039861 6.90341 230-239
CCAGA 3303745 1.5926291 22.027424 60-69
AACCA 2431450 1.5914657 12.273476 70-79
GGGCG 6782575 1.5907363 48.39555 20-24
CAAAG 2698840 1.5843531 28.762255 70-79
ATAAA 1873825 1.5775198 6.267537 110-119
ATAAT 2158480 1.5760657 5.4837666 110-119
CGACT 3736640 1.5623195 9.600821 190-199
CAAAA 1951590 1.5555615 20.026024 70-79
TCGAC 3697335 1.5458858 10.362508 190-199
AGCTC 3692075 1.5436867 9.685594 140-149
CTCTT 3610495 1.5418428 9.35726 200-209
ACTGA 3013025 1.5341156 7.609884 180-189
ACGGA 3545745 1.5330582 10.096619 200-209
GTCCC 4449020 1.5275179 16.020006 50-59
CCTCG 4382890 1.504813 9.130976 130-139
CTGGT 4613445 1.5005049 7.2882004 160-169
CTTGA 3391805 1.4978443 9.810801 200-209
TCCAC 3172995 1.4791597 10.629888 240-247
GCTCT 4062415 1.4731718 9.589875 130-139
ACACG 3055675 1.4730427 9.644436 110-119
TCAGA 2892360 1.4726775 8.952411 130-139
GAGGG 5155310 1.4723976 9.697351 200-209
ATCGG 3919395 1.469774 9.718789 190-199
ATGGG 4368320 1.4692272 50.43127 15-19
AAATA 1737400 1.4626675 5.2618527 6
CCACC 3305395 1.4588879 8.445216 230-239
AATTG 2697900 1.4508696 9.842172 170-179
TGGAC 3857325 1.4464978 11.164511 170-179
CTCGA 3441255 1.4388167 9.260469 190-199
GGCCG 5487520 1.43495 12.09479 120-129
AGCCG 4029940 1.4308134 10.773613 120-129
CCATC 3064510 1.4285871 11.042305 240-247
TAGTT 3054040 1.4244844 8.848017 220-229
GGGAG 4905665 1.4010968 7.154056 150-159
CGTAG 3701620 1.3881084 8.341927 210-219
GAATT 2538035 1.3648977 10.777737 170-179
TATAC 2269210 1.3606118 6.73811 110-119
TATGG 3420195 1.3546576 6.917482 180-189
CAATA 1954995 1.351527 7.561248 100-109
ACTAT 2240705 1.3435204 7.133959 230-239
GGCTC 4344880 1.3379587 8.681669 130-139
GTGCT 4066060 1.3224701 7.759821 220-229
ACGTG 3522160 1.320811 9.743369 170-179
ACTCG 3155535 1.319355 9.367846 190-199
CATGT 2980100 1.3160325 9.697738 70-79
ATCCA 2311705 1.3123353 16.79764 240-247
CAGAC 2709720 1.3062689 11.211807 60-69
ACCAT 2290050 1.3000419 9.783217 45-49
AAGTA 2087470 1.2943218 17.4747 70-79
ACTGT 2922685 1.2906777 5.6002436 230-239
ACTTC 2592240 1.276345 5.7577605 220-229
TGATA 2372695 1.2759817 7.0493207 180-189
GCTCA 3049395 1.2749768 9.746894 140-149
GAGGC 3991145 1.2709395 22.378511 30-34
AAAGT 2046870 1.269148 21.458433 70-79
GTGTT 3689045 1.2672817 7.4165206 160-169
TTCCA 2570190 1.2654884 7.3547153 230-239
CCATG 2974330 1.2435915 9.767763 70-79
TGTGC 3812075 1.2398623 6.302938 220-229
GGAGT 3650675 1.2278566 6.936381 210-219
GGAAT 2671185 1.2198381 8.605873 170-179
TTGAG 3078260 1.2192255 8.901829 200-209
CCTCA 2610380 1.2168847 5.926163 130-139
GAACC 2518700 1.2141843 8.992046 70-79
GGTGG 4892135 1.2118509 36.48374 25-29
GGGCC 4581120 1.1979324 28.307226 15-19
CTGAT 2710580 1.1970106 6.2567477 180-189
GTCCA 2860570 1.1960276 11.1767435 70-79
AGTTC 2705190 1.1946304 5.846006 160-169
TATCA 1985760 1.1906561 5.6585374 70-79
ACTGG 3162495 1.1859363 7.291336 160-169
GCGTA 3158555 1.1844589 9.346233 210-219
AGAGA 2234050 1.1762798 12.278709 35-39
TGGGC 4175910 1.1533443 30.267689 15-19
CTGCG 3742680 1.1525177 6.6110845 210-219
TCACG 2749230 1.1494755 8.894646 200-209
GTCTA 2602825 1.1494254 7.884004 180-189
CTGAA 2227555 1.1341846 13.27853 35-39
TGCCC 3280900 1.1264578 9.332027 60-69
TATGT 2410015 1.1240941 7.7026253 220-229
TAGAT 2083085 1.1202359 8.248591 240-247
GCATA 2197880 1.1190752 7.667335 210-219
TCTAC 2270670 1.1180131 7.600688 180-189
ACAAT 1616365 1.1174253 8.4529705 100-109
CCCAA 2068060 1.1115484 9.840282 240-247
AGATC 2170285 1.105025 5.624075 130-139
GAAGA 2084875 1.0977356 17.641632 35-39
AGAGC 2499035 1.0804967 6.774923 140-149
AGGTT 2726260 1.0798067 18.586185 10-14
GCGGT 3904195 1.0782993 7.154024 130-139
CGGCT 3480050 1.0716436 7.7046227 130-139
CCAAT 1878515 1.066417 11.13156 240-247
CTCGG 3456840 1.0644963 7.582002 130-139
TATAA 1451940 1.0601685 7.570943 240-247
TTGGC 3247790 1.0563309 5.0151353 170-179
AAAAG 1472605 1.0527562 13.256669 70-79
ATAAC 1498165 1.0357113 7.1012497 80-89
TGGTC 3155585 1.0263416 7.998669 50-59
GTGGT 3498125 1.0204467 7.0127687 50-59
CGGTC 3271235 1.0073413 7.2634325 130-139
AAGAG 1912930 1.0072025 12.203295 35-39
TACTG 2280695 1.0071704 5.284911 180-189
TGTAA 1871180 1.0062782 6.971389 230-239
ATATC 1661420 0.99618274 5.7145085 70-79
TCTGT 2592160 0.99283737 5.406384 210-219
CGGAG 3096380 0.98601055 6.52787 210-219
GCCAC 2489095 0.9853328 5.067977 80-89
CATCA 1734720 0.98478585 5.696828 230-239
GCTTC 2713920 0.984161 5.7715387 150-159
AGCTT 2223305 0.9818268 6.1416607 150-159
ATACG 1917850 0.9764949 5.022088 120-129
GAGTT 2431965 0.9632434 5.4534526 160-169
ACCCA 1782665 0.9581533 12.3207855 240-247
ACTAA 1377470 0.9522725 11.866369 240-247
CTACC 1983495 0.92464876 6.8288703 230-239
TCCAG 2203330 0.9212302 5.6475873 240-247
CGATG 2446190 0.9173218 21.325163 15-19
TAGAC 1786780 0.9097591 5.047116 110-119
CTTCC 2248725 0.9092065 5.1792817 230-239
ACCGT 2174300 0.9090925 12.819611 45-49
TGAAG 1952045 0.8914317 9.420274 35-39
GAGCT 2353340 0.88250315 5.588634 140-149
CCAGC 2213665 0.8763011 5.84121 80-89
ATCAA 1239430 0.8568426 12.33826 70-79
TGCAT 1928760 0.85175353 6.2689295 210-219
AGCTG 2267290 0.8502344 6.8432026 30-34
AATCC 1497445 0.8500868 10.696546 240-247
TGCGT 2599455 0.8454625 7.180381 210-219
TGAAC 1649715 0.83997095 5.439017 190-199
AGCCA 1702385 0.8206651 7.74888 80-89
GTATG 2049985 0.81195027 5.2446795 220-229
AGACC 1658050 0.7992926 5.454733 240-247
CCTTT 1845370 0.7880555 6.1446767 50-59
CTAAT 1307020 0.7836854 9.524092 240-247
AAAGG 1474570 0.77639574 7.461342 10-14
GGAAG 1979980 0.7678119 9.562737 30-34
TAACC 1339765 0.7605732 6.5360966 80-89
CAATC 1325730 0.7526056 10.639496 240-247
ACGTC 1786510 0.74695444 7.3592515 70-79
TAGGT 1858405 0.73607 6.234258 10-14
TGAGA 1600555 0.73091835 5.9284353 240-247
TCGAA 1432185 0.72921306 12.805962 70-79
ATACC 1276095 0.7244283 6.4589925 240-247
AGGAT 1572050 0.71790105 8.156193 10-14
GCGGC 2702025 0.7065615 5.1022944 130-139
TGGAT 1764110 0.698722 6.597619 10-14
AGCGT 1850680 0.6940055 5.0606155 160-169
GTCGA 1807305 0.6777398 8.790365 70-79
GGTCC 2193505 0.6754661 8.859491 50-59
GACCG 1895260 0.67290413 13.015117 45-49
AATGG 1449610 0.6619869 5.348071 10-14
CGAAC 1361380 0.6562775 9.003773 70-79
ACGAT 1263280 0.6432132 8.180821 10-14
AAGGT 1402530 0.6404871 6.818272 10-14
CATCC 1321260 0.6159338 7.334935 240-247
CTTTG 1595130 0.6109594 5.3172483 50-59
AAAGC 1038155 0.60944855 8.387384 70-79
AAGAA 827000 0.5912171 5.0269666 7
ATAGG 1244445 0.5682951 5.3362303 10-14
CGTCC 1652770 0.56745887 5.816647 70-79
GAAGC 1307365 0.5652596 8.774416 30-34
TTGCC 1526105 0.55341834 6.29917 60-69
TCAAG 1085135 0.5525086 5.8823314 70-79
AAGCT 1055730 0.53753674 14.176374 30-34
GAAAA 731495 0.52294123 5.696052 6
GACGA 1188330 0.513793 6.482699 40-44
GGGAT 1430980 0.48129132 5.932084 10-14
CGGTT 1451015 0.4719369 9.588522 10-14
AAGCA 785875 0.4613476 5.5185227 70-79
TAAAG 743970 0.4612936 5.620917 9
TCGAT 955655 0.42202377 6.498125 10-14